Jasco V-530, V-560, V-550, V-570 Instruction Manual

P/N:0302-0304C January 2003

V-530/550/560/570

Spectrophotometer

Instruction Manual

V -500 for W indow s

Safety Considerations

To ensure operation safety, this instrument must be operated correctly and maintained according to a regular schedule. Carefully read to fully understand all safety precautions in this manual before operating the instrument. Please take a moment to understand what the signal words WARNING!, CAUTION, and Note mean in this manual.

(1) Safety symbols

Instruction manual symbol. If the product is marked with this symbol, refer to the instrument manuals to protect the instrument against damage.

WARNING A WARNING indicates an potentially hazardous situation which, if not avoided,

could result in death or serious injury

CAUTION A CAUTION indicates a potentially hazardous situation which, if not avoided,

may result in minor or moderate injury. It may also be used to alert against damaging the equipment.

Do not proceed beyond a WARNING or CAUTION notice until you understand the hazardous conditions and have taken the appropriate steps.

Note A Note provides additional information to aid the operator in obtaining optimal

instrument performance.

(2) Warning Label

Warning labels are attached at several locations on this instrument. Do not remove,

deface or damage the warning labels. If a warning label peels off the instrument or becomes illegible, contact your local JASCO distributor and state the part number of the label you want to replace.

1) Warning for FUSE

(Fig.1 or Fig. 2)

Part No.: 0822-0120A

2) Warning for GROUND

(Fig.1 or Fig.2)

Part No.: 0822-0125A

Warning for FUSE Warning for GROUND

Figure 1 Warning Labels on the V-530 Back Panel

WARNING

FOR CONTINUED PROTECTION AGAINST RISK OF FIRE, REPLACE ONLY WITH FUSE OF THE SPECIFIED TYPE AND CURRENT RATINGS.

Only use fuses of the specified rating to protect both operator and instrument from fire and other hazards. When replacing a fuse, refer to the hardware manual (4.4.1 Replacing the fuse). The warning labels that pertain to fuse ratings are located on the back panel of the instrument.

WARNING

MAINS PLUG GROUND PIN OR GROUND TERMINAL MUST BE CONNECTED TO GROUND.

This instrument must be grounded correctly; either the mains plug ground pin or through the ground terminal on the rear panel of the instrument.

iii

Warning for FUSEWarning for GROUND

Figure 2 Warning Labels on the V-550/560/570 Back Panel

(2) Warning for carrying

The weight (in kilograms) of each instrument is as follows:

V-530 V-550 V-560 V-570

16.0 30.5 32.0 33.0

When moving the instrument, hold the handgrips at the bottom of the instrument

firmly (see Fig. 3).

Handgrips

Figure 3 Example: V-550/560/570 side view

Regulatory Statements

CE Notice

Marking by the symbol

indicates compliance of this JASCO system to the EMC (Electromagnetic Compatibility) and Low Voltage Directives of the European Community. This symbol indicates that this JASCO system meets the relevant basic safety and health requirements of the EC Directive based on the following technical standards:

· EN55011 ---- ”Limits and Methods of Measurement of Radio Interference Characteristics of

Information Technology Equipment.” ---- Group 1, Class A.

WARNING: This is a Class A product. In a domestic environment this product may cause

radio interference, in which case the user may be required to take adequate measures.

· EN50082-1 -- "Electromagnetic compatibility -- Generic immunity standard Part 1:

Residential, commercial, and light industry."

· IEC61000-4-2 -- "Electromagnetic compatibility for industrial-process measurement and

control equipment Part 2: Electrostatic discharge requirements."-- Severity level 3.

· IEC61000-4-3 -- "Electromagnetic compatibility for industrial-process measurement and

control equipment Part 3: Radiated electromagnetic field requirements." -- Severity level 2.

· IEC61000-4-4 -- "Electromagnetic compatibility for industrial-process measurement and

control equipment Part 4: Electrical fast transient/burst requirements."-- Severity level 3.

· IEC1010-1: 1990 + Amd.1: 1992 + Amd.2: 1995 -- Safety requirements for electrical

equipment for measurements, control and laboratory use.

· IEC61000-3-2: 1995 + Amd.1: 1998 + Amd.2: 1998 --- "Electromagnetic compatibility:

Limits for harmonic current emissions (equipment input current up to and including 16A per phase).

· A "Declaration of Conformity" in accordance with the above standards has been made and

is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060 CREMELLA (LC), Italy.

FCC Statement (for USA only)

Federal Communications Commission Radio Frequency Interference Statement

W A R N IN G : T his equipm ent generates, uses, and can radiate radio frequency energy. If it is not installed

and used in accordance w ith the instruction m an ual, it m ay cause interference to radio com m unications. It has been tested and found to com ply w ith the lim its for a C lass A com puting device pursuant to P art 15 of F C C R ules, w hich are intended to provide reasonable protection against such interference w hen operated in a com m ercial environm ent. O peration of this equipm ent in a residential area is likely to cause interference, in w hich case the u ser at his ow n expense w ill be required to take w ha tever m easures m ay be required to correct the interference.

