Invitrogen Biomarker quantitation Instruction Manual

Biomarker quantitation assay guide

Quantitate protein: ELISA l Antibody pairs l ProQuantum HS immunoassays l ProcartaPlex immunoassays

Quantitate mRNA: QuantiGene multiplexed gene expression assays

The Invitrogen™ portfolio oers a variety of assays for quantitation of single proteins and mRNA as well as mix-and-match and ready-to-use panels for correlated multiplexing using the Luminex extensive selection of Invitrogen

platform. Our

™

assays includes:

• ELISA and antibody pair kits

• ProQuantum

• ProcartaPlex

• QuantiGene

™

high-sensitivity immunoassay kits

™

multiplex immunoassay kits

™

Plex gene expression assays

Additionally, Thermo Fisher Scientiﬁc supports your quantitation assay needs with accessory reagents and instruments for a comprehensive oering. Use this guide to help identify your needs, then contact your sales representative if you have questions or go to thermoﬁsher.com/quantitatebiomarkers.

Contents

Biomarker background 4

Biomarker quantitation assay platforms 7

Single-analyte quantitation

ELISA kits 9

Antibody pair kits 11

Accessory reagents and equipment 12

ProQuantum high-sensitivity immunoassay kits 14

Multianalyte quantitation

Luminex xMAP technology 17

ProcartaPlex multiplex immunoassay kits 19

ProcartaPlex high-sensitivity immunoassay kits 28

Immunoassay Special Services 29

ProcartaPlex mix-and-match panels 30

Multiplexed gene expression assays 32

QuantiGene Plex assays 32

Additional gene expression solutions 38

Comprehensive immunoassay product listing 40

Biomarker background

Highly referenced kits you can trust

We proudly oer a selection of quantitation assays for the measurement of protein and RNA as biomarkers in key disease research areas. From single-analyte analysis through multiple ELISA formats to multiplex quantitation using Invitrogen™ ProcartaPlex™ and QuantiGene™ Plex assays for the Luminex® platform, you can study the mechanisms of disease pathways from expression of the gene to the protein.

This guide describes all of the options available for singleanalyte and multianalyte assays, as well as showing the correlation between these assays. With high correlative values between these various assays, you have the ﬂexibility to use the assay you need to continue your research and publish with conﬁdence.

Assays within these areas of research assess cellular mechanisms from basic drug discovery to development

• Adaptive and innate immunity

• Adhesion and migration

• Angiogenesis

• Apoptosis

• Bone biology

• Cancer biology and immuno-oncology

• Cardiac proteins for arteriosclerosis and atherosclerosis

• Cell cycle and proliferation

• Cell signaling and cell structure

• Cytokines, chemokines, and growth factors

• Metabolism

• Neurobiology

• Stem cell biology

• Transcription factors

The importance of biomarker quantitation assays

As part of the immune response, inﬂammation plays an important role in defending the body against pathogens, such as viruses, bacteria, fungi, and other parasites. However, inappropriate activation of inﬂammatory processes is an underlying contributor to many common pathological conditions. For example, autoimmune conditions arise when our immune system mistakes our cells or tissues for non-self antigens and attacks them. In addition, studies show that tumor proliferation and metastasis may occur when inﬂammatory cytokines create a microenvironment conducive to cancer progression. Figure 1 shows a simpliﬁed representation of cytokine interactions during inﬂammation.

Acute versus chronic inﬂammation

Acute inﬂammation is a short-lived response that is characterized by extravasation of leukocytes, erythrocytes, and plasma components into the injured tissue. If left unchecked, the acute inﬂammatory process can lead to chronic inﬂammation.

Unlike acute inﬂammation, chronic inﬂammation is characterized primarily by tissue inﬁltration of lymphocytes and macrophages. Chronic inﬂammation is closely associated with allergy, atherosclerosis, cancer, arthritis, and Alzheimer’s disease, as well as autoimmune diseases. The process of acute inﬂammation is well deﬁned, but the causes of chronic inﬂammation and its associated molecular and cellular pathways are still not well understood [1].

Inﬂammation research

Acute phase

proteins

Fever

Neutrophil elastase

Respiratory burst

Reactive oxygen species

Resting B cell

Activated B cell

Plasma cell

IgM/G/A

Humoral immunity

Fibroblast

liver

Neutrophil

Allergy anaphylaxis

I L- 8,

TNF-α

IFN-γ

TGF-β, IL-2,

I L- 4, I L-5 , I L-13 ,

IFN-γ

IgE

Bacterial products

(LPS, peptidoglycans, etc.)

TNF-α I L-1 β TGF-β PDGF I L- 6

Macrophage

I L-1 , IL- 6, IL- 8 , IL-10 ,

IL-12, IL-15, IFN-α, IFN-β,

TNF-α, M C P-1, MIP -1α

I L- 3 , I L-4 , I L-10,

IL-13, IFN-γ, T NF-β

I L-1 0 , IL- 14 ,

I L- 4, IL- 6

I L- 6

Th1/Th2

Viral infection,

tumor surveillance

Resting T cell

Activated

T cell

I L- 2

Th17/22

IL-2, IFN-γ, I L- 4

T N F, I L-1 β

M-CSF, TNF-α, G M- C S F,

G - CS F, I L-1 , IL- 6, I L-11 ,

LFA-1, VLA-4,

I L- 2 3

Endothelial

I L-1 2

I L- 3 , I L-6 ,

IL-7, GM-CSF

Mac -1

CTL

cell

I L- 3 , I L-4 ,

I L-1 0

I L- 3 , I L-4 , I L- 5

Lung

epithelium

Fibrosis

I L-1 7A , I L-1 7 F,

I L- 2 2 , I L-6 ,

GM-CSF

PE C AM-1, VCA M -1, ICAM-1, P-selectin,

Hematopoiesis

Th9

E-selectin

I L-1 8

I L-1 2 , I L-15

Eotaxin

I L- 2

Cardiovascular

pathology

Histamine

Mast cell

Eosinophil

NK cell

Autoimmunity

Tissue repair,

protozoan immunity

Allergy anaphylaxis

Innate immunity

Viral infection,

tumor surveillance

Figure 1. Interaction of cytokines during inﬂammation.

The critical balance between pro- and anti-inﬂammatory mediators

The overall eect of an inﬂammatory response is dictated by the balance between pro- and anti-inﬂammatory mediators (Table 1).

Pro-inﬂammatory cytokines such as IL-1β, IL-6, and TNF-α, are responsible for early responses and amplify inﬂammatory reactions, whereas anti-inﬂammatory cytokines, which include IL-4, IL-10, and IL-13, have the opposite eect in that they limit the inﬂammatory reactions (Table 1). The increasing complexity of pro- and

Table 1. Pro- and anti-inﬂammatory mediators [2].

Pro-inﬂammatory mediators

• Cell-surface CD14 • Leukemia inhibitory factor • Prostacyclins

• Free radical generation • Monocyte chemoattractant protein-1 • Prostaglandins

• IL-1β • Monocyte chemoattractant protein-2 • Protein kinase

• IL-2 • Neopterin • Soluble adhesion molecules

• IL- 6 • Neutrophil elastase • Thromboxane

• IL- 8 • Phospholipase A2 • TNF-α

• IL-15 • Plasminogen activator inhibitor-1 • Tyrosine kinase

• IFN-γ • Platelet activating factor • Vasoactive neuropeptides

Anti-inﬂammatory mediators

• Epinephrine • Leukotriene B4 receptor antagonists • Soluble TNF-α receptors

• IL- 4 • LPS binding protein • Transforming growth factor-β

• IL-10 • Soluble recombinant CD-14 • Type II IL-1R

• IL-13

• IL-1R A

anti-inﬂammatory cytokine and chemokine networks has made it crucial to examine them in relevant functional groups rather than individually (Figure 1, page 5).

To facilitate the investigation of inﬂammatory and other biologically relevant biological markers, the Invitrogen™ portfolio has a broad menu of immunoassays to easily detect and quantify proteins, and to facilitate tracking gene expression of RNAs with the QuantiGene Plex assays. Products are available for important research areas, including immunology, neurobiology, cancer, cardiovascular diseases, metabolism, and more.

Plate- and bead-based solutions for your research needs across many species

Human, mouse, rat, nonhuman primate, canine, porcine, bovine, equine, and feline

The Invitrogen portfolio of immunoassay products supports the evolution of research projects by oering multiple platforms that provides the freedom to scale from ELISA to multiplex assay based on project needs. Adding QuantiGene Plex assays to the workﬂow can provide an accurate and precise method for multiplexed gene expression quantitation, using Luminex® xMAP® technology.

• Keep your research relevant by incorporating analytes pertinent to your area of work at any point within your study

• Conserve resources by using existing data from previous experiments

Biomarker quantitation assay platforms

Tailored to meet your needs

A variety of ELISA kit formats are available that include complete, ready-to-use kits as well as preoptimized reagents to make your own. A variety of singleplex and multiplex panels for the Luminex platform are also available. See Table 2 for a performance comparison of our immunoassay platforms and Table 3 for a component list.

• Invitrogen™ coated ELISA kits—Quantitate with conﬁdence. Highly veriﬁed ELISA kits with precoated plates provide lower inter- and intra-assay variability with ready-to-use reagents that help ensure consistent data.

– Invitrogen™ Instant ELISA kits—Fewer steps and

hands-on time to increase productivity. The one-wash ELISA introduces fewer handling steps.

– Invitrogen™ phosphospeciﬁc ELISA kits—Measure

phosphospeciﬁc proteins in cell lysates.

• Invitrogen™ antibody pair kits—Keep costs low with our aordable coat-it-yourself ELISA plate set. Each set contains the reagents required to prepare and run the

ELISA, including ELISA-optimized matched antibody pairs, standards, detection reagents, coating buers, sample diluent, and tetramethylbenzidine (TMB) substrate solution. Plates are optional.

• ProQuantum high-sensitivity (HS) assay kits— Take advantage of a platform innovation that provides researchers with an easy-to-run, high-performance assay, utilizing proximity-based ampliﬁcation technology to combine the analyte speciﬁcity of high-anity antibody–antigen binding with the signal detection and ampliﬁcation capabilities of real-time PCR to achieve a highly sensitive protein quantitation assay, using very small sample volumes.

• ProcartaPlex multiplex immunoassays—Quantitate more with less sample. ProcartaPlex multiplex immunoassays utilize Luminex xMAP technology for the biomarker proﬁling of up to 65 analytes in a single 25–50 μL sample.

Table 2. Characteristics of our immunoassay kits.

Type of immunoassay

Build-it-yourself

immunoassays

Ready-to-use reagents

Analytical sensitivity

Dynamic range

Sample volume 10 –10 0 µ L 10–100 µL 10 –100 µL 25–50 µL 2–5 µL 25–50 µL

Incubation time

Multiplexibility No No No No No Yes

Number of targets measured per well

Readout

Instrumentation needed

Instrument read time

* Values in this table refer to standard colorimetric kits. Ultrasensitive kits are also available. ** Immunoassays for the Luminex platform. † Every assay has its own speciﬁcations. Please consult the protocol for your speciﬁc immunoassays or kits.

†

No; need overnight

coating process

<10 pg/mL <10 pg/mL <10 pg/mL <5 pg/mL <0.1 p g/m L <10 pg/mL

†

<5–250 pg/mL <5–500 pg/mL <5–250 pg/mL <7. 8 – 50 0 p g /m L 0.064–5,000 pg/mL <5–2,000 pg/mL

†

4 hr 3–4.5 hr 2.5– 4 hr 4 hr 2 hr 3.5 hr

1 1 1 1 1 1–65

HRP-TMB

(colorimetric)

Microplate reader Microplate reader Microplate reader Microplate reader RT- PCR

2 min 2 min 2 min 2 min 36–72 min 20–60 min

Antibody pair

kits

Yes; ne e d

overnight coating

process

HRP-TMB

(colorimetric)

Coated

ELISA kits*

Yes Yes Yes Yes

HRP-TMB

(colorimetric)

Instant

ELISA kits

HRP-TMB

(colorimetric)

ProQuantum

HS kits

™

FAM

(ﬂuorescence)

Luminex (Luminex® 100/200™,

ProcartaPlex

immunoassays**

RPE

(ﬂuorescent)

instrument

MAGPIX®, or

FLEXMAP 3D®

system)

Go to thermoﬁsher.com/immunoassays to ﬁnd:

• Our helpful immunoassay selection guide that allows you to search for assays based on your target protein

• Detailed information on all of our antibody pair kits, ELISA kits, and ProQuantum high-sensitivity and

• Important data demonstrating our assay speciﬁcity and sensitivity

multiplex assay kits

Table 3. List of components for dierent immunoassay kits.

Type of immunoassay

Immunoassay components

96-well plate Optional • • NA Flat bottom

Pretitrated capture antibody

Pretitrated detection antibody

Recombinant protein standard

Stop solution Not included • • NA NA

Substrate solution • • • NA SA-PE

Assay buer • • Sample diluent • •

Antibody pair

ELISA kits

• Precoated Precoated • Precoated capture bead

• • • • •

• •

Coated

ELISA kits

Instant

ELISA kits

Additional well

strips included

ProQuantum HS

kits

• •

ProcartaPlex assays

(all platforms)

Wash buer Not included • • NA •

Coating buer • NA NA NA NA

Note: Our immunoassays are available in various kit sizes. Kits are calibrated to National Institute for Biological Standards and Control (N IBSC) or World Health Organization ( WHO) standards (if available). SA = streptavidin, PE = phycoerythrin, NA = not applicable.

Our ELISA kits are developed to meet speciﬁcations typically expected by customers, including standard calibration, precision, sensitivity, speciﬁcity, recovery, lot-to-lot consistency, linearity, and parallelism (Table 4).

Table 4. Rigorous assay veriﬁcation of ELISA kits helps ensure consistent, reliable results.

Speciﬁcation Description What does it mean for you?

Standard calibration

Precision Average inter-assay CV <10%; avg. intra-assay CV <10% Consistent results

Sensitivity

Speciﬁcity Cross-reactivity tests are performed with similar analytes

Recovery Buers are optimized to minimize matrix eects

Lot-to-lot consistency

Linearity of dilution

Calibrated to NIBSC, if available

Relevant levels of protein are detected for speciﬁc assay type

In-house controls are tested to measure within set ranges Helps to ensure consistent results with new lots

Linear results over the quantitative range of the assay Serial dilution of samples are quantitated accurately

Allows accurate quantitation and consistent standard of reference across multiple platforms

Enables detection of low levels of protein

Helps to ensure accurate measurement of the protein of interest

Helps to ensure accurate quantitation of samples within a complex matrix, including serum and plasma

Parallelism Recombinant protein standards mimic native proteins Samples can be measured with conﬁdence

Single-analyte quantitation : ELISA kits

Precoated

A complete, validated ELISA solution

Quantitate with conﬁdence

Coated ELISA kits for the quantitation of individual analytes from a variety of sample types provide lower inter- and intra-assay variability. Developed and manufactured for labs that require veriﬁed and comprehensively tested kits, coated ELISA kits oer lot-to-lot consistency and help ensure the highest possible ELISA performance (see an example standard curve in Figure 2). Ready-to-use reagents, including precoated plates, help ensure consistent data throughout your research.

Beneﬁts of precoated ELISA kits

• Robust—low inter- and intra-assay coecient of variation (CV)

• Easy to use—includes all required assay buers and reagents, and a straightforward protocol

• Highly veriﬁed—optimized for serum, plasma, and cell culture supernatants

Request bulk packaging or lot reservation, or go custom at

thermoﬁsher.com/IASpecialServices

2.2

2.0

1.8

1.6

1.4

1.2

1.0

0.8

Absorption (450 nm)

0.6

0.4

0.2

Figure 2. Standard curve for human IgG1-coated ELISA (Cat. No. BMS2092).

Human IgG1 Platinum ELISA

Concentration (ng/mL)

100 1,000

ELISA kits for protein analysis

Watch our videos “Using Invitrogen precoated ELISA kits” and “How to coat a plate with uncoated Invitrogen ELISA kits” to ﬁnd out all about running an ELISA to measure target proteins in serum, plasma, supernatants, cell lysates, and other sample types.

Watch ELISA videos at thermoﬁsher.com/elisahowtovideos

ELISA kits (continued)

Precoated

Instant ELISA: the one-wash ELISA kit

Reduce steps and hands-on time by 60%

The one-wash Instant ELISA kit requires fewer handling steps than the conventional ELISA kit (Table 5). The Instant ELISA kit contains all of the necessary components, including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. Additionally, the strip wells containing the standards for the standard curve are provided separately; they are ready to use, helping save both time and wells. Less hands-on time increases productivity, which leaves more time for your research.

Table 5. Steps for conventional ELISA vs. Instant ELISA kits.

17 steps for conventional ELISA

1 Washing of coated plate

Reconstitution of

standard protein Addition of sample diluent

to standard wells Titration of standard

curve

5 Addition of sample diluent 2 Sample addition 6 Sample addition 3 Incubation

Dilution of biotin

conjugate Addition of biotin

conjugate

9 Incubation

Preparation of

streptavidin-HRP conjugate

11 Washing step

Addition of streptavidin-

HRP conjugate

13 Incubation 14 Washing step 4 Washing step 15 Addition of TMB substrate 5 Addition of TMB substrate 16 Addition of stop solution 6 Addition of stop solution 17 Calculation of results 7 Calculation of results

7 steps for Instant ELISA kits

1 Rehydration of plate

Beneﬁts of Instant ELISA kits

• Time saving—only requires 15 min for setup

• Maximum accuracy—no need to add antibody or perform serial dilution of standards; reduced handling means less error and more consistent results

• Better value—generate standard curve data in parallel with additional well strips provided, to enable use of all 96 wells for your samples

Switching to Instant ELISA kits

The Instant ELISA kits utilize the same antibody pairs for many analytes across all assay formats. This enables cross-platform performance and comparable data using any of our Instant ELISA kits, ELISA, and ProcartaPlex multiplex assays. For example, if you are currently using an Invitrogen™ sCD44var Coated ELISA, you can be conﬁdent in switching to the Invitrogen™ sCD44var Instant ELISA assay, knowing that the data generated across the two kits will remain consistent (Figure 3).

0 10 20 30 40 50 60 70

sCD44var Coated ELISA (ng/mL)

Figure 3. Performance comparison of human sCD44var Instant ELISA to coated ELISA . Human sCD44var Instant ELISA kit

(Cat. No. BMS220INST) was tested against a human sCD44var Coated ELISA kit (Cat. No. BMS220). Sixteen serum samples were applied with the coecient of determination (R²) of 0.98, demonstrating comparable performance of the Instant ELISA kits against coated ELISA kits.

R2 = 0.98

sCD44var Instant ELISA kits (ng/mL)

Find out more about Instant ELISA kits at

thermoﬁsher.com/elisainstantkits

Parallelism of c-Myc (Total) ELISA

Antibody pair kits

Coat it yourself

Phosphospeciﬁc ELISA kits

Cell signaling assay

For studies of intracellular proteins involved in signaling, Invitrogen™ phosphospeciﬁc ELISA kits are available for measurement of total, and phosphorylated, modiﬁed, or cleavage site–speciﬁc proteins. Advantages and beneﬁts of these kits include:

• Speciﬁcity—two antibodies directed against the analyte provide better speciﬁcity than western blotting

• Sensitivity—more sensitive than western blotting

• Quantitation—get quantitative data in contrast to western blots (Figure 4)

• Medium throughput—96-well format, results in 4 hours, no densitometry analysis needed

Find out more about our phosphospeciﬁc ELISA kits at

thermoﬁsher.com/phosphoelisas

Optimized reagents–all in one box

Keep costs low

Uncoated ELISA kits are ideal for experienced ELISA users on a budget, who have less stringent requirements for inter- and intra-assay variance (Figure 5). Aordable ELISA kits using coat-it-yourself plates also include the necessary reagents to prepare and run an ELISA, including ELISA-optimized matched antibody pairs, standards, detection reagents, wash buers, and coating buers (plates are optional).

Beneﬁts of antibody pair kits

• Flexible—purchase based on the volume of your assay through a choice of package sizes, including 2-, 10-, and 20-plate kits

• Complete and easy to use—includes optimized antibody pairs and recombinant standards, TMB substrate, and all essential reagents and buers—unlike ELISA kits from other suppliers

O.D. 450 nm

Concentration (pg/mL)

c-Myc (57 kDa)

ELISA: O.D. 450

HeLa lysate (µg/test)

Figure 4. Conﬁrmation of western blotting data with quantitative results by phosphospeciﬁc ELISA assay. (A) Quantitative sample

parallelism data obtained using the Invitrogen Phospho-ELISA Kit (Cat. No. KHO2041). (B) Western blotting results using an Invitrogen in parallel.

™

Detection of c-Myc by ELISA vs. western blot

™

c-Myc (Total) Human

NuPAGE™ gel (Cat. No. NP0321). Assays were performed

Standard curve

HeLa lysate

• Aordable—priced to accommodate the most demanding budgets and to help maximize your research

3.50

3.00

2.50

2.00

1.50

1.00

Absorption (450 nm)

0.50

0.00

0.0 2.0

Figure 5. Representative standard curve for Invitrogen™ Mouse TGFbeta1 Antibody Pair Kit (Cat. No. 88-50690).

4.0 6.0 8.0 10.0 12.0 14.0

Concentration (ng/mL)

Using antibody pairs to make an ELISA

Watch our video at thermoﬁsher.com/ immunoassayvideos, then visit thermoﬁsher.com/ antibodypairs to discover what prematched antibody pairs

we oer to help make ELISA analysis easier.

Find out more about uncoated ELISA kits at

thermoﬁsher.com/uncoatedelisa

Accessory reagents and equipment

Thermo Fisher Scientiﬁc oers a comprehensive line of accessory reagents and equipment to support your immunoassay needs. Reagents include:

• Primary antibodies

• Secondary antibody conjugates

• Coated microplates

• Blocking buers

• Diluents

• Substrates

See a complete listing of accessory reagents for ELISA at

thermoﬁsher.com/elisaaccessories

Accessory equipment includes:

• Thermo Scientiﬁc™ Multidrop™ reagent dispensers

• Centrifuges

• Cold storage

• Single-channel pipettes

Microplate washers

Wellwash Microplate Washer

Wash 96-well plates securely with the Thermo Scientiﬁc™ Wellwash™ Microplate Washer, an easy-to-use, reliable microplate-strip washer for routine ELISA applications. The Wellwash system features a graphical user interface, local language versions, and USB port for optimal ease of use and convenience.

Wellwash Versa Microplate Washer

Wash 96- or 384-well plates securely with the Thermo Scientiﬁc™ Wellwash™ Versa Microplate Washer, an easy-to-use microplatestrip washer for routine ELISA applications. Designed for high performance and versatility, enjoy the ease of use and convenience provided through a graphical interface, local language version, and USB port.

See more at thermoﬁsher.com/wellwash

• Multichannel pipettes

• Pipette tips

• Plate washers

• Plate readers and analysis software

Get a comprehensive overview of immunoassay equipment oerings at

thermoﬁsher.com/elisainstrumentsandequipment

Considerations Wellwash washer Wellwash Versa washer

Languages 9 9

Plate types 96-well 96- and 384-well

Wash heads (depending on model)

Optional wash heads

Wash bottles One 2 L Two 2 L

Rinse bottle No One 2 L

Waste bottle One 2 L One 4 L

USB port for protocol import and export

Aerosol cover Yes Yes

Robot compatibility

IQ/QQ/PQ Yes Yes

1 x 8 or 1 x 12 Two 1 x 8 or two 1 x 12

Optional two 1 x 12 or two 1 x 8

1 x 12 or 1 x 8

Yes Ye s

No Ye s

Optional 1 x 16 (for 384-well plate)

Optional 2 x 8 (for 96-well plate)

Microplate readers Help reduce complexity and save time

Thermo Scientiﬁc™ Multiskan™ Sky Microplate Spectrophotometer is a next-generation UV/Vis microplate reader designed to be exceptionally convenient and easy to use for virtually any photometric research application, especially protein analysis.

• Ready-to-use built-in protocols allow fast and accurate quantitation of nucleic acids and proteins using the Thermo Scientiﬁc™ μDrop™ Plate, microplates, or cuvettes

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientiﬁc™ Varioskan™ LUX multimode microplate reader provides a ﬂexible range of measurement modes. The instrument simpliﬁes measurement setup with automatic dynamic range selection; its smart safety controls ease your workﬂow to help you avoid experimental errors. The Varioskan LUX multimode reader raises the bar for reliability and ease. The Varioskan LUX reader oers you:

• Versatility to run assays for photometric and ﬂuorometric quantitation of proteins and RNA

• Attractive user interface is optimized for touchscreen use, allowing quick setup of endpoint, kinetic, or

• Five measurement modes: endpoint, kinetic, spectral, multipoint, and kinetic spectra

spectral assays

• Spectral scanning for assay optimization

• Cloud-based tools such as Connect allow you to securely store, access, share, and manage your photometric data remotely

• Proven optical design enables top performance for a wide range of volumes and sample formats

• Modular, upgradable system for customization

Thermo Scientiﬁc™ SkanIt™ Software provides unlimited licenses with instrument purchase and ready-made sessions, available for running assays immediately. It is the same intuitive software that drives all

Find out more about our microplate readers at

thermoﬁsher.com/platereaders

Considerations Multiskan Sky spectrophotometer Varioskan LUX reader

Wavelength range (nm)

Wavelength selection

Wavelength scanning

Plate format 6- to 384-well

Incubation Ye s Yes Shaking Ye s Yes Dispensers No One or two (optional)

Top or bottom reading

Cuvette s Option No µDrop Plates Yes Yes Gas control module No Yes (optional) Onboard software Yes No PC software SkanIt Software (unlimited licenses) SkanIt Software (unlimited licenses) Robot compatibility Yes Yes

Applications

200–1,000

Monochromator

Yes Yes

NA Top or bottom (optional)

Immunoassays, including ELISA; protein assays (direct quantitation and colorimetric assays); nucleic acids (DNA, RNA) quantitation; cell proliferation, viability, and cytotoxicity assays; enzymatic activity assays; spectral and kinetic assays; endotoxin assays; bacterial grow th assays

Thermo Scientiﬁc™ microplate readers. Find out more at thermoﬁsher.com/skanit.

Fluorescence excitation and absorbance: 200–1,000 nm Fluorescence emission: 370–840 nm Luminescence: 360–670 nm TRF excitation: ﬁxed to 334 nm (spectral scanning 200 –840 nm) TRF emission: 400–700 nm (spectral scanning 270–840 nm) AlphaScreen excitation: ﬁxed to 680 nm AlphaScreen emission: 400–660 nm Monochromator

Filters for luminescence, TRF, and AlphaScreen tools

6- to 1,536-well (ﬂuorometry, TRF, luminometry, AlphaScreen tool) 6-to 384-well (absorbance)

Immunoassays, including ELISA; protein assays (Invitrogen and colorimetric assays); nucleic acids (DNA, RNA), quantitation (Quant-iT and direct assays); cell proliferation, viability, and cytotoxicity assays; enzymatic activity assays; luciferase assays; calcium assays, FRET, BRET, TR-FRET, AlphaScreen™ and AlphaLISA™ assays; spectral and kinetic assays; live-cell assays with integrated gas control module; QuantiGene Singleplex assay

™

Qua nt-iT™ assay, direct quantitation,

ProQuantum high-sensitivity

immunoassay kits

New

technology

The ProQuantum high-sensitivity immunoassay is a platform innovation that provides researchers with an easy-to-run, high-performance assay with no proprietary instrument to purchase. By utilizing proximity-based ampliﬁcation technology, we have combined the analyte speciﬁcity of high-anity antibody–antigen binding with the signal detection capabilities of real-time PCR, to achieve a highly sensitive protein quantitation assay with a large dynamic range. With its ability to detect even lower levels of protein than traditional methods with very small sample consumption, you can get the most out of your precious or limited samples.

Here’s how it works

The ProQuantum immunoassays utilize a matched pair of target-speciﬁc antibodies each conjugated to a DNA oligonucleotide. During antibody– analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of a template strand for ampliﬁcation. This proximity-based technology leverages the sensitivity and large dynamic range of Applied Biosystems™ TaqMan® real-time PCR technology (Figure 6).

Find out more and select your assay at thermoﬁsher.com/proquantum

thermoﬁsher.com/pqreleasenotice

The beneﬁts of ProQuantum immunoassays include:

• High-sensitivity—detect low levels of proteins with greater sensitivity than traditional methods

• Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to help ensure they fall within the range (Figure 7)

• Small sample consumption—use 2–5 µL of sample (for example, 2 µL vs. 75 µL for triplicate wells with other methods)

• Fast, easy workﬂow—2 hr from sample to answer, no wash steps, and a single 1 hr incubation

• No proprietary instrument to purchase—runs on any real-time PCR instrument

• Includes intuitive analysis software—comprehensive data analysis and statistical groupwise comparison

3´

10,000

∆C

0.5

0.25

0.125

0.0625

0.5

0.25

0.125

5PL regression

Protein conc.

450

3´

3´ oligo

5´ oligo 5´

Target

A B

Target

5´

Fluorescence

Cycle number

Figure 6. How ProQuantum immunoassay works. (A) Antibody-conjugates binding to target (1 hr incubation). (B) Ligation and ampliﬁcation of signal (in qPCR instrument).

0.01

0.1

IFN

0.1

IL-1β, pg/mL

, pg/mL

100

1,000

10,000

0.5

0.25

0.125

0.5

0.25

0.125

0.0625

450

0.01

0.1

IL-8, pg/mL

IL-6, pg/mL

100

1,000

Figure 7. Superior dynamic range. Standard curves for Invitrogen™ ProQuantum™ High-Sensitivity Human IFNγ, IL-1β, IL-8, and IL-6 immunoassay kits (red) show larger dynamic range and improved separation at the low end of the curve for greater sensitivity than normal and ultrasensitive ELISAs (purple).

Data analysis using ProQuantum software

The intuitive Invitrogen

™

ProQuantum™ cloud-based or desktop software is available to support your data analysis. This step-by-step software helps you set up your assay, including standard curve dilution calculation, plate layout for 96 or 384 wells, and customized lab bench instructions (cloud-enabled version only) (Figure 8). Once the assay has been performed, simply upload your run ﬁles from the qPCR instrument (see qPCR options on page 37) to the ProQuantum software and analyze. Analysis features include standard curve qualiﬁcation, outlier detection, 4 or 5PL regression ﬁt, results for unknown samples in pg/mL, and groupwise statistics. Easily access and securely share results with colleagues anywhere, anytime with our cloud-based Connect platform. Access free ProQuantum cloud-enabled software at apps.thermoﬁsher.com/apps/proquantum. Desktop version is now available (Cat. No. A43594).

A B

C D

Figure 8. Assay setup and analysis using ProQuantum software. (A) Standard curve setup. (B) Plate setup. (C) Customized lab instructions. (D) Data and results.

Need a custom ProQuantum assay? Lot reservation? Special bulk packaging?

We provide custom target development services of your chosen protein targets and immunoassay platform, as well as lot reservations and custom packaging for bulk orders.

Find out more at thermoﬁsher.com/iaspecialrequests

Green beneﬁts

• Less waste, use of fewer

resources: 47–59% less

plastic waste generated, 230 mL less water used per assay

• Sustainable packaging:

84% less packaging material

Multianalyte quantitation : assays and platforms

Protein multiplex immunoassays

• Quantitate up to 65 analytes in only 25 µL of serum or

plasma, or 50 µL of cell culture supernatant

Gene expression analysis

• Measure up to 80 RNA transcripts in a single well

Luminex instruments

Platforms that ﬁt your multiplex research needs

The Luminex xMAP technology combines advanced ﬂuidics, optics, and digital signal processing with ﬂuorescently dyed microspheres to enable the quantitation of multiple nucleic acid or protein targets from a single sample. The QuantiGene Plex assays are available for nucleic acid quantitation, and ProcartaPlex multiplex immunoassays for measurement of protein expression.

High-throughput immunoassays and gene expression analysis—all from one supplier, on one instrument

As a partner of Luminex, Thermo Fisher Scientiﬁc has been providing Luminex platform users with a comprehensive oering of multiplex reagents for over 18 years. Luminex Corporation has been supplying instruments to the life science research market for almost 20 years, with over 14,000 units installed worldwide.

antibodies binding to dierent epitopes of one protein. Invitrogen™ ProcartaPlex™ Simplex kits can be easily combined to create your own panels, or we can mix and validate a panel for you.

QuantiGene Plex assays for RNA and DNA quantitation

The QuantiGene Plex assay is a higher-throughput solution for multiplex analyses of gene expression, allowing researchers to measure up to 80 genes in a single well. This assay is fast, easy, and PCR-free—no RNA puriﬁcation is needed. Additionally, custom-made panels can be designed, validated, and shipped typically within two to three weeks of a request.

Why use Luminex technology?

Proﬁle more biomarkers with less starting sample:

Quantitate up to 80 analytes simultaneously with the FLEXMAP 3D or Luminex 200 instrument, and up to 50 analytes with the MAGPIX instrument. All of our ProcartaPlex assays are compatible with as little as 25µL of plasma or serum, or 50 µL of cell culture supernatant.

High-throughput analysis: QuantiGene Plex and ProcartaPlex assays are available in 96-well and 384-well formats for high-throughput analysis.

ProcartaPlex multiplex immunoassays

ProcartaPlex multiplex immunoassays are bead-based assays for protein detection using the Luminex xMAP technology. ProcartaPlex assays are based on the principles of a sandwich ELISA, using two highly speciﬁc

Comparison of dierent multiplex assay platforms

Assays Analyte measured Plex level Targets available Custom designs

ProcartaPlex multiplex immunoassays

QuantiGene Plex RNA assays RNA 80 >17,000 Yes

Proteins

Proven technology on a trusted platform: Hundreds of peer-reviewed publications have cited ProcartaPlex and QuantiGene Plex assays, demonstrating their value to researchers.

65 >500 Yes

Luminex xMAP technology

Quick, cost-eective, and accurate multianalyte proﬁling system

The open-architecture of Luminex xMAP technology uses ﬂow cytometry, microspheres, lasers, digital signal processing, and traditional chemistry—combining proven technologies in a unique way.

Advantages

• Helps reduce costs and labor through multiplexing

• Smaller sample size requirements compared to single-

result assays

• Enables fast, reproducible results from favorable kinetics

of liquid bead array approach

• Broad coverage of applications, including protein

expression and gene expression proﬁling

How Luminex xMAP technology works

Sets of magnetic microspheres are color-coded with combinations of red and infrared ﬂuorophores. Each bead set is coated with a reagent speciﬁc to a particular bioassay, allowing the capture and detection of several speciﬁc analytes from a single sample. Lasers in the compact analyzer excite the internal dyes that identify each microsphere as well as any reporter dye captured, providing quantitation of individual analytes on each microsphere. The analyzer reads many beads from each set, enabling rapid and precise results for several targets within a single sample.

Luminex xMAP technology

ProcartaPlex assays have adopted the Luminex xMAP magnetic bead technology that uses Luminex® MagPlex® superparamagnetic 6.5-micron microsphere beads with a magnetic core. The beads are internally dyed with precise proportions of red and infrared ﬂuorophores. Varying proportions of the red and infrared ﬂuorophores result in 100 spectrally unique microspheres, which are identiﬁed by the Luminex xMAP detection systems. The conjugation of a distinct monoclonal antibody to a distinct bead allows for analysis of multiple analytes in a single well.

