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3 Primary Tube Centrifuge
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Operator’s Manual
Division of IRIS International, Inc.
Operator’s Manual
Express 3 Primary Tube Centrifuge
Model Number M502-22
For in-vitro diagnostic use
Product Number
SSX3 - Express 3 for 100-240 VAC, 50/60 Hz
StatSpin and Iris are registered trademarks of IRIS International, Inc.
Copyright 2006
Printed in U.S.A.
Table of Contents
How to use this manual…………………………………………..……….………… ii
Section 1 ……………………………………………………………..……….……….. 1
Unpacking and Installation ..………………………………………………………...1
Inspect Packaging ………………………………………………………………...………................... 1
Verify Contents ………………………………………………………………...………. ........................ 1
Install ………………………………………………………………...………. ............................................ 1
Connect Power ………………………………………………………………...………. ........................ 1
Section 2 ……………………………………………………………..……….……….. 2
System Overview ……………………………………………………………………. .2
Principle and Intended Use ………………………………………………………………...……… 2
Symbols and Definitions ………………………………………………………... 2
Operator Controls ………………………………………………………………...……….................... 3
Error Indicators …………………………………………………………………… 3
Accessories………………………………………………………………………... 4
Section 3 ……………………………………………………………………………….. 5
Operating Instructions ……………………………………………………………… 5
Opening and Closing the Cover ………………………………………………………………… 5
Removing and Installing the Rotor ……………………………………………………………… 5
Spinning the Sample …………………………………………………………………………………. 6
Section 4 ………………………………………………………………………………. 8
Maintenance ….……...…………………………………………………………..……. 8
Overview ………………………………………………………………………………………………..… ... 8
Cleaning …………………………………………………………………………………………….…….... 8
Checking the Rotor Speed………………………………………………………………….…… ..... 8
Troubleshooting …………………………………………………………………………………….…… 9
Service ………………………………………………………………………………….…… .................... 10
Section 5 …………………………………………………………………………… .. 11
Appendix …..….……...…………………………………………………………..… ..11
Specifications………………………………………………………………………………………….… 11
References………………………………………………………………………………………………. 12
Warranty………………………………………………………………………………….… ...................13
i
How to use this manual
This manual along with information contained on product labels and in package inserts should
provide you with all the information you need to operate and maintain the Express 3.
Notes appear in italics to highlight information.
When the information requires special attention, a caution symbol appears next to the
italicized text.
Please pay close attention to the instructions that accompany the notes and symbols as well as the
standard laboratory practices outlined by your facility and local regulatory agencies. The table below
lists all the CAUTIONS and WARNINGS for the Express 3.
WARNING – Do not expose the Express 3 or its rotor to strong or concentrated
acids, bases or oxidizing agents, aromatic or halogenated hydrocarbons, esters or
ketones.
WARNING – Do not operate or store the Express 3 or its rotor below the 2oC
minimum operating temperature. At extreme low temperature (below -18
rotor material is subject to cracking under impact stress (such as dropping onto a
hard surface).
WARNING - Do not use the supplied line cord outside of North America. Use a
power cord with a grounded male connector suitable for the power outlet being
used, which is rated at 3.0 Amps or more and has an IEC320/CEE22 female
connector to attach to the centrifuge.
CAUTION – Never operate the Express 3 without the rotor locked securely in place.
Failure to properly install and secure the rotor may result in damage to the
centrifuge not covered by warranty.
CAUTION - Replace Tube Insert immediately if a tube breaks during centrifugation.
o
C) the
CAUTION - Replace tubes used for rotor balancing after 10 spin cycles to reduce
chances of breakage due to the repeated stress of centrifugation.
CAUTION- The rotor (RTX8) has a finite lifespan that is dependant on usage.
Cracks or swelling that develop indicate the rotor should be replaced immediately.
CAUTION - The cover interlock by-pass is for emergency use only. Misuse may
expose the operator to unsafe conditions.
CAUTION – Do not spray cleaning solutions directly onto the centrifuge bowl or
housing; overspray could reach the motor bearings or internal circuitry.
Subsequent damage may not be covered by warranty.
Please use the system as intended. Improper use of the Express 3 Primary Tube Centrifuge and/or
its accessories may cause damage to the system, inaccurate results, or potentially nullify warranties.
Do not operate or store instrument beyond specified temperatures.
CAUTION – Unplug the Express 3 from the wall outlet before cleaning or
performing maintenance.
