Invitrogen Evos XL User Manual

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EVOS

™

XL Imaging System

For Transmitted Light Applications

Catalog Number AME3300

Publication Number MAN0007987

Revision A.0

For Research Use Only. Not for use in diagnostic procedures.

Inform ation in thi s docum ent is subject to change without noti ce.

Revision

Date

Description

A.0

09 October 2017

Update branding, legal/regulatory language, reorganize content.

1.0

8 April 2013

Basi s for t his r evi sion.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIA TE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Revision history: MAN0007987

Impo rtant Lic ensing Info rmat ion: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses.

Manufac turer: Life Technologies Corporation | 22025 20th Ave SE St #100 | Bothell, WA 98021

Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is a registered trademark of Kimberly-Clark Corporation.

Contents

About this guide......................................................................................................................... 3

1. Product information ........................................................................................................... 4

Product description .......................................................................................................................................................... 4

Standard items included ................................................................................................................................................ 4

Instrument exterior components and mechanical controls ................................................................................ 5

Onscreen cont rols ............................................................................................................................................................. 6

2. Installation ........................................................................................................................... 7

Operating environment .................................................................................................................................................. 7

Transport the EVOS™ XL Imaging System to the installation site ................................................................... 7

Unlock mechanical stage ................................................................................................................................................ 8

Connect p ower supply.................................................................................................................................................... 8

USB and D VI outpu t ports ............................................................................................................................................ 8

Condenser shield .............................................................................................................................................................. 9

Install the E VOS™ XL Imaging System in a c ell culture hood ..........................................................................10

3. Basic operation.................................................................................................................. 11

4. Advanced operation .......................................................................................................... 14

Manage users ...................................................................................................................................................................14

Save images and manage fil es ....................................................................................................................................16

Use the QuickSa ve option to save multiple i mages ............................................................................................18

Record time lapse images ............................................................................................................................................20

Count cells.........................................................................................................................................................................23

Review images .................................................................................................................................................................24

Use digital zoom .............................................................................................................................................................25

Connect the E VOS™ XL Imaging System to a network ......................................................................................28

Update the software.......................................................................................................................................................31

5. Care and maintenance ..................................................................................................... 33

General care ......................................................................................................................................................................33

Objec tive lens care ..........................................................................................................................................................33

Stage care ...........................................................................................................................................................................33

Decontamination p rocedures .....................................................................................................................................34

EVOS™ XL Imaging System User Guide

Appendix A: Troubleshooting ................................................................................................ 35

Image quality issues.......................................................................................................................................................35

Software inte rface issues ..............................................................................................................................................36

Mechanical issues ...........................................................................................................................................................36

Appendix B: System specifications ...................................................................................... 37

Appendix C: Controls glossary.............................................................................................. 38

On screen c ontrols ..........................................................................................................................................................38

Mechanical controls .......................................................................................................................................................42

Appendix D: Safety .................................................................................................................. 44

Safety conventions used in this document.............................................................................................................45

Symbols on instruments ...............................................................................................................................................46

Safety labels on instruments .......................................................................................................................................48

General instrument safety............................................................................................................................................49

Chemical safe ty ...............................................................................................................................................................50

Chemical waste safety...................................................................................................................................................51

Electrical safety................................................................................................................................................................52

Physical hazard safety...................................................................................................................................................53

Biological hazard safety................................................................................................................................................53

Safety and electromagnetic compatibility (EMC) standards............................................................................54

Documentation and support .................................................................................................. 55

Obtaining support ..........................................................................................................................................................55

EVOS™ XL Imaging System User Guide

Audience

This user guide is for laboratory staff operating, maintaining, and analyzing data

User attention words

Two user attention words appear in Thermo Fisher Scientific user documentation.

Safety alert words

Four safety alert words appear in Thermo Fisher Scientific user documentation at

level of observation or action, as defined below:

Except for IMPORTANT! safety alerts, each safety alert word in a Thermo Fisher

SDSs

The Safety Data Sheets (SDSs) for any chemicals supplied by Thermo Fisher

SDSs, see “Safety Data Sheets (SDS)”, page 55.

About this guide

using the EVOS™ XL Imaging System.

Each word implies a particular level of observation or action as described below.

points in the document where you need to be aware of relevant hazards. Each alert word—IMPOR TANT, CAUTION, WARNING, DANGER —implies a particular

Note: Provides information that may be of interest or help but is not critical to the use of the product.

IMPORTANT! Provides information that is necessary for proper instrument operation, accurate installation, or safe use of a chemical.

IMPORTANT! – Provides information that is necessary for proper instrument operation, accurate installation, or safe use of a chemical.

CAUTION! – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

WARNING! – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury.

DANGER ! – Indicates an imminently hazardous situation that, if not avoided, will result in death or serious injury. This signal word is to be limited to the most extreme situations.

Scientific document appears with an open triangle figure that contains a hazard symbol. These hazard symbols are identical to the hazard symbols that are affixed to Thermo Fisher Scientific instruments (see “

Scientific are available to you free 24 hours a day. For instructions on obtaining

IMPORTANT! For the SDSs of chemicals not distributed by Thermo Fisher Scientific contact the chemical manufacturer.

EVOS™ XL Imaging System User Guide

Safety symbols ”, page 47).

1. Product information

EVOS™ XL Imaging

The Invitrogen™ EVOS™ XL Imaging System (Cat. No. AME3300) is a fully

assist cell counting, and image review.

Product use

For Research Use Only. Not for use in diagnostic procedures.

Before setting up the EVOS™ XL Imaging System, unpack the unit and accessories

• EVOS™ XL Imaging System, per order

Condenser slider: Diffuser

Product description

System

automated, digital, inverted imaging system. The system is designed for a broad range of transmitted light applications including time-lapse imaging, manual-

Standard items included

and verify all parts are present. Contact your distributor if anything is missing. Damage claims must be filed with the carrier; the warranty does not cover intransit damage.

Note: If you do not have your distributor information, contact Technical Support (page 55).

• Power adapter and power cord

• Dust cover

• USB flash drive (includes User Guide and Quick Start Guide)

• Condenser shield, removable

• Accessories box

• USB mouse

• Stage lock pin (in place on stage)

•

IMPORTANT! Wiping the computer supplied with the EVOS System (i.e., erasing the hard drive to remove all programs, files, and the operating system) voids the product warranty. Do not install third party software or update the operating system.

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XL Imaging

EVOS™ XL Imaging System User Guide

Instrument exterior components and mechanical controls

Power switch

Power input jack

USB and DVI ports

Coarse stage positioning knobs

Stage X-axis knob

Objective selection wheel

Focus knobs

Stage Y-axis knob

Phase annuli selector

Condenser slider slot

EVOS™ XL Imaging System User Guide

Onscreen controls

Light ON/OFF button

Illumination slider

Capture image button

Color adjustment button

Note: See “Appendix C: Controls glossary” on page 38 for more details about onscreen and mechanical controls.

Scale bar and toolbar options

Settings control button

Save image button

QuickSave option

Selected objective

EVOS™ XL Imaging System User Guide

• Place the EVOS™ XL Imaging System on a level surface away from vibrations

• Relative humidity range: 30–90%.

