Page 1
Interceptor
Biological Safety Cabinet
Class II B2
120 VAC
User & Operation Manual
INT-1400B & INT-1400B-1
®
INT-2000B & INT-2000B-1
KEWAUNEE
®
BSCUO-B2120-0714
...encouraging new discovery
Page 2
Page 3
Table of Contents
CHAPTER 1
Product Description 1.1
Technical Specications 1.3
CHAPTER 2
Safety Ergonomics and Reliability 2.1
Safety Ergonomics and Reliability Tips 2.2
Room Location 2.3
Placement Requirements 2.4
Recommended BSC Placement 2.5
CHAPTER 3
Unpack and Inspection 3.1
Assembly and Setup 3.1
Service Feed Requirements 3.6
Exhaust Connection 3.8
CHAPTER 4
Cabinet Operation 4.1
Startup Procedure 4.2
Air Flow Patterns 4.3
Operating the BSC 4.4
Recommended Workow 4.5
Control Monitor 4.6
UV Light 4.6
Control Monitor Messages and Meanings 4.7
Control Monitor Screens & Menu Diagrams 4.8
Chapter 5
Care & Maintenance 5.1
Required Maintenance 5.2
Maintenance Log 5.3
Disinfection, Storage, Filters, and Lamps 5.4
Decontamination 5.5
CHAPTER 6
Operator and Product Protection 6.1
CHAPTER 7
Troubleshooting and Technical Support 7.1
Detailed View 7.2
Replaceable Parts List 7.3
Labels 7.4
Wiring Schematics 7.6
Warranty 7.8
Warranty Registration 7.9
Page 4
NOTICE:
The Interceptor® Biological Safety Cabinet offers many features to enhance
performance, safety, and operator comfort.
Due to the nature of work performed in a Biological Safety Cabinet, it is very
important to read the User and Operation Manual and Maintenance and Technical
Manual and follow standard operating procedures to avoid infection and other
potential injuries.
If this equipment is used in a manner not specied by the manufacturer in this
manual, the protection provided by the equipment may be impaired.
®
Maintenance or service to an Interceptor
instructions contained herein. Maintenance of this product must be carried out by
technicians trained in the mechanical details of this unit.
! WARNING
ê
Class II B2 Biological Safety cabinets must be certified before initial use, after
being moved, and after any service, including required annual recertification.
Service must be performed by an NSF accredited certifier using NSF/ ANSI 49
criteria and should include, at minimum, the following test:
• Downflow Velocity Profile Test
• Inflow Velocity Test
• Airflow Smoke Pattern Test
• HEPA Filter Leak Test
product must be done according to the
Page 5
User and Operation Manual Chapter 1
CHAPTER 1
Product Description
Interceptor® Class II, B2, 120 VAC Biological Safety Cabinet manufactured by
Kewaunee Scientic Corporation
Part Numbers:
• INT-1400B INT-B1400 Biological Safety Cabinet
with 050200-1400 Manually Adjustable Stand
• INT-1400B-1 INT-B1400 Biological Safety Cabinet
with 050200-1400-E Electrically Adjustable Stand
• INT-2000B INT-B2000 Biological Safety Cabinet
with 050200-2000 Manually Adjustable Stand
• INT-2000B-1 INT-B2000 Biological Safety Cabinet
with 050200-2000-E Electrically Adjustable Stand
Product comes standard with:
• Supply and exhaust HEPA lters
• Two (2) electrical ground fault protected duplex receptacles
• Base Stand (choice of electrically or manually adjustable height)
• Armrest
Optional Features:
• Base stands are available in both electrically and manually adjustable height
®
versions. Interceptor
other but both stands are available individually.
• Casters
• Footrest
• Additional Service Connections (up to four total)
• UV Light
• IV Pole
Biological Safety Cabinets are shipped with one or the
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
1.1
Page 6
Chapter 1 User and Operation Manual
1.2
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 7
User and Operation Manual Chapter 1
Technical Specifications for Interceptor
Product
Number
INT -1400B
120 VAC
INT- 2000B
120 VAC
Table 1.1: Product Descriptions
Width of
Superstructure
1400 mm
55.1”
2000 mm
78.7”
Height of
Superstructure
1610 mm
63.4”
1610 mm
63.4”
Depth of
Superstructure
813 mm
32”
813 mm
32”
Fan
Max HP
0.5
0.0
Fan and Light
Amps / Power
5.4 Amps
650 VA
10.9 Amps
1320 VA
®
Outlet Rating
Amps / HZ
20 Amps
60hz
20 Amps
60hz
Exhaust
CFM
755
1120
NOTES:
The powered base stand, if ordered, will take an additional 8.6 amps at 120 VAC; 60 Hz.
®
The electrical outlets inside the Interceptor
are grounded. This is particularly important since all internal surfaces
are stainless steel and conduct electricity. Under NO CIRCUMSTANCES use ungrounded plugs in these outlets.
It is not recommended to use in excess of 1500 watts of power. Exterior power plugs must not be removed until
unit fan and lights are turned off. The unit is to be disconnected from the main voltage by unplugging both plugs to
remove power. For electrically-powered base stands, power for disconnect is also accomplished by plug removal
from a waste-high or lower plug outlet.
The Interceptor® has one power cord. If power base is employed, a total of two power cords are used. When positioning the
BSC, always connect BSC and stand power plugs in waste-high or oor-positioned outlets to facilitate disconnection in an
emergency. Never block these outlets.
If UV option is on your BSC, be sure safety overrides are never immobilized! UV lamp should NEVER be on while
sash is open. Blower must be disabled before UV is activated.
Based on the following UL denitions, the Interceptor® may be used in a room with pollution degree 1 or pollution
degree 2 conditions:
Pollution Degree 1 No pollution or only dry, non-conductive pollution occurs. The pollution has no inuence.
Pollution Degree 2 Normally only non-conductive pollution occurs. Occasionally, a temporary conductivity
caused by condensation must be expected.
Pollution Degree 3 Conductive pollution occurs, or dry, non-conductive pollution occurs which becomes
conductive, which is expected.
Be aware that the dimensions of a Biological Safety Cabinet may exceed doorway limitations.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
1.3
Page 8
Chapter 1 User and Operation Manual
i Attention
¡
Because of the weight and size of the Biological Safety Cabinet and the stand, it
is recommended that they be moved and lifted only with help of a furniture dolly
and/or floor jack. Cabinet and stand weights are listed in the table below:
Interceptor® Biological Safety Cabinet Weights
Cabinet Stand BioSafety Cabinet
Part Number
Shipping
Weight
Installed
Weight
Shipping
Weight
Installed
Weight
INT-1400B
INT-2000B
Table 1.2
i Attention
¡
Do not lay the Biological Safety Cabinet on its side!
207 lbs 147 lbs 765 lbs 704 lbs
255 lbs 168 lbs 1050 lbs 987 lbs
1.4
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 9
User and Operation Manual Chapter 2
CHAPTER 2
Safety, Ergonomic, and Reliability Features
Kewaunee’s Biological Safety Cabinet product line is engineered to provide the utmost
in reliability; a product that is carefully designed with many enhancements to ensure
the operator’s safety.
• 10° Angled Sash for ease of viewing and superior ergonomic working conditions.
• Easy to read factory calibrated Control Monitor indicating average inow
velocity and lter loading.
• Touch Pad Control Panel on right facia of cabinet ergonomically designed for
ease of access; ADA compliant.
• Audible and visual alarms alerting user of unsafe sash height. Sash should
only be operated at 8" opening.
• Dual HEPA lters with minimum 99.99% efciency at 0.3 microns.
• Angular front intake grill design for improved down ow capture and
containment. Ergonomically designed for operator comfort.
• Classic double wall construction fully surrounds containment area with
negative pressure.
• Forward positioning of service ttings for improved ergonomics.
• Reinforced nylon sash belt and sprocket system. Sash anti-racking design will
eliminate binding or slipping.
• Optional bases with electrically or manually controled height adjustment.
Range of adjustment for better ergonomics; ADA compliant.
• UV timer allows user to preset UV illumination cycle, effectively extending lamp
life.
• NSF, UL, CUL Certied.
• Factory Inspected to exceed NSF requirements.
• T5 uorescent light. Energy efcient with brighter illumination than T8 bulb.
