Invitrogen EVOS XL, AMEX1000 User Manual

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™

EVOS

For transmitted light applications

Catalog Number AMEX1000

Publication Number MAN0007989

Revision B.0

XL Core Imaging System

For Research Use Only. Not for use in diagnostic procedures.

Information in this document is subject to change without notice.

Revision

Date

Description

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Revision history: MAN0007987

B.0 16 October 2017 Update branding, legal/regulatory language, reorganize content.

A.0 10 October 2014

1.0 8 April 2013 New user guide.

Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses.

Manufacturer: Life Technologies Corporation | 22025 20th Ave SE St #100 | Bothell, WA 98021

Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is a registered trademark of Kimberly-Clark Corporation.

Correct standard items included table, update legal and regulatory boilerplate, and logos.

Contents

About this guide .............................................................................................................................................. 5

1. Product information ................................................................................................ 6

Product description ......................................................................................................................................... 6

Standard items included ................................................................................................................................. 6

Instrument exterior components and manual controls .............................................................................. 7

Descriptions of manual controls .................................................................................................................... 8

Onscreen controls ............................................................................................................................................ 9

Descriptions of onscreen controls ............................................................................................................... 10

2. Installation ............................................................................................................ 11

Operating environment ................................................................................................................................ 11

Transport the EVOS™ XL Core to the installation site .............................................................................. 11

Connect power supply .................................................................................................................................. 12

Connect wireless mouse and USB flash drive ........................................................................................... 12

Optional: Install attachable mechanical stage ............................................................................................. 13

Install the glass stage plate ........................................................................................................................... 13

Install the EVOS™ XL Core Imaging System in a cell culture hood ........................................................ 14

3. Operation ............................................................................................................... 15

Basic operation ............................................................................................................................................... 15

Adjust instrument settings ........................................................................................................................... 17

Update software ............................................................................................................................................ 18

4. Care and maintenance .......................................................................................... 19

General care .................................................................................................................................................... 19

Objective lens care ......................................................................................................................................... 19

Stage care ........................................................................................................................................................ 19

Decontamination procedures ....................................................................................................................... 20

Appendix A: Troubleshooting...................................................................................... 21

Image issues ................................................................................................................................................... 21

Mechanical issues .......................................................................................................................................... 22

Appendix B: Specifications ......................................................................................... 23

Appendix C: Install the attachable mechanical stage ................................................. 24

Appendix D: Safety ...................................................................................................... 26

Safety conventions used in this document ................................................................................................. 27

Symbols on instruments ............................................................................................................................... 28

Safety labels on instruments ........................................................................................................................ 30

General instrument safety ............................................................................................................................ 31

Chemical safety .............................................................................................................................................. 32

Chemical waste safety................................................................................................................................... 33

Electrical safety .............................................................................................................................................. 34

Physical hazard safety .................................................................................................................................. 35

Biological hazard safety ................................................................................................................................ 35

Safety and Electromagnetic Compatibility (EMC) Standards ................................................................. 36

Documentation and support ....................................................................................... 37

Audience

This user guide is for laboratory staff operating, maintaining, and analyzing data using the EVOS™ XL Core Imaging System.

User attention words

Two user attention words appear in Thermo Fisher Scientific user documentation.

Safety alert words

Four safety alert words appear in Thermo Fisher Scientific user documentation at

Except for IMPORTANT! safety alerts, each safety alert word in a Thermo Fisher

to Thermo Fisher Scientific instruments (see “Safety symbols”, page 29).

SDSs

The Safety Data Sheets (SDSs) for any chemicals supplied by Thermo Fisher

SDSs, see “Safety Data Sheets (SDS)”, page 37.

About this guide

Each word implies a particular level of observation or action as described below.

points in the document where you need to be aware of relevant hazards. Each alert word—IMPORTANT, CAUTION, WARNING, DANGER—implies a particular level of observation or action, as defined below:

Note: Provides information that may be of interest or help but is not critical to the use of the product.

IMPORTANT! Provides information that is necessary for proper instrument operation, accurate installation, or safe use of a chemical.

IMPORTANT! – Provides information that is necessary for proper instrument operation, accurate installation, or safe use of a chemical.

CAUTION! – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

WARNING! – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury.

DANGER! – Indicates an imminently hazardous situation that, if not avoided, will result in death or serious injury. This signal word is to be limited to the most extreme situations.

Scientific document appears with an open triangle figure that contains a hazard symbol. These hazard symbols are identical to the hazard symbols that are affixed

Scientific are available to you free 24 hours a day. For instructions on obtaining

IMPORTANT! For the SDSs of chemicals not distributed by Thermo Fisher Scientific contact the chemical manufacturer.

1. Product information

EVOS™ XL Imaging

The Invitrogen™ EVOS™ XL Core Imaging System (Cat. No. AMEX1000) is a fully

assist cell counting, and image review.

Product use

For Research Use Only. Not for use in diagnostic procedures.