6.5 microns

Bead set 26

Bead set 21

10 unique infrared dye concentrations

10 unique red dye concentrations

The bead is impregnated with the dye mixture

Find out which instrument is best for you and request a quote at thermoﬁsher.com/luminexinstruments

Luminex instrumentation

Luminex instrumentation consists of three instrument models: MAGPIX, Luminex 100/200, and FLEXMAP 3D systems. These instruments are based on either the principles of quantitative ﬂuorescence microscopy or ﬂuorescence ﬂow cytometry.

Accessories for Luminex technology

Description Cat. No.

xMAP Sheath Fluid (1X), 20 L 40500 15

xMAP Sheath Fluid Concentrate (20X), 1 L 40 50018

xMAP Classiﬁcation Calibrator Microspheres (CAL1)* L10 0 CAL1

xMAP Reporter Calibrator Microspheres (CAL2)* L100CA L2

xMAP Classiﬁcation Control Micropheres (CON1)* L100 CON1

xMAP Reporter Control Microspheres (CON2)* L100 CON 2

MAGPIX Performance Veriﬁcation Kit (25 reactions)

MAGPIX Calibration Kit (25 reactions) MPXCALK25

MAGPIX Drive Fluid (4-pack) MPXDF4PK

Luminex 200 Performance Veriﬁcation Kit LX2RPVERK25

Luminex 200 Calibration Kit LX2RCALK25

FLEXMAP 3D Performance Veriﬁcation Kit (25 reactions)

FLEXMAP 3D Calibration Kit (25 reactions) F3DCALK25

Hand-Held Magnetic Plate Washer EPX-55555-000

* 5 mL.

MPXPVERK25

F3DPVERK25

Aordable, ecient,

and compact in size

Versatile and the

standard in multiplexing

High-throughput,

high-plex

Feature MAGPIX Luminex 100/200 FLEXMAP 3D

Optics LED/CCD camera Lasers/APDs/PMTs Lasers/APDs/PMTs

Hardware Fluorescence imager Flow cytometry–based Flow cytometry–based

Multiplex capacity 50 100 500

Read time ~60 min/96-well plate ~30 min/96-well plate ~75 min/384-well plate

Applications Protein/nucleic acid Protein/nucleic acid Protein/nucleic acid

Dynamic range 3.5 logarithmic units 3.5 logarithmic units 4.5 logarithmic units

Microplate 96-well 96-well 96-well and 384-well

Footprint including PC (Linear bench space)

64.8 cm (24 in.) 80.0 cm (32 in.) 64.8 cm (24 in.)

Cat. No. APX1042 APX10 031 APX1342

Place a request for a quote from your local sales representative and ﬁnd out more at thermoﬁsher.com/luminexinstruments

ProcartaPlex multiplex immunoassay kits

Proﬁle more biomarkers with less sample

ProcartaPlex multiplex immunoassays use the Luminex xMAP (multianalyte proﬁling) technology that enables the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily ﬂuids. The technology employs the use of dierentially dyed, capture beads for each target in a multiplex “ELISA-like” assay. ProcartaPlex immunoassays can proﬁle up to 65 times more analytes using signiﬁcantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex immunoassays provide the ability to create your unique panel and enable reproducible results throughout the course of your study.

• More than 90% of ProcartaPlex assays can be combined with one another

• All ProcartaPlex assays are individually tested for speciﬁcity and endogenous, native protein detection

• Multianalyte detection with a large dynamic range

• Scalable and reproducible performance regardless of plex size

• Largest multiplex panel on the market for quantitation of up to 65 analytes

ProcartaPlex multiplex immunoassays are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine) to meet the needs of your research.

Get information regarding characteristic assay details and individual analytes at thermoﬁsher.com/procartaplex

ProcartaPlex multiplex assay options

Name Description

Predeﬁned, biologically relevant, and disease-deﬁned panels using

™

ProcartaPlex Preconﬁgured panels

ProcartaPlex Mix & Match panels

ProcartaPlex Simplex sets

magnetic beads for the quantitative multiplex analysis of up to 65 analytes in a single sample. Optimal performance and reproducibility through extensive testing for combinability, cross-reactivity and interference.

Custom-blended and optimized panels deliver results tailored to the panel

™

design of your choice. Simply provide your desired species, sample type, and instrument for use. Then select your desired analytes, and we will build and optimize a custom assay kit according to your speciﬁcations.

Bead sets for the detection of individual analytes designed to be added to ProcartaPlex panels for increased customization. Alternatively, multiple

™

ProcartaPlex simplex bead sets can be combined and run using the Invitrogen™ ProcartaPlex™ Basic Kit, which includes all non–target-speciﬁc reagents needed to perform the assay.

Mixing

required

No Magnetic Yes

Yes Magnetic No

Bead

type

Base kit

included

Have technical questions? Need help getting started? Email luminexfas@thermoﬁsher.com to get a one-on-one technical consultation.

Why ProcartaPlex assays?

Flexibility

Get exactly the panel you want. Since more than 90% of ProcartaPlex assays can be combined with one another, ProcartaPlex assays allow the creation of highly speciﬁc as well as high-plex panels. We currently oer the largest published panel with a 65-plex assay [1–4].

Reproducible results

ProcartaPlex assays are based on the principles of sandwich ELISA, where two highly speciﬁc antibodies recognize dierent epitopes on the same protein. Optimal performance and reproducibility are achieved through extensive multianalyte validation and optimization, enabling the delivery of consistent, reproducible, and high-quality assays.

Human IFN gamma ProcartaPlex/

3,500

R² = 0.9733

3,000

2,500

2,000

1,50 0

1,000

ProcartaPlex assay (pg/mL)

500

0 500 1,000 1,5 00 2,000 2,50 0 3,000

coated ELISA correlation

Coated ELISA (pg/mL) Coated ELISA (pg/mL)

Correlation to ELISA

Switch easily from ProcartaPlex assays to ELISA and vice versa with reliable results. As shown in Figure 9, equivalent results (R2 > 0.9) were obtained using the ProcartaPlex multiplex immunoassays and traditional plate-based ELISAs for human and mouse IFN gamma. ProcartaPlex assays achieve quantitative results comparable to traditional ELISA results with the added beneﬁts of reduced sample volume, shorter assay time, and lower cost.

Human TNF alpha ProcartaPlex/

450.0

R² = 0.96576

400.0

350.0

300.0

250.0

200.0

150.0

100.0

ProcartaPlex assay (pg/mL)

50.0

0.0

0.0 50.0 100.0 150.0 2 00.0 250.0 30 0.0 350.0 40 0.0 450.0

coated ELISA correlation

Mouse IFN gamma ProcartaPlex/

1,80 0

R² = 0.98201

1,60 0

1,40 0

1,20 0

1,000

800

600

400

ProcartaPlex assay (pg/mL)

200

0 500 1,000 1,50 0 2,000 2,500

coated ELISA correlation

7,000

R² = 0.99311

6,000

5,000

4,000

3,000

2,000

ProcartaPlex assay (pg/mL)

1,000

0.0

0.0 50.0 100.0 150.0 250.0 300.0 350.0 400.0 450.0

Mouse TNF alpha ProcartaPlex/

coated ELISA correlation

Coated ELISA (pg/mL) Coated ELISA (pg/mL)

Figure 9. Results of an experiment measuring IFN gamma and TNF alpha in stimulated human and mouse peripheral blood mononuclear cells (PBMCs). Supernatants were serially diluted two-fold in normal human or mouse serum, to allow for dierential cytokine levels in a serum matrix.

Additional cytokines for human and mouse analytes were compared, including IL-1 beta and IL-17A, and similar performance was obtained (data not shown).

Scalability

The ability to scale the number of analytes investigated in multiplex experiments is critical for the progression of many projects. For example, it is not uncommon to begin a research project by analyzing a large, comprehensive 35-plex cytokine panel on fewer samples (e.g., 100) to determine which analytes are aected by a particular disease or drug treatment. The resulting list of analytes can then be investigated further, for instance, using an 11-plex panel, on a larger set of samples. Thus, there is a great need for scalability in multiplex panel assays in order to

enable researchers to correlate all of their data across the dierent stages of their investigation. Many commercially available multiplex assays have not been developed with this level of consistency in mind. However, this speciﬁcation is a key design requirement incorporated during the ProcartaPlex assay development. Data in Figures 10 and 11 illustrate the high correlation between similar analytes in a large and small panel and the scalability when using ProcartaPlex assays. Similar results are obtained when investigating other analytes.

5,000

4,500

4,000

3,500

3,000

2,500

2,000

1,50 0

1,000

500

0.0

ProcartaPlex Th1/Th2 11-plex assay (pg/mL)

0.0 2,000 4,000 6,000

Procar taPlex /coated ELISA correlation

R² = 0.9995

ProcartaPlex 26-plex assay (pg /mL)

Figure 10. Data illustrates the high correlation between large- and small-scale multiplex scaling when using ProcartaPlex assays.

Regression analysis comparing the Invitrogen Cytokine & Chemokine Panel 1 (26-plex) and the ProcartaPlex Th1/Th2 Cytokine Panel (11-plex) revealed an R

™

ProcartaPlex™ Mouse

value >0.9995.

™

Mouse

Scalability with consistency

14.0

12.0

10.0

8.0

6.0

4.0

2.0

0.0

Serum sample 1

Serum sample 2

Serum sample 3

Serum sample 4

Serum sample 5

Serum sample 6

Serum sample 7

Serum sample 8

Serum sample 9

Serum sample 10

Serum sample 11

Serum sample 12

Serum sample 13

Serum sample 14

Serum sample 15

Serum sample 16

Serum sample 17

Serum sample 18

IL-6 34- ple x IL-6 Th1/T h2 11-plex

Figure 11. Scalability of ProcartaPlex multiplex immunoassays.

Human serum samples were run in parallel using both the Invitrogen ProcartaPlex

™

Human Cytokine & Chemokine Panel 1A (34-plex) and

™

the Human Th1/Th2 Cytokine Panel (11-plex). Experiments demonstrate consistency achieved in both large- and small-scale multiplex panels.

ProcartaPlex panels

ProcartaPlex human panel for immune monitoring

Immune Monitoring 65-Plex Human ProcartaPlex Panel

Cat. No. EPX650-10065-901

Analyte (bead region)

APRIL (88) GM-CSF (44) IL-7 (26 ) I L- 2 3 ( 6 3) MI P-1α (12)

BAFF (86) Gro α (61) I L-8 ( 2 7 ) IL-27 (14) MIP-1β (47)

BLC (29) HGF (46) I L-9 ( 5 2 ) IL-31 (37) MIP-3α (56)

βNGF (55) IFN α (48) I L-10 ( 2 8 ) IP-10 ( 22) MMP-1 ( 64)

CD30 (84) IFN γ (43) IL-1 2 p 7 0 ( 34) I-TAC (57 ) SCF (39)

CD40L (74) I L-1α ( 62 ) IL-13 (35) LIF (15) SDF-1α (13 )

ENA-78 (82) IL-1 β ( 1 8 ) IL-15 (65) M CP-1 ( 51) TNF-α (45)

Eotaxin (33) IL- 2 (1 9 ) I L-16 (7 0 ) MCP-2 (8) TNF-β (54)

Eotaxin-2 (30) I L- 2R ( 9 ) I L-17A ( 3 6 ) MCP-3 (68) TNF-R2 (85)

Eotaxin-3 (49) IL-3 (73) I L-18 (6 6 ) M-CSF (67) TRAIL (58)

FGF-2 (75) I L-4 (20 ) IL-2 0 ( 81 ) MDC (87) TSLP (80)

Fractalkine (59) I L- 5 (2 1) I L- 2 1 (72 ) MIF (53) TWEAK (97)

G-CSF (42) I L- 6 ( 25) IL-22 (76) MIG (69) VEGF-A (78)

A version of the Invitrogen™ Immune Monitoring

™

ProcartaPlex

Panel for MAGPIX system users is available. Cat. No. EPX650-16500-901

ProcartaPlex human panels ProcartaPlex human panel combinations

Th1/Th2 Cytokine 11-Plex Human ProcartaPlex Human Panel A

Cat. No. EPX110-10 810 -901

GM-CSF I L- 2 I L- 6 I L-18

IFN-gamma I L- 4 I L- 12 p70 TNF-alpha

I L-1 b e ta I L- 5 I L-13

Th9/Th17/Th22/Treg Cytokine 7-Plex Human

ProcartaPlex Panel

Cat. No. EPX070-10 817-9 0 1

I L- 9 I L-17A I L-2 2 IL-27

I L-10 I L-2 1 IL-23

Cytokine 7-Plex Human ProcartaPlex Panel 1C C

Cat. No. EPX070-10 010 - 90 1

IFN-alpha IL-1 R A I L-15 TNF-beta (LTA)

IL-1 alpha I L- 7 IL-31

Chemokine 9-Plex Human ProcartaPlex Panel 1 D

Cat. No. EPX090-12187-901

Eotaxin (CCL11) MIP-1 alpha (CCL3)

GRO-alpha (CXCL1) MIP-1 beta (CCL4)

IL-8 (CXCL8) SDF-1 alpha (CXCL12)

IP-10 (CXCL10) RANTES (CCL5)

MCP-1 (CCL2)

Growth Factor 11-Plex Human Procar taPlex Panel E

Cat. No. EPX110-12170-901

BDNF HGF SCF

NGF-beta LIF VEGF-A

EGF PDGF-BB VEGF-D

FGF-2 PlGF

Th9/Th17/Th22/Treg & Cytokine 14-Plex Human ProcartaPlex Panel 1C

Cat. No. EPX14 0 -12174 - 9 01

Cytokine 1C & Chemokine 16-Plex Human ProcartaPlex Panel 1

Cat. No. EPX160 -12176- 90 1

Th9/Th17/Th22/Treg & Chemokine 16-Plex Human ProcartaPlex Panel 1

Cat. No. EPX160 -12175 -90 1

Th1/Th2 & Cytokine 18-Plex Human ProcartaPlex Panel 1C

Cat. No. EPX180 -1217 2-9 0 1

Th1/Th2/Th9/Th17/Th22/Treg Cytokine 18-Plex Human ProcartaPlex Panel

Cat. No. EPX180 -1216 5-9 01

Th1/Th2 & Chemokine 20-Plex Human ProcartaPlex Panel 1

Cat. No. EPX 200-12173 - 9 01

Cytokine 25-Plex Human ProcartaPlex Panel 1B

Cat. No. EPX250 -1216 6-9 01

Cytokine & Chemokine 34-Plex Human ProcartaPlex Panel 1A

Cat. No. EPX340-12167-901

Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex Panel 1

Cat. No. EPX450-12171-901

B C

B D

A C

A B

A D

A B C

A B C D

A B C D E

C D

Immuno-oncology

The human immune system has evolved to protect the integrity of multicellular organisms against external threats such as malignant cells, which are altered due to carcinogenesis. Many advanced anticancer therapies aim to engage the power of the immune system to combat cancer. The goal of cancer immunotherapy is to initiate a self-sustaining cycle of cancer immunity, overcome negative feedback mechanisms, and avoid immunosuppressive molecules.

T cells and natural killer (NK) cells are important players in ﬁghting malignant cells, and soluble forms of receptors and ligands can be measured with Invitrogen™ ImmunoOncology Checkpoint ProcartaPlex™ Panels.

ProcartaPlex human panels for immuno-oncology

Immuno-Oncology Checkpoint 14-Plex Human

BTLA LAG-3 TIM-3 CD27

GITR PD1 CD28 CD152 (CTLA4)

HVEM PD-L1 CD80

IDO PD-L2 4-1BB (CD137)

Immuno-Oncology Checkpoint 14-Plex Human

MICA Perforin ULBP-1 Arginase-1 MICB PVR ULBP-3 ULBP-4

Nectin-2 Siglec-7 Ta c til e

NT5E Siglec-9 E-Cadherin

Immuno-Oncology Checkpoint 10-Plex Human

ICOS Ligand

(B7- H 2)

CD47 (IAP) CD48 (BLAST-1) Galectin-9 TIMD-4

CD134 (OX40) S100A8/A9

ProcartaPlex Panel 1

Cat. No. EP X14 A-15803 - 901

ProcartaPlex Panel 2

Cat. No. EPX140 -15 815 - 901

ProcartaPlex Panel 3

Cat. No. EPX100-15820-901

CD276 (B7-H3) VISTA (B7-H5) B7- H 6

CD28 PD-L2 CD152/CTLA-4 CD27 CD80 CD137/4-1BB I L- 2 I L-1 2 HVEM GlTR

Presentation of

cancer cell a ntigens

TNF-alpha I L-1 IFN-alpha

Priming and

activation

I L-1 0 I L- 4 I L-1 3

Release of can cer

cell antigens

Trafﬁcking of

T cells to tumors

Fractalkine IP-10 Rantes

Immune

checkpoint

inhibitors are

Inﬁltration of

T cells into tumors

revolutionizing cancer therapy

ICA M -1 VEGF

Killing of

cancer cells

PD - L1 PD -1 B7-1

Selectins

Recognition of

cancer cell s by T cells

IDO

TIM-3

BTLA

MIC

LAG-3

TGF-beta

For Invitrogen™ Immuno-Oncology Checkpoint Mouse panels, see page 26.

ProcartaPlex panels for human cytokine/chemokine/ growth factors

Essential Th1/Th2 Cytokine 6-Plex Human ProcartaPlex Panel

Cat. No. EPX060-10009-901

IFN-gamma I L- 5 I L-12 p 7 0

I L- 4 I L- 6 TNF-alpha

Essential 4-Plex Human Procar taPlex Panel 2

Cat. No. EPX040-10008-901

I L-10 be t a IL-8 (CXCL8) I L-10 IL-17A (CTLA-8)

Inﬂammation 20-Plex Human ProcartaPlex Panel

Cat. No. EPX200-12185-901

E-selectin IL-1 alpha I L-10 MCP-1 (CCL2)

GM-CSF I L-1 b et a IL-12p7 0

ICA M -1 I L- 4 I L-13

IFN-alpha I L- 6 IL-17A (CTLA-8) P-selectin

IFN-gamma IL-8 (CXCL8) IP-10 (CXCL10) TNF-alpha

Myokine 8-Plex Human ProcartaPlex Panel

Cat. No. EPX080-12186-901

BDNF I L- 6 I L-10 LIF I L-1R A IL-8 (CXCL8) I L-15 TNF-alpha

Chemokine 9-Plex Human ProcartaPlex Panel 2

Cat. No. EPX090-15840-901

CCL1 (I-309)

CCL25 (TECK)

CXCL6 (GCP-2)

MCP-4 (CCL13) CCL17 (TARC)

MIP-2 alpha

(CXCL2)

CCL21

(6Ckine/SLC)

MIP -1 beta

(CCL4)

MIP-1 alpha

(CCL3)

MIP-3 beta

(CCL19)

CCL23 (MPIF)

ProcartaPlex human panel for antibody isotyping

Antibody Isotyping 7-Plex Human ProcartaPlex Panel*

Cat. No. EPX070-10818- 901

IgG1 IgG2 IgG3 IgG4

IgA IgE IgM

ProcartaPlex panels for human cardiac proteins

Cardiac Disease 9-Plex Human ProcartaPlex Panel

Cat. No. EPX090-15809-901

Big E T-1 FABP-3 LDH-B NT proBNP

CKMB GDF -15 Myoglobin TNI

CT F -1

Atherosclerosis 9-Plex Human ProcartaPlex Panel

Cat. No. EPX090-15822-901

FGF-2 I L-8 MPO Tenascin-C

HSP60 LOX-1 PAI-1 TRAIL

I L- 6

Atherosclerosis 4-Plex Human ProcartaPlex Panel 2

Cat. No. EPX040-15824-901

Adiponectin CHI3L1/YKL-40 ICAM -1 Lp-PLA2

Cardiac Remodeling 8-Plex Human ProcartaPlex Panel

Cat. No. EPX080-15819-901

Gal-3 IL-33R LAP PTX3

IL-33 IP-10 OPN TREM-1

Arteriosclerosis 5-Plex Human ProcartaPlex Panel

Cat. No. EPX050-15821-901

BMP-2 FGF-23 OPG RANKL

COMP

ProcartaPlex human panel for graft versus host disease

Graf t Versus Host Disease 8-Plex Human ProcartaPlex Panel

Cat. No. EPX080-15814-901

CD30 HGF KRT18 Syndecan-1

Granzyme A I L- 2R ( C D 25 ) ST2/IL33R TNF-RI

ProcartaPlex human panels for metabolism/obesity

Metabolism/Obesity 5-Plex Human ProcartaPlex Panel 1*

Cat. No. EPX050-15805-901

RBP4 I C AM-1 NGAL Adiponectin

ZAG

Metabolism/Obesity 9-Plex Human ProcartaPlex Panel 2*

Cat. No. EPX09A-15804-901

C-peptide Cortisol FGF-23 GLP-1

Ghrelin Insulin PYY Leptin

Resisitin

Apolipoprotein 5-Plex Human ProcartaPlex Panel

Cat. No. EPX050-15818-901

APO A1 APO B APO E

APO A2 APO C2

* Panel cannot be combined with other ProcartaPlex panels or Simplex kits.

ProcartaPlex human panels for angiogenesis

Angiogenesis 18-Plex Human ProcartaPlex Panel

Cat. No. EPX180-15806-901

Angiopoietin-1 Follistatin Leptin TIE-2

BMP-9 G-CSF LY VE -1 VEGF-A

EGF HB-EGF PDGF-BB VEGF-D

EMMPRIN HGF PEC A M-1

FGF2 IL-8 Syndecan-1

Angiogenesis 3-Plex Human ProcartaPlex Panel 2

Cat. No. EPX030-15807-901

Angiogenin Angiostatin Endostatin

ProcartaPlex human panel for abundant serum markers

Abundant Serum Markers 26-Plex Human ProcartaPlex Panel

Cat. No. EPX260-15808-901

Adiponectin I CAM-1 NGAL Resistin

CD14 L-selectin N R P-1 SCGF-b

CD44 Lactoferrin Osteopontin TI M P-1

CD44var(var6) Leptin PA I -1 VCAM-1

Cystatin C MBL RANTES ZAG

Elaﬁn MMP-3 RBP4

Endoglin MMP-9 REG3a

ProcartaPlex panels for human coagulation

Coagulation 6-Plex Human ProcartaPlex Panel 1*

Cat. No. EPX060-10824-901

Factor XI Factor XII Factor XIII

Antithrombin Prothrombin CRP

Coagulation 3-Plex Human ProcartaPlex Panel 2*

Cat. No. EPX030-10823-901

Factor V Factor VII Factor VIII

Coagulation 4-Plex Human ProcartaPlex Panel 3*

Cat. No. EPX040-10825-901

Factor IX Protein S Protein C vWF

ProcartaPlex human panel for MMP

MMP 5-Plex Human ProcartaPlex Panel 1

Cat. No. EPX050-10015-901

MMP-1 MMP-8 MM P-12 MMP-13

MM P-7

ProcartaPlex human panels for TNFSF

TNF Superfamily 15-Plex Human ProcartaPlex Panel*

Cat. No. EPX150-15 801- 901

APRIL EDA -1 NGF-beta TRAIL

BAFF FasL RANKL Tweak

CD30L GlTRL TNF-alpha

CD40L LIGHT TNF-beta

TNF Receptor Superfamily 12-Plex Human ProcartaPlex Panel*

Cat. No. EPX120-158 02- 901

CD30 DR6 TNF-R1 T R AI L- R 1

CD40 Fas (APO) TNF-R2 TRAIL-R2

GITR HVEM 4-1BB (CD137) CD27

4-1BBL

(CD137L)

Procarta Plex Panels for neurobiology

Neuroscience 18-Plex Human ProcartaPlex Panel

Cat. No. EP X18 0 -15837-901

Tau (Total)

YKL40/CHI3L1 NF-H KLK6 NCAM

FGF-21 TDP-43 GFAP UCHL

S100b GDNF CNTF BDNF

bNGF MIF

Neurodegeneration 9-Plex Human ProcartaPlex Panel 1

Tau (Total)

Neurogranin TDP-43 KLK6 NCAM

FGF-21

Neurodegeneration 4-Plex Human ProcartaPlex Panel 2

Apolipoprotein

Brain Injury 5-Plex Human ProcartaPlex Panel

NF-H GFAP UCHL S10 0b

NSE

Neurotrophic Factors 4-Plex Human ProcartaPlex Panel

GDNF CNTF BDNF bNGF

Neuroinﬂammation 6-Plex Human ProcartaPlex Panel

IL-34 CXCL13 (BLC) MIF Soluble RAGE

TREM-2 YKL40/CHI3L1

Tau (Phospho)

[T181]

Cat. No. EPX090-15836-901

Tau (Phospho)

[T181]

Cat. No. EPX040-15832-901

Clusterin (Apo-J)

Cat. No. EPX050-15838-901

Cat. No. EPX040-15828-901

Cat. No. EPX060-15833-901

Aβ 1-42 Neurogranin

Aβ 1-40 Aβ 1-42

Complement

Factor H

Fetuin-A

ProcartaPlex human panel for apoptosis

Apoptotic Cell Clearance 12-Plex Human ProcartaPlex Panel

Cat. No. EPX120 -15816-901

AXL Gas6 Mer (MERTK) RAGE

CALR (CRT) LOX-1 PAI-1 (Serpin) TYRO3

CD36 MBL PECA M -1 uPAR

* Panel cannot be combined with other ProcartaPlex panels or Simplex kits. For Research Use Only. Not for use in diagnostic procedures.

ProcartaPlex mouse panels

Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex Panel A

Cat. No. EPX110-20820-901

GM-CSF I L- 2 IL- 6 IL-1 8

IFN-gamma I L- 4 I L-12 p 7 0 TNF-alpha

I L-1 b e ta IL- 5 IL-1 3

Th9/Th17/Th22/Treg Cytokine 6-Plex Mouse Procar taPlex Panel B

Cat. No. EPX060-20822-901

I L- 9 I L- 17A I L- 2 3

I L-10 I L- 2 2 IL-27

Cytokine 10-Plex Mouse ProcartaPlex Panel 1B C

Cat. No. EPX100-26091-901

G-CSF (CSF-3) I L-2 8 IL-3 M-CSF (CSF-1)

IFN-alpha IL-31 LIF

I L-15 (I L-15 R) IL-1 alpha ENA-78 (CXCL5)

Chemokine 9-Plex Mouse ProcartaPlex Panel 1 D

Cat. No. EPX090-20821-901

Eotaxin (CCL11) MCP-1 (CCL2) MIP-1 beta (CCL4)

GRO-alpha (CXCL1) MCP-3 (CCL7) MIP-2 (CXCL2)

IP-10 (CXCL10) MIP-1 alpha (CCL3) RANTES (CCL5)

ProcartaPlex mouse panel combinations

Th9/Th17/ Th22/Treg & Chemokine 15-Plex Mouse ProcartaPlex Panel

Cat. No. EPX150-26089-901

Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex Panel

Cat. No. EPX170-26 0 87- 901

Th1/Th2 & Chemokine 20-Plex Mouse ProcartaPlex Panel 1

Cat. No. EPX200-26090-901

Cytokine & Chemokine 26-Plex Mouse ProcartaPlex Panel 1

Cat. No. EPX260-26088-901

Cytokine & Chemokine 36-Plex Mouse ProcartaPlex Panel 1A

Cat. No. EPX360-26092-901

B D

A B

A D

A B D

A B C D

ProcartaPlex mouse panels for immune monitoring

Immune Monitoring 48-Plex Mouse

GM-CSF I L-15/ I L-15 R I L- 2 R A LIF

BAFF I L-1 7A IL-3 MC P-1

BTC I L-18 IL-31 MCP-3

ENA-78//CXCL5 I L-19 IL-33 M-CSF

Eotaxin I L-1α I L- 4 MIP-1 alpha

G-CSF/CSF-3 I L-1ß I L-5 M I P-1 b e t a

GROα/KC/

CXCL1

IFN-alpha I L-2 2 I L-7 RANKL

IFN-gamma I L- 23 I L- 7 R α RANTES

I L-10 IL-25 / I L-17 E IL- 9 ST2/IL-33R

I L-12 p 7 0 IL-27 IP-10 TNF-α

I L-13 IL- 2 8 Leptin VEGF-A

Immuno-Oncology Checkpoint 4-Plex Mouse

BTLA CD27 LAG-3 TIM-3

Immuno-Oncology Checkpoint 7-Plex Mouse

CD137L

(4-1BBL)

PD -1 PD-L1 PD-L2

ProcartaPlex Panel

Cat. No. EPX480-20834-901

I L- 2 IL- 6 MIP-2

ProcartaPlex Panel 1

Cat. No. EPX040-20830-901

ProcartaPlex Panel 2

Cat. No. EPX070-20835 -901

CD276 (B7-H3) CD80 CTLA-4

(CD152)

ProcartaPlex mouse panels for antibody isotyping

Antibody Isotyping 7-Plex Mouse

ProcartaPlex Panel*

Cat. No. EPX070-20815-901

IgG1 IgM IgA IgE

IgG3 IgG2a IgG2b

Antibody Isotyping 7-Plex Mouse

ProcartaPlex Panel 2*

Cat. No. EPX070-20816-901

IgG1 IgM IgA IgE

IgG3 IgG2b IgG2c

* Panel cannot be combined with other ProcartaPlex panels or Simplex kits.

ProcartaPlex rat panels

Th Complete 14-Plex Rat ProcartaPlex Panel A

Cat. No. EPX140-30120-901

G-CSF (CSF-3) IL- 2 I L-12 p 7 0

GM-CSF IL- 4 I L-13

IFN-gamma I L-5 IL-17A (CTLA-8)

IL-1 alpha IL- 6 TNF-alpha

I L-1 b e ta IL-1 0

Chemokine 8-Plex Rat ProcartaPlex Panel B

Cat. No. EPX080-30121-901

Eotaxin (CCL11) MCP-1 (CCL2) MIP-2 (CXCL2)

GRO-alpha

(KC/CX CL1)

IP-10 (CXCL10) MIP-1 alpha (CCL3)

MCP-3 (CCL7) Rantes (CCL5)

ProcartaPlex rat panel combinations

Cytokine & Chemokine 22-Plex Rat ProcartaPlex Panel

Cat. No. EPX220-30122-901

A B

ProcartaPlex rat panels for antibody isotyping

Antibody Isotyping 6-Plex Rat ProcartaPlex Panel*

Cat. No. EPX060-30123-901

IgG1 IgG2a IgG2b

IgG2c IgA IgM

ProcartaPlex rat panels for kidney toxicity

Kidney Toxicity 5 -Plex Rat ProcartaPlex Panel 1*

Cat. No. EPX050-30124-901

KIM-1 VEGF-A TFF-3

Osteopontin Calbindin

Kidney Toxicity 5 -Plex Rat ProcartaPlex Panel 2*

Cat. No. EPX050-30125-901

Cystatin C T IMP-1 Clusterin

Albumin NGAL

ProcartaPlex nonhuman primate panels

Th 14-Plex NHP ProcartaPlex Panel A

Cat. No. EPX140-40040-901

GM-CSF IL- 5 I L-17A

IFN-gamma I L- 6 I L- 18

I L-1 b e ta IL-1 0 I L-23

I L- 2 IL-12p7 0 TNF-alpha

I L- 4 I L-13

Cytokine 6-Plex NHP ProcartaPlex Panel 1B B

Cat. No. EPX060-40042-901

CD40L IFN-alpha I L-15

G-CSF I L-1 R A IL-7

Chemokine 10-Plex NHP ProcartaPlex Panel C

Cat. No. EPX100-40041-901

BLC (CXC L13 ) I-TAC (CX C L11) MIP-1 beta (CCL4)

Eotaxin (CCL11) MCP-1 (CCL2) SDF-1 alpha (CXCL12)

IL-8 (CXCL8) MIG (CXCL9)

IP-10 (CXCL10) MIP-1 alpha (CCL3)

Growth Factor 7-Plex NHP ProcartaPlex Panel D

Cat. No. EPX070-40043-901

BDNF PDGF-BB VEGF-D

NGF-beta SCF

FGF-basic VEGF-A

ProcartaPlex nonhuman primate panel combinations

Cytokine & Chemokine 30-Plex NHP ProcartaPlex Panel

Cat. No. EPX300-40044-901

Cytokine/Chemokine/Growth Factor 37-Plex NHP ProcartaPlex Panel

Cat. No. EPX370-40045-901

A B C

A B C D

ProcartaPlex porcine panels

Cytokine & Chemokine 9-Plex Porcine ProcartaPlex Panel 1

Cat. No. EPX090-60829-901

IFN-alpha I L- 4 I L- 10

IFN-gamma I L- 6 IL-12 /IL- 2 3 p 40

I L-1 b e ta IL-8 (CXCL8) TNF-alpha

ProcartaPlex canine panels

Cytokine/Chemokine/Growth Factor 11-Plex Canine

IFN-gamma IL-8 (CXCL8) SCF

I L- 2 I L-10 TNF-alpha

I L- 4 IL-1 2 / IL- 23 p 4 0 VEGF-A

I L- 6 MCP-1 (CCL2)

ProcartaPlex Panel 1

Cat. No. EPX11A-50511- 9 0 1

* Panel cannot be combined with other ProcartaPlex panels or Simplex kits. For Research Use Only. Not for use in diagnostic procedures.

ProcartaPlex high-sensitivity

immunoassay kits

Invitrogen™ ProcartaPlex™ high-sensitivity immunoassay kits oer accurate quantitation of key cytokines signiﬁcantly below the detection limit of conventional Luminex assays.

ProcartaPlex high-sensitivity immunoassay kits feature

• Highest sensitivity detection of cytokine concentrations, in fg/mL

• Inter- and intra-assay CV <10%

• Small sample input (25 μL) ideal for precious serum or plasma samples

• Easy-to-use, simple ELISA-like protocol completed in less than 4 hr

• Convenient, ready-to-use kits for quantitation of up to 9 cytokines

• Trusted platform based on the principles of a sandwich ELISA and utilizing Luminex xMAP (multianalyte proﬁling) technology

The ProcartaPlex high-sensitivity immunoassays enable the multiplexed analysis of human and mouse analytes in very low concentrations in various matrices, including serum, plasma, bodily ﬂuids, and cell culture supernatant samples. These high-sensitivity multiplex assays are ideal when sample input is limited, and protein expression is in the low pg/mL range.

Accurate quantitation below conventional limits is achieved through a proprietary signal ampliﬁcation technology. The signal ampliﬁcation has negligible eects on the assay background, thereby increasing the signal-to-noise ratio of the cytokine standards on the lower end of the standard curve. This also allows for more accurate and reproducible measurement of cytokines with low expression levels.

ProcartaPlex high-sensitivity panels

High Sensitivit y 9-Plex Human ProcartaPlex Panel

Cat. No. EPXS090-12199-901

IFN-gamma I L- 4 I L-12 p70

I L-1 b e ta I L- 6 IL-17A (CTLA-8)

I L- 2 I L-10 TNF-alpha

High Sensitivit y 5-Plex Mouse ProcartaPlex Panel

Cat. No. EPXS050-22199-901

IFN-gamma

I L- 2 IL- 6

I L- 4 TNF-alpha

Assay speciﬁcations and data

Measure low-abundance cytokines with our ProcartaPlex high-sensitivity immunoassays. We oer simplex kits and panels for human and mouse cytokines. Find the most current oering of our available high-sensitivity assays at thermoﬁsher.com/highsensitivityprocarta.