Preface

This instruction manual is your guide for using this instrument. It instructs first-time users on how to use the instrument, and serves as a reference for experienced users.

Before using the instrument, please read this instruction manual carefully, and make sure that the contents are fully understood. This manual should be easily accessible to the operator at all times during instrument operation. When not using the instrument, keep this manual in a safe place. If this instruction manual becomes lost, order a replacement from your local JASCO distributor.

The instruction manual is divided into following volumes:

1) Hardware manual: Describes the principles of operation, specifications, maintenance, troubleshooting of this instrument.

2) Operation manual: Describes the configuration of this measument program, functions, and operating procedures.

3) Analysis: Describes the configuration of spectra analysis and file viewer program. (DS type)

N ote: The operating procedure varies w ith the type of instrum en t. O peration is describ ed by type. R ead the

relevant portion.

Installation Requirements

To ensure operation safety, observe the following conditions:

(1) Do not operate the instrument under voltage fluctuations exceeding 10% of the

recommended line voltage. Otherwise, the instrument may not function properly.

(2) Frequency or spike noise in the power supply should be minimal.

(3) Ensure that the instrument is grounded.

(4) Operate the instrument in a temperature range of 10 ~ 35°C.

(5) Operate the instrument in a humidity range of 35 ~ 85%(RH). If ambient humidity

exceeds 85%(RH), condensation may deteriorate optical components.

(6) Operate the instrument in an atmospheric pressure range of 950 ~ 1060hPa.

(7) Avoid strong magnetic fields and sources of high frequency. The instrument may not

function properly when near a strong magnetic field or high frequency source.

(8) Avoid vibration from vacuum pumps, electric motors, processing equipment and

machine tools.

(9) Avoid dust and corrosive gas. Do not install the instrument where it may be exposed to

dust, especially in locations exposed to outside air or ventilation outlets that discharge dust particles.

(10) Do not install the instrument in a location where it may be exposed to direct sunlight.

(11) Install the instrument in a horizontal and stable position. (This includes a table or desk

upon which the instrument is installed.)

(12) Ensure that no air conditioner blows air directly onto the instrument. This may prevent

stable measurement.

(13) Install the instrument in a location that allows easy access for maintenance.

Note: The above conditions do not guarantee optimal performance of this instrument.

vii

Servicing

Contact your local JASCO distributor for instrument servicing. In addition, contact your JASCO distributor before moving the instrument to another location. Consumable parts should be ordered according to part number from your local JASCO distributor. If a part number is unknown, give your JASCO distributor the model name and serial number of your instrument.

Do not return contaminated products or parts that may constitute a health hazard to JASCO employees.

Notices

(1) JASCO shall not be held liable, either directly or indirectly, for any consequential damage

incurred as a result of product use.

(2) Prohibitions on the use of JASCO software

· Copying software for purposes other than backup

· Transfer or licensing of the right to use software to a third party

· Disclosure of confidential information regarding software

· Modification of software

· Use of software on multiple workstations, network terminals, or by other methods

(not applicable under a network licensing agreement concluded with JASCO)

(3) The contents of this manual are subject to change without notice for product improvement.

(4) This manual is considered complete and accurate at publication.

(5) This manual does not guarantee the validity of any patent rights or other rights.

(6) In general, company names and product names are trademarks or registered trademarks

of the respective companies.

(7) JASCO and the JASCO logo are registered trademarks of JASCO Corporation

viii

Limited Warranty

Products sold by JASCO, unless otherwise specified, are warranted for a period of one year from the date of shipment to be free of defects in materials and workmanship. If any defects in the product are found during this warranty period, JASCO will repair or replace the defective part(s) or product free of charge.

THIS WARRANTY DOES NOT APPLY TO DEFECTS RESULTING FROM THE FOLLOWING:

1) IMPROPER OR INADEQUATE INSTALLATION

2) IMPROPER OR INADEQUATE OPERATION, MAINTENANCE, ADJUSTMENT OR CALIBRATION

3) UNAUTHORIZED MODIFICATION OR MISUSE

4) USE OF CONSUMABLE PARTS NOT SUPPLIED BY AN AUTHORIZED JASCO DISTRIBUTOR

5) CORROSION DUE TO THE USE OF IMPROPER SOLVENTS, SAMPLES, OR DUE TO SURROUNDING GASES

6) ACCIDENTS BEYOND JASCO’S CONTROL, INCLUDING NATURAL DISASTERS

This warranty does not cover the consumable parts listed below:

1) Deuterium lamp, tungsten lamp, xenon lamp and other light sources

2) Mirrors in the light source section, and cell windows

3) Fuses, batteries, glassware, chart paper and ink

The LC system only contains:

4) Plunger seals, needle seals, cell window gaskets, valve seals, disk seals and other seal

materials

5) Tubing and fittings (e.g., ferrules, compression screws), and filters (e.g., inlet filters, line

filters, other solvent filters)

6) Pre-columns and guard columns

THE WARRANTY FOR ALL PARTS SUPPLIED AND REPAIRS PROVIDED UNDER THIS WARRANTY EXPIRES ON THE WARRANTY EXPIRATION DATE OF THE ORIGINAL PRODUCT. FOR INQUIRIES CONCERNING REPAIR SERVICE, CONTACT YOUR JASCO DISTRIBUTOR AFTER CONFIRMING THE MODEL NAME AND SERIAL NUMBER OF YOUR INSTRUMENT.

JASCO Corporation 2967-5, Ishikawa-machi, Hachioji-shi Tokyo 192-8537 JAPAN

Contents

Safety Considerations.................................................................................................................i

Regulatory Statements .............................................................................................................. iv

Preface ....................................................................................................................................... v

Installation Requirements........................................................................................................ vi

Servicing ..................................................................................................................................vii

Notices...................................................................................................................................... vii

Limited Warranty.................................................................................................................... viii

1. Introduction ........................................................................................................................... 1

1.1 Layout of this Manual....................................................................................................... 1

1.2 Foreword and Notation Used............................................................................................ 1

1.3 Overview of [Spectra Manager] ....................................................................................... 2

2. Starting and exiting programs and [Spectra Manager] ....................................................... 4

2.1 Startup............................................................................................................................... 4

2.1.1 Turning ON the spectrophotometer........................................................................................ 4

2.1.2 PC and WindowsÒ startup ..................................................................................................... 4

2.1.3 [Spectra Manager] startup ...................................................................................................... 4

2.2 Exiting .............................................................................................................................. 6

2.2.1 Exiting measurement or spectra analysis program ................................................................. 6

2.2.2 PC and spectrophotometer shutdown ..................................................................................... 7

2.3 [Spectra Manager] Menu .................................................................................................. 8

3. Quantitative Analysis and Spectrum Measurement........................................................... 10

3.1 Quantitative Analysis Introduction................................................................................. 10

3.1.1 Quantitative analysis program overview .............................................................................. 10

3.1.1.1 Quantitative analysis program....................................................................................... 10

3.1.1.2 Quantitative analysis operation ..................................................................................... 11

3.1.2 Program startup .................................................................................................................... 11

3.1.3 Calibration curve creation .................................................................................................... 12

3.1.4 Calibration curve modification............................................................................................. 16

3.1.5 Saving quantitative analysis method .................................................................................... 17

3.1.6 Unknown sample measurement............................................................................................ 18

3.1.7 Saving data sheet .................................................................................................................. 19

3.1.8 Printing results...................................................................................................................... 19

3.1.9 Exiting quantitative analysis ................................................................................................ 20

3.2 Spectrum Measurement .................................................................................................. 21

3.2.1 Procedural overview............................................................................................................. 21

3.2.2 [Spectrum measurement] program startup............................................................................ 21

3.2.3 Setting measurement parameters.......................................................................................... 22

3.2.4 Setting the baseline(or Measurement) .................................................................................. 23

3.2.5 Sample measurement............................................................................................................ 25

3.2.6 Spectrum save....................................................................................................................... 26

3.2.7 Printing results...................................................................................................................... 26

3.2.8 Instrument shutdown ............................................................................................................ 27

4. [Quantitative Analysis]........................................................................................................ 28

4.1 [File] menu ..................................................................................................................... 30

4.1.1 [New...]................................................................................................................................. 30

4.1.2 [Open...]................................................................................................................................ 32

4.1.3 [Save].................................................................................................................................... 32

4.1.4 [Save As...] ........................................................................................................................... 33

4.1.5 [Page Setup...] ...................................................................................................................... 34

4.1.6 [Print Setup...] ...................................................................................................................... 34

4.1.7 [Print...] ................................................................................................................................ 35

4.1.8 [Exit]..................................................................................................................................... 35

4.2 [Method] menu ............................................................................................................... 36

4.2.1 [New...]................................................................................................................................. 36

4.2.2 [Open...]................................................................................................................................ 40

4.2.3 [Save As...] ........................................................................................................................... 41

4.2.4 [Modify...]............................................................................................................................. 41

4.2.5 [Information...] ..................................................................................................................... 42

4.3 [Measurement] menu...................................................................................................... 42

4.3.1 [Measurement...]................................................................................................................... 42

4.3.2 [Parameters...]....................................................................................................................... 43

4.3.3 [Blank Correction...]............................................................................................................. 43

4.4 [Edit] menu ..................................................................................................................... 44

4.4.1 [Copy Picture] ...................................................................................................................... 44

4.4.2 [Copy Bitmap]...................................................................................................................... 44

4.4.3 [Copy Text]........................................................................................................................... 44

4.4.4 [Invalid]................................................................................................................................ 44

4.4.5 [Data Input...] ....................................................................................................................... 44

4.4.6 [Title...]................................................................................................................................. 44

4.5 [View] menu ................................................................................................................... 44

4.5.1 [Font...]................................................................................................................................. 45

4.5.2 [Format...]............................................................................................................................. 46

4.5.3 [Cell Width...]....................................................................................................................... 46

4.5.4 [Scale...]................................................................................................................................ 46

4.5.5 [Pattern...] ............................................................................................................................. 47

4.5.6 [Grid...]................................................................................................................................. 47

4.5.7 [Style...] ................................................................................................................................ 48

4.5.8 [Marker...]............................................................................................................................. 49

4.6 [Window] menu ..............................................................................................................49

4.7 [Help] menu.................................................................................................................... 49

5. [Spectrum Measurement].................................................................................................... 50

5.1 [Measurement] menu...................................................................................................... 51

5.1.1 [Start].................................................................................................................................... 51

5.1.2 [Parameter...] ........................................................................................................................ 51

5.1.2.1 [Parameters] dialog box................................................................................................. 51

5.1.2.2 [Data File] dialog box.................................................................................................... 55

5.1.3 [Baseline...]........................................................................................................................... 56

5.1.4 [Move Wavelength...] ........................................................................................................... 58

5.1.5 [Auto Zero]........................................................................................................................... 59

5.1.6 [Exchange Wavelength...]..................................................................................................... 59

5.1.7 [Wavelength for Grating Exchange...].................................................................................. 59

5.1.8 [Exit]..................................................................................................................................... 59

5.2 [Help] menu.................................................................................................................... 59

6. [Time Course Measurement] ..............................................................................................60

6.1 [Measurement] menu...................................................................................................... 61

6.1.1 [Start].................................................................................................................................... 61

6.1.2 [Parameter...] ........................................................................................................................ 61

6.1.2.1 [Parameter] dialog box .................................................................................................. 61

6.1.2.2 [Data File] dialog box.................................................................................................... 63

6.1.3 [Move Wavelength...] ........................................................................................................... 63

6.1.4 [Auto Zero]........................................................................................................................... 63

6.1.5 [Exit]..................................................................................................................................... 63

6.2 [Help] menu.................................................................................................................... 64

7. [Fixed Wavelength Measurement]...................................................................................... 65

7.1 [Measurement] menu...................................................................................................... 66

7.1.1 [Start].................................................................................................................................... 66

7.1.2 [Blank] ( ) [Blank Correct] ([Correct]) ...................................................................... 66

7.1.3 [Parameter...] ........................................................................................................................ 67

7.1.4 [Goto Wavelength...] ............................................................................................................ 69

7.1.5 [Auto Zero]........................................................................................................................... 69

7.1.6 [Exit]..................................................................................................................................... 69

7.2 [Data] .............................................................................................................................. 69

7.3 [Help] menu.................................................................................................................... 69

8. [Abs/%T Meter] ...................................................................................................................70

9. [Environment] ..................................................................................................................... 72

9.1 [Hardware Setting] ......................................................................................................... 72

9.2 [Diagnosis] menu............................................................................................................ 73

9.3 [Accessories Setting] ...................................................................................................... 74

9.4 [Wavelength Correction] ................................................................................................ 75

10. Appendix ............................................................................................................................ 76

10.1 Spectra Manager Installation ........................................................................................ 76

10.1.1 Before installation .............................................................................................................. 76

10.1.2 Installing Spectra Manager from Floppy Disk ................................................................... 76

10.1.2.1 Starting up SETUP.EXE.............................................................................................. 76

10.1.2.2 Inputting operator name or company name................................................................. 79

10.1.2.3 Setting the program directory...................................................................................... 80

10.1.2.4 Setting the data directory............................................................................................. 80

10.1.2.5 Copying files to hard disk ........................................................................................... 81

10.1.3 Installing Spectra Manager from CD-ROM................................................................... 82

10.2 Setting the Serial Port (RS-232C) ................................................................................ 84

xii

1. Introduction

1.1 Layout o f this M anual

T his section describes the layout and fun ction of this m anual. T he V -500 for W indow sÒ in struction m anual consists of 10 sections, including this on e. R ead this m anual carefully in o rder to ensure a full understanding of th e operating procedures before using the V -530/55 0/560/5 70.

F or [S pectra A nalysis] program , refer to the "S pectra A nalysis/F ile V iew er Instructio n M anual". F or [JA S C O C anvas] program , refer to the "JA S C O C anvas Instruction M anu al".

H ereafter, this m anual w ill refer to the V -500 for W indow s as the V -500W , and to M icrosoft W indow s as W indow s.

S ection 1. Introduction

T his section expla ins th e w riting syntax an d d isplay configuration used in this m anual. R ead this section first.

S ection 2. S ta rtin g up/exiting program s and [S pectra M ana ger]

T his section outlines the procedures associated w ith op era ting the V -500W includin g starting up the spectrophotom eter, P C , W indow s, and V -500W , as w ell as exiting V -500W and W indow s and shutting dow n the spectrophotom eter. S pecific pro gram op era tions are described in subsequent sections. This section also describes the m enu that appea rs w hen you start up JA S C O [S pectra M a nager].

S ection 3. Introdu ction to qu antitative analysis and spectrum m easurem ent

T his section describes quantitative analysis and spectrum m easurem ent. This section introduces the inexperienced user to W indow s, spectrophotom etry, and V -500W operation.

S ections 4. to 9. S tandard m easurem ent program reference

T his section p rovides a reference to explain th e functions of each m ea surem ent progra m . It also explains the procedure for setting instrum ental h ardw are and self-diagnostics.

S ection 10. A pp end ix

T his section describ es how to install the so ftw are and set the serial port.

1.2 F o rew ord and N otation U sed

V -500W runs o n W indow s 95/9 8/N T 4.0/2000, so you need to be fam iliar w ith basic W indow s operations. T his m anual does not explain how to open m enus, select com m ands, or cop y files. If necessa ry, read the W indow s docum entation b efore o perating V -500W .

T he follow ing notational conventions are used throughout this m anual:

General Notation Notation Meaning [Measurement] menu [Parameters...] command

Names of menus, commands, and text boxes are enclosed in square brackets [ ], followed by a description indicating whether the function is a menu, command, text box, or other.

Names of buttons are enclosed in angular brackets < >.

Keyboard Operations Notation Meaning Shift CTRL

Names of keys found on the keyboard are enclosed in boxes.

Alt , F

Keys that are to be pressed in succession are separated by commas. In the example shown on the left, the Alt key is to be pressed and released, followed by the F key.

Shift + ®

Keys that are pressed simultaneously are linked by a plus sign. In the example shown on the left, press the ® key while holding down the Shift key.

Mouse Operations Notation Meaning Point

Move the mouse pointer to the specified item.

Click

Quickly press and release the mouse button.

Double-click

Click the mouse button twice in rapid succession.

Drag

Point to an item, click and hold down the mouse button. Move the mouse with the button held down, and release the button when the pointer is where you want it.

1.3 O verview of [S pe ctra M anager]

T he follow ing program s are registered in th e [S pectra M anager] of th e M odel V -500W as standard.

M easu rem ent program s (1) [Q uantitative m easurem ent] program

T his program creates a calib ration curve by m easuring a standard sam ple w ith know n concentration

according to the com m on quantita tive analysis m ethod and m easures an unknow n sam ple to find its concentration.

(2) [S pectrum m easurem ent] progra m

T his program obtains the U V /V IS absorptio n spectrum of a sa m ple. T he spectrum m easured by this

pro gram is autom atically transferred to the [S pectra A nalysis] progra m .

(3) [Tim e C ourse M easurem ent] program

T his program m easures the changes in a sam ple w ith tim e at a fixed w avelength. T he tim e course

data obtained by this program is transferred to the [S pectra A nalysis] program .

(4) [Fixed W avelength M easurem ent] program

T his program m easures the absorbance or transm ittance of a sam p le at a fixed w avelength. U p to

eight w avelengths can be set and m easured.

(5) [A bs/% T M eter] progra m

T his program reproduces the analog spectrophotom eter o n the C R T screen , so you ca n read the

absorbance (A bs) or transm itta nce (% T ) at an arbitrary w avelength on the m eter.

(6) [E nvironm ent] program

T his program sets the system hard w are, does self-diagnosis, sets optional accessories, and

calib rates w avelengths.

A nalysis program (1) [S pectra A nalysis] program

T his p rogram saves, prints, an d processes (difference spectrum , peak picking, sm oothin g, derivative,

vertical axis conversion, and so on) spectrum data or tim e course data.

(2) [File V iew er] progra m

Y ou u se this program to search fo r a spectrum sa ved on the d isk.

(3) [JA S C O C anvas] program

Y ou use this program to lay out and print spectra, m easurem ent para m eters, com m ents, and so on.

Y ou ca n also create d raw ings an d enter chara cters.

N ote: T his m anual describes the m easurem ent program . F or the A nalysis program , refer to the S pectra A nalysis

P rogram M anual.

2. Starting and exiting programs and [Spectra Manager]

T his section describ es how to start and exit program s and the [S pectra M anager].

2.1 S tartup

2.1.1 T urning O N the spectropho to m eter

T urn O N the pow er sw itch on the right side of the spectrophotom eter.

"Power" Switch

"Power" Lamp

F igure 2.1 S pectrophotom eter (V -53 0)

W hen the pow er is turned O N , the pow er lam p on the spectrophotom eter is lit.

T he light so urce needs about five m inutes to becom e stable. T hen m easurem ent m ay begin.

2.1.2 P C and W indow sÒ sta rtup

T urn O N the pow er sw itches for the P C and C R T. M icrosoft W indow s should start au tom atically. If necessary, refer to the M icrosoft W indow s instruction m anual

2.1.3 [S pectra M anager] startu p

(1) W hen W indo w s is started, [Jasco]-[S p ectra M a nager] also sta rts. (S ee F ig. 2.2.) T he [S pectra

M anag er] w indow app ears. (S ee F ig. 2.3.)