Before setting up your new EVOS™ XL Core Imaging System, unpack the unit and

•

removable

Product description

System

automated, digital, inverted imaging system. The system is designed for a broad range of transmitted light applications including time-lapse imaging, manual-

Standard items included

accessories, and verify all parts are present. Contact your distributor if anything is missing. Damage claims must be filed with the carrier; the warranty does not cover in-transit damage.

Note: If you do not have your distributor information, contact Technical Support (page 37).

EVOS™ XL Core Imaging System, includes fixed stage (see Note below)

• Wireless mouse

• Power adaptor

• USB flash drive (includes User Guide and Quick

Start Guide)

• Condenser shield, removable

• Glass stage plate (packed in bubble wrap)

• Dust cover

Note: The attachable Mechanical stage is an optional feature available

separately from Thermo Fisher Scientific. For ordering information, contact your distributor or Technical Support (page 37).

IMPORTANT! Wiping the computer supplied with the EVOS Imaging System (i.e., erasing the hard drive to remove all programs, files, and the operating system) voids the product warranty. Do not install third party software or update the operating system.

Condenser shield,

™

XL Core

Mechanical stage controls

The attachable mechanical stage is an optional feature. See “Appendix C: Install the

Instrument exterior components and manual controls

Phase turret

Fixed stage with glass stage plate

Freeze button

Save button

Illumination wheel

Objective turret

attachable mechanical stage” (page 24) for installation and removal.

Coarse focus knob

Fine focus knob

Power switch

Power input jack

USB ports

Stage clip

Stage Y-axis knob

Stage X-axis knob (mechanical stage)

Descriptions of manual controls

This glossary is not alphabetized. Manual controls are listed in the order they are

Stage (page 25).

Power input jack

Power switch

USB ports

Illumination wheel

Coarse & Fine focus

Coarse focus tension Objective turret

Phase turret

Freeze & Save Mouse scroll wheel

normally used, with a separate section for the optional Attachable Mechanical

Plug the power adapter into the power input jack.

Set the power switch to “ | ” to turn the instrument on or “O” to turn it off.

Plug the mouse and USB flash drive into the USB ports.

Use the illumination wheel to control illumination intensity.

Use the coarse focus knobs and fine focus knobs to bring the sample into focus.

knobs

Turn the coarse focus tension control ring clockwise (toward the back of the

control ring

instrument) to tighten the tension. Turn the ring counterclockwise to loosen the tension.

Turn the objective turret to change magnifications. The objective turret clicks into place at each position.

Set the phase turret to the position that corresponds with your selected objective for transmitted light observations. The selector will click into place for each of the following positions:

• BF (for brightfield observations)

• 4/10 PH (for phase observations at 4× or 10×)

• 20/40× PH (for phase observations at 20× or 40×)

buttons

(not shown)

Use the Freeze button to capture an image before saving and return to live view after saving. The Save button saves the file with the current settings (Quick Save base file name and selected image format).

Unless the cursor is over a window, the mouse scroll wheel can control illumination intensity. Roll the scroll wheel away from you for more illumination or toward you for less.

Onscreen controls

Note: Roll over the bottom of screen with the mouse to activate the onscreen controls. Onscreen controls are only available when a mouse is installed.

Illumination slider

Freeze/Live toggle

Save image button

Scalebar

Settings button

Onscreen clock

Descriptions of onscreen controls

This glossary is not alphabetized. Onscreen items are listed from left to right.

Illumination slider

The illumination slider controls the illumination

Freeze/Live button

The Freeze/Live button toggles between Freeze and Live settings.

Save button

The Save button saves the current frozen image to USB

and the flash drive is safe to remove.

Settings button

The Settings button displays or hides the Settings window. See “Adjust

Virtual keyboard

The virtual keyboard window

Onscreen clock

The onscreen clock shows date and time and can easily be set.

intensity when the mouse is installed. To turn off illumination, slide all the way to the left.

Click Freeze to capture an image before saving it. Click Live to return to real-time viewing.

flash drive.

• The icon is gray when no USB is plugged in.

• A green USB icon indicates that a flash drive has been

inserted.

• A red USB icon indicates that a save is in progress and the flash drive should not be removed.

• After the file is saved, the USB icon turns back to green

instrument settings” (page 17) for an itemized explanation of the Settings window options.

allows text entry for file names.

Click Accept when you have finished entering the file name.

Click Clear to reset the text field.

Double-click on the time to reveal the settings and use the + or – icons to increase or decrease the values. Click Set to save changes.

•

2. Installation

Operating environment

Place the instrument on a level surface away from vibrations from other pieces of equipment.

• Allow at least 5 cm (2 in) free space at the back of the LCD monitor housing to allow for proper ventilation and prevent overheating of electronic components.

• Set up the EVOS™ XL Core Imaging System away from direct light sources, such as windows. Ambient room lighting can enter the imaging path and affect the image.

• Operating temperature range: 4°C–32°C (40°C–90°F).

• Relative humidity range: 30–90%.

IMPORTANT

to UV sterilization. UV degrades many materials, including plastic. Damage from UV exposure is not covered under the manufacturer’s warranty.

