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OCM-1-BA-e-2211
KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-mail: info@kern-sohn.com
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com
User instructions
Biological inverted microscope
KERN OCM-1
OCM 161, OCM 165, OCM 166, OCM 167, OCM 168
Version 1.1
10/2022
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OCM-1-BA-e-2211 2
GB
KERN OCM-1
Version 1.1 10/2022
User instructions
Biological inverted microscope
Table of contents
1 Before use .................................................................................... 3
1.1 General notes ............................................................................................................................ 3
1.2 Notes on the electrical system ................................................................................................ 3
1.3 Storage ...................................................................................................................................... 4
1.4 Maintenance and cleaning ....................................................................................................... 5
2 Nomenclature ............................................................................... 6
3 Technical data / Features ............................................................ 8
4 Assembly .................................................................................... 10
4.1 Objectives ............................................................................................................................... 11
4.2 Eyepieces ................................................................................................................................ 11
4.3 Specimen stage ...................................................................................................................... 12
4.4 Condenser ............................................................................................................................... 13
5 Operation .................................................................................... 14
5.1 Getting started ........................................................................................................................ 14
5.2 (Pre-) focussing ...................................................................................................................... 15
5.3 Adjusting the interpupillary distance ................................................................................... 16
5.4 Dioptre adjustment ................................................................................................................. 16
5.5 Adjusting the magnification .................................................................................................. 17
5.6 Using eye cups ....................................................................................................................... 18
5.7 Adjusting the illumination ..................................................................................................... 19
6 Changing the bulb ..................................................................... 20
7 Changing the fuse ...................................................................... 21
8 Using optional accessories ....................................................... 22
8.1 Camera connection ................................................................................................................ 22
8.2 Phase-contrast units .............................................................................................................. 23
8.3 Fluorescence reflected light unit .......................................................................................... 25
8.3.1 Assembly and operation HBO versions: OCM 165 and OCM 166 .......................................... 34
8.3.2 Assembly and operation LED versions: OCM 167 and OCM 168 ........................................... 38
9 Trouble shooting ........................................................................ 40
10 Service ................................................................ ........................ 42
11 Disposal ...................................................................................... 42
12 Further information .................................................................... 42
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1 Before use
1.1 General notes
You must open the packaging carefully, to make sure that none of the accessories in
the packaging fall on the floor and get broken.
In general, microscopes should always be handled carefully because they are sensitive
precision instruments. When using or transporting the microscope it is particularly
important to avoid abrupt movements, as this may damage the optical components.
You should also avoid getting dirt or finger prints on the lens surface, because in most
cases this will reduce image clarity.
To maintain the performance of the microscope, it must never be disassembled. So
components such as lenses and other optical elements should be left as they were
before use. Also the electrical parts on the rear and base of the device must not be
tampered with, as in this area there is an additional risk of triggering an electric shock.
1.2 Notes on the electrical system
Before connecting to a mains power supply, you must make sure that you are using
the correct input voltage. The information to select the correct mains cable is located
on the device, on the rear of the product directly above the connection socket. You
must comply with this information. If you do not comply with these specifications, then
fires or other damage to the device could occur.
The main switch must also be switched off before the mains cable is connected. In this
way you will avoid triggering an electric shock.
If you are using an extension cable, then the mains cable you use must be earthed.
If the original fuse should blow, it must only be replaced by an appropriate fuse.
Suitable replacement fuses are included with the delivery.
When carrying out any procedures whereby you come into contact with the electrical
system of the device, such as, for example, changing the bulb or fuse, only carry out
these procedures when the power is disconnected.
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Under no circumstances should you touch the halogen bulb (for transmitted
illumination) or the HBO lamp (OCM 165, OCM 166) for the reflected light unit either
during operation or directly after use. These bulbs produce significant heat and
therefore there is a risk that the user could be severely burnt. So before handling the
bulbs, you must check that they have cooled down.
Furthermore the housing of the reflected light unit heats up during operation. That is
indicated by the warning symbol on the backside of the lamp housing. We recommend
to only carefully handle this housing during the usage and to let it cool down before it
is packed for transportation or covered by a protective hood.
1.3 Storage
You should ensure that the device is not exposed to direct sunlight, temperatures which
are too high or too low, vibrations, dust or a high level of humidity.
The ideal temperature range is between 0 and 40°C and a relative humidity of 85%
should not be exceeded.
The device should always be located on a rigid, smooth, horizontal surface.