ProcartaPlex Simplex human high-sensitivity kits

Cat. No. Analyte EPXS010-10283-901 GM-CSF 7, 33 0 1.8 <10 <10 EPXS010-10228 -901 IFN-γ 260 0.31 <10 <10 EPXS010-10224 -9 01 IL-1 β 1,3 30 0.32 <10 <10 EPXS010-10221- 90 1 IL- 2 490 0.44 <10 <10 EPXS010-10225 -901 I L- 4 560 0.86 <10 <10 EPXS010-10278 -9 01 I L- 5 2,720 0.66 <10 <10 EPXS010-10213-901 I L- 6 2,850 1.0 3 <10 <10 EPXS010-10204-901 IL-8 890 0.22 <10 <10 EPXS010-10215-901 I L-10 750 0 .18 <10 <10 EPXS010-10238-901 I L-1 2 p70 3,090 0.75 <10 <10 EPXS010-12017-901 IL- 17A 1,03 0 0.25 <10 <10 EPXS010-10281- 90 1 MC P-1 650 0.16 <10 <10

ProcartaPlex Simplex mouse high-sensitivity kits

Cat. No. Analyte

EPXS010-20606-901

EPXS010-20601-901 IL- 2 1,8 20 0.44 <10 <10 EPXS010-20613-901 IL-4 3,530 0.86 <10 <10 EPXS010-20603-901 I L- 6 4,230 1.03 <10 <10

EPXS010-20607-901

* Upper limit of quantitation. ** Lower limit of quantitation.

IFN-

gamma

TNF-

alpha

ULOQ*

(pg/mL)

ULOQ*

(pg/mL)

1,250 0.31 <10 <10

1,8 30 0.45 <10 <10

LLOQ**

(pg/mL)

LLOQ**

(pg/mL)

Inter-

assay CV

Inter-

assay CV

Intra-

assay CV

Intra-

assay CV

Immunoassay Special Services

Our immunoassay R&D team has over 30 years of experience with immunoassay development, sample handling, data analysis, and project management. Operating in an ISO 9001–certiﬁed facility, we employ high-quality standards and procedures.

• Not sure where to start? We oer valuable consultation services, free of charge.

• Can’t ﬁnd a commercially available assay for your target? We oer custom assay development.

• Short on sta resources? Tight deadlines? Or just want experienced scientists to run your samples and provide results to you? We oer sample testing services.

• Starting a large or long-term research project? We oer helpful services like special bulk packaging and lot reservation.

Custom immunoassay development and services

• Have us develop a custom assay unique to your target, with project milestones and progress reports

• Request custom plate coating to enable consistency across experiments (ELISA only)

• Request additional QC testing of assays

Special packaging

• Obtain products in bulk packaging for less waste, better use of storage space, or high-throughput automation needs

• Request additional components or special formulation

Sample testing services

• Send us your samples and we will run the assay for you

Lot reservation

• Request multiple kits from a single lot for large or longterm studies

Immunoassay support center

Assay development service

Custom assay development services are available for protein targets that are not commercially available. By using our services, you leverage years of assay development experience without wasting your time. Let us help save you time so you can work on the important parts of your research.

• Utilize technical specialists with over 30 years of assay development experience

• Save time and money, and avoid the hassle of optimizing and troubleshooting

• Be conﬁdent that you’re getting the best assay possible

Access the custom assay services quote request form at thermoﬁsher.com/immunoassayrequests

Sample testing service

No Luminex instrument available? Not sure if this is the right platform for you? Our service team comprises highly skilled and experienced professionals who will work with you throughout the stages of your project to help ensure delivery of high-quality data and results. This allows you to test the technology and see if the data meets your expectations.

• Be conﬁdent that your samples are being tested by experienced technical specialists

• Receive high-quality reports of your assay data

• Minimal investment needed to make a decision before investing in the Luminex platform

Find out more about sample testing services at

thermoﬁsher.com/iarequests

Find out more about QuantiGene assays at

thermoﬁsher.com/quantigeneassayservice

ProcartaPlex Mix and Match panels

Design your own multiplex panel

Build the panel of your choice with our online Invitrogen™ ProcartaPlex™ Panel Conﬁgurator. Design your own custom assay blend at thermoﬁsher.com/mixandmatchluminex.

ProcartaPlex analytes

Human

Mouse

4-1BB (CD137) •

4-1BB L (C D137L) •

6Ckine (CCL21) •

Albumin* •

Adiponectin • • •

Amyloid beta (1–40) •

Amyloid beta (1–42) •

Angiogenin •

Angiopoietin-1 •

Angiostatin •

Antithrombin* •

Apolipoprotein E4 •

APRIL •

Arginase-1 •

AXL •

B7- H6 •

BACE1 (beta-secretase 1) •

BAFF • •

BLC (CXCL13) • •

BDNF • •

Betacellulin (BTC) •

Bi g ET-1 •

BMP-2 •

BMP-9 •

BTL A (CD272) • •

C-peptide •

C3a •

Calcitonin •

CALR (CRT ) •

Caspase-3 (total) •

CD14 •

CD27 • •

CD28 •

CD30 •

CD30L •

CD36 •

CD40 •

CD40L • •

CD44 •

CD44var (6var) •

CD80 (B7-1) •

CD13 4 (O X40 ) •

CD137L (4 -1BBL ) •

CD276 (B7-H3) • •

CD47 (IA P) •

CD48 (BLAST-1) •

Rat

NHP

ProcartaPlex analytes

Canine

Porcine

CD80 •

CHI3L1/YKL-40 •

CKMB •

Clusterin* •

Clusterin (Apo-J) •

CNTF •

COMP •

Complement factor H •

Cortisol •

CRP • • •

CTACK (CCL27) •

CT F-1 •

CTLA-4 (CD152) •

Cysistin C* •

Cystatin C •

D-Dimer •

DR6 •

E-cadherin •

ED A-1 •

EGF •

Elaﬁn •

EMMPRIN •

ENA-78 (CXCL5) • •

Endoglin (CD105) •

Endostatin •

Eotaxin (CCL11) • • • •

Eotaxin-2 (C CL24) •

Eotaxin-3 (CCL26) •

E-selectin (CD62E) •

Factor V* •

Factor VII* •

Factor VIII* •

Factor IX* •

Factor X* •

Factor XI* •

Factor XII* •

Factor XIII* •

Fas (APO) •

Fas-L •

Ferritin •

Fetuin-A •

FGF-2 • •

FGF-21 •

FGF-23 •

Fibrinogen •

Flt3-ligand •

Human

Mouse

Rat

NHP

Canine

ProcartaPlex analytes

Porcine

Follistatin •

Fractalkine (CX3CL1) •

Galectin-3 •

Galectin-9 •

GAPDH •

GAS6 •

G-CSF (C SF-3) • • • •

GCP-2 (CXCL6) •

GD F-15 •

GDNF •

GFAP •

GITR (CD357) •

GITRL •

GM-CSF • • • •

gp130 (IL-6RB) •

Granzyme A •

Granzyme B • •

GRO alpha (KC/CXCL1) • • •

GL P-1 •

Ghrelin •

HB-EGF •

HER-2 •

HGF •

HSP60 •

HVEM (CD270) •

I-309 (CCL1) •

Insulin •

IC AM-1 • • •

ICOS ligand (B7-H2) •

IFN-alpha • • • •

IFN-beta • • •

IFN-gamma • • • • • •

IFN-omega • •

Ig A* • • •

IgE* • •

Ig G1* • • •

IgG2* •

IgG2a* • •

IgG2b* • •

IgG2c* • •

IgG3* • •

IgG4* •

IgM* • • •

IDO •

I L-1 a l p h a • • •

I L-1 b e t a • • • • •

Human

Mouse

Rat

NHP

Canine

Porcine

ProcartaPlex analytes

Human

Mouse

Rat

NHP

I L-1 R A • •

I L-2 • • • • •

I L-2 RA (C D2 5) • •

IL-3 • •

I L- 4 • • • • • •

I L- 5 • • • •

I L- 6 • • • • • •

I L-7 • • •

I L-7 RA (C D12 7 ) •

IL-8 (CXCL8) • • • •

I L- 9 • •

I L-1 0 • • • • • •

IL-12/IL-23p40 • • • • • •

I L-12 p 7 0 • • • •

I L-13 • • • •

I L-15 • •

I L-15/ IL- 15 R •

I L-16 •

I L-17 A (C TL A- 8) • • • •

I L-17 A F •

I L-17 F • • •

I L-18 • • •

I L-19 •

I L-2 0 •

I L-2 1 • • •

I L-2 2 • •

I L-2 3 • • •

I L-2 5 •

IL-27 • •

IL-29 (IFN-lambda 1) •

IL-31 • •

IL-33 • •

IL-34 •

IL-37 •

IP-10 (CXCL10) • • • •

I-TAC (CXCL11) • •

Kallikrein-6 (KLK6) •

KI M-1* •

KRT18 •

Lactoferrin •

LAG-3 • •

LAP ( TGF-beta 1) •

LDH-B •

Leptin • • •

LIGHT •

LIF • •

LOX-1 •

Lp-PLA2 •

L-selectin •

LY VE -1 •

MBL •

MCP-1 (CCL2) • • • • •

MCP-2 (CCL8) •

MCP-3 (CCL7) • • • •

MCP-4 (CCL13) •

M-CSF • •

MDC (CCL22) •

Mer (MERTK) •

Canine

ProcartaPlex analytes

Porcine

MICA •

MICB •

MIF • •

MIG (CXCL9) • •

MIG (CXCL9) •

MIP-1 alpha (CCL3) • • • •

MIP-1 beta (CCL4) • • •

MIP-2 alpha (CXCL2) • • •

MIP-3 alpha (CCL20) •

MIP-3 beta (CCL19) •

MM P-1 •

MMP-2 •

MMP-3 •

MMP-7 •

MMP-8 •

MMP-9 •

MM P-12 •

MM P-13 •

MPIF (CCL23) •

Myeloperoxidase (MPO) •

Myoglobin •

NC AM-1 •

Nectin-2 •

Neurogranin (NRGN) •

NF-H •

NGAL • •*

NGF-beta • • • •

NR P-1 •

NSE •

NTproBNP •

NT5E •

Oncostatin M (OSM) •

Osteopontin • •*

Osteoprotegerin (OPG) •

PAI-1 (Serpin) • •

PDGF-BB • •

PD -1 •

PD -1 ( CD279) •

PD -L1 •

PD-L1 (CD274) •

PD-L2 • •

PE CA M-1 •

Perforin •

PIGF •

Protein C* •

Protein S* •

Prothrombin* •

P-selectin •

PTX3 •

PVR •

PYY •

RAGE •

RBP4 •

RANKL • • •

RANTES (CCL5) • • • •

REG3a •

Resistin (ADSF) • •

S100 A8/A 9 •

Human

ProcartaPlex analytes

Mouse

Rat

NHP

Canine

Porcine

S100 B •

SAA •

SAP (Pentraxin 2) •

SCF • • •

SCGF-beta •

SDF-1 alpha (CXCL12a) • •

Siglec-7 •

Siglec-9 •

ST-2 •

ST2 (IL-33R) • •

Survivin (BIRC5) •

Syndecan-1 •

TARC (CCL17) •

Tac ti le •

Tau (To tal ) •

Tau [pT181] •

Tau [pT231] •

TDP-43 •

TECK (CCL25) •

Tenascin-C •

TFF-3* •

TGF-alpha • •

TGF-beta 1 • • • • • •

Thrombopoietin (TPO) •

TIE-2 •

TIM-3 (CD366) • •

TIMD-4 •

TI MP-1 • •*

TNF-alpha • • • • • •

TNF-beta • •

TNF-R1 •

TNF-R2 •

TNI •

tPA •

TRAIL • •

TRAIL-R1 •

TRAIL-R2 •

TREM-1 •

TREM-2 •

TSLP • •

Twe ak •

TYRO3 •

UCHL1 •

UL BP-1 •

ULBP-3 •

ULBP-4 •

uPAR •

VC AM -1 • • •

VEGF-A • • • • •

VEGF-D • •

VEGF-R1 •

VEGF-R2 •

VEGF-R3 •

VISTA (B7-H5) •

vWF* •

ZAG •

Human

Mouse

Rat

NHP

Canine

Porcine

* Analytes only available as part of a panel.

Multiplexed gene expression assays: QuantiGene Plex assays

Accurate and precise RNA quantitation of up to 80 gene transcripts in a single well (96- and 384-well format)

Overview of QuantiGene Plex assays

The QuantiGene Plex assays provide an accurate and precise method for multiplexed gene expression quantitation. Using the Luminex xMAP technology, QuantiGene Plex assays allow for simultaneous measurement of 3–80 RNA species in every well of a 96- or 384-well plate. Branched DNA assays allow for the direct measurement of RNA transcripts by using signal ampliﬁcation rather than template ampliﬁcation.

The QuantiGene Plex assays are simple and easy to use; they don’t require RNA extraction, cDNA synthesis, or PCR ampliﬁcation. QuantiGene Plex assays are ideal for verifying microarray or next-generation sequencing (NGS) data and for verifying biomarkers for translational research.

Key features and beneﬁts of the assay

• Works with dicult sample types—works with degraded and crosslinked RNA in formalin-ﬁxed, paran-embedded (FFPE) tissues and directly with blood, with no RNA puriﬁcation required

• True multiplexing—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity; reduces the number of sample wells and sample amount needed

• Standardized platform—96-well plate format compatible with Luminex 100/200, MAGPIX, and FLEXMAP 3D systems

• Simple workﬂow—ELISA-like workﬂow for direct hybridization of transcripts to beads and transcript labeling, and no reverse transcription needed

• Large inventory of veriﬁed genes—over 22,500 genes can be mixed in up to 80-plex panels to create pathway- and disease-themed panels

• Faster customization than other methods—if we don’t have your gene(s), we can create and QC your custom panel typically in 2–3 weeks

• ISH compatible—same technology utilized in the Invitrogen™ ViewRNA™ Kits for mRNA in situ hybridization (ISH)

Sample

preparation

Target

hybridization

Luminex beads

with ca pture pro bes

Signal

ampliﬁcation

Preampliﬁer

Detection

Streptavidin

phycoerythrin (SAPE)

Lyse sample

Lysa te (with ta rget RNA )

Incubate beads with sample and probes

Capture extend ers (CE )

Label extend ers (LE )

Blocking probes (BL)

Lysa te (with ta rget RNA )

Ampliﬁer

Label probe

Hybridizations and wash steps

Read signal using a Lumin ex instrument

80 RNA t argets

per wel l

QuantiGene Plex assay applications

Basal Luminal

HCC70

HCC3153

MDAMB468

SUM1314

BT20

H5578T

BT549

MDAMB157

MDAMB231

MDAM453

LY2

SUM185P E

T47D

HCC202

BT474

ZR751

MDAMB361

AU565

SKBR3

EN1 FOXC1 GABRP ERBB2 CA12 AGR2 TFF3 GATA3 ESR1 SCNN1

−1

−2

−3

−4

10–¹

–

–4

–5

p40 p35 TNF p19 EB13 p28

LPS

+ LPS

Fluorescence/µg RNA 2

–

∆∆CT

V669-2

Cloﬁbrate

Fold induction vs. vehicle control

Fold induction of mRNA

Rifampin

3-Methylcholanthrene Phenobarbital Ritonavir Omeprazole Phenytoin ß-Napthoﬂavone

mRNA CYP panel

1A1 1A2 1E1 2A6 2C6 2C9 2C18 2C19 2B6 2D6 2E1 3A4 3A5

6mm

6 mm

Liver FFPE sections

Section 1

Section 2

Mean uorescence intensity

FFPE liver sections—1 vs. 2

400

800

1,200

1,600

6 mm

H&E-stained

section 1

Unstained

section 2

–5

–4

–2

pc a2$ x[.1]

pca2$x [.2]

Gene P value

BCL6 1.28 x 10

–9

PTEN 1.03 x 10

–8

ARPC2 2.17 x 10

–8

CXCL12 1.37 x 10

–7

BRAF 4.18 x 10

–7

PCNA 6.33 x 10

–7

CLEC3B 1.13 x 10

–6

Metastatic melanoma

Normal PCA

Principal component analysis of 7 genes

M17

M17: Not melanoma; lymph node

with pigmented histiocytes

CD86 IL2RACCL8IL8CXCL5 Cig5CXCL10TNFalP6IFNGIL1R1 IFIT3

Ratio of CXCL10 -treated to untreated

CXCL10 s timulation of whole blood f or 6 hr and QuantiGene ana lysis of gene expression

0.3 µg CXCL10

1.0 µg CXCL10

1.0 µg CXCL10 Heat-inactivated

QuantiGene Plex assays for RNA proﬁling are ideal for supporting drug discovery and development eorts, as well as translational and clinical research. The examples below highlight the capabilities and beneﬁts of this powerful research tool.

veriﬁcation of cell-based microarrays

Patient-derived xenograft

Tumor cell line characterization

for drug compound screening

Application: Stimulation of whole human blood

CXCL10 -responsive gene induction was conﬁrmed using a QuantiGene 12-plex panel to test RNA isolated from whole blood of a healthy donor. Whole blood was stimulated ex vivo with C X C L10. Gene induction was shown to be dose-dependent (Figure 12) and was eliminated upon heat inactivation of the chemokine. Gene expression was normalized to the expression level of the GAPDH housekeeping gene and presented as the ratio of treated:untreated. Data courtesy of Medarex Inc., a BMS Company [5].

Beneﬁt: QuantiGene Plex assays work directly with whole blood without the need for globin depletion or RNA puriﬁcation.

Target discovery

Target identiﬁcation and

(PDX) models

Lead optimization

High-throughput screening

Secondary screening

Veriﬁcation of genes

involved with inﬂammation

Color key

−3 −2 −1 0 1 2 3 Row z-score

SCC

p63

–

p63

Histology

p63

V558

V553

V554

S100A8 KRT5 CTA-55I10 .1 TRIM29 S100A2 FAT2 CLCA2 DSG3 DSC3 TP63 CSTA DENND2C USH1C TSPAN8 KIF12 HGD AC105391.3 PROM1 DDAH1 SPINK1 EPS8L3 RGL3 IL20RB SLC44A4 TSPAN12 TFF3

V615

V524

V572

V622

V629

V562

V568

V505

V636

V548

V545

V509

V611B

FFPE analysis of genes

involved with cancer

Preclinical studies

ADME

Toxicology

Veriﬁcation of genes

in FFPE samples

CYP genes used with

human hepatocytes

Clinical studies

Biomarker veriﬁcation

Clinical trials (FFPE tissues)

Expression analysis

from whole human blood

PCA analysis of genes

involved with melanoma

0.3 µg CXCL10

1.0 µg CXCL10

1.0 µg CXCL10 Heat-inactivated

Ratio of C XC L10 -treated to untreated

CD86 IL2RACCL8IL8CXCL5 Cig5CXCL10TNFalP6IFNGIL1R 1 IFIT3

Figure 12. CX CL1 0 stimulation of whole blood for 6 hr and QuantiGene analysis of gene expression. Data source: Witte A et al.

Medarex—CXCL10 expression and biological activities in inﬂammatory bowel disease. Paper presented at: The Digestive Disease Week Meeting; 2008 May 19–21; San Diego, CA.

QuantiGene Plex assays

QuantiGene Plex assay service

Accelerate your research by outsourcing your gene expression proﬁling projects that use QuantiGene Plex assays. Let us test and analyze your samples, freeing up your valuable resources for other discovery research. Our assay service is designed to help you meet your speciﬁc project goals and timelines.

Our QuantiGene Plex assay services oer the following features and beneﬁts:

• Consistency—all assays and reagents used in the service are from Thermo Fisher Scientiﬁc

• Design support—help save time by accessing our bioinformatics team to customize your probe and panel design

• Flexibility—sample types include puriﬁed RNA and cultured cells

QuantiGene Plex data analysis software

A new cloud-based software is now available to support data analysis for QuantiGene Plex gene expression assay results. The software includes an intuitive workﬂow that guides you step by step once you have uploaded your run data from your Luminex instrument. Easily assign control and treatment groups, select QC parameters, and perform data normalization. Access the cloud-based software at apps.thermoﬁsher.com/apps/quantigene. Analyzed data can be exported to the Applied Biosystems Analysis Console (TAC) software for advanced analysis and visualization. When combined with TAC, the analysis allows for better visualization and interpretation using representations like scatter and volcano plots, hierarchical clustering, and link outs to publicly available annotations. Download a free copy of TAC software at thermoﬁsher.com/tac.

Available features:

(continued)

™

Transcripto m e

• Choice—a comprehensive list of applications

Find out more at

thermoﬁsher.com/quantigeneassayservice

Speciﬁcations

The QuantiGene Plex assay can be used for RNA proﬁling.

Feature Speciﬁcation

Limit of detection ≤1,000–2,000 transcripts/assay well Limit of quantitation ≤2,000–4,000 transcripts/assay well Linear dynamic range ≥3.0 logarithmic units Assay CV ≤15% intra-assay; ≤20% inter-assay

Compatible sample types

Assay format 96- or 384-well plate Targets/we ll 3–80

Cultured cells, bacteria, whole bood, PAXgene dried blood spots, fresh-frozen tissues (animal or plant), FFPE samples, puriﬁed RNA

• Analyze data from 96- and 384-well plates

• Rank normalization genes and calculation of P values

• Group samples and analyze fold change in groups

• Export data for advanced analysis using TAC software

during fold-change analysis

™

samples, Invitrogen™ Tempus™ samples,

O-the-shelf panels

We have also developed over 5,200 panels that cover over 22,500 genes. These available panels cover a wide range of host species that include: human, mouse, rat, canine, monkey, porcine, soy, maize, and many others. Any combination of existing genes can be combined to create

Custom panels

Custom QuantiGene Plex panels are available for any gene and any species. Custom panels ranging from 3- to 80-plex can be designed and shipped typically within 2–3 weeks. Customers simply provide us with a gene list,

RefSeq IDs, or DNA sequences. a new panel. To ﬁnd the latest panels and genes, go to thermoﬁsher.com/qgppanels or contact one of our probe designers at lsg.qgprobe@thermoﬁsher.com.

Below are some examples of the genes included in 80-plex panels

See the list of a selection of veriﬁed 80-plex panels and the rest of our 5,200 panels at thermoﬁsher.com/qgppanels.

Human Apoptosis 80-plex panel

CASP6 CARD8 BN IP2 D APK1 BAG3 H PRT1 TN FRSF10A BID TN FRSF7 LT BR TNFRSF9 BIRC8 CASP3 TP53 BIRC3 TNFR SF1A

PYCARD AK T1 BRAF TP53BP2 BAD BC L2L1 TP73 GADD45A FA DD TNF RSF21 BCL2L2 N OL3 FA SLG CASP 8 PPIB CASP4

CASP1 CASP2 B CLAF1 AB L1 TNF TNFSF10 T NF RSF 11B APAF1 TNFSF8 C ASP14 I GF1R B CL10 LTA TN FRSF10 B CASP9 BC L2 L11

TRAF4 B FAR M CL1 BIRC6 TNFSF5 BAG1 B CL2 A1 B AK1 CIDEB TRAF2 TR AF3 CFLAR C ASP10 CD40 BNIP3L RIPK2

CASP7 BAG4 BIRC1 BC L2L10 TRADD BNIP1 TNF RSF25 BA X BNIP3 BIRC2 CARD4 CARD6 CIDEA FAS BCL2 BIK

Human Oncogene 80-plex panel

NUSAP1 ER BB3 WNT2 SPARC RA B17 PRAME GJB1 SL C7A1 MCAM R NF157 ACSL3 T RPM4 C ACN A1D TOP2 A BR AF SPARCL1

MYLK BIRC5 ARPC2 C DKN1B POU2F3 C CNE1 FN1 E2F1 Z NF577 PHIP HI F1A KIF20A M AGEA1 C 10or f137 HPRT1 B UB1

CASP8 KLK3 CDKN 2B CCN D1 R B1 CDH2 YWHAZ MCM6 DSC1 MCM4 MITF VCAN SPP1 MK I67 MY RIP P CSK6

TP53 NCOA3 PCNA TFRC F10 ITG B1 CCR7 PG K1 PTEN ITGB3 MMP10 MMP9 GSTP1 MMP2 MLANA BC L2A1

RAD54B MET GCNT1 AQP3 MC M10 WNT5A D CT TBP GPC3 CDKN2A PR KCA ITGB4 CRISP3 CC NA1 GUSB P LAUR

Human Cytokine 80-plex panel

IL1F 6 IL1 F7 IFNA5 IFNA8 IL1F 8 IFN B1 IL19 CD70 I L3 I L1F10 IL17 E FAM3 B NODA L TNFSF13 GDF3 TNFSF4

BMP4 INHBA TNFSF12 BMP2 TGFB3 CS F1 BMP1 BMP6 GDF2 GDF5 GDF9 BM P7 TXLNA BMP3 TNFSF13B IL16

TNFSF14 I L1F5 G DF10 BMP8B IL24 IL9 TNF IL12 A IL22 IL13 I L17A TNFSF10 TNFSF11 TG FB1 I L7 TGFA

PDG FA IL12B TG FB2 TN FR SF11B IL1 0 BMP5 IL5 GAPDH I L18 G DF11 IFNA2 IL2 CSF2 IL20 FASLG I L1F9

IF NA1 I L4 IFNG LTA IL6 FIGF ACT B I L1B I L1A GDF8 IL15 LTB IFNK PPIB IL8 IL 21

Human Inﬂammation 80-plex panel

PYCARD MAP K3 SUGT1 MAP3K7IP1 M AP3K7 MEFV M APK12 PSTP IP1 MA PK13 TNFSF14 P EA15 RIPK2 PANX1 NLR X1 NALP1 NLRP9

TNFSF4 P2RX7 NLRP4 MAP3K7IP2 BI RC1 NLRC5 BIRC4 NLRP3 NLRP5 AIM2 BIRC2 CARD6 CASP1 CASP5 CHUK CII TA

CTSB HS P90 AA1 TR A1 IKBKB IKBKG IRF2 MAPK9 NFKB1 MAPK8 N FKBIA RAG E TNF TNFSF5 IL12 A BCL2 TNFSF11

ICEBERG CAS P4 IRF1 IL12 B MA PK1 GAPDH CXCL1 CASP8 TRAF6 CXCL2 CCL5 CCL7 REL A PTGS 2 IFNG I L6

TIRAP IRA K1 ACTB IL1B TXN IP MYD88 CAR D12 BIRC3 CCL 2 PPIB I FNB1 NOD2 IL18 B CL2 L1 HSPCB N ALP12

QuantiGene Plex assays

QuantiGene Plex panels—pathway guide

QuantiGene Plex pathway panels

Apoptosis and autophagy

Apoptosis Autophagy Unfolded protein response

Cancer signaling

Angiogenesis Angiogenic growth factors and angiogenesis inhibitors Breast cancer and estrogen receptor signaling Cancer pathway Cancer drug resistance and metabolism Cell cycle Epithelial to mesenchymal transition (EMT) Extracellular matrix and adhesion molecules Oncogenes and tumor suppressor genes Tumor metastasis

Cardiology and bone biology

Atherosclerosis Osteogenesis Skeletal muscle—myogenesis and myopathy

Epigenetics

Epigenetic chromatin modiﬁcation enzymes Epigenetic chromatin remodeling factors Polycomb group (PcG) genes and DNA methylation Transcription factors

Inﬂammation and immunology

Cell surface markers Chemokines and receptors Cytokines Cytoskeleton regulators Dendritic and antigen-presenting cells HIV infection and host response Inﬂammation Inﬂammasomes Inﬂammatory cytokines and receptors Inﬂammatory response and autoimmunity Innate and adaptive immune responses

Interferon α and β responses Interferon and receptor Interferon (IFN) and receptor T cell and B cell activation T cell activation T cell anergy and immune tolerance Th1/Th2/Th17 Th17 for autoimmunity and inﬂammation T helper cell dierentiation Toll-like receptor signaling pathway Tumor necrosis factor (TNF) ligand and receptor

Human

Mouse

• • •

• •

• • •

• •

• • •

• •

• • •

• •

• • •

• •

• • •

• •

•

• • •

• •

•

• •

• • •

• •

•

• •

• • •

• •

• • •

• •

• • •

• •

QuantiGene Plex pathway panels

Rat

Metabolism and endocrinology

Adipogenesis Fatty acid metabolism Glucose metabolism Diabetes Glycosylation Insulin signaling pathway Mitochondria Mitochondrial energy metabolism Hypertension Obesity

Neuroscience

Neurogenesis and neural stem cell Neuroscience ion channels and transporters Neurotransmitter receptors and regulators Neurotrophin and receptors

Stem cells

Embryonic stem cells Endothelial cell biology Hematopoietic stem cells and hematopoiesis Mesenchymal stem cell Stem cell Stem cell signaling Terminal dierentiation markers

Toxicity and drug metabolism

Cardiotoxicity Drug metabolism Drug metabolism phase I enzymes Drug metabolism phase II enzymes Drug transporters Hepatotoxicity Lipoprotein signaling and cholesterol metabolism Nephrotoxicity Neurotoxicity Stress and toxicity Toxicity

Housekeeping genes

Custom

Any gene for any model organism

Design a custom veriﬁed QuantiGene Plex assay at

thermoﬁsher.com/order-quantigene-plex

(continued)

Human

Mouse

• • •

• •

• • •

• •

• • •

• •

• • •

• •

• • •

• •

• • •

• •

• • •

• •

• • •

•

• • •

Rat

Analyze both protein and gene expression from a

Gro-α: Protein vs. mRNA expression

GM-CSF: Protein vs. mRNA expression

Protein mRNA expression

MCP-1: Protein vs. mRNA expression

IL-18: Protein vs. mRNA expression

SDF-1a: Protein vs. mRNA expression

Protein mRNA expression

IL-8: Protein vs. mRNA expression

IL-1b: Protein vs. mRNA expression

Protein mRNA expression

MIP-1a: Protein vs. mRNA expression

TNF-a: Protein vs. mRNA expression

IP-10: Protein vs. mRNA expression

IL-6: Protein vs. mRNA expression

Protein mRNA expression

MIP-1b: Protein vs. mRNA expression

single sample

Protein and gene expression data can be generated from the same sample, giving researchers more information about that sample. After a cell culture is centrifuged, the supernatant is analyzed for multiple cytokines using the

Centrifuged

Supernatant cell pellet

sample

ProcartaPlex assay. The cell pellet is then lysed and run using the QuantiGene Plex assay to obtain gene expression data (Figure 13).

Figure 13. Protein and gene expression data from a single sample.

Multiplex application—analysis of protein and gene expression from the same sample

500 450 400 350 300 250 200 150

Protein (pg/mL)

100

d1 unstim d1 stim d3 stim

Protein mRNA expression

4.0

3.5

3.0

2.5

2.0

1.5

1.0

0.5

0.0

mRNA (normalized MFI units)

1,400

1,200

1,000

800

600

400

Protein (pg/mL)

200

d1 unstim d1 stim d3 stim

Protein mRNA expression

14.0

12.0

10.0

8.0

6.0

4.0

2.0

0.0

mRNA (normalized MFI units)

40.0

35.0

30.0

25.0

20.0

15.0

10.0

Protein (pg/mL)

5.0

d1 unstim d1 stim d3 stim

Protein

expression

data

Gene

expression

data

Protein mRNA expression

0.006

0.005

0.004

0.003

0.002

0.001

0.000

mRNA (normalized MFI units)

1,800

1,600

1,400

1,200

1,000

800

600

Protein (pg/mL)

400

200

d1 unstim d1 stim d3 stim

Protein mRNA expression

5,000 4,500 4,000 3,500 3,000 2,500 2,000

1,500

Protein (pg/mL)

1,000

500

d1 unstim d1 stim d3 stim

350

300

250

200

150

100

Protein (pg/mL)

d1 unstim d1 stim d3 stim

120

100

Protein (pg/mL)

d1 unstim d1 stim d3 stim

Protein mRNA expression

Figure 14. Protein and gene expression analysis of several cytokines. Human PBMCs were isolated using Ficoll-Paque PLUS (GE Healthcare, Cat. No. 17-1440-02) and grown in Gibco

8.0

7.0

6.0

5.0

4.0

3.0

2.0

1.0

0.0

mRNA (normalized MFI units)

0.14

0.12

0.10

0.08

0.06

0.04

0.02

0.00

mRNA (normalized MFI units)

0.14

0.12

0.10

0.08

0.06

0.04

0.02

0.00

mRNA (normalized MFI units)

0.14

0.12

0.10

0.08

0.06

0.04

0.02

0.00

mRNA (normalized MFI units)

™

OpTimizer™ T Cell Expansion SFM (Cat. No. A102 21- 01). The cells were then stimulated with 10 µg/mL LPS for up to 72 hours. Cells were lysed using Invitrogen 72 hours (d3), and supernatants were tested for cytokine/chemokine/growth factor proteins in the Invitrogen (Cat. No. EPX650-10065-901) and in an identical biomarker conﬁguration for mRNA using the Invitrogen

1,800

1,600

1,400

1,200

1,000

800

600

Protein (pg/mL)

400

200

d1 unstim d1 stim d3 stim

Protein mRNA expression

400

350

300

250

200

150

100

Protein (pg/mL)

d1 unstim d1 stim d3 stim

250

200

150

100

Protein (pg/mL)

d1 unstim d1 stim d3 stim

Protein mRNA expression

™

QuantiGene™ Sample Processing Kit for cultured cells (Cat. No. QS0100) at 24 (d1) and

6.0

5.0

4.0

3.0

2.0

1.0

0.0

mRNA (normalized MFI units)

7.0

6.0

5.0

4.0

3.0

2.0

1.0

0.0

mRNA (normalized MFI units)

0.025

0.020

0.015

0.010

0.005

0.000

mRNA (normalized MFI units)

™

25,000

20,000

15,000

10,000

Protein (pg/mL)

5,000

d1 unstim d1 stim d3 stim

Protein mRNA expression

8,000

7,000

6,000

5,000

4,000

3,000

2,000

Protein (pg/mL)

1,000

d1 unstim d1 stim d3 stim

™

ProcartaPlex™ Human 65-plex panel

QuantiGene™ Plex Assay Kit (Cat. No. QP 1013 ).

3.5

3.0

2.5

2.0

1.5

1.0

0.5

0.0

mRNA (normalized MFI units)

1.20

1.00

0.80

0.60

0.40

0.20

0.00

mRNA (normalized MFI units)

The data show a sampling of the results for inﬂammatory chemokines (A) and Th1/ Th2 cytokines (B), and demonstrate the ease of multiplex measurement of proteins and RNA from a single sample. MFI = median ﬂuorescence intensity. Additional data can be found at thermoﬁsher.com/quantigene.