XL Imaging System to be subjected to

2. Installation

Operating environment

from other pieces of equipment.

• Allow at least 5 cm (2 in) free space at the back of the instrument to allow for proper ventilation and prevent overheating of electronic components.

• Set up the EVOS™ XL Imaging System away from direct light sources, such as windows. Ambient room lighting can enter the imaging path and affect the image.

• Operating temperature range: 4°–32°C (40°–90°F).

IMPORTANT! EVOS sterilization. UV degrades many materials, including plastic. Damage from

UV exposure is not covered under the manufacturer’s warranty.

™

XL Imaging System should not be subjected to UV

Transport the EVOS™ XL Imaging System to the installation site

1. IMPORTANT! ALWAYS lock stage with the stage lock pin before moving the instrument.

2. Lift the instrument by grasping it firmly with both hands under the support arm , balancing the weight as shown in the image below.

3. To transport the EVOS original packaging materials if possible. Always be sure the instrument is properly cushioned and braced to prevent damage.

IMPORTANT! Never allow the EVOS™ sudden impact or excessive vibration. Handle the instrument with care to

prevent damage.

™

XL Imaging System to a different facility, use the

EVOS™ XL Imaging System User Guide

Unlock mechanical stage

Remove stage lock Connect power

USB ports

Plug the mouse and the USB flash drive into

DVI port

Before using the mechanical stage for the

first time, remove the stage lock pin from the back right-hand corner of the stage plate.

Pull firmly to remove this pin.

Note: Always secure the mechanical stage with the stage lock pin before moving the instrument. Store the stage lock pin in your accessories box for

future use.

Connect power supply

1. Turn the power switch to the “ ” (OFF)

adapter

position before connecting the power adapter.

2. Connect the power adapter to the power jack on the right side of the instrument base, attach the cord to the adapter, and plug the cord into an outlet.

USB and DVI output ports

the USB ports located on the bottom right of the support arm. You may also plug in a USB keyboard (not included) for text input.

A DVI port allows output to a projector or other display (DVI cable not included). This port produces digital output only; EVOS Imaging System is compatible with either a DVI-D or DVI-I display.

IMPORTANT! Always use the correct power supply. The power adapter specifications appear on the serial number label (front of LCD hinge) and in “Appendix B: Specifications”, page 37. Damage due to an incompatible

power adapter is not covered by warranty.

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EVOS™ XL Imaging System User Guide

This sh ield can help reduce the effects th at overhead lighting might have on your

Condenser shield

image.

EVOS™ XL Imaging System User Guide

EVOS™ XL Imaging System’s small footprint and simple power connection allow it to be installed quickly to be conveniently used in a cell culture hood.

Dimensions

The EVOS™ XL Imaging System will fit in cell culture hoods that are at least

Install the

Install the EVOS™ XL Imaging System in a cell culture hood

20½ inches (520 mm) deep. If your cell culture hood is smaller, it may be possible to turn the instrument at a slight angle to fit.

instrument

1. Secure the stage with the stage lock pin, switch the EVOS™ XL Imaging System

2. Tilt the LCD screen back until it is parallel with the tabletop.

3. Lift the instrument by grasping it firmly with both hands under the support

4. Gently place the instrument on a lab cart and transport it to the cell culture

5. Lift the instrument as before and move it into the hood.

6. Tilt the LCD monitor upright.

7. Remove the stage lock pin, connect the power cord, mouse and, if desired,

Height, transport

Height, display

Note: See the illustrations on page 7 for details about moving the EVOS

XL Imaging System.

off, and disconnect the power cord, mouse and, if connected, keyboard.

arm just behind the condenser.

hood.

Note: Verify that the hood sash is raised enough for the instrument to slide underneath (approximately 14 inches or higher).

keyboard, and switch the instrument on.

Depth

Width

Weight

English Metric

18.5 in 47.0 cm

14.0 in 35.5 cm

12.75 in 32.4 cm

22.75 in 57.8 cm

31.4 lbs 14.2 kg

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EVOS™ XL Imaging System User Guide

The EVOS™ XL Imaging System uses both mechanical and software controls for

1. Turn on the instrument using the power switch

3. Basic operation

operation. Mechanical controls include the stage X-Y axis knobs, focus knobs, and the objective selection wheel. Software controls are located in the control bars at the bottom of the screen. The login button displays the current user ID.

See “Instrument exterior components and mechanical controls” (page 5), as needed. See also “Appendix C: Controls glossary” (page 38) for more details.

on the right side of the instrument base.

EVOS™ XL Imaging System User Guide

2. Plug a USB flash drive into one of the USB ports on the right side of the instrument arm.

3. Place the sample on the stage, using a vessel holder if needed.

4. Set the magnification using the objective selection wheel located in the front of the instrument.

7. Turn on illumination using the Light ON/OFF toggle in the left control bar.

5. Turn the phase annuli selector to the position that corresponds to the selected objective and contrast method.

6. Insert the appropriate condenser slide into the slot on the condenser assembly.

Note: A Diffuser slider can be used for low magnification (2× or 4×) brightfield applications to improve flat field illumination.

8. Focus the sample using the focus knobs .

9. Adjust the illumination intensity if necessary, using the Illumination slider on the left control bar or the mouse scroll wheel.

10. Click the Capture button , in the left control bar to acquire the image.

11. Click the Save button , located in the right control bar to save the color image. The “Save File dialog” opens.

Note: Overexposed pixels appear red. To get the maximum level of brightness without any overexposed areas, dim the illumination until the red highlights disappear.

EVOS™ XL Imaging System User Guide

12. To enter the file name, click in the Save File Name field .

The virtual keyboard opens.

13. Enter the file name, then click the Accept button .

14. Choose the file type and click the Save button .

Note: See “Save images and manage files” (page 16) for more information.

EVOS™ XL Imaging System User Guide

4. Advanced operation

EVOS™ XL Imaging System keeps settin gs in memory for each us er ID, s o that

1. Click the Login button at the bottom left of the screen (this is the

Add or remove a

1. Click the Login button.

Manage users

multiple users can work with the same EVOS™ instrument with out ha vin g to reset their preferen ces.

To use this feature, set up a user profile for eac h regula r user. Y ou can also assign user IDs for ex periments in progress .

Note: User profiles are not password protected. All users should verify they are logged in correctly to avoid changing other users’ settings.

existing profile

user profile

Invitrogen logo with the current user profile).

2. Login dialog opens. Select the desired user profile, then click OK.

Note: No password is necessary to log in.

2. To copy an existing profile, highlight the profile from the user list, select the Copy from ‘name’ option, then click Add. The virtual keyboard opens for you to assign name the new profile.

3. To create a new user profile without copying any settings, deselect Copy from option, click Add, then enter a user name.

4. After adding a new user profile, click OK to log in under that name and adjust settings as desired. When you switch off, the EVOS sa ves your settings to memory.

5. To remove a user profile, highlight it and click Remove. A confirmation dialog opens. Deleting the user profile will remove all its associated settings from memory.

6. To rename a user profile, click Rename and enter the new name.

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XL Imaging System

EVOS™ XL Imaging System User Guide

Change the default

The default user login is Guest; to set the default login as the last active user, go to

the Basic tab of the Settings dialog and uncheck the Default to Guest on startup option.

