Invitrogen EVOS XL, AMEX1000 User Manual
™
EVOS
For transmitted light applications
Catalog Number AMEX1000
Publication Number MAN0007989
Revision B.0
XL Core Imaging System
For Research Use Only. Not for use in diagnostic procedures.
Information in this document is subject to change without notice.
Revision
Date
Description
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM
THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: MAN0007987
B.0 16 October 2017 Update branding, legal/regulatory language, reorganize content.
A.0 10 October 2014
1.0 8 April 2013 New user guide.
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this
product, you accept the terms and conditions of all applicable Limited Use Label Licenses.
Manufacturer: Life Technologies Corporation | 22025 20th Ave SE St #100 | Bothell, WA 98021
Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is
a registered trademark of Kimberly-Clark Corporation.
© 2017 Thermo Fisher Scientific Inc. All rights reserved.
Correct standard items included table, update legal and regulatory boilerplate, and
logos.
Contents
About this guide .............................................................................................................................................. 5
1. Product information ................................................................................................ 6
Product description ......................................................................................................................................... 6
Standard items included ................................................................................................................................. 6
Instrument exterior components and manual controls .............................................................................. 7
Descriptions of manual controls .................................................................................................................... 8
Onscreen controls ............................................................................................................................................ 9
Descriptions of onscreen controls ............................................................................................................... 10
2. Installation ............................................................................................................ 11
Operating environment ................................................................................................................................ 11
Transport the EVOS™ XL Core to the installation site .............................................................................. 11
Connect power supply .................................................................................................................................. 12
Connect wireless mouse and USB flash drive ........................................................................................... 12
Optional: Install attachable mechanical stage ............................................................................................. 13
Install the glass stage plate ........................................................................................................................... 13
Install the EVOS™ XL Core Imaging System in a cell culture hood ........................................................ 14
3. Operation ............................................................................................................... 15
Basic operation ............................................................................................................................................... 15
Adjust instrument settings ........................................................................................................................... 17
Update software ............................................................................................................................................ 18
4. Care and maintenance .......................................................................................... 19
General care .................................................................................................................................................... 19
Objective lens care ......................................................................................................................................... 19
Stage care ........................................................................................................................................................ 19
Decontamination procedures ....................................................................................................................... 20
Appendix A: Troubleshooting...................................................................................... 21
Image issues ................................................................................................................................................... 21
Mechanical issues .......................................................................................................................................... 22
Appendix B: Specifications ......................................................................................... 23
Appendix C: Install the attachable mechanical stage ................................................. 24
3
Appendix D: Safety ...................................................................................................... 26
Safety conventions used in this document ................................................................................................. 27
Symbols on instruments ............................................................................................................................... 28
Safety labels on instruments ........................................................................................................................ 30
General instrument safety ............................................................................................................................ 31
Chemical safety .............................................................................................................................................. 32
Chemical waste safety................................................................................................................................... 33
Electrical safety .............................................................................................................................................. 34
Physical hazard safety .................................................................................................................................. 35
Biological hazard safety ................................................................................................................................ 35
Safety and Electromagnetic Compatibility (EMC) Standards ................................................................. 36
Documentation and support ....................................................................................... 37
4
Audience
This user guide is for laboratory staff operating, maintaining, and analyzing data
using the EVOS™ XL Core Imaging System.
User attention
words
Two user attention words appear in Thermo Fisher Scientific user documentation.
Safety alert words
Four safety alert words appear in Thermo Fisher Scientific user documentation at
Except for IMPORTANT! safety alerts, each safety alert word in a Thermo Fisher
to Thermo Fisher Scientific instruments (see “Safety symbols”, page 29).
SDSs
The Safety Data Sheets (SDSs) for any chemicals supplied by Thermo Fisher
SDSs, see “Safety Data Sheets (SDS)”, page 37.
About this guide
Each word implies a particular level of observation or action as described below.
points in the document where you need to be aware of relevant hazards. Each
alert word—IMPORTANT, CAUTION, WARNING, DANGER—implies a
particular level of observation or action, as defined below:
Note: Provides information that may be of interest or help but is not
critical to the use of the product.
IMPORTANT! Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
IMPORTANT! – Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
CAUTION! – Indicates a potentially hazardous situation that, if not
avoided, may result in minor or moderate injury. It may also be used to
alert against unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.
Scientific document appears with an open triangle figure that contains a hazard
symbol. These hazard symbols are identical to the hazard symbols that are affixed
Scientific are available to you free 24 hours a day. For instructions on obtaining
IMPORTANT! For the SDSs of chemicals not distributed by Thermo
Fisher Scientific contact the chemical manufacturer.
5
1. Product information
EVOS™ XL Imaging
The Invitrogen™ EVOS™ XL Core Imaging System (Cat. No. AMEX1000) is a fully
assist cell counting, and image review.
Product use
For Research Use Only. Not for use in diagnostic procedures.
Before setting up your new EVOS™ XL Core Imaging System, unpack the unit and
•
removable
Product description
System
automated, digital, inverted imaging system. The system is designed for a broad
range of transmitted light applications including time-lapse imaging, manual-
Standard items included
accessories, and verify all parts are present. Contact your distributor if anything is
missing. Damage claims must be filed with the carrier; the warranty does not
cover in-transit damage.
Note: If you do not have your distributor information, contact Technical
Support (page 37).
EVOS™ XL Core Imaging System, includes fixed
stage (see Note below)
• Wireless mouse
• Power adaptor
• USB flash drive (includes User Guide and Quick
Start Guide)
• Condenser shield, removable
• Glass stage plate (packed in bubble wrap)
• Dust cover
Note: The attachable Mechanical stage is an optional feature available
6
separately from Thermo Fisher Scientific. For ordering information, contact
your distributor or Technical Support (page 37).
IMPORTANT! Wiping the computer supplied with the EVOS
Imaging System (i.e., erasing the hard drive to remove all programs, files,
and the operating system) voids the product warranty. Do not install third
party software or update the operating system.
Condenser shield,
™
XL Core
Mechanical stage
controls
The attachable mechanical stage is an optional feature. See “Appendix C: Install the
Instrument exterior components and manual controls
Phase turret
Fixed stage with glass stage plate
Freeze button
Save button
Illumination wheel
Objective turret
attachable mechanical stage” (page 24) for installation and removal.
Coarse focus knob
Fine focus knob
Power switch
Power input jack
USB ports
Stage clip
Stage Y-axis knob
Stage X-axis knob
(mechanical stage)
7
Descriptions of manual controls
This glossary is not alphabetized. Manual controls are listed in the order they are
Stage (page 25).
Power input jack
Power switch
USB ports
Illumination wheel
Coarse & Fine focus
Coarse focus tension
Objective turret
Phase turret
Freeze & Save
Mouse scroll wheel
normally used, with a separate section for the optional Attachable Mechanical
Plug the power adapter into the power input jack.
Set the power switch to “ | ” to turn the instrument on or “O” to turn it off.
Plug the mouse and USB flash drive into the USB ports.
Use the illumination wheel to control illumination intensity.
Use the coarse focus knobs and fine focus knobs to bring the sample into focus.
knobs
Turn the coarse focus tension control ring clockwise (toward the back of the
control ring
instrument) to tighten the tension. Turn the ring counterclockwise to loosen the
tension.
Turn the objective turret to change magnifications. The objective turret clicks into
place at each position.
Set the phase turret to the position that corresponds with your selected objective
for transmitted light observations. The selector will click into place for each of the
following positions:
• BF (for brightfield observations)
• 4/10 PH (for phase observations at 4× or 10×)
• 20/40× PH (for phase observations at 20× or 40×)
buttons
(not shown)
Use the Freeze button to capture an image before saving and return to live view
after saving. The Save button saves the file with the current settings (Quick Save
base file name and selected image format).
Unless the cursor is over a window, the mouse scroll wheel can control
illumination intensity. Roll the scroll wheel away from you for more illumination
or toward you for less.
8
Onscreen controls
Note: Roll over the bottom of screen with the mouse to activate the
onscreen controls. Onscreen controls are only available when a mouse is
installed.
Login button
Illumination slider
Freeze/Live toggle
Save image button
Scalebar
Settings button
Onscreen clock
9
Descriptions of onscreen controls
This glossary is not alphabetized. Onscreen items are listed from left to right.
Illumination slider
The illumination slider controls the illumination
Freeze/Live button
The Freeze/Live button toggles between Freeze and Live settings.
Save button
The Save button saves the current frozen image to USB
and the flash drive is safe to remove.
Settings button
The Settings button displays or hides the Settings window. See “Adjust
Virtual keyboard
The virtual keyboard window
Onscreen clock
The onscreen clock shows date and time and can easily be set.
intensity when the mouse is installed. To turn off
illumination, slide all the way to the left.
Click Freeze to capture an image before saving it. Click Live to
return to real-time viewing.
flash drive.
• The icon is gray when no USB is plugged in.
• A green USB icon indicates that a flash drive has been
inserted.
• A red USB icon indicates that a save is in progress and
the flash drive should not be removed.
• After the file is saved, the USB icon turns back to green
instrument settings” (page 17) for an itemized explanation of the Settings
window options.
allows text entry for file names.
Click Accept when you have
finished entering the file name.
Click Clear to reset the text
field.
Double-click on the time to reveal the settings and use the + or –
icons to increase or decrease the values. Click Set to save
changes.
10
•
2. Installation
Operating environment
Place the instrument on a level surface away from vibrations from other pieces
of equipment.
• Allow at least 5 cm (2 in) free space at the back of the LCD monitor housing to
allow for proper ventilation and prevent overheating of electronic components.
• Set up the EVOS™ XL Core Imaging System away from direct light sources,
such as windows. Ambient room lighting can enter the imaging path and
affect the image.
• Operating temperature range: 4°C–32°C (40°C–90°F).
• Relative humidity range: 30–90%.
IMPORTANT
to UV sterilization. UV degrades many materials, including plastic.
Damage from UV exposure is not covered under the manufacturer’s
warranty.
! EVOS
™
XL Core Imaging System should not be subjected
Transport the EVOS™ XL Core to the installation site
1. Remove the glass stage plate and carry it
separately.
2. Lift the instrument by grasping it firmly with
both hands under the support arm, balancing
the weight as shown.
3. To transport EVOS
to a different facility, use the original
packaging materials if possible. Always make
sure that the instrument is properly
cushioned and braced to prevent damage.
IMPORTANT! Never allow EVOS
subjected to sudden impact or excessive vibration. Handle the instrument
with care to prevent damage.
™
XL Core Imaging System
™
XL Core Imaging System to be
11
Connect power supply
Connect power
1. Plug the mouse receiver into either of the USB
1. Turn the power switch to the “” (OFF) position before connecting the
adapter
power adapter.
2. Connect the power adapter to the power jack on the back of the instrument
base and plug the cord into an outlet.
IMPORTANT! Always use the correct power supply. The power adapter
specifications appear on the serial number label and in “Appendix B:
Specifications” (page 23). Damage due to an incompatible power adapter is
not covered by warranty.
Connect wireless mouse and USB flash drive
ports located on the back of the LCD monitor
(right side).
2. Follow the mouse manufacturer's instructions to
install the battery, and then switch on the mouse.
3. Insert a USB flash drive into the remaining USB
port located on the back of the LCD monitor (right
side).
Note: If the flash drive has a data lock feature, be sure it is unlocked.
12
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