When the microscope is not being used, you should cover it with the enclosed dust
protective cover. When doing this, the power supply is stopped by switching off at the
main switch and unplugging the mains cable. If the eyepieces are being stored
separately, the protective caps must be fitted to the tube connectors. In most cases, if
dust and dirt gets inside the optical unit of a microscope this can cause irreversible
errors or damage.
The best way to store accessories which consist of optical elements, such as, for
example, eyepieces and objectives, is in a dry box with desiccant.
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1.4 Maintenance and cleaning
In any event, the device must be kept clean and dusted regularly.
If any moisture should be occur, before you wipe down the device you must ensure
that the mains power is switched off.
When glass components become dirty, the best way to clean them is to wipe them
gently with a lint-free cloth.
To wipe oil stains or finger prints off the lens surface, moisten the lint free cloth with a
mixture of ether and alcohol (70 / 30 ratio) and use this to clean the lens.
You must be careful when handling ether and alcohol, as these are highly flammable
substances. You must therefore keep it away from naked flames and electrical devices
which can be switched on and off, and only use it in well-ventilated rooms.
However organic solutions of this type should not be used to clean other components
of the device. This could lead to damage to the paint finish. To do this, it is sufficient to
use a neutral cleaning product.
You could also use the following cleaning products to clean the optical components:
• Special cleaner for optical lenses
• Special optical cleaning cloths
• Bellows
• Brush
When handled correctly and checked regularly, the microscope should give many
years of efficient service.
Should repairs still be necessary, please contact your KERN dealer or our Technical
Department.
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2 Nomenclature
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3 Technical data / Features
OCM 161
Product dimension:
304×599×530 mm
Packing dimensions:
660x590x325 mm
Net weight:
13,5 kg
Gross weight:
18 kg
OCM 165 / 166 / 167 / 168
Product dimension:
304×782×530 mm
Packing dimensions:
1050x590x330 mm
Net weight:
21 kg
Gross weight:
28 kg
Input voltage:
AC 100-240V, 50-60Hz
Output voltage:
DC 1.2-6V
Fuse:
2A 5x20 mm
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4 Assembly
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4.1 Objectives
The nosepiece must be in its lowest position so that the objectives [1] can be screwed
into it. You can then pass the objectives through the opening of the specimen stage
and screw them into the nosepiece, so that when you turn the nosepiece in a clockwise
direction, the objective with the next strongest magnification appears. You must make
sure that you do not touch the lenses with your bare fingers and that no dust enters
the apertures.
To the screw locations which are not filled with an objective [2], you must attach the
protective cap.
4.2 Eyepieces
You must always use eyepieces with the
same magnification for both eyes. These are
simply placed onto the tube connectors, once
you have first removed the plastic protective
caps. There is a way of fixing them by an Allen
screw [1] for each eyepiece that is located on
the tube connector (see illustration). You
should always make sure that you do not
touch the lenses with your bare fingers and
that no dust enters the apertures.
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4.3 Specimen stage
The supplied stage plate [1] needs to be fitted to the opening of the specimen stage,
in order to get a support area for relatively small observation objects and to protect the
objectives which are located underneath.
Furthermore you can attach an object holder to one of the threads on stage surface
[2].
The scope of delivery standardly includes an attached mechanical stage [2].
It can be mounted on both sides of the specimen stage, so that the control wheels can
be operated, as the user wishes, on the left or on the right side. Also it provides the
possibility to place further object holders on the stage plate for using certain trays
(culture dishes).
The mounting is done by two screws [3], which then are fixating the attached stage
onto the left or right bottom side of the stage.
Furthermore you can apply additional object holders [4] in order to fix and move culture
dishes with special dimensions on the stage surface.
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4.4 Condenser
The condenser [2] must be mounted on the microscope housing underneath the lamp
housing. By using the Allen screw [1] it must be firmly attached onto the connection
point.
The condenser includes the following control elements:
- Color filter slide (see 5.7 Adjusting the illumination)
- Aperture diaphragm (see 5.7 Adjusting the illumination)
- Slot for phase contrast slide (see 8.2 Phase contrast unit)
For the connection of a microscope camera and the using of phase contrast or
fluorescence units, please see chapter 8 Using optional accessories.
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5 Operation
5.1 Getting started
The very first step is to establish a power connection using the mains plug. You should
first adjust the dimmer to a low level, so that when you look through the eyepiece for
the first time, your eyes are not immediately subject to a high level of light. You can
now switch on the lighting using the main switch.