! EVOS

™

XL Core Imaging System should not be subjected

Transport the EVOS™ XL Core to the installation site

1. Remove the glass stage plate and carry it separately.

2. Lift the instrument by grasping it firmly with both hands under the support arm, balancing the weight as shown.

3. To transport EVOS to a different facility, use the original packaging materials if possible. Always make sure that the instrument is properly cushioned and braced to prevent damage.

IMPORTANT! Never allow EVOS subjected to sudden impact or excessive vibration. Handle the instrument with care to prevent damage.

™

XL Core Imaging System

™

XL Core Imaging System to be

Connect power supply

Connect power

1. Plug the mouse receiver into either of the USB

1. Turn the power switch to the “” (OFF) position before connecting the

adapter

power adapter.

2. Connect the power adapter to the power jack on the back of the instrument base and plug the cord into an outlet.

IMPORTANT! Always use the correct power supply. The power adapter specifications appear on the serial number label and in “Appendix B: Specifications” (page 23). Damage due to an incompatible power adapter is not covered by warranty.

Connect wireless mouse and USB flash drive

ports located on the back of the LCD monitor (right side).

2. Follow the mouse manufacturer's instructions to install the battery, and then switch on the mouse.

3. Insert a USB flash drive into the remaining USB port located on the back of the LCD monitor (right side).

Note: If the flash drive has a data lock feature, be sure it is unlocked.

Follow the procedure in “Appendix C: Install the attachable mechanical stage” (page 24) to install the mechanical stage.

Optional

: Install attachable mechanical stage

Install the glass stage plate

Unpack the glass stage plate and insert it into the stage opening (if a mechanical stage is installed, move it all the way back to expose the stage opening).

Install the EVOS™ XL Core Imaging System in a cell culture hood

The small footprint of the EVOS™ XL Core Imaging System, its simple power

to use in a cell culture hood.

Dimensions

The EVOS™ XL Core Imaging System will fit in cell culture hoods that are at least

to turn the instrument at a slight angle to fit.

Installation

XL Core Imaging System ON.

connection, and its easily-viewed display make it quick to install and convenient

18 inches (457 mm) deep. If your cell culture hood is smaller, it may be necessary

ENGLISH METRIC

HEIGHT, TRANSPORT

HEIGHT, DISPLAY

Note: See the illustrations on page 11 for more details about moving the EVOS

™

1. Remove the glass stage plate and carry it separately.

2. Switch the EVOS™ XL Core Imaging System off and disconnect the power cord.

3. Tilt the LCD screen back until it is nearly parallel with the tabletop.

DEPTH

WIDTH

XL Core Imaging System.

16.0 in 40.6 cm

12.5 in 31.8 cm

13.5 in 34.3 cm

21.0 in 55.3 cm

4. Lift the instrument by grasping it firmly with both hands on the support arm

just behind the condenser.

5. Gently place the instrument on a lab cart and transport it to the cell culture hood.

Note: Verify that the hood sash is raised enough for the instrument to slide underneath (approximately 14.5 inches or higher).

6. Lift the instrument as before and move it into the hood.

7. Tilt the LCD monitor upright.

8. Replace the glass stage plate, connect the power cord, and switch the EVOS™

The EVOS™ XL Core Imaging System has both manual and onscreen operation

XL Core Imaging System using only manual controls.

1. Turn on the instrument using the power switch on the right side of the

3. Place slides with coverslips face up on the sample stage.

3. Operation

Basic operation

controls. Manual controls include the illumination wheel, focus knobs, freeze and save buttons, objective turret, and the mechanical stage X-Y axis knobs.

Onscreen controls appear at the bottom of the screen when the mouse rolls over the controls area (page 9

). Using a mouse is optional; you can operate the EVOS™

Note: See “Instrument exterior components and mechanical controls” (page 7) and “Onscreen controls” (page 9) for the location of the instruments controls, as needed.

instrument base.

Note: Startup takes approximately one minute. If the display remains black after startup, adjust the illumination slider or wheel to turn on the illumination.

2. Plug a USB flash drive into one of the USB ports on the right side of the LCD.

For fixed stage models, place the sample vessel directly on the glass stage plate.

For mechanical stage models, secure the sample in a vessel holder with the stage clip over the stage plate.

Note: Go to thermofisher.com/evosxlcore or contact Technical Support (page 37) for a list of vessel holders available for purchase.

4. Set the objective turret to the desired magnification.

5. Turn the phase turret to the position that corresponds to the selected objective and contrast method.

6. Bring the sample into focus using the coarse and fine-focus knobs.

7. Adjust the illumination intensity if necessary, using the illumination wheel or the onscreen illumination slider .

8. Use the manual or the onscreen Freeze/Live toggle to acquire the image.

9. Use the manual or the onscreen Save button to save the image.

10. If you use the onscreen button and Quick Save is not enabled, the virtual keyboard will open to allow you can enter a file name (Quick Save is automatically enabled with the manual Save button).

During the save process, the Save File indicator appears onscreen. Wait until the file is completely saved before operating any manual or onscreen controls or removing the USB drive.