Additional gene expression solutions

Gene expression proﬁling solutions

Comprehensive solutions for gene expression proﬁling, veriﬁcation, and analysis are supported by sequencing, microarrays, and realtime PCR technologies. For discovery, in particular, de novo discovery of dierentially expressed genes (DEGs), transcriptome sequencing provides a hypothesis-neutral approach that allows interrogation of any gene in the sample, with a wide dynamic range to allow dierential gene expression analysis of low-expressing genes. For analysis deep within the known transcriptome, transcriptome-level microarrays provide fast proﬁling of coding and noncoding genes, exons, and transcript isoforms. Well-annotated gene expression analysis can be performed with gene-level microarrays. DEGs can be veriﬁed using mid-throughput realtime PCR formats such as Applied Biosystems™ TaqMan® OpenArray™ Panels and TaqMan® Array Cards, and the resulting genes of interest can be quantiﬁed more precisely by digital PCR or real-time PCR with Applied Biosystems™ TaqMan® Gene Expression Assays. Testing experiments can also be performed to further examine the behavior of these genes across a larger population of samples.

Gene expression proﬁling by sequencing

You can get the same simple workﬂow with the same ﬂexibility, now faster and more scalable than ever. The Ion Torrent™ GeneStudio™ S5 series is a line of benchtop NGS systems that enable you to eciently run small and large projects across multiple research applications, with the simplest sample-to-data benchtop NGS workﬂow and superior speed. With ﬂexibility powered by the ability to choose from ﬁve Ion Torrent™ chips, these systems oer an opportunity to conduct wide-ranging experiments on a single platform. A single instrument can scale easily to accommodate multiple RNA analysis applications, throughputs, and data outputs including:

• Whole-transcriptome analysis

• Small RNA analysis

• Targeted RNA sequencing

Find out more at thermoﬁsher.com/genestudio

Gene expression proﬁling by microarrays

Accelerate your expression biomarker discovery research with Applied Biosystems™ Clariom™ assays, the next generation of transcriptome proﬁling tools oering a fast path to results.

• Generate expression proﬁles from as little as 100 pg of total RNA—as few as 10 cells

• Analyze RNA from a wide variety of sample types, including cells, whole blood, and fresh or fresh-frozen, or FFPE tissues

• Preserve sample integrity and reduce variability with no need for globin or ribosomal RNA removal

• Transcriptome proﬁling of coding and long noncoding genes, exons, and splice variants with the Applied Biosystems™ Clariom™ D Pico Assay

• Well-annotated gene-level only expression proﬁling with the Clariom S Pico Assay

• Short noncoding RNA proﬁling with the Applied Biosystems™ GeneChip™ miRNA 4.0 Assay

Find out more at thermoﬁsher.com/transcriptomeproﬁling

Gene expression analysis by real-time PCR

Real-time PCR is the gold-standard technique for veriﬁcation of dierential gene expression proﬁles. Our comprehensive oering of TaqMan Gene Expression Assays and ﬂexible formats enable fast and cost-eective results. We are the leader in gene expression analysis, providing world-class sample preparation with Applied Biosystems™ technologies, real-time PCR using TaqMan or Applied Biosystems™ SYBR™ Green chemistry, the popular Applied Biosystems™ QuantStudio™ family of real-time PCR instruments, as well as data analysis software.

The TaqMan assay technology is the gold standard in performance, quality, and content for gene expression analysis. With predesigned TaqMan Assays, spend your time generating results, and not designing and optimizing assays.

• Detect virtually any gene product—more than 1.5 million predesigned assays, and custom designs for everything else

• Assays for nearly every human, mouse, and rat gene in the RefSeq database

• Available for 25 species, and some pathogens

• Assays for multiple locations per transcript and across nearly every exon junction in human genes

Find out more at thermoﬁsher.com/qpcr

Gene expression quantiﬁcation by digital PCR

Digital PCR enables highly precise measurements and is able to resolve changes in gene expression level of two-fold or less. Digital PCR can also be used to determine the absolute quantiﬁcation of a transcript without the need for a reference gene.

Now, you can go beyond the limits of real-time PCR with the Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System. The system is ideal for laboratories with multiple applications or end users on a limited budget. Easily interchangeable thermal cycling block formats let you select the format (96-well, 96-well Fast, and 384-well) that best suits your project without having to move attached peripherals or computers. The intuitive software, easy touchscreen setup, and eortless block changes are designed to let you get started right away.

Find out more at thermoﬁsher.com/quantstudio6ﬂex

Comprehensive immunoassay product listing

Targets across immunoassay formats

The data presented in this table are typical performance results reported on manufactured lots at the time of publication. For the most up-to-date per formance data for each individual kit, go to thermoﬁsher.com/immunoassays, and enter the

Target Species Format Volum e Sensitivity Range Cat. No.

Human Coated ELISA Serum, plasma: 50 µL; CCS: 100 µL 10 pg/mL 10–3,000 pg/mL EHTNFRSF9

4-1BB (C D137/ TNFRS F9)

4-1BB ligand (CD137L) Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 168.95–692,000 pg/mL EPX010-15901-901 6Ckine (CCL21) Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 13.27 pg/mL 13.35–54,700 pg/mL EPX0 10-1238 4-9 01

17-hydroxyprogesterone Multispecies

ACE Mouse Coated ELISA CCS, plasma, serum: 100 μL 0 .12 n g / mL 0.12 – 3 0 n g /mL EMACE Acetylcholinesterase Multispecies Activity Kit Serum, plasma, CCS: 100 μL 0.218 mU/mL 6.25–100 mU/mL EIAACHEF

Activin A

ADAM9 Human Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–12,000 pg/mL EHADAM9 ADA M11 ( M D C ) Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–400 pg/mL EHADAM11 ADA M17 ( TACE ) Human Coated ELISA CCS, plasma, serum: 100 μL 70 pg/mL 70–5,000 pg/mL EHADAM17

Adiponectin

Aggrecan (PG) Human Coated ELISA Serum, CCS, synovial ﬂuid: 50 µL 0.9 ng/mL 10– 250 ng /m L KAP 146 1 AgRP (agouti-related

protein) AHSG (fetuin A) Human Coated ELISA CCS, plasma, serum: 100 μL 0.2 ng/mL 0.2–50 ng/mL EHAHSG

AKT (total) Multispecies Coated ELISA Cell lysate: 10 µL

AKT [pS473] Multispecies Coated ELISA Cell lysate: 10 µL

AK T [pT 308 ] Multispecies Coated ELISA Cell lysate: 10 µL

AKT colorimetric in-cell Human Coated ELISA NA NA NA 6 22 15 AKT near infrared in-cell Human Coated ELISA NA NA NA 62220

AKT activation Multispecies

AKT (total/phospho) Multispecies

AK T1 (total) Human Coated ELISA Cell lysate: 10 µL 0 .1 n g / m L 0.3 –30 ng/mL KHO0531 AKT1 [pS473] Human Coated ELISA Cell lysate: 10 µL 0.1 U /mL 0.3–25 U/mL KHO0541 Albumin Human Coated ELISA CCS, plasma, serum: 100 μL 60 pg/mL 60–12,000 pg/mL EHALB ALCAM (CD166) Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–5,000 pg/mL EHALCAM

Aldosterone Multispecies

alpha-fetoprotein Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–1,800 pg/mL EHA FP alpha-synuclein Human Coated ELISA 50 μL <0.2 ng/mL 0.23–15 ng /m L KHB0061

Amphiregulin (AREG)

AMP K [pT 172] Multispecies Coated ELISA Cell lysate: 10 µL <1 U/mL 1.6 –10 0 U/mL KHO0651 Amyloid beta

(aggregated) Amyloid precursor

protein (APP)

Mouse Coated ELISA Serum, plasma: 10 µL; CCS: 100 µL 6 pg/mL 6–1,500 pg/mL EMTNFRSF9 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 7.87 pg/mL 12.21–50,000 pg/mL E PX01A-10 28 9- 901

Competitive ELISA

Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–5,000 pg/mL EHACTIVINA Rat Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–400 pg/mL ERINHBA

Mouse Coated ELISA Serum: 10 µL 50 pg/mL 0.125 – 8 n g / m L KMP0041 Rat Coated ELISA Serum, plasma: 33 µL; CCS: 100 µL 50 pg/mL 0.375–24 ng/mL KRP 00 41

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.6 pg/mL 56.15–230,000 pg/mL EPX01A-12032-901

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.3 pg/mL 82.42–337,600 pg/mL EPX01A-2603 8-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.3 pg/mL 38.4 5 –157,500 pg /mL EPX01A-42032-901

Human Coated ELISA Serum, plasma: 50 µL; CCS: 100 µL 6 pg/mL 6–1,800 pg/mL EHAGRP

Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–4,000 pg/mL EHAREG Mouse Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 8–600 pg/mL EMAREG

Human Coated ELISA

Human Coated ELISA Cell lysate, CSF: 10 µL <0.4 ng/mL 0.78–50 ng/mL KHB0051

Coated ELISA 50 µL (1:5,000 prediluted) 0.012 ng/mL 0.23–15 ng/mL BMS2032-2 ProQuantum CCS, serum, plasma: 2–5 µL <0.7 pg/mL 0.76–1,500,000 pg/mL A44838

InstantOne ELISA

Competitive ELISA

Serum, plasma, CCS: 50 μL 20.3 pg/mL 24.69–6,000 pg/mL EIA17OHP

Cell lysate: 10 µL <0.8 U/mL 1.6–10 0 U /m L 85-86049

Cell lysate: 20 µL <0.8 U/mL 1.6–10 0 U /m L 85-86046

Serum, plasma, CCS: 100 μL 4.97 pg/mL 15–4,000 pg/mL EIAALD

Supernatant, tissue homogenate: 50 µL; CSF, ventricular ﬂuid: 100 µL

Cat. No. or name of the kit. ( White rows indicate Invitrogen™ products and gray rows indicate Invitrogen correlation across available formats. Data available upon request. NA = not applicable.)

™

eBiosciences™ products. Products within each brand have biomarker

<0.1 n g / m L (1,500 cells)

<0.8 U/mL (4,000 cells)

<0.01 ng/mL 0.09 – 5.7 ng/mL KHB3491

0.3–20 ng/mL KHO0101

1.6 –10 0 U/mL KH O 0111

0–100 U/mL KHO 0201

Target Species Format Volum e Sensitivity Range Cat. No.

Amyloid beta 40

Amyloid beta 42

Amyloid beta 42 US Human

Angiogenin Human

Angiopoietin-1 Human

Angiopoietin-2 Human Coated ELISA CCS, plasma, serum: 100 µL <6 pg/mL 31.2–2,000 pg/mL KHC 1641 Angiopoietin-4 Human Coated ELISA CCS, plasma, serum: 100 μL 0.25 ng/mL 0.25–20 0 ng/mL E H A NGP T4 Angiopoietin-like 3

(ANGPTL3) Angiopoietin-like 4 Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–20,000 pg/mL EHANGPTL4

Angiostatin Human

Annexin V Human Coated ELISA CCS, serum: 50 µL 0.33 ng/mL 0.78–50 ng/mL BMS252 Anti-IFN alpha Human Coated ELISA CCS, plasma, serum: 20 µL 1.4 ng/mL 3.15–200 ng/mL B M S2 17 Apolipoprotein A1 Human Coated ELISA CCS, plasma, serum: 100 μL 0.08 ng/mL 0.08–20 ng/mL EHAPOA1 Apolipoprotein C1 Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3– 50,000 pg/mL EHAPOC1 Apolipoprotein C2 Human Coated ELISA CCS, plasma, serum: 100 μL 1. 5 ng /m L 1.5 –100 ng/mL EH APOC2 Apolipoprotein C3 Human Coated ELISA CCS, plasma, serum: 100 μL 2.5 pg/mL 2.5–10,000 pg/mL EHAPOC3 Apolipoprotein E (AD2) Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 ng /mL 1.5 – 4 00 n g/m L EHAPOE Apolipoprotein E3 Human Coated ELISA CCS, plasma, serum: 100 μL 0.4 ng/mL 0.4–100 ng/mL EHAPOE3

Apolipoprotein E4 Human

APRIL Human

Arginase-1 Human

Atrial natriuretic peptide (ANP)

AXL

B7- H 6 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 51.186 pg/mL 118,51–485,400 pg/mL EPX010-1232 8-9 01 BACE1

(Beta-secretase 1)

BAFF

BAFF R (TNFRSF13C) Mouse Coated ELISA CCS, plasma, serum: 100 μL 28 pg/mL 28–7,000 pg/mL EMTNFRSF13C B2M (beta-2-

microglobulin) BCAM (basal cell

adhesion molecule; CD239)

Bcl-2 Human Coated ELISA Cell lysate, plasma, serum: 20 µL 0.5 ng/mL 0.50–32 ng/mL BMS244-3 BCL2L2 (Bcl-2-like

protein 2; BCL-W) BCL2L2 (Bcl-2-like

protein 2; BCL-W)

BDNF

beta-catenin (total) Human Coated ELISA Cell lysate: 10 µL <0.30 ng/mL 0.78–50 ng/mL K HO12 11 Betacellulin (BTC) Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.4 pg/mL 30–122,900 pg/mL EPX01A-26042-901 beta-secretase 1 Human Coated ELISA CCS, plasma, serum: 100 μL 1.0 ng /mL 1.0 –250 ng/mL EHBACE1

Human

Mouse Coated ELISA CCS, CSF, tissue homogenate: 10 µL <5 pg/mL 7.81–500 pg/mL KMB3481

Human

Mouse Coated ELISA CCS, CSF, tissue homogenate: 10 µL <3 pg/mL 3.12–200 pg/mL KMB3 441

Mouse Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40 –10,000 pg/mL EMANGPTL3

Multispecies

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–250 pg/mL EMAXL

Human ProcartaPlex Serum, plasma, CSF: 25 μL 410.098 pg/mL 200–817,400 pg/mL EPX010 -123 58 -901

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 2.88–11,800 pg/mL EPX01A-26096-901

Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–1,000 pg/mL EHB2 M

Human Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40–10,000 pg/mL EHBCAM

Human Coated ELISA CCS, plasma, serum: 100 μL 60 pg/mL 60–16,000 pg/mL EHBCL2L2

Rat Coated ELISA CCS, plasma, serum: 100 μL 0.4 ng/mL 0.4–100 ng/mL ERBCL2L2

Rat Coated ELISA CCS, plasma, serum: 100 μL 12 pg/mL 12–3,000 pg/mL ERBDNF Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.02 pg/mL 2.44–10,000 pg/mL EPX 01A-12116- 90 1 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.02 pg/mL 2.44–10,000 pg/mL EPX01A-42116-901

Coated ELISA CCS, CSF, tissue homogenate: 50 µL <6 pg/mL 9.8–500 pg/mL KHB3481 ProcartaPlex Serum, plasma, CSF: 25 μL 242.9 pg/mL 451–1,847,000 pg/mL E PX0 10-1235 0-901

Coated ELISA CCS, CSF, tissue homogenate: 50 µL <10 pg/mL 15.6 –1,0 00 pg/mL KHB3441 ProcartaPlex Serum, plasma, CSF: 25 μL 0.3 pg/mL 0.4–1,700 pg/mL EPX010 -12351-901

Ultrasensitive Coated ELISA

Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–400 pg/mL EHANG ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.07 pg/mL 0.1–600 pg/mL EPX010 -12234 -9 01 Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–18,000 pg/mL EHANGPT1 ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 22.99 pg/mL 32.4 0 –132,700 pg/mL EPX010-12235- 90 1

Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–2,000 ng/mL EHANGIOSTATIN ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 2.86 pg/mL 1.3–5,150 pg/mL EPX010 -12237-9 01

Coated ELISA CCS, plasma, serum: 100 μL 0.4 ng/mL 0.4–100 ng/mL EH APOE4 ProcartaPlex Serum, plasma, CSF: 25 μL 14.747 pg/mL 195–800,000 pg/mL EPX010 -123 44-901 ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.78 pg/mL 90.70–371,500 pg/mL EPX01A-12008-901 Coated ELISA Serum, plasma: 50 µL 0.4 ng/mL 0.78–50 ng/mL BMS2008 ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 9.87 pg/mL 12.21–50,000 pg/mL EPX010-12216 -901 Coated ELISA Serum, plasma, CCS: 50 μL 20.81 pg/mL 78.1–5,000 pg/mL BMS2216 Competitive

ELISA Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–2,000 pg/mL EHAXL Coated ELISA CCS, plasma, serum: 50 μL 7.46 pg/mL 31.25–2,000 pg/mL BMS2286 ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 241.8 pg/mL 244–1,000,000 pg/mL EPX010 -122 86 -901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 2.9–11,800 pg/mL EPX01A-12007-901 Instant ELISA CCS, plasma, serum: 50 µL 0 .13 n g / m L 0.31–20 ng/mL BMS2007INST

CCS, CSF, tissue homogenate: 25 µL <1 pg/mL 1.56–100 pg/mL KHB3544

Serum, plasma, CCS: 50 μL 0.25 ng/mL 0.7–180 ng/mL EIAANP

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

Big E T-1 Human

Blood urea nitrogen (BUN)

BMP-2 (bone morphogenetic protein 2)

BMP-4 (for lysates) (bone morphogenetic protein 4)

BMP-4 (bone morphogenetic protein 4)

BMP-5 (bone morphogenetic protein 5)

BMP-6 (bone morphogenetic protein 6)

BMP-7 (bone morphogenetic protein 7)

BMP-9 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 8.22 pg/mL 6.47–25,600 pg/mL EPX 010 -12246-9 01 BTC (betacillin) Human Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–18,000 pg/mL EHBTC

BTL A (CD272)

Butyrylcholinesterase Multispecies Activity Kit Serum, plasma, CCS: 100 µL 0.018 m U/mL 0.3–20 mU/mL EIABCHEF

C1q Human Coated ELISA

C3a Human

C5a

CA19-9 (carbohydrate antige n 19 -9)

CA9 (carbonic anhydrase IX)

Calcitonin Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.74 pg/mL 2.23–9,150 pg/mL EPX01A-12067-901 Cardiotrophin 1 Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–12,000 pg/mL EHCTF1 Cardiotrophin 1 (CT-1) Mouse Coated ELISA CCS, plasma, serum: 100 μL 200 pg/mL 0.2–60 ng/mL EMCTF1

Caspase-3 Human

Caspase 3 (cleaved) colorimetric in-cell

Caspase 3 (cleaved) near infrared in-cell

Caspase-3 (active) Human Coated ELISA Cell lysate: 10 µL 0.033 ng/mL 0.039–2.5 ng/mL KHO109 1 Caspase-8 Human Coated ELISA CCS, plasma, serum: 50 µL 0.1 n g / m L 0.16 –1 0 ng/mL BMS2024 Caspase-9 Human Coated ELISA CCS, plasma, serum: 50 µL 0.4 ng/mL 1.6–100 n g/mL BMS2025 Catalase Multispecies Activity Kit Serum, plasma, CCS: 25 μL 0.052 U/mL 0 .15 –5 . 0 U/m L E IACATC Cathepsin L Human Coated ELISA CCS, serum: 50 µL 1.7 n g/mL 3.1– 50 ng/mL BMS257 Cathepsin S (CTSS) Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–1,000 pg/mL EHCTSS CCL1 (I-309) Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–3,000 pg/mL EHCC L1 CCL1 (TCA-3) Mouse Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–8,000 pg/mL EMCCL1

CCL3 (MIP-1 alpha)

CCL4 (MIP-1 beta)

CCL6 (C10) Mouse Coated ELISA CCS, plasma, serum: 100 μL 18 pg/mL 18–1,000 pg/mL EMCCL6

Multispecies Detection Kit Serum, plasma, CCS: 50 μL 0.030 mg/dL 0.156–10 mg/dL EIABUN

Human

Human Coated ELISA Tissue lysates, cell lysates: 100 μL 15 pg/mL 15–6,000 pg/mL EHBMP4CL

Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–6,000 pg/mL EHBMP4

Human Coated ELISA CCS, plasma, serum: 100 μL 250 pg/mL 0.25 –60 ng/mL EHBMP5

Human Coated ELISA CCS, plasma, serum: 100 μL 150 pg/mL 150–50,000 pg/mL EHBMP6

Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–6,000 pg/mL EHBMP7

Mouse Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–1,000 pg/mL EMBMP7

Human

Mouse ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 2.4 pg/mL 13–53,600 pg/mL EPX010-26052-901

Mouse Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–400 pg/mL EMHC

Human Coated ELISA

Human Coated ELISA CCS, plasma, serum: 100 μL 0.3 U/mL 0.3–200 U/mL E HC A199

Human Coated ELISA CCS, plasma, serum: 100 μL 0.2 pg/mL 0.2–50 pg/mL EHCA9

Multispecies Coated ELISA NA NA NA 62218

Multispecies Coated ELISA NA NA NA 62223

Human Instant ELISA CCS, plasma, serum: 50 µL <6 pg/mL 15.6 –1,000 pg/mL BMS2029INST Mouse Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–1,000 pg/mL EMCCL3

Human

Human ProQuantum CCS, serum, plasma: 2–5 µL <0.6 pg/mL 0.64-10,000 pg/mL A35616 Human Instant ELISA CCS, plasma, serum: 50 µL 3.0 pg/mL 7.8–500 pg/mL BMS2030INST

Human

Human ProQuantum CCS, serum, plasma: 2–5 µL <0.05 pg/mL 0.064– 5,000 pg/mL A35597

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.47 pg/mL 0.50–2,050 pg/mL EPX010 -122 66 -901 Coated ELISA Serum, plasma, CCS: 50 μL 0.185 pg/mL 0.4–25 pg/mL BMS2266

Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–3,000 pg/mL EHB MP2 ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 5.966 pg/mL 52 – 211,100 pg /mL E PX0 10-12319 -9 01

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 38.21 pg/mL 122.7–500,000 pg/mL EPX01A-12217-901 Coated ELISA CCS, plasma, serum: 25 µL 16.7 pg/mL 78.13–5,000 pg/mL BMS 2217

Serum, plasma: 10 µL (1:100 prediluted) CCS: 50 µL

Coated ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.6 pg/mL 12.2–5,000 pg/mL E PX010-12 282-9 01

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.59 pg/mL 4.88–20,000 pg/mL EPX01A-12012-901 Instant ELISA CCS, plasma, serum: 10 µL 0 .12 n g / m L 0 .16 –10 n g /mL BMS2012INST

Uncoated ELISA

Plasma, serum: 10 µL (prediluted 1:500 for plasma, 1:30 for serum ) CCS: 50 µL

Serum: 5 µL; plasma: 10 µL; CCS: 50 µL

CCS, plasma, serum: 100 µL 16 pg/mL 16–2,000 pg/mL 88-7035 -22

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-70 34 -22

0.08 ng/mL 0.9–60 ng/mL BMS2099

0.07 ng/mL 0.3–20 ng/mL BMS2 089

0.005 ng/mL 0.078–5.0 ng/mL BMS20 88

Target Species Format Volum e Sensitivity Range Cat. No.

Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–200 pg/mL EHCCL8

CCL8 (MCP-2)

CCL9 (MIP-1 gamma) Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–250 pg/mL EMCCL9 CCL12 (MCP-5) Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.5 pg/mL 0.5–100 pg/mL EMCCL12 CCL13 (MCP-4) Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–200 pg/mL EH CCL13 CCL14 (HCC-1) Human Coated ELISA CCS, plasma, serum: 100 μL 7 pg/mL 7–1,500 pg/mL EHC C L14 CCL15 (MIP-1 delta) Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–1,000 pg/mL EHC CL15 CCL16 (HCC-4) Human Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–6,000 pg/mL EH CCL16 CCL17 (TARC) Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–2,500 pg/mL EHC C L17 CCL17 (TARC) Mouse Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–3,000 pg/mL EMCCL17 CCL18 (PARC) Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–2,000 pg/mL EHC CL18

CCL19 (MIP-3 beta)

CCL20 (MIP-3 alpha) Human Coated ELISA Tissue lysates, cell lysates: 100 μL 1.5 pg/mL 1.5–600 pg/mL EHCCL20CL CCL20 (MIP-3 alpha) Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–600 pg/mL EHCCL20 CCL20 (MIP-3 alpha) Mouse Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–1,500 pg/mL EMCCL20

CCL21 (SLC) Human

CCL21A (6Ckine; SLC) Mouse Coated ELISA Serum, plasma: 50 µL; CCS: 100 µL 20 pg/mL 20–1,000 pg/mL EMCCL21A CCL23 (MPIF-1) Human Coated ELISA CCS, plasma, serum: 100 μL 7 pg/mL 7–6,000 pg/mL EHCCL23 CCL25 (TECK) Human Coated ELISA CCS, plasma, serum: 100 μL 200 pg/mL 0.2–150 ng /mL EHCCL 25 CCL25 (TECK) Mouse Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–2,000 pg/mL EMCCL25

CD14 Human

CD23 (sCD23/FCER2) Human Coated ELISA CCS, plasma, serum: 100 µL 6.8 U/mL 12.5 – 400 U/mL BMS 22 7/2

CD26 (DPP4)

CD27

CD28 Human

CD30 Human

CD30L (TNFSF8)

CD31 Human Instant ELISA CCS, plasma, serum: 10 uL 0.06 ng/mL 0.47–30 ng/mL BMS229INST

CD36

CD40

CD40L

CD44 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 85.63 pg/mL 49.90–204,400 pg/mL EPX010 -102 09 -901

CD44std Human

CD44var (v5) Human

CD44var (v6) Human

Human

Human ProQuantum CCS, serum, plasma: 2–5 µL <0.01 pg/mL 0.0128–5,000 pg/mL A35599

Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–500 pg/mL EH CCL19 Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–1,000 pg/mL EMCCL19

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.45 ng/mL 0.45–100 ng/mL EMDPP4 Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–3,000 pg/mL BMS235

Human

Mouse ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1.7 pg/mL 5–21,900 pg/mL EPX010-26 053-901

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 185.13–758,300 pg/mL EPX010 -102 98 -9 01 Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.5 pg/mL 0.5–100 pg/mL EMTNFSF8

Mouse Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–20,000 pg/mL EMCD36 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 136.7 pg/mL 436–1,786,000 pg/mL E PX010-12 28 9- 901 Mouse Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–1,500 pg/mL EMCD40

Human

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 5.7–23,400 pg/mL EPX 01A-10239-9 01 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6 pg/mL 5.3–21,800 pg/mL EPX01A-40239-901

Human

Mouse Coated ELISA CCS, serum: 50 µL 0.14 n g / m L 0.31–20 ng/mL BMS 6010

Uncoated ELISA

Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6 –6,000 pg/mL EH CD14 ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 10.28 pg/mL 34.40–140,900 pg/mL EPX010-12283- 90 1

Instant ELISA CCS, plasma, serum: 100 μL 0.5 pg/mL 0.5–100 pg/mL BMS286INST ProcartaPlex CCS, plasma, serum: 100 μL 30 pg/mL 30–20,000 pg/mL EPX01A-102 86- 901

Coated ELISA CCS, serum: 50 μL 0.2 ng/mL 0.78–50 ng/mL BMS290 ProcartaPlex CCS: 50 μL, plasma, serum: 25 μL 0.44 ng/mL 0.5–2,000 ng/mL E PX0 1A-10 29 0-901 Coated ELISA CCS, plasma, serum: 25 μL 0.3 ng/mL 1.6 –100 ng/ mL BMS240 ProcartaPlex CCS, plasma, serum: 100 μL 20 pg/mL 20 –15,000 pg /m L EPX 01A-1024 0-901 Instant ELISA CCS, plasma, serum, urine: 25 µL 0.3 ng/mL 1.6 –10 0 ng /mL BMS240INST

Coated ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.98 –12,200 pg/mL EPX01A-1026 5-9 01

Coated ELISA CCS, plasma, serum: 15 µL 0.01 ng/mL 0.8–5 ng/mL BMS293 Instant ELISA CCS, plasma, serum: 20 µL 0.062 ng/mL 0.16 –1 0 ng /mL BMS239INST

Coated ELISA 20 µL (1:60 prediluted) 0.02 ng/mL 0.13 – 4 ng /mL BMS209 -2 Instant ELISA 20 µL (1:60 prediluted) 0.016 n g/mL 62.5–4,000 pg/mL BMS209INST Coated ELISA 20 µL (1:6 prediluted) 0 .14 n g /mL 0.31–10 ng /m L BMS220 Instant ELISA CCS, plasma, serum: 10 µL 0.01 ng/mL 0 .16 –10 n g /mL BMS220INST ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.13 pg/mL 24.4–100,000 pg/mL E PX0 1A-10210 -9 01

Coated ELISA

Instant ELISA CCS, plasma, serum, urine: 10 µL 0.06 ng/mL 0.16 –10 ng/m L BMS210INST

CCS, plasma, serum: 10 µL 0.16 pg/mL 0.16–10 pg/mL 8 8-52169 -2 2

CCS, plasma, serum: 100 µL 63 pg/mL 63–4,000 pg/mL 88- 52 107

CCS, plasma, serum: 50 µL 40.6 pg/mL 40.6–2,600 pg/mL 88 -58 214

Amniotic ﬂuid, CCS, plasma, serum: 50 µL

Plasma, serum, urine: 10 µL; CCS: 20 µL

1.3 pg/mL 7.8–500 pg/mL BMS265

0.05 ng/mL 0.32–10 ng/mL B MS210

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

CD47 (IAP) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.84 pg/mL 6.13–25,100 pg/mL EPX010 -12329 -901 CD48 (BLAST-1) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 18.7 pg/mL 24.42–116,400 pg/mL E PX0 10-1233 0-9 01

CD80

CD80 (B7-1) Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 14.33 pg/mL 30.52–125,000 pg/mL EPX01A -102 91- 901

CD93 Human

CD134 (OX40)

CD137 (s4-1BB) Human Coated ELISA CCS, serum: 50 µL 0.2 ng/mL 0.78–50 ng/mL BMS289 CD137L (4-1BBL) Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 7.7 pg/mL 20–81,300 pg/mL EPX010-26058-901

CD152 (CTLA-4) Human

CD163 (M13 0) Human Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–8,000 pg/mL EHCD163 CD200 Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–6,000 pg/mL EHCD200 CD226 (DNAM-1) Human Coated ELISA CCS, plasma, serum: 100 μL 5 ng/mL 5 –600 ng/mL EHCD226 CD252 (OX40 L/TNFSF4) Mouse Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–8,000 pg/mL EMTNFSF4

CD276 Human

CD2 76 ( B7-H 3 )

CD278 (ICOS) Human Coated ELISA CCS, plasma, serum: 100 μL 1.2 ng/mL 1. 2–3 0 0 n g /m L EHICOS CEACAM1 (BGP) Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20 –15,000 pg /m L EHCEACAM1

CEACAM5 (CEA) Human

Ceruloplasmin Multispecies Activity Kit Serum, plasma, CCS: 100 μL 3.26 mU/mL 31.2–1,000 mU/mL EIACPLC CFD (Adipsin) Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–6,000 pg/mL EHCFD CHI3L1 (chitinase 3-like

1; Y KL- 40 )

CKMB (CKM/CKB) Human

Clusterin Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–20,000 pg/mL EHCLU Clusterin (Apo-J) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 276.003 pg/mL 220–900,000 pg/mL EPX010-1234 5-9 01 Clusterin (CLU) Rat Coated ELISA CCS, plasma, serum: 100 μL 1.3 ng/ mL 1.3–320 n g/m L ERCLU

c-Met Human

c-Myc Human Coated ELISA Cell lysate: 5 µL <5 pg/mL 15.6 –1,0 00 pg/mL KHO2 041

CNTF (ciliary neurotrophic factor)

COMP Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 725.9 pg/mL 3 57–1,461,80 0 pg /mL EPX010-12317-9 01

Corticosterone Multispecies

Cortisol Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL

Cortisol Multispecies

Cortisone Multispecies

C-peptide Human

Creatinine urinary Multispecies Detection Kit Serum, plasma, CCS: 50 μL 0.019 m g/dL 0.3–20 mg/dL EIACUN

CREB (total) Multispecies Coated ELISA Cell lysate: 10 µL

CREB [pS133] Multispecies Coated ELISA Cell lysate: 10 µL <0.5 U/mL 0.8–50 U/mL K HO0241

Human Instant ELISA CCS, plasma, serum: 50 µL 0 .1 n g /mL 0.31–20 n g/m L BMS291INST Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 7.8 pg/mL 72–296,200 pg/mL EPX010-26056-901

Uncoated

ELISA Human Coated ELISA CCS, serum: 25 µL 1.8 pg/mL 78–5,000 pg/mL BMS29 6 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.984 pg/mL 7,52–30,800 pg/mL EPX010-12333-901

Coated ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 4.72 pg/mL 12.21–50,000 pg/mL EPX01A-10 276 -901

Uncoated

ELISA Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 164.1 pg/mL 388–1,588,800 pg/mL EPX010-26059-901 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 255.73 pg/mL 142.75 –584,700 pg /mL EPX 010 -12326 -9 01

Coated ELISA CCS, plasma, serum: 100 µL 200 pg/mL 0.2–250 ng/mL EHCEA

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–25,000 pg/mL A4 63 41

Human

Rat Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–2,000 pg/mL ERCNTF

Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–1,500 pg/mL EHCHI3L1

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 13.743 pg/mL 36–148,700 pg/mL EPX010-12322-9 01

Coated ELISA CCS, plasma, serum: 100 μL 0.3 ng/mL 0.3–80 ng/mL EHCKMB

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.7 pg/mL 3–11,300 pg/mL EPX010 -122 64 -9 01

Coated ELISA Cell lysate: 10 µL 0.4 ng/mL 0–50 pg/mL KHO0251

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–25,000 pg/mL A46338

Coated ELISA CCS, plasma, serum: 100 μL 200 pg/mL 0.2–200 ng/mL EHCNTF

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.479 pg/mL 2–7,150 pg/mL EPX010 -123 41-901

Competitive

ELISA

Competitive

ELISA

Competitive

ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 48.83–200,000 pg/mL EPX 010 -12191-901

Coated ELISA CCS, plasma, serum: 50 μL 4.12 pg/mL 8.6–550 pg/mL B MS 2191

CCS, plasma, serum: 10 µL 3.3 ng/mL 3.3–400 ng/mL 8 8-50340-22

Amniotic ﬂuid, CCS, plasma, serum: 10 µL

CCS, plasma, serum: 10 µL 0.16 ng /mL 0.1 6 –10 n g /mL 88-50370

TCS, serum, plasma, urine: 50 μL (1:10 0 )

Serum, plasma, CCS: 50 μL 17.3 pg/mL 100 –3,200 pg/mL EIAHCOR

Serum, plasma, CCS: 50 μL 29.0 pg/mL 100–100,000 pg/mL EIACOR

0.1 3 ng/mL 0.16 –10 ng /mL BMS276

18.6 pg/mL 78.1–10,000 pg/mL EIACORT

<0.025 ng/mL

(10

cells)

4,882.8– 20,000,000 pg/mL

0.1 6 –10 n g /mL KHO0231

EPX010 -12190- 901

Target Species Format Volum e Sensitivity Range Cat. No.

