KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-mail: info@kern-sohn.com
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com
User instructions
Stereo microscope
KERN OSF-4
OSF 430, OSF 431, OSF 434, OSF 435
Version 1.0
01/2015
OSF-4-BA-e-1510
GB
KERN OSF-4
Version 1.0 01/2015
User instructions
Stereo microscope
Table of contents
1 Before use .................................................................................... 3
1.1 General notes ............................................................................................................................ 3
1.2 Notes on the electrical system ................................................................................................ 3
1.3 Storage ...................................................................................................................................... 4
1.4 Maintenance and cleaning ....................................................................................................... 5
2 Nomenclature ............................................................................... 6
3 Basic data ..................................................................................... 8
4 Assembly ...................................................................................... 9
5 Operation and functionality ....................................................... 11
5.1 Getting started ........................................................................................................................ 11
5.2 Adjusting the interpupillary distance ................................................................................... 11
5.3 Adjusting the magnification .................................................................................................. 11
5.4 Dioptre adjustment and focussing ....................................................................................... 12
5.5 Adjusting the stand ................................................................................................................ 13
5.6 Using eye cups / High Eye Point eyepieces ........................................................................ 14
5.7 Lighting control ...................................................................................................................... 15
5.8 Using external illumination units .......................................................................................... 15
5.9 Changing the bulb .................................................................................................................. 16
6 Optical data ................................................................................ 16
7 Features ...................................................................................... 16
8 Trouble shooting ........................................................................ 17
9 Service ........................................................................................ 18
10 Disposal ...................................................................................... 18
11 Further information .................................................................... 18
OSF-4-BA-e-1510 2
1 Before use
1.1 General notes
You must open the packaging carefully, to make sure that none of the accessories in
the packaging fall on the floor and get broken.
In general, microscopes should always be handled carefully because they are
sensitive precision instruments. When using or transporting the microscope it is
particularly important to avoid abrupt movements, as this may damage the optical
components.
You should also avoid getting dirt or finger prints on the lens surface, because in
most cases this will reduce image clarity.
To maintain the performance of the microscope, it must never be disassembled. So
components such as lenses and other optical elements should be left as they were
before use. Also the electrical parts in the base of the device must not be tampered
with, as in this area there is an additional risk of triggering an electric shock.
1.2 Notes on the electrical system
Before connecting to a mains power supply, you must make sure that you are using
the correct input voltage. The mains cable supplied has an external power supply.
The necessary values are shown on this power supply. If you do not comply with
these specifications, then fires or other damage to the device could occur.
The lighting switches must also be switched off before the mains cable is connected.
In this way you will avoid triggering an electric shock.
If you are using an extension cable, then the mains cable you use must be earthed.
When carrying out any procedures whereby you come into contact with the electrical
system of the device, such as, for example, changing the bulb, only carry out these
procedures when the power is disconnected.
3 OSF-4-BA-e-1510
1.3 Storage
You should ensure that the device is not exposed to direct sunlight, temperatures
which are too high or too low, vibrations, dust or a high level of humidity.
The ideal temperature range is between 0 and 40°C and a relative humidity of 85%
should not be exceeded.
The device should always be located on a rigid, smooth, horizontal surface.
For devices with pillar stands, the microscope holder must not be rotated back too
far. If you do this, there is a risk that the microscope could tip over.
When the microscope is not being used, you should fit the objective cap and cover
the microscope with the enclosed dust protective cover.
If the eyepieces are being stored separately, the protective caps must be fitted to the
tube connectors. In most cases, if dust and dirt gets inside the optical unit of a
microscope this can cause irreversible errors or damage.
The best way to store accessories which consist of optical elements, such as, for
example, eyepieces and objectives, is in a dry box with desiccant.
OSF-4-BA-e-1510 4
1.4 Maintenance and cleaning
In any event, the device must be kept clean and dusted regularly.
If any moisture should be occur, before you wipe down the device you must ensure
that the mains power is switched off.
When glass components become dirty, the best way to clean them is to wipe them
gently with a lint-free cloth.
To wipe oil stains or finger prints off the lens surface, moisten the lint free cloth with a
mixture of ether and alcohol (70 / 30 ratio) and use this to clean the lens.