• Right and left sidewall position of electrical outlets. Facilitates convenience of
electric connection.
• Armrest. Promotes good working posture and reduces stress points.
®
• Reduced noise and vibration. Interceptor
acceptable noise and vibration levels. Allows operator to work longer and more
comfortably.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
design exceeds NSF standards for
2.1
Page 10
Chapter 2 User and Operation Manual
Tips
The following are recommended for control of ergonomic-related issues associated
with the use of Biological Safety Cabinets:
• Use an ergonomically designed chair that provides adequate back support,
adjustable seat angle, and height adjustability between 18 inches to 28 inches.
• Place anti-fatigue matting in areas for users who must stand for extended
periods of time.
• Place working materials in recommended order to create ‘ow’ “clean to dirty”.
• Take frequent mini-breaks to perform stretching.
• Proper posture should always be practiced. Use of footrest when working
seated is recommended.
• Upper shoulders and upper arms should be relaxed; ensure chair seat height
is aligned properly to provide no stress to upper shoulders or upper arms when
forearms are resting on tabletop (wrist should always align with forearm – think
straight, horizontal line).
2.2
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 11
User and Operation Manual Chapter 2
Room Location
It is imperative that the Biological Safety Cabinet be placed in the correct location
within the room. Ideally the location of the cabinet will be away from any type of room
air turbulence and out of high trafc areas within the laboratory. Air turbulence can
be created by a number of things including; but not limited to, air ducts, doorways,
windows and foot trafc. Placing the cabinet in direct path of unstable air ows can
cause contamination of the cabinet workspace and/or allow contaminates to escape
from the cabinet into the room.
It is also important to keep the proper distance away from the ceiling when
exhausting back into the room. The minimal acceptable height is 3 inches (8cm) from
the ceiling. Less than 3 inches (8cm) clearance constricts the intake and alters the
ow into the cabinet.
First and foremost always refer to your facilites Standard Operating Procedures
(SOP). Always use best housekeeping practices. Never store items on top of the
cabinet. Never remove the exhaust lter cover unless cabinet is being certied by a
technician. If you have questions about cabinet location, please contact Kewaunee
Scientic Corporation at www.kscmarketing@kewaunee.com or 704-873-7202.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
2.3
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Chapter 2 User and Operation Manual
Placement Requirements
Recommended
Location
Air vents blocked or
redirected to prevent
cabinet disruption
Figure 2.1: BSC Placement Example
Refer to NIH recommendations shown on the following pages from Biological Safety
Cabinet (BSC) Placement Requirements for New Buildings and Renovations, National
Institute of Health, Division of Technical Resources, Ofce of Research Facilities.
2.4
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 13
User and Operation Manual Chapter 2
Recommended BSC Placement
Workplace Specication
DO Maintain an undisturbed
space of 40" around BSC
Figure 2.2
Distance to Adjacent Wall
Distance to Opposing Wall
DO Maintain a distance of
12" to adjacent walls
Figure 2.3
Do Place BSC at least 80"
from opposing wall
Figure 2.4
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
2.5
Page 14
Chapter 2 User and Operation Manual
Recommended BSC Placement
Distance to Opposing Bench Top
DO Place BSC at least 60"
to opposing bench top or
area with occasional trafc
Figure 2.5
Distance to Adjacent Bench Top
Distance to Columns
DO Maintain a distance of
40" to adjacent bench top
Figure 2.6
Do Maintain a distance of
12" to columns to avoid
disturbance of BSC airow
2.6
Figure 2.7
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 15
User and Operation Manual Chapter 2
Recommended BSC Placement
Distance to Columns
NOTE: Columns can aid in
dening trafc routes
Figure 2.8
BSC Placement Along Opposing Walls
BSC Placement Along Same Wall
DO Maintain a distance
of 120" between opposing
BSCs
Figure 2.9
Do Maintain a distance of
40" between BSCs along the
same wall
Figure 2.10
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
2.7
Page 16
Chapter 2 User and Operation Manual
Recommended BSC Placement
BSC Placement Along Perpendicular Walls
DO Maintain a distance of
48" between BSCs
Figure 2.11
2.8
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 17
User and Operation Manual Chapter 3
CHAPTER 3
Unpack and Inspection
When unpacking your new Interceptor® Biological Safety Cabinet, it is imperative
that a thorough inspection be performed to ensure that there is no freight damage.
If any damage is found at time of delivery, it should be noted with the carrier on
paperwork. If damage is concealed owner must contact the freight carrier within 15
days of delivery per the United States Interstate Commerce Commission rules and
regulations. Kewaunee Scientic Corporation and its dealers are not responsible for
damages occurring during shipment.
Assembly and Setup
The Biological Safety Cabinet should be maneuvered, as close as possible, to its nal
location with the assistance of either a furniture dolly or oor jack. Attempts to move
the unit by tilting it onto a hand truck greatly increases the risk or injury to the
handler and damage to the Biological Safety Cabinet.
i Attention
¡
A Class II, B2 Biological Safety Cabinet requires that it be connected to an external
exhaust connection. Make sure that an exhaust system with enough capacity is
available before beginning installation. Ductwork must be available for connection.
Miscellanious Parts Packed in Box Inside Cabinet
Qt Part Number Description Qt Part Number Description
1 BSCUOB2-120 User & Operation Manual 3 050195-0A Arm Rest Bracket
1 BSCMTB2-120 Maintenance & Technical Manual 2 F-7279-00 Arm Rest End Caps
1 050110-00 Drain Plate with 3/8" Female Fitting 10 F-3808-00 1/4”-20 Hex Nuts
1 F-7211-00 Type 316 SS Pipe Fitting 4 F-5294-00 5/16" Machine Bolt
1 F-7210-00 Type 316 SS Ball Valve 10 970406 5/16" Flat Washers
3 970442 #8x1/2” Self-drilling Screws
Table 3.1
Parts Packed Loose Inside Cabinet
Qt Part Number Description
1 F-7280-(Length) Aluminum Armrest Tube
Table 3.2
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
3.1
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Chapter 3 User and Operation Manual
Step 1 Remove End Panel
Remove the ve (5) Screws
from each End Panel and
Strapping
End Panel
remove the panels from the
cabinet. Cut the straps that
secure Biological Safety
Cabinet to the pallet.
Figure 3.1 Biological Safety Cabinet on pallet
Step 2 Unsecure Weight
Remove the (2) Pan Head
Screws and Nuts to remove the
Nut
AnglePan Head Screws
Angle that secures the Sash
Weight on the left end of the
BSC.
Figure 3.2 Sash Weight Lock-down
3.2
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 19
User and Operation Manual Chapter 3
Step 3 Level Sash
Ensure that the Sash Belt is
Sash Belt Set Screw
Pully
engaged with the Pulley on
both ends, ensuring sash looks
level horizontally. If sash is not
level, loosen Set Screw on one
pulley, leaving the other pulley
xed, and slowly move the
sash up and down until level.
Figure 3.3 Sash Belt Pully
Step 4 Set Cabinet
Setting the Kewaunee
Biological Safety Cabinet
will require a oor jack and
multiple personnel. Lift the
BSC with the oor jack and
slide onto the stand, aligning
the holes on the stand with the
threaded connectors on the
bottom frame of the cabinet.
Insert (4) 5/16" Bolts with
Washers thru Frame into the
Cabinet and tighten securely.
Align holes on stand with
threaded connectors
on bottom of Biological
Safety Cabinet and insert
bolts
Figure 3.4 Attachment of Cabinet to Stand
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
3.3
Page 20
Chapter 3 User and Operation Manual
Step 5 Attach Drain Valve
Pipe Fitting
Figure 3.5 Drain Valve Parts
Drain Plate
Ball Valve
Drain Pan
1. Remove the cabinet
Worksurface by lifting the
two Handles at the front of
the Worksurface.
2. Remove and discard the Plug
Button that seals the drain
mounting hole (front right of
pan) and clean the surface
of any remaining sealant.
(putty knife works well)
3. Assemble Drain Valve Assembly
as shown in Figure 3.5 using
plumbers tape.