Human Coated ELISA Plasma, serum: 5 µL <10 pg/mL 18.75–1,200 pg/mL KHA00 31 Rat Coated ELISA CCS, plasma, serum: 100 μL 200 pg/mL 0.2–60 ng/mL ERCRP

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.29 pg/mL 4–18,000 pg/mL EPX01A-10 28 8-901

CRP (C-reactive protein)

CRT (CALR) Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 309.8 pg/mL 764–3,130,900 pg/mL E PX0 10-12 29 5-901

CTACK (CCL27)

CT F-1 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 8.86 pg/mL 11.35–46,500 pg/mL EPX0 10-12 25 4- 901 CTLA-4 (CD152) Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.1 pg/mL 9–38,500 pg/mL EPX010-26060 -901 CTLA-4 (cytotoxic

T-lymphocyte protein 4)

Cu/ ZnSOD1 Human Coated ELISA

CX CL13 (BL C)

CX CL14 (B R A K ) Human Coated ELISA CCS, plasma, serum: 100 μL 700 pg/mL 700–21,000 pg/mL EHCXCL14 CX CL15 Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.8 ng/mL 0.8–200 ng/mL EMCXCL15 CX CL16 Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–2,000 pg/mL EHCXCL16 CX CL16 Mouse Coated ELISA CCS, plasma, serum: 100 μL 1.3 pg/mL 1.3–80 pg/mL EMCXCL16 CXCL2 (CINC-3) Rat Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–1,800 pg/mL ERCXCL2 CXCL6 (GCP-2) Human Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–2,500 pg/mL EH CXCL6

Cyclic AMP Multispecies

Cyclic GMP Multispecies

Cystatin C Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 25.25 pg/mL 27.98–114,600 pg/mL EPX010-12279-901 Cystatin (CST3) Human Coated ELISA CCS, plasma, serum: 100 μL 300 pg/mL 0.3 –20 ng/mL EHCST3 Cystatin (CST3) Mouse Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–3,200 pg/mL EMCST3 Cystatin (CST3) Rat Coated ELISA CCS, plasma, serum: 100 μL 1.2 ng/ mL 1.2–750 n g/mL ERCST3 Cytochrome c Human Coated ELISA Cell lysate: 10 µL < 0 .15 6 n g / m L 0.078–5 ng/mL K HO 1051 DCN (decorin) Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–700 pg/mL EHDCN DCN (decorin) Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–1,000 pg/mL EMDCN DcR3 (TNFRSF6B) Human Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 0.3 ng/mL 0.3–80 ng/mL EHTNFRSF6B

D-dimer

Dehydroepiandrosterone sulfate (DHEA-S)

DIABLO (SMAC) Human Coated ELISA CCS, plasma, serum: 100 μL 100 pg/mL 0.1– 30 ng/mL EHDIABLO DKK-1 Human Coated ELISA CCS, plasma, serum: 100 μL 100 pg/mL 100–30,000 pg/mL EHD KK1 DKK-1 Mouse Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25–6,000 pg/mL E MDKK1 DKK-3 Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–2,000 pg/mL EHDKK3 DKK-4 Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10 –10,0 00 pg/m L EHDKK4 DLL1 Human Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–12,000 pg/mL E HD LL1 DMP1 Human Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 80 –40,000 pg/mL EHD MP1

DNA damage Multispecies

DR5 (TNFRSF10B) Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–600 pg/mL EHTNFRSF10B DR6 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 33.19 pg/mL 17.41–71,300 pg/mL EPX01A-15904-901

E-cadherin Human

E-cadherin (CDH1) Mouse Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–3,000 pg/mL EMCDH1

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 40.62–166,400 pg/mL EPX01A-26 045-901

Rat

Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–10,000 pg/mL EHCCL27 Mouse Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 15 pg/mL 15 –18,00 0 pg /mL EMCCL27A Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.3 pg/mL 7.23–29,600 pg/mL EPX01A-12188- 901

Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–1,200 pg/mL EMCTLA4

Mouse Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–2,000 pg/mL EMCXCL13 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5 pg/mL 6.3–25,800 pg/mL EPX01A-12147-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 60 pg/mL 37.89–155, 20 0 pg/mL EPX01A-42147-901

Human Coated ELISA CCS, plasma, serum: 100 μL 0.08 pg/mL 0.08–60 pg/mL EHDDIMER

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 132 pg/mL

Multispecies

Coated ELISA Serum, plasma: 100 µL 150 pg/mL 0.15 –10 n g/mL 88-75 02-28

Instant ELISA 100 µL (1:500 prediluted) 3 pg/mL 78–5,000 pg/mL BMS288INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 2.44–10,000 pg/mL EPX01A-36047-901

Coated ELISA

Competitive

ELISA

Competitive

ELISA

Competitive

ELISA

Competitive

ELISA

Coated ELISA 100 μL 100 ng/mL 100–2,700 ng/mL 991700

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 25.07 pg/mL 36.28–148,600 pg/mL EPX010-12315 -901

Serum, plasma: 1 µL; urine, CCS: 100 µL

Amniotic ﬂuid, fetal umbilical vein blood, CCS, Plasma, serum, urine: 10 µL

CCS, CL, TH: 100 µL

Serum, plasma, CCS: 50 μL 90.9 pg/mL 96–60,000 pg/mL EIADHEA

Serum, plasma, CCS: 50 μL 50.9 pg/mL 62.5–8,000 pg/mL EIADNAD

2 ng/mL 2–133 n g/mL 8 8-750 1-28

0.04 ng/mL 0.8–5 ng/mL BMS222

0.39 pmol/mL (nonacetylated);

0.04 pmol/mL (acetylated)

0.6 pmol/mL (nonacetylated);

0.06 pmol/mL (acetylated)

0.78–200 pmol/mL (nonacetylated);

0.08–20 pmol/mL (acetylated)

0.8–500 pmol/mL (nonacetylated);

0.08–50 pmol/mL (acetylated)

317.9 9 –1, 3 0 2 , 5 0 0 p g / mL

EMSCAMPL

EMSCGMPL

EPX01A-12149-901

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

ED A-1 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.35 pg/mL 12.55–51,400 pg/mL EPX01A-15903-901

NHP Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–2,000 pg/mL EPEGF Mouse Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–800 pg/mL EMEGF Rat Coated ELISA CCS, plasma, serum: 100 μL 0.8 pg/mL 0.8–200 pg/mL EREGF

EGF

Human

EGFR (full-length) Human Coated ELISA Cell lysate: 10 µL

EGFR [pY1173] Human Coated ELISA Cell lysate: 10 µL <0.3 U/mL 1.67–100 U/mL KHR9071 EGFR [pY1068] Human Coated ELISA Cell lysate: 10 µL 1. 6 U /m L 1.67–100 U/mL KHR9081 EGFR colorimetric in-cell Multispecies Coated ELISA NA NA NA 62205 EGFR near infrared

in-cell EG -VEG F (PROK1) Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10 –10,00 0 pg/mL EHPROK1 Elaﬁn Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 5.24 pg/mL 1.83–7,500 pg/mL E PX0 10-12 248- 90 1 EMMPRIN Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 4.59 pg/mL 4.74–19,400 pg/mL E PX010-12242-9 01

ENA-78 (CXCL5/LIX)

Endocan (ESM1) Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.1 n g / m L 0 .1– 3 0 ng /mL EMESM1

Endoglin (CD105)

Endostatin Human

Endothelin-1 (ET-1) Human Coated ELISA Serum, plasma, CCS: 50 μL 0.579 pg/mL 0.78–100 pg/mL EIAET1 ENC1 (CCL28) Human Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25–20,000 pg/mL EHENC1

Eotaxin (CCL11)

Eotaxin-2 (CCL24)

Eotaxin-3 (CCL26) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.01 pg/mL 0.3–1,400 pg/mL EPX01A-12140-901 EPCAM (TROP1) Human Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–12,000 pg/mL EHEPCAM Epiregulin (EREG) Mouse Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–6,000 pg/mL EMEREG EPO receptor Human Coated ELISA CCS, plasma, serum: 100 μL 180 pg/mL 187.5–12,000 pg/mL EHEPOR

ERK 1/2 (total/phospho) Multispecies

ERK 1/2 (total) Multispecies

ERK1/2 colorimetric in-cell

ERK1/2 near infrared in-cell

Erythropoietin (EPO) Human

Multispecies Coated ELISA NA NA NA 62210

Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–18,000 pg/mL EHCXCL5 Mouse Coated ELISA CCS, plasma, serum: 100 μL 35 pg/mL 35–2,000 pg/mL EMCXCL5

Rat

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.67 pg/mL 11.1–45,400 pg/mL EPX01A-26043-901 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 8.06–33,000 pg/mL EPX01A-12119 -901

Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–3,000 pg/mL EHENG

Human

Human Coated ELISA CCS, plasma, serum: 50 µL <2.2 pg/mL 7.8–500 pg/mL KAC2231

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–100 pg/mL EMCCL11

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 1.9–7,700 pg/mL EPX01A-36008-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.4 pg/mL 7.56–31,000 pg/mL EPX01B-42120-901 Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–1,000 pg/mL EHCCL24 Mouse Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–800 pg/mL EMCCL24 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.9 pg/mL 3.42–14,000 pg/mL EPX01A-12150-901

Multispecies Coated ELISA NA NA NA 6220 6

Multispecies Coated ELISA NA NA NA 62 2 11

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.85 pg/mL 2.93–12,000 pg/mL EPX01A-12070-901

Instant ELISA CCS, plasma, serum: 50 µL 0.26 pg/mL 3.91–250 pg/mL BMS2070INST

Coated ELISA

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35579

Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20– 6,000 pg/mL ERCXCL5

Coated ELISA Tissue lysates, cell lysates: 100 μL 15 pg/mL 15–6,000 pg/mL ERCXCL5CL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.09 pg/mL 0.61–2,500 pg/mL EPX0 1A-12105 -9 01

Instant ELISA CCS, plasma, serum: 10 µL 2.6 pg/mL 15.6–1,000 pg /mL BMS2105INST

Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–10,000 pg/mL EHCOL18A1

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 4.19 pg/mL 5–194,000 pg/mL EPX010 -12238 -901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.4 pg/mL 0.61–2,500 pg/mL E PX 01B -12120 -90 1

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.64–5,000 pg/mL A35580

Coated ELISA CCS, serum: 50 µL 3.6 pg/mL 31.3–2,000 pg/mL BMS6008

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.01 pg/mL 0.5–2,000 pg/mL EPX01A-26008-901

InstantOne

ELISA

InstantOne

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 0.17 mIU/mL 1.6–100 mIU/mL BMS2035

Coated ELISA CCS, serum, plasma: 50 µL 0.14 mIU/mL 1.6–100 mIU/mL BMS2035-2

ProQuantum CCS, plasma, serum: 2–5 μL <1 mIU/mL 1.28–20,000 mIU/mL A 40419

CCS, plasma, serum: 100 µL; urine: 10 µL

Cell lysate: 20 µL 85-86013

Cell lysate: 20 µL 85-86011

<1 pg/mL 3.9–250 pg/mL KHG0062

<0.08 ng/mL

(10

cells)

0.1 6 –10 n g /mL KHR9061

Target Species Format Volum e Sensitivity Range Cat. No.

Mouse Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–3,000 pg/mL EMSELE

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 555.3 pg/mL 0.2 9 –1,20 0 ng /mL EPX01A -102 05 -901

E-Selectin (CD62E)

Estradiol (17-betaestradiol)

Estrone Multispecies

Estrone-3-glucuronide (E1G)

Estrone-3-sulfate (E1S) Multispecies

FABP-2 (intestinal) Human Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 0. 025–100 ng /mL EHFABP2 FABP-3 (cardiac) Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 ng/mL 1. 5–2 00 n g/m L EHFABP3

FABP-3 Human

Factor X Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL EPX01A-12152-901 FAK (total) Multispecies Coated ELISA Cell lysate: 10 µL <1.6 ng /mL 1.6–10 0 n g /m L KHO0431 FAK [ pY397] Multispecies Coated ELISA Cell lysate: 10 µL <1.0 U/mL 1.6–100 n g/mL KHO0441 FAP Human Coated ELISA CCS, plasma, serum: 100 μL 12 pg/mL 12–4,000 pg/mL EH FAP

Fas (APO/CD95) Human

Fa s -L ( CD178)

Fc epsilon RI Human Coated ELISA Serum, plasma, CCS: 20 µL 0 .14 n g /mL 0.078–5.00 ng/mL BMS 2101 Fc gamma R2B

(FCGR2B) Fc gamma R2BC

(FcGR2BC; CD32 B/C) Ferric antioxidant status

(FRAP)

Ferritin Human

Fetuin-A Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 57.7 pg/mL 98–400,800 pg/mL EPX010-12347- 90 1 FETUB (fetuin B) Human Coated ELISA CCS, plasma, serum: 100 μL 4 ng/mL 4–5 ng/mL EHFETUB FGF-1 (FGF-alpha;

ﬁbroblast growth factor 1 (acidic))

FGF-2

FGF-4 (for lysates) Human Coated ELISA Cell, tissue lysates: 100 μL 50 pg/mL 50–18,000 pg/mL EHFGF4CL FGF-4 Human Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 50 pg/mL 50–18,000 pg/mL EHFGF4 FGF-6 Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6 –6,000 pg/mL EHFGF6 FGF-7 (KGF; keratinocyte

growth factor) FGF-9 Human Coated ELISA CCS, plasma, serum: 100 μL 200 pg/mL 200–35,000 pg/mL EHFGF9 FG F -19 Human Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–8,000 pg/mL EHFGF19 FGF-21 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.4 pg/mL 9–35,400 pg/mL E PX010-1235 7-9 01 FGF-23 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 48.83–200,000 pg/mL E PX010-1220 4- 901 FGF-Basic (bFGF; FGF-2) Human Coated ELISA CCS, plasma, serum: 50 µL 7 pg/mL 15.6 –1,000 pg/mL KHG0021 Fibrinogen Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 350 pg/mL 1.22–5,000 ng/mL EPX01A-12153-901

Fibronectin Human Coated ELISA

FIGF (VEGF-D; vascular endothelial growth factor D)

FLT1 (VEGF R1; VEGF receptor 1)

Human

Monkey

Human Coated ELISA Plasma, serum: 50 µL 5 pg/mL 10–1,000 pg/mL K AQ0 621

Multispecies

Rat Coated ELISA CCS, plasma, serum: 100 μL 90 pg/mL 90–60,000 pg/mL ER FASLG

Human

Mouse Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 80 pg/mL 0.08–70 ng/mL EMFCGR2B

Human Coated ELISA CCS, plasma, serum: 100 μL 85 pg/mL 85–5,000 pg/mL EHFCGR2B

Multispecies Detection Kit Serum, plasma, CCS: 20 μL 8.06 µM 31.2–1,000 µM EIAFECL2

Human Coated ELISA CCS, plasma, serum: 100 μL 12 pg/mL 12–4,000 pg/mL EHFGF1

Mouse Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–600 pg/mL EMFGF2 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.47 pg/mL 4.9–20,000 pg/mL EPX01A-42074-901 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.47 pg/mL 4.9–20,000 pg/mL EPX01A-12074-901

Human Coated ELISA CCS, plasma, serum: 100 μL 0.5 pg/mL 0.5–400 pg/mL EHFGF7

Human Coated ELISA CCS, plasma, serum: 100 μL 0.2 ng/mL 0.2–20 ng/mL EHFIGF

Mouse Coated ELISA

Coated ELISA CCS, plasma, serum: 20 µL 0.3 ng/mL 0.8–50 ng/mL BMS205

Instant ELISA CCS, plasma, serum: 20 µL 0.33 ng/mL 1.56–50 ng /mL BMS205INST

Coated ELISA CCS, serum: 20 µL 0.4 U/mL 1.9–60 U/mL BMS6 49

Instant ELISA CCS, plasma, serum: 20 µL 0.6 U/mL 1.88–60 U/mL BMS649INST

Competitive

ELISA

Competitive

ELISA

Competitive

ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 551.38 pg/mL 146.48–600,000 pg/mL EPX010-12263 -9 01

Coated ELISA CCS, plasma, serum: 50 μL 26.88 pg/mL 156.3–5,000 pg/mL BMS2263

Coated ELISA CCS, Serum: 10 μL 13.2 pg/mL 15.6 –1,0 00 pg/mL BMS245TEN

ProcartaPlex

Coated ELISA

ProcartaPlex

Coated ELISA CCS, plasma, serum: 100 μL 600 pg/mL 0.6–200 ng/mL EHF TL

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 132.377 pg/mL 242–991,100 pg/mL EPX010 -1218 4-901

Serum, plasma, CCS: 50 μL 22.4 pg/mL 31.2–2,000 pg/mL EI A17E1

Serum, plasma, CCS: 50 μL 7.38 pg/mL 15.6 –1,0 00 pg/mL E IA17E 3

Serum, plasma, CCS: 50 μL 26.4 pg/mL 41–4,000 pg/mL EIA17 E3S

Serum, plasma: 25 µL; CCS: 50 µL

Amniotic ﬂuid, CCS, plasma, serum: 50 µL

Serum, plasma: 25 µL; CCS: 50 µL

Plasma, serum: 10 µL; CCS, urine: 50 µL

Serum, plasma: 50 µL; CCS: 100 µL

26.61 pg/mL 61.035–250,000 pg/mL EPX01A-102 45 -901

0.07 ng/mL 0.1 6 –10 n g /mL BMS260-2

0.7 pg/mL 2.2–9,000 pg/mL E PX01A-10260- 90 1

0.1 n g / m L 0.31–20 ng/mL BMS2028

8 pg/mL 4.14–3,000 pg/mL EMF LT1

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–400 pg/mL EH FLT3 LG

FLT- 3L ( fms -re lat ed tyrosine kinase 3 ligand)

Follistatin Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 19.13 pg/mL 19.85–81,300 pg/mL EPX010 -12244 -9 01 Formaldehyde Multispecies Detection Kit Serum, plasma, CCS: 50 μL 0.715 µM 3.1–200 µM EIACH2O

Fractalkine (CX3CL1)

FST (follistatin) Human Coated ELISA CCS, plasma, serum: 100 μL 500 pg/mL 0.5 –125 n g/mL EHFST FSTL3 (FLRG) Human Coated ELISA CCS, plasma, serum: 100 μL 250 pg/mL 250 –50,000 pg/mL EHFSTL3 Furin Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–10,0 00 pg/mL EHFURIN Galactose Multispecies Detection Kit Serum, plasma, CCS: 20 μL 0.493 mg/dL 0.78–25 m g/dL EIAGALC Galectin-1 (LGALS1) Mouse Coated ELISA CCS, plasma, serum: 100 μL 12 pg/mL 2–10 f old EMLGALS1

Galectin-3 (LGALS3)

Ga le ctin-7 Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–10,000 pg/mL EHLGALS7 Galectin-7 (LGAL27) Mouse Coated ELISA CCS, plasma, serum: 100 μL 65 pg/mL 65–16,000 pg/mL EMLGALS7 Galectin-9 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.298 pg/mL 2,22–9,100 pg/mL EPX010 -123 31-901

GAPDH Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 120 pg/mL

GAS6

GCP-2 (CXCL6) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.96 pg/mL 1.06–4,350 pg/mL EPX010 -123 86 -901

G-CSF (CSF-3)

GDF -15 (gr o w th dierentiation factor 15)

GDNF Human

GFAP Human ProcartaPlex Serum, plasma, CSF: 25 μL 11.528 pg/mL 8–32,900 pg/mL EPX 010 -12336 -901

GITR (CD357)

GITRL

Glucose Multispecies Detection Kit Serum, plasma, CCS: 20 μL 0.413 mg /d L 0.5–32 mg/dL EIAGLUC

Glutathione Multispecies Detection Kit Serum, plasma, CCS: 50 μL

Glutathione reductase Human Activity Kit Serum, plasma, CCS: 25 μL 0.009 mU/mL 0 .15 –5 m U /mL EIAGRF Glutathione S-transferase Multispecies Activity Kit Serum, plasma, CCS: 50 μL 2.70 mU/mL 7.8–500 mU/mL EIAGSTF

Human

Monkey Coated ELISA CCS, plasma, serum: 100 μL 12 pg/mL 12–400 pg/mL EPFLT3 LG Mouse Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–5,000 pg/mL E M FLT 3 L

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 300 pg/mL 0.3–200 ng/mL EMCX3CL1

Rat

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.5 pg/mL 2.08–8,500 pg/mL E PX 01A -12121- 901

Mouse Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–2,000 pg/mL EMLGALS3 Rat Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–800 pg/mL ERLGALS3

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–5,000 pg/mL EMGAS6

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.5 pg/mL 0.5 –150 pg/mL EMCSF3

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.19 pg/mL 1.4–5,850 pg/mL EPX01A-26034-901 Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 3.7–15,000 pg /m L EPX01A-36034-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.8 pg/mL 4.44–18,200 pg/mL EPX01A-42001-901

Human

Human Coated ELISA Tissue lysates, cell lysates: 100 μL 15 pg/mL 15 –10,000 pg /m L EHTNFRSF18CL

Human

Human Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25–10,000 pg/mL EHTNFSF 18 Mouse Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–1,000 pg/mL EM TN FS F18 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 12.41 pg/mL 54.74–224,200 pg/mL EPX01A-15902-901

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064–25,000 pg/mL A35581

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 2.11–8,650 pg/mL EPX010-12387-9 01

Coated ELISA CCS, plasma, serum: 100 μL 300 pg/mL 0.3–60 ng/mL EHCX3CL1

ProQuantum CCS, plasma, serum: 2–5 μL <0.005 pg/mL 0.0064–500 pg/mL A35582

Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–2,000 pg/mL ERCX3CL1

Coated ELISA Tissue lysates, cell lysates: 100 μL 5 pg/mL 5–2,000 pg/mL ERCXCL1CL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 213.9 pg/mL

Coated ELISA CCS, plasma, serum: 50 µL 0.29 ng/mL 0.47–30.0 ng/mL BMS279-4

Coated ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 45.4 pg/mL 83–338,500 pg/mL EPX0 10-12 291-9 01

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.4 pg/mL 16.46–67,400 pg/mL EPX01A-12001-901

Instant ELISA CCS, plasma, serum: 50 µL 11 pg/mL 39.1–2,500 pg/mL BMS2001INST

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 9.65 pg/mL 12.21–50,000 pg/mL EPX010-1225 8-901

Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–800 pg/mL EHGDF15

Coated ELISA

Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–2,000 pg/mL EHGDNF

ProcartaPlex Serum, plasma, CSF: 25 μL 47.254 pg/mL 58–238,400 pg/mL E PX0 10-12 34 0-901

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 13.36 pg/mL 24.41–100,000 pg/mL E PX0 1A-12210 -9 01

Coated ELISA CCS, plasma, serum: 25 µL 9.7 pg/mL 62.3–4,000 pg/mL BM S2 210

CCS, plasma, serum: 50 μL (1:200 prediluted)

CCS, plasma, serum: 50 μL (1:20 prediluted)

0.65 pg/mL 11.7–750 pg/mL BMS2291

0.59 pg/mL 2–125 pg/mL BMS2258

0.634 µM 0.78–25 µM EIAGSHC 45 nM 0.2–25 µM EIAGSHF

439.45–1,800,000 pg/mL

244.14–1,000,000 pg/mL

EPX01A-10279-901

EPX01A-12154-901

Target Species Format Volum e Sensitivity Range Cat. No.

Porcine Coated ELISA CCS, plasma, serum: 100 μL 72 pg/mL 72–18,000 pg/mL ESCS F2

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.2 pg/mL 17.09–70,000 pg/mL EPX01A-102 83 -901

ProcartaPlex

Uncoated Human

GM-CSF (CSF-2)

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.3 pg/mL 9.8–40,000 pg/mL EPX01A-30612-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.2 pg/mL 6.37–26,100 pg/mL EPX01A-40283-901

g p13 0 ( I L-6 RB ) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.96 pg/mL 14.43–59,100 pg/mL E PX010-12 376 -901

Granzyme A Human

Human

Granzyme B

Ghrelin Human

GLP -1 Human

GLP 7- 3 6a Human Coated ELISA CCS, plasma, serum: 100 μL 150 pg/mL 150–20,000 pg/mL EHGLP Glucagon (GCG) Human Coated ELISA CCS, plasma, serum: 100 μL 2.5 pg/mL 2.5 –130 pg/mL EHGCG GREM2 (PRDC) Mouse Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–800 pg/mL EMGREM2 GRN (progranulin) Mouse Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–15,0 00 pg/mL EMGRN

GRO alpha (KC/CINC1/ CX CL1)

Growth stimulationexpressed protein ST2

HAVCR1 (TI M -1) Human Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 2 pg/mL 2–3,000 pg/mL EHHAVCR1 HAVCR1 (TI M -1) Mouse Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 30 pg/mL 30–8,000 pg/mL EMHAVCR1 HAVCR1 (TI M -1) Rat Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 10 pg/mL 10–3,000 pg/mL ERHAVCR1

HB-EGF Human

hCG (chorionic gonadotropin)

HER-2 Human

HER3 (ERBB3) Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–2,500 pg/mL EHERBB3 Hemoglobin Multispecies Detection Kit Serum, plasma, CCS: 10 μL 0.021 g/dL 0.25–16 g /dL EIAHGBC

HGF

Mouse

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 117.4–120,200 pg/mL EPX01A-42027-901

Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–150 pg /m L EMCXCL1

Rat Coated ELISA CCS, plasma, serum: 100 μL

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.05 pg/mL 1.7–7,000 pg/mL EPX01A-26031-901 Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.4 pg/mL 11.4–46,500 pg/mL EPX01A-36031-901

Human Coated ELISA Serum, plasma, CCS: 50 µL 30.5 pg/mL 31.25–2,000 pg/mL EHIAST2

Human Coated ELISA CCS, plasma, serum: 100 μL 50 pg/mL 50–40,000 pg/mL EHCG

Human Coated ELISA CCS, plasma, serum: 50 µL <20 pg/mL 125–8,000 pg/mL KAC2211

Mouse

Human

ELISA

Coated ELISA

Instant ELISA CCS, plasma, serum: 50 µL 0.6 pg/mL 7.8–500 pg/mL BMS283INST

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A42891

Coated ELISA CCS, serum: 50 µL 2.0 pg/mL 3.9–250 pg/mL BMS 612

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.19 pg/mL 2.4–10,000 pg/mL EPX01A-20612-901

ProQuantum CCS, serum, plasma: 2–5 μL <0.3 pg/mL 0.32–25,000 pg/mL A46735

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 23.3 pg/mL 8.54–35,000 pg/mL E PX0 10-1223 2-901

Coated ELISA CCS, plasma, serum: 50 μL 0.73 pg/mL 7.8–500 pg/mL BMS2232

Coated ELISA CCS, serum: 50 µL 0.2 pg/mL 0.66–480 pg/mL BMS2027

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 117.4–120,200 pg/mL EPX01A-12027-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 1.6 pg/mL 7.8–500 pg/mL BMS 6029

ProQuantum CCS, serum, plasma: 2–5µL <0.3 pg/mL 0.32–25,000 pg/mL A44238

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 195.31–800,000 pg/mL EPX010-12192- 901

Coated ELISA CCS, plasma, serum: 25 µL 11.8 pg/mL 15.6 –1,00 0 p g/mL B MS219 2

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 9.76–40,000 pg/mL EPX010 -12194- 901

Coated ELISA CCS, plasma, serum: 50 µL 0.32 pg/mL 0.8– 50.0 pg/mL BM S2194

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.8 pg/mL 2.44–10,000 pg/mL E PX 01A -12122-9 01

Coated ELISA CCS, plasma, serum: 50 µL 7.6 pg/mL 16–1,000 pg/mL BMS2122

ProQuantum CCS, serum, plasma: 2–5 μL <0.01 pg/mL 0.0128–1,000 pg/mL A42896

Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–4,000 pg/mL EHHBEGF

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1.16 pg/mL 1.28–5,250 pg/mL E PX0 10-1224 5- 901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.8 pg/mL 10.81–44,300 pg/mL EPX01A -102 07-9 01

Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 0.03 ng/mL 0.04–2.50 ng/mL BMS20 7-2

Coated ELISA CCS, plasma, serum: 100 μL 400 pg/mL 0.4–60 ng/mL EMHGF

Coated ELISA Tissue lysates, cell lysates: 100 μL 400 pg/mL 0.4– 60 ng/mL EMHGFCL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.1 pg/mL 8.79–36,000 pg/mL EPX01A-12069-901

Instant ELISA CCS, plasma, serum: 50 µL 5.5 pg/mL 63–4,000 pg/mL BMS2069INST

Serum, plasma: 25 µL; CCS: 50 µL 1.3 pg/mL 1.79–7,330 pg/mL

CCS, plasma, serum: 100 µL 6 pg/mL 6–750 pg/mL 88-8337

Amniotic ﬂuid, CCS, plasma, serum: 50 µL

CCS, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-7334

CCS, plasma, serum: 100 µL 40 pg/mL 40–5,000 pg/mL 88-8022

2.9 pg/mL 7.8–500 pg/mL BMS283

300 pg/mL in assay diluent D and 50 pg/mL in assay diluent B

300–15,000 pg/mL ERCXCL1

EPXS010-10 28 3901

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

hGH (human growth hormone)

HIF-1 alpha Human Coated ELISA CCS, plasma, serum: 50 µL <30 pg/mL 81.92–20,000 pg/mL EHIF1A Histone demethylase Human Activity Kit Serum, plasma, CCS: 100 µL 0.128 μ M 0.128–0.64 μM EIAHDMF

HSP27 (HSPB1) Human Coated ELISA

HSP60 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 107.049 pg/mL 120–491,000 pg/mL EPX010-12 323- 90 1

HSP70 (HSPA4) Human Coated ELISA

HSP90 alpha Human Coated ELISA 100 µL (1:25 prediluted) 0.03 ng/mL 0.156–10 ng/mL BMS2090 HTRA2 (OMI) Human Coated ELISA CCS, plasma, serum: 100 μL 200 pg/mL 0.2–50 ng/mL EHHTRA2 HVEM (CD270;

TN FRS F14) I-309 (CCL1) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.167 pg/mL 0.73–3,000 pg/mL EPX010 -1237 7-9 01

IC A M -1

ICAM-2 Human Coated ELISA

ICAM-3 Human Coated ELISA

ICOS ligand (B7-H2) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.48 pg/mL 9.03–37,000 pg/mL EP X010 -123 25 -901 IDO Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 4.2 pg/mL 4.88–20,000 pg/mL EPX01A-12213-901

IFN–alpha

IFN–beta

Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–600 pg/mL EHGH1

Human Coated ELISA Plasma, serum: 50 µL 0.17 µ I U /mL 0.45–98 µIU/mL KAQ108 1

CCS, cell lysate, plasma, serum: 10 µL

CCS, Cell lysate, plasma, serum: 50 µL

Human

Rat Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–7,000 pg/mL ERICAM1

Human

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 3.98–16,300 pg/mL EPX01A-36048-901

NHP

Human

Mouse

Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.008 pg/mL 0.8–3,300 pg/mL EPX01A-66046-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 1.41–5,800 pg/mL EPX01A-40216-901 Human Coated ELISA CCS: 50 µL 50 pg/mL 50–4,000 pg/mL 414101

Mouse

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.68 pg/mL 8.54–35,000 pg/mL EPX01A-12088-901

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 3.49 pg/mL 24.41–100,000 pg/mL E PX01A-12 218 -901

Coated ELISA CCS, plasma, serum: 50 µL 7.89 pg/mL 31.25–2,000 pg/mL BM S2 218

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 76.3 pg/mL 151.85–622,000 pg/mL EPX01A-10201-901

Coated ELISA CCS, plasma, serum: 10 µL 2.2 ng/mL 6.25 –100 ng/mL BMS201

Instant ELISA

Coated ELISA CCS, serum: 10 µL 1.1 U/mL 6. 3–100 U/mL BMS64 8

Instant ELISA CCS, plasma, serum: 25 µL 1.05 U/ mL 1. 56–100 U/mL BMS648INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.4 pg/mL 83.23–340,900 pg/mL EPX01A-40201-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.61–2,500 pg/mL EPX0 1A-10216 -9 01

Coated ELISA CCS, plasma, serum: 20 µL 3.2 pg/mL 7.8–500 pg/mL BMS2 16

Instant ELISA CCS, plasma, serum: 20 µL 3.3 pg/mL 39–2,500 pg/mL BMS216INST

ProQuantum CCS, serum, plasma: 2–5 µL <0.05 pg/mL 0.064– 5,000 pg/mL A42897

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.03 pg/mL 5.6–22,800 pg/mL EPX01A-26027-901

Coated ELISA CCS, plasma, serum: 50 µL 7.48 pg/mL 31.3–2,000 pg/mL BMS6027

ProQuantum CCS, serum, plasma: 2–5 µL <1 pg/mL 1.6–25,000 pg/mL A46736

Coated ELISA CCS: 100 µL <15.6 pg/mL 15.6 –1,0 00 pg/mL 424001

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.10–25,000 pg/mL EPX01B-26044-901

Amniotic ﬂuid, bile, CCS, plasma, serum, urine: 10 µL

Amniotic ﬂuid, CCS, urine, whole blood: 100 µL; Plasma, serum: 15 µL

Amniotic ﬂuid, CCS, plasma, serum, urine: 20 µL

10.9 pg/mL 15 .6 –1,0 00 pg/mL BMS2086

0.052 ng/mL 0.156–10 ng/mL BMS2087

2.17 n g / m L 1.56–10 0 n g/m L BMS201INST

0.3 U/mL 0.75–24 U/mL BMS236

0.38 ng/mL 0.78–50 ng/mL BMS2 18

Target Species Format Volum e Sensitivity Range Cat. No.