Note: For multiple users, we recommend leaving the “Default to Guest on startup” option checked.

EVOS™ XL Imaging System User Guide

Save images and manage files

Save images

1. Click Save to open the “Save file” dialog.

In the “Save Folder list” and the “Saved files list”, selected items appear blue.

Note: If a USB keyboard is installed, the virtual keyboard does not appear. Pressing the Enter key on a physical keyboard is equivalent to clicking the Save button in the Save file dialog.

2. If there is no USB flash drive or network connection in place, an information message appears in red. Click Cancel to clear this message.

EVOS™ XL Imaging System User Guide

3. Click in the Save File Name field to enter a file name. If you are using the

4. Click on the name of a folder in the “Save Folder list” to select the destination

5. To create a new folder, first click the name of the parent folder, and then click button.

virtual keyboard, click Accept after entering the text. The “Save As confirmation dialog” opens.

To overwrite a file, simply select the name of the file from the saved files list instead of clicking on the Save File Name field.

Note: It is not possible to recover an overwritten file.

for the new image.

the New Folder bu tton. This a ctivates the virtual keyboard (if it is needed) so you can enter a folder name and date.

Note: Click the Date button anywhere within a field to automatically insert the current date (MM-DD-YYYY) wherever the cursor is in that field.

6. Select a file format (.tif, .png, .jpg or .bmp) from the File Type dropdown.

7. Optional: Click in the Comment field to enter a comment and date.

8. Click Save.

9. To delete a file or folder, highligh t the item on the list, then cli ck the De lete A confirmation dialog opens. It is not pos sible to recover a deleted fil e.

10. To rename a file or folder, highlight the item on the list and click Rename. The virtual keyboard opens. You can click Clear to reset to a blank field.

EVOS™ XL Imaging System User Guide

QuickSave allows you to save multiple images under a single base file name.

1. Click Settings to open the “Settings dialog”, then select the QuickSave tab.

Use the QuickSave option to save multiple images

When you specify the settings and select the QuickSave option, the EVOS™ XL Imaging System saves each image with a single click of the Save button.

2. Click in the Base Filename field, then enter a name that describes the capture session. The blue “Next” file name reflects the information entered.

3. Click in the Count field to enter the starting number, if you do not want to start at 1. The blue “Next” file name reflects the information entered.

4. Select a file format (.tif or .png) from the File Type dropdown.

5. Click Browse to select a destination folder for the Quick Save files. In the Browse dialog that opens, highlight the desired folder, then click OK.

6. To create a new folder, first click the name of the desired parent folder, then click New Folde r. Enter a fold er na me and date, if desired. After crea ting the new folder, click OK to close the Browse dialog.

EVOS™ XL Imaging System User Guide

specified in the QuickSave settings.

Note: Click the Date button anywhere within a field to automatically insert the current date (MM-DD-YYYY) wherever the cursor is in that field.

7. Click OK to accept QuickSave settings.

8. In the right control bar, select the QuickSave option.

9. After acquiring an image with Capture, click Save. The image will be saved as

EVOS™ XL Imaging System User Guide

Record time lapse images

Time lapse tool

With the Time laps e tool, you can s et up your cells a nd program the EVOS™ XL Imaging System to record time la pse images.

Start a time lapse

1. Once the s a mple is focused and

3. In Time Lapse Settings, click the

session

ready, tighten the stage brakes to prevent the stage from drifting during the session.

2. Open the Toolbar and expand the

Time lapse tool.

Interva l field, then enter a value.

Choose a unit of measure for the capture interval from the Interva l Unit dropdown.

4. Click the Duration field and enter a

value. Choose a unit of measure for

the duration of the capture session from the Duration Unit dropdown.

Note: The Images field shows the total number of images for the session.

5. To save the session in a folder other than the default location, click Browse to select the destination.

6. In the Browse dialog, highlight the desired folder, then click OK.

7. To save the session in a folder other than the default location, click Browse to select the destination.

8. In the Browse dialog, highlight the desired folder, then click OK.

9. Under File, click the Base Filename field to enter a name, then choose a file type (.png or .tif) from the File Type dropdown.

10. To create a video (.avi) file, select Write Video File.

EVOS™ XL Imaging System User Guide

11. Click Start to begin the time lapse session. The system displays the Time

Pause and restart a

1. In the Time Lapse Progress dialog, click Pause to suspend the time lapse

Lapse Progress dialog as long as the session is active.

Note: The Review slider lets you review the images already captured during the current session. Click Play to view all images in sequence.

time lapse session

session. The progress dialog dims, and a Resume button replaces the Pause button.

Alternatively, to pause and adjust the settings, click Find & Focus.

2. Click Start to resume the time lapse capture sequence, or uncheck the Resume option and start a new time lapse session.

EVOS™ XL Imaging System User Guide

Abort a time lapse

1. In the time lapse progress dialog, click Abort. A new dialog opens and gives

session

you the option to delete or keep the files already saved.

2. To resume the session, click Cancel to return to the time lapse progress dialog.

EVOS™ XL Imaging System User Guide

Count tool

The Count tool s treamlines cel l counting by marki ng items w ith up to six labels

Count cells

1. Acquire an image. See “Basic

image saved with the tags only.

Count cells

onscreen. As you tag items, the system keeps a running tally of counts with percenta ges for eac h label a ssigned. You ca n document your res ults by saving the tagged image w ith the Coun t tool dis playing the total s.

operation” (page 11) for detailed instructions.

2. Open the Toolbar, then expand the Count tool.

3. Click in a Label field to enter a name for that label. You can use up to six labels.

4. Under Settings, choose the desired grid size using the Grid size

dropdown or leave the Show Grid option inactive.

5. Select a Label, then left-click at each point onscreen to tag the items for that category. Switch labels as desired; the EVOS System will tag for the selected label.

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XL Imaging

6. To move a tag, select and drag it. Left-click anywhere else onscreen to deselect.

7. To delete a ta g, right-click the ta g. Alternatively, choose th e Delete mode, then left-click the ta g.

8. To delete all tags for all labels, c lick the Clea r All button.

9. To save an image showing the labels, counts, and percentages as shown in the Count tool, select the Save Image with Toolbar option, then click Save on the right toolbar. Deselecting this option produces an

Note: To use Digital Zoom while counting cells, first suspend the Count tool. Either select the Hide Tags setting, click the triangle to minimize the Count tool, or click the Toolbar option to minimize the whole Toolbar. When the Count tool is suspended, the left mouse button will behave according to the rules described in “Use digital zoom” (page 25). After zooming, reactivate the Count tool by deselecting Hide Tags or reopening the tool.

EVOS™ XL Imaging System User Guide

Image review tool

The Image review tool allows you to review still images or play video files from the USB drive or network connection. You can a lso use th is tool to ren ame or delete s aved files.

Review images

1. Open the Toolbar, then expand the

level USB

7. To rename a file, select it and click Rename. The Virtual Keyboard opens.

Review images

Image review tool.

2. The preview list displa ys thumbnail images for all viewable files in the selected directory (the topdirectory is selected by default). If there are no viewable files in the directory, the preview area remains empty.

Note: The File Type dropdown filters files by type. By default, it is set to display all files with .png, .tif, .jpg, .bmp, or .avi extensions.