11. Click the onscreen Freeze/Live toggle or the manual Freeze button to return to live viewing.

Note: The onscreen Freeze/Live toggle switches between Freeze and Live settings. Click Freeze to capture an image before saving it. Click Live to return to real-time viewing.

Note: To set up Quick Save and/or specify the file format, see “Adjust instrument settings” (page 17).

The Settings window provides various controls to customize

Image

Image Resolution/Speed buttons

Contrast &

Color Balance Image Save Format

Quick Save

enter the base file name in the

virtual keyboard.

Software update

See “Update software” (page 18) for update instructions.

Adjust instrument settings

display performance and image file names and formats.

The Settings button toggles to display or hide the Settings window.

Note: If the power is switched off before saving the settings, the system reverts to the most recently saved settings.

Resolution/Speed

Saturation

provide two optimized settings:

• 3MP/Slow produces sharper onscreen images

• 1MP/Fast is helpful for quick scanning

Use the Contrast and Saturation sliders to customize these display attributes. The changes appears immediately, but they are not saved unless you click Save .

Defaults button resets contrast and saturation levels to factory settings.

Click Warm and Cool buttons to adjust the color balance. Changes appear immediately, but they are not saved unless you click Save .

Select a file format (.tif, .jpg or .bmp) for saving your image using the Image Save Format buttons .

The Quick Save option allows for a custom base file name; each file name also includes a three-digit sequence number.

When Quick Save is enabled, a single click of the onscreen Save button saves the file under the custom base file name. The manual Save button (page 7) uses the same base file name.

1. Click Edit under the Quick Save option and

Note: To prevent the system from overwriting files, Quick Save uses the next available sequence number if the USB installed contains any

2. To reset the sequence to zero, click Reset .

3. Select Enabled to enable or disable the Quick Save option.

The Update button is inactive unless a USB drive with an update is installed.

files with the same base file name.

Update software

Periodically, Thermo Fisher Scientific adds functionality and other improvements

your distributor information, contact Technical Support (page 37).

Download software

Software updates are available from the EVOS™ XL Core Imaging System product

your local EVOS™ distributor or by contacting Technical Support (page 37).

Install software

1. Plug the USB flash drive into either one of the USB

4. When update is completed, reboot the system by powering it off and on.

5. Click About to confirm you are running the current software version.

to the EVOS™ user interface. We recommend keeping your EVOS™ XL Core Imaging System up to date with the latest software. If you have any questions about software updates, contact your local EVOS

™

distributor. If you do not have

update

page at thermofisher.com/evosxlcore.

Download the update directly to the top level of a USB flash drive with at least 30 MB available. Do not open or rename the file on your computer; EVOS

™

Core Imaging System will verify and install it during the update process.

Download the current user guide for the EVOS

™

XL Core Imaging System from our website. The updated user guide covers the new software functionality when features are added.

Alternatively, you can get the latest software and documentation updates from

ports located on the LCD back (right side).

2. Click Settings to display the Settings window.

3. Click Update and follow the onscreen instructions.

IMPORTANT! Do not power off, unplug the USB flash drive, or add/remove any devices during the update.

• When cleaning optical elements, use only optical-grade materials to avoid

Clean each objective periodically or when necessary with an optical-grade swab

Clean the stage surface as needed with paper towels or Kimwipes™ laboratory

4. Care and maintenance

General care

scratching soft lens coatings.

• Use the appropriate cleaning solutions for each component, as indicated in the

“Decontamination procedures” (page 20).

• If liquid spills on the instrument, turn off the power immediately and wipe

dry.

• Do not exchange objectives between instruments unless you know that the

components have been approved and recommended by Thermo Fisher Scientific.

• After using, cover the instrument with the supplied dust cover.

IMPORTANT! Never disassemble or service the instrument yourself. Unauthorized repairs may damage the instrument or alter its functionality, which may void your warranty. Contact your local EVOS contact Technical Support (page 37) to arrange for service.

™

distributor or

Objective lens care

and a pre-moistened lens wipe (or lens paper moistened with lens cleaning solution). To avoid scratching the soft lens coatings, use only optical-grade cleaning materials and do not rub the lens.

Note: To protect all optical components of the instrument, use the dust cover when the instrument is not in use.

Stage care

wipes dampened with 70% ethanol. You can remove the mechanical stage (if installed) for cleaning. See “Appendix B: Install the attachable mechanical stage”

24) for removal and installation instructions.

(page

Decontamination procedures

To decontaminate the EVOS™ XL Core Imaging System, follow these procedures:

1. Turn power OFF.

2. Clean the LCD display.

a. Use a soft, dry, lint-free cloth to wipe off any dust from the screen.

b. Clean the LCD display with a non-alcohol based cleaner made for flat-

panel displays.

IMPORTANT! Do not spray cleaning fluid directly onto the screen, as it may drip into the display or optics.

3. Lightly wipe the working surfaces (stage top, focus knobs, objective turret,

housing) with paper towels or Kimwipes 70% ethanol or 4,000 ppm hydrogen peroxide (H

4. If it is necessary to decontaminate the condenser, do not apply solution

directly to the condenser assembly. Instead, select the desired phase ring, and then cover the condenser with clear plastic wrap and wipe the wrap with decontamination solution.