Semi-

quantitative Bovine

Canine Coated ELISA CCS, plasma, serum: 100 μL 0.1 n g /mL 0.1– 2 5 n g /mL ECIFNG

Chicken

Porcine

Human

IFN–gamma

Mouse

Rat

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.6 pg/mL 1.02–4,200 pg/mL EPX01A-40228-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.1 pg/mL 6.37–26,100 pg/mL EPX01A-50501-901 Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.245 pg/mL 4.98–20,400 pg/mL EPX01A-66047-901

IFN–omega Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 1.5 ng/mL 1. 5 – 50 0 ng/mL EMIGA

Human

IgA

Mouse

Rat

Human

IgE

Mouse

IGF-1 (insulin-like growth factor 1)

IG F -1 Rat Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–7,500 pg/mL ERIGF1 IG F -1R Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–6,000 pg/mL EHIG F1R IGF-2 Mouse Coated ELISA CCS: 100 μL 1,500 pg/mL 1.5 –150 ng/mL EM IGF2 IGFBP-1 (insulin-like

growth factor binding pr otei n 1)

IGFBP-2

IGFBP-3

IGFBP-4 Human Coated ELISA CCS, plasma, serum: 100 μL 250 pg/mL 0.25–100 ng/mL EHIGFBP4

Mouse Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–2,000 pg/mL EMIGF1

Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–2,000 pg/mL EHIGFBP1

Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20– 6,000 pg/mL EHIGFBP2 Mouse Coated ELISA CCS, plasma, serum: 100 μL 600 pg/mL 0.6–600 ng/mL EMIGFBP2 Human Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 80–18,000 pg/mL EHIGFBP3 Mouse Coated ELISA CCS, plasma, serum: 100 μL 70 pg/mL 70–40,000 pg/mL EMIGFBP3

coated plate

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

ProcartaPlex

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 12.21–50,000 pg/mL EPX01A-10 22 8-901

Coated ELISA CCS, plasma, serum: 50 µL 0.99 pg/mL 1.6–100 pg/mL BMS228

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 µL <0.01 pg/mL 0.0128–5,000 pg/mL A35576

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.09 pg/mL 1.2–5,000 pg/mL EPX01A-20606-901

ProcartaPlex

Coated ELISA CCS, serum: 50 µL 5.3 pg/mL 15.6 –1,0 0 0 p g/mL BMS60 6

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064–10,000 pg/mL A4 115 0

Uncoated

ELISA

Instant ELISA CCS, plasma, serum: 50 µL 4 pg/mL 15.6–1,00 0 pg /mL BMS606INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 9.8–40,000 pg/mL EPX01A-30621-901

Coated ELISA CCS, serum: 50 µL 9.9 pg/mL 31.3–2,000 pg/mL BMS621

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 5.49 –22,500 pg/mL EPX0 1A-10 23 3- 901

Coated ELISA CCS, plasma, serum: 25 µL 1.5 pg/mL 4.7–300 pg/mL BMS233

Uncoated

ELISA

Coated ELISA Plasma, serum: 20 µL 0.03 ng/mL 1.6 –10 0 ng /mL BMS209 6

ProQuantum CCS, serum, plasma: 2–5 µL <0.1 n g / m L 0.128–2,000 ng/mL A45729

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

Coated ELISA Plasma, serum: 10 µL 0.5 ng/mL 7.8–500 ng/mL BMS2097

ProQuantum CCS, serum, plasma: 2–5 µL <10 pg/mL 192–3,000,000 pg/mL A46690

Uncoated

ELISA

Coated ELISA Plasma, serum: 2 µL; CCS: 100 µL 0.14 ng/m L 0.14–100 ng/m L EMIGHE

CCS, plasma: 100 µL

CCS, plasma, serum: 100 µL <31.3 pg/mL 31.2–2,000 pg/mL ESS0026B

CCS, plasma: 100 µL <15.6 pg/mL

CCS, plasma: 100 µL <15.6 pg/mL 15.6–1,00 0 pg/mL CSC4033

Serum, plasma: 25 µL; CCS: 50 µL 0.075 pg/mL 0.31–1,250 pg/mL

CCS, plasma, serum: 50 µL 0.06 pg/mL 0.16–10 pg/mL BMS228HS

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-7316

Serum, plasma: 25 µL; CCS: 50 µL 0.024 pg/mL 0.06–260 pg/mL

CCS, plasma, serum: 20 µL 0.09 pg/mL 0.63–40 pg/mL BMS609

CCS, plasma, serum: 100 µL 0.7 pg/mL 0.7–100 pg/mL 88-8314

Plasma, serum: 10 µL 1.6 n g/mL 1.6 –10 0 ng /mL 88-50600

CCS, plasma, serum: 10 µL 0.39 ng/mL 0.39 –25 ng/mL 88-50450

Plasma, serum: 10 µL; CCS: 50 µL 16 ng /mL 16–1,000 ng/mL 8 8-5048 0

CCS, plasma, serum: 10 µL 7.8 n g / m L 7.8–500 ng/mL 88- 50 610

CCS: 400 µL 4 ng/mL 4–250 ng/mL 88-50640-22

Semiquantitative

Semiquantitative K BC1231

15.6 –1,000 pg/mL

CAC1233

EPXS010-10 228901

EPXS010-20606901

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

Human Coated ELISA CCS, plasma, serum: 100 μL 2 ng/mL 2–800 ng/mL EHIGFBP5

IGFBP-5

IGFBP-6

IgG subclass Human

IgG total

IgG1

IgG2 Human

IgG2a

IgG2b

IgG2c Mouse

IgG3

IgG4 Human

IgM

IR (total) Human Coated ELISA Cell lysate: 10 µL 0.5 ng/mL 0.94–60 ng/mL KH R 9111 IR [ pY115 8 ] Multispecies Coated ELISA Cell lysate: 10 µL <0.8 U/mL 0 –10 0 U/mL KHR9121 IR [ pYpY11 62/11 6 3] Multispecies Coated ELISA Cell lysate: 10 µL <0.8 U/mL 0–100 U/m L KHR9131 IR [pY13 34] Multispecies Coated ELISA Cell lysate: 10 μL <1 U/mL 0 –10 0 U/mL KHR9161 IRS1 [pS312] Multispecies Coated ELISA Cell lysate: 10 μL <1 U/mL 0 –10 0 U/mL KHO0521

Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–3,000 pg/mL EMIGFBP5 Rat Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25–7,000 pg/mL ERIGFBP5 Human Coated ELISA CCS, plasma, serum: 100 μL 150 pg/mL 150–60,000 pg/mL EHIGFBP6 Mouse Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–6,000 pg/mL EMIGFBP6

Uncoated

ELISA

Uncoated Human

Mouse

Rat

Human

Mouse

Rat

Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.6 ng/mL 0.6–150 ng/mL EMIGG2A

Mouse

Rat

Mouse

Rat

Human

Mouse

Human

Mouse

Rat

ELISA

Coated ELISA Plasma, serum: 10 µL 0.24 ng/mL 1.6 –100 ng/mL BMS20 91

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

Coated ELISA Plasma, serum: 10 µL 0.32 ng/mL 15.6 –1,00 0 n g/mL BMS2 092

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

Coated ELISA Plasma, serum: 20 µL 0.25 ng/mL 1.2–75 n g/m L BMS2093

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

Uncoated

ELISA

Coated ELISA Plasma, serum: 10 µL 0.21 ng/mL 4.7– 300 ng/mL BMS2094

Uncoated

ELISA

Uncoated

ELISA

Coated ELISA Plasma, serum: 20 µL 0 .1 n g / m L 9.4–600 ng/mL BMS2095

ProQuantum CCS, serum, plasma: 2–5 µL <50 pg/mL 0. 08–1,250 ng/mL A45247

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 10 µL 1 ng/mL 15.6 –1,0 00 ng/mL BMS2098

ProQuantum CCS, serum, plasma: 2–5 µL <10 pg/mL 16–250,000 pg/mL A45249

Uncoated

ELISA

Uncoated

ELISA

Serum: 10 µL 991000

Plasma, serum: 10 µL 1.6 n g/mL 1.6 –10 0 ng /mL 88-50550

CCS: 50 µL; plasma, serum: 10 µL 1.56 ng/mL 1.56 –10 0 n g /m L 88-50400

CCS, plasma, serum: 10 µL 1.6 ng /mL 1.6–100 n g/mL 88-50490

Plasma, serum: 10 µL 0 .16 n g / m L 0 .16 –10 ng/mL 88-50 560

CCS: 50 µL; plasma, serum: 10 µL 3.13 ng /mL 3.13–200 ng/mL 88- 50 410

CCS: 50 µL; plasma, serum: 10 µL 6.25 ng/mL 6.25–400 ng/mL 88-50500

Plasma, serum: 10 µL 0.63 ng/mL 0.63–40 ng/mL 88-50570

CCS: 100 µL; plasma, serum: 10 µL 3.9 ng/mL 3.90–250 ng/mL 88-50420

CCS, plasma, serum: 10 µL 0.39 ng/mL 0.39 –25 ng/mL 88- 50 510

CCS: 50 µL; plasma, serum: 10 µL 3.9 ng/mL 3.90–250 ng/mL 88-50430

CCS, plasma, serum: 10 µL 2 ng/mL 2–125 ng /m L 88-50520

CCS: 50 µL; plasma, serum: 10 µL 0.39 ng/mL 0.39–25 ng/mL 88- 50670

Plasma, serum: 10 µL 4.7 ng/mL 4.7– 3 00 ng/ mL 88- 505 80

50 µL (1:10,000 prediluted) 1.875 ng /m L 1.875–125 ng /mL 88 -50440

CCS, plasma, serum: 10 µL 46.9 ng/mL 46.9–3,000 ng/mL 88-50590

CCS, plasma, serum: 10 µL 15.6 ng /m L 15.6–1,000 ng /m L 88 -50620

CCS: 50 µL; plasma, serum: 10 µL 0.39 ng/mL 0.39–25 ng/mL 88-50 470

CCS, plasma, serum: 10 µL 0.3125 ng /mL 0.31–20 ng/mL 88-50540

Target Species Format Volum e Sensitivity Range Cat. No.

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 0.61–2,500 pg/mL E PX0 1A-10243-901

IL-1 alpha

I L-1 be ta

I L-1 R 1 ( I L-1R A; IL-1 receptor type 1)

I L-1 R 2 (I L- RB ; I L-1 receptor type 2)

IL-1 R4 (IL1RL1; IL-1 receptor-like 1)

IL-1RA ( IL1RN)

Human

Mouse

Rat

Bovine

Canine Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 10 pg/mL 10–8,000 pg/mL EC IL1B Porcine Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–6,000 pg/mL ES IL1B

Human

Mouse

Rat

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.95–3,900 pg/mL EPX01A-40224-901 Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.092 pg/mL 3.73–15,3 00 pg/mL EPX01A-66048-901 Monkey Coated ELISA CCS, plasma, serum: 50 μL 0.3 pg/mL 3.9–250 pg/mL BMS645-2

Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–2,000 pg/mL E HIL1R 1

Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–3,000 pg/mL EH IL1R2

Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–1,200 pg/mL EH IL1RL1

Equine Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–3,000 pg/mL E EIL1R N

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 17.8 pg/mL 114.8–470,200 pg/mL EPX01A-42080-901

Coated ELISA CCS, plasma, serum, urine: 50 µL 1.1 pg/mL 1.6–100 pg/mL BMS243-2

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A35592

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.32 pg/mL 3.5–14,300 pg/mL EPX01A-20611-901

Uncoated

ELISA

Coated ELISA Supernatant, serum: 50 µL 4 pg/mL 7.8–500 pg/mL BMS 6 11

ProQuantum CCS, plasma, serum: 2–5 μL <0.3 pg/mL 0.32–5,000 pg/mL A42894

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.5 pg/mL 12.2–50,000 pg/mL EPX01A-30611-901

Coated ELISA CCS, serum: 50 μL 4 pg/mL 15.6 –1,0 00 pg/mL BMS627

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 2.44–10,000 pg/mL EPX01A-10224-901

ProcartaPlex

Coated ELISA CCS, plasma, serum, urine: 50 µL 0.3 pg/mL 3.9–250 pg/mL BMS224-2

Instant ELISA CCS, plasma, serum: 50 µL 0.7 pg/mL 7.8–500 pg/mL BMS224INST

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 µL <0.05 pg/mL 0.064–10,000 pg/mL A3 5 574

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.14 pg/mL 1.2–5,000 pg/mL EPX01A-26002-901

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 1.2 pg/mL 7.8–500 pg/mL BMS6002

Instant ELISA CCS, plasma, serum: 100 µL 3 pg/mL 3.9–250 pg/mL BMS6002INST

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 4 pg/mL 31.3–2,000 pg/mL BMS630

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 15.9–65,000 pg/mL EPX01A-36002-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 17.8 pg/mL 34.18–140,000 pg/mL EPX01A-12080-901

Coated ELISA CCS, plasma, serum: 50 µL 30 pg/mL 109–7,000 pg/mL BMS2080

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 88- 50 19

CCS, serum: 100 µL Undetermined 31–2,000 pg/mL ESS0027

Serum, plasma: 25 µL; CCS: 50 µL 0.145 pg/mL 0.32–1,330 pg/mL

CCS, plasma, serum, urine: 50 µL 0.05 pg/mL 0.16–10 pg/mL BMS224HS

CCS, cell lysate, plasma, serum: 100 µL

CCS: 100 µL 39 pg/mL 39–5,000 pg/mL 88-6010-22

25 pg/mL 25–3,000 pg/mL 88-8014-22

EPXS010-10 224901

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

Bovine Coated ELISA CCS, plasma, serum: 100 μL 0.2 ng/mL 0.2–200 ng/mL EBIL2 Equine Coated ELISA CCS, plasma, serum: 100 μL 0.2 ng/mL 0.2–160 pg/mL EEIL2 Human Coated ELISA CCS, plasma, serum, urine: 50 µL <6 pg/mL 38.4–1,500 pg/mL EH2IL2

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.8 pg/mL 4.88–20,000 pg/mL EPX01A -10221-901

I L- 2

I L- 2 R ( C D2 5 )

I L- 2 R A ( I L-2 r e ce pt o r subunit alpha)

I L- 2 R B ( IL- 2 r ec epto r subunit beta)

IL-3

ProcartaPlex

Uncoated

ELISA

Human

Mouse

Rat

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.05 pg/mL 0.46–1,900 pg/mL EPX01A-40221-901

Monkey Coated ELISA

Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 7.32–30,000 pg/mL EPX01A-50504-901 Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–4,000 pg/mL EHIL2RA

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 5.2–21,300 pg/mL EPX01A-20212-901

Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–4,000 pg/mL EHIL2RA

Human Coated ELISA CCS, plasma, serum: 100 μL 22 pg/mL 22–6,000 pg/mL EHIL2RB

Human

Mouse

Coated ELISA CCS, plasma, serum: 50 µL 9.1 pg/mL 18.8–1,200 pg/mL BMS 221-2

Instant ELISA

HS Coated

ELISA

ProQuantum

ProcartaPlex

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.10 pg/mL 1.2–5,000 pg/mL EPX01A-20601-901

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 5.3 pg/mL 15.6 –1,0 0 0 p g/mL BMS601

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A42892

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.9 pg/mL 4.9–20,000 pg/mL EPX01A-36052-901

Coated ELISA CCS, serum: 50 µL 11 pg/mL 62.5 –2,000 pg/mL BMS634

Coated ELISA CCS, plasma, serum: 50 µL 0.27 ng/mL 0.31–20 ng/mL BMS2 12-2

Instant ELISA CCS, plasma, serum, urine: 10 µL 0.21 ng/mL 0.31–20 ng/mL BMS212INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 14 pg/mL 122.07–500,000 pg/mL E PX0 1A-10212- 90 1

Coated ELISA CCS, plasma, serum: 100 µL <1 pg/mL 7.8–500 pg/mL KHC0031

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35586

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 10.1 pg/mL 25.76–105,500 pg/mL EPX0 1A-1212 3-9 01

Coated ELISA CCS, plasma, serum: 100 μL 0.2 pg/mL 0.2–40 pg/mL EMIL3

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.11 pg/mL 0.4–1,700 pg/mL EPX01A-26035-901

Serum, plasma: 25 µL; CCS: 50 µL 0.199 pg/mL 0.44–1,820 pg/mL

CCS, plasma, serum: 100 µL 2 pg/mL 2–250 pg/mL 88-70 25

Amniotic ﬂuid, CCS, plasma, serum, synovial ﬂuid urine: 50 µL

CCS, plasma, serum: 50 µL 0.4 pg/mL 0.94–60 pg/mL BMS221HS

CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35603

Serum, plasma: 25 µL; CCS: 50 µL 0.168 pg/mL 0.12–490 pg/mL

CCS, plasma, serum: 100 µL 2 pg/mL 2–200 pg/mL 88-70 24

CCS, plasma, serum: 50 µL 0.05 pg/mL 0.78–50 pg/mL BMS601HS

CCS, plasma, serum, urine, amniotic ﬂuid, whole blood: 50 µL

2.3 pg/mL 18.8–1,200 pg/mL BMS221INST

9.1 pg/mL 18.8–1,200 pg/mL BMS64 3-2

EPXS010-10 221901

EPXS010-20601901

Target Species Format Volum e Sensitivity Range Cat. No.

Uncoated

ELISA

ProcartaPlex

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.5 pg/mL 12.21–50,000 pg/mL EPX01A-102 25 -901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 1.3 pg/mL 7.8–500 pg/mL BMS225-2

Instant ELISA

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35587

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.03 pg/mL 1.2–5,000 pg/mL EPX01A-20613-901

ProcartaPlex

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 2 pg/mL 3.9–250 pg/mL BM S613

Instant ELISA CCS, serum: 50 µL 0.6 pg/mL 3.9–250 pg/mL BMS613INST

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 μL <0.3 pg/mL 0.32–5,000 pg/mL A42893

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.2–5,000 pg/mL EPX01A-30613-901

Coated ELISA CCS, serum: 50 µL 0.2 pg/mL 1.6–100 pg/mL BMS628

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 7.32–30,000 pg/mL E PX0 1A-10278 -901

ProcartaPlex

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 1.5 pg/mL 7.8–500 pg/mL BMS278

Instant ELISA CCS, plasma, serum: 50 µL 1.45 pg/mL 7.8–500 pg/mL BMS278INST

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35588

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.32 pg/mL 2.4–10,000 pg/mL EPX01A-20610-901

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 3.3 pg/mL 7.8–500 pg/mL BM S610

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–5,000 pg/mL A44836

CCS, serum: 100 µL <15.6 pg/mL 15.6–1,000 pg /mL ESS0 031

Serum, plasma: 25 µL; CCS: 50 µL 0.237 pg/mL 0.86–3,530 pg/mL

CCS, plasma, serum: 100 µL 2 pg/mL 2–200 pg/mL 88-70 46

Amniotic ﬂuid, CCS, plasma, serum, synovial ﬂuid urine: 50 µL

CCS, plasma, serum: 50 µL 0.1 pg/mL 0.25–16 pg/mL BMS225HS

Serum, plasma: 25 µL; CCS: 50 µL 0.055 pg/mL 0.14–560 pg/mL

CCS, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-70 44

CCS, plasma, serum: 50 µL 0.32 pg/mL 0.63–40 pg/mL BMS613HS

Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 0.664–2,720 pg/mL

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-70 56

CCS, lavage ﬂuid, serum: 100 µL 4 pg/mL 4–500 pg/mL 88 -7054

0.66 pg/mL 7.8–500 pg/mL BMS225INST

EPXS010-10 225901

EPX S0 10-20 613 901

EPXS010-10 278 901

I L- 4

I L- 5

I L- 5 R A ( IL- 5 r e ce pt or subunit alpha; CD125)

Bovine

Human

Mouse

Rat

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.8 pg/mL 8.76–35,900 pg/mL EPX01A-40225-901 Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.072 pg/mL 1.44–5,900 pg/mL EPX01A-66050 -901

Human

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 2.4–10,000 pg/mL EPX01A-30610-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 10.35–42,400 pg/mL EPX01A-40278-901

Human Coated ELISA CCS, plasma, serum: 100 μL 150 pg/mL 0.15 – 40 ng /mL EHIL5RA

Over 4,500 references—visit our kit product pages or contact our Technical Support team at techsupport@thermoﬁsher.com for informative and valuable citations.

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

Bovine

Canine Coated ELISA CCS, plasma, serum: 100 μL 0.1 n g /mL 0.1– 2 5 n g /mL ECIL6 Porcine Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–10,000 pg/mL ESIL6

Rat

Human

I L- 6

Mouse

Rat

Monkey

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 4.028–16,500 pg/mL EPX01A-40213-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 12.13–49,700 pg/mL EPX01A-50505-901 Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.428 pg/mL 7.6–31,400 pg/mL EPX01A-66051-901

I L- 6 R ( C D126 ) Human

I L- 6 RA ( I L- 6 r e ce pt o r subunit alpha)

IL-6ST (gp130; IL-6 receptor subunit beta)

I L-7

IL-7R alpha Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.2 pg/mL 8–32,800 pg/mL EPX01A-26093 -901

Mouse Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–1,500 pg/mL EMIL6RA

Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–6,000 pg/mL EHIL6ST Mouse Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40 –10,000 pg/mL EMIL6ST Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–1,000 pg/mL EHIL7 Mouse Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10– 6,000 pg/mL EMIL7 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.61–2,500 pg/mL E PX01B-10237-901 Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 7.59–31,100 pg/mL EPX01A-26094-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.89–3,650 pg/mL EPX01A-40237-901 Human ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A35589

Uncoated

ELISA

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 9.77–40,000 pg/mL EPX01A -10213- 90 1

ProcartaPlex

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 0.92 pg/mL 1.56–100 pg/mL BMS213 -2

Instant ELISA CCS, serum: 50 µL 0.92 pg/mL 3.1–200 pg/mL BMS213INST

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 µL <0.05 pg/mL 0.064–10,000 pg/mL A35573

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.21 pg/mL 4.9 –20,000 pg/mL EPX01A-20603-901

ProcartaPlex

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 6.5 pg/mL 31.3–2,000 pg/mL BMS603-2

HS Coated

ELISA

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–5,000 pg/mL A43656

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.9 pg/mL 4.9–20,000 pg/mL EPX01A-36053-901

Coated ELISA CCS, serum: 50 µL 12 pg/mL 31–2,000 pg/mL BMS625

Coated ELISA CCS, serum: 50 µL 1.3 U/mL 1.9 –12 0 U /m L BMS641-2

Instant ELISA CCS, serum: 50 µL 1. 05 U/mL 1. 9 –120 U/mL BMS641INST

Coated ELISA

Instant ELISA

CCS, serum: 100 µL <78.1 pg/mL 78–5,000 pg/mL ESS0029

CCS, plasma, serum: 100 µL 31.2 pg/mL 31.2–2,000 pg/mL CRC00 63

Serum, plasma: 25 µL; CCS: 50 µL 0.038 pg/mL 1.03–4,230 pg/mL

CCS, plasma, serum: 100 µL 2 pg/mL 2–200 pg/mL 88-70 66

CCS, plasma, serum: 50 µL 0.03 pg/mL 0.8–5 pg/mL BMS213HS

Serum, plasma: 25 µL; CCS: 50 µL 0.066 pg/mL 0.70–2,850 pg/mL

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-70 64

CCS, plasma, serum: 50 µL 0.21 pg/mL 1.56–100 pg/mL BMS603HS

Amniotic ﬂuid, CCS, urine: 20 µL; plasma, serum: 10 µL

Amniotic ﬂuid, CCS, plasma, serum, urine: 10 µL

0.01 ng/mL 0.8–5 ng/mL BM S214

0.01 ng/mL 78–5,000 pg/mL BMS214INST

EPXS010-10 213 901

EPXS010-2060 3901

Over 4,500 references—visit our kit product pages or contact our Technical Support team at techsupport@thermoﬁsher.com for informative and valuable citations.

Target Species Format Volum e Sensitivity Range Cat. No.

Canine

Feline Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25– 6,000 pg/mL EFCXCL8

Human

IL-8 (CXCL8)

Monkey

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.2 pg/mL 3.19–13,100 p g/mL EPX01A-40204-901

Porcine

Human

I L- 9

Mouse

Canine Coated ELISA CCS, plasma, serum: 100 μL 0.1 n g /mL 0.1– 80 n g /mL ECI L10 Equine Coated ELISA CCS, plasma, serum: 100 μL 90 pg/mL 0.09–25 ng/mL E EIL10

Porcine

Human

I L-1 0

Mouse

Rat

Monkey

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 0.75–3,100 pg/mL EPX01A-40642-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.7 pg/mL 8.59–35,200 pg/mL EPX01A-50502-901 Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.25 pg/mL 3.75 –15,40 0 p g/mL EPX01A-66049 -901

IL-10Ra (CD210a) Human Coated ELISA CCS, plasma, serum: 100 μL 2.5 ng/mL 2.5–500 ng/mL EHI L10 RA I L-1 0 Rb Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–3,000 pg/mL EH IL10R B

I L-11

Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–800 pg/mL EH I L11 Mouse Coated ELISA CCS, plasma, serum: 100 μL 1,000 pg/mL 1–450 ng/mL E MIL11

Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–1,500 pg/mL ECCXCL 8

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.9 pg/mL 10.08–41,300 pg/mL EPX01A-50506-901

HS Coated

ELISA

ProcartaPlex

ProQuantum CCS, plasma, serum: 2–5 µL <0.01 pg/mL 0.0128–5,000 pg/mL A 35575

Uncoated

ELISA

Coated ELISA

Instant ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.2 pg/mL 2.44–10,000 pg/mL E PX0 1A-1020 4- 901

Coated ELISA CCS, serum: 50 µL 3.0 U/mL 19.5–1,250 U/ mL B MS640- 3

Instant ELISA

Coated ELISA Serum: 50 μL; CCS: 100 μL <10 pg/mL 31.2–2,000 pg/mL KSC0081

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.43 pg/mL 15.77–64,600 pg/mL EPX01A-66052-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.5 pg/mL 9.77–40,000 pg/mL EPX01A-12081-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 100 µL 0.5 pg/mL 3.1–200 pg/mL BMS2081

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.28 pg/mL 18.3–75,000 pg/mL EPX01A-26041-901

Uncoated

ELISA

Coated ELISA

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 2.44–10,000 pg/mL EPX0 1A-10215 -9 01

ProcartaPlex

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 1 pg/mL 3.15–200 pg/mL B MS 215 -2

Instant ELISA CCS, plasma, serum: 50 µL 0.66 pg/mL 3.1–200 pg/mL BMS215INST

HS Coated

ELISA

ProQuantum CCS, serum, plasma: 2–5 µL <0.05 pg/mL 0.064– 5,000 pg/mL A35590

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.69 pg/mL 2.4–10,000 pg/mL EPX01A-20614-901

Uncoated

ELISA

Instant ELISA CCS, plasma, serum: 50 µL 5.28 pg/mL 39.1–2,500 pg/mL BMS614INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.6 pg/mL 9.8–40,000 pg/mL EPX01A-36049-901

Coated ELISA CCS, serum: 50 µL 1.5 pg/mL 15.6 –1,0 00 pg/mL BMS629

Coated ELISA CCS, serum: 50 µL 1.3 U/mL 3.9–250 U/mL BMS642-2

Instant ELISA CCS, plasma, serum: 50 µL 1. 32 U/ mL 3.9–250 U/mL BMS642INST

CCS, plasma, serum: 100 µL <0.1 pg/mL 0.39–25 pg/mL KHC008 4

Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 0.217–890 pg/mL

CCS, plasma, serum: 100 µL 2 pg/mL 2–250 pg/mL 88-8086

Amniotic ﬂuid, CCS, plasma, serum: 50 µL

CCS, plasma, serum: 50 µL 1 pg/mL 1–100 pg/mL 88-795 8

CCS, serum: 100 µL 32 pg/mL 32–4,000 pg/mL 88-8092

Serum, plasma: 50 μL; CCS: 100 μL

CCS, plasma, serum: 100 µL 31.2 pg/mL 31.2–2,000 pg/mL CSC 0103

Serum, plasma: 25 µL; CCS: 50 µL 0.038 pg/mL 0.18–750 pg/mL

CCS, plasma, serum: 100 µL 2 pg/mL 2–300 pg/mL 88-7 106

CCS, plasma, serum: 50 µL 0.05 pg/mL 0.39–25 pg/mL BMS215HS

CCS, serum: 100 µL 32 pg/mL 32–4,000 pg/mL 88 -710 5

2 pg/mL 15 .6 –1,0 00 pg/mL BMS204-3

1.3 pg/mL 15.6–1,000 pg /m L BMS204-3INST

1.5 U/mL 19.5–1,250 U/mL BMS640-3INST

<3 pg/mL 7.8–500 pg/mL KSC0101

EPXS010-10 20 4901

EPXS010-10 215 901

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 1.81–7,400 pg/mL EPX01A-12090-901 Human

I L-1 2 / IL- 2 3 p4 0

I L-1 2 p70

I L-1 2 ( I L-12 A) Porcine Coated ELISA CCS, plasma, serum: 100 μL 2.2 ng/mL 2.2–600 ng/mL ES IL12 A IL-12 p40/70 (IL-12B) Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–2,000 pg/mL E M IL12B I L-1 2 ( t o ta l) Rat Coated ELISA CCS: 100 µL; plasma, serum: 10 µL <3 pg/mL 15.6 –1,000 pg/mL KRC0121

I L-1 3

IL-13R alpha 2 Human Coated ELISA CCS, plasma, serum: 100 μL 0.4 ng/mL 0.4–100 ng/mL EHI L13 R A2

I L-1 5

I L-1 5 / IL-15 R Mouse

I L-1 6 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.7 pg/mL 15.45– 63,300 pg/mL EPX01A -12162 -9 01

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 4.05–16,600 pg/mL EPX01A-36050-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 1.81–7,400 pg/mL EPX01A-42090-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.8 pg/mL 6.62–27,100 pg/mL EPX01A-50503-901 Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.46 pg/mL 45.14–184,900 pg/mL EPX01A-62090-901 Rat Coated ELISA CCS, plasma, serum: 100 µL <2.5 pg/mL 7.8–500 pg/mL KRC2371

Human

Mouse

Monkey Coated ELISA CCS, serum: 50 µL 2.6 U/mL 3.9–250 U/mL BMS646 Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.9 pg/mL 9.8–40,000 pg/mL EPX01A-36004-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.04 pg/mL 1.5–6,150 pg/mL EPX01A-40238-901

Porcine Coated ELISA CCS, plasma, serum: 100 μL 16 pg/mL 16 –15,000 pg /mL ESI L13 Rat Coated ELISA Tissue lysates, cell lysates: 100 μL 20 pg/mL 20–20,000 pg/mL E R IL13

Human

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 4.9–20,000 pg/mL EPX01A-36015-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.57–6,450 pg/mL EPX01A-40231-901

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 3.54–14,500 pg/mL EPX01A-42089-901

Coated ELISA CCS, serum, plasma: 50 µL <10 pg/mL 31.3–2,000 pg/mL BMS2013

ProQuantum CCS, plasma, serum: 2–5 µL <0.05 pg/mL 0.064–10,000 pg/mL A35577

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 4 pg/mL 31.3–2,000 pg/mL BMS 616

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.5 pg/mL 4–16,200 pg/mL EPX01A-26033-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.04 pg/mL 6.84–28,000 pg/mL E PX01A-10 23 8- 901

ProcartaPlex

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 2.1 pg/mL 3.1–200 pg/mL BMS238

HS Coated

ELISA

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35591

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.21 pg/mL 2.4–10,000 pg/mL EPX01A-26004-901

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 10.0 pg/mL 15.6 –1,0 00 pg/mL BMS6004

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 2.44–10,000 pg/mL EPX0 1A-10231-901

Uncoated

ELISA

Coated ELISA

Instant ELISA CCS, plasma, serum: 50 µL 0.99 pg/mL 3.13–200 pg/mL BMS231INST

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A35609

Coated ELISA CCS, plasma, serum: 50 µL 2.8 pg/mL 7.8–500 pg/mL BMS60 15

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.16 pg/mL 2.4–10,000 pg/mL EPX01A-26015-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 3.05–12,500 pg/mL EPX01A-12089-901

Coated ELISA CCS, plasma, serum: 50 µL 3.4 pg/mL 15.6 –1,0 0 0 p g/mL B MS2106

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.42 pg/mL 2.0–8,050 pg/mL EPX01A-26023-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 2 pg/mL 6.3–400 pg/mL BMS6023

CCS, serum: 100 µL 2 pg/mL 2–300 pg/mL 8 8-712 0

Serum, plasma: 25 µL; CCS: 50 µL 0.044 pg/mL 0.75–3,090 pg/mL

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 8 8-712 6

CCS, plasma, serum: 50 µL 0.1 pg/mL 0.16–10 pg/mL BMS238HS

CCS, serum: 100 µL 15 pg/mL 15–2,000 pg/mL 88 -7 121

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-7439

Amniotic ﬂuid, CCS, plasma, serum: 50 µL

CCS, plasma, serum: 100 µL 20 pg/mL 20–2,500 pg/mL 88 -762 0

CCS, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-7215

2.8 pg/mL 7.8–500 pg/mL BMS231-3

EPXS010-10 23 8901

Target Species Format Volum e Sensitivity Range Cat. No.