You must be careful when handling ether and alcohol, as these are highly flammable
substances. You must therefore keep it away from naked flames and electrical
devices which can be switched on and off, and only use it in well-ventilated rooms.
However organic solutions of this type should not be used to clean other components
of the device. This could lead to damage to the paint finish. To do this, it is sufficient
to use a neutral cleaning product.
You could also use the following cleaning products to clean the optical components:
Special cleaner for optical lenses
Special optical cleaning cloths
Bellows
Brush
When handled correctly and checked regularly, the microscope should give many
years of efficient service.
Should repairs still be necessary, please contact your KERN dealer or our Technical
Department.
5 OSF-4-BA-e-1510
2 Nomenclature
Using the OSF 430 as an example
OSF-4-BA-e-1510 6
Special features of OSF 434, OSF 435
The focus dial has a coarse adjustment knob and a fine adjustment knob
7 OSF-4-BA-e-1510
3 Basic data
Optical system
Greenough
Dimmable lighting
No
Tube
angled at 45°
Interpupillary distance
55 – 75 mm
Dioptre adjustment
One-sided (left)
Packaging dimensions WxDxH
290x210x380 mm
Model
KERN
Tube
Eyepiece
Field of view
mm
Objective
Stand
Illumination
OSF 430
Binocular
WF 10x Ø 20 mm
Ø 20
1x / 3x
mechanical
1W LED (reflected light); 0,21W LED
(transmitted light)
OSF 431
Binocular
WF 10x Ø 20 mm
Ø 20
2x / 4x
mechanical
1W LED (reflected light); 0,21W LED
(transmitted light)
OSF 434
Binocular
WF 10x Ø 20 mm
Ø 20
1x / 2x / 3x
mechanical
1W LED (reflected light); 0,21W LED
(transmitted light)
OSF 435
Binocular
WF 10x Ø 20 mm
Ø 20
1x / 2x / 4x
mechanical
1W LED (reflected light); 0,21W LED
(transmitted light)
Standard configuration
OSF-4-BA-e-1510 8
4 Assembly
The first step is to position the microscope stand on a firm, level surface.
The holder is firmly attached to the pillar of the stand. You can then fit the
microscope head to the holder, by passing the objective through the holder ring until
the rest of the head is above the ring.
Please see section 5.5 for more details on adjusting the stand.
The head must then be fixed in a suitable position, using the small screw on the right
of the holder ring.
Ideally it is then parallel on the central axis of the stand base (see figure on page 10).
Both eyepieces are already fitted in the tube. You just need to remove the
protective film which covers them.
When doing this make sure that you do not touch the optical lenses with your
fingers.
In general you should avoid using two eyepieces with different magnifications.
When changing the eyepieces, you must also loosen the small silver screw under
the eyepiece on the tube connectors and once the new eyepiece is fitted, then it must
be re-tightened.
With regard to the use of transmitted light illumination you must make sure that
the frosted glass stand inlay is fitted in the centre of the stand base, so that the
transmitted light can be used correctly.
Additional optional attachments:
The eye cups supplied with the microscopes can be fitted to the eyepieces.
(see section 5.6)
9 OSF-4-BA-e-1510
Assembled stereo microscope
OSF-4-BA-e-1510 10
5 Operation and functionality
5.1 Getting started
After assembly, if the microscope is ready for use, then you must first establish a
power connection using the cable supplied.
Please see section 5.7 for more details on adjusting the lighting.
Do not forget to remove the cap from the bottom of the objective, so that you will then
be able to see a reflection of the object being observed in the eyepiece.
All important functions which relate to the use of the devices in this document are
described in the following sections.
5.2 Adjusting the interpupillary distance
Different users have different interpupillary distances. So each time a different
person uses the microscope, the gap between the two eyepieces must be readjusted.
While you are looking through the eyepieces, use one hand to hold the righthand or
lefthand prism housing firmly.
By rotating outwards or inwards, you can either increase or reduce the interpupillary
distance.
As soon as the lefthand and righthand visual fields exactly overlap each other, this is
the correct interpupillary distance.
5.3 Adjusting the magnification
As the KERN OSF-4 series covers stereo microscopes (without zoom), we will
describe how to set the magnification using the integrated and rotating objective.
For the devices in this series, you can select between two (OSF 430, OSF 431) or
three (OSF 434, OSF 435) different magnification factors at any time.