3. If the image or video file you wish to review is not in the directory displayed, click Browse to find and open the desired directory.

4. Use the scroll bar as needed to search the preview list for the desired image file. Click the image to select it. The selected file name appears blue.

5. Click View File to display the image in the image review window. This button toggles between View File and Hide File. Hide the file to close the image review window. You can also double-click the thumbnail image to toggle between displaying and hiding the file.

6. To zoom the image in the review window, double-click the area of interest; right-click to restore normal magnification. See “Use digital zoom” (page 25) for more detailed instructions.

Enter the new file name, then click Accept.

8. To delete a file, select it and click Delete. A confirmation dialog opens. It is not possible to recover a deleted file.

9. Click Slide Show to display each image thumbnail within the image review window continuously.

EVOS™ XL Imaging System User Guide

The EVOS™ XL Imaging System ca n zoom in the i mage ons creen, a llowing you a

dis play appea rs over the selected objective indicator.

Zoom in and

2. In the enlarged ima ge, double-click on a ny point in th e middle a rea of the screen

3. Right-click anywhere on the image to restore the view to unzoomed magnification.

Use digital zoom

clos er look. Double-click li ve or ca ptured i mages to z oom in to them. In the images below, the numbered arrows indicate the click points. Note that the z oom factor

recenter live images

1. Double-click the area of interest in the image onscreen. EVOS™ imaging

Note: Live images can only zoom in to 2×. To zoom in to an image at higher levels, you must first capture it.

system displays a view zoomed in at 2×, centered on the point clicked.

Live image at 40× magnification

to re-center the image. The system places points from the outer edges of the screen as close to the center as possible. You can re-center repeatedly.

Live 40× image z oomed 2×, re-centered

EVOS™ XL Imaging System User Guide

Zoom in on

1. Double-click the area of interest in the image onscreen. The screen displays

2. In the enlarged image, double-click again on any point to double the

Cap tured 40× image zoom ed 4×

captured images

the zoomed in at 2× and centered on the clicked point.

Captured image at 40× magnification, with scal e bar

Cap tured 40× image zoom ed 2×

digital zoom level. The enlarged image centers around the point clicked.

EVOS™ XL Imaging System User Guide

3. Continue double-clicking to double the digital zoom value as desired. It is

4. Right-click to restore the view to unzoomed magnification.

possible to zoom in to the pixel level of the digital image.

Cap tured 40× image zoom ed 8x

Note: Capturing and saving a zoomed image results in a file showing the actual magnification, not the zoomed magnification. If the scale bar is active, it appears in the file.

EVOS™ XL Imaging System User Guide

You can log the EVOS™ XL Imaging System onto a Windows/SMB network via an

Items needed for

To set up the network connection, you need the following items (not included):

USB-to-Ethernet adapter

Log onto the

1. Verify the instrument is powered on and the network cable is plugged into the

3. Click the Settings button to open the Settings dialog, then select the Network

Connect the EVOS™ XL Imaging System to a network

Ethernet cable connection and save captured images directly to shared folders on the network.

Note: SMB is the only supported protocol. No other protocols (such as HTTPS, FTP, or WebDAV) are currently supported. If you are connecting to a Linux server, it will have to use Samba for EVOS to find it. Contact your network administrator for help if a physically connected EVOS

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XL Imaging System cannot find the SMB network.

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XL Imaging System

ethernet connection

network

• Ethernet cable

•

correct jack and connected via the adapter to the USB port.

2. Be sure you are logged in under your own EVOS

™

user ID. The current user ID is displayed above the Invitrogen logo in the bottom left corner of the screen. See “Manage users” (page 14) for more information.

tab.

™

Note: EVOS If there is a problem with the connection, the Network page will display

XL Imaging System will try to connect for about 30 seconds.

“No Items.”

Double-check the physical connections and click Refresh Network. During the refresh, a progress icon appears.

Unless there is an issue with the network, or you are using an incompatible adapter, refreshing the connection should resolve the problem within a few moments. Contact your network administrator for help if the problem persists.

If your configuration requires using a static IP address, select the Use Static IP Address option, enter your IP Address, Subnet Mask, Gateway Address, and DNS Server Address, then click Set Static IP.

EVOS™ XL Imaging System User Guide

4. The upper list box of the Network page will display the top level (available

5. Enter your network domain, user name, and password in the login fields,

domains) of the Windows/SMB network file tree. Click the triangle icon, or double-click the domain name, to expand a domain and display the available servers.

Note: If a domain, server, or shared file appears on the file tree without a triangle icon, and you are not able to expand or open it, your permission to access that item is restricted.

then select a server to view the top level of shared folders on that server. You can now navigate below the top-level shared folders on the Network page.

After the server accepts your login, EVOS™ XL Imaging System displays the list of available shared folders on the selected server.

EVOS™ XL Imaging System User Guide

6. Select a shared folder, then click Add to include it in the list of possible file

add multiple shared folders to the list, but you can only use a

7. If you need to remove a shared folder from the destinations list, select the

9. To verify your list of network destination folders, go to the QuickSave tab,

destinations.

Alternatively, type in the file path, then click Add. The folder should appear on the list box below the Add button. If it does not, contact your network administrator for help.

Note: You can single login on any one server or domain. We recommend that each EVOS™ user establish network connections under their own EVOS

Manage users” (page 14) for more information on EVOS™ user IDs.

“

Your EVOS instrument’s non-volatile memory). All your connections and login information are hidden from other EVOS

™

user ID remembers your network login (it is encrypted in the

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user profiles.

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user ID. See

folder name, then click Remove.

8. Click OK to close the Settings dialog.

then click Browse to display the QuickSave Browse dialog.

All your selected network destinations and any USB flash d rives currently plugged in appear in the Browse dialog.

These locations are also available in the Save dialog and through the Browse button in the Time lapse and Image review tools.

EVOS™ XL Imaging System User Guide

Periodically, Thermo Fisher Scientific adds functionality and other improvements

distributor information, contact Technical Support (page 55).

Download software

Software updates are available from the EVOS™ XL Imaging System product page

Download the update directly to the top level of a USB flash drive with at least

entation updates from

Install software

1. Plug the USB flash drive into the data port on the lower right side of the

3. Click Update in the Service tab. A verification progress bar appears.

Update the software

to the EVOS™ user interface. We recommend keeping your EVOS™ XL Imaging System up to date with the latest software. If you have any questions about software updates, contact your local EVOS

™

distributor. If you do not have your

update

at thermofisher.com/evosxl.

1. 30 MB available. Do not open or rename the file on your computer; EVOS™ XL Imaging System will verify and install it during the update procedure.

2. Download the current user guide for the EVOS™ XL Imaging System from our website. The updated user guide covers the new software functionality when features are added.

Alternatively, you can get the latest software and docum your local EVOS™ distributor or by contacting Technical Support (page 55).

EVOS™ support arm.

2. Click Settings to open the Settings dialog, then select the Service tab.

If a missing update notification appears, make sure that the USB flash drive with the update file is fully plugged in. Click OK, then click Update again.

EVOS™ XL Imaging System User Guide

4. After file verification, the update confirmation dialog opens. Verify the

5. The screen displays the update progress. When the update is complete, the

Set date and time

To ensure accurate date and time information on saved image files, verify your

5. Click OK in the Settings dialog. The new settings take effect immediately.

revision details, then click Yes to start the update.

main EVOS

™

screen reappears.

IMPORTANT! Do not power off the instrument, unplug the USB flash drive, or add or remove any devices during the update.

date/time settings on the EVOS™ XL Imaging System before your capture session.

1. Click Settings to open the Settings dialog, then select the Service tab.

2. Click the Change button under Date and Time in the Service tab.

3. In the Date an d Time dialog, use the arrows and the dropdowns to set the time, date, and time zone.

4. Click OK in the Date and Time dialog.

EVOS™ XL Imaging System User Guide

• When cleaning optical elements, use only optical-grade materials to avoid

Clean each objective periodically or when necessary with an optical-grade swab

•

5. Care and maintenance

General care

scratching soft lens coatings.

• Use the appropriate cleaning solutions for each compon ent, a s described in the “Decontamination procedures” (page 34).

• If liquid spills on the instrument, turn off the power immediately a nd wipe dry.

• Do not exchange objectives between instruments unless you know that the

components have been approved and recommended by Thermo Fisher Scientific.

• After using, cover the instrument with the supplied dust cover.

IMPORTANT! Never disassemble or service the instrument yourself. Unauthorized repairs may damage the instrument or alter its functionality, which may void your warranty. Contact your local EVOS arrange for service.

™

distributor to

Objective lens care

and a pre-moistened lens wipe (or lens paper moistened with lens cleaning solution). To avoid scratching the soft lens coatings, use only optical-grade

cleaning materials and do not rub the lens.

Stage care

Clean the stage as needed with paper towels or Kimwipes™ laboratory wipes dampened with 70% ethanol.

• When moving EVOS™ XL Imaging System, l ock the stage w ith the sta ge lock pin to prevent th e s tage from sli ding (page 4).

Note: To protect all optical components of the instrument, use the dust cover when the instrument is not in use.

EVOS™ XL Imaging System User Guide

Decontamination procedures

To decontaminate the EVOS™ XL Imaging System, follow these procedures:

1. Turn power OFF.

2. Clean the LCD display.

a. Use a soft, dry, lint-free cloth to wipe off any dust from the screen.

b. Clean the LCD display with a non-alcohol based cleaner made for flat-

panel displays.

IMPORTANT! Do not spray cleaning fluid directly onto the screen, as it may drip into the display or optics.

3. Lightly wipe the working surfaces of the instrument (stage top, focus knobs, objective selection wheel, housing) with paper towels or Kimwipes laboratory wipes dampened with 70% ethanol or 4,000 ppm hydrogen peroxide (H

4. If it is necessary to decontaminate the condenser, do not apply the decontamination solution directly to the condenser assembly. Instead, select the desired phase ring, and then cover the condenser with clear plastic wrap and wipe the wrap with decontamination solution.

™

2O2

IMPORTANT! Do not allow decontamination solution to get into lubricated areas, such as the stage roller bearings, or any points of rotation such as axles for the stage knobs, condenser wheel, etc. Do not soak any surface in decontamination solution. NEVER spray

™

liquid anywhere on the EVOS

XL Imaging System. Always wipe

surfaces with dampened paper towels instead.

IMPORTANT! EVOS UV sterilization. UV degrades many materials, including plastic.

™

XL Imaging System should not be subjected to

Damage from UV exposure is not covered under the manufacturer’s warranty.

EVOS™ XL Imaging System User Guide

Appendix A: Troubleshooting

Note: For additional technical support, contact your local EVOS

your distributor information, you can look it up at the thermofisher.com/evos or contact Technical Support (page 55).

Image quality issues

Problem Possible solutions

Image is too dim (at h igher magnifications)

• Set the phase annuli selector to the BF position.

• Remove condenser slider, if one is in place.

™

distributor. If you do not have

Specks, dots, or blurs on image

Uneven focus across screen

Difficulty focusing on coverslipped sample on standard

slide

LCD screen is black

LCD screen is red, or red patches cover parts of the screen

• Follow instructions under “Objective lens care” (page 33) to clean objectives.

• Position sample so that it lies flat on the stage; be sure the sample’s thickness is even.

• Place the slide so the coverslip is facing up (long working-distance objectives require a thick optical substrate, and image best

through 1.0–1.5 mm of glass or plastic).

• Click the LIGHT ON button (onscreen).

• Move objective selection wheel so that light shines through

objective.

• Verify that the phase annuli selector on condenser is not stuck between settings.

• Center sample over objective.

• Verify power supply is connected and power switch is on.

• Dim the illumination until the red highlights disappear to get the

maximum level of brightness without any overexposed areas.

• Disable the “Highlight saturated pixels in red” option in the Settings: Basic tab.

EVOS™ XL Imaging System User Guide

Software interface issues

We recommend keeping your EVOS software” (page 31) for more information.

Problem Possible solutions

Image does not respond to changes in focus or stage position

LIGHT ON/OFF button is inactive

™

instrument up to date with the latest software. See “Update the

• Click the LIGHT ON button to return to live observation (note

• Verify that the objective selection wheel is clicked into a position.

that a red USB icon on the Save button indicates there is an unsaved image, which will be lost unless it is saved before clicking LIGHT ON).

Scale bar does not appear when clicked

Save button does not respond when clicked

DVI output does not work on an external LCD monitor or projector

Unable to connect to network

Mechanical issues

Problem Possible solutions

Mechanical stage does not move

• Capture image first; scale bar is only available after capturing an image.

• Click capture first; It is only possible to save an image that is captured. The USB icon on the Save button is red when there is a

captured image to save.

• Power EVOS™ off, check the cable connections, and power on the instrument.

• Verify that the external display accepts DVI-D input (i.e. digital input). A monitor with analog input only (DVI-A) will not display the EVOS

™

’ digital output.

• Verify physical cable connections; confirm the Ethernet jack is active.

• Use a compatible network adapter.

• If the physical connections are good, and the problem persists,

contact your network administrator to resolve any network issues. Note that a Linux network must use Samba for the EVOS

™

Imaging System to be able to find it.

• Remove stage lock pin.

• Check stage brakes (on the stage knobs) and loosen as needed.

Vessel does not sit securely on moving stage

Mechanical stage drifts during time lapse sessions

Mechanical stage tension is loose

• Use the correct vessel holder for the application (refer to the

™

EVOS

Vessel Holders spec sheet, included on the USB flash drive. This information is also available at thermofisher.com/evosxl or from Technical Support (page 55).).

• Tighten the stage brakes (on the stage knobs) before starting a time lapse session (page 20).

• Tighten the stage brakes (on the stage knobs) as des ired to increase tension.

EVOS™ XL Imaging System User Guide

Appendix B: System specifications

Note: System specifications are subject to change without notice. Go to the EVOS

System product page (thermofisher.com/evosxl) to download the latest production information.

Optics

Objectives

Objective turret

Illumination

Contrast methods

Condenser

Condenser working distance

Mechanical “glide” stage

LCD display

Camera

Image acquisition

Captured images

Output ports

Power supply

Dimensions

Infinity-corrected optical system; RMS-threaded objectives with 45 mm parfocal distance

Objectives included vary per order. Contact your EVOS Support (page 55) for a full list of objectives supported.