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 0.5 pg/mL 1.6–100 pg/mL BM S20 17

HS Coated Human

I L-1 7A ( IL-17/CT L A- 8)

Mouse

Rat

Porcine Coated ELISA CCS, plasma, serum: 100 μL 14 pg/mL 14–4,000 pg/mL ESI L17A NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.4 pg/mL 6.98–28,600 pg/mL EPX01A-42017-901

I L-1 7A F

I L-1 7 B

I L-1 7 E ( I L-2 5 ) Mouse

I L-1 7 F

I L-1 7 R Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–3,000 pg/mL E HIL17 R

I L-1 8

IL-18BP (IL-18 binding protein)

IL-18RB (I L18R AP ) Human Coated ELISA CCS, plasma, serum: 100 μL 12 pg/mL 12–3,000 pg/mL E HI L18 R AP

I L-1 9

I L- 2 0

Human

Mouse

Human Coated ELISA CCS, plasma, serum: 100 μL 0.3 ng/mL 0.3 –10 0 n g/mL EH IL17B Mouse Coated ELISA CCS, plasma, serum: 100 μL 12 pg/mL 12–12,00 0 pg /m L E MIL17B

Human

Mouse

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 2.05–8,400 pg/mL EPX01A-42160-901

Rat Coated ELISA CCS, plasma, serum: 100 µL <4 pg/mL 15.6–1,00 0 pg/mL KRC 2341

Human

Mouse

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 1.95–8,000 pg/mL EPX01A-40267-901 Porcine Coated ELISA CCS, serum: 50 µL 23 pg/mL 39.1–2,500 pg/mL BMS672

Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–18,000 pg/mL EHIL18BP

Mouse ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 3 2.6 –13 3,50 0 p g/mL EPX01A-26048-901

Mouse

Human Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–500 pg/mL EHIL20 Mouse Coated ELISA CCS, plasma, serum: 100 μL 16 pg/mL 16–4,000 pg/mL EMIL20 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.6 pg/mL 12.26–50,200 pg/mL EPX01A-12168 -9 01

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 2.44–10,000 pg/mL EPX01A-12017-901

ProcartaPlex

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–25,000 pg/mL A35 6 11

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 1.6 pg/mL 7.8–500 pg/mL BMS 6001

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.08 pg/mL 1.2–5,000 pg/mL EPX01A-26001-901

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–25,000 pg/mL A46737

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 1 pg/mL 1.6–100 pg/mL BMS635

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 3 .7–15,00 0 pg/mL EPX01A-30635-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.55 pg/mL 6.1–25,000 pg/mL EPX01A-12082-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 8.8 pg/mL 31.3–2,000 pg/mL BMS2082

Uncoated

ELISA

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 0.9 pg/mL 3.9–250 pg/mL BMS6046

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 2.05–8,400 pg/mL E PX01A-12160 -9 01

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 15.5 pg/mL 62.5–4,000 pg/mL BMS20 37- 2

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A 356 12

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.13 pg/mL 2.4–10,000 pg/mL EPX01A-26020-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 10 pg/mL 62.5–4,000 pg/mL BMS6020

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 9.77–40,000 pg/mL EPX01A -102 67-901

Coated ELISA CCS, plasma, serum: 50 µL 9 pg/mL 78–5,000 pg/mL BMS267-2

Instant ELISA CCS, plasma, serum, urine: 50 µL 9.2 pg/mL 78–5,000 pg/mL BMS267INST

ProQuantum CCS, serum, plasma: 2–5 µL <0.05 pg/mL 0.064–1,000 pg/mL A35613

Coated ELISA CCS, plasma, serum: 50 µL 10 pg/mL 31.3–2,000 pg/mL BMS 618 -3

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 9.95 pg/mL 3 6.6–150,000 pg /m L EPX01A -20618- 90 1

Uncoated

ELISA

CCS, serum: 100 µL 4 pg/mL 4–500 pg/mL 88 -7176

CCS, plasma, serum: 50 µL 0.01 pg/mL 0.23–15 pg /m L BMS2017HS

Serum, plasma: 25 µL; CCS: 50 µL 0.016 pg/mL 0.25–1,030 pg/mL

CCS, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-7371

CCS, serum: 100 µL 2 pg/mL 2–200 pg/mL 8 8 -7170

CCS, plasma, serum: 100 µL 30 pg/mL 30–4,000 pg/mL 88-7117

CCS, serum: 50 µL 30 pg/mL 30–4,000 pg/mL 88-8711

CCS: 100 µL; Serum: 12.5 µL 16 pg/mL 16–2,000 pg/mL 88-70 02

CCS, plasma, serum: 100 µL 16 pg/mL 16–2,000 pg/mL 88-7478

CCS, serum: 100 µL 15 pg/mL 15–2,000 pg/mL 88- 7472

CCS, plasma, serum: 10 µL 62.5 pg/mL 62.5–4,000 pg/mL 8 8-50320

EPXS010-12017901

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 9.77–40,000 pg/mL EPX01A-12043-901

Uncoated Human

I L- 21

Mouse

Rat ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.3 pg/mL 1.71–7,000 pg/mL EPX010-36021-901

Human

I L- 2 2

Mouse

Human

IL-23 (heterodimer) (IL12B/IL23A)

Mouse

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.9 pg/mL 6.71–27,500 pg/mL EPX01A-42023-901

I L- 2 5 ( I L-17 E ) Mouse ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 4.6–19,100 pg/mL EPX01A-26046-901

Human

IL-27

Mouse

Human

IL-28A (IFN lambda 2)

Mouse

I L- 2 8 A Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–10,0 00 pg/mL EHIL28A

IL-28B (IFN lambda 3) Mouse

IL-29 (IFN lambda 1) Human

Human

IL-31

Mouse

Human

IL-33

Mouse

IL-33R (ST2) Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 66.7 pg/mL 122.07–500,000 pg/mL EPX010-12231- 901 IL-34 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.5 pg/mL 10–40,000 pg/mL EPX010 -123 42-9 01 IL-36G (IL-1F9) Human Coated ELISA CCS, plasma, serum: 100 μL 13 pg/mL 13–3,000 pg/mL EHIL36G

IL-37 (FIL1/IL-1F7) Human

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 20 pg/mL 78–5,000 pg/mL BMS20 43

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–25,000 pg/mL A35593

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 5 pg/mL 16.1–1,000 pg/mL BMS6021

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.5 pg/mL 8.81–36,100 pg/mL EPX01A-26021-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 8.2 pg/mL 31.74–130,0 00 pg/mL EPX01B-12047-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 5 pg/mL 31.3–2,000 pg/mL BMS2 047

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 5 pg/mL 7.8–500 pg/mL BMS6022

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.24 pg/mL 12.2–50,000 pg/mL EPX01A-26022-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.9 pg/mL 14.65–60,000 pg/mL EPX01A-12023-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 4 pg/mL 15.6–2,000 pg/mL BMS2023-3

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.21 pg/mL 12.2–50,000 pg/mL EPX01A-26017-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 16 pg/mL 31.3–2,000 pg/mL BMS6 017

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.1 pg/mL 24.41–100,000 pg/mL EPX01A-12085-901

Coated ELISA CCS, plasma, serum: 50 µL 9.5 pg/mL 62.5–4,000 pg/mL BMS2085

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 8.76 pg/mL 31.3–2,000 pg/mL BMS6 024

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.34 pg/mL 2.4–10,000 pg/mL EPX01A-26024-901

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 20.31 pg/mL 41–168,000 pg/mL EPX01A-26028-9 01

Coated ELISA CCS, plasma, serum: 50 µL 12.85 pg/mL 31.3–2,000 pg/mL BMS6028

Uncoated

ELISA

Uncoated

ELISA

Coated ELISA CCS, serum, plasma: 50 µL 2 pg/mL 15.6 –1,0 00 pg/mL BMS2049

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 28.3 pg/mL 51.83–212,300 pg/mL EPX01A-12049-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.3 pg/mL 19.53–80,000 pg/mL EPX01A-12041-901

Coated ELISA CCS, plasma, serum: 50 µL 1 pg/mL 7.8–500 pg/mL BM S2041

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.45 pg/mL 7.6–31,200 pg/mL EPX01A-26030-901

Coated ELISA CCS, serum, plasma: 50 µL 9.1 pg/mL 15.65–1,000 pg/mL BMS6030

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.24 pg/mL 2.29–9,400 pg/mL EPX01A-12048-901

Coated ELISA CCS, serum, plasma: 50 µL 0.2 pg/mL 7.8–500 pg/mL BMS2048

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 25.5 pg/mL 21.75–89,100 pg/mL EPX010-26025-901

Coated ELISA CCS, serum, plasma: 50 µL 11.2 pg/mL 46.9–3,000 pg/mL BMS6025

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1.99 pg/mL 4.22–17,300 pg/mL EPX010-1226 8-9 01

Uncoated

ELISA

CCS, plasma, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 88-8218

CCS, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 88-8210

CCS, plasma, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 8 8-7522

CCS, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 8 8 -74 22

CCS, plasma, serum: 100 µL 15 pg/mL 15–2,000 pg/mL 88 -7237

CCS, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 8 8-7230

CCS, Serum: 100 µL 16 pg/mL 16–2,000 pg/mL 88- 72 74

CCS, plasma, serum: 50 µL 15.6 pg/mL 15.6 –1,000 pg/mL 88- 52102

CCS, serum: 100 µL 32 pg/mL 32–4,000 pg/mL 88 -728 4

CCS, plasma, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 8 8-7296

CCS, serum: 100 µL 25 pg/mL 25–3,000 pg/mL 88-7333

CCS, plasma, serum: 50 µL 31.3 pg/mL 31.3–2,000 pg/mL 8 8-52103

Target Species Format Volum e Sensitivity Range Cat. No.

Human Coated ELISA Serum: 50 μL 0.17 U /mL 5.1– 250 U/ m L K AQ 1251

Insulin

IP-10 (CXC L10/CRG-2)

Iso-insulin Human Instant ELISA CCS, plasma, serum: 50 μL 16.6 pg/mL 31.3–2,000 pg/mL BMS2003INST

I-TA C (CX C L11)

JNK 1/2 (total/phospho) Multispecies

JNK 1/2 [pThr183/ Tyr 18 5]

JNK 1/2 (total) Multispecies

JAK2 (total) Human Coated ELISA Cell lysate: 10 µL <0.95 ng/mL 0.95–60 ng/mL KHO5521 JA K 2 [pYpY10 07/1008 ] Human Coated ELISA Cell lysate: 10 µL 1.5 U/mL 0–100 U/mL KHO5621 Kallikrein-6 (KLK6) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.6 pg/mL 6–22,800 pg/mL EPX010 -123 55 -901 KLK-10 Human Coated ELISA CCS, plasma, serum: 100 μL 2 ng/mL 2– 600 ng/mL EHKLK10 KL K14 Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–500 pg/mL EH KLK14 KLK6 Human Coated ELISA CCS, plasma, serum: 100 μL 0.6 ng/mL 0.6 –150 ng/mL EHKLK6

KRT18 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 698.9 pg/mL

Lactoferrin Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 324.02 pg/mL 94.29–386,200 pg/mL E PX0 10-1225 0-901

LAG-3 (CD223)

LAP (TGF beta 1)

LDH-B Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1,775.02 pg/mL

Leptin

Leukotriene B4 (LTB4) Multispecies Coated ELISA

Levonorgestrel (LNG) Multispecies

LH (lutenizing hormone) Human Coated ELISA CCS, plasma, serum: 100 μL 140 pg/mL 140–10,000 pg/mL EHLH

LIF

LIGHT (CD258)

Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 µIU/mL 5–400 μlU/mL EMINS Rat Coated ELISA CCS, plasma, serum: 100 μL 5 μlU/mL 5–300 μlU/mL ERINS Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 195.31–800,000 pg/mL EPX010-12 00 3-901 Human Coated ELISA CCS, saliva, serum, plasma: 50 µL <2 pg/mL 7.8–500 pg/mL KAC2361

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 1.95–8,000 pg/mL EPX01A-10284 -9 01

Human

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.9 pg/mL 4.9–20,100 pg/mL EPX01A-30636-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 10.54–43,200 pg/mL EPX01A-40284-901

Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–600 pg/mL EHCXCL11 Mouse Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–4,000 pg/mL EMCXCL11 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.6 pg/mL 5.37–22,000 pg/mL E PX01A-1212 4-9 01 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.8 pg/mL 5.63–23,100 pg/mL EPX01A-42124-901

Multispecies

Human

Mouse ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 72.6 pg/mL 73–298,900 pg/mL EPX010-26051-901

Human

Mouse

Human Coated ELISA Plasma, serum: 10 µL; CCS: 100 µL <3.5 pg/mL 15.6–1,00 0 pg/mL KAC2281 Mouse Coated ELISA Plasma, serum: 10 µL; CCS: 100 µL <50 pg/mL 93.8–6,000 pg/mL KMC2281 Rat Coated ELISA Plasma, serum: 20 µL; CCS: 100 µL <20 pg/mL 62.5–4,000 pg/mL KRC2281

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 7.15–29,300 pg/mL EPX01A-26036-901 Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 16.92–69,300 pg/mL EPX01A-32039 -901

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.28 pg/mL 2.4–9,850 pg/mL EPX01A-26040-901 Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–6,000 pg/mL EHT NF SF14 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.31 pg/mL 2.09–8,550 pg/mL EPX01A-12009-901

Instant ELISA

ProQuantum CCS, plasma, serum: 2–5 µL <0.05 pg/mL 0.064–10,000 pg/mL A35578

Coated ELISA CCS, plasma, serum: 50 µL 6.5 pg/mL 7.8–500 pg/mL BMS6018

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.26 pg/mL 0.5–2,000 pg/mL EPX01A-26018-901

InstantOne

ELISA

InstantOne

ELISA

InstantOne

ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 3.37 pg/mL 12.21–50,000 pg/mL EPX01A-12211-901

Coated ELISA CCS, plasma, serum: 10 µL 2.20 pg/mL 6.2–400 pg/mL BM S22 11

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 10 µL 0.098 ng/mL 0.16 –10 n g / m L BMS2065

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.96 ng/mL 3.052–12,500 pg/mL E PX0 10-12065 -9 01

ProQuantum CCS, serum, plasma: 2–5 µL <15 pg/mL 16–250,000 pg/mL A45730

Uncoated

ELISA

ProQuantum CCS, serum, plasma: 2–5 µL <0.03 ng/mL 0.32–2,500 ng/mL A46342

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.0 pg/mL 19.31–79,100 pg/mL EPX01A-12039-901

Instant ELISA 50 µL (1:25 prediluted) 20.0 pg/mL 63–4,000 pg/mL BMS2039INST

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–25,000 pg/mL A45250

Competitive

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.83 pg/mL 6.1–25,000 pg/mL EPX01A-10 242-9 01

Coated ELISA CCS, plasma, serum: 50 µL 0.7 pg/mL 3.15–200 pg/mL BMS242

Amniotic ﬂuid, CCS, serum, synovial ﬂuid, plasma, urine: 10 µL

Cell lysate: 20 µL 85 -86198

Cell lysate: 20 µL 85 -86197

Cell lysate: 20 µL 85 -86196

CCS, plasma, serum: 10 µL 156.3 pg/mL 156.3–10,00 0 p g/mL 88-50390

CCS, plasma, serum: 10 µL 0.2 ng/mL 0.2–12.5 ng /m L 88-50690

CCS, urine: 100 µL; plasma: 50 µL; saliva: 25 µL

Serum, plasma, CCS: 100 μL 2.2 pg/mL 6.25–200 pg/mL EIALNGC

1 pg/mL 3.1–200 pg/mL BMS284INST

345.48 –1,415,100 pg/ mL

662.01–2,711,600 pg/mL

<19.4 pg/mL 47–12,000 pg/mL EHLT B4

EPX010 -12247-901

EPX010 -12262- 901

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

LIMP-II (SCARB2) Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–2,500 pg/mL EHSCARB2

Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–1,000 pg/mL EHLCN2

Lipocalin-2 (LCN2)

LOX-1 Human

Lp-PLA2 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1.665 pg/mL 12–50,000 pg/mL EPX010-12324 -901

L-selectin (CD62L)

Lymphotactin XCL1

LY VE-1 Human

mAB Isotyping Mouse Coated ELISA CCS, ascites 3 ng/mL 37503 Mast cell protease-1

(MCPT-1)

MBL (MBL2) Human

MBL oligomer Human Coated ELISA Plasma, serum: 10 µL < 0 .1 ng/mL 0.4–40 ng/mL KIT029

MCP-1 (CCL2)

MCP-2 (CCL8) Human

MCP-3 (CCL7)

MCP-4 (CCL13) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.688 pg/mL 0.85–3,500 pg/mL EPX010-12378- 90 1 MCP-5 (CCL12) Mouse Instant ELISA CCS, plasma, serum: 50 µL 2.3 pg/mL 15.6 –1,0 0 0 p g/mL BMS6007INST

M-CSF

MDC (CCL22)

Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.02 ng/mL 0.02 –100 ng/mL EMLCN2 Rat Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–8,000 pg/mL ER LCN2

Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–500 pg/mL EHOLR1

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.7 pg/mL 1.2–5,100 pg/mL E PX010-1229 6-901

Mouse Coated ELISA CCS, plasma, serum: 100 μL 35 pg/mL 35 –10,000 pg/mL EMSELL Rat Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–6,000 pg/mL ERSELL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 10.5 pg/mL 6.62–150,0 00 pg/mL EPX01A-10206- 901 Human

Human Coated ELISA CCS, plasma, serum: 100 μL 150 pg/mL 0.15 – 50 ng /mL EHXCL1 Mouse Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40 –3,000 pg/mL EMXCL1

Mouse

Human

Mouse

Rat

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 3.76 –15,400 pg/mL EPX01B-40281-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.7 pg/mL 6.23–25,500 pg/mL EPX01A-50507-901

Human

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 3.5–14,200 pg/mL EPX01A-36006-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 3 .9 –15,800 pg /m L EPX01A-42125-901

Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–2,000 pg/mL EHCSF1 Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–350 pg/mL EMCSF1 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 7 pg/mL 16.72–68,500 pg/mL E PX01A -1212 6 -9 01 Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.02 pg/mL 0.3–1,100 pg/mL EPX01A-26039-901 Mouse Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–600 pg/mL EMCCL22

Human

Coated ELISA 50 µL (1:100 prediluted) 0.2 ng/mL 0.4–25 ng/mL BMS206

Instant ELISA 50 µL (1:100 prediluted) 0.19 ng /mL 0.39–25 ng/mL BMS206INST

Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–2,000 pg/mL E H LY V E1

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 6.31 pg/mL 10.60–43,400 pg/mL EPX010-12239-9 01

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 100 μL 0.03 ng/mL 0.03–25 ng/mL EHMBL2

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 52.81 pg/mL 71.22–291,700 pg/mL EPX 010 -12284 -9 01

Uncoated

ELISA

Coated ELISA

Instant ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 1.22–5,000 pg/mL E PX01B-10281-901

ProcartaPlex

High

Sensitivity

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A35598

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 2.2 pg/mL 15.6 –1,0 0 0 p g/mL BMS6005

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.43 pg/mL 7.3–30,000 pg/mL EPX01A-26005-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 10.9 pg/mL 18.3–75,000 pg/mL EPX01A-36005-901

Instant ELISA CCS, serum, plasma: 25 µL 4.7 pg/mL 7.8–500 pg/mL BMS631INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.49–2,000 pg/mL EPX01A-1216 9-9 01

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35599

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 3.9 –15,800 pg /m L EPX0 1A-1212 5-9 01

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 0.1 pg/mL 0.3–20 pg/mL BMS2125

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A35600

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.15 pg/mL 0.2–1,000 pg/mL EPX01A-26006 -901

Instant ELISA CCS, plasma, serum: 50 µL 2.6 pg/mL 15.6–1,000 pg /mL BMS6006INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.28 pg/mL 12.42–50,900 pg/mL EPX01A-102 82-9 01

ProQuantum CCS, plasma, serum: 2–5 μL <1.5 pg/mL 1.6–25,000 pg/mL A42895

CCS, plasma, serum: 50 μL (1:150 prediluted)

CCS, serum: 100 µL 120 pg/mL 120 –15,000 pg /m L 8 8-7503

CCS, plasma, serum: 100 µL 7 pg/mL 7–1,000 pg/mL 88-73 99

Amniotic ﬂuid, CCS, plasma, serum: 20 µL

Serum, plasma: 25 µL; CCS: 50 µL 0.7 pg/mL 0.159–650 pg/mL

CCS, plasma, serum: 100 µL 15 pg/mL 15–2,000 pg/mL 88 -7391

CCS, plasma, serum: 50 µL 0.3125 pg/mL 0.3125–20 pg/mL 88-50700

1.09 pg/mL 1.09–70.0 pg/mL 88-52239-22

2.3 pg/mL 15.6 –1,0 00 pg/mL BMS281

2.3 pg/mL 15.6 –1,0 00 pg/mL BMS281INST

EPXS010-10 281901

Target Species Format Volum e Sensitivity Range Cat. No.

Mer (MERTK) Human

MFG-E8 Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–600 pg/mL EMMFGE8

MICA Human

MICB Human

Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6 –6,000 pg/mL EHMIF

MIF

MIG (CXCL9)

MIP-1 alpha (CCL3)

MIP-1 beta (CCL4)

MIP-2 alpha (CXCL2)

MIP-3 beta (CCL19) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.426 pg/mL 10.89–44,600 pg/mL EPX010 -12380 -901

MIP-3 alpha (CCL20) Human

MMP -1

MMP-2 Human Coated ELISA CCS, plasma, serum: 50 μL <1 ng /mL 0.78–50 ng/mL KHC3082

MMP-3 Human

MM P-7 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.04 pg/mL 7.37–30,200 pg/mL EPX01A-12133-901

MMP-8

MMP-9 Human

MMP -10 Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–1,500 pg/mL EHMMP10 MMP -12 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.4 pg/mL 4.30–17,600 pg/mL EPX01A-12130-901

MMP -13

MSP (MST1) Human Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–2,000 pg/mL EHMST1 MPIF (CCL23) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 20.789 pg/mL 21.8–89,300 pg/mL EPX010 -12385 -9 01 Mucin 1 (CA15-3) Human Coated ELISA Plasma, serum: 3.3 µL; CCS: 100 µL 4 mU/mL 4.1–1,000 mU/mL EHMUC1 Mucin 16 (CA125) Human Coated ELISA CCS, plasma, serum: 100 μL 0.6 U/mL 0.6 –400 U/mL E HM UC 16

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.06 pg/mL 0.4–1,600 pg/mL E PX01A -1212 7-90 1 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.06 pg/mL 0.4–1,600 pg/mL EPX01A-42127-901 Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20– 6,000 pg/mL EHCXCL9

Mouse

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.36 pg/mL 6.12–25,100 pg/mL EPX01A-40285-901

Human

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 2.3–9,500 pg/mL EPX01A-36013-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 6.03–24,700 pg/mL EPX01A-42029-901

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.16 pg/mL 1.2–5,000 pg/mL EPX01A-26014-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.7 pg/mL 22.045–90,300 pg/mL EPX01A-42030-901 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.32 pg/mL 0.81–3,300 pg/mL EPX010 -123 82-901 Rat Coated ELISA Plasma, serum: 50 μL; CCS: 100 μL <1 pg/mL 10–640 pg/mL KRC1021

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 0.6–2,400 pg/mL EPX01A-36054-901

Human

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.04 pg/mL 7.84–32,100 pg/mL EPX01A-12 129 - 901

Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6 –6,000 pg/mL EHMMP8 Rat Coated ELISA CCS, plasma, serum: 100 μL 50 pg/mL 0.05–40 ng/mL ERMMP8 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 8 pg/mL 25.17–103,100 p g/mL EPX01A-12134-901

Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6 –6,000 pg/mL EHMMP13 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.1 pg/mL 5.76–23,600 pg/mL EPX01A-12131-901

Coated ELISA CCS, plasma, serum: 100 μL 0.15 ng/m L 0 .15 – 4 0 n g /mL EHMER

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 121.2 pg/mL 171–174,70 0 pg/m L EPX010 -12285 -9 01

Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–10,000 pg/mL EHMICA

Coated ELISA CCS, plasma, serum: 20 μL 1.09 pg/mL 1.6–100 pg/mL BMS2302

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 7.77 pg/mL 12.96– 53,100 pg/mL E PX0 10-12 30 2-901

Coated ELISA CCS, plasma, serum: 100 μL 75 pg/mL 0.075–50 ng/mL EHMICB

Coated ELISA CCS, plasma, serum: 50 μL 1.24 pg/mL 3–200 pg/mL BMS2303

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 6.55 pg/mL 7.32–30,000 pg/mL EPX010-12303- 90 1

Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–2,000 pg/mL EMCXCL9

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.7 pg/mL 16–63,600 pg/mL EPX010-26061-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.36 pg/mL 22.2–91,100 pg/mL EPX01A-10 28 5- 901

Instant ELISA CCS, serum: 50 µL 4 pg/mL 31.3–2,000 pg/ mL BMS285INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.1 pg/mL 2.14–8,750 pg/mL EPX01A-12029-901

Instant ELISA CCS, plasma, serum: 50 μL 6 pg/mL 15.6–1,00 0 pg /mL BMS2029INST

ProQuantum CCS, serum, plasma: 2–5 µL <0.6 pg/mL 0.64–10,000 pg/mL A35616

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.13 pg/mL 0.5–2,000 pg/mL EPX01A-26013-901

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.7 pg/mL 6.1–25,000 pg/mL EPX01A-12030-901

Instant ELISA CCS, plasma, serum: 50 μL 3 pg/mL 7.8–500 pg/mL BMS2030INST

ProQuantum CCS, plasma, serum: 2–5 μL <0.05 pg/mL 0.064– 5,000 pg/mL A35597

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.32 pg/mL 0.81–3,300 pg/mL EPX010-12382-901

Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–2,000 pg/mL EMCXCL2

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.37 pg/mL 0.7–3,000 pg/mL EPX01A-26032-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.2 pg/mL 1.95–8,000 pg/mL EP X01A-12 128- 901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5.426 pg/mL 10.89–44,600 pg/mL EPX010-1238 0- 901

Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–18,000 pg/mL EHMMP1

Coated ELISA Tissue lysates, cell lysates: 100 μL 8 pg/mL 8–18,000 pg/mL EHMMP1CL

Coated ELISA CCS, plasma, serum: 10 μL 0.0005 ng/mL 0.06–4 ng/mL B MS2 014 - 3

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.6 pg/mL 17.97–73,600 pg/mL EPX01A-12014-901

Coated ELISA CCS, plasma, serum: 10 μL 0.05 ng/mL 0.23–15 ng /m L BMS 2016-2

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.83–3,400 pg/mL EPX01A-12016-901

CCS, plasma, serum: 50 μL 0.8 pg/mL 0.8–50 pg/mL 88 - 56 013

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

Mouse Coated ELISA CCS, plasma, serum: 100 μL 600 pg/mL 0.6 –150 ng/mL EMMPO

Myeloperoxidase (MPO)

Myoglobin Human

N-CAM1 Human

Nectin-2 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 19.06 pg/mL 37.30 –152,800 pg /m L EPX010-12304- 90 1 Neprilysin (MME) Human Coated ELISA CCS, plasma, serum: 100 μL 0.4 ng/mL 0.4–100 ng/mL EHMME Neuroﬁlament heavy

(NF-H) Neurogranin (NRGN) Human ProcartaPlex Serum, plasma, CSF: 25 μL 5.6 pg/mL 10–41,600 pg/mL E PX0 10-12 352-901 NF kappaB p65 (total/

phospho) NF kappaB p65

[pSer536]

NF kappaB p65 (total) Human

NGAL

NGF beta (beta-NGF)

NGFR Human Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 80 pg/mL–20 ng/mL EHNGFR Nidogen-1 (NID1) Human Coated ELISA CCS, plasma, serum: 100 μL 100 pg/mL 0.1– 3 0 n g / m L EHNID1 Nitric oxide (total) Multispecies Coated ELISA Serum, plasma, urine, saliva: 50 µL 1. 35 μ M 3 .1 –10 0 μM EMSNOTOT

Nitric oxide Multispecies Coated ELISA Serum, plasma, urine, saliva: 50 µL

NOTCH -1 Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–7,000 pg/mL EHNOTCH1 NOV Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–30 ng/mL EHNOV NPPB (BNP) Human Coated ELISA CCS, plasma, serum: 100 μL 14 pg/mL 14–1,000 pg/mL EHNPPB NrCAM Human Coated ELISA CCS, plasma, serum: 100 μL 0.3 ng/mL 0.3–80 ng/mL EHNRCAM NRG1 beta-1 (NRG1) Human Coated ELISA CCS, plasma, serum: 100 μL 50 pg/mL 50–20,000 pg/mL EHNRG1 NRP -1 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 148.39 pg/mL 86.57–354,600 pg/mL EPX010 -12243 -9 01 NSE Human ProcartaPlex Serum, plasma, CSF: 25 μL EPX010 -12335 -9 01 NT-3 (NTF3) Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–3,000 pg/mL EHNTF3 NT-4 (NTF4) Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–1,000 pg/mL EHNTF4 NTproBNP Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 11.13 pg/mL 3.64–14,900 pg/mL E PX0 10-12 251-901 NT5E Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 27.67 pg/mL 45.12–184,800 pg/mL EPX010-1230 5-9 01

Oncostatin M (OSM)

Oncostatin M receptor (OSMR)

Osteoactivin (GPNMB) Human Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–12,000 pg/mL EHGPNMB

Osteocalcin

Osteopontin (OPN/SPP1)

Osteoprotegerin (OPG)

p38 MAPK (total) Multispecies Coated ELISA Cell lysate: 10 µL <16 pg/mL 31.2–2,000 pg/mL KHO0061 p38 MAPK (total/

phospho)

Human

Human ProcartaPlex Serum, plasma, CSF: 25 μL 18.149 pg/mL 36–145,900 pg/mL EPX 010 -12337-901

Human

Human Coated ELISA

Human

Human Coated ELISA CCS, plasma, serum: 100 μL 14 pg/mL 14–5,000 pg/mL EHNGF

Rat

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.19 pg/mL 7.32–30,000 pg/mL EPX01A-12117-901 Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 11.5 pg/mL 5.05–20,700 pg/mL EPX01A-32117-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.19 pg/mL 7.32–30,000 pg/mL EPX01A-42117-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.9 pg/mL 3.91–16,000 pg/mL EPX01A-52117-901

Human Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–1,000 pg/mL EHOSM Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.8 pg/mL 9.77–40,000 pg/mL EPX01A-12163 -901

Human

Human Coated ELISA Plasma, Serum: 25 µL 0.08 ng/mL 1.56–75 ng/mL K AQ1381 Human Instant ELISA CCS, plasma, serum: 25 µL 0.2 ng/mL 1. 2–75 ng /mL BMS2020INST Mouse Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–2,000 pg/mL EMSPP1

Human

Human Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–900 pg/mL EHTNFRSF11B Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–1,200 pg/mL EMTNFRSF11B

Human

Multispecies

Instant ELISA CCS, plasma, serum: 10 μL 0.03 ng/mL 0.16 –1 0 ng /mL BMS2038INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 11.9 pg/mL 122.07–500,000 pg/mL EPX01A-12038-901

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 635.70 pg/mL

Coated ELISA Serum, plasma, CCS: 10 μL 0.039 ng/mL 0.39 –25 ng/mL BMS2259

Coated ELISA CCS, plasma, serum: 100 μL 0.5 pg/mL 0.5 –150 ng/mL EHNCAM1

ProcartaPlex Serum, plasma, CSF: 25 μL 31.3 pg/mL 54–221,900 pg/mL E PX010-1235 4-9 01

InstantOne

ELISA

InstantOne

ELISA

InstantOne

ELISA

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 4.98–20,400 pg/mL EPX01A-12202-901

Coated ELISA CCS, plasma, serum: 10 µL 6.5 pg/mL 7.80–500 pg/mL BMS2202

Coated ELISA Tissue lysates, cell lysates: 100 μL 15 pg/mL 15–15,0 00 pg/mL ERNGFCL

Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 15 pg/mL 15–15,000 pg /m L ER NGF

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.3 pg/mL 12.2–50,000 pg/mL EPX01A-12066-901

Coated ELISA CCS, plasma, serum: 20 µL 0.26 ng/mL 0.47–30 ng/mL BMS2066

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.2 pg/mL 7.3–30,000 pg/mL EPX01A-12021-901

Instant ELISA CCS, plasma, serum: 50 µL 2.5 pg/mL 15.6–1,000 pg /mL BMS2021INST

InstantOne

ELISA

Cell lysate: 20 µL 85-86083

Cell lysate: 20 µL 85-86082

Cell lysate: 20 µL 85-86081

CCS, plasma, serum, tissue homogenate, urine: 10 μL

CCS, plasma, serum: 10 μL 312.5 pg/mL 312.5–20,000 pg/mL 88-50330-22

Cell lysate: 20 µL 85-86023

<2 pg/mL 4–1,000 pg/mL KIT036

0.222 μM nitrite;

0.625 μM nitrate

2,610.55 –2,673,20 0 pg/mL

0–100 μM EMSNO

EPX010 -12259 -9 01

Target Species Format Volum e Sensitivity Range Cat. No.

p38 MAPK (total) Multispecies

p38 MAPK [pThr180/ Tyr 182]

P450 demethylation Human, Rat Activity Kit Serum, plasma, CCS: 95 µL 1.6 pmol/100 µL 1.6–20 pmol/100 µL EIAP450DMT

p53 (total/phospho) Human

p53 (total) Human

p53 colorimetric in-cell Multispecies Coated ELISA NA NA NA 62216 p53 Human Coated ELISA CCS, serum: 50 µL 0.33 U/mL 0.78–50 U/mL BMS256

p70 S6K [total/phospho] Multispecies

p70 S6K [pThr389] Multispecies

p70 S6K (total) Multispecies

PAI-1

Palladium API Multispecies Detection Kit Serum, plasma, CCS: 100 µL 3.1 n M 3.1– 10 0 nM EIAPDAPIF PAPP-A Human Coated ELISA CCS, plasma, serum: 100 μL 0.6 ng/mL 0.6–500 ng/mL E HPAPPA

PARN (DAN)

PARP (cleaved) colorimetric in-cell

PARP (cleaved) near infrared in-cell

PARP (cleaved) [214/215] Human Coated ELISA Cell lysate: 20 µL <0.062 ng/mL 0.156 –10 n g/mL K HO0 741 P-cadherin (CDH3) Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–18,000 pg/mL EHCDH3

PD-1 (CD279)

PD-L1 (CD274)

PD-L2

PDGF-A A

PDGF-AB Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–2,500 pg/mL EHPDGFAB

PDGF-BB

PDGFR alpha Human Coated ELISA CCS, plasma, serum: 100 μL 400 pg/mL 0.4 –120 ng/mL EHPDGFRA PDGFR beta Human Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25–18,000 pg/mL EHPDGFRB

PE CAM-1 (CD3 1)

Perforin Human

Pepsinogen I (PGI) Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–6,000 pg/mL EHPGI Pepsinogen II (PGC) Human Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40 –10,000 pg/mL EHPGC Periostin Human Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 0.08–60 ng/mL EHPOSTN PF-4 Human Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–15,0 00 pg/mL EHPF4

PGFM Multispecies

Multispecies

Monkey Coated ELISA CCS, plasma, serum: 100 μL 20 pg/mL 20–6,000 pg/mL EPSERPINE1 Mouse Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 80–20,000 pg/mL EMSERPINE1

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.03 pg/mL 2.69–11,000 pg/mL EPX01A-42033-901

Human Coated ELISA CCS, plasma, serum: 100 μL 35 pg/mL 35–20,000 pg/mL EHPARN Mouse Coated ELISA CCS, plasma, serum: 100 μL 35 pg/mL 35 –10,000 pg/mL EMPAR N

Multispecies Coated ELISA NA NA NA 62219

Multispecies Coated ELISA NA NA NA 62224

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 321.47 pg/mL 485–1,990,000 pg/mL EPX010-26057-901

Human

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 3.1 pg/mL 26–107,600 pg/mL EPX010-26054-901 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 48.17 pg/mL 48.83–200,000 pg/mL EPX01A-12215-901 Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 31.9 pg/mL 257–1,052,900 pg/mL EPX010-26055-901 Human Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40–30,000 pg/mL EHPDGFA Rat Coated ELISA CCS, plasma, serum: 100 μL 90 pg/mL 90 –6,000 pg/mL ERPD GFA

Canine Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–1,200 pg/mL ECPDGFB Porcine Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–2,500 pg/mL ESPDGFB

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.01 pg/mL 7.32–30,000 pg/mL EPX01A-42071-901

Human

Human Instant ELISA CCS, plasma, serum: 10 µL 0.06 ng/mL 0.47–30 ng/mL BMS229INST Monkey Instant ELISA CCS, plasma, serum, urine: 10 µL 0.06 U/mL 0.48– 30 U/mL BMS651INST

InstantOne

ELISA

InstantOne

ELISA

InstantOne

ELISA

InstantOne

ELISA

InstantOne

ELISA

InstantOne

ELISA

InstantOne

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.4 pg/mL 26.17–107,200 pg/mL EPX01A-12033-901

Coated ELISA 50 µL (1:50 prediluted) 29.0 pg/mL 78–5,000 pg/mL BMS2033

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 5.04 pg/mL 6.1–25,000 pg/mL EPX01A-12214-901

Coated ELISA Cell lysate: 20 µL 1.14 pg/mL 2.34–150 pg /m L B MS2 214

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1.96 pg/mL 4.88–20,000 pg/mL E PX 01A -12212-9 01

Coated ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.01 pg/mL 7.32–30,000 pg/mL EPX01A-12071-901

Coated ELISA 50 µL (1:10 prediluted) 4.6 pg/mL 31.3–2,000 pg/mL BMS2071

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 162.5 pg/mL

Coated ELISA CCS, plasma, serum, urine: 10 µL 0.06 ng/mL 0.47–30 ng/mL BMS229

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 158.4 pg/mL 73.24–30,000 pg/mL E PX0 10-12 30 6-9 01

Coated ELISA Serum, plasma, CCS: 20 μL 11.8 pg/mL 28.1–1,800 pg/mL BMS2306

Competitive

ELISA

Cell lysate: 10 µL <16 pg/mL 31.2–2,000 pg/mL 85-86021

Cell lysate: 20 µL 85-86022

Cell lysate: 20 µL 85 - 86123

Cell lysate: 20 µL 0.33 U/mL 0.78–50 U/mL 85 - 86121

Cell lysate: 20 µL 85-86053

Cell lysate: 20 µL 85-86052

Cell lysate: 10 µL 85-86051

CCS, cell lysate, plasma, serum: 50 µL

Serum, plasma, CCS: 50 μL 20.8 pg/mL 50–3,200 pg/mL EIAPGFM

0.56 pg/mL 4.69–300 pg/mL B MS2212

488.28– 2,000,000 pg/mL

EPX01A-1022 9-9 01

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

PGRPS (PGLYRP1) Human Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–1,500 pg/mL EH P G LY RP1 Pl G F-1 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.23 pg/mL 1.22–5,000 pg/mL EPX01A-12155-901 PKA (protein kinase A) Multispecies Activity Kit Serum, plasma, CCS: 40 µL 0.366 U/mL 5–25 U/mL EIAPKA PLGF (PGF) Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–1,000 pg/mL EHPGF PLGF-2 (PGF) Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–400 pg/mL EMPGF PMN-elastase Human Coated ELISA CCS, CSF, lavage ﬂuid, plasma: 10 µL 1.98 ng/ mL 0 .16 –10 ng/m L BMS269 PPBP (NAP-2) Human Coated ELISA CCS, plasma, serum: 100 μL 8.5 pg/mL 8.5–1,000 pg/mL EHPPBP PPBP (TCK-1) Mouse Coated ELISA CCS, plasma, serum: 100 μL 14 pg/mL 14–8,000 pg/mL EMPPBP PPBP (TCK-1) Rat Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 80 –50,000 pg/mL ERPPBP Pregnanediol-3-

glucuronide (PDG) proBNP (NPPB) Human Coated ELISA CCS, plasma, serum: 100 μL 0.14 n g / m L 0 .14 –10 0 ng /mL EHPRONPPB Procalcitonin (PCT) Human Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30 –20,000 pg/mL EHPCT