4. Apply a light bead of silicone
sealant to the mounting
surface of the Drain
Assembly and attach the
assembly to the bottom of
the cabinet using four (4)
1
/4"-20 Hex Nuts as shown
in Figure 3.6
Silicone Sealant
Valve Assembly
Figure 3.6 Drain Valve Assembly Installation
i Attention
¡
Drain Valve is not installed at factory to facilitate shipping. It is packed in the
Loose Parts box inside the cabinet and must be properly installed before
cabinet is placed into operation.
Hex Nuts (4)
5. Tighten nuts evenly until
assembly is fully seated
and sealant begins to seep
from around all edges of the
mounting plate.
6. Wipe away excess sealant
from the area and verify
that the drain hole is
unobstructed and clean.
7. Verify that the drain valve is
in the closed position.
8. Reinstall the Worksurface
and allow the silicone
sealant to cure for at least
eight hours before exposing
the Drain Pan to liquids.
3.4
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 21
User and Operation Manual Chapter 3
Step 6 Attach Armrest
End Cap
Armrest Tube
BSC Stand Top Rail
Armrest Bracket
Figure 3.7 Armrest Assembly
Self-drilling Screw
1. Attach the three (3) Armrest
Hex Nut & Washer
Bracket to the BSC Stand using
the supplied
Washers.
2. Insert the armrest End Caps into
each end of the Armrest Tube.
3. Place the Armrest Tube on the
Armrest Brackets and center
horizontally.
4. Attach the tube to the brackets
using the supplied #8x
drilling Screws
Position Biological Safety Cabinet:
1
/4"-20 Hex Nuts and
1
/2" Self-
Position the Biological Safety Cabinet in an accessible location free of turbulence as
described in Chapter 2. Be sure hood is not located under a make-up air diffuser
of any kind. Also, avoid placing the unit next to any frequently used door or high
trafc areas. Be sure a properly sized exhaust duct is located above the cabinet.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
3.5
Page 22
Chapter 3 User and Operation Manual
Service Feed Requirements
Mechanical connections, when required, should be made after the cabinet is in
position. The tting stub-outs are located on the lower front of the cabinet’s right and
left side panels. See Figure 3.8 and Figure 3.9 below.
1. The use of ammable gas services is not recommended.
2. Any service used should be at a line pressure of 75 psi or less.
3. Active high velocity gas jets inside the Biological Safety Cabinet should be
avoided due to the turbulence they create.
4. Service lines should be connected by a licensed professional
Alternate
Service Location
Service Stub-out
Figure 3.8 Fixture stub-outs behind removed end panel Figure 3.9 Mechanical Fixtures inside cabinet
Alternate
Service Location
Service Fitting
3.6
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 23
User and Operation Manual Chapter 3
Electrical Power Connection
The Interceptor® has one power cord which is hardwired to the cabinet. The cord
should be plugged into a 120 VAC, 20 amp, grounded, electric outlet. Always use
waste-high or oor-positioned outlets to facilitate disconnection in an emergency. The
powered stand, if ordered, takes an additional 8.6 amps at 120 VAC off its own power
cord and should also be plugged into a waste-high or oor-positioned outlet.
! WARNING
ê
The electrical outlets inside the Interceptor® are grounded. This is particularly
important since all internal surfaces are stainless steel and conduct electricity.
Under NO CIRCUMSTANCES use ungrounded plugs in these outlets.
As soon as the cabinet power cord is connected, you are ready to follow the “Startup”
procedure on page 4.2. The electrical cord must not be unpluged until cabinet
fan and lights are turned off. The cabinet should be disconnected from the main
voltage by unplugging the cord to remove power. For electrically-powered lift stands,
disconnection is also accomplished by unplugging the power cord.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
3.7
Page 24
Chapter 3 User and Operation Manual
Exhaust Connection
The Class II B2 Interceptor has a hard duct connection. The photo below shows how
this connection is made to the building exhaust fan. The fan inside a B2 unit only
provides internal downow air. Exhaust is entirely accomplished with a building
exhaust fan.
Figure 3.9 Class II B2 Interceptor Duct Connection
Exhaust requirements
The building ventilation system must be veried that it can handle the required
exhaust volumes of the cabinet before connection.
Table 3.3
! WARNING
ê
A B2 Biological Safety Cabinet may become dysfunctional if the ventilation
system fails. Check building ventilation system before using cabinet!
Size of Biological
Safety Cabinet
1400mm 755
2000mm 1120
Biological Safety Cabinet
Exhaust CFM
3.8
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 25
User and Operation Manual Chapter 4
CHAPTER 4
Cabinet Operation
Theory of Operation
The Interceptor® is a Class II, B2 Biological Safety Cabinet. Its function is to
isolate bacterial samples from cross-contamination and protect the user and
the environment around the cabinet from being contaminated by biological or
particulate material inside the cabinet.
It accomplishes this objective by bathing biological samples in HEPA ltered clean
air while keeping contaminants contained by having a continuous substantial inow
of air through the 8" cabinet sash opening.
The BSC will safely operate at a temperature range of 55ºF to 85ºF (13ºC - 29ºC) and
a relative humidity of 10% to 70%.
Class II, B2 Biological Safety Cabinets are made to safely contain bacterial samples
that fall into the Biological Safety levels 1, 2, 3, and 4 if accompanied by level
appropriate protection garments (see Protective Clothing on page 6.1). The following
is a description of each level as published in the CDC BMBL 5th edition:
“Biological Safety level 1 (BSL-1) is the basic level of protection and is
appropriate for agents that are not known to cause disease in normal,
healthy humans. Biological Safety level 2 (BSL-2) is appropriate for
handling moderate-risk agents that cause human disease of varying
severity by ingestion or through percutaneous or mucous membrane
exposure. Biological Safety level 3 (BSL-3) is appropriate for agents with
a known potential for aerosol transmission, for agents that may cause
serious and potentially lethal infections and that are indigenous or
exotic in origin. Exotic agents that pose a high individual risk of lifethreatening disease by infectious aerosols and for which no treatment
is available are restricted to high containment laboratories that meet
biosaftey level 4 (BSL-4) standards.”
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
4.1
Page 26
Chapter 4 User and Operation Manual
Startup Procedure
Raise the vertical sash to the 8" operating line. Push the Menu/OK button on Control
Panel Touch Pad (Figure 4.1) while at Startup/Home Screen (Figure 4.2), then press
®
the Fan button once and the fan motor will start. The Interceptor
all dust, bacteria, and viral matter and delivering HEPA ltered, clean air to the
Biological Safety Cabinet work zone. The air ow pattern is shown in Figure 4.3 on
following page.
is now intercepting
Figure 4.1 Control
Panel Touch Pad
! WARNING
ê
Class II B2 Biological Safety cabinets must be certified before initial use, after
being moved, and after any service, including required annual recertification.
Service must be performed by an NSF accredited certifier using NSF/ ANSI 49
criteria and should include, at minimum, the following test:
• Downflow Velocity Profile Test
• Inflow Velocity Test
• Airflow Smoke Pattern Test
• HEPA Filter Leak Test
the
®
Interceptor
OK to Run, Down for Help, Up for Configuration
version C10102H40011
Figure 4.2 Monitor Startup/Home Screen
4.2
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 27
User and Operation Manual Chapter 4
Interceptor Class II, B2 Air Flow Patterns
It is recommended that the cabinet fan be turned on and allowed to operate for
several minutes removing any suspended particulates. Cabinet interior should then
be wiped down with 70% ethanol (EtOH), or other approved disinfectant.
HEPA
Filtered
Air
Room Air
Contaminated
Air
HEPA
Filtered Air
Figure 4.3 B2 Interceptor Biological Safety Cabinet Air Flow Patterns
Room Air
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
4.3
Page 28
Chapter 4 User and Operation Manual
Operating the Interceptor® BSC
When the Interceptor® cabinet is plugged in, the Control Monitor displays the
Startup/Home Screen, Figure 4.4. Press the Menu/OK button on the Control Panel
(Figure 4.5) to take you to the Run Screen. Pressing
Conguration Menu; pressing
instructional slide show.
(down arrow button) selects Help, which displays an
(up arrow button) displays the
the
®
Interceptor
OK to Run, Down for Help, Up for Configuration
version C10102H40011
Figure 4.4 Control Monitor — Startup/Home Screen
When at the Run Screen, Press the Fan button on the Control Panel (Figure 4.5)
to begin the warm-up cycle. After 5 minutes, the Control Monitor should look like
Figure 4.6. The uorescent light is controled by the Light button and may be pressed
anytime after the fan is started.