Chapter 6 “Optical data” gives information on the possible overall magnification which
the microscope can produce. It will also include the optional use of different
eyepieces.
11 OSF-4-BA-e-1510
5.4 Dioptre adjustment and focussing
A special feature of stereo microscopes is that they are fitted with an optical unit
which has a relatively high depth of field. In order to be able get the most benefit from
this feature, each user must synchronise the focussing mechanisms for themselves.
The steps to do this are described in the following section.
1. Place the object to be observed on the surface under the objective.
2. Use the objective to set the smallest possible magnification.
3. Look through the righthand eyepiece (without dioptre adjustment ring) with the
right eye and bring the object into focus by using the focus control dials.
4. Now set the largest magnification.
5. Once again, still only looking through the righthand eyepiece, bring the object into
focus
6. Then set the smallest possible zoom factor again.
7. Then look through the lefthand eyepiece with the left eye (with dioptre adjustment
ring) and bring the object into focus, by turning the dioptre adjustment ring left or
right at the right point.
8. In order to get the highest level of accuracy when adjusting the focus, you should
repeat steps 4-7.
9. In this way, the object being observed will be in focus on any magnification
setting.
OSF-4-BA-e-1510 12
5.5 Adjusting the stand
Torque of the focus wheels
(OSF 430, OSF 431)
You adjust the torque of the focus wheels by holding one of the two wheels in place
and using the other hand to turn the other wheel.
Depending on the direction of the turn, the torque will be increased or decreased.
On one hand, this function can help to make it easier to adjust the focus and on the
other hand it can prevent the microscope head from slipping down unintentionally. In
this way you can avoid possible damage which could occur if the objective lens and
the object being observed should collide.
Torque of the focus wheels
(OSF 434, OSF 435)
You can adjust the torque of the focus wheels by turning the ring which is fitted along
the axis of the lefthand focus wheel in a specific direction.
To do this you need a special wrench which is included with delivery.
The ring has holes which the wrench can hook into, so that you can then turn it in the
desired direction.
Depending on the direction of the turn, the torque will be increased or decreased.
On one hand, this function can help to make it easier to adjust the focus and on the
other hand it can prevent the microscope head from slipping down unintentionally. In
this way you can avoid possible damage which could occur if the objective lens and
the object being observed should collide.
13 OSF-4-BA-e-1510
5.6 Using eye cups / High Eye Point eyepieces
The eye cups supplied with the microscope can basically be used at all times, as
they screen out intrusive light, which is reflected from light sources from the
environment onto the eyepiece, and the result is better image quality.
But primarily, if eyepieces with a high eye point (particularly suitable for those who
wear glasses) are used, then it may also be useful for users who don’t wear glasses,
to fit the eye cups to the eyepieces.
These special eyepieces are also called High Eye Point eyepieces. They can be
identified by the glasses symbol on the side. They are also marked in the item
description by an additional “H” (example: HSWF 10x Ø 23 mm).
When fitting the eye cups, make sure that the dioptre setting is not moved. We would
therefore advise that you hold the dioptre adjustment ring on an eyepiece with one
hand while you fit the eye cup with the other.
Before using the microscope, users who wear glasses must remove the eye cups,
which you may find on High Eye Point eyepieces.
As the eye cups are made of rubber, you must be aware that when you are using
them, they can become slightly dirty through grease residues. In order to maintain
hygiene, we would therefore recommend that you clean the eye cups regularly (e.g.
with a damp cloth).
Eye cups
High Eye Point eyepiece
(identified by the glasses symbol)
OSF-4-BA-e-1510 14
5.7 Lighting control
All microscopes in the OSF-4 series have simple reflected and transmitted light
illumination.
With the mains cable connected you can switch the light units on and off using the
switches on the stand base as follows: for reflected light use the switch on the
right rear corner and for transmitted light use the switch on the left rear corner.
The light intensity cannot be adjusted.
5.8 Using external illumination units
If, when using a microscope in its standard version, the lighting is not suitable for the
application, then it often makes sense to fit an external lighting unit to overcome this
problem.
The lighting units which are suitable for devices of the OSF-4 series, are goose neck
lighting units (see figure). These are available as LED as well as halogen versions
and also have an on/off switch or different controller.