The next step is to place an object holder with the sample on the specimen stage.
For the models of the OCM-1 series you can fixate the samples on the stage with help
of one of the available object holders (also see section 4.3 Specimen stage).
For the models of the OCM-1 series you can fixate the samples on the stage with help
of the object holder.
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5.2 (Pre-) focussing
When you are observing an object, you must have the correct distance to the objective
to achieve a sharp image.
In order to find this distance at the beginning (without other default settings of the
microscope) place the objective with the lowest magnification in the beam path, look
through the right eyepiece with the right eye and turn it slowly using the coarse
adjustment knob.
The simplest way of doing this would be to first raise the nosepiece (using the coarse
adjustment knob) to the top position and then lower it slowly. As soon as an image is
recognisable (no matter how sharp), then you should only adjust the focus using the
fine adjustment knob.
Adjusting the torque of the coarse and fine adjustment knob
Next to the left adjustment wheel for the coarse and fine adjustment knob there is a
ring (see illustration [1]) which you can use to alter the torque of these wheels. Turning
it in a clockwise direction reduces the torque and turning it in an anti-clockwise direction
increases it.
On one hand, this function can help to make it easier to adjust the focus and on the
other hand it can prevent the nosepiece from slipping down unintentionally.
Important:
In order to avoid damaging to the focussing system, the left and right adjustment
wheels for the coarse and fine adjustment knob must never be rotated at the same
time in opposite directions.
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5.3 Adjusting the interpupillary distance
With binocular viewing, the interpupillary distance must be
adjusted accurately for each user, in order to achieve a
clear image of the object.
While you are looking through the eyepieces, use your
hands to hold the righthand and lefthand tube housing
firmly. By pulling them apart or pushing them together,
you can either increase or reduce the interpupillary
distance (see illustration). As soon as the field of views of
the lefthand and righthand eyepieces completely overlap
each other, i.e., they combine to form a circular image, then the interpupillary distance
is set correctly.
5.4 Dioptre adjustment
The eye strengths of each eye of the microscope user can often be slightly different,
which in daily life has no consequences. But when using a microscope this can cause
problems in achieving precise focussing.
You can use a mechanism on both tube connectors (dioptre adjustment rings) to
compensate for this as follows.
1. Put the right dioptre adjustment ring to position 0.
2. Look through the right eyepiece with the right eye and bring the object into focus
by using the coarse and fine adjustment knob.
3. Then look through the left eyepiece with the left eye and use the lefthand dioptre
adjustment ring to focus the image.
To do this, you just need to turn the ring in both directions (see illustration), to find
out where the image is at its most focussed.
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5.5 Adjusting the magnification
After prefocussing has been carried out using the objective with the lowest
magnification (see section 5.2) you can then adjust the overall magnification using the
nosepiece, as necessary. By turning the nosepiece you can bring any one of the four
other objectives into the beam path.
When adjusting the nosepiece, you must take the following points into account:
- The required objective must be properly locked in place at all times.
- The nosepiece should not be rotated by holding individual objectives, you should
use the silver ring above the objectives.
- When rotating the nosepiece you must always make sure that the objective which
is about to be positioned in the beam path does not touch the object holder. This
can lead to significant damage to the objective lens.
We recommend that you always check from the side to make sure that there is
sufficient leeway. If this should not be the case, the nosepiece must be lowered
accordingly.
If you have focussed the object to be observed for a specific magnification, then if you
select the objective with the next greatest magnification, the object will be slightly out
of focus. Use the fine adjustment knob to make a slight adjustment and restore the
focus.
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5.6 Using eye cups
The eye cups supplied with the microscope can basically be used at all times, as they
screen out intrusive light, which is reflected from light sources from the environment
onto the eyepiece, and the result is better image quality.
But primarily, if eyepieces with a high eye point (particularly suitable for those who
wear glasses) are used, then it may also be useful for users who don’t wear glasses,
to fit the eye cups to the eyepieces.
These special eyepieces are also called High Eye Point eyepieces. They can be
identified by the glasses symbol on the side. They are also marked in the item
description by an additional “H” (example: HSWF 10x Ø 23 mm).
When fitting the eye cups, make sure that the dioptre setting is not moved. We would
therefore advise that you hold the dioptre compensation ring on an eyepiece with one
hand while you fit the eye cup with the other.