Figure 4.6 Control Monitor — Run Screen after warm-up
Figure 4.5 BSC Control Panel
A brand new BSC is set for 100% lter capacity and 105 FPM. When face velocity
drops below 100 FPM, trained personnel should make adjustments to the system.
4.4
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 29
User and Operation Manual Chapter 4
Suggested or Recommended Workflow
Once skilled in your lab’s Standard Operating Procedure (SOP), don appropriate
protective garments and gear suitable to the level of safety required. Place items
for use in the procedure in the left section of the work area. Place equipment in
the center of the work area. Designate the right section of the BSC for waste to be
disposed of at the procedure’s end.
As you perform the procedure, waste materials will accumulate on the right side of
the cabinet.
After the procedure is nished, remove waste materials, disinfect as is customary at
your facility, and place materials away. Each time the unit is used, it should be left
clean. Follow your SOP for shut down or night setback procedures.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
4.5
Page 30
Chapter 4 User and Operation Manual
Control Monitor
The Control Monitor Run Screen can alert you to several possible problems as you
proceed:
1) Low inow velocity (Face Velocity).
2) Filter system full (Remaining Filter Capacity)
3) Check Sash Height. Always set the sash at the 8” line when conducting
experiments. This is the setting that has received NSF certication.
4) UV Light On
Figure 4.8 Control Monitor Run Screen — Warning – Sash not at 8"
UV Light
The UV germicidal disinfecting light may be used if one has been installed; simply
turn off the fan, fully close the sash, and turn on the light using the UV Light button.
Because UV rays are dangerous, the light will not operate unless the sash is fully
closed. Glass stops UV radiation. If the UV light is not turned off before the sash is
raised, the safety interlock will disable the UV light, but the fan will not operate until
the UV light is turned off at the Control Panel.
! WARNING
ê
If the UV option is on your BSC, be sure safety overrides are never
immobilized! UV light should NEVER be on while sash is open.
4.6
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 31
User and Operation Manual Chapter 4
Control Monitor Messages and Meanings
Variable Measurement Method Importantance Recommended Action
Airow
(optimal value
is 105 FPM)
Since the inow at any point of the
8" sash opening can vary widely,
it is the average inow (CFM/sq ft)
that is actually measured. Since
the “air in” through the 8" sash
opening and blower intake exactly
equals “air out”, the exhaust port,
the Interceptor
exhaust port velocity into FPM
inow velocity at the sash opening.
®
converts the FPM
Proper inow at 8"
assures containment of
contaminants within the
cabinet. NSF 49 requires
a minimum inow velocity
of 100 FPM.
The Run Screen shows
airow on the right pie
graph. Any velocity
between 100 FPM and
110 FPM is acceptable. If
face velocity falls below
100 FPM, discontinue work
and contact your Accredited
Technician for remediation.
Filter
Sash ≠ 8"
UV Light
(when installed)
The static pressure differential
between the negative and positive
pressure is proportional to lter
loading. It is measured by the
Pressure Sensor and converted by
the Contol Module to a lter loading
percentage.
A micro switch interaction with
the counterweight activates this
warning.
When the sash is closed and the
fan is off, the disinfecting UV Light
may be activated using the UV Light
button on the Control Panel Touch
Pad.
The Interceptor® exceeds
NSF requirements for
maximum static load.
The cabinet is designed
to be used at a 8" sash
opening. Any other
opening is inappropriate. If
the fan is on at any height
other than 8", the Check
Sash Height red warning
light displays.
Be sure UV Light Safety
Overrides are never
immobilized! UV light
should NEVER be on
while sash is open.
An Accredited Technician
may need to adjust fan
speed to maintain face
velocity or change lter.
Return the sash to 8" or
turn the unit off and close
the sash.
Close sash and turn fan off
to reactivate UV.
UV Hours
Remaining
Table 4.1
Internal countdown timer. Setting
of 2000 hours can be reset to
manufacturers recommendations.
When the hood UV light is
on, it is important to know
how much life is remaining
in the UV lamp before its
UV output diminishes.
The “hours remaining”
notation will show in the
middle column while this
information screen is
showing.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Replace UV lamp, even if
it still glows, when ‘0’ hours
remaining is indicated.
4.7
Page 32
Chapter 4 User and Operation Manual
Touch Pad Control Functions
Function Button Operations
Menu/OK
(Select)
▲
▼
UV Light Button
Alarm Mute
Fan
Light
Table 4.2
Monitor Run Screen
1. Access Menu
2. Enter Data
3. End routine
1. Increase programmed value
2. Access menu
3. Increment up on menu
1. Decrease programmed value
2. Access Instructional Video
3. Increment down on menu
Activates UV light ONLY
when sash is closed and fan is off
Mutes audible alarm
Turns BSC air fan on and off
Turns work light on and off when the sash is open
Pie Chart Display Explanation
Remaining Filter Capacity
Face Velocity
Table 4.3
Figure 4.9 BSC Control Screen
Shows remaining lter capacity
Shows face velocity at 8” opening
(100 FPM to 110 FPM is optimal)
4.8
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 33
User and Operation Manual Chapter 4
Monitor Screen Displays
The Kewaunee Interceptor® Biological Safety Cabinet employs a sophisticated
control system using a built in color VGA screen to display menus and options. The
following pages illustrate the different states of the monitor with illustrations of the
various screens and schematics of each of the menus. The schematic charts show
all options for each menu item as well as the factory setting. Please note that it is
recommened that menu items highlighted with an asterisk and shaded in grey be
changed only by a factory authorized technician.
The menus and options are controlled by the BSC Touch Pad Control located at the
bottom of the right facia post and shown on page 4.4, Figure 4.5.
