148 Series
User’s Manual
1
148 Series
Before Use ............................................................................................ 3
Introduction ............................................................................................................3
Safety Precautions ..................................................................................................4
Parts ...................................................................................................... 5
M148 .......................................................................................................................5
Denition of Parts ..................................................................................................6
Getting Started .................................................................................... 7
Assembly .................................................................................................................7
Operation ............................................................................................. 8
Setting Up ...............................................................................................................8
Focusing ..................................................................................................................8
Adjusting e Diaphragm ...................................................................................... 8
Attaching a Camera / Changing Eyepieces ............................................................8
Setting the Stage’s Stop-Limit .................................................................................9
Adjusting Focusing Tension ...................................................................................9
Maintenance/Precautions ....................................................................................10
Table of Contents
Specications .....................................................................................11
148 Series Specications .......................................................................................11
Optional Accessories .............................................................................................12
Objectives ..............................................................................................................13
Eyepieces ...............................................................................................................13
Technical Parameters .......................................................................14
Electrical System ...................................................................................................14
Technical Terms & Concepts ................................................................................15
Common Issues ..................................................................................................... 16
Common Issues (Continued) ...............................................................................17
2
148 Series
Introduction
Congratulations on the purchase of your new AmScope microscope!
is manual is designed for the M148 series microscopes.
Please be sure to take a few minutes to familiarize yourself with the features and functions of your
new AmScope microscope.
If you’d like more information on microscopes, parts, or accessories, please visit our website at:
We highly recommend you study this manual thoroughly before operating the microscope, and
that yo ukeep it on ahnd for future reference.
Before Use
www.iScopeCorp.com
If you have additional questions or need assistance, please send us an email at:
info@amscope.com
3
148 Series
Safety Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transporation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keap it indoors in a dry
and clean place with temperateures between 32-100 degrees F (0-40 degrees C), and in
maximum relative humidity of 85%.
3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your ngerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is consident with
the voltage of your microscope.
Before Use
4
148 Series
M148
Rotating Monocular Head
Nosepiece Turret
Parts
Eyepiece
Ocu l a r Tu b e
Objectives
Stage
Illuminator Lens
Limit Stop
Stage Clips
Focusing KnobDisc Diaphragm (Disc Iris)
5
148 Series
Parts
Denition of Parts
Base Illuminator Lens
Directs the light source towards the slide
Focusing Knob
Used to bring the slide into the sight and focus
Disc Iris Diaphragm
Controls the amount of light that hits the slide from the base illuminator
Limit Stop Knob
Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective
Nosepiece
Houses the objective lenses
Rotating Monocular Head
Houses the eyepiece and optics of the microscope
6
148 Series
Getting Started
Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which
side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the
optical items. Check carefully to ensure that all parts and accessories are intact.
2. Check the packing list to ensure that you’re received all items:
- One Monocular Microscope Body & Head
- ree Objectives (4x, 10x, 40x)
- 10x Wideeld Eyepieces
- 16x Wideeld Eyepieces (for -A models only)
- 20x Wideeld Eyepieces (for -B models only)
- 25x Wideeld Eyepieces (for -C models only)
- One Dust Cover
Note: LED models do not have a spare bulb, as the LED bulbs do not need replacing.
3. Remove the microscope body from the box and remove the plastic protective covering. e body of the microscope is composed of the base, the stage, the arm, and the nosepiece.
4. Screw the objectives into the microscope nosepiece from the lowest magnication to the highest, again avoiding touching the lenses.
5. Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob on
the side of the base.
7
148 Series
Operation
Setting Up
1. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly
in place with the metal slide holders (clips) of the mechanical stage.
2. Center the specimen over the stage opening, lining it up with the light and the objective lens.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnication rst (4x objective) to locate and focus on the specimen. As you move up in magnication you may need to refocus the image a little each
time.
2. Being focusing by rst looking with one eye through the eyepiece without the diopter. Close your other eye. Use
the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.
Note: You may loosen the limit-stop knob (located on the read of the stage) in order to give yourself the full range
of motion for ne tuning the focus.
3. Once the image is clear in your eld of view, you will want to use the ne focusing knob to tune it for best results.
Note: Please be careful when moving the mechanical stage if you need to recenter the sample, or if moving the
stage very close to the objectives. e limit stop is designed to prevent impact between objective and slide, so when
it is o you will be able to damage the microscope.
Adjusting The Diaphragm
1. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the
aperture of the iris diaphragm by rotating the disc underneath the stage to the desired aperture.