Before using the microscope, users who wear glasses must remove the eye cups,
which you may find on High Eye Point eyepieces.
As the eye cups are made of rubber, you must be aware that when you are using them,
they can become slightly dirty through grease residues. In order to maintain hygiene,
we would therefore recommend that you clean the eye cups regularly (e.g. with a damp
cloth).
Eye cups
High Eye Point eyepiece
(identified by the glasses symbol)
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5.7 Adjusting the illumination
To make sure that perfect image results are achieved during microscopic observation,
it is important that the direction of light of the microscope is optimised. The following
components of the transmitted illumination can be adjusted according to the application
requirements.
Aperture diaphragm
The aperture diaphragm is used to find the very best compromise between contrast
and resolution for the microscopic image.
To open and close this diaphragm you have to operate the lever [1] located at the
condenser.
Colour filter
The colour filter slide has one round aperture [2]. If needed, the supplied blue filter [1]
has to be inserted into this aperture. Subsequently the filter slide must be put into the
slot on the top of the condenser.
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6 Changing the bulb
You must not attempt to change the bulb immediately after the microscope has been
used, as the bulb will still be hot and so there is a risk that the user could be burnt.
Before changing the bulb the device must be switched off and unplugged.
To change the bulb the cover of the lamp housing [b] needs to be removed. Therefore
you previously have to loosen the appropriate fastening screw [a]. Now the defective
bulb can be pulled out of the socket and be replaced with a new one [c]. We
recommend that here you should also test again, to check that heat is no longer being
produced. After the cover has been reattached and fixated, the bulb replacement
procedure is complete.
Important:
When fitting the new bulb into the socket, it must only be handled with sterile gloves or
using the bulb packaging film. Grease and dust residue can have a negative effect on
the light quality and service life.
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7 Changing the fuse
The fuse housing is on the rear of the microscope below the mains power supply
socket. With the device switched off and unplugged, you can pull out the housing.
When doing this, it is helpful to use a screwdriver or similar tool. The defective fuse
can be removed from its housing and be replaced with a new one.
After that, you just need to insert the fuse housing back into the insertion point below
the mains power supply socket.
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8 Using optional accessories
8.1 Camera connection
Due to the trinocular tube it is possible to connect microscope cameras to the device,
in order to digitally record images or sequences of images of an object being observed.
After the plastic cover has been removed from the camera adapter connector on the
top of the microscope head, then a suitable adapter must be fitted.
In general there are two C-mount adapters available for this (1x and 0.5x magnification,
see Chapter 3 Features). After fitting one of these adapters it can be fixed with the
locking screw. A camera which has a C-mount thread is then screwed on top of the
adapter.
We recommend that you first adjust the field of view using the eyepieces on the device
for the existing requirements, and then carry out the observation using the microscope
camera (i.e. using the PC screen which is connected).
To do this, the trinocular wheel on the righthand side of the microscope head must be
set to the position “Camera”. The light from the microscope lighting is deflected so that
it is completely in the beam path for the camera, which causes a dark field of view in
the eyepieces. This means that it is not possible to simultaneously observe by the
eyepieces and PC screen.
For C-mount adapters, which have their own integrated magnification, the image which
is shown on the camera connected to the device can often have a different level of
focus compared with the image on the eyepiece.
In order to be able to bring both images into focus, the focus can be adjusted by those
adapters.
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8.2 Phase-contrast units
The standardly supplied phase contrast unit of the microscopes of the series OCM-1
consists of a PH objective (20x), a PH slide, a centring telescope and a green filter.
Optionally PH objectives with a magnification of 10x or 40x are available.
To use this, you need to place the PH objective into the beam path and push the PH
slide (the writing facing up) into the appropriate slot on the condenser.
The slide must be placed in a way that the one of the three snap in positions, which
suites the objective magnification, is located in the beam path.
A PH objective has a PH ring in its lens system, just like the individual positions of the
PH slides. Due to their size on one hand and their positioning on the other, the two
related rings must be aligned to one another in the beam path. The position of the ring
in the objectives cannot be altered, that of the rings in the condenser disc however can
be.
In case of a not optimum positioning of one of the ring pairs, the adjustment needs to
be carried out as follows.
a. First adjust the microscope using bright field mode.
b. Move the PH slide to the required position (e.g. “20x”).
c. Open the aperture diaphragm on the condenser to its maximum (slider to the left).
d. Bring the relevant PH objective (e.g. 20x) into the beam path.
e. Place the centring telescope onto one of the two tube connectors, in place of a
standard eyepiece.
f. Undo the fixing screw of the centring telescope and pull out (move) the front part of
the telescope, so that you can focus both phase rings in the field of view. Then
retighten the screws.