the
Interceptor
OK to Run, Down for Help, Up for Configuration
version C10102H40011
Figure 4.10
Startup/Home Screen
displays when cabinet is plugged in
®
Press OK to Run
Press Up Arrow for Configuration
Press Down Arrow for Help
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
4.9
Page 34
Chapter 4 User and Operation Manual
Monitor Screen Displays
Run Screen
Fan OFF
displays when Fan is Off
Press Fan Switch to Start
Press Menu/OK for 5 seconds
to return to Startup Screen
Figure 4.11
Figure 4.12
Run Screen
Fan Warmup
displays when Fan is On
during warmup period
Press Menu/OK for 5 seconds
to return to Startup Screen
Run Screen
Fan On
displays when Fan is On
after warm up period
Figure 4.13
4.10
Press Menu/OK for 5 seconds
to return to Startup Screen
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 35
User and Operation Manual Chapter 4
Monitor Screen Displays
Configuration Setup Menu
Main Menu
Press Up or Down Arrow to select
option, then Press OK
See Page 4.13
Figure 4.14
Configuration Menu
Figure 4.15
Use to set Calibration, Filter, and
UV Light Options
Press Up or Down Arrow to select
option, then Press OK
See Page 4.14
Calibration
Configuration Menu
Use to set Calibration Options
Press Up or Down Arrow to select
option, then Press OK
See Page 4.15
Figure 4.16
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
4.11
Page 36
Chapter 4 User and Operation Manual
Monitor Screen Displays
Calibration Screen
Use to Calibrate Cabinet at time of
installation and after filter changes
Enter Password, then follow screen
prompts
Note: To Be Performed by Certified
Technician Only
see Page 4.20
Figure 4.14
Password Menu
Figure 4.15
Use to change Passwords
Press Up or Down Arrow to select
option, then Press OK
see Page 4.13
Password Screen
Used to enter Configure and Password
Menus and change Passwords
Press Up or Down Arrow to select digit
Press OK to advance to next digit
Press OK with complete
Figure 4.16
4.12
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 37
User and Operation Manual Chapter 4
Monitor Menus
Setup Menu Schematic
Menu Item
Configure to Configuration Menu See Page 4.14
Calibration to Calibration Steps See Page 4.20
Password to Password Menu
Exit return to Startup Screen
Table 4.4
See Page 4.13
below
Password Menu Schematic
Input Screen Title Input Option Factory Setting
Password Enter Password: XXXX
Menu Item Input Screen Title Input Option Factory Setting
Admin Enter Password: XXXX
New Password: XXXX
Confirm New Password: XXXX
contact
Kewaunee
contact
Kewaunee
Configure New Password: XXXX 9999
Confirm New Password: XXXX
Calibration New Password: XXXX 9999
Confirm New Password: XXXX
Exit return to Setup Menu
Table 4.5
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
4.13
Page 38
Chapter 4 User and Operation Manual
Conguration Menu Schematic
Input Screen Title Input Option Factory Setting
Password Enter Password: XXXX 9999
Menu Item Input Screen Title Input Option Factory Setting
Cal config menu to Calibration Configuration Menu
Input 1 * Input 1 Activation:
Input 3 * Input 3 Activation:
Relay Output 1 * Relay 1 Activation:
Relay Output 2 * Relay 2 Activation:
Relay Output 3 * Relay 3 Activation:
Pushbutton 1 * PB 1 Activation:
Pushbutton 2 * PB 2 Activation:
Pushbutton 3 * PB 3 Activation:
open contact
close contact
open contact
close contact
open contact
close contact
open contact
close contact
open contact
close contact
Not Enabled
Enabled
Not Enabled
Enabled
Not Enabled
Enabled
close contact
close contact
close contact
close contact
close contact
Enabled
Enabled
Enabled
see page
4.15
see page
4.16
see page
4.17
see page
4.17
Sash = 10 inches * Sash High Alarm:
if On
Sash High
Timer:
Repeat Timer Off
Repeat Timer On
Xmin 5min
Modbus Settings * to Modbus Settings
Sensor Err Options * to Sensor Err Options
Filter Options to Filter Menu
UV Light Options to UV Light Menu
Exit return to Setup Menu
Table 4.6
4.14
* Should only be changed by Factory Authorized Technician
Interceptor Biological Safety Cabinet Class II B2 120 Volt
Repeat Timer On
see page
4.18
see page
4.18
see page
4.19
see page
4.19
July 2014
Page 39
User and Operation Manual Chapter 4
Conguration Menu > Calibration Conguration Menu Schematic
Menu Item Input Screen Title Input Option
Pressure Calib Delay Pressure Calibration Delay: Xsec
Low Air Alarm Low Air Alarm: XXfpm
Low Air Cutoff Low Air Cutoff:
if ON
Low Air Cutoff: XXfpm
OFF
ON
Warning Air Alarm Warning Air Alarm: XXfpm
Warning Air Reset: Xfpm
High Air Alarm High Air Alarm:
if ON
High Air Alarm: XXXfpm
OFF
ON
Low Air Fluc * Low Air Fluc: XX%
Factory Setting
5sec
89fpm
OFF
99fpm
4fpm
OFF
150fpm
5%
High Air Fluc * High Air Fluc: XX%
Low High Diff Low High Air Diff: XXfpm
Warning-Alarm Time Warning-Alarm Time: Xsec
Alarm-Warning Time Alarm-Warning Time: Xsec
Show Air Flow Show Airflow:
Show Time Line Show Time Line:
Audible Alarm Audible Alarm:
OFF
ON
OFF
ON
Not Enabled
Enabled
Sensor Difference Sensor Difference: XX%
Sensitivity Sensitivity: XX%
10%
30fpm
5sec
1sec
Inactive
For future use
Inactive
For future use
Enabled
10%
100%
Fan Run Up Timer Fan Run Up Timer Duration: Xmin
Exit return to Configuration Menu
Table 4.7
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
5min
4.15
Page 40
Chapter 4 User and Operation Manual
Conguration Menu > Pushbutton 1 Options Schematic
Menu Item Input Screen Title Input Option Factory Setting
None
Select O/P Relay * PB 1 Relay:
Interlock * PB 1 Interlock:
Output 1
Output 2
Output 3
Not Active
Off enable Pb 2
On enable Pb 2
On RU enable Pb2
Output 1
Not Active
PB 1 Run Up Timer:
Not Enabled
Enabled
None
Run Up Timer *
if
Enabled
PB 1 Run Up
Timer Relay:
Output 1
Output 2
Output 3
PB 1 Run Up Time: XXsec
PB 1 Run Down Timer:
Not Enabled
Enabled
Run Down Timer *
if
Enabled
PB 1 Run Down
Time:
XXsec
Fan On/Off
Icon display * PB 1 Icon:
Set Back OR
Not Enabled
Sticky Button * PB 1 Sticky:
Not Enabled
Enabled
Exit return to Configuration Menu
Table 4.8
Not Enabled
Not Enabled
Fan On/Off
Not Enabled
4.16
* Should only be changed by Factory Authorized Technician
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 41
User and Operation Manual Chapter 4
Conguration Menu > Pushbutton 2 Options Schematic
Menu Item Input Screen Title Input Option Factory Setting
None
Select O/P Relay * PB 2 Relay:
Icon display * PB 2 Icon:
Output 1
Output 2
Output 3
Not enabled
Pump
Lights
Services
UV Lights
Output 2
UV Lights
Sticky Button * PB 2 Sticky:
Exit return to Configuration Menu
Table 4.9
* Should only be changed by Factory Authorized Technician
Not Enabled
Enabled
Not Enabled
Conguration Menu > Pushbutton 3 Options Schematic
Menu Item Input Screen Title Input Option Factory Setting
None
Select O/P Relay * PB 3 Relay:
Icon display * PB 3 Icon:
Output 1
Output 2
Output 3
Not enabled
Scrubber On/Off
Purge On/Off
VAV Min/Norm/Max
VAV Min/Norm
Output 3
Scrubber On/Off
Sticky Button * PB 3 Sticky:
Exit return to Configuration Menu
Table 4.10
* Should only be changed by Factory Authorized Technician
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Not Enabled
Enabled
Not Enabled
4.17
Page 42
Chapter 4 User and Operation Manual
Conguration Menu > Modbus Setting Schematic
Menu Item Input Screen Title Input Option Factory Setting
Slave ID * Slave ID: X 1
1200
2400
Baud Rate * Baud Rate:
Parity Type * Parity Type:
Exit return to Configuration Menu
Table 4.11
* Should only be changed by Factory Authorized Technician
4800
9600
14400
19200
None
Odd
Even
9600
None
Conguration Menu > Sensor Err Options Schematic
Menu Item Input Screen Title Input Option Factory Setting
Buzzer On/Off * Sensor Error Buzzer:
Exit return to Configuration Menu
Table 4.12
* Should only be changed by Factory Authorized Technician
Off
On
On
4.18
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 43
User and Operation Manual Chapter 4
Conguration Menu > Filter Menu Schematic
Menu Item Input Screen Title Input Option Factory Setting
Clean Pressure Clean Pressure: X.XX ins wg 0.62 ins wg
Dirty Pressure Dirty Pressure: X.XX ins wg 1.86 ins wg
Warning % Filter Warning: XX% 49%
Alarm % Filter Alarm: XX% 30%
Pressure Fluc Pressure Fluc: XX% 25%
Pressure Filter
Low Pressure Alarm Low Pressure Alarm:
Low Pressure Alarm
Setpoint
Exit return to Configuration Menu
Table 4.13
Pressure Averaging
Period:
Low Pressure
Alarm Setpoint:
Xsec 1sec
Not Enabled
Enabled
XX% 30%
Not Enabled
Conguration Menu > UV Lights Menu Schematic
Menu Item Input Screen Title Input Option Factory Setting
UV Lifetime UV Lifetime: XXXXhrs 2000hrs
Reset UV Hours Confirm Reset
UV Time Duration UV Timer Duration: XXmin 33min
Exit return to Configuration Menu
Table 4.14
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
This cannot be
undone!