™

laboratory wipes dampened with

2O2

IMPORTANT! Do not allow decontamination solution to get into lubricated areas, such as the stage roller bearings, or any points of rotation such as axles for the stage knobs, condenser wheel, etc. Do not soak any surface in decontamination solution. NEVER spray liquid anywhere on the

™

EVOS

XL Core Imaging System. Always wipe surfaces with dampened

paper towels instead.

IMPORTANT! EVOS

™

XL Core Imaging System should not be subjected to UV sterilization. UV degrades many materials, including plastic. Damage from UV exposure is not covered under the manufacturer’s warranty.

Appendix A: Troubleshooting

Image issues

Problem Possible solutions

Image is too dim, even at brightest setting (particularly at higher magnifications)

Specks, dots, or blurs on image

Uneven focus across screen

Difficulty focusing on coverslipped sample on standard slide

LCD screen is black

Image does not respond to changes in focus or stage position

Onscreen Save button does not respond when clicked

• Set the phase turret to the BF position.

• If a condenser slider from a different EVOS

been inserted into the condenser, remove the slider. It is not necessary to use sliders with this instrument.

Follow the instructions under “Objective lens care” (page 19) to clean the objectives.

Position the sample so that it lies flat on the stage; be sure the sample’s thickness is even.

Place the slide so the coverslip is facing up (long working-distance objectives require a thick optical substrate, and image best through 1.0–1.5 mm of glass or plastic).

• If the image is black but the blue LED below the LCD screen is on, adjust the illumination slider or wheel.

• Move the objective turret so that light shines through the objective.

• Verify that the phase turret on the condenser is not stuck between settings.

• Center the sample over the objective.

• Verify the power supply is connected and the power switch is

on (the blue LED below the LCD screen indicates that the power is on).

Click the Live button to return to real-time observation (note that a red USB icon on the Save button indicates there is an unsaved frozen image, which will be lost unless it is saved before clicking Live).

If the USB icon on the Save button appears gray, plug in a USB. If the system does not recognize a USB that is already plugged in, the problem is likely with that particular USB's format, unless the flash drive's data lock is set. Either unlock the flash drive or try using different USB.

™

instrument has

Onscreen controls are absent

Mouse does not work See “Mechanical issues” (page 22).

• Follow instructions under “Connect wireless mouse and USB flash drive” (page 12) to install the wireless USB mouse. The onscreen controls are only active when a mouse is installed.

• Roll over the bottom of the screen with the mouse to activate the onscreen controls.

Mechanical issues

Problem Possible Solutions

Entire mechanical stage shifts loosely over fixed stage

Vessel does not sit securely when mechanical stage moves

Mouse does not work

Note: For additional technical support, contact your local EVOS your distributor information, you can look it up at thermofisher.com/evos or contact Technical Support (page 37).

Tighten screws under stage. See “Appendix B: Install the attachable mechanical stage” (page 24).

Use the correct vessel holder for the application (refer to the

™

EVOS

Vessel Holders spec sheet, in the documentation USB

included with the instrument packaging).

• Verify the wireless mouse receiver has not been removed from the USB drive.

• Check the battery and replace as needed.

• Remove any nearby wireless devices that may be interfering

with the mouse's signal.

™

distributor. If you do not have

Appendix B: Specifications

™

Model

EVOS (Cat. No. AMEX1100)

XL Core Imaging System

Optics

Objectives

Objective turret

Illumination

Contrast Methods

Condenser

Stage

Focus Mechanism

LCD display

Camera

Image acquisition

Captured images

Output ports

Power supply

Dimensions

Weight

Infinity-corrected optical system; RMS-threaded objectives with 45 mm parfocal distance

4× / 10× / 20×; Plan LWD Phase, infinity optional 2× (no phase) & 40× Ph available

4-position; manual control

LED (50,000+ hour life); adjustable intensity

Transmitted light (brightfield and phase contrast)

3-position turret for brightfield and phase contrast; 60 mm working distance

Fixed stage (optional mechanical stage available)

Coaxial focus knobs with tension control Coarse focus: 38 mm/rev Fine focus: 0.2 mm/rev, precision 0.002 mm

12.1-inch color, 1024 × 768 pixels; adjustable tilt

½-inch, 2048 × 1536, 3.1 Megapixels, COLOR

Embedded operating system with imaging software for image capture and save via mouse or front-mounted manual buttons

24 bits/pixel, full color TIFF, JPG or BMP 2048 × 1536 pixels

2 USB 2.0 ports

AC Adapter; Input 100–240 V, 47–63 Hz; 0.58 A max; Output 12 VDC/2.0 A 24 W max

Operating height: 53.3 cm (21.0 in) Storage/transport height: 36.8 cm (14.5 in) Depth: 40.6 cm (16.0 in) Width: 31.8 cm (12.5 in)

9.1 kg (20.1 lbs)

Appendix C: Install the attachable mechanical stage

The mechanical stage is an optional accessory. You may purchase it separately for

more information (page 37).

Installation/

Follow this procedure to attach

use with the EVOS™ XL Core Imaging System.

Go to the product page thermofisher.com/evosxlcore or Technical Support for

Removal

the mechanical stage to the fixed stage. To remove the stage, do the steps in reverse order.

1. Remove the glass stage plate (not shown) and set aside.

2. Tilt the LCD all the way back and gently place the instrument on its left side.

3. Slide the mechanical stage into position over the right side of the fixed stage .

4. Align the mechanical stage so that the two screws under the mechanical stage align with two holes under the fixed stage .

5. Tighten the left screw by hand first.

6. Use the hex key to tighten the right screw (behind the focus knob).

7. Fully tighten the left screw with the hex key.

8. Stand the instrument upright.

9. Replace the glass stage plate. See “Install the glass stage plate” (page 13) for details.

Stage clip

Use the stage clip to secure vessel holders or large samples (i.e., a T-75 flask or

2. Gently release the stage clip to free the vessel holder.

Stage Y-axis knob

Stage X-axis knob

multi-well vessel) to the mechanical stage. When properly secured, the sample moves with the stage as you turn the X-axis and Y-axis knobs.

1. Pull the stage clip open and place the vessel holder or sample in the back right corner of the mechanical stage opening. Verify that the edges are straight and the sample is level.

Use the stage Y-axis knob for front-back movements to position the sample within the field of view.

Use the stage X-axis knob for left-right movements to position the sample within the field of view.

This section includes the following topics:

Appendix D: Safety

• Safety conventions used in this document

• Symbols on instruments

• Safety labels on instruments

• General instrument safety

• Chemical safety

• Chemical waste safety

• Electrical safety

• Physical hazard safety

• Biological hazard safety

• Laser safety

• Workstation safety

• Safety and electromagnetic compatibility (EMC) standards

• SDSs

Safety alert words

Four safety alert words appear in Thermo Fisher Scientific user documentation at

Definitions

Except for IMPORTANT! safety alerts, each safety alert word in Thermo Fisher

Thermo Fisher Scientific instruments (see “Safety symbols” on page 29).

Safety conventions used in this document

points in the document where you need to be aware of relevant hazards. Each alert word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular level of observation or action:

IMPORTANT! Provides information that is necessary for proper instrument operation, accurate installation, or safe use of a chemical.

CAUTION! – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

WARNING! – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury.

DANGER! – Indicates an imminently hazardous situation that, if not avoided, will result in death or serious injury. This signal word is to be limited to the most extreme situations.

Scientific document appears with an open triangle figure that contains a hazard symbol. These hazard symbols are identical to the hazard icons that are affixed to

Symbols on instruments

Electrical symbols on instruments

The following table describes the electrical symbols that may be displayed on

Thermo Fisher Scientific instruments.

Symbol Description

Indicates the On position of the main power switch.

Indicates the Off position of the main power switch.

Indicates a standby switch by which the instrument is switched on to the Standby condition. Hazardous voltage may be present if this switch is on standby.

Indicates the On/Off position of a push-push main power switch.

Indicates a terminal that may be connected to the signal ground reference of another instrument. This is not a protected ground terminal.

Indicates a protective grounding terminal that must be connected to earth ground before any other electrical connections are made to the instrument.

Indicates a terminal that can receive or supply alternating current or voltage.

Indicates a terminal that can receive or supply alternating or direct current or voltage.

Safety symbols

The following table describes the safety symbols that may be displayed on

product-support documents.

Environmental

instruments

The following symbol applies to all Thermo Fisher Scientific electrical and

Thermo Fisher Scientific instruments. Each symbol may appear by itself or in combination with text that explains the relevant hazard (see “ instruments” on page 30 DANGERS, WARNINGS, and CAUTIONS that occur in the text of this and other

Symbol Description

Indicates that you should consult the manual for further information and to proceed with appropriate caution.

Indicates the presence of an electrical shock hazard and to proceed with appropriate caution.

Indicates the presence of a hot surface or other high-temperature hazard and to proceed with appropriate caution.

Indicates the presence of a laser inside the instrument and to proceed with appropriate caution.

Indicates the presence of moving parts and to proceed with appropriate caution.

Indicates the presence of a biological hazard and to proceed with appropriate caution.

). These safety symbols may also appear next to

Safety labels on

symbols on

Indicates the presence of an ultraviolet light and to proceed with appropriate caution.

electronic products placed on the European market after August 13, 2005.

Symbol Description

Do not dispose of this product as unsorted municipal waste. Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment (WEEE).

European Union customers:

Call your Customer Service representative for equipment pick-up and recycling. Go to thermofisher.com for a list of customer service offices in the European Union.

Safety labels on instruments

The following CAUTION, WARNING, and DANGER statements may be

symbols described in the preceding section.

displayed on Thermo Fisher Scientific instruments in combination with the safety

Hazard symbol

CAUTION! Hazardous chemicals. Read the Safety Data Sheets (SDSs) before handling.

CAUTION! Hazardous waste. Refer to SDS(s) and local regulations for handling and disposal.

DANGER! High voltage. DANGER! Haute tension.

WARNING! To reduce the chance of

electrical shock, do not remove covers that require tool access. No user-serviceable parts are inside. Refer servicing to Thermo Fisher Scientific qualified service personnel.

DANGER! Class 3B visible and/or invisible laser radiation present when open. Avoid exposure to beam.

CAUTION! Moving parts. Crush/pinch hazard.

English Français

ATTENTION! Produits chimiques dangereux. Lire les fiches techniques de sûreté de matériels avant toute manipulation de produits.

ATTENTION! Déchets dangereux. Lire les fiches techniques de sûreté de matériels et la régulation locale associées à la manipulation et l’élimination des déchets.

AVERTISSEMENT! Pour éviter les risques d’électrocution, ne pas retirer les capots dont l’ouverture nécessite l’utilisation d’outils. L’instrument ne contient aucune pièce réparable par l’utilisateur. Toute intervention doit être effectuée par le personnel de service qualifié venant de chez Thermo Fisher Scientific.

DANGER! Rayonnement visible ou invisible d’un faisceau laser de Classe 3B en cas d’ouverture. Evitez toute exposition au faisceau.

ATTENTION! Pièces en mouvement, risque de pincement et/ou d’écrasement.

Moving and lifting

monitors

side and hold it stationary while someone slides the contents out of the box.

Operating the

Ensure that anyone who operates the instrument has:

Data Sheets (SDSs)” on page 37.

Cleaning or

the instrument

General instrument safety

WARNING! PHYSICAL INJURY HAZARD. Use this product only as specified in this document. Using this instrument in a manner not specified by Thermo Fisher Scientific may result in personal injury or damage to the instrument.

CAUTION! PHYSICAL INJURY HAZARD The instrument is to be

the instrument

stand-alone computers and

Things to consider before lifting the computer and/or the monitor:

• Make sure that you have a secure, comfortable grip on the computer or the

• Make sure that the path from where the object is to where it is being moved is

• Do not lift an object and twist your torso at the same time.

• Keep your spine in a good neutral position while lifting with your legs.

• Participants should coordinate lift and move intentions with each other before

• Instead of lifting the object from the packing box, carefully tilt the box on its

moved and positioned only by the personnel or vendor specified in the applicable site preparation guide. If you decide to lift or move the instrument after it has been installed, do not attempt to lift or move the instrument without the assistance of others, the use of appropriate moving equipment, and proper lifting techniques. Improper lifting can cause painful and permanent back injury. Depending on the weight, moving or lifting an instrument may require two or more persons.

WARNING! Do not attempt to lift or move the computer or the monitor without the assistance of others. Depending on the weight of the computer and/or the monitor, moving them may require two or more people.

monitor when lifting.

clear of obstructions.

lifting and carrying.

instrument

decontaminating

• Received instructions in both general safety practices for laboratories and specific safety practices for the instrument.

• Read and understood all applicable Safety Data Sheets (SDSs). See “Safety

CAUTION! Using cleaning or decontamination methods other than those recommended by the manufacturer may compromise the safety or quality of the instrument.

Chemical safety

Chemical hazard

General safety

To minimize the hazards of chemicals:

warning

WARNING! CHEMICAL HAZARD. Before handling any chemicals, refer to the Safety Data Sheet (SDS) provided by the manufacturer, and observe all relevant precautions.

WARNING! CHEMICAL HAZARD. All chemicals in the instrument, including liquid in the lines, are potentially hazardous. Always determine what chemicals have been used in the instrument before changing reagents or instrument components. Wear appropriate eyewear, protective clothing, and gloves when working on the instrument.

WARNING! CHEMICAL STORAGE HAZARD. Never collect or store waste in a glass container because of the risk of breaking or shattering. Reagent and waste bottles can crack and leak. Each waste bottle should be secured in a low-density polyethylene safety container with the cover fastened and the handles locked in the upright position. Wear appropriate eyewear, clothing, and gloves when handling reagent and waste bottles.

guidelines

• Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials. (See “Safety Data Sheets (SDSs)” on page 37.)

• Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). For additional safety guidelines, consult the SDS.

• Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood). For additional safety guidelines, consult the SDS.

• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer’s cleanup procedures as recommended in the SDS.

• Comply with all local, state/provincial, or national laws and regulations related to chemical storage, handling, and disposal.

Chemical waste hazard

Chemical waste

To minimize the hazards of chemical waste:

environmental and health regulations.

Waste disposal

If potentially hazardous waste is generated when you operate the instrument, you

regulations.

Chemical waste safety

CAUTION! HAZARDOUS WASTE. Refer to Safety Data Sheets (SDSs) and local regulations for handling and disposal.

safety guidelines

• Read and understand the Safety Data Sheets (SDSs) provided by the

• Provide primary and secondary waste containers. (A primary waste container

• Minimize contact with chemicals. Wear appropriate personal protective

• Minimize the inhalation of chemicals. Do not leave chemical containers open.