Progesterone Multispecies

pro-MMP-9 Mouse Coated ELISA CCS, plasma, serum: 100 μL 50 pg/mL 50–18,000 pg/mL EMMMP9 Prolactin (PRL) Mouse Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30 –20,000 pg/mL EMPRL Prolactin Human Coated ELISA Serum, plasma, CCS: 50 μL 11.7 pg/mL 15.6–1,000 pg /mL EHIAPRL Prolactin receptor Rat Coated ELISA CCS, plasma, serum: 100 μL 400 pg/mL 0.4 –50 ng/mL ERPRLR

Prostaglandin E2 Multispecies

PRSS22 (BSSP4) Mouse Coated ELISA CCS, plasma, serum: 100 μL 60 pg/mL 60–4,000 pg/mL EMPRSS22 PRSS27 (marapsin) Human Coated ELISA CCS, plasma, serum: 100 μL 35 pg/mL 35–10,000 pg/mL EHPRSS27 PRSS8 (prostasin) Human Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–8,000 pg/mL EHPRSS8

P-selectin (CD62P)

PSA free (KLK3) Human Coated ELISA CCS, plasma, serum: 100 μL 45 pg/mL 45–12,000 pg/mL EHKLK3F PSA total (KLK3) Human Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–2,000 pg/mL EHKLK3T P S G L-1 Human Coated ELISA 100 µL (1:20 prediluted) 1 U/mL 1.6 –50 U/ mL BMS255 PTX3 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 30.3575 pg/mL 52–213,900 pg/mL EPX010-12281-9 01 PVR Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 38.28 pg/mL 54.32–222,500 pg/mL EPX010-12307- 90 1 PYY Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 19.53–80,000 pg/mL EPX010 -1219 8- 901

(AGER)

RAGE

RANK (TNFRSF11A) Human Coated ELISA CCS, plasma, serum: 100 μL 0.4 ng/mL 0.4–100 ng/mL EHTNFRSF11A

RANKL

RANTES (CCL5)

RARRES2 (TIG2) Human Coated ELISA CCS, plasma, serum: 100 μL 0.5 ng/mL 0.5–50 ng/mL EHRARRES2 RBP4 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 195.31–800,000 pg/mL EPX01A-12205-901 REG3a Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 12.97 pg/mL 2.08–8,500 pg/mL EPX 010 -12249-9 01

Renin 1 (REN1)

(MOK) Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–1,500 pg/mL EHMOK (STK30) Mouse Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–10,0 00 pg/mL EMSTK30

Multispecies

Mouse Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–6,000 pg/mL EMSELP

Human

Monkey Coated ELISA CCS, serum: 10 µL 1. 3 U /m L 0.8–50 U/mL BMS650-4

Rat Coated ELISA CCS, plasma, serum: 100 μL 300 pg/mL 0.3–300 ng/mL ERAGER Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 0.8 pg/mL 2–1,775 pg/mL EPX010 -12290 -9 01

Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.6 pg/mL 7.76–31,800 pg/mL EPX01A-12005-901 Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 3. 2–13,200 pg /m L EPX01A-26037-901 Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 3.41–14,0 00 p g /m L EPX01A-32005-901

Human Coated ELISA

Feline Coated ELISA CCS, plasma, serum: 100 μL 0.7 pg/mL 0.7–200 pg/mL EFCCL 5

Mouse Coated ELISA

Rat Coated ELISA

Human

Mouse

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.1 pg/mL 85.5–350,000 pg/mL EPX01A-36009-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.61–2,500 pg/mL EPX01A-40287-901

Mouse Coated ELISA CCS, plasma, serum: 100 μL 6 pg/mL 6–50,000 pg/mL EMRE N1 Human Coated ELISA Serum, plasma, CCS: 50 μL 32.6 pg/mL 93.8–6,000 pg/mL BMS2271

Competitive

ELISA

Competitive

ELISA

Competitive

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 95.4 pg/mL

Coated ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 0.61–2,500 pg/mL EPX0 1A-10287-901

Instant ELISA

Instant ELISA CCS, plasma, serum: 50 µL 1.2 pg/mL 7.8–500 pg/mL BMS6009INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.35 pg/mL 2.4–10,000 pg/mL EPX01A-26009-901

Serum, plasma, CCS: 50 μL 0.18 n g /mL 0.39–50 ng/mL EIAPDG

Serum, plasma, CCS: 50 μL 47.9 pg/mL 50–3,200 pg/mL EIAP4C21

CCS: 100 µL; saliva, serum, urine, whole blood: 10 µL

Amniotic ﬂuid, CCS, plasma, serum: 10 µL

CCS, Urine: 50 µL; plasma, serum: 1 µL

Serum, plasma: 50 µL; CCS: 100 µL

Amniotic ﬂuid, CCS, plasma, serum: 10 µL

<15.9 pg/mL 39–5,000 pg/mL EHPGE2

1,464.84–6,000,000 pg/mL

0.20 ng/mL 0.63–400 ng/mL BM S219- 4

<2 pg/mL 51.2–2,000 pg/mL EHRNTS

<5 pg/mL 11.7–750 pg/mL KMC10 31

<20 pg/mL 39–2,500 pg/mL KR C10 31

4.2 pg/mL 31.3–2,000 pg/mL BMS287-2INST

EPX 01A-10219- 901

Target Species Format Volum e Sensitivity Range Cat. No.

Retinol binding protein urinary

Resistin (RETN) Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–1,000 pg/mL EMRETN

Resistin (ADSF)

S100A8 /A9 Human

S100 B Human ProcartaPlex Serum, plasma, CSF: 25 μL 1.981 pg/mL 2–7,500 pg/mL EPX010-12 33 9-901 S6 colorimetric in-cell Multispecies Coated ELISA NA NA NA 62207 S6 near infrared in-cell Multispecies Coated ELISA NA NA NA 62 212 s90K (Mac-2BP) Human Coated ELISA 100 µL (1:100 prediluted) 0.92 ng/mL 12.5 – 20 0 ng /mL BMS23 4

SAA

SAP (pentraxin 2) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.7 pg/mL 35.3–144,600 pg/mL EPX01A-12156-901 sAP O -1/ F as Human Coated ELISA CCS, serum: 10 µL 13.2 pg/mL 15.6 –1,0 00 pg/mL BMS245

SCF

SCGF beta Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 21.1 pg/mL 16.79–739,600 pg/mL EPX01A-12157-901 Sclerostin (SOST) Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–10,0 00 pg/mL EHSOST

SDF-1 alpha (CXCL12a)

SDF-1 beta (CXCL12b) Human Coated ELISA CCS, plasma, serum: 100 μL 35 pg/mL 35–8,000 pg/mL EHCXCL12B Serpin A1 (AAT) Human Coated ELISA CCS, plasma, serum: 100 μL 2 ng/mL 2–500 ng/mL EHSERPINA1 Serpin A4 Human Coated ELISA CCS, plasma, serum: 100 μL 0 .12 n g /mL 0.12 – 3 5 n g /mL EHSERPINA4

ShhN (SHH)

Siglec-5 Human Coated ELISA CCS, plasma, serum: 100 μL 8 pg/mL 8–5,000 pg/mL EHSIGLEC5 Siglec-7 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 34.58 pg/mL 15.38–63,000 pg/mL EPX010-12308- 901

Siglec-9 Human

SLAM (SLAMF1) Human Coated ELISA CCS, plasma, serum: 100 μL 60 pg/mL 60–15,0 00 pg/mL EHSLAMF1 SPINT1 (HAI-1) Human Coated ELISA CCS, plasma, serum: 100 μL 0.8 ng/mL 0.8–200 ng/mL EHSPINT1 SPINT2 (HAI-2) Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–800 pg/mL EHSPINT2

ST2 (IL-33R)

STAT3 n ear inf rar ed in-cell

STAT3 [ pY705] Multispecies Coated ELISA Cell lysate: 10 µL <0.9 U/mL 1.57–100 U/mL KHO0481 STAT5A (t ot al) Human Coated ELISA Cell lysate: 10 µL <1 ng/mL 1. 56–100 n g/mL KHO0751 Superoxide dismutase

(SOD) Survivin (BIRC5) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 7.5 pg/mL 108.08–442,700 pg/mL EPX01A-12158-901 Syndecan 1 (SDC1) Human Coated ELISA CCS, plasma, serum: 100 μL 14 pg/mL 14–4,000 pg/mL EHSDC1 Syndecan-1 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 41.4 pg/mL 13.82–56,600 pg/mL EPX010-12233-901 TACI (TNFRSF13B) Human Coated ELISA CCS, plasma, serum: 100 μL 0.3 ng/mL 0.3– 80 ng/mL EHTNFRSF13B Tac til e Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 67.21 pg/mL 71.04–291,000 pg/mL E PX0 10-12 314-9 01 TARC (CCL17) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.311 pg/mL 0.45 –1,850 pg/mL EPX010-12379-901

Tau [pT181] Human

Multispecies

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 5.15–21,100 pg/mL EPX01A-42040-901

Human Coated ELISA CCS, plasma, serum: 100 μL 500 pg/mL 0.5–300 ng/mL EHSAA1 Mouse Coated ELISA Plasma, serum: 1 µL 0.03 μg/mL 0.062–0.5 μg/mL KMA0021 Multispecies Coated ELISA Plasma, serum: 1 µL <0.5 pg/mL 0.062–0.5 μg/mL KAA0021 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 4.9 pg/mL 32–132,600 pg/mL EPX01A-12136-901 Human ProQuantum CCS, serum, plasma: 2–5 µL <240 pg/mL 256–4,000,000 pg/mL A44839

Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–2,000 pg/mL EHKITLG Mouse Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–2,000 pg/mL EMKITL Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.57 pg/mL 1.59– 6,500 pg/mL EPX01A-12137-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.57 pg/mL 1.59– 6,500 pg/mL EPX01A-42137-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.5 pg/mL 6.01–24,600 pg/mL EPX01B-50508-901

Human Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 80–6,000 pg/mL EHCXCL12A Mouse Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–600 pg/mL EMCXCL12 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 20.5 pg/mL 17.09–70,000 pg/mL EPX01A-12138-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 20.5 pg/mL 3 8. 25 –156,70 0 pg/mL EPX01A-42138-901

Human Coated ELISA CCS, plasma, serum: 100 μL

Mouse

Mouse ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 24.22–99,200 pg/mL EPX01A-26047-901 Human Coated ELISA Serum, plasma, CCS: 10 μL 4.6 pg/mL 4.7–300 pg/mL BMS2231

Multispecies Coated ELISA 62 214

Multispecies Activity Kit Serum, plasma, CCS: 10 µL 0.044 U/mL 0.06–4 U/mL EIASODC

Competitive

ELISA

Coated ELISA 50 µL (1:10 prediluted) 3.1 pg/mL 31–2,000 pg/mL BMS2040

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.8 pg/mL 5.74–23,500 pg/mL EPX01A-12040-901

Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–2,500 pg/mL EHS100A8

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 39.014 pg/mL 60.84–249,000 pg/mL EPX010 -12334- 90 1

Coated ELISA Plasma, Serum: 50 µL; CCS: 100 µL 5 pg/mL 5–1,000 pg/mL EMSHH

Coated ELISA Tissue lysates, cell lysates: 100 μL 5 pg/mL 5–1,200 pg/mL EMSHHCL

Coated ELISA CCS, plasma, serum: 100 μL 130 pg/mL 0.12– 3 0 ng /mL EHSIGLEC9

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1.58 pg/mL 1.86–7,600 pg/mL E PX0 10-1230 9-9 01

Coated ELISA CCS, CSF, tissue homogenate: 10 µL <10 pg/mL 15.6 –1,0 00 pg/mL KHO06 31

ProQuantum CCS, serum, plasma: 2–5 µL <0.05 pg/mL 0.064– 5,000 pg/mL A46739

ProcartaPlex Serum, plasma, CSF: 25 μL 0.737 pg/mL 2–8,000 pg/mL EPX0 10-12 34 9-901

Serum, plasma, CCS: 50 μL 2.9 ng/mL 3.9–1,000 ng/mL EIARBPUR

42 pg/mL in assay diluent C, and 8 pg/mL in assay diluent B

8–10,000 pg/mL EHSHH

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

Tau [pT231] Human ProcartaPlex Serum, plasma, CSF: 25 μL 2.09 pg/mL 3 –13,300 pg /m L EPX010 -123 59 -901

Coated ELISA CCS, CSF: 50 µL <12 pg/mL 31.2–2,000 pg/mL KHB0041

Tau (tota l ) Human

Tau [pS 3 96] Human Coated ELISA CCS, CSF: 50 µL <2 pg/mL 15.6–1,000 pg /m L KHB7031

Tau [pS199]

TAU [pT231] Human Coated ELISA

TAU (tot al) Mouse Coated ELISA Cell lysate, brain homogenate: 2 µL <15 pg/mL 31–2,000 pg/mL K MB7 011 TDP-43 Human ProcartaPlex Serum, plasma, CSF: 25 μL 63.7 pg/mL 96–393,200 pg/mL EP X010 -123 56 -901 TECK (CCL25) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.88 pg/mL 8.96 –36,700 pg/mL EPX010 -123 81-901 Tenascin-C Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 119.2 pg/mL 557–2,281,800 pg/mL EPX0 10-12 321-9 01 TFF-3 (trefoil factor 3) Human Coated ELISA CCS, plasma, serum: 100 μL 1.5 ng /mL 1.5–5 0 0 n g /m L EHTFF3 TFPI (tissue factor

pathway inhibitor)

TGF alpha

TGF beta 1

TGF beta 2 Human Coated ELISA 100 µL (1:50 prediluted) 6.6 pg/mL 31.0–1,000 pg/mL BMS254 TGFb RII (TGF-beta

receptor type-2; TGFBR2)

Thiol Multispecies Detection Kit Serum, plasma, CCS: 100 μL 4.62 nM 78–10,000 nM EIARSHF

Thrombopoietin (THPO)

Thrombospondin 1 (TH BS1)

Thyroid Peroxidase (TPO) Human Coated ELISA CCS, plasma, serum: 100 μL 0.3 ng/mL 0.3–100 ng /mL EHTPO Thyroid Stimulating

Hormone (TSH) Thyroglobulin (TG) Human Coated ELISA CCS, plasma, serum: 100 μL 220 pg/mL 0.22–50 ng/mL EHTG

Thyroxine (T4) Multispecies

Tie -1 Human Coated ELISA CCS, plasma, serum: 100 μL 600 pg/mL 0.6 –500 ng/mL EHTIE1

TIE-2 (CD202b) Human

TIM-3

TIMD-4 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 22.6 pg/mL 60.69–248,600 pg/mL EPX010-12332-9 01

TIM P-1

TIMP-2

TIMP-4 Human Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25–7,000 pg/mL EHTIMP4

Human Coated ELISA

Mouse Coated ELISA

Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–1,800 pg/mL EHTFPI

Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–1,000 pg/mL EHTGFA Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 5.1–21,000 pg/mL EPX01A-12139-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 5.1–21,000 pg/mL EPX01A-42139-901 Human Coated ELISA CCS, plasma, serum: 100 μL 25 pg/mL 25–6,000 pg/mL EHTGFBI Mouse Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 3–1,000 ng/mL EMTGFBI

Human

Human/ mouse

Mouse ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.1–4,500 pg/mL EPX01A-20608-901 Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.3 pg/mL 1.72–7,050 pg/mL EPX01A-30249-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.1–4,500 pg/mL EPX01A-40249 -901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.1–4,500 pg/mL EPX01A-50249-901 Porcine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.1–4,500 pg/mL EPX01A-60249-901

Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 1.5–500 pg/mL EHTGFBR2

Human Coated ELISA CCS, plasma, serum: 100 μL 90 pg/mL 90–6,000 pg/mL EHTHPO Mouse Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–20,000 pg/mL EMTHPO Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.7 pg/mL 94.01–385,100 pg/mL EPX01A-12161-9 01

Human Coated ELISA CCS, plasma, serum: 100 μL 6 ng/mL 6–2,000 ng/mL EHTHBS1

Human Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–2,000 pg/mL EHTSH

Human

Mouse ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 5 pg/mL 23–96,200 pg/mL EPX010-26050-901

Mouse Coated ELISA Tissue lysates, cell lysates: 100 μL 3 pg/mL 3–1,000 pg/mL EMTIMP1CL Rat Coated ELISA Tissue lysates, cell lysates: 100 μL 4 pg/mL 4–3,000 pg/mL ERTIMP1CL Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 26.1 pg/mL 36.6–150,00 0 pg/mL EPX01A-12018-901 Human Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 25–7,000 pg/mL EHTIMP2 Mouse Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 0.08–60 ng/mL EMTIMP2

ProQuantum CCS, serum, plasma: 2–5 µL <10 pg/mL 16–250,000 pg/mL A46738

ProcartaPlex Serum, plasma, CSF: 25 μL 6.1 pg/mL 20–80,000 pg/mL E PX0 10-1234 8-9 01

CCS, cell lysate: 50 µL; CSF: 500 µL; brain homogenate: 5 µL

CCS: 50 µL; cell lysate: 5 µL; brain homogenate: 10 µL

CCS, CSF, Tissue Homogenate: 10 µL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.1–4,500 pg/mL E PX0 1A-10249 -901

Coated ELISA 40 µL (pretreated) 8.6 pg/mL 31–2,000 pg/mL BMS249-4

Uncoated

ELISA

Competitive

ELISA

Coated ELISA CCS, plasma, serum: 25 µL 0.03 ng/mL 0 .16 –10 n g /mL BMS2042

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 18.98 pg/mL 31.23–127,900 pg/mL EPX010-12042- 90 1

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 61.04–250,000 pg/mL EPX01A -122 19-9 01

Coated ELISA CCS, plasma, serum: 10 µL 35.3 pg/mL 78.1–5,000 pg/mL BMS2 219

Plasma, serum: 20 µL; CCS: 100 µL 8 pg/mL 8–1,000 pg/mL 88-8350

Serum, plasma, CCS: 10 μL 0.29 ng/mL 0.06–50 ng/mL E IAT4C

<2 pg/mL (5,000 cells)

<7 pg/mL (5,000 cells)

0.7 U/mL 1.56 –10 0 U/mL KHB8051

15.6 –1,00 0 p g/mL K HB7041

15.6 –1,00 0 p g/mL K MB7041

Target Species Format Volum e Sensitivity Range Cat. No.

Bovine Coated ELISA CCS, plasma, serum: 100 μL 0.1 n g / m L 0 .1 – 3 0 n g /mL EBTNF

Canine

Equine

Human

TNF alpha

TNF beta (LT-alpha)

TNF-RI (60 kDa)

TNF-R1B (80 kDa) Human Coated ELISA CCS, plasma, serum, urine: 10 µL 0.1 0 ng/mL 0.16 –10 ng /mL BMS2 11 TNF-RI Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 79.4 pg/mL 159–650,000 pg/mL EPX01A-102 03 -901 TNF-RII Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.1 pg/mL 1.27–5,200 pg/mL EPX01A -10211-901

tPA Human

TRAIL (CD253)

T R AI L-R 1 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 19.56–80,100 pg/mL EPX01A-12010-901 TRAIL-R2 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 1.94 pg/mL 2.4–9,850 pg/mL EPX01A-12011-901 TRAIL-R3 (TNFRSF10C) Human Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–4,000 pg/mL EHTNFRSF10C TRAIL-R4 (TNFRSF10D) Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10–4,000 pg/mL EHTNFRSF10D TRANCE (TNFSF11) Mouse Coated ELISA CCS, plasma, serum: 100 μL 4 pg/mL 4–2,000 pg/mL EM T NFS F 11 Transferrin Human Coated ELISA CCS, plasma, serum: 100 μL 1 ng/mL 1–750 ng /mL EHTF Trappin-2 (PI3) Human Coated ELISA CCS, plasma, serum: 100 μL 5 pg/mL 5–5,000 pg/mL EHPI3

Porcine

Monkey Coated ELISA CCS, serum: 50 µL 6 U/mL 28.1–1,800 U/mL BMS654

Mouse

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 16.64–65,800 pg/mL EPX01A-40223-901

Rat

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 2.8–11,500 pg/mL EPX01A-40202-901 Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–300 pg/mL EMTNFRSF1A

Human

Monkey

Mouse Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–600 pg/mL EMTNFSF 10

Human

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 12.72–52,100 pg/mL EPX01A-42004-901

Coated ELISA CCS, plasma, serum: 100 μL 2 pg/mL 2–700 pg/mL ECTNF

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.3 pg/mL 4.25–17,400 pg/mL EPX01A-50509-901

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL <0.09 pg/mL 0.5–32 pg/mL K HC3 014

Coated ELISA CCS, plasma, serum: 50 µL 5 pg/mL 23–1,500 pg/mL BMS2034

Instant ELISA

HS Coated

ELISA

Uncoated

ELISA

ProcartaPlex

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 8.54–35,000 pg/mL EPX01A-10223 -901

ProQuantum CCS, plasma, serum: 2–5 μL <0.01 pg/mL 0.0128–5,000 pg/mL A35601

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 2.27 pg/mL 6.47–26,500 pg/mL EPX01A-66053-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.39 pg/mL 3.7–15,00 0 p g/mL EPX01A-20607-901

ProcartaPlex

Uncoated

ELISA

Coated ELISA CCS, plasma, serum: 50 µL 3.7 pg/mL 31.3–2,000 pg/mL BMS 607-3

Instant ELISA CCS, plasma, serum: 50 µL 4 pg/mL 31.3–2,000 pg/mL BMS607/2INST

HS Coated

ELISA

ProQuantum CCS, serum, plasma: 2–5 µL <0.3 pg/mL 0.32–5,000 pg/mL A43658

Coated ELISA CCS, plasma, serum: 50 µL <4 pg/mL 15.6–1,00 0 pg /mL KR C 3 011

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.4 pg/mL 4.9–20,000 pg/mL EPX01A-30622-901

Uncoated

ELISA

Coated ELISA CCS, serum: 50 µL 11 pg/mL 39.1–2,500 pg/mL BMS622

Coated ELISA CCS, plasma, serum: 100 µL 4.6 pg/mL 15.6–1,000 pg /m L BMS202

Instant ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.6 pg/mL 6.1–25,000 pg/mL EPX01A-10202- 901

ProQuantum CCS, serum, plasma: 2–5 µL <0.05 pg/mL 0.064–25,000 pg/mL A42898

Coated ELISA CCS, plasma, serum, urine: 10 µL 0.05 ng/mL 0.8–5 ng/mL BMS203

Instant ELISA CCS, plasma, serum: 10 µL 53 pg/mL 0.78–5 ng/mL BMS203INST

Coated ELISA CCS, serum: 10 µL 0.06 U/mL 0.9–6 U/mL BMS653

Instant ELISA CCS, plasma, serum: 10 µL 0.059 U/mL 0.9–6 U/mL BMS653INST

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 5 pg/mL 42.72–175,000 pg/mL EPX01A-10 25 8-901

Coated ELISA CCS, serum: 10 µL 6 pg/mL 15.6 –1,0 00 pg/mL BMS258-2

Coated ELISA CCS, plasma, serum: 50 µL 5 pg/mL 15.6 –1,0 0 0 p g/mL BMS2004

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 12.72–52,100 pg/mL EPX01A-12004-901

CCS, serum: 100 µL <15.6 pg/mL 15.6–1,000 pg /mL ES S 0017

Amniotic ﬂuid, CCS, plasma, serum, synovial ﬂuid, urine: 50 µL

CCS, plasma, serum: 50 µL 0.13 pg/mL 0.31–20 pg/mL BMS223HS

CCS, plasma, serum: 100 µL 4 pg/mL 4–500 pg/mL 88-73 46

Serum, plasma: 25 µL; CCS: 50 µL 0.215 pg/mL 0.45–1,830 pg/mL

CCS, plasma, serum: 100 µL CS C175 3

Serum, plasma: 25 µL; CCS: 50 µL 0.044 pg/mL 0.34–1,390 pg/mL

CCS, plasma, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 8 8-7324

CCS, plasma, serum: 50 µL 0.75 pg/mL 3.13–200 pg/mL BMS607HS

CCS, plasma, serum: 100 µL 16 pg/mL 16–2,000 pg/mL 88 -7340

Amniotic ﬂuid, CCS, plasma, serum, synovial ﬂuid, urine: 100 µL

1.65 pg/mL 7.8–500 pg/mL BMS223INST

EPXS010-10 223901

EPXS010 -20 607901

4.6 pg/mL 15.6 –1,0 00 pg/mL BMS202INST

Targets across platforms (continued)

Target Species Format Volum e Sensitivity Range Cat. No.

TREM-1 (CD354)

TREM-2 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 9.735 pg/mL 10–41,700 pg/mL E PX0 10-1234 3-9 01

Triiodothyronine (T3) Multispecies

Troponin I (troponin I type 3, cardiac; TNNI3)

Troponin T (troponin T, slow skeletal muscle; TN NT1)

TROY ( TNF RS F19) Human Coated ELISA CCS, plasma, serum: 100 μL 50 pg/mL 0.05 –400 ng/mL EHTNFRSF19

TSLP

TWEAK (CD255)

TWEAK R (TNFRSF12A) Mouse Coated ELISA CCS, plasma, serum: 100 μL 95 pg/mL 95–25,000 pg/mL EMTNFRSF12A

TYRO-3 Human

Ubiquitin 1 RPS27A Human Coated ELISA CCS, plasma, serum: 100 μL 30 pg/mL 30–20,000 pg/mL E HRP S2 7A UCHL1 Human ProcartaPlex Serum, plasma, CSF: 25 μL 663.431 pg/mL 742–3,038,100 pg/mL EPX010-1233 8- 901 ULBP-1 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 83.59 pg/mL 142.75– 584,70 0 pg/mL EPX010-12310-9 01 ULBP-3 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 47.49 pg/mL 19.78–81,000 pg/mL EPX0 10-123 12- 901 ULBP-4 Human ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 68.58 pg/mL 82.30–337,100 pg/mL EPX010 -12313 -901 uPa ( PR A P1) Human Coated ELISA CCS, plasma, serum: 100 μL 10 pg/mL 10– 3,200 pg/mL EHPRAP1

uPAR (PL AUR) Human

Uromodulin (UMOD) Human Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40 –10,000 pg/mL EHUMOD VA P-1 Human Coated ELISA 10 µL (1:100 prediluted) 19 pg/mL 31.3–2,000 pg/mL BMS259

VC AM-1 ( CD106)

VEGF

VEGF-A

VEGF-B Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.6 ng/mL 0.6–16 0 ng/mL EMVEGFB

VEGF-C

VEGF-D

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 0.25 ng/mL 0.25–200 ng/mL EMTREM1

Human

Human Coated ELISA CCS, plasma, serum: 100 μL 0.35 ng/mL 0.35 –25 ng/mL EHTNNT1

Human Coated ELISA CCS, plasma, serum: 100 μL 3 pg/mL 10–6,000 pg/mL EHTSLP Mouse Coated ELISA CCS, plasma, serum: 100 μL 1 pg/mL 1–200 pg/mL EMTSLP

Human

Mouse

Human Coated ELISA CCS, plasma, serum: 100 μL 40 pg/mL 40– 3,000 pg/mL EHTNF SF 12

Human

Mouse Coated ELISA CCS, plasma, serum: 100 μL 1.5 pg/mL 1.5–500 pg/mL EMVCAM1

Human

Rat ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.5 pg/mL 24.93–102,100 pg/mL EPX01A-36046-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.6 pg/mL 9.88–40,500 pg/mL EPX01A-40232-901

Human

Mouse Coated ELISA CCS, plasma, serum: 50 µL 20.0 pg/mL 62.5–4,000 pg/mL BMS619-2

Human

Mouse

Rat

NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.73 pg/mL 5.86–24,000 pg/mL EPX01A-40277-901 Canine ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1 pg/mL 5.15–21,100 pg/mL EPX01A-50277-901

Human Coated ELISA CCS, plasma, serum: 20 µL 0.057 ng/mL 0.23–15 ng/mL BM S297/2 Rat Coated ELISA CCS, plasma, serum: 50 µL 27.0 pg/mL 47–3,000 pg/mL BMS626/2 Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.88 pg/mL 12.21–50,000 pg/mL EPX01A-12076-901 NHP ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.88 pg/mL 12.21–50,000 pg/mL EPX01A-42076-901

Coated ELISA CCS, plasma, serum: 100 μL 70 pg/mL 70–50,000 pg/mL EHTREM1

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 139.3 pg/mL 689–2,820,100 pg/mL E PX0 10-12241- 90 1

Competitive

ELISA

Coated ELISA CCS, plasma, serum: 100 μL 100 pg/mL 100–8,000 pg/mL EHTNNI3

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 9.7 pg/mL 10–41,700 pg/mL E PX010-12 257-901

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.5 pg/mL 3.42–14,000 pg/mL EPX01A-1216 4-901

Uncoated

ELISA

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.7 pg/mL 8.71– 35,700 pg /mL EPX01A-26095-901

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 12.36 pg/mL 20.48–83,900 pg/mL EPX01A-12006-901

Instant ELISA CCS, plasma, serum: 50 µL 9.7 pg/mL 15.6–1,000 pg /mL BMS2006INST

Coated ELISA Serum, plasma, CCS: 2 μL 4.6 pg/mL 4–250 pg/mL BMS2287

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 13.1 pg/mL 24–50,000 pg/mL EPX010 -12287-9 01

Coated ELISA CCS, plasma, serum: 100 μL 15 pg/mL 15–4,000 pg/mL EHPL AUR

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 49.7 pg/mL 53–215,500 pg/mL EPX010-12294- 90 1

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 6.7 pg/mL 11.47–47,000 pg/mL EPX01A-10232- 90 1

Coated ELISA 100 µL (1:50 prediluted) 0.6 ng/mL 3 .15–10 0 ng/mL BMS232

Coated ELISA CCS, plasma, serum: 50 µL <5 pg/mL 23.4–1,500 pg/mL KH G 0111

ProQuantum CCS, plasma, serum: 2–5 μL <0.3 pg/mL 0.32–25,000 pg/mL A35602

Coated ELISA Tissue lysates, cell lysates: 100 μL 10 pg/mL 10–6,000 pg/mL EHVEGFACL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 1.73 pg/mL 5.86–24,000 pg/mL E PX01A-10277- 90 1

Coated ELISA CCS, plasma, serum: 50 µL 7.9 pg/mL 15.6 –1,0 00 pg/mL B MS 277-2

Coated ELISA Tissue lysates, cell lysates: 100 μL 2 pg/mL 2–1,000 pg/mL EMVEGFACL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 1.6– 6,600 pg/mL EPX01A -206 19- 901

ProQuantum CCS, plasma, serum: 2–5 µL <1 pg/mL 1.6–25,000 pg/mL A46562

Coated ELISA Plasma, serum: 50 µL; CCS: 100 µL 2 pg/mL 2–200 pg/mL ERVEGFA

Coated ELISA Tissue lysates, cell lysates: 100 μL 2 pg/mL 2–200 pg/mL ERVEGFACL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 0.2 pg/mL 5.54–22,700 pg/mL EPX01A -3 0619 -9 01

Serum, plasma, CCS: 100 μL 37.4 pg/mL 78–5,000 pg/mL E IAT3 C

CCS, plasma, serum: 100 µL 8 pg/mL 8–1,000 pg/mL 8 8 -74 97

CCS, serum: 100 µL 16 pg/mL 16–2,000 pg/mL 88- 749 0

Target Species Format Volum e Sensitivity Range Cat. No.

VE GF- R1 (FLT-1)

VEGF-R2 (KDR)

VE GF- R3 ( FLT-4) Human

VIS TA ( B7-H 5 ) Human ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 7.618 pg/mL 8.64–35,400 pg/mL EPX010-12327- 90 1 Vitronectin (VTN) Human Coated ELISA CCS, plasma, serum: 100 μL 0.8 ng/mL 0.8–200 ng/mL EHVTN vWF (von Willebrand

factor) WI S P-1 Human Coated ELISA CCS, plasma, serum: 100 μL 50 pg/mL 50–15,0 00 pg/mL EHWISP1 XIAP Human Coated ELISA CCS, plasma, serum: 100 μL 250 pg/mL 0.25– 600 ng/mL EHXIAP

ZAG Human

Human

Mouse Coated ELISA

Mouse Coated ELISA CCS, plasma, serum: 100 μL 80 pg/mL 80–20,000 pg/mL EMVEGFR2

Human

Human Coated ELISA CCS, plasma, serum: 100 μL 0.4 ng/mL 0.4–120 ng/mL EHV WF

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 43.3 pg/mL 48.8–200,000 pg/mL EPX01A-1026 8-9 01

Coated ELISA CCS, plasma, serum: 50 µL 0.03 ng/mL 0 .16 –10 n g /mL BMS268-3

Serum, plasma: 50 µL; CCS: 100 µL

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 13.8 pg/mL 48.8–200,000 pg/mL EPX01A-12019-901

Coated ELISA 50 µL (1:25 prediluted) 7 pg/mL 78–5,000 pg/mL B MS 20 19

ProcartaPlex Serum, plasma: 25 µL; CCS: 50 µL 610.35–2,500,000 EPX01A-12064-901

Coated ELISA 50 µL (1:10 prediluted) 0.03 ng/mL 0.6–40 ng/mL BMS2064

ProcartaPlex Serum, plasma: 25 μL; CCS: 50 μL 97.66–400,000 pg/mL EPX01A-12201-901

Coated ELISA CCS, plasma, serum: 10 µL 0.174 ng /mL 0.23 –15.0 ng/mL BMS2201

8 pg/mL 4.14–3,000 pg/mL EMF LT1

References

1. Hornig M et al. (2015) Distinct plasma immune signatures in ME/CFS are present early in the course of illness. Sci Adv 1:e14 0012 1.

2. Horing M et al. (2016) Cytokine network analysis of cerebrospinal ﬂuid in myalgic encephalomyelitis/chronic fatigue syndrome. Mol Psychiatry 21:261–269.

3. DiCarlo J et al. (2014) Cytokine and chemokine patterns across 100 days after hematopoietic stem cell transplantation in children. Biol Blood Marrow Transplant 20:361–369.

4. Siebert JC et al. (2012) The Stanford Data Miner: a novel approach for integrating and exploring heterogeneous immunological data. J Transl Med 10:62.

5. Witte A et al. Medarex— CXCL10 expression and biological activities in inﬂammatory bowel disease. Paper presented at: The Digestive Disease Week Meeting; 2008 May 19-21; San Diego, CA. (from page 35, QG section.)

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