F igure 2.2 W indow s screen

F igure 2.3 [S pectra M anager] w ind ow

(2) In the [S pectra M anager] w indow , m ake sure th e Instrum ent in the [Instrum ents:] box is the

spectrophotom eter you are u sing. If not, click the arrow at the right side of the box and select the correct spectrophotom eter. T he w indow changes to the m enu for that spectro photom eter.

N ote: The [S pectra M anager] w indow displays the available program s for the spectrophotom eter. F ig. 2.3 show s

an exam ple of the standard program m enu. If an optional application program has been included, it w ill also appear in the [S pectra M anager] w indow . S ee S ection 2.3, [S pectra M anager] M enu for a full explanation.

(3) D ouble-click a prog ram on the m enu. T he selected program sta rts and the program w indow appears.

T he spectrophotom eter also starts autom atically, but need s about tw o m inutes to w arm up. M essag es appea r throughout the procedure. For exam ple, w hen the [S pectrum M easurem ent] pro gram is started, the [S pectrum M ea surem ent] display show n in F ig. 2.5 appears after the display show n in F ig. 2.4 appears.

N ote: S ee S ection 3 Introduction for a full description of the M easurem ent program operation.

F igure 2.4 M essage boxes during startup

N ote: C heck the follow ing if the m essage on the right in F ig. 2.4 appears.

P ow er to the spectrophotom eter is turn ed O N .

T he spectrophotom eter and P C are correctly interfaced.

T he P C environm en t (for exam ple, com m unication po rt) is correctly set.

F igure 2.5 [S pectrum M easurem ent] display

2.2 E xiting

T his section describes how to exit the [M easurem ent] or [A nalysis] program s and how to sh ut dow n the spectrophotom eter and P C .

2.2.1 E xitin g m easurem ent or spectra analysis program

E nds the [M easurem ent] or [ A nalysis] program and exits it.

(1) E xiting the [S pectra A nalysis] progra m

C lick [F ile] - [E xit]. T he [S pectra A nalysis] w indow closes and the [S pectrum M easurem ent] w indow appears.

N ote: If a spectrum has not been saved, a m essage appears to inform the operator. P ro ceed according to the

m essage. A m essage appears for each unsaved spectrum . R epeat procedure accordingly.

(2) E xiting the [S pectrum M easurem ent] progra m

C lick [M easurem ent] - [E xit]. The [S pectrum M ea surem ent] w indow closes and the [S p ectra

M anag er] w indow app ears.

(3) E xiting the [S pectra M anager] program

C lick [A pplications] - [E xit].

(4) E xiting W indow s

E xit W indow s accord ing to the W indow s U ser’s G uid e.

2.2.2 P C an d sp ectropho tom eter shutdow n

(1) Turn O F F the po w er to the P C and display. D o not forget to turn off the disp lay.

(2) C heck that the sam ple cha m ber is em pty. T hen turn O F F the pow er to the spectrophotom eter.

2.3 [S p e ctra M anager] M enu

T he [S pectra M a nager] is used to start m easurem ent, sp ectra an alysis and en vironm ent setting using the spectrophotom eter, and selecting, for starting up and shutting dow n the sp ectrophotom eter, as w ell as for setting the co m m unica tion port.

F igure 2.6 [S pectra M anager] w ind ow

F ig. 2.6 show s the standard [M easurem ent] and [A nalysis] program m enu. W hen addition al progra m s are installed, they are added to this m enu. The [A nalysis] m enu appears on the left and the [M easurem ent] m enu appears on the right. D ouble-click a pro gram to start it. If the spectrophotom eter has n ot already been started , it w ill start tog ether w ith the program .

N ote: If m ultiple instrum ent program s are installed in the P C , select one from the [Instrum ent] list.

M enu [A pplication] m enu [A nalysis] S tarts the [A nalysis] program . [M ea surem ent] S tarts the [M ea surem ent] program . [E xit] E xits the [S pectra M anager] w indow and returns to W indow s.

N ote: The progra m can also be started by double-clicking the m enu item .

[Instrum ents] m enu [S tart] Initializes the spectrophotom eter and starts com m unication. Initializa tion

takes about tw o m inutes. T his o peration is usually not necessary because the spectrop hotom eter starts autom atically w hen the [M easurem ent] pro gram is started.

[S top] S tops com m unication w ith the sp ectroph otom eter. T his o peration is

usually not necessary because com m unicatio n w ith the spectrophotom eter stops auto m atically w hen the [M easurem ent] program is exited.

[P ort S etting...] C hanges the com m unication port w ith the spectrophotom eter. [C O M 1] is

the default serial port for V -500W . S ee S ection 10.2 S etting the S erial P ort (R S -232C ).

[O ption...] Install the optio nal accessory (R efer to the accessory instruction m anual). [A bout...] D isplays the version inform ation of the control driver of the

spectrophotom eter. [H elp] m enu [C ontents] D isplays the help C ontents w indow . [S earch T o pic] D isplays the keyw o rd w indow . [A bout...] D isplays the version inform atio n o f the control program of the

spectrophotom eter. [S ystem inform ation ...] D isplays the system inform atio n.

3. Quantitative Analysis and Spectrum Measurement

T his section describes qu antitative analysis and spectrum m easurem ent. The param eters are describ ed only briefly in order to clarify th e operation flow . F ollow the procedures outlined below in order to becom e fam iliar w ith the operation of V -500W . F or m o re detailed inform ation, see the section for each program .

3.1 Q uantitative A nalysis Introduction

T he follow ing sectio ns briefly describ e the quantitative analysis program and its operation flow , follow ed by the procedure for creating calibration curves, unkno w n sam ple m easurem ent, and saving and printing results.

3.1.1 Q uantitative analysis program overview

3.1.1.1 Quantitative analysis program

T he q uantitative analysis program has the follo w ing features.

(1) T hree m ethods of analysis outlined.

1) 1-w avelength quantitative analysis. F ig. 3.1 (1). U sed for norm al solution sam ple.

2) 2-w avelength quantitative analysis. F ig. 3.1 (2). U sed for baseline correction.

3) 3-w avelength quantitative analysis. F ig. 3.1 (3). U sed for baseline correction. T he follow ing form ula is used for 3-w avelength quantitative analysis. W L1 is the w avelength and E

(1) the abso rbance at that w avelength.

DAbs E

WLWLEWLWL

WL WL

= -

-×+-×

( )

||()||(

)

| |

12331

3 2

WL 1 WL 1WL 2

D Abs

WL 1WL 2 WL 3

E(2)

E(1)

E(3)

(1) (2) (3)

1-wavelength

2-wavelength 3-wavelength

(Peak) (Base 1) (Peak) (Peak)(Base 1) (Base2)

F igure 3.1 Q uantitative analysis m ethods

(2)

T he ca libratio n curve can be selected from the m od es show n below , accord ing to the a pplica tion.

Segmented line Logistic function

Spline function

Proportional expression

Linear expression Quadratic expression

Third-order expression

F igure 3.2 C alibration curve m odes

(3) T h e operator can set w h ether to use standard m ea surem ent data (set of co ncentration/absorb ance)

to calculate the calibration curve.

3.1.1.2 Quantitative analysis operation

S tart quantitative analysis program S ee S ection 3.1.2

C reate file

S et quantitative analysis m ethod and m easurem ent param eters

S et calibration curve param eters and input standard sam p le concentration S ee S ection 3.1.3

M easu re standard sam ple blank

M easu re standard sam ples

D isplay calibration curve

M odify (check and correct) calibration curve S ee S ection 3.1.4

S ave calibration curve S ee S ection 3.1.5

M easu re unknow n sam ples S ee S ection 3.1.6

S ave results S ee S ection 3 .1.7

P rint R esults S ee S ection 3.1.8

E xit quantitative analysis program S ee S ection 3.1.9

3.1.2 P rogram startup

In the [S pectra M a nager] w indow , double-click [Q uantitative A nalysis].

T he m essage U N D E R IN IT IA LIZ A TIO N appears and m easurem ent param eters are transferred to the spectrophotom eter. W hen transfer is finished, the pro gram starts and th e follow in g w indow appears.

F igure 3.3 [Q uantitative an alysis] w indow

3.1.3 C alibratio n curve creation

(1) C lick [F ile] - [N ew ...]. T he follow ing dialog box appears.

F igure 3.4 [O pen P aram eters] dia log box

(2) C lick <N ew > to open the follow ing dialog box.

F igure 3.5 [Q uantitative M easurem ent P aram eters] dialog b ox

(3) C hanging m easurem ent para m eters procedu re

T he [Q uantitative M easurem ent P aram eters] dialog box displays the instrum ent d efau lt settings. T he follow ing param eters m ay be changed.

R esponse: F ast M ethod: 1 W avelength P eak w avelength: X X X nm

1) [R esponse] is a drop-dow n list box. C lick the arrow to the right of the box to display the full list of options. T o select [F ast] response, click [F ast].

C hange other param eters if necessary.

2) C lick the [1-w avelength] option button of the [M ethod] group to select this m ethod. T he bu tton becom es filled in [·]. N ext, use the num ber pad to input the peak w avelength of the standard sam ple

into the [P eak] text box.

(4) C lick <O K > to transfer the m easurem ent para m eters to the spectrophotom eter. W hen transfer is

finished, the [C alibrate C urve P aram eters] dialog box appears. Y ou can set the calibration curve param eters and input th e concentration of th e standard sam ple.

F igure 3.6 [C alibrate C urve P ara m eters] dialo g box

(5) C alibration curve para m eter setting

1) S et [C alib . curve] to [P ro portional]. U se the sam e procedu re as for changing param eters

2) If [S tandard blank] is know n, input tha t value to the text box. If the [S tandard bla nk] is unknow n, it w ill be m easured later. In that case, steps 2) and 3) are not necessary.

3) S elect the [E n able B lank] checkbox. T he ch eckbox is m arked w ith an [x].