• Handle chemical wastes in a fume hood.

• After emptying the waste container, seal it with the cap provided.

• Dispose of the contents of the waste tray and waste bottle in accordance with

must:

• Characterize (by analysis, if necessary) the waste generated by the particular

• Ensure the health and safety of all personnel in your laboratory.

• Ensure that the instrument waste is stored, transferred, transported, and

IMPORTANT! Radioactive or biohazardous materials may require special handling, and disposal limitations may apply.

manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste.

holds the immediate waste. A secondary container contains spills or leaks from the primary container. Both containers must be compatible with the waste material and meet federal, state, and local requirements for container storage.)

equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). For additional safety guidelines, consult the SDS.

Use only with adequate ventilation (for example, fume hood). For additional safety guidelines, consult the SDS.

good laboratory practices and local, state/provincial, or national

applications, reagents, and substrates used in your laboratory.

disposed of according to all local, state/provincial, and/or national

Electrical safety

Fuses

Power Overvoltage rating

The EVOS™ XL Core Imaging System has an installation (overvoltage) category of

DANGER! ELECTRICAL SHOCK HAZARD. Severe electrical shock can result from operating the EVOS instrument panels in place. Do not remove instrument panels. High‐voltage contacts are exposed when instrument panels are removed from the instrument.

WARNING! FIRE HAZARD. For continued protection against the risk of fire, replace fuses only with fuses of the type and rating specified for the instrument.

DANGER! ELECTRICAL HAZARD. Grounding circuit continuity is vital for the safe operation of equipment. Never operate equipment with the grounding conductor disconnected.

DANGER! ELECTRICAL HAZARD. Use properly configured and approved line cords for the voltage supply in your facility.

DANGER! ELECTRICAL HAZARD. Plug the system into a properly grounded receptacle with adequate current capacity.

™

XL Core Imaging System without its

II, and is classified as portable equipment.

Moving parts

Physical hazard safety

WARNING! PHYSICAL INJURY HAZARD. Moving parts can crush and cut. Keep hands clear of moving parts while operating the instrument. Disconnect power before servicing the instrument.

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, and blood of humans and other animals have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations. Wear appropriate protective eyewear, clothing, and gloves. Read and follow the guidelines in these publications:

In the U.S.:

• U.S. Department of Health and Human Services guidelines published in

Biosafety in Microbiological and Biomedical Laboratories

(stock no. 017-040-00547-4; www.cdc.gov/OD/ohs/biosfty/bmbl4/bmbl4toc.htm)

• Occupational Safety and Health Standards, Bloodborne Pathogens

(29 CFR§1910.1030; www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html)

• Your company’s/institution’s Biosafety Program protocols for working with/handling potentially infectious materials.

• Additional information about biohazard guidelines is available at:

www.cdc.gov

In the EU:

• Check your local guidelines and legislation on biohazard and biosafety precaution, and the best practices published in the World Health Organisation (WHO) Laboratory Biosafety Manual, third edition

www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_ 2004_11/en/

Safety and Electromagnetic Compatibility (EMC) Standards

This section provides information on:

U.S. and Canadian Safety Standards

The CSA C/US Mark signifies that the product meets applicable U.S. and

European Safety

The CE Mark symbolizes that the product conforms to all applicable European

Australian EMC standards

The C-Tick Mark indicates conformity with Australian and New Zealand

• U.S. and Canadian safety standards

• European safety and EMC standards

• Australian EMC standards

Canadian standards, including those from CSA, CSA America, ANSI, ASME, ASSE, ASTM, NSF and UL.

and EMC Standards

Community provisions for which this marking is required. Operation of the instrument is subject to the conditions described in this manual. The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Thermo Fisher Scientific.

standards for electromagnetic compatibility.

Technical support

For the latest services and support information for all locations, visit

Download software updates and patches

Safety Data Sheets (SDS)

Safety Data Sheets (SDSs) are available at thermofisher.com/sds.

Limited product

Life Technologies Corporation and/or its affiliate(s) warrant their products as set

at www.thermofisher.com/support.

Documentation and support

Obtaining support

www.thermofisher.com.

At the website, you can:

• Access worldwide telephone and fax numbers to contact Technical Support and Sales facilities

• Search through frequently asked questions (FAQs)

• Submit a question directly to Technical Support (thermofisher.com/support)

• Search for user documents, SDSs, vector maps and sequences, application

notes, formulations, handbooks, certificates of analysis, citations, and other product support documents

• Obtain information about customer training

•

IMPORTANT! For the SDSs of chemicals not distributed by Thermo Fisher Scientific contact the chemical manufacturer.

warranty

forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at

and-conditions.html

IMPORTANT! Wiping the EVOS™ XL Core Imaging System computer

(i.e., erasing the hard drive to remove all programs, files, and the operating system) voids the product warranty.

. If you have any questions, please contact Life Technologies

www.thermofisher.com/us/en/home/global/terms-

thermofisher.com/support | thermofisher.com/askaquestion

thermofisher.com

16 October 2017

Invitrogen EVOS XL, AMEX1000 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual