OBE-1-BA-e-1510
KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-mail: info@kern-sohn.com
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com
User instructions
Compound laboratory
microscope
KERN OBE 111, OBE 112, OBE 113
Version 1.0
01/2015
OBE-1-BA-e-1510 2
GB
KERN OBE-1
Version 1.0 01/2015
User instructions
Compound microscopes
Table of contents
1 Before use .................................................................................... 3
1.1 General notes ............................................................................................................................ 3
1.2 Notes on the electrical system ................................................................................................ 3
1.3 Storage ...................................................................................................................................... 4
1.4 Maintenance and cleaning ....................................................................................................... 4
2 Nomenclature ............................................................................... 5
3 Technical data / model outfit ....................................................... 7
4 Assembly ...................................................................................... 8
4.1 Lenses ....................................................................................................................................... 8
4.2 Condenser ................................................................................................................................. 8
4.3 Head ........................................................................................................................................... 8
4.4 Eyepieces .................................................................................................................................. 8
5 Operation ...................................................................................... 9
5.1 Getting started .......................................................................................................................... 9
5.2 Adjust the gap between the eyes (only for OBE 112, OBE 113) .......................................... 9
5.3 Adjusting the magnification .................................................................................................... 9
5.4 Adjust the brightness............................................................................................................. 10
5.5 Dioptre compensation and focussing .................................................................................. 10
6 Storage ....................................................................................... 11
7 Maintenance ............................................................................... 11
7.1 Changing the bulb .................................................................................................................. 11
7.2 Changing the fuse .................................................................................................................. 11
8 Trouble shooting ........................................................................ 12
9 Service ........................................................................................ 14
10 Disposal ...................................................................................... 14
11 Further information .................................................................... 14
3 OBE-1-BA-e-1510
1 Before use
1.1 General notes
You must open the packaging carefully, to make sure that none of the accessories in
the packaging fall on the floor and get broken.
In general, microscopes should always be handled carefully because they are
sensitive precision instruments. When using or transporting the microscope it is
particularly important to avoid abrupt movements, as this may damage the optical
components.
You should also avoid getting dirt or finger prints on the lens surface, because in
most cases this will reduce image clarity.
To maintain the performance of the microscope, it must never be disassembled. So
components such as lenses and other optical elements should be left as they were
before use. Also the electrical parts in the base of the device must not be tampered
with, as in this area there is an additional risk of triggering an electric shock.
1.2 Notes on the electrical system
Before connecting to a mains power supply, you must make sure that you are using
the correct input voltage. The information to select the correct mains cable is located
on each device, on the rear of the product directly above the connection socket. You
must comply with this information. If you do not comply with these specifications, then
fires or other damage to the device could occur.
The main switch must also be switched off before the mains cable is connected. In
this way you will avoid triggering an electric shock.
If you are using an extension cable, then the mains cable you use must be earthed.
If the original fuse should blow, it must only be replaced by an appropriate fuse.
Suitable replacement fuses are included with the delivery.
When carrying out any procedures whereby you come into contact with the electrical
system of the device, such as, for example, changing the bulb or fuse, only carry out
these procedures when the power is disconnected.
OBE-1-BA-e-1510 4
1.3 Storage
You should ensure that the device is not exposed to direct sunlight, temperatures
which are too high or too low, vibrations, dust or a high level of humidity. The ideal
temperature range is between 0 and 40°C and a relative humidity of 85% should not
be exceeded. The device should always be located on a rigid, smooth, horizontal
surface.
When the microscope is not being used, you should fit the lens cap and cover the
microscope with the enclosed dust protective cover. If the eyepieces are being stored
separately, the protective caps must be fitted to the lens barrels. In most cases, if
dust and dirt gets inside the optical unit of a microscope this can cause irreversible
errors or damage. The best way to store accessories which consist of optical
elements, such as, for example, eyepieces and lenses, is in a dry box with desiccant.
1.4 Maintenance and cleaning
In any event, the device must be kept clean and dusted regularly. If any moisture
should be occur, before you wipe down the device you must ensure that the mains
power is switched off.
When glass components become dirty, the best way to clean them is to wipe them
gently with a lint-free cloth.
To wipe oil stains or finger prints off the lens surface, moisten the special lint free
cleaning cloth with a mixture of ether and alcohol (70 / 30 ratio) and use this to clean
the lens.
You must be careful when handling ether and alcohol, as these are highly flammable
substances. You must therefore keep it away from naked flames and electrical
devices which can be switched on and off, and only use it in well-ventilated rooms.
However organic solutions of this type should not be used to clean other components
of the device. This could lead to damage to the paint finish. To do this, it is sufficient
to use a neutral cleaning product.
You could also use the following cleaning products to clean the optical components:
Special cleaner for optical lenses
Special optical cleaning cloths
Bellows
Brush
When handled correctly and checked regularly, the microscope should give many
years of efficient service. Should repairs still be necessary, please contact your
KERN dealer or our Technical Department.
5 OBE-1-BA-e-1510
2 Nomenclature
Eyepiece
Head
Adjusting screw
Head
Lens revolving unit
Lens
Object holder
Specimen stage
Aperture adjustment
Condenser
Condenser
Field lens
Control dial
X – Y axis
Specimen
stage
Fine
adjustment
knob
Coarse
adjustment
knob
Main On/Off
switch
+ dimmer
Lens barrel connectors
OBE-1-BA-e-1510 6
Rear view
Mains
connection
Coarse
adjustment
knob
Fine
adjustment
knob
Fuse
Condenser
Focus dial
Adjusting ring
torque
Example OBE 111
(monocular)
7 OBE-1-BA-e-1510
3 Technical data / model outfit
OBE-1-BA-e-1510 8
4 Assembly
4.1 Lenses
The lenses are screwed into the lens revolving unit in such a way that when you turn
the revolving unit, the next lens for stronger magnification moves into position.
Please make sure that you do not touch the lenses with your fingers and that no dust
enters the apertures. For lenses which are marked “OIL”, you must use an immersion
oil with the lowest level of inherent fluorescence.
4.2 Condenser
The condenser is already permanently fitted in the device. The height can be
adjusted using the focus dial.
4.3 Head
The head is already fitted on the device. After loosening the adjusting screw, the
head can be rotated by 180 degrees, for example. Then the gently retighten the
adjusting screw. Please make sure that you do not touch the lenses with your fingers
and that no dust enters the apertures.
4.4 Eyepieces
For each eye, use an eyepiece with the same magnification. (Only applies to the
OBE 111 microscope, only one eyepiece available on this). Remove the plastic
protective caps and insert both eyepieces. Please make sure that you do not touch
the lenses with your fingers and that no dust enters the apertures.
9 OBE-1-BA-e-1510
5 Operation
5.1 Getting started
Plug the mains plug into the socket and plug the cold device plug into the device.
Switch on the main switch and use the dimmer (switch and dimmer are combined in
one rotary knob) to adjust the brightness of the bulb (at the beginning, select a low
level of brightnesss.
5.2 Adjust the gap between the eyes (only for OBE 112, OBE 113)
Different users have different gaps between the eyes. So each time a different
person uses the microscope, the gap between the two eyepieces must be readjusted.
While you are looking through the eyepieces, use one hand to hold the right or left
lens barrel firmly. By pulling them apart or pushing them together, you can either
increase or reduce the gap between the eyes. As soon as the left and right visual
fields fully overlap each other (only one circular image is visible), this is the correct
gap between the eyes.
5.3 Adjusting the magnification
Select the lens with the lowest magnification and make sure that the lens revolving
unit is properly clicked into place.
Place the test object on the specimen stage and fix it with the object holder, if
necessary.
Use the control dial for the X-Y axis specimen stage to position the sample in the
correct place (central under the middle of the lens).
Carefully raise the specimen stage using the coarse adjustment knob, until the
slide is almost touching the lens (observe from the side). The lens must not touch the
object holder (risk of glass breakage).
Look through the eyepieces (just one eyepiece with the OBE 111 device) and use the
coarse adjustment knob to lower it slowly until the object is visible. If necessary,
adjust the brightness by adjusting the bulb (potentiometer). Avoid overexposure.
Now use the fine adjustment knob to adjust the focus. For slides with low contrast
or a small number of objects, for example individual cells, we recommend that you
use a high-contrast standard slide to help you to make the adjustment to find the
correct level.
Once you have found a point which must be observed more closely, this must be
adjusted to be in the middle of the visual field (x/y adjustment of the stage). To be
able to view the required section of the object, this section must be positioned
centrally under the lens.
OBE-1-BA-e-1510 10
5.4 Adjust the brightness
Adjust condenser aperture
Adjust the height of the condenser until the image from the field diaphragm appears
clearly in the visual field. For some microscopes there is a risk that you will lift the
condenser up so high that it collides with the object holder. Care is needed when
doing this.
For each slide, use the aperture diaphragm of the condenser to find the very best
compromise between contrast and brightness for the microscopic image. The scale
on the condenser is to be regarded as a recommendation for each lens.
It is possible to alter the brightness of the bulb using the dimmer. The brightness is
always controlled by the bulb brightness.
It is possible to re-adjust the focus and x-y axis.
Observe the object.
5.5 Dioptre compensation and focussing
For models with dioptre setting:
Keep the eye on the fixed, non-adjustable lens barrel open and close the eye which
is on the adjustable lens barrel. Then adjust the focus for the open eye using the fine
adjustment knob.
Then open the other eye and shut the eye which had been open. Then adjust the
focus for the second eye on the dioptre setting. When doing this, do not touch the
fine adjustment knob.
If necessary, alter the brake action for the control knobs using the locking ring.
The rotary knobs on the lefthand side are connected to the rotary knobs on the
righthand side. Please only rotate on one side at a time, do not rotate right and left at
the same time in opposite directions, otherwise this will damage the drive.
Turning the lens revolving unit will bring the lens with the next magnification level
into position. Make sure that the lens clicks properly into place. When rotating the
lenses, check from the side to make sure that the glass holder does not get damaged
by the lens. Under normal conditions you should be able to select the next lens
immediately and the focus should be roughly at the same level. Make additional
adjustments with the fine adjustment knob. If when you rotate the lens it hits the
object holder, you must first lower the specimen stage and re-adjust it. For lenses
which work with oil immersion (these are marked with the word “oil”), first put the oil
drops on the slide, bring the lens into position and move it closer to the object holder.
The link between the object holder and lens is the oil. After use or before changing
the slide, remove the oil using a dust-free cloth.
11 OBE-1-BA-e-1510
6 Storage
It is important that the microscope is covered with the enclosed protective cover after
each use. In the long term, this is the only way to protect it from getting dirty. Ideally
the microscope will be used in an air-conditioned room. If the microscope is not used
for several days, then we would recommend that it is stored in a cupboard with its
protective cover. Dust and moisture are the greatest enemies of the microscope.
7 Maintenance
These microscopes are generally maintenance-free. Service work should be limited
to the replacement of bulbs, light sources and electrical fuses. Should a defect occur,
please contact your dealer or KERN Service.
7.1 Changing the bulb
The devices in the OBE range all have LED lighting. In general, LEDs are very longlasting. However, if you still suspect that a bulb is broken, then please contact our
Service Department.
7.2 Changing the fuse
Switch the device off and unplug it. Unscrew the fuse housing on the right next to the
mains cable socket, remove the old fuse and fit a new fuse. Screw the fuse housing
back in.
OBE-1-BA-e-1510 12
8 Trouble shooting
Problem
Possible causes
The bulb does not light
The mains plug is not correctly plugged
in
There is no power at the socket
Defective bulb / LED
Defective fuse
The bulb blows immediately
The specified
bulb or fuse has not been used.
The visual field is dark
The aperture diaphragm is not opened
wide enough
.
You cannot adjust the brightness
The brightness control has been set
incorrectly
The condenser is too low.
The visual field is dark or is not correctly
illuminated.
The lens has not been correctly
adjusted
The object revolving unit is not correctly
fitted.
The condenser is not correctly
fitted
A lens is being used which doesn’t
match the lighting area of the
condenser.
The bulb/LED is not fitted correctly.
The visual field of one eye does not
match that of the other eye.
The gap between the eyes is not
correctly
adjusted.
Different eyepieces are used for the right
and left side.
(Only OBE112 and OBE113)
The eyes are not used to
using a microscope.
Dioptre setting has not been carried out
correctly.
13 OBE-1-BA-e-1510
Blurred details
The aperture diaphragm is not opened
wide enough
.
Bad image
The condenser is too low.
Bad contrast
The lens does not belong to this
microscope.
Vignetted visual field
The front lens of the lens unit is
dirty.
An immersion object has been used
without immersion oil
The immersion oil contains air bubbles
The recommended immersion oil has not
been used.
Dirt / dust on the lens
Dirt / dust on the front lens
of the condenser.
Dirt or dust in the visual field
Dirt / dust on the eyepieces
Dirt / dust on the front lens
of the condenser.
Dirt / dust on the object.
One side of the image is blurred
The stage has not been assembled
correctly.
The lens is not positioned correctly on
the beam path.
The lens revolving unit is not correctly
fitted.
The upper side of the object
is facing down.
The image flickers
The lens revolving unit is not correctly
fitted
The lens is not positioned correctly on
the beam path.
The coarse adjustment knob is difficult to
turn
The torque adjusting ring is
overtightened.
The angle table is blocked by a
solid body.
The stage moves down on its own.
The torque adjusting ring is
not tight enough.
The fine adjustment knob moves on its
own
When you move the table, the image
becomes blurred
The stage was not correctly fitted
OBE-1-BA-e-1510 14
9 Service
If, after studying the user manual, you still have questions about commissioning or
using the microscope, or if unforeseen problems should arise, please get in touch
with your dealer. The device may only be opened by trained service engineers who
have been authorised by KERN.