Typical goose neck lighting unit
Using goose neck lighting units
Depending on the requirements, a goose neck lighting unit is placed next to, in front
of or behind the microscope. The light source is in the housing of the unit and is
emitted through one or several optical fibre cables.
These cables are flexible and therefore offer a large number of positioning options,
so that the object being observed is perfectly illuminated.
15 OSF-4-BA-e-1510
5.9 Changing the bulb
Model outfit
Kern model
Order
number
OSF
430
OSF
431
OSF
434
OSF
435
Eyepieces
WF 5x / Ø 16.2 mm
○○
○○
○○
○○
OZB-A4101
WF 10x / Ø 20 mm
●●
●●
●●
●●
OZB-A4102
WF 15x / Ø 15 mm
○○
○○
○○
○○
OZB-A4103
WF 20x / Ø 10 mm
○○
○○
○○
○○
OZB-A4104
Stand
mechanical, with LED lighting (0.21W
transmitted light + 1W reflected light)
●
●
mechanical, incl. Fine adjustment knob,
with LED lighting
(0.21W transmitted light + 1W reflected
light)
●
●
Stand inlay
Frosted glass / Ø 74.5 mm
● ● ● ● OZB-A4813
black-white / Ø 74.5 mm
● ● ● ● OZB-A4814
External
illumination
For information on external lighting units, please see the Kern Optics main catalogue, from
page 68 and visit our website www.kern-sohn.com
LED
The devices in the OSF-4 series are all fitted with LED bulbs.
Due to the long service life of an LED lighting system, for these microscopes it will
not be necessary to simply change a bulb.
Problems with the lighting unit would therefore, in most cases, be caused by defects
in the electrical system. If this is the case, then our Technical Service will be able to
help.
6 Optical data
7 Features
● = Standard configuration ○ = Option
OSF-4-BA-e-1510 16
8 Trouble shooting
Problem
Possible causes
The lighting unit (if fitted) cannot be
switched on
The power cable is either not connected or
not connected correctly
The bulb is not fitted
The bulb has blown
The fuse has blown
The brightness control is set to the lowest
level
The bulb has blown
The wrong bulb has been used
The input voltage was too high
The bulb flickers
The bulb is not correctly fitted
The lamp is worn out
The bulb brightness is not sufficient
The wrong bulb has been used
The input voltage is too low
Problem
Possible causes
You can see two images
The magnifications of the eyepieces do not
match
There is dirt in the visual field
There is dirt on the object being observed
There is dirt on the eyepiece surface
The image is unclear
There is dirt on the objective surface
The focus wheels are jammed
The torque of the focus wheels is set too
high
The microscope head slips down
while you are viewing the object
The torque of the focus wheels is set too low
Eyes get tired easily
The dioptre adjustment is not correct
The brightness adjustment is not correct
Internationale Temperatur Korrektur Tabelle für °Brix (% Zuckergradient)
Das Ergebnis um die folgenden Werte korrigieren (Refraktometer muss korrekt kalibriert sein bei 20°C)
Reading °Brix
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0
10.0 -0.53 -0.56 - 0.59 -0.62 -0.65 -0.67 - 0.69 -0.71 -0.72 -0.73 - 0.74 -0.75 -0.75 -0.75 -0. 75 -0.75 -0.74 -0.73
11.0 -0.49 -0.52 - 0.54 -0.57 -0.59 -0.61 - 0.63 -0.64 -0.65 -0.66 - 0.67 -0.68 -0.68 -0.68 -0. 68 -0.67 -0.67 -0.66
12.0 -0.44 -0.47 - 0.49 -0.51 -0.53 -0.55 - 0.56 -0.57 -0.58 -0.59 - 0.60 -0.60 -0.61 -0.61 -0. 60 -0.60 -0.60 -0.59
13.0 -0.40 -0.41 - 0.43 -0.45 -0.47 -0.48 - 0.50 -0.51 -0.52 -0.52 - 0.53 -0.53 -0.53 -0.53 -0. 53 -0.53 -0.52 -0.52
14.0 -0.34 -0.36 - 0.38 -0.39 -0.40 -0.42 - 0.43 -0.44 -0.44 -0.45 - 0.45 -0.46 -0.46 -0.46 -0. 46 -0.45 -0.45 -0.44
15.0 -0.29 -0.31 - 0.32 -0.33 -0.34 -0.35 - 0.36 -0.37 -0.37 -0.38 - 0.38 -0.38 -0.38 -0.38 -0. 38 -0.38 -0.37 -0.37
16.0 -0.24 -0.25 - 0.26 -0.27 -0.28 -0.28 - 0.29 -0.30 -0.30 -0.30 - 0.31 -0.31 -0.31 -0.31 -0. 31 -0.30 -0.30 -0.30
17.0 -0.18 -0.19 - 0.20 -0.20 -0.21 -0.21 - 0.22 -0.22 -0.23 -0.23 - 0.23 -0.23 -0.23 -0.23 -0. 23 -0.23 -0.23 -0.22
18.0 -0.12 -0.13 - 0.13 -0.14 -0.14 -0.14 - 0.15 -0.15 -0.15 -0.15 - 0.15 -0.15 -0.15 -0.15 -0. 15 -0.15 -0.15 -0.15
19.0 -0.06 -0.06 - 0.07 -0.07 -0.07 -0.07 - 0.07 -0.08 -0.08 -0.08 - 0.08 -0.08 -0.08 -0.08 -0. 08 -0.08 -0.08 -0.07
20.0 0.00 0. 00 0.00 0. 00 0.00 0. 00 0.00 0. 00 0.00 0. 00 0.00 0. 00 0.00 0. 00 0.00 0.00 0.00 0.00
21.0 0.06 0. 07 0.07 0. 07 0.07 0. 07 0.08 0. 08 0.08 0. 08 0.08 0. 08 0.08 0. 08 0.08 0.08 0.08 0.07
22.0 0.13 0. 14 0.14 0. 14 0.15 0. 15 0.15 0. 15 0.16 0. 16 0.16 0. 16 0.16 0. 16 0.15 0.15 0.15 0.15
23.0 0.20 0. 21 0.21 0. 22 0.22 0. 23 0.23 0. 23 0.23 0. 24 0.24 0. 24 0.24 0. 23 0.23 0.23 0.23 0.22
24.0 0.27 0. 28 0.29 0. 29 0.30 0. 30 0.31 0. 31 0.31 0. 32 0.32 0. 32 0.32 0. 31 0.31 0.31 0.30 0.30
25.0 0.34 0. 35 0.36 0. 37 0.38 0. 38 0.39 0. 39 0.40 0. 40 0.40 0. 40 0.40 0. 39 0.39 0.39 0.38 0.37
26.0 0.42 0. 43 0.44 0. 45 0.46 0. 46 0.47 0. 47 0.48 0. 48 0.48 0. 48 0.48 0. 47 0.47 0.46 0.46 0.46
27.0 0.50 0. 51 0.52 0. 53 0.54 0. 55 0.55 0. 56 0.56 0. 56 0.56 0. 56 0.56 0. 55 0.55 0.54 0.53 0.52
28.0 0.58 0. 59 0.60 0. 61 0.62 0. 63 0.64 0. 64 0.64 0. 65 0.65 0. 64 0.64 0. 64 0.63 0.62 0.61 0.60
29.0 0.66 0. 67 0.68 0. 69 0.70 0. 71 0.72 0. 73 0.73 0. 73 0.73 0. 73 0.72 0. 72 0.71 0.70 0.69 0.68
30.0 0.74 0. 75 0.77 0. 78 0.79 0. 80 0.81 0. 81 0.81 0. 82 0.81 0. 81 0.81 0. 80 0.79 0.78 0.77 0.75
Temperatur °C
Electrical system
Optical unit
The interpupillary distance is not set
correctly
17 OSF-4-BA-e-1510
9 Service
All language versions contain a non-binding translation.
The original German document is the binding version.
If, after studying the user manual, you still have questions about commissioning or
using the microscope, or if unforeseen problems should arise, please get in touch
with your dealer. The device may only be opened by trained service engineers who
have been authorised by KERN.
10 Disposal
The packaging is made of environmentally-friendly materials, which you can dispose
of at your local recycling centre. Disposal of the storage box and device must be
carried out by the operator in accordance with all national or regional laws in force in
the location of use.
11 Further information
The illustrations may differ slightly from the product.
The descriptions and illustrations in this user manual are subject to change without
notice. Further developments on the device may lead to these changes.
OSF-4-BA-e-1510 18
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