I-Scope DN117D-LED User Manual

DN117D-LED

I-SCOPE OPTICS JAPAN

Digital Microscope

Model Number

DN117D-LED

User Manual

DN117D-LED

I-SCOPE OPTICS JAPAN

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CONTENT

User Notice ............................................................................................ 2

1. Components ....................................................................................... 4

2.Assembly ............................................................................................ 5

2.1 Assembly Diagram ..................................................................... 5

2.2 Assembly Steps ........................................................................... 6

3.Adjustment & Operation .................................................................. 10

3.1 Adjustment Sets ........................................................................ 10

3.2 Operation .................................................................................. 11

4. Specification Table .......................................................................... 14

4.1 Main specifications .................................................................. 14

4.2 Eyepieces and Objectives ......................................................... 14

4.3 Total Magnification .................................................................. 15

5．Outfit .............................................................................................. 15

6．Troubleshooting Guide .................................................................. 16

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User Notice

Ⅰ. Safety Notes

1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being damaged.

2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy

shaking environment. Make sure the stage is smooth, horizontal and firm enough.

3. When moving the instrument, please use two hands to grip with the two sides of the microscope

body.

4. When running, the lamp house and nearby parts will be very hot. Please ensure there is enough

cooling room for them.

5. Make sure the instrument is earthed, to avoid lighting strike.

6. For safety, be sure the main switch is in “O”(off) state before replacing the halogen lamp or the

fuse, then cut off the power, and do the operation after the lamp bulb and the lamp house
completely cool down.( Specified: LED 3W)

7. Check the input voltage: be sure the input voltage which signed in the back of the microscope is

consistent with the power supply voltage, or it will bring a serious damage to the instrument.

8. Use the factory supplied power cord, please.

II. Maintenance

1. All the lenses have been well checked and adjusted. It is forbidden to disassemble them yourself.

2. The nosepiece and coarse/fine focus unit have a compact and precise frame; please don’t

disassemble them as possibly as you can.

3. Keep the instrument clean, wipe dust regularly, and be attention to avoid contaminating the

optical elements especially.

4. The contaminations on the prism, as finger mark and oil, could be gently wiped with a piece of

soft cloth or tissue paper, gauze which has been immersed in pure alcohol or ether. (Note that

the alcohol and ether are highly flammable, do keep them away from the fire or potential
sources of electrical sparks, and use them in a drafty room as possible as you can.)

5. Do not attempt to use organic solvents to clean the microscope components other than the glass

components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral
detergent.

6. When using, if the microscope is splashed by liquid, cut off the power at once, and wipe up the

moisture.

7. Do not disassemble any parts of the microscope, which will affect the function or decline the

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performance of the microscope.

8. Place the instrument in a cool, dry position. When not using the microscope, keep it covered

with a dust cover. Make sure the lamp socket is cool before covering the microscope.

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1. Components

Digital Biological Microscope

Eyepieces

Digital viewing head

Nosepiece

Objectives

Main body

Condenser

Collector

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2.Assembly

2.1 Assembly Diagram

The following figure shows the installation sequence of the components. The number in the figure

shows the assembly steps.

 Before installing, be sure every components is clean, do not score any parts or glass surface.
 Keep well with hexagon wrench provided. When replacing the components, you will need it

again.

③

Main body

Objectives

Digital viewing head
Eyepieces

④ ① ②

Power cord

USB data wire

⑤

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2.2 Assembly Steps

2.2.1 Installing digital viewing head
（Fig.1、2）

Insert the digital viewing head into the
microscope head, turn into the right position,
then screw down the bolt①to fix it.

2.2.2 Installing the eyepieces（Fig.3、
Fig.4）

Insert the eyepieces into the eyepiece tube
until they are against each other as shown in
Fig.4.

NOTE:

Operation Conditions：
1． Temperature： 0℃ ～40℃ ,Maximum
Relative Humidity：85%.
2．High Temperature：High Temperature
and humidity will result in a mildewing,
dew and even ruinous instrument.
3．Avoid placing the instrument in a dusty
environment. When ending your
microscope operation, please cover it with
the dust cap.
4．Lay the microscope in a plan and stable
position, please.

Fig.1

Fig.3

①

Fig.2

Fig.4

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②

①

Fig.6

Fig.5

2.2.3 Installing objectives (Fig.5& 6）

1. Adjusting the coarse focus knob until the

support device of the mechanical stage
reaches its low limit position.

2. Screw the lowest magnification objective

into the nosepiece from the left or the right
side, then revolve the nosepiece clockwise
and mount other objectives by the sequence
of low to high magnification

 Installing objective this way will make the

change of magnification to be easier during
using.

 Clean the objectives regularly, for lens is

susceptible to dust.

 When operating, use 10×magnification

objective to search and focus specimen
firstly, then replace with higher
magnification objective if necessary.

 When replacing the objective, slowly turn

the nosepiece until you hear “clicked”,
which means the objective is in the
required position—center of the light
path.

2.2.4 Installing the color filters (Fig.7)

1. Turn the condenser bracket① out at the

direction of arrow in Fig.7

2．Put the required filters② into the holder on

the bracket, and then turn the bracket back
to the right position.

★ Baby blue and green filters are available in

standard outfit.

Fig.7

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③ ②

⑥

④ ⑤

2.2.5 Connecting the power cord

(Fig.8, 9,10)

★ The cable and cords are vulnerable when

bent or twisted, never subject the power

cord to excessive force.

1. Turn the main switch① to “O” (off) state

before connecting the power cord.

2. Insert the power plugs② into the power jack③

of the microscope; make sure the connection is

well.

3. Plug the power cord ④ into the power supply

receptacle safely. Make sure the connection is

well.

★ Do use the supplied power cord all the

time. If it lost or damaged, select the same

standard cord, please.

★ Either 110V or 220V can be selected as

the input voltage of this microscope. (The
input voltage has been preset in the
microscope before leaving factory.

2.2.6 Replacing the Fuse（Fig.8、9、10）

Do remember to set the main switch① to

the state of “O” (OFF) and unplug the power
cord ②before replacing the fuse. Scratch the
fuse holder⑤ out from the power socket③
on the microscope, replace with a new fuse in
the holder, then press the fuse holder back
again.

★ There is a spare fuse in the fuse holder.

★ For 220V input voltage，use fuse of rating

(250V500mA).

★ For 110V input voltage, use fuse of rating

(250V1A).

Fig.8

①

Fig.9

Fig.10

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②
①

④

.

⑥
⑤

2.2.7 Installing and replacing the lamp
（Fig.11,12,13）

 There are two types of illuminator available
for this microscope, one type is halogen lamp
6V20W, the other is 3W LED. During use or

just after using, the lamp house and nearby parts
will be very hot. Please set the main switch to

“O” (off) state before replacing, and make sure

the bulb, the lamp room and periphery are all cool
enough to carry no burn. Then, you can do your
replacing.

1. Loose the bolt①and open the window② on

the bottom of the microscope base with

“一”type screwdriver.

2. Pull out the old bulb③ ,hold the new bulb

after you wrap it with gauze or other

protection materials and insert its pin as

deeply as possible into the jack in the lamp

holder.

4. Close the window and tighten the bolt①.

 Please insert the bulb gently, or it will
be damaged by excessive extrusion.

 Do not touch the halogen bulb with

bare hands. It will shorten the service life

or cause it to burst. If you leave

fingerprints on the surface carelessly,

clean it with a piece of dry soft cloth.

When replacing LED ：

1. Generally, the LED has long service life and

is not easy to damage, if unfortunately it
damaged, purchase a new one from the
supplier.

2. Remove the base plate⑥ from the bottom

side of the microscope base with screw
driver ,loose the bolt⑤ to take the old LED
off, replace with a new one .

3. Put the new LED unit back onto the bracket

with the bolt⑤ and the base plate⑥onto the

bottom side of the microscope.

When you take down the base plate,

please do it gently and slowly, to avoid

damaging internal electrical wires.

Fig.11

③

Fig.13

Fig.12

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3．Adjustment & Operation

3.1 Adjustment Sets（Fig.14,Fig.15）

Main switch

Brightness Adjustment Knob

Fig.15

Tension Adjustment Collar

Right Coarse Focus Knob

Right Fine Focus Knob

Left Fine Focus Knob

Left Coarse Focus Knob

Condenser Adjustment Knob

Aperture Diaphragm Adjustment Lever

Longitudinal Adjustment Knob

Transversal Adjustment Knob

Diopter Ring

Interpupillar Distance Indicator

Fig.14

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3.2 Operation

Spanner

Fig.19

Fig.17

③

④

①

②

Fig.16

Fig.18

①

③

②

3.2.1 Adjusting the brightness（Fig.16）

1. Connect the power, turn on the main

switch①(shown in the figure)which on the

bottom side of the base to “－”(on).

2. Turning the brightness adjustment knob ②

clockwise, the voltage decline, and the

brightness weaken; Whereas turning at the

opposite direction, the voltage raise, and the

brightness strengthen.

 Using the microscope at a lower voltage
can prolong the service life of the bulb.

3.2.2 Placing the specimen（Fig.17）

1. Place the specimen③ on the center of the stage,

and then hold it with the specimen holder④.

2. Turn the transversal and longitudinal adjustment

knobs which on the mechanical ruler to move the

specimen onto the required position.

★ Be careful when changing the objective. If

you finish the observation with the short

working distance objective, and want to

change another one, be careful of not letting

the objective touch the specimen.

3.3.3 Focusing the specimen(Fig.18、19）

1. Focus the specimen with 10X objective. To avoid

the objective touching the specimen during
focusing, you should raise the mechanical stage to
let the specimen close to the objective at first, then
slowly dispart them to bring the specimen to focus.

2. Turn the coarse focus knob ①conversely to lower

the specimen and search images in the 10×ocular
simultaneously, and then use the fine knob② to
make focus. After that, you can replace with other
magnification objectives safely, and focus without
the risk of damaging the specimen.

● The tight tension of the coarse focus knob has

already been adjusted before leaving factory.

If loosen (e.g. the stage slip down by its

weight), please screw the intention adjustment

collar③ to the right position by the supplied

spanner.

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3.3.4 Condenser Adjustment（Fig.20）

Turn the condenser focus knob to move
the condenser up and down. Raise the
condenser when using the high
magnification objective, and descend it
when using the low magnification one.

 The condenser and the objective are

coaxial. It has been adjusted well before
leaving factory, so the user needn’t to
adjust them by self (the distance between
the top of the condenser and the stage
should be in the range of

0.03mm~0.4mm.)

 The highest position of the condenser has

been adjusted too. It also needn’t any
user’s operation.

3.3.5 Aperture Iris Diaphragm
Adjustment（Fig.20,21）

Turn the aperture iris diaphragm lever①

to adjust the aperture iris diaphragm.

● Generally, setting the aperture iris

diaphragm to 70- 80% of the N.A. of the
objective in use will provide an image
with good contrast.

● If the size of the aperture diaphragm

minified, the brightness and the
resolution declined, while the contrast
and the depth of field increased; In
other words, if the size largen, the
brightness and the resolution improved,
but the contrast and the depth of field
declined.

● Generally, setting the size of the

condenser aperture diaphragm at
70%～80% of the numerical aperture,
you can obtain a clear image with
enough contrast. If the open of the
aperture diaphragm is too small, the

resolution were very low, so please don’t

minify the aperture below 60% of the

objective’s numerical aperture unless in

a special case, for instance, observating
an almost transparent specimen.

①

Fig.20

Magnification：10×
N.A.：0.25

Fig.21

Specimen

Image of Aperture Iris Diaphragm

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● The numerical aperture is marked on the objective. For example, the mark “ 10/0.25 ” means the
magnification is 10×，and the numerical aperture is 0.25.

● If you want to observe the image of the aperture iris diaphragm, remove one eyepiece and look
through the tube. You will see a dark circle encroaching on the bottom of the tube.

3.3.6 Adjusting the Interpupillary
Distance（Fig.22）

The interpupillary distance range:

48mm～75mm。
When observing with two eyes, hold on the
left and right prism holders, turn them around
the axis to adjust the interpupillary distance
until the left and right fields of view coincide
completely, as shown in Fig.22.

3.3.7 Adjusting the diopter（Fig.23）

1. Looking through the right ocular with your right
eye, revolve the coarse and fine focusing
adjustment knob to focus on the specimen.

2. Then look through the left ocular with your left
eye. If the image is not sharp, turn only the left
diopter adjustment ring① to focus on the
specimen please.

★ The diopter range of the eyepiece is ±5
diopter. The number aligned to the line on the
viewing head is the diopter in use.

Fig.22

Fig.23

①

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4. Specification Table

4.1 Main specifications

Mechanical Tube
Length

160mm

Viewing Head

Compensation free binocular head, , Inclined at 30°,Interpupilary Distance 48-75
mm

Eyepiece

Field of view: φ18mm

Nosepiece

Backward Quadruple Nosepiece

Objective

Achromatic objectives 4×，10×，40×、100×

Focusing

Coaxial coarse and fine focusing knob; the minimum division of fine
focusing:0.004mm; focusing adjustment range: 24mm

Condenser

Abbe Condenser，NA=1.2 with iris diaphragm

Stage

Double Layers Mechanical Stage 132mm×142mm，Moving Range 74×40mm

Illumination

Halogen Lamp 6V20W or LED 3W

4.2 Eyepieces and Objectives

1. Objectives

Magnification

Numerical

Aperture
（NA）

Thickness of

glass slide

(mm)

Focal length

（mm）

Working

Distance（mm）

Type

4×

0.10

0.17

31.05

18

Dry

10×

0.25

0.17

17.13

6.5

Dry

40×

0.65

0.17

4.65

0.53

Dry

100×

1.25

0.17

2.906

0.13

Oil

2. Eyepieces

Category

Magnification

Focal length

f（mm）

Field of view（mm）

Plan eyepiece

10×

24.95

Φ18

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4.3 Total Magnification

Eyepiece

10×

10×

10×

10×

Objective

4×

10×

40×

100×

Total

Magnification

40×

100×

400×

1000×

5．Outfit

Component Name

Specification

Quantity

Standard

Outfit

Main body

Main Standard

1 〇 Double Layers Mechanical Stage

1 〇 Condenser Holder

1

〇

Viewing Head

Compensation free digital binocular head

1

〇

USB data wire(2 meters)

1

〇

Condenser

Abbe condenser for bright field with iris

diaphragm

NA=1.2

1

〇

Nosepiece

Quadruple

1

〇

Illumination

Halogen Lamp 6V20W (or LED 3W)

1

〇

Spare lamp （6V20W Halogen lamp ）

2

〇

Spare fuse ( 50T250V2A or 500mA)

1

〇

Eyepieces

10×Plan Eyepieces

2

〇

Objectives

Achromatic objective 4×

1

〇

Achromatic objective 10×

1

〇

Achromatic objective 40×

1

〇

Achromatic objective 100×（oil、spring）

1

〇

Filter

Baby Blue, Green

1 ea.

〇

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6．Troubleshooting Guide

1. Optical system

TROUBLE

CAUSE

SOLUTION

The edge of the
field of view is dark
or the brightness is
not uniform

The nosepiece is not in the located position (objective and
light path not coaxial)

Locate the nosepiece
properly where it clicks

The image of filament is not centered

Center the filament

A lens (the objective, condenser, eyepiece or collector) is
dirty.

Clean it thoroughly

Find dust and stain
in the field of view

There are stains on the lens（including condenser,
objective, eyepiece and collector）

Clean it up
There are stains on the specimen

Clean it up

The position of the condenser is too low

Loosen the condenser’s

locking bolt, adjust the
condenser to the right
position

The image is
defocused ( low
resolution \
contrast)

There is no cover slip on the specimen

Add coverslip

The cover slip is too thick or too thin

Use the standard
coverslip ( 0.17mm )

The specimen is placed inversely

Reversal it back

There was oil on the dry objective(easily happened in
40X objective)

Clean it up

There are stains on the lens（including condenser,
objective, eyepiece and collector）

Clean it up

didn’t use oil for the oil objective

Use immerse oil

There was bleb in the oil

Eliminate the bleb

Use a unsuitable oil

Change to the specified
one

The size of the aperture diaphragm is too big

Minify it

There are stains on the incident lens of the binocular tube

Clean it up

The size of the aperture diaphragm is too small

Open it up

The position of the condenser is too low

Adjust the position

One side of the
image is dark

The condenser is not in the center of the field of view\the
condenser inclines

Install the condenser
again and adjust the
center carefully by
centering the bolt

The nosepiece is not in the right position

Turning it until it reach
the “clicked” position

The specimen is floating

Fix it

The image shift
during focusing

The specimen slips on the stage

Fix it

The nosepiece is not in the right position

Turn it to the
“ clicked ”position

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The image is a little
yellow

Not use the blue color filter

Use the blue filter

The brightness is
not enough

The size of the aperture diaphragm is too small

Adjust again

The position of the condenser is too low

Adjust the position

There are stains on the lens（including condenser,
objective, eyepiece and collector）

Clean it up

2. Mechanical system

TROUBLE

CAUSE

SOLUTION

The image can not
focus when using high
magnification
objective

The specimen is placed inversely
The coverslip is too thick

Turn inversely
Use the standard coverslip（0.17
mm）

The objective touch
the specimen when
changed from low
magnification to the
higher magnification

The specimen is placed inversely
The coverslip is too thick

Turn inversely
Use the standard coverslip（0.17
mm）

The specimen is not
easy to move

The specimen holder is not fixed

Fix it

The binocular image is
not coincident

The interpupillar distance is not correct

Adjust it

Eyes are too tired
No diopter adjustment

Adjust the diopter correctly

The brightness is not suitable

Adjust the voltage of the lamp

3. Electrical system

TROUBLE

CAUSE

SOLUTION

The lamp can’t light
when the switch is
turned on
No power

Check the connection of the
power cord

The bulb is not inserted

Insert it correctly

The bulb burns out

Replace it

The lamp burns out
suddenly

Use a substandard lamp
The voltage is too high

Use the specified lamp to replace,
if the problem is not solved,
contact with the service department

The brightness is not
enough

Use a substandard lamp
The voltage is too low

Use the specified lamp
increase the voltage

The bulb flickers or
the brightness is
vertiginous

The bulb is going to burn out

Replace it

The bulb is not entirely inserted into the
holder

Check and insert it again

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