CAUTION – Follow Universal Precautions with all biological specimens, regardless
of whether the specimen is known to contain an infectious agent. (See references)
ii
Section 1
Unpacking and Installation
Inspect Packaging
The Express 3 and its accessories are delivered in a single carton. If the centrifuge or accessories have
been damaged in transport, please inform your carrier immediately.
NOTE: Save the shipping carton and packing materials to facilitate return should service be required.
Verify Contents
The package contains:
One Express 3 centrifuge (Model No. M502-22)
One centrifuge rotor (Product No. RTX8)
Eight rotor inserts for 13x75 mm tubes (Product No. SV08)
Eight rotor inserts for 16x100 mm tubes (Product No. SV07)
One grounded line cord (for use in North America only)
One Operator’s Manual
One manual latch release tool
One Setting Guide
One 5/16 inch hex key tool
Install
o Place the Express 3 on a solid level surface suitable for operation of laboratory instrumentation.
o Maintain a 30 cm clearance around the centrifuge for ventilation and safety.
o Position the Express 3 out of direct sunlight and away from sources of intense heat or cold.
o See the Appendix for the acceptable range of operating temperature and humidity.
o Remove the foam shipping retainer around the rotor before operating the centrifuge.
Connect Power
o Plug the centrifuge into a grounded outlet supplying 100-240 V @ 50-60 Hz.
o Turn on the power switch (located on the rear panel, above the line cord connector).
o When the instrument completes the power-on check it will give a two-beep ready signal. The two-
minute green indicator will illuminate. Press the stop button to release the latch and open cover.
WARNING - Do not use the supplied line cord outside of North America. Use a power cord
with a grounded male connector suitable for the power outlet being used, which is rated at 3.0
Amps or more and has an IEC320/CEE22 female connector to attach to the centrifuge.
1
Principle and Intended Use
IVD
For in-vitro diagnostic use to produce the rapid separation of whole blood contained in original collection
tubes.
The Express 3 is a high-speed bench top centrifuge used to rapidly separate blood components in the
original sample collection tubes.
The eight-place 30° angle rotor included with the centrifuge produces rapid separation while minimizing
potential for re-suspension of cells. The operator selects a fixed spin time of 2, 3, or 5 minutes. The
rotation speed is fixed at 7200 rpm for the 2 and 3 minute cycles, producing a centrifugal force of 4440 xg
at a rotor radius of 7.65 cm.
The 5 minute cycle generates 2685 xg at 5600 rpm. This setting is intended for use with large glass
tubes (7mL and 10 mL), which require lower g-forces. Five minutes at 5600 rpm produces the same
degree of separation achieved with three minutes at 7200 rpm.
Symbols and Definitions
REF
Product/Reference
Number
Section 2
System Overview
Indicates the Iris Sample Processing product/catalog
number
Caution Statement of caution/warning: Read carefully.
IVD
S N
Temperature
limitation
EC Representative European Community Authorized Representative
For in vitro
diagnostic use
Non-sterile Indicates a non-sterile product
Serial Number Indicates instrument serial number
Indicates storage or operating temperature
restrictions
Indicates for use only as in-vitro diagnostic device
Consult
Instructions
Refers operator to the instruction manual for
additional information
2
Operator Controls
Start The Start button initiates the selected cycle.
Error/Service
light flashing
2 min
3 min
5 min
Error Indicators
Express 3 error conditions are signaled by a flashing red Error/Service indicator on the control panel.
The cause of the error condition can be identified by the combination of green cycle time indicators
that are also flashing.
Stop/Open
2 Minute Cycle
3 Minute Cycle
5 Minute Cycle
Error/Service
out-of-balance
The Stop/Open button interrupts the cycle and
releases the cover when the rotor has stopped.
The 2 min button selects a cycle of two minutes at
7200 rpm.
The 3 min button selects a cycle of three minutes at
7200 rpm.
The 5 min button selects a cycle of five minutes at
5600 rpm.
The red indicator with an adjacent wrench symbol
flashes to signal an error condition or remains
continuously illuminated when service is needed.
Rotor failed to
reach full speed
in 30 seconds.
Unable to
maintain rotor
speed
Rotor
Cover not
latched
Locked rotor
Rotor
over-speed
3
Accessories
Product No. Description
SV02 Inserts for 3 mL tubes (10.25 x 64 mm) 4/pk.
SV03 Inserts for 2 mL tubes (10.25 x 47 mm or BD Microtainers) 4/pk.
SV04 Inserts for 1.5-2 mL Microtubes (e.g. LipoClear) 4/pk.
SV05 Inserts for 2 mL tubes (BD Microtainers) 4/pk.
SV06 Inserts for 7 mL tubes (16 x 75 mm) 8/pk.
SV07 Inserts for 10 mL tubes (16 x 100 mm) 8/pk. Included with centrifuge
SV08 Inserts for 5 mL tubes (13 x 75 mm) 8/pk. Included with centrifuge
SV09 Inserts for 7 mL tubes (13 x 100 mm) 8/pk.
DL02 Disposable bowl liners 3/pk.
RTX8 8 place rotor. Included with centrifuge
4
Section 3
Operating Instructions
Opening and Closing the Cover
An electronic operated cover interlock mechanism prevents operation if the cover is not completely
closed and locked, and prevents the cover from opening when the rotor is turning. An operating
cycle can be initiated only if the cover latch is engaged.
Cover Interlock By-pass
In case of power failure or malfunction, the Stop/Open button may not release the cover. To recover
samples in the rotor, the cover interlock mechanism can be released manually by inserting the
manual latch release tool supplied with the instrument into the small hole located in the front of the
housing. Push the tool inward about one inch (25 mm) to release the latch.
CAUTION - The cover interlock by-pass is for emergency use only. Misuse may
expose the operator to unsafe conditions.
Removing and Installing the Rotor
The Express 3 is shipped with the rotor installed and supported by a shipping retainer. To prevent
possible damage to the centrifuge’s motor mount, it is important to remove the rotor whenever the
centrifuge is moved or shipped to another location.
CAUTION - The rotor (RTX8) has a finite lifespan that is dependant on usage.
Cracks or swelling are signs of fatigue, replace immediately.
Before operating the centrifuge, ensure that retaining nut has been tightened completely.
To remove the rotor:
1. Remove the tube inserts from the rotor.
2. Loosen and unscrew the retaining nut using the 5/16 inch hex key tool.
3. Remove the retaining nut and lift the rotor off the drive shaft.
To re-install rotor:
Note that there are two drive pins adjacent to the drive shaft on the rotor mount. These pins mate
with the corresponding holes on the bottom of the rotor.
1. Rotate the rotor so that wells 5 and 6 are aligned with the drive pins.
5
2. Slide the rotor onto the shaft and push down gently to seat the drive pins in the holes.
3. Place the retaining nut on the shaft and tighten securely using the 5/16 inch hex key.
You will not be able to tighten the retaining nut if the drive pins are not properly aligned. If this
occurs lift the rotor off and repeat the process until it is seated.
Spinning the Sample
CAUTION - Never operate the Express 3 without the rotor locked securely in place. Failure to
properly install and secure the rotor may result in damage to the centrifuge not covered by warranty.
Loading
1. Ensure the appropriate tube inserts are installed. The Express 3 centrifuge is provided with
eight tube inserts for 13 x 75 mm tubes (Product No. SV08) and eight tube inserts for 16 x 100
mm tubes (Product No. SV07). Other sizes are available (See accessories).
2. The rotor must be properly balanced to ensure smooth operation. DO NOT spin a single tube.
3. Use the same size and style tubes in opposite positions. Balance liquid in tubes to within 0.5 mL.
CAUTION - Replace Tube Insert immediately if a tube breaks during centrifugation.
CAUTION - Replace tubes used for rotor balancing after 10 spin cycles to reduce chances of
breakage due to the repeated stress of centrifugation.
4. Close and latch the centrifuge cover.
5. Select the spin time by pressing the appropriate cycle time button.
6. Press Start button.
Cycle Settings
The operator should select spin time settings to achieve optimum results for specific applications.
The following are general guidelines:
Setting Speed RCF
2 min Produces high quality plasma/serum from whole
blood for chemistry, coagulation and cardiac
testing.
3 min Produces high quality plasma/serum in gel tubes
and large plastic collection tubes from whole blood
for chemistry or coagulation testing.
5 min Produces separation equivalent to 3 min setting.
Centrifugal forces are decreased to accommodate.
larger glass tubes which require lower g-forces.