4.19
Page 44
Chapter 4 User and Operation Manual
Calibration — Should only be performed by Certied Technician
Calibration step possible error message
Enter Password XXXX
Airflow sensor test checking OK
input screen/progress status
Use arrow keys to set
Press OK to go to next digit
Sensor error
Switch Fan On Press Fan Button OK
Set fan voltage to 70% of run value OK
Set sash to 10” and enter measured face velocity XXfpm OK
Use arrow keys to set measured value
Low airflow calibration Calibrating, please wait OK
Deviation too high
Set fan voltage to run value and enter measured velocity XXfpm OK
Press OK to repeat
Up Arrow to Quit
Press Fan Button
Adjust fan voltage
Press OK to Continue
Press OK to Continue
Press OK to repeat
Up Arrow to Quite
Increase face velocity
High airlow calibration Calibrating please wait OK
High and Low samples too close
Deviation too high
Turn Fan Off Press Fan Button OK
Pressure Calibration Sampling pressure XX%... OK
Calibration complete OK
Table 4.15
4.20
Interceptor Biological Safety Cabinet Class II B2 120 Volt
Use arrow keys to set desired value
Press OK to Continue
Press OK to repeat
Up Arrow to Quit
Press Fan Button
July 2014
Page 45
User and Operation Manual Chapter 5
CHAPTER 5
Care and Maintenance
This chapter details maintenance that can be undertaken by laboratory personnel,
supervisors; and/or Accredited Technicians.
Access to Maintenance Areas
Do not remove the Air Chamber Access Panel or Blower Access Panel shown in
Figure 5.1. The area behind these panels is contaminated. Contact your Accredited
Technician for maintenance of these areas.
Air Chamber Access Panel
Figure 5.1
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
5.1
Page 46
Chapter 5 User and Operation Manual
Frequently Required Maintenance Procedures
Before following any of these procedures, be sure all in-house safety precautions
are followed regarding personal contamination. Face masks and wearing two pair of
gloves are recommended in all cases.
Item Procedure
Filter replacement
Refer to Maintenance & Technical Manual
Cabinet interior cleaning
NOTE: DO NOT USE
CHLORINE BLEACH
Clean cabinet exterior
Check service ttings
Check remaining life of
UV Lamp
Recertify cabinet
Cleaning Towel Catch
(only perform this if face
velocity drops or towels are
known to have been drawn
into the back bafe)
Removal and cleaning of
stainless steel front grille
Removal of worktop
Table 5.1
a) Use UV disinfecting light for at least 30 minutes or time recommended by
your Standard Operating Procedure (SOP) if UV light is installed.
b) Open sash and using disinfectant approved for use in your procedures,
carefully clean worktop, top and bottom. Lift top and disinfect drain pan.
Use mild soap and approved disinfectant
Visual test with soap solution, inspecting for bubbles indicating leaks.
View lamp life on Control Monitor
Use NSF 49 Accredited Technician
With worktop lifted, reach below rear bafe slot and remove debris from the
towel catch grille behind the rear bafe.
NOTE: this procedure becomes easier if worktop can be removed entirely from
the cabinet. Since the top may be partially contaminated be sure removal and
temporary storage of the top is undertaken using procedures approved by your
facility’s Health and Safety Department.
Remove fasteners from the grille, then lift and remove. Clean grille with
approved disinfectant and replace.
Usually considered part of interior disinfection procedure; be sure underside
of top is disinfected before removing from the unit. Clean drain tray thoroughly
using approved disinfectant before returning the worktop to operating position.
Infrequently Required Maintenance Procedures
Item When Required Procedure
Hinged top front
panel operation
Changing lamps
Resetting circuit
breakers
Table 5.2
5.2
Needed only for uorescent light
replacement. All other areas behind
panel should be accessed only by
Accredited Technician.
Fluorescent lights may be operated
until they burn out. UV lights should
be replaced when their useful life is
exceeded.
When:
a) outlets are not working
b) fan and control unit will not
power up
Interceptor Biological Safety Cabinet Class II B2 120 Volt
Remove the small screws at each end of
the bottom edge of the panel. Panel will lift
assisted by two gas struts.
Disconnect the power cords. Remove pinned
UV or uorescent lamp by turning lamp 90°
and pulling forward. New lamps are installed
by reversing this procedure.
Resetting these requires disconnecting power
and removing the cover on the electrical
junction box under the front panel of the BSC.
Reset the two breakers inside the box, then
plug the unit back in.
If breakers trip, call an Accredited Technician or
trained personnel. If breakers do not trip, remove
wall plug reattach lid, and reconnect power.
July 2014
Page 47
User and Operation Manual Chapter 5
Maintenance Log
We recommend the formation of a logbook to record recommended maintenance
activities as the example shown below:
Month Clean Exterior Check all Service Fittings
January
February
March
April
May
June
July
August
September
October
November
December
Daily Basis:
Disinfect surface work areas, before and after each use
Weekly Basis:
Surface disinfrect drain pan
Quarterly Basis:
Check lights for proper function
Check for malfunctions
Spot clean stainless steel surfaces
Annual Basis:
Inspect and Recertication by an Accredited Technician
Table 5.3
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
5.3
Page 48
Chapter 5 User and Operation Manual
Disinfection
It is recommended that your Biological Safety Cabinet be thoroughly disinfected
before and after each use. This procedure is to destroy any contaminates that may
remain within the cabinet and may compromise your work. This should be at least
part of your Standard Operating Procedure (SOP) but not a substitution for it. The
entire inside of the cabinet should be wiped down during the disinfection. This
includes the side and rear walls, the removable worksurface, both top and underside
(as needed) and the drain pan.
It is recommended that the cabinet fan be turned on before disinfection procedure
and allowed to operate for several minutes to remove any suspended particulates.
Cabinet interior should then be wiped down with 70% alcohol, or other approved
disinfectant. After cabinet has been thoroughly disinfected, then it is very important
to rinse the surfaces with sterile water to remove any residue.
Storage
If the Interceptor® is to be decommissioned or stored for protracted periods, consult
with your health and safety personnel for appropriate procedure.
If the lters were not full the last time the cabinet was used, all contamination will be
sealed inside the fan compartment and positive plenum, and therefore isolated.
The storage mode for the unit will be inuenced by at least the following issues:
1. Virulence or toxicity of materials used in the cabinet
2. Resistance of materials to UV light or other disinfection procedures used on the
cabinet interior
3. Length of time item is to be stored before reuse
Filters
Filter replacement must be completed by an NSF Accredited Technician. See
Maintenance and Technical Manual.
Lamps
See Table 5.2 for information on replacement of uorescent and/or UV lamps. See
Table 7.2 for replacement lamp part numbers.
5.4
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 49
User and Operation Manual Chapter 5
Decontamination
Decontamination must be undertaken by an NSF Accredited Technician. Details
®
on decontamination and lter change-out procedure can be found in the Interceptor
Maintenance and Technical Manual.
! WARNING
ê
Decontamination may only be performed by an NSF Accredited
Technician.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
5.5
Page 50
Chapter 5 User and Operation Manual
5.6
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 51
User and Operation Manual Chapter 6
CHAPTER 6
Operator and Product Protection
Safe Operation Guide
The Biological Safety Cabinet may only be used in the 8" sash open position due to
calibrated air ows. An opening other than 8" during a procedure will not protect the
operator from materials inside the hood.
Protective Clothing
The following are general guidelines only and should not replace any of your
laboratory’s Standard Operating Procedures (SOP).
Before beginning any work, the user should rst thoroughly wash their hands with a
germicidal soap.
Don appropriate personal protective equipment for the bio safety level and work being
performed. Operations being performed in BSL level 1 suggest wearing protective
laboratory coats, gowns or uniforms to prevent contamination of personal clothing.
Protective eyewear is also suggested.
BSL level 2 and 3 suggests wearing gloves, protective laboratory coats, gowns,
smocks or uniforms (possibly tie back, wrap around gowns or scrub suits). Eye and
face protection include goggles, mask, face shield or other splatter guard are also
suggested. Eye, face and Respiratory protection must be worn in rooms containing
infected animals.
All procedures being performed in a BSL Level 4 and using a class II BSC require the
user to wear a one piece air supplied positive protection suit.
UV Lamp
The Control Monitor Run Screen will alert the operator when the UV lamp should
be replaced. The lamp may still illuminate at this point; however, UV output will be
greatly diminished.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
6.1
Page 52
Chapter 6 User and Operation Manual
HEPA Filters
HEPA lters are the most important safeguard of a Biological Safety Cabinet. The
HEPA lter has a proven efciency of 99.99% at 0.3 microns. The HEPA lter is made
from a single sheet of borosilicate bers that have been treated with a wet-strength
water–repellant binder. The lter is pleated to increase overall surface inside the
lter frame and is protected by corrugated aluminum separators. These separators
protect the pleats from collapsing and provide a path for airow. Although aluminum
separators are most common, there are other materials that are acceptable. The
lters are then glued to a wood, metal or plastic frame. The lters are very fragile and
careless handling can easily compromise their integrity. It is important that the lters
be leak tested when initially installed and whenever the cabinet is moved or relocated.