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In the field of view you will now see the image of a white (PH slide) and a black (PH
objective) ring. The black one is central and the white one possibly is pushed to
one side (see left illustration).
g. On the side of the PH slide, facing towards the user, there is always a thread directly
to the left and the right of the condenser housing. In each thread there is an
adjusting screw.
In both threads you now must insert an Allen screw driver (red), in order to engage
them. Then by turning the screws and at the same time watching the rings through
the centring telescope, move the white ring to the centre (see central illustration).
h. As soon as both the rings overlap each other (see right illustration) the adjustment
is successfully completed and can also be carried out for the pairs of rings of the
other magnifications.
i. After the adjustment, the centring telescope must be replaced by the standard
eyepiece, so that you are able to observe the object on the specimen stage in phase
contrast mode.
Depending on the preferences of the user, using a green filter can produce a more
effective and pleasant image. To achieve this, the green filter must be inserted into the
colour filter slide.
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8.3 Fluorescence reflected light unit
OCM 165 / 166 / 167 / 168
There are samples, which can be excited by light beams and thereby show emissions,
which have different wave lengths than the previous excitation beams. The wave
length of the emission is always bigger than the wave length of the excitation (Stokes
shift). This process is called fluorescence and can serve as the base of a microscopic
contrasting method. For the most common way to realise this, an upright light
microscope is extended by a fluorescence reflected light unit.
Principle
Depending on the sample there is an excitation light needed, that is contained in the
spectrum of the light source (HBO or LED). The excitation filter is only permeable for
the according wave length. After that the excitation light hits a dichroic mirror, which
reflects it towards the objective and the sample. After the sample absorbed the
excitation light, the emission of the fluorescent light occurs (with a bigger wave length
than the excitation light). The part of the fluorescent light, which is beamed into the
objective, can pass the dichroic mirror. The dichroic mirror additionally prevents the
remaining part of the excitation light from advancing towards the eyepieces. The barrier
filter finally eliminates all wave ranges out of the beam path not belonging to the
observed fluorescence. Thus the resulting image is just developed by the fluorescent
light emitted from the sample.
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Overview of wave lengths for excitation and emission per excitation filter
Blue line: Wave length excitation
Green line: Wave length emission
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Nomenclature
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Nomenclature (FL filter unit B / G: OCM 165, OCM 167)
Nomenclature (FL filter unit B /G / V / UV: OCM 166, OCM 168)
Fluorescence
filter slide
Beam path lock
Beam path lock
Fluorescence
filter slide
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Nomenclature (FL unit middle part: OCM 165 / 166 / 167 / 168)
Nomenclature (FL HBO lamp housing: OCM 165 / 166)
Field diaphragm
Connection point for
lamp housing
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Nomenclature (Power supply unit for HBO lamp: OCM 165 / 166)
Nomenclature (FL LED lamp housing: OCM 167)
Condenser
adjustment
LED lamp
slide
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Nomenclature (FL LED lamp housing: OCM 168)
Nomenclature (Power supply unit for LED lamp: OCM 167 / 168)
Condenser
adjustment
LED lamp
adjustment
wheel
Main
switch
Dimmer
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8.3.1 Assembly and operation HBO versions: OCM 165 and OCM 166
Assembly
In order to put the fluorescence reflected light unit into operation, the following steps
have to be carried out.
1. The main body of the FL unit is already firmly connected with the microscope
housing and towers above the rear side of the microscope.
2. On this point the lamp housing must be mounted by help of two Allen screws.
3. Install the connecting cable between lamp housing and power supply unit.
4. Establish the power supply by attaching the mains cable.
5. Put the UV protective cover on the front edge of the stage.
6. Insert the slide for the beam path lock into the appropriate slot.
7. Prior to the first use of the FL unit you need to mount the HBO lamp to the inside
of the of the lamp housing.
8. For this procedure please refer to “Changing the lamp” on page 36/37.
(Remove the mounted lamp dummy in advance)
Operation
Before you use the reflected light unit for special fluorescence applications, it will be
favourable to adjust the microscope in the bright field mode. This includes the placing
of the sample, adjusting the interpupillary distance, pre-focussing, dioptre adjustment,
etc. After that you can go on with the usage of the fluorescence reflected light unit.