Attaching a Camera / Changing Eyepieces
1. To remove the 10x eyepiece included on the microscope, you will need to remove the small metal screw on the
ocular tube just under where the eyepiece connects to the tube. To do so, you will need a 1mm athead precision
screwdriver (much like what you would use to adjust eye glasses).
2. Once unscrewed, remove the eyepiece and slide in the camera or alternate eyepieces.
8
148 Series
Operation
Setting the Stage’s Stop-Limit
1. To adjust the stop limit on the stage, unlock it by unscrewing the lower nut. e stop limit is located at the rear
of the stage.
2. Once tension is released on the nut by rotating it counter clockwise, you may adjust the upper thumb screw to
move the limit up or down as desired.
3. Lock the limit stop nut back into place aer the desired stage height is achieved.
9
148 Series
Operation
Maintenance/Precautions
- All glass surfaces must be kept clean. Fine dust on the optical surface should be blown o using a can of compressed air or gently wiped o with a so lens paper tissue/nonabraisive lint free cloth.
- Carefully wipe o oil or ngerprints on the lens surfaces using tissue moistened with a small amount of lens
cleaner (we recommend Sparkle brand optical cleaner).
- Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted
surfaces.
- Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of
our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so.
- Aer use, cover the microscope with the provided dust cover.
- Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damage.
- To change batteries with this unit, use the included allen wrench (hexagonal shaped L-tool) to undo the door hex
screw on the base. Use 3 AAA batteries with the unit.
- e unit can be used as a recharger for AAA batteries specied as rechargeable only. Please do not use standard
batteries in the unit with the charger plugged in to avoid damage.
10
148 Series
Specications
148 Series Specications
Parts Specications M148 M148A M148B M148C
WF Eyepiece
Plan Eyepiece
DIN Achromatic Objectives
Plan Objectives
45 Degree Viewing Head
Diaphragm
Illumination
Lamp
Filter
WF10X/18mm
WF10X/18mm w/ Pointer x x x x
WF10X/18mm w/ Reticle
WF16X/18mm x
WF20X/18mm x
WF25X18mm x
P5X
P10X
P16X
4X/0.10 x x x x
10X/0.25 x x x x
40X(spring)/0.65 x x x x
60X(spring)/0.85
100X(spring, oil)/1.25
4X
10X
40X(spring)
100X(spring, oil)
Binocular Sliding, 360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling
Monocular, 360 Degree Swiveling x x x x
Iris Diaphragm Disc x x x x
Halogen Light w/ Dimmer
LED Light x x x x
6V/20W
6V/30W
LED x x x x
Blue/Yellow/Green
11
148 Series
Specications
Optional Accessories
Parts Description Model # Purpose
Eyepiece 5X EP5X18 Obtaining 20x, 50x, 200x, and 500x magnication
powers
20x EP20X18 Obtaining 80x, 200x, 800x, and 2000x magnica-
tion powers
25x EP25X18 For obtaining 250x and 2500x magnication pow-
ers
10x w/ Pointer EP10X18P For easier identifying of objects
10x w/ Reticle EP10X18R For measuring objects
Objective 2X A2X For obtaining 20x and 32x magnication powers
5X A5X For obtaining 50X and 80X magnication powers
20X A20x For obtaining 200x and 320x magnication powers
60X A60X For obtaining 600x and 960x magnication powers
Plan 4X PA4X For obtaining higher clarify in images
Plan 10X PA10X For obtaining higher clarify in images
Plan 40X PA40X For obtaining higher clarify in images
Plan 100X PA100X For obtaining higher clarify in images
Camera CMOS Digital MU035 (350k)
MU130 (1.3mp)
MU300 (3mp)
MU500 (5mp)
MU800 (8mp)
MU900 (9mp)
MU1000 (10mp)
Calibration Micrometer MR400 To calibrate the camera soware for on screen
CCD TV/Video CCD-NP To view live display on a television (RCA)
Case Aluminum Case AC-B100 For carrying microscope around safely
To capture images, video, or view live display on a
computer (PC/Mac OS X)
measurements
12
148 Series
Objectives
Specications
Type Magnication Numerical Aperture
DIN Achromatic
Objective (195mm)
Plan Objective
(195mm)
(N.A.)
4X A2X Air 45 Red
10X A5X Air 45 Yel low
40X A20x Air 45 Light Blue
60X A60X Air 45 Deep Blue
100X A100X Cedar Oil 45 White
Plan 4X PA4X Air 45 Red
Plan 10X PA10X Air 45 Ye l low
Plan 40X PA40X Air 45 Light Blue
Plan 100X PA100X Cedar Oil 45 White
Medium Parfocal Distance (mm) Magnication Marks
(Color Ring)
Eyepieces
Type Wideeld Eyepiece
Medium
Magnication 10X 15X 20X 5X 10X 16X
Field of View Φ18 Φ13 Φ11 Φ18 Φ18 Φ15
Plan Eyepiece
13
148 Series
Technical Parameters
Electrical System
ere are two options for eletrical systems for this series of microscope. e light source is an LED system.