10 Disposal
The packaging is made of environmentally-friendly materials, which you can dispose
of at your local recycling centre. Disposal of the storage box and device must be
carried out by the operator in accordance with all national or regional laws in force in
the location of use.
11 Further information
The illustrations may differ slightly from the product.
The descriptions and illustrations in this user manual are subject to change without
notice. Further developments on the device may lead to these changes.
All language versions contain a non-binding translation.
The original German document is the binding version.
Internationale Temperatur Korrektur Tabelle für °Brix (% Zuckergradient)
Das Ergebnis um die folgenden Werte korrigieren (Refraktometer muss korrekt kalibriert sein bei 20°C)
Reading °Brix
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0
10.0 -0.53 -0.56 -0.59 -0.62 -0.65 -0.67 -0.69 -0.71 -0.72 -0.73 -0.74 -0.75 -0. 75 -0.75 -0.75 -0.75 -0.74 -0.73
11.0 -0.49 -0.52 -0.54 -0.57 -0. 59 -0.61 -0.63 -0.64 -0.65 -0.66 -0.67 -0. 68 -0.68 -0.68 -0.68 -0.67 -0.67 -0. 66
12.0 -0.44 -0.47 -0.49 -0.51 -0. 53 -0.55 -0.56 -0.57 -0.58 -0.59 -0.60 -0. 60 -0.61 -0.61 -0.60 -0.60 -0.60 -0. 59
13.0 -0.40 -0.41 -0.43 -0.45 -0. 47 -0.48 -0.50 -0.51 -0.52 -0.52 -0.53 -0. 53 -0.53 -0.53 -0.53 -0.53 -0.52 -0. 52
14.0 -0.34 -0.36 -0.38 -0.39 -0. 40 -0.42 -0.43 -0.44 -0.44 -0.45 -0.45 -0. 46 -0.46 -0.46 -0.46 -0.45 -0.45 -0. 44
15.0 -0.29 -0.31 -0.32 -0.33 -0. 34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0. 38 -0.38 -0.38 -0.38 -0.38 -0.37 -0. 37
16.0 -0.24 -0.25 -0.26 -0.27 -0. 28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0. 31 -0.31 -0.31 -0.31 -0.30 -0.30 -0. 30
17.0 -0.18 -0.19 -0.20 -0.20 -0. 21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0. 23 -0.23 -0.23 -0.23 -0.23 -0.23 -0. 22
18.0 -0.12 -0.13 -0.13 -0.14 -0. 14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0. 15 -0.15 -0.15 -0.15 -0.15 -0.15 -0. 15
19.0 -0.06 -0.06 -0.07 -0.07 -0. 07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0. 08 -0.08 -0.08 -0.08 -0.08 -0.08 -0. 07
20.0 0.00 0. 00 0.00 0. 00 0.00 0. 00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0. 00 0.00 0. 00 0.00
21.0 0.06 0. 07 0.07 0. 07 0.07 0. 07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0. 08 0.08 0. 08 0.07
22.0 0.13 0. 14 0.14 0. 14 0.15 0. 15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0. 15 0.15 0. 15 0.15
23.0 0.20 0. 21 0.21 0. 22 0.22 0. 23 0.23 0.23 0.23 0.24 0.24 0.24 0.24 0.23 0. 23 0.23 0. 23 0.22
24.0 0.27 0. 28 0.29 0. 29 0.30 0. 30 0.31 0.31 0.31 0.32 0.32 0.32 0.32 0.31 0. 31 0.31 0. 30 0.30
25.0 0.34 0. 35 0.36 0. 37 0.38 0. 38 0.39 0.39 0.40 0.40 0.40 0.40 0.40 0.39 0. 39 0.39 0. 38 0.37
26.0 0.42 0. 43 0.44 0. 45 0.46 0. 46 0.47 0.47 0.48 0.48 0.48 0.48 0.48 0.47 0. 47 0.46 0. 46 0.46
27.0 0.50 0. 51 0.52 0. 53 0.54 0. 55 0.55 0.56 0.56 0.56 0.56 0.56 0.56 0.55 0. 55 0.54 0. 53 0.52
28.0 0.58 0. 59 0.60 0. 61 0.62 0. 63 0.64 0.64 0.64 0.65 0.65 0.64 0.64 0.64 0. 63 0.62 0. 61 0.60
29.0 0.66 0. 67 0.68 0. 69 0.70 0. 71 0.72 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0. 71 0.70 0. 69 0.68
30.0 0.74 0. 75 0.77 0. 78 0.79 0. 80 0.81 0.81 0.81 0.82 0.81 0.81 0.81 0.80 0. 79 0.78 0. 77 0.75
Temperatur °C
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