1. When using a HBO lamp at first there must be ensured, that the beam path lock
has been activated (place the slide accordingly).
2. Establish the power supply.
3. Press the main switch. If existing, you have to press the ignition after that in order
to illuminate the lamp. It lasts about 15 minutes until the lamp has gained a
maximum and stable illuminating power.
4. After placing the sample, you can bring the needed objective into the beam path.
5. Set the required position of the FL filter slide.
OCM 165: Select between Blue and Green
OCM 166: Select between Blue, Green, Violet and Ultraviolet
6. Now the beam path lock can be deactivated (place the slide accordingly).
7. Start the observation.
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Control elements for illumination
The following control elements for the illumination play a role within the fluorescence
microscopy:
• Field diaphragm, condenser:
Optimisation of the contrast and the light yield
Important warnings for the usage of a HBO lamp
• During operation the lamp is showing a strong heat development. It should
be avoided to come into contact with the lamp housing while and after some
time of running the reflected light unit.
• The lamp must not in any circumstances be switched off during the preheating. It could cause a significant reduction of the lamps life time.
• Equally the lamp must not be switched on directly after a switch-off process.
• When pausing the observation you should always activate the beam path
lock in order to interrupt the light beam. The light spectrum of the HBO lamp
often can be harmful for microbes.
• Never look inside the eyepieces when the beam path is opened (by slide for
the beam path lock) and there is set no FL filter inside the beam path (both
levers pulled out) at the same time. There is the risk of blinding.
• A HBO lamp has a certain life time. The closer you push it to its limit, the
bigger the risk of an explosion of the lamp and setting free of toxic mercury
vapour. This must be avoided at all costs.
The following devices can provide information about the necessity of a lamp
replacement (This applies for 100W HBO lamps):
o Ampere meter on the power supply unit
As soon as the amperage exceeds or falls below a range of 4.0 A –
6.0 A → lamp replacement recommended
o Life time display on the power supply unit
As soon as 100 h are reached
→ lamp replacement highly recommended
o Before starting the operation with a new bulb, press the “Reset” button
on the power supply next to the Ampere meter with a thin pin shaped
tool.
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Centring the lamp (HBO)
When running the HBO lamp for a long time there is the possibility, that the holder of
the lamp is moving out of its centre due to the strong heat development. This
circumstance has to be corrected when it appears, because therefore the field of view
is no longer equally illuminated.
In this case you need to operate the centring screw for vertical positioning [1] as well
as the centring screw for horizontal positioning [2] accordingly, in order to set again the
optimum light direction.
Changing the lamp (HBO) (see figure on the next page)
1. Disconnect the FL reflected light unit from the power supply.
2. Check if the lamp housing has cooled down.
3. Loosen the screw for the cover of the lamp housing (only when it is cool).
4. Carefully remove the cover along with the lamp mounting from the rest of the
housing and put it, with the top facing down, onto a working surface.
5. Loosen the two fixing screws for the HBO lamp.
6. Replace the old lamp by a new one.
7. Tighten the fixing screws again.
8. Reattach the cover along with the lamp mounting and fix it with the screw.
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The new installed spare lamp may under no circumstances be touched
with naked hands. Impurities will benefit the risk of explosion during
operation.
If there should be nevertheless impurities, the lamp has to be cleaned. We
recommend for this purpose to use a lint-free cloth soaked with an ether alcohol
mixture (Proportion: 70/30).
Changing the fuse
The fuse housing is on the rear of the power supply unit below the mains power supply
socket. With the device switched off and unplugged, you can pull out the housing.
When doing this, it is helpful to use a screwdriver or similar tool. The defective fuse
can be removed from its housing and be replaced with a new one.
After that, you just need to insert the fuse housing back into the insertion point below
the mains power supply socket.
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OCM-1-BA-e-2211 38
8.3.2 Assembly and operation LED versions: OCM 167 and OCM 168
Assembly
In order to put the fluorescence reflected light unit into operation, the following steps
have to be carried out.
1. The main body of the FL unit is already firmly connected with the microscope
housing and towers above the rear side of the microscope.