1. 220V~240V power supply: 220V~240V ±10%, 50Hz
is electrical system is CE and GS certied
2. 100V~120V power supply: 100V~120V ±10%, 60Hz
is electrical system is UL certied.
All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent
on which unit is purchased.
is unit is also capable of using batteries and charging rechargable batteries. It uses 3 AAA batteries. When
plugged in with batteries in the unit, the batteries will be charged.
Note: Please do not use the recharging feature with standard batteries, as damage to your unit can occur.
14
148 Series
Technical Parameters
Technical Terms & Concepts
Total Magnication
Total magnication of a microscope is calculated by the magnication of the objective multiplied by the
magnication of the eyepieces.
-Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnication
Field of View
Linear eld of view of the eyepiece divided by the magnication of the objective
Numerical Aperture (N.A)
Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the fetaures of the objective and condenser’s image quality and resolution. e “n” is a refractive index of the medium
(air or immersion cedar oil) between the omjective lens and the specimen. e “α” is 1/2 of the angle between the
aperture on the objective and path of light. e larger the N.A, the higher the resolution of the objective (and better
quality of the image).
Object to Primary Image Distance
e distance between the object plane and the primary image plane. e conjugate distance is xed.
Mechanical Tube Length
e distane between the objective shoulder and the ocular shoulder
15
148 Series
Troubleshooting
Common Issues
Symptom Cause Remedy
OPTICAL ISSUES
One side of the eld
of view is darker
Obstructions are
observed in the eld
of view
Unclear Image ere is no cover slip on the slide Add a cover slip. e objectives are designed for use
One side of the eld
of view is dark or the
image moves while
focusing
e eld of view is not
bright enough
e nosepiece if misaligned Turn the nosepiece until it clicks into place
Stains or dust has accumulated on the objective,
eyepieces, or base lens
Stains, dust, or dirt has accumulated on the specimen
Stains, dust, or dirt have accumulated on the lens Clean the lens
e cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm
e aperture is not open to an appropriate diameter
Stain or dust has accumulated on the lens in the
inlet of the head
e specimen slide is not xed Secure the slide to the stage with clips
e nosepiece is not in the right position Turn the nosepiece until it clicks into place
e iris disc diaphragm is not big enough Rotate the iris disc diaphragm to allow more light
Stains, dust, or dirt has accumulated on the condenser, objective, eyepieces, or base lens
Clean all lenses with lens cleaner or a lint free nonabraisive cloth
Clean the slide or use a new specimen if sample is
destroyed
with a 0.17mm cover slip, so it is a requirement to
use one for proper images.
thickness slip
Adjust the aperture to have the light just larger than
the size of the condenser
Clean the lens with lens cleaner or a nonabraisive
lint free cloth, as well as spray with compressed air
to travel
oroughly clean tall lenses with lens cleaner or a
lint free nonabraisive cloth
16
148 Series
Troubleshooting
Common Issues (Continued)
Symptom Cause Remedy
MECHANICAL ISSUES
e objective touches the
cover slip
Unable to move the slide
smoothly
Focus knob does not turn e tension knob is too tight Loosen it by adjusting the tension ring inside the coarse focus
Stage declines by itself e tension knob is too lose Tighten it by adjusting the tension ring inside the coarse focus
e focusing knob won’t
raise the stage
e bulb/light source
ickers
e microscope does not
light up
e cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm thickness slip
e limit-stop is set too high or not engaged Be careful to avoid contact between objective and the slide when
the limit stop is not engaged. To reengage, focus the sample, then
lock the limit stop into place to set max height at a safe but usable distance.
e slide is not secured correctly Adjust the slide to use the stage clips and secure the sample
e mechanical stage is not properly secured Tighten the mechanical stage screws to better secure the stage
knob counterclockwise (close to the arm of the microscope on
the le of the microscope)
knob clockwise (close to the arm of the microscope on the le
of the microscope)
Limit-stop is engaged Disengage the limit stop on the rear of the stage of the micro-
scope
ELECTRICAL ISSUES
e bulb is close to burning out Please contact us in regards to this issue. LED lights do not burn
out, so there may be another electrical issue
e microscope is unplugged Insert the plug into the wall socket to achieve electrical illumina-
tion
17
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