Note that much of this information is credited to CDC BMBL 5th Edition.
HEPA lters are only efcient on particulates; they are not designed to lter gases.
It is important to check the lters on a regular basis (at least annually during
recertication). As the lters collect particulates, it will become increasingly harder
to maintain the airow balance within the cabinet. Filters should be changed at
recommended intervals to ensure proper airow is being maintained within the
cabinets.
6.2
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 53
User and Operation Manual Chapter 7
CHAPTER 7
Troubleshooting
Problem Possible Cause Recommended Fix
No lights or
fan
No fans
Fluorescent
light not
working
UV light does
not illuminate
Reduced face
velocity
Contamination
of work inside
the cabinet
a. Unit unplugged Plug it in
b. BSC breaker open Reset breaker
c. Building breaker open Check outlet breaker
a. Sash is fully closed Open sash to 8"
b. Fan breaker/overload tripped Overload will reset if unit disconnected. If condition
persists, motor may need to be replaced. Contact
Kewaunee Scientic Corporation for assistance.
c. Sash-activated fan kill relay broken Replace
a. Lamp burnt out (look for dark
rings at opposite ends of glass
uorescent tube)
b. Lamp wiring defective Inspect and repair
c. Bad lamp ballast (symptom is
intermittent light)
a. Sash must be closed for UV light to
work (regular glass blocks UV rays)
b. Lamp burnt out Replace lamp
c. Lamp wiring/microswitch defective Inspect and repair
d. Bad lamp ballast (symptom is
intermittent light)
a. HEPA lter loaded Increase motor RPM or replace lter if 105 fpm
b. Towels have clogged towel screen
or visible bafe louvers
c. Exhaust outlet clogged with debris Clear outlet
a. Loaded downow lter Replace lters
b. Torn downow lter Replace lters
c. Cabinet inow not being captured
by front grill
d. Room turbulence Decrease turbulence or move cabinet
e. Cabinet balance has been disrupted Rebalance cabinet (by NSF Accredited Technician)
Replace lamps
Replace ballast located inside rooftop circuit box
Close sash
Replace ballast located in electrical box
face velicity is no longer achievable
Clean towel screen
(see Chapter 5 User & Operation Manual)
Remove grill, inspect, repair
Technical Support
Table7.1
To validate your warranty, please take a moment to register your Biological Safety
Cabinet. Registering your cabinet will allow us to provide you with maintenance
information and product updates.
To register, ll out the registration form at the end of this section and mail or fax to
the address listed at the bottom of the form. If assistance is necessary, please contact
us at 704-873-7202.
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
7.1
Page 54
Chapter 7 User and Operation Manual
Detailed View of Cabinet Parts
Blower Intake Hood
Exhaust Thimble
Front Panel
Shaft Guard
LCD Control
Exhaust Filter
Lights/Bracket
Sash Weight
Channel for
Micro-switch
End Panel
Air Intake Grill
Worksurface
Sash Clip
Sash
Facia Front
Facia Back
Left Facia Decal
Figure 7.1
7.2
Facia Track
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 55
User and Operation Manual Chapter 7
Replaceable Parts List
Part Numbers
Parts INT-1400B INT-2000B
30/40 μF Motor Capacitor F-7262-05 F-7262-00
Micro Switch F-7242-00 F-7242-00
Fluorescent / UV Relay F-7232-00 F-7232-00
10 Amp Circuit Breaker F-7240-04 F-7240-04
20 Amp Circuit Breaker F-7240-00 F-7240-00
Motor Solid State Relay F-7233-00 F-7233-00
Potentiometer F-7239-00 F-7239-00
Variable AC Power Control F-7238-00 F-7238-00
UV Lamp F-7249-00 F-7249-00
T5 Lamp F-6347-46 F-7248-00
Removable Work Tray 050007-48 050007-72
Tray Handle F-7209-00 F-7209-00
Bottom Intake Grille 050008-48 050008-72
Sash Glass with Handles Ass’y 050009-48-FIN 050009-72-FIN
Electric Junction Box 050031-00 050031-00
Exhaust HEPA filter F-7275-48-B F-7275-72-B
Supply HEPA filter F-7276-48 F-7276-72
Removable Work Tray Support 050063-00 050063-00
Sash Sweeper Plate 050076-48 050076-72
Top Exhaust Enclosure 050142-48W-B 050142-72W-B
12" Air Tight Damper INT-DAMPER INT-DAMPER
Downflow Perforated Grille 050079-48 050079-72
Arm Rest Bracket 050195-0A 050195-0A
Arm Rest Plastic End Cap F-7279-00 F-7279-00
Aluminum Arm Rest F-7280-48 F-7280-72
Drain Valve Plate 050110-00 050110-00
316 SS Pipe Fitting F-7211-11 F-7211-11
316 SS Ball Valve F-7211-00 F-7211-00
Safety Labels 120 VAC F-8200-00 F-8200-00
Pressure Transducer F-7225-06 F-7225-06
Velocity Sensor F-7225-05 F-7225-05
Fan Assembly F-7247-01-D F-7247-00-D
Gas Spring Assembly F-7224-00 F-7223-00
Belt Drive to Counterweight F-6343-00 F-6343-00
Table 7.2
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
7.3
Page 56
Chapter 7 User and Operation Manual
Labels
The following depictions are replicas of the exact labels used within/on the Biological
Safety Cabinet and are generally self-explanatory.
Label Images Description Label Images Description
Airow
Sensor
Auxiliary
Power Inlets
Cabinet
Power Inlets
Drain
Valve
Kewaunee
Electrical
Diagram
Factory
Seal
Label
Factory
Test
Report
7.4
Universal
Biohazard
Warning
Filter
Scan
Electrical
Service
Caution
Electrical
Grounding
Label
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 57
User and Operation Manual Chapter 7
Label Images Description Label Images Description
Made
in
America
Quality
Control
Junction
Box Cover
Exhaust
Damper
Warning
Cabinet
Certication
Wiring
Serial
Number
and
Manufacture
Date
Sampling
Port
Speed
Control
Biohazard
Access
Panel
Warning
Electrical
Warning
Ultraviolet
Light
Warning
Label
Typical
Product
Label
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
7.5
Page 58
Chapter 7 User and Operation Manual
CLASS II B2 WIRING DIAGRAM - 1400B
Wiring Schematic
Circuit
(outlets)
B
G
2
B
W
W
10 Amp
B=0
Cable
20
W=10
G
A
1
B
R=24
B W G
B
B
W
DESCRIPTION
Black 12, 14, 16, or 18 AGW
White 12, 14, 16, or 18 AGW
B
T5 Ballast & Light
W
WIRE
COLOR
WIRE ID
G
W
G
W
Green 12, 14, 16, or 18 AGW
G
Red 12, 14, 16, or 18 AGW
R
J.B.