(6) Inputting concentration

1) C lick the [S td#01] line of the standard data display field. The cursor m o ves to that line.

2) Input concentratio n to the [C onc.] text box of the [C alibrate D ata S etting] group. C lick <A ppend>. T he concen tration appears in th e standard data display field and the cursor m oves to the next line autom atically.

N ote: If the absorbance of the standard sam ple is know n, m easuring the standard sam ple is not necessary.

In put the absorbance, then select the [E nable C alib. D ata] checkbox. C lick < A ppend>.

3) R epeat step 2 ) as m any tim es as the num ber of standard sam ples.

4) C lick the [S td#01] line in the data display field. The cursor returns to line 1.

(7) C lick < S tart...> . T he [Q uantitative M easurem ent] dialog box opens. T he standard b lank and standa rd

sam p les are m ea sured.

N ote: The [Q uantitative M easurem ent] dialog box appears on top of the [C alibrate C urve P aram eters] dialog

box. T o view the [C alib rate C urve P aram eters] dialog box, click and drag the title bar of the [Q uantitative M easurem ent] dialog box. B oth dialog boxes are active. T he calibration curve param eters can be changed according to steps (5) and (6).

F igure 3.7 [Q uantitative M easurem ent] dialog box

(8) M easuring the standard blank

1) S elect the [B lank] option button.

2) Place the standard blank in the cell holder of the sam ple cham b er. T he cell holder is on the near-side.

Sample Chamber

F igure 3.8 S am ple cham ber

3) C lick < S tart> . T he standard blank is m easured. T he value autom atically app ears in th e [S tandard blank] text box of the [C alib rate C urve P aram eters] dialog box and the [E nable B lank] checkbox becom es selected.

N ote: W hen <O K > is clicked and the [C alibrate C urve P aram eters] dialog bo x is closed, th e standard blank

valu e is subtracted from the abso rbance value of the standard sam ple. T he standard blank and standard sam ple can be m easured in any order.

(9) S tandard sam ples m easurem ent

B efore m easuring the stand ard sam ples, check that the curso r is positioned at the first line of the standard data display field . C lick the [S td#01] line to m ove the cursor to the first line.

1) S elect the [S tandard] option button.

2) P lace standard sam ple N o. 1 in the cell holder.

3) C lick <S tart> . The standa rd sam ple is m easured. The abso rbance value appears autom atically in the standard da ta displa y field of the [C alibrate C urve P aram eters] dialog box. T he

[U se] field changes from [---] to [U se]. T he cursor autom atically m oves to the next line.

N ote: The sta ndard blank value is not subtracted from th e absorbance in the standard data display field .

4) R epeat steps 2 ) an d 3) as m any tim es as the num ber of standard sam ples.

5) A fter stand ard sam ple m ea surem ent, click <C lose> to close the [Q uantitative M easurem ent] dialog bo x.

(10) D isplaying the calibration curve

C lick < O K > in the [C alibration C urve P aram eters] dialog box. T he standard blank value is subtracted from the absorbance value of the standard sam ple an d the [C alibrate C urve] w indow o pens. A t the sam e tim e, the [M ethod Inform ation] and [D ata sheet] w indow s open.

N ote: If a calibration curve is created by clicking [M ethod] - [N ew ], only the [C a librate C urve] and [M ethod

In form ation] w indow s open.

F igure 3.9 [D ata S heet] w indow

3.1.4 C alibratio n curve m odification

C lick the title bar of the [C alibra te C urve] w indow to activate it. T he calibration curve can be confirm ed. If the calib ration curve m ust b e changed, click [M etho d] - [M odify...]. The [C alibrate C urve P aram eters] dialog box opens (S ee Fig. 3.8).

C alibration curve param eters can be changed accordingly, and then the standard sam ple can be re-m easured. D ata can be also invalidated rather than continuing w ith m ea surem ent.

N ote: The calibration curve cannot be m odified after m easuring an unknow n sam ple.

(1) R e-m easurem ent

1) M ove the curso r to the incorrect data line.

2) C lick <S tart...> to open the [Q uantitative M easu rem ent] dialog box. R epeat standard sam ple m easurem ent.

(2) Invalidating

1) M ove the curso r to the incorrect data line.

2) U nselect the [E nable C alib. D ata] ch eckbox, a nd th en click <A ppend> . T he [U se] cha nges to [---].

3.1.5 S aving qua ntitative a nalysis m ethod

S ave to disk th e qu antitative an alysis m ethod (calibration curve data) and the m easurem ent param eters.

(1) C lick [M ethod] - [S ave A s...]. T he follow ing dialog box appears.

F igure 3.10 [S ave P aram eters] dialog box

(2) Input a filenam e to the [P aram eter N am e] text box. T he filena m e m ay conta in up to 32 characters. A

m axim um of 32 calibra tion curve files m ay be input.

N ote:

C lick < C om m ent...> to open the [C om m ents] dialog box. S am ple nam e, operator, and orga nization can

be input if necessary.

(3) C lick <O K > to save the quantitative analysis m ethod onto the d isk.

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