2. On this point the lamp housing must be mounted by help of two Allen screws.
3. Install the connecting cable between lamp housing and power supply unit.
4. Establish the power supply by attaching the mains cable.
5. Put the UV protective cover on the front edge of the stage.
6. Insert the slide for the beam path lock into the appropriate slot.
7. The LED lamps in the lamp housing are already installed.
Operation
Before you use the reflected light unit for special fluorescence applications, it will be
favourable to adjust the microscope in the bright field mode. This includes the placing
of the sample, adjusting the interpupillary distance, pre-focussing, dioptre adjustment,
etc. After that you can go on with the usage of the fluorescence reflected light unit.
1. When using the UV LED lamp, at first there must be ensured, that the beam path
lock has been activated (place the slide accordingly).
2. Establish the power supply.
3. Press the main switch.
4. After placing the sample, you can bring the needed objective into the beam path.
5. Set the required positions of the FL filter slide and the LED lamp slide (OCM 167) /
wheel (OCM 168).
OCM 167: Select between Blue and Green
OCM 168: Select between Blue, Green, Violet and Ultraviolet
The color of the selected filter must always be matching the color of the selected
LED lamp.
6. Now the beam path lock can be deactivated (place the slide accordingly).
7. Start the observation.
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39 OCM-1-BA-e-2211
Control elements for illumination
The following control elements for the illumination play a role within the fluorescence
microscopy:
• Field diaphragm, condenser:
Optimisation of the contrast and the light yield
Changing the fuse (if existing)
The fuse housing is on the rear of the power supply unit below the mains power supply
socket. With the device switched off and unplugged, you can pull out the housing.
When doing this, it is helpful to use a screwdriver or similar tool. The defective fuse
can be removed from its housing and be replaced with a new one.
After that, you just need to insert the fuse housing back into the insertion point below
the mains power supply socket.
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OCM-1-BA-e-2211 40
9 Trouble shooting
Problem
Possible causes
The bulb does not light
The mains plug is not correctly plugged in
There is no power at the socket
Defective bulb
Defective fuse
The bulb blows immediately
The specified bulb or fuse has not been used
The field of view is dark
The aperture diaphragm and/or field
diaphragm are not opened wide enough
The selector switch for the beam path is set
to “Camera”
The condenser is not correctly centred
You cannot adjust the brightness
The brightness control has been set
incorrectly
The condenser has not been correctly
centred
The field of view is dark or is not
correctly
illuminated
The objective is not positioned correctly on
the beam path
The selector switch for the beam path is
between two settings
The nosepiece is not correctly fitted
An objective is being used which doesn’t
match the lighting area of the condenser
The condenser has not been correctly
centred
The field diaphragm is closed too tightly
The bulb is not correctly fitted
The field of view of one eye does not
match that of the other eye
The interpupillary distance is not correctly
adjusted
Dioptre setting has not been carried out
correctly
Different eyepieces are used for the
righthand and lefthand side
The eyes are not used to using a microscope
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41 OCM-1-BA-e-2211
Problem
Possible causes
Blurred details
Bad image
Bad contrast
Vignetted field of view
The aperture diaphragm is not opened wide
enough
The objective does not belong to this
microscope
The front lens of the objective is dirty
The condenser is not correctly centred
Dirt / dust on the objective
Dirt /dust on the front lens of the condenser
Dirt or dust in the field of view
Dirt / dust on the eyepieces
Dirt / dust on the front lens of the condenser
Dirt / dust on the object
One side of the image is blurred
The stage was not correctly fitted
The objective is not positioned correctly on
the beam path
The nosepiece is not correctly fitted
The image flickers
The nosepiece is not correctly fitted
The objective is not positioned correctly on
the beam path
The condenser has not been correctly
centred
The coarse adjustment knob is difficult to
turn
The rotational resistance brake is too
tight
The angle table is blocked by a
solid body
The stage moves down on its own
The fine adjustment knob moves on its
own
The rotational resistance brake is not tight
enough
When you move the table, the image
becomes blurred
The stage was not correctly fitted
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10 Service
If, after studying the user manual, you still have questions about commissioning or
using the microscope, or if unforeseen problems should arise, please get in touch with
your dealer. The device may only be opened by trained service engineers who have
been authorised by KERN.
11 Disposal
The packaging is made of environmentally-friendly materials, which you can dispose
of at your local recycling centre. Disposal of the storage box and device must be carried
out by the operator in accordance with all national or regional laws in force in the
location of use.
12 Further information
The illustrations may differ slightly from the product.
The descriptions and illustrations in this user manual are subject to change without
notice. Further developments on the device may lead to these changes.
All language versions contain a non-binding translation.
The original German document is the binding version.
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