Invitrogen PowerEase 500, PowerEase 500 EI8600, PowerEase 500 EI8700, PowerEase 500 EI8675 Instructions For Use Manual

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SM
View
Instra
PowerEase® 500 Power Supply
Version D
032202
IM-8634
PowerEase® 500 Power Supply
A programmable power supply for electrophoresis
Catalog nos. EI8600, EI8700, EI8675
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www.invitrogen.com
tech_service@invitrogen.com
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Warning
Federal Communications Commission Advisory
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at their expense.
Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
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Table of Contents
Warning.................................................................................................................................................................iii
Table of Contents...................................................................................................................................................v
Kit Contents ..........................................................................................................................................................vi
Description...........................................................................................................................................................vii
Safety Information................................................................................................................................................xi
Consignes De Securite ........................................................................................................................................xii
Sicherheits Anweisungen..................................................................................................................................xiii
Accessory Products............................................................................................................................................xiv
Introduction....................................................................................................................1
Overview................................................................................................................................................................ 1
Methods..........................................................................................................................3
Getting Started....................................................................................................................................................... 3
Operational Modes................................................................................................................................................ 4
Using PowerEase® 500.......................................................................................................................................... 5
Printing ................................................................................................................................................................. 12
Custom Methods ................................................................................................................................................. 13
Troubleshooting ..................................................................................................................................................16
Appendix ......................................................................................................................17
Warranty............................................................................................................................................................... 17
Run Conditions for Gels..................................................................................................................................... 18
Repair and Maintenance of PowerEase® 500................................................................................................... 19
Theory of Operation............................................................................................................................................ 20
Technical Service................................................................................................................................................. 21
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Kit Contents
Types of Kits
Kit Components
This manual is supplied with the following kits:
Kit Catalog no.
PowerEase® 500 Power Supply (100/120 Vac 50/60 Hz) EI8600
PowerEase® 500 Power Supply (220/240 Vac 50/60 Hz) EI8700
PowerEase® 500 Pre-Cast Gel System
includes PowerEase® 500, Temperature Monitoring Probe, XCell
SureLock™ Mini-Cell and XCell II™ Blot Module
EI8675
PowerEase® 500 Power Supply contains:
PowerEase
®
500 Power Supply 1 each
Instruction Manual 1 each
Extra Fuses 2 each
Power Cord (U.S., Canada, Europe, Taiwan and Japan) 1 each
Warranty Card 1 each
Temperature Probe (Optional) 1 each
See next page for specifications and detailed description of PowerEase
®
500
Power Supply.
To ensure safe, reliable operation, always operate the PowerEase® 500 Power Supply in accordance with the manufacturer’s instructions. Always wear protective gloves and safety glasses when working in a laboratory environment. See safety information on pages xi-xiii.
Warranty information is provided on page 17.
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Description
Specifications
Input Power
100/120 VAC 90-132V, 50/60 Hz, 100 watts at maximum load
Fuse One 1A/250V/AGC, fast blow fuse
220/240 VAC 198-264VAC, 50/60 Hz, 260 mA at maximum load
Fuses Two F1A/250V/5x20 mm fast blow fuses
Output Range 1-500 V (minimum step size 1 V)
1-500 mA (minimum step size 1 mA)
0.1-50 W (minimum step size 0.1 W)
Automatic crossover on reaching set limits.
Accuracy Voltage: ± 2% or ± 2 volts
Current: ± 2% or ± 2 mA
Wattage ± 2% or ± 0.2 watt
Drift < 1% in 8 hours after 30 minute warm-up with constant supply voltage
Circuit Protection Open Circuit; Short Circuit; Thermal Protection Over Voltage, Current, Wattage
Safety Load Detection
Thermal Shutoff (with optional temperature probe)
Isolated Floating Ground
Memory Control Non-volatile, up to 24 hours
Custom Methods Stored to RAM
Computer Interface RS232C
Display LCD, view area 114 x 64 mm
240 x 128 dots
Temperature Ambient 4-30°C
Dimensions 16.5 cm (w) x 18 cm (h) x 20.5 cm (d)
Weight 2.9 kg (6.4 lbs)
Environmental Conditions 100% RH, 75 KPa-106 KPa
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Continued on next page
Description, Continued
Front View of PowerEase
®
500
1
8
POWER
5
+
2
S
3
R
A
T
T
S
O
P
T
1. LCD Screen
2. Power Output Jacks, Positive (Red)
3. Power Output Jacks, Negative (Black)
4. Temperature Probe Jack
5. Functional Keys (Power, Start, Stop)
6. Directional Keys (Up, Down, Left, Right)
7. High Voltage LED
8. Power LED
P
SELECT
o
w
r
e
HIGH VOLTAGE
5
e
s
a
E
TEMP
7
0
0
6
4
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Continued on next page
Description, Continued
Rear View of PowerEase
®
500
Fan
Printer Port
PRINTER
Power Entry Module with On/Off main power switch
RS232
RS232C Communi­cation Port
Continued on next page
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Description, Continued
Key Pad
Power button secondary On/Off switch for the high voltage components, fan, and LCD
SELECT
screen.
Start button used to start a selected method program.
Stop button used to end the selected method and to backup to a previous screen.
Select button used to select a menu choice and to move forward to the next applicable screen.
Left directional button for moving left around the menu.
Right directional button for moving right around the menu.
Up directional button for moving up around the menu and to adjust numerical and
character values (higher).
Down direction button for moving down around the menu and to adjust numerical and character values (lower).
x
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Safety Information
Avoiding Electrical Shock
Avoiding Damage to the Instrument
The PowerEase® 500 Power Supply produces high voltage outputs which are electrically isolated from earth ground to reduce the risk of electrical shock to the user. Observe the following guidelines to ensure safe operation of the unit.
The PowerEase electrophoresis cells with shielded banana plugs thus minimizing potential shock hazard to the user. Invitrogen does not recommend use of other unshielded banana plugs.
To avoid electrical shock:
1. NEVER connect or disconnect wire leads from the power jacks when the amber high voltage indicator light is on.
2. WAIT at least 5 seconds after stopping a run before handling output leads or connected apparatus.
3. ALWAYS make sure hands and work area is clean and dry before making any connections or operating the power supply.
4. ONLY connect the power supply to a properly grounded AC outlet.
1. For proper ventilation, leave at least 10 cm of space behind the instrument, and at least 5 cm of space on each side.
2. Do not operate the power supply in high humidity environments (> 95%), or where condensation can occur.
3. To avoid condensation after operating the power supply in a cold room, wrap the unit in a plastic bag and allow at least 2 hours for the unit to equilibrate to room temperature before removing the bag and operating the unit.
®
500 Power Supply has been designed for use with
Symbols
The symbols used on the PowerEase® 500 Power Supply are explained below.
Used on the PowerEase® 500 Power Supply to indicate an area where a potential shock hazard may exist.
Used on the PowerEase® 500 Power Supply to indicate a warning. Consult the manual to avoid possible personal injury or instrument damage.
Used on the PowerEase
®
500 keypad to indicate the secondary Power button.
Used on the PowerEase
Used on the PowerEase
®
500 keypad to indicate the Start method button.
®
500 keypad to indicate the Stop method button.
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