7200 rpm 4440 xg
7200 rpm 4440 xg
5600 rpm 2685 xg
Tube Guide by Settings
Tube
Size
5 mL SV08 13x75 mm Plastic Plastic/Gel Glass/Plastic/Gel
7 mL SV06 16x75 mm Plastic Plastic/Gel Glass/Plastic/Gel
7 mL SV09 13x100 mm Plastic Plastic/Gel Glass/Plastic/Gel
10 mL SV07 16x100 mm Plastic Plastic/Gel Glass/Plastic/Gel
Insert Tube
Dimension
2 minute 3 minute 5 minute
6
Unloading
1. Upon completion of the cycle, the rotor decelerates to a complete stop in 30 seconds or
less, a signal beep will sound, and the cover latch automatically releases.
2. Lift the cover.
3. Carefully remove the tubes using care not to re-suspend the sample.
7
Overview
Cleaning
Section 4
Maintenance
Iris Sample Processing recommends that instrument operators perform periodic inspections and
preventative maintenance on all instruments. Contact customer service department or your
distributor if the instrument is not functioning properly.
CAUTION - Unplug the Express 3 from the wall outlet before cleaning or performing
maintenance.
WARNING – Do not expose the Express 3 or its rotor to strong or concentrated acids,
bases or oxidizing agents, aromatic or halogenated hydrocarbons, esters or ketones.
The Express 3 is supplied with a disposable bowl liner to simplify routine cleaning. Additional bowl
liners are available (Product No. DL02). The liner should be replaced as needed. Clean the outside
surfaces and switch overlay panel with a water-dampened cloth and mild detergent. Clean the inner
surface or bowl with a mild detergent, and if necessary, a disinfectant, wiping surfaces with a
dampened cloth using 70% alcohol or 10% bleach solution.
CAUTION - Do not spray cleaning solutions directly onto the centrifuge bowl or
housing; overspray could reach the motor bearings or internal circuitry. Subsequent
damage may not be covered by warranty.
General Cleaning
1. Open centrifuge cover.
2. Unplug centrifuge from the electrical outlet.
3. Remove all tubes and inserts. Remove the rotor (see Removing and Installing the Rotor).
4. Remove the black gasket surrounding the chamber by pulling up one end of the gasket.
5. Remove the bowl liner and dispose. Install a new bowl liner.
6. Replace the black gasket around the chamber opening.
7. Re-install rotor (see Removing and Installing the Rotor).
8. Plug into electrical outlet.
Rotor Disinfecting
1. Open centrifuge cover.
2. Unplug from the electrical outlet.
3. Remove all tubes and inserts. Remove the rotor (see Removing and Installing the Rotor).
4. The rotor and inserts may be soaked in detergent and warm water or a 10% bleach solution.
5. DO NOT attempt to clean an insert that has a broken tube. Dispose of insert immediately.
6. Dry the rotor and inserts with a clean absorbent paper towel or allow to air dry.
7. Re-install the rotor (see Removing and Installing the Rotor).
8. Plug into electrical outlet.
Checking the Rotor Speed
The rated speeds (+5%) can be checked with a stroboscope or photoelectric tachometer. If the
Express 3 fails to achieve an operating speed of 7200 rpm
5600 rpm
service department.
+5% in the 5 min setting, contact your distributor or the Iris Sample Processing customer
+5% in the 2 min and 3 min settings or
8
Troubleshooting
No power light
↓
Is the line cord completely plugged
into the wall?
↓
Yes
↓
Is the wall outlet working properly?
↓
Yes
↓
Is the fuse blown?
↓
No
↓
Contact Iris Sample Processing or
an Authorized Repair Facility.
Centrifuge will not spin, or shuts
off prematurely
↓
Is the cover closed and latched?
↓
Yes
↓
Is the error light flashing?
↓
No
↓
Contact Iris Sample Processing or
an Authorized Repair Facility.
→ No→
→ No→
→ Yes→
→ No →
→ Yes →
Plug line cord into the wall.
Try another wall outlet.
Replace fuse.
Completely close the cover.
Identify source of error from flashing
indicator lights, correct the error condition,
and push the Stop/Open button to clear the
error.
Centrifuge will not open at end
of cycle
↓
Is the bowl gasket completely
seated and sealing the bowl?
↓
YES
↓
Contact Iris Sample Processing or
an Authorized Repair Facility.
→ NO→
Press the bowl gasket all the way down into
the housing slot.
9
Service
Refer all service to Iris Sample Processing or an Authorized Repair Facility. 1-800-782-8774
Instrument fuse(s) are located on the rear panel. Remove the line cord to access the fuse holder.
Replace with fuses of the same type and rating.
3.0A 250V F 5 x 20 mm
Be sure to complete the Warranty Registration Card as directed or go to
http://www.proiris.com/ to obtain a copy of the warranty card.
Decontamination before returning for service:
Any instrument or accessory that has been exposed to blood or other biological materials must be
cleaned prior to shipment to the manufacturer or distributor for service. This decontamination is
required by Federal Law (Title 48 and 49 of the Federal Regulations) and in accordance with the
Environmental Protection Agency’s Regulations for Biohazard Waste Management. Iris Sample
Processing personnel cannot perform this decontamination.
10
Specifications
Product Number
Section 5
Appendix
SSX3
Model Number
Spin Parameters
Acceleration Time
Deceleration Time
Electrical
Dimensions
Environmental
M502-22
2 min: 120 seconds @ 7200 rpm / 4440 xg
3 min: 180 seconds @ 7200 rpm / 4440 xg
5 min: 300 seconds @ 5600 rpm / 2685 xg
≤ 25 seconds
≤ 30 seconds
100 – 240 VAC, 50/60Hz, 3.0 Amps
Height: 7”
Width: 11”
Depth: 10”
Weight: 8¾ lbs.
For indoor use only
Ambient temperature 2ºC to 40ºC
Maximum 80% RH between 2ºC and 30ºC,
decreasing to 50% RH at 40ºC
Maximum altitude 2000 m
Specifications are subject to change without notice.
WARNING – Do not operate or store the Express 3 or its rotor below the 2
minimum operating temperature. At extreme low temperature (below -18
material is subject to cracking under impact stress (such as dropping onto a hard
surface).
Main supply voltage fluctuations should not exceed ±10%
Transient over-voltages according to installation category II
Pollution degree 2
o
C
o
C) the rotor
11
References
A Division of IRIS International, Inc.
Westwood, MA 02090 USA
Phone: 800-782-8774
or 781-551-0100
Fax: 781-551-0036
www.proiris.com
mdi Europa GmbH
Wittekamp 30
D-30163 Hanover Germany
+49-511-39089530
Made in USA
1. CLSI. “Protection of Laboratory Workers from Occupationally Acquired Infections;
Approved Guideline-Second Edition.” CLSI document M29-A2 [ISBN 1-56238-453-8].
CLSI, 940 West Valley Rd, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2001.
2. CDC. Recommendations for Prevention of HIV Transmission in Health Care Settings.
MMWR (Suppl. No. 2S):2S-18S, 1987.
3. CDC. Updated: US Public Health Service Guidelines for the Management of Occupational
Exposures to HBV, HCV and HIV and Recommendations for Post Exposure Prophylaxis.
Appendix A and B. MMWR 50 (RR-11): 1-42, June 29, 2001.
55-005187-001 Rev. D
12
Iris Sample Processing Warranty
Iris Sample Processing, a Division of IRIS International, Inc. warrants that the instruments shall be free from
defects in material and/or workmanship, under normal use and service, for the period expiring twenty-four (24)
months from the date of installation, provided the purchaser has completed and forwarded to Iris Sample
Processing the Warranty Registration Card. Iris Sample Processing will, at its discretion, repair or replace any unit
covered under this warranty returned to Iris Sample Processing with shipping costs prepaid. Repaired or replaced
instruments supplied under this warranty carry only the remaining portion of the original warranty and repairs shall
not interrupt or prolong this warranty. For warranty terms and conditions outside the United States, contact your
Authorized Iris Sample Processing Distributor.
No warranty extended by Iris Sample Processing shall apply to any instrument that has been damaged due to
misuse, negligence, accident, or damage resulting from unauthorized repairs, alterations, or improper installation.
Iris Sample Processing makes no warranty other than the one set forth herein. This warranty is given expressly in
lieu of all other warranties, expressed or implied. The purchaser agrees that there is no warranty of
merchantability or of fitness for any intended purpose and that there are no other remedies or warranties,
expressed or implied, which extend beyond the description on the face of the agreement. No agent or employee of
Iris Sample Processing is authorized to extend any other warranty or assume for Iris Sample Processing any
liability except as set forth above. This warranty is only applicable to the original purchaser.
Limitation of Liability
Iris Sample Processing shall not be liable for any loss of use, revenue or anticipated profits, or for any
consequential or incidental damages resulting from the sale or use of the products. The purchaser shall be
deemed liable for any and all claims, losses, or damages incurred by the use or misuse of the Iris Sample
Processing instrument by the purchaser, its employees or others, following receipt of the instrument or other items.
13
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