Primary
Green 23 AGW Primus Cable CAT6
Green/White 23 AGW Primus Cable CAT6
Blue 23 AGW Primus Cable CAT6
Blue/White 23 AGW Primus Cable CAT6
G
G/W
Bl
Bl/W
Jumper to
Sash Rod Bracket
Jumper to
Enclosure Cover
Ground Bar
Circuit
Brown 23 AGW Primus Cable CAT6
Brown/White 23 AGW Primus Cable CAT6
Br
Br/W
O
Jumper to
Ground Screw
Front Panel
G
Orange 23 AGW Primus Cable CAT6
Orange/White 23 AGW Primus Cable CAT6
O/W
Ground Screw
W
B
5
B
R = UV
- G/W
4
R = T5
Signal
(optional)
3
R = Ext. Fan
2
R = LO Alarm
R
1
R = Int. Fan
14 13
G
14 13
+ O
-Br
BB
14 13
+ Br/W
14 13
+ O/W
14 13
B
8 5
4 1
B
8 5
4 1
- Br
+ O
B
8 5
4 1
Signal Out To
Terminal Block
+ Br/W
8 5
4 1
8 5
4 1
B
12 9
B
B
12 9
B
12 9
Signal
In
To Terminal Block
G
R
12 9
12 9
B W G
UV Ballast & Light
- G/W
GFI Receptacle
B
Insert 120VAC wire in Heat Srink 6" Minimum from Relays
B
Cable
0"
Sash
SW
NO
R
B
C
RW
DPT
B
0 10 24
0
+
W
G
-
VEL
NO
G
Sash
SW
8" Open
C
W
Cable
0-10V
+
Relay
-
4PR 23 AGW
CAT6 600 MHZ
CMR ETL
Primus Cable UPT
+ O/W
- Br/W
33
+
+ O
Jumper
- G/W
32
-
G
31
+
- G/W
Jumper
30
-
29
+
+ Br/W
USB
24 VDC
28
-
- Br
SD
+
Keypad
Primus Cable UPT
100K
B
- G/W
- Bl/W
4PR 23 AGW
CAT6 600 MHZ
CMR ETL
(To Plenum)
(Pos. Outside)
LO
P Transducer
Vel. Probe
B1
B
W
30 MF
W
BR
G
G/Y
B
B
To Primary
unused
4
10 Amp
L3
2
CONTROLLER
MOTOR SPEED
B
HI
Cable
R
L1
W
G
Motor
Bl
Power Sup
+
-
24v
7.6
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
Page 59
User and Operation Manual Chapter 7
CLASS II B2 WIRING DIAGRAM - 2000B
Wiring Schematic
G
B
(outlets)
2
B
W
W
10 Amp
B=0
Cable
B W G
T5 Ballast & Light
G
A
20
1
B
B
B
W=10
R=24
W
W
W
DESCRIPTION
Black 12, 14, 16, or 18 AGW
White 12, 14, 16, or 18 AGW
B
W
WIRE
COLOR
WIRE ID
G
G
Green 12, 14, 16, or 18 AGW
G
Red 12, 14, 16, or 18 AGW
R
Green 23 AGW Primus Cable CAT6
G
J.B.
Primary
Green/White 23 AGW Primus Cable CAT6
Blue 23 AGW Primus Cable CAT6
Blue/White 23 AGW Primus Cable CAT6
Brown 23 AGW Primus Cable CAT6
Brown/White 23 AGW Primus Cable CAT6
G/W
Bl
Bl/W
Br
Br/W
Jumper to
Sash Rod Bracket
Ground Screw
Jumper to
Enclosure Cover
Ground Bar
Circuit
G
Orange 23 AGW Primus Cable CAT6
O
Jumper to
Ground Screw
Front Panel
Orange/White 23 AGW Primus Cable CAT6
O/W
W
B
5
B
R = UV
- G/W
4
R = T5
Signal
3
(optional)
R = Ext. Fan
2
R = LO Alarm
R
1
R = Int. Fan
14 13
G
14 13
+ O
-Br
BB
14 13
+ Br/W
14 13
+ O/W
14 13
B
8 5
4 1
B
8 5
4 1
- Br
+ O
B
8 5
4 1
Signal Out To
Terminal Block
+ Br/W
8 5
4 1
8 5
4 1
B
12 9
B
B
12 9
B
12 9
Signal
In
To Terminal Block
G
R
12 9
12 9
B W G
UV Ballast & Light
- G/W
Circuit
GFI Receptacle
B
Insert 120VAC wire in Heat Srink 6" Minimum from Relays
B
Cable
0"
Sash
SW
NO
RW
B
C
RW
Sash
SW
8" Open
C
NO
W
G
Cable
4PR 23 AGW
CAT6 600 MHZ
CMR ETL
Primus Cable UPT
+ O/W
DPT
B
0 10 24
0
+
G
-
VEL
0-10V
Relay
+
-
- Br/W
33
+
+ O
Jumper
- G/W
32
-
G
31
+
- G/W
Jumper
30
-
29
+
+ Br/W
USB
24 VDC
28
-
- Br
SD
+
Keypad
100K
B
- G/W
- Bl/W
AGW
T6 600 MHZ
unused
4
10 Amp
To Primary
Bl
L3
2
L1
CONTROLLER
MOTOR SPEED
W
B
Power Sup
+
24v
-
Cable
R
G
(To Plenum
HI
(Pos. Outside)
P Transducer
LO
Vel. Probe
B1
BR
Motor
B
W
40 MF
W
G
G/Y
B
B
Interceptor Biological Safety Cabinet Class II B2 120 Volt
July 2014
7.7
Page 60
WARRANTY
Biological Safety Cabinet Warranty
KEWAUNEE SCIENTIFIC CORPORATION warrants, for a period of three (3) years
beginning at the date of delivery, that this Biological Safety Cabinet shall be free
from defects in material and workmanship, excluding certain consumable items due
to normal wear and tear, i.e.; lters, UV lamps, uorescent lights, etc. KEWAUNEE
MAKES NO OTHER WARRANTY, EXPRESS OR IMPLIED, WRITTEN OR ORAL,
INCLUDING, BUT NOT LIMITED TO, THOSE OF MERCHANTABILITY OR FITNESS
FOR ANY PARTICULAR PURPOSE. Purchaser shall notify Kewaunee immediately
of any defective product. Kewaunee shall be given reasonable opportunity to
inspect the product prior to its return. No product shall be returned to Kewaunee
until receipt by purchaser of written shipping instructions from Kewaunee.
PURCHASER’S EXCLUSIVE REMEDY, AND KEWAUNEE’S SOLE LIABILITY,
SHALL BE, AT KEWAUNEE’S SOLE OPTION, REPAIR OR REPLACEMENT OF
THE NON-CONFORMING PRODUCTS OR THEIR PARTS, OR REFUND OF THE
PURCHASE PRICE. KEWAUNEE SHALL NOT BE LIABLE FOR ANY INCIDENTAL OR
CONSEQUENTIAL DAMAGES, LOSSES OR EXPENSES WHETHER INCURRED IN
CONNECTION WITH INJURY TO PERSONS OR PROPERTY.
Returned or Damaged Goods
Goods cannot be accepted without a Return Authorization ticket. Unauthorized
returns will not be accepted. Claims for cabinet(s) damaged in transit must be led
with the freight carrier as Kewaunee Scientic Corporation and its dealers are not
responsible for damages occurring during shipment.
Claims must be led with the freight carrier with fteen (15) days of delivery per the
United States Interstate Commerce Commission rules and regulations.
Limitations of Liability
All users of this equipment are required to become familiar with any regulations that
concern the disposal of waste materials in or surrounding water, land, or air, and to
comply with such regulations. The disposal and/or emission of substances used in
connection with this equipment may be governed by various federal, state, and/or
local entities. Kewaunee Scientic Corporation shall be held harmless with regard to
user’s compliance to regulations and/or use.
Page 61
Kewaunee Interceptor
®
Biological Safety Cabinet
Warranty Registration
PRODUCT DESCRIPTION KEWAUNEE DEALER
Model Number Company Name
I N T -
Serial Number Contact Name
Date of Purchase
/ /
CUSTOMER CONTACT INFORMATION
Company Name
Street Address
City State Zip Code
Contact Name
Phone Facsimile
(mm/dd/yyyy) Phone Number Extention
- -
- - - -
Email
OPTIONAL INFORMATION
How did you hear about us?
-
Do you currently own other Kewaunee Scientific Corporation products?
Would you like to receive Kewaunee Scientific Corporation product information?
Thank you for taking time to register your new product. Please contact Kewaunee Scientific
Corporation at 704-873-7202 or visit our website @ www.kewaunee.com if you have any questions.
Please mail or fax completed form to:
Kewaunee Scientific Corporation
Yes No
Yes No
2700 West Front Street
Statesville, North Carolina 28677
Tele: 704.873.7202 • Fax: 704.873.5160
Website: www.kewaunee.com
Email: kscmarketing@kewaunee.com
Page 62
Page 63
Page 64
LABORATORY PRODUCTS GROUP
P.O. Box 1842 • Statesville, NC 28687-1842
Phone: 704-873-7202 • Fax: 800-932-3296
Email: kscmarketing@kewaunee.com
© Copyright 2014 Printed in U.S.A. BSCUO-B2120-0714
Website: www.kewaunee.com
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