5-position; front-mounted control

LED (50,000+ hour life); adjustable intensity

Brightfield and phase contrast

Includes 3-pos with brightfield and phase contrast annuli

60 mm

• X-Y axis fine-positioning controls; 28.3 mm (1.11 in) per rotation; 110 mm × 110 mm (4.3 in × 4.3 in) range of motion

• Z-axis focusing controls, 480 mm/rotation

• Interchangeable vessel holders available for most common shapes and sizes

15-inch color, 1024 × 768 pixels; adjustable tilt

1/2-inch 2048 × 1536, 3.1 Megapixels COLOR

Onboard microprocessor; built-in software for image acquisition via mouse control

24-bit/px, full color TIFF, PNG, JPG or BMP (2048 × 1536) pixels

3 USB and 1 DVI

AC Adapter; Input 100–240 V, 50 –60 Hz; Output 12 VDC/4.15 A

Operating height: 57.8 cm (22.75 in)

Storage/transport height: 32.4 cm (12.75 in)

Depth: 47.0 cm (18.5 in) Width: 35.5 cm (14.0 in)

™

XL Imaging

™

distributor or Technical

Weight

EVOS™ XL Imaging System User Guide

14.2 kg (31.4 lbs)

The Login button (Thermo Fisher Scientific logo,

Left control bar

The following functions are available in the left

Adjust color dialog

The Color adjustment button (left control bar)

Right Control Bar

The following functions are available in

Appendix C: Controls glossary

On screen controls

Note: This glossary is not alphabetized. On screen controls are listed from left to right.

bottom left) allows you to log in and create or change user profiles. This button also displays the current user profile (in this example, “Guest”).

control bar (bottom left):

• LIGHT ON button

• Illumination slider

• Image capture button

• Color Adjustment button

opens the Adjust color dialog where you can fine tune your live image Brightness, Contrast, Satura tion, and Hue prior to capture.

the right control bar (bottom right):

• View: Scale bar option

• View: Toolbar option

• Settings button

• Save button

• QuickSave option

• Selected objective indicator

EVOS™ XL Imaging System User Guide

View: Scale bar option

The Scale bar option (right control bar) is

View: Toolbar option

The Toolbar option (right control bar)

Time lapse tool

The Time lapse tool allows you to set up

Count tool

The Count tool allows you to tag cells and

Image review tool

The Image review tool allows you to

Settings button

The Settings button (right control bar) opens the Settings dialog.

a toggle button that displays or hides the Scale bar tool. This option is only available after an image is captured. To move the Scale bar, click and drag it.

includes the Time Lapse, Count, and Image review tools. Click the small gray triangle to open each tool.

the interval/duration and the file name/ destination for time lapse sessions. See

Record time lapse images”, page 20.

“

other features using up to 6 labels, and it calculates the totals and percentages for each label. See “

Count cells”, page 23.

review still images or play video files from the USB drive or network connection and to rena me or del ete saved files . See “

Review

images”, page 24.

Within the Settings dialog, the following options are available:

• Basic tab

• QuickSave tab

• Network tab

• Service tab

EVOS™ XL Imaging System User Guide

Settings: Basic tab

•

Settings: QuickSave tab

Allows you to set up a file name, count, file type, and folder

Network tab

(Not shown)

Service tab

(Not shown)

Save button

The Save button (right control bar) saves the current image to the

This EVOS™ section displays the serial number and

software version.

• Login section controls default Guest login.

• Display section controls the following options:

• Highlight saturated pix els in red indicates overexposed

areas onscreen;

• Reset Scale bar Location moves the Scale bar back to its default position in the lower right corner of the screen;

• Enable Mouse Wheel allows the mouse scroll wheel to control illumination levels;

• Mouse Speed controls the mouse’s reaction time;

• LCD Backlight controls the LCD lighting.

• File Save section allows you to specify 8-bit as the

default format for TIFF files.

to save captured images automatically.

Allows you to set up connections to shared folders via

™

Ethernet. See “Connect the EVOS

XL Imaging System to a

network”, page 28.

Allows for maintenance functions; contact your distributor if service is needed.

USB flash drive or network folder. A red USB icon indicates that the current image has not been saved yet. After a file is saved, the USB icon turns blue.

EVOS™ XL Imaging System User Guide

Save File dialog

Virtual keyboard

The Virtual keyboard allows you enter text anytime a field

QuickSave option

The QuickSave option (right control bar) shows the QuickSave option

Selected objective

The Sele cted Objective display (right control bar) shows the

Digital Zoom Value display

The Save File dialog allows you to name and save captured image files. The following options are available in the Save File dialog:

• Save File Name field: Click to enter a file name.

• New Folder button: Click to enter a folder name and date (optional).

• Save Folder list: The captured image will save to the selected (blue) folder.

• File list: (to the right of the Save Folder list) Displays saved files already in the selected folder.

• File type menu: Dropdown allows .tif, .png, .jpg, or .bmp formats.

• Comment field: Click to enter a comment and date (optional).

or text-related button is selected. Where applicable, a Date button is available to include the current date in the field. Click the Accept button when you have finished entering the text.

Note: You can connect a USB keyboard to the EVOS™ XL Imaging System for text input. When a keyboard is connected, the virtual keyboard will not appear on screen except when you add a new user ID or rename a file with the Image review tool.

button with the current QuickSave file name. If no QuickSave base file name is set up, this space will appear blank. For more information, see “QuickSave tab” under “Settings button”, page

40.

magnification currently in use.

The Digital Zoom Value display (right control bar) appears above

the Selected Objective display to show the zoom value when you zoom in on an image.

EVOS™ XL Imaging System User Guide

Selected objective

Objective turret

between positions

Mechanical controls

Power input jack

Power switch

USB and DVI ports

Coarse stage

Use the coarse stage positioning knobs to position the sample within the field of

Stage X-axis knob

Use the stage X-axis knob for fine left-right movements to position the sample

Power switch

Power input jack

USB and DVI ports

Coarse stage positioning knobs

Stage X-axis knob

Plug the power adapter into the power input jack.

Set the power switch to “—” to turn the instrument on or “O” to turn it off.

Plug the mouse and flash drive into the USB ports. Use the DVI port for digital output to a projector or other display.

positioning knobs

view, particularly at low magnifications.

within the field of view, particularly at high magnifications. To secure the stage in place for time-lapse image captures, tighten the X-axis stage brake.

Objective selection wheel

Focus knobs

Stage Y-axis knob

Phase annuli selector

Condenser slider slot

EVOS™ XL Imaging System User Guide

Stage Y-axis knob

Use the stage Y-axis knob for fine front-back movements to position the sample

Focus knobs

Use the focus knobs to bring the sample into focus.

Objective selection

Turn the objective selection wheel to change magnifications. The objective turret

Phase annuli

Set the phase annuli selector to the position that corresponds with your selected

Condenser slider

Enhance image quality by inserting a condenser slider all the way into the slot.

Mouse scroll wheel

When the “Enable Mouse Wheel” option is selected in the Basic tab of the Settings

within the field of view, particularly at high magnifications. To secure the stage in place for time-lapse image captures, tighten the Y-axis stage brake.

wheel

selector

slot

clicks into place at each position.

objective for transmitted light observations. The selector clicks into place for each of the following positions:

• BF (use for brightfield observations)

• 4/10 PH (use for phase observations at 4x or 10x)

• 20/40x PH (use for phase observations at 20x or 40x)

dialog, the mouse scroll wheel can be used to adjust the illumination intensity. Roll the scroll wheel away from you for brighter illumination, or roll it toward you for dimmer illumination.

EVOS™ XL Imaging System User Guide

Appendix D: Safety

This section includes the following topics:

• Safety conventions used in this document

• Symbols on instruments

• Safety labels on instruments

• General instrument safety

• Chemical safety

• Chemical waste safety

• Electrical safety

• Physical hazard safety

• Biological hazard safety

• Workstation safety

• Safety and electromagnetic compatibility (EMC) standards

• SDSs

EVOS™ XL Imaging System User Guide

Safety alert words

Four safety alert words appear in Thermo Fisher Scientific user documentation at

Definitions

Except for IMPORTANT! safety alerts, each safety alert word in a Thermo Fisher

Thermo Fisher Scientific instruments (see “Safety symbols” on page 47).

Safety conventions used in this document

points in the document where you need to be aware of relevant hazards. Each alert word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular level of observation or action:

IMPORTANT! Provides information that is necessary for proper instrument operation, accurate installation, or safe use of a chemical.

CAUTION! – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

WARNING! – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury.

DANGER ! – Indicates an imminently hazardous situation that, if not avoided, will result in death or serious injury. This signal word is to be limited to the most extreme situations.

Scientific document appears with an open triangle figure that contains a hazard symbol. These hazard symbols are identical to the hazard icons that are affixed to

EVOS™ XL Imaging System User Guide

Symbols on instruments

Electrical symbols

The following table describes the electrical symbols that may be displayed on

on instruments

Thermo Fisher Scientific instruments.

Symbol Description

Indicates the On position of the main power switch.

Indicates the Off position of the main power switch.

Indicates a standby switch by which the instrument is switched on to the Standby condition. Hazardous voltage may be present if this switch is on standby.

Indicates the On/Off position of a push-push main power switch.

Indicates a terminal that may be connected to the signal ground reference of another instrument. This is not a protected ground terminal.

Indicates a protective grounding terminal that must be connected to earth ground before any other electrical connections are made to the instrument.

Indicates a terminal that can receive or supply alternating current or voltage.

Indicates a terminal that can receive or supply alternating or direct current or voltage.

EVOS™ XL Imaging System User Guide

Safety symbols

The following table describes the safety symbols that may be displayed on

support documents.

Environmental

The following symbol applies to all Thermo Fisher Scientific electrical and

Thermo Fisher Scientific instruments. Each symbol may appear by itself or in combination with text that explains the relevant hazard (see “ instruments”, page 48

WARNINGS, and CAUTIONS that occur in the text of this and other product-

Symbol Description

Indicates that you should consult the manual for further information and to proceed with appropriate caution.

Indicates the presence of an electrical shock hazard and to proceed with appropriate caution.

Indicates the presence of a hot surface or other high-temperature hazard and to proceed with appropriate caution.

Indicates the presence of a laser inside the instrument and to proceed with appropriate caution.

Indicates the presence of moving parts and to proceed with appropriate caution.

Indicates the presence of a biological hazard and to proceed with appropriate caution.

). These safety symbols may also appear next to DANGERS,

Safety labels on

symbols on instruments

Indicates the presence of an ultraviolet light and to proceed with appropriate caution.

electronic products placed on the European market after August 13, 2005.

Symbol Description

Do not dispose of this product as unsorted municipal waste. Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment (WEEE).

European Union customers:

Call your Customer Service representative for equipment pick-up and recycling. See www.thermofisher.com for a list of customer service offices in the European Union.

EVOS™ XL Imaging System User Guide

Safety labels on instruments

The following CAUTION, WARNING, and DANGER statements may be displayed on

described in the preceding section.

Thermo Fisher Scientific instruments in combination with the safety symbols

Hazard

symbol

English Français

CAUTION! Hazardous chemicals. Read the

Safety Data Sheets (SDSs) before handling.

CAUTION! Hazardous waste. Refer to SDS(s) and local regulations for handling and

disposal.

DANGER ! High voltage. DANGER ! Haute tension.

WARNING! To reduce the chance of electrical

shock, do not remove covers that require tool access. No user-serviceable parts are inside. Refer servicing to Thermo Fisher Scientific qualified service personnel.

DANGER ! Class 3B visible and/or invisible laser radiation present when open. Avoid exposure to beam.

CAUTION! Moving parts. Crush/pinch hazard.

ATTENTION! Produits chimiques dangereux. Lire les fiches techniques de sûreté de matériels avant toute manipulation de produits.

ATTENTION! Déchets dangereux. Lire les fiches techniques de sûreté de matériels et la régulation locale associées à la manipulation

et l’élimination des déchets.

AVERTISSEMENT! Pour éviter les risques d’électrocution, ne pas retirer les capots dont l’ouverture nécessite l’utilisation d’outils. L’instrument ne contient aucune pièce réparable par l’utilisateur. Toute intervention doit être effectuée par le personnel de service qualifié venant de chez Thermo Fisher

Scientific.

DANGER ! Rayonnement visible ou invisible d’un faisceau laser de Classe 3B en cas d’ouverture. Evitez toute exposition au faisceau.

ATTENTION! Pièces en mouvement, risque de pincement et/ou d’écrasement.

EVOS™ XL Imaging System User Guide

Moving and lifting

side and hold it stationary while someone slides the contents out of the box.

Operating the

Ensure that anyone who operates the instrument has:

Data Sheets (SDS)”, page 55.

Cleaning or

General instrument safety

WARNING! PHYSICAL INJURY HAZARD. Use this product only as specified

in this document. Using this instrument in a manner not specified by Thermo Fisher Scientific may result in personal injury or damage to the instrument.

CAUTION! PHYSICAL INJURY HAZARD. The instrument is to be moved and

the instrument

stand-alone computers and monitors

positioned only by the personnel or vendor specified in the applicable site preparation guide. If you decide to lift or move the instrument after it has been installed, do not attempt to lift or move the instrument without the assistance of others, the use of appropriate moving equipment, and proper lifting techniques. Improper lifting can cause painful and permanent back injury. Depending on the weight, moving or lifting an instrument may require two or more persons.

WARNING! Do not attempt to lift or move the computer or the monitor without the assistance of others. Depending on the weight of the computer and/or the monitor, moving them may require two or more people.

Things to consider before lifting the computer and/or the monitor:

• Make sure that you have a secure, comfortable grip on the computer or the monitor when lifting.

• Make sure that the path from where the object is to where it is being moved is clear of obstructions.

• Do not lift an object and twist your torso at the same time.

• Keep your spine in a good neutral position while lifting with your legs.

• Participants should coordinate lift and move intentions with each other before

lifting and carrying.

• Instead of lifting the object from the packing box, carefully tilt the box on its

instrument

decontaminating the instrument

• Received instructions in both general safety practices for laboratories and specific safety practices for the instrument.

• Read and understood all applicable Safety Data Sheets (SDSs). See “Safety

CAUTION! Using cleaning or decontamination methods other than those recommended by the manufacturer may compromise the safety or quality of the instrument.

EVOS™ XL Imaging System User Guide

Chemical safety

Chemical hazard

General safety

To minimize the hazards of chemicals:

warning

WARNING! CHEMICAL HAZARD. Before handling any chemicals, refer to

the Safety Data Sheet (SDS) provided by the manufacturer, and observe all relevant precautions.

WARNING! CHEMICAL HAZARD. All chemicals in the instrument, including liquid in the lines, are potentially hazardous. Always determine what chemicals have been used in the instrument before changing reagents or instrument components. Wear appropriate eyewear, protective clothing, and gloves when working on the instrument.

WARNING! CHEMICAL STORAGE HAZARD. Never collect or store waste in a glass container because of the risk of breaking or shattering. Reagent and waste bottles can crack and leak. Each waste bottle should be secured in a low-density polyethylene safety container with the cover fastened and the handles locked in the upright position. Wear appropriate eyewear, clothing, and gloves when handling reagent and waste bottles.

guidelines

• Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials. (See “Safety Data Sheets (SDS)”, page 55.)

• Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). For additional safety guidelines, consult the SDS.

• Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood). For additional safety guidelines, consult the SDS.

• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer’s cleanup procedures as recommended in the SDS.

• Comply with all local, state/provincial, or national laws and regulations related to chemical storage, handling, and disposal.

EVOS™ XL Imaging System User Guide

Chemical waste

To minimize the hazards of chemical waste:

environmental and health regulations.

Waste disposal

If potentially hazardous waste is generated when you operate the instrument, you

regulations.

Chemical waste safety

CAUTION! HAZARDOUS WASTE. Refer to Safety Data Sheets (SDSs ) and

hazard

local regulations for handling and disposal.

safety guidelines

• Read and understand the Safety Data Sheets (SDSs) provided by the manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste.

• Provide primary and secondary waste containers. (A primary waste container holds the immediate waste. A secondary container contains spills or leaks from the primary container. Both containers must be compatible with the waste material and meet federal, state, and local requirements for container storage.)

• Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood). For additional safety guidelines, consult the SDS.

• Handle chemical wastes in a fume hood.

• After emptying the waste container, seal it with the cap provided.

• Dispose of the contents of the waste tray and waste bottle in accordance with

good laboratory practices and local, state/provincial, or national

must:

• Characterize (by analysis, if necessary) the waste generated by the particular applications, reagents, and substrates used in your laboratory.

• Ensure the health and safety of all personnel in your laboratory.

• Ensure that the instrument waste is stored, transferred, transported, and

disposed of according to all local, state/provincial, and/or national

IMPORTANT! Radioactive or biohazardous materials may require special handling, and disposal limitations may apply.

EVOS™ XL Imaging System User Guide

Electrical safety

‐

Fuses

Power Overvoltage rating

The EVOS™ XL Imaging System has an installation (overvoltage) category of II,

DANGER ! ELECTRICAL SHOCK HAZARD. Severe electrical shock can result from operating the EVOS panels in place. Do not remove instrument panels. High are exposed when instrument panels are removed from the instrument.

WARNING! FIRE HAZARD. For continued protection against the risk of fire, replace fuses only with fuses of the type and rating specified for the

instrument.

DANGER ! ELECTRICAL HAZARD. Grounding circuit continuity is vital for the safe operation of equipment. Never operate equipment with the

grounding conductor disconnected.

DANGER! ELECTRICAL HAZARD. Use properly configured and approved line cords for the voltage supply in your facility.

DANGER! ELECTRICAL HAZARD. Plug the system into a properly grounded receptacle with adequate current capacity.

™

XL Imaging System without its instrumen t

voltage contacts

and is classified as portable equipment.

EVOS™ XL Imaging System User Guide

Moving parts

Physical hazard safety

WARNING! PHYSICAL INJURY HAZARD. Moving parts can crush and cut.

Keep hands clear of moving parts while operating the instrument. Disconnect power before servicing the instrument.

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids,

and blood of humans and other animals have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations. Wear appropriate protective eyewear, clothing, and gloves. Read and follow the guidelines in these publications:

In the U.S.:

• U.S. Department of Health and Human Services guidelines published in

Biosafety in Microbiological and Biomedical Laboratories

(stock no. 017-040-00547-4; www.cdc.gov/OD/ohs/biosfty/bmbl4/bmbl4toc.htm)

• Occupational Safety and Health Standards, Bloodborne Pathogens

(29 CFR§1910.1030; www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html)

• Your company’s/institution’s Biosafety Program protocols for working with/handling potentially infectious materials.

• Additional information about biohazard guidelines is available at:

www.cdc.gov

In the EU:

• Check your local guidelines and legislation on biohazard and biosafety precaution, and the best practices published in the World Health Organisation (WHO) Laboratory Biosafety Manual, third edition

www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_ 2004_11/en/

EVOS™ XL Imaging System User Guide

This section provides information on:

Australian EMC standards

U.S. and Canadian

The CSA C/US Mark signifies that the product meets applicable U.S. and

European Safety

The CE Mark symbolizes that the product conforms to all applicable European

Australian EMC

The C-Tick Mark indicates conformity with Australian and New Zealand

Safety and electromagnetic compatibility (EMC) standards

• U.S. and Canadian safety standards

• European safety and EMC standards

•

Safety Standards

and EMC Standards

standards

Canadian standards, including those from CSA, CSA America, ANSI, ASME, ASSE, ASTM, NSF and UL.

Community provisions for which this marking is required. Operation of the instrument is subject to the conditions described in this manual. The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Thermo Fisher Scientific.

standards for electromagnetic compatibility.

EVOS™ XL Imaging System User Guide

Technical support

For the latest services and support information for all locations, visit

Download software updates and patches

Safety Data Sheets

Safety Data Sheets (SDSs) are available at thermofisher.com/sds.

Limited product

Life Technologies Corporation and/or its affiliate(s) warrant their products as set

at www.thermofisher.com/support.

Documentation and support

Obtaining support

www.thermofisher.com.

At the website, you can:

• Access worldwide telephone and fax numbers to contact Technical Support and Sales facilities

• Search through frequently asked questions (FAQs)

• Submit a question directly to Technical Support (thermofisher.com/support)

• Search for user documents, SDSs, vector maps and sequences, application

notes, formulations, handbooks, certificates of analysis, citations, and other product support documents

• Obtain information about customer training

•

(SDS)

IMPORTANT! For the SDSs of chemicals not distributed by Thermo Fisher

Scientific contact the chemical manufacturer.

warranty

forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at

and-conditions.html

IMPORTANT! Wiping the EVOS™ XL Imaging System computer (i.e., erasing the hard drive to remove all programs, files, and the operating

system) voids the product warranty.

. If you have any questions, please contact Life Technologies

www.thermofisher.com/us/en/home/global/terms-

EVOS™ XL Imaging System User Guide

thermofisher.com/support | thermofisher.com/askaquestion

thermofisher.com

09 October 2017

Invitrogen Evos XL User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual