Integra 4500, VIAFLO ASSIST Operating Instructions Manual
VIAFLO ASSIST Operating instructions
127950_V07
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the device
complies with:
Zizers, November 4, 2016
Description Model
VIAFLO ASSIST 4500
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU
Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU
Restriction of Hazardous Substances 2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
Radio Equipment Directive 1999/5/EC 13.06.2016 2014/53/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)
1907/2006
Ecodesign - Power supplies 278/2009
Standards for EU
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
EN 61010-1: 2010
Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
EN 61010-2-81: 2015
Electrical equipment for measurement, control and laboratory
use - EMC requirements.
EN 61326-1: 2013
Standards for Canada and USA
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
CAN/CSA-C22.2
No. 61010-1
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
UL 61010-1
Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including
interference that may cause undesired operation.
Part 15 of the
FCC Rules
Contains FCC ID:
PI4410B
Elmar Morscher
CEO
Thomas Neher
Quality Manager
VIAFLO ASSIST – Operating instructions V07
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Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................6
1.3 Safety notes .....................................................................................7
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................8
2.2 Overview of the VIAFLO ASSIST.....................................................8
2.2.1 VIAFLO ASSIST device........................................................8
2.2.2 Keypad..................................................................................8
2.3 Overview of VIAFLO II Electronic Pipette.........................................9
2.3.1 VIAFLO II pipette parts .........................................................9
2.3.2 Display ..................................................................................9
Chapter 3 Installation
3.1 Operating environment...................................................................10
3.2 Relocating VIAFLO ASSIST...........................................................10
3.3 Charging the battery of the VIAFLO II Electronic Pipette ...............10
3.4 Adapting the pipette holder ............................................................10
3.5 Attaching and removing a VIAFLO II Electronic Pipette.................11
3.6 Toolbox settings .............................................................................11
3.6.1 Enabling ASSIST mode ......................................................11
3.6.2 Connection between VIAFLO ASSIST and pipette ............. 11
3.6.3 Setting tip type ....................................................................12
3.6.4 Adjusting the plate alignment..............................................12
3.6.5 Move Speed........................................................................13
Chapter 4 Operation
4.1 Turn on/off the VIAFLO ASSIST device .........................................14
4.2 Turn on/off the VIAFLO II Electronic Pipette ..................................14
4.3 Bluetooth connection ......................................................................14
4.4 Running a program.........................................................................14
4.5 Pipetting in 96 and 384 well plates.................................................15
4.6 Troubleshooting..............................................................................16
VIAFLO ASSIST – Operating instructions V07
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Chapter 5 Programming
5.1 Overview pipetting modes ..............................................................17
5.2 Setting up a program......................................................................18
5.3 Modify existing programs ...............................................................18
5.4 Detailed description of pipetting modes .........................................19
5.4.1 General parameters ............................................................19
5.4.2 Serial Dilution mode............................................................21
5.4.3 Repeat dispense mode .......................................................23
5.4.4 Variable Dispense mode.....................................................25
5.4.5 Multi Aspirate mode ............................................................26
5.4.6 Custom program mode .......................................................27
Chapter 6 Maintenance
6.1 Cleaning .........................................................................................29
6.2 Decontamination ............................................................................29
6.3 Servicing.........................................................................................29
6.4 Equipment disposal ........................................................................29
Chapter 7 Technical Data
7.1 Environmental conditions ...............................................................30
7.2 Specification of the device..............................................................30
7.2.1 Maximum labware height on plate carrier ...........................30
7.3 Intellectual Property........................................................................30
Chapter 8 Accessories
8.1 Accessories for VIAFLO ASSIST ...................................................31
8.2 Accessories for VIAFLO II Electronic Pipettes ...............................31
8.3 Consumables .................................................................................32
Imprint ...................................................................................................35
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1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the VIAFLO ASSIST. This chapter informs about the symbols used in
these operating instructions, the intended use of the VIAFLO ASSIST and the general
safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
W
ARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the co rresponding precautions.
C
AUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
N
OTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
The device is marked with the following symbol:
B
IOHAZARD
The instrument can be potentially biohazardous due to the use of
biozazardous substances by the operator.
VIAFLO ASSIST – Operating instructions V07 Introduction
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1.2 Intended use
This is a general-purpose laboratory instrument for use in research only. Any use of this
instrument in a medical or IVD setting is the sole responsibility of the user.
With a VIAFLO II electronic multichannel pipette attached, VIAFLO ASSIST performs
pipetting operations in microplates automatically.
N
OTE
Only VIAFLO II multichannel pipettes (as of serial number 600 000 and
firmware version 3.00 or higher) can be used with VIAFLO ASSIST. The
VIAFLO II pipette needs its own Bluetooth module, which has to be ordered
separately (part no. 4221).
VIAFLO II Electronic Pipettes are microprocessor controlled and stepper motor driven
pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5–
1250 µl using GripTip pipette tips. Please refer to the VIAFLO II Electronic Pipettes
operating instructions for more detailed informations on www.integra-biosciences.com
.
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1.3 Safety notes
VIAFLO ASSIST complies to the recognized safety regulations and is safe to operate.
VIAFLO ASSIST can only be operated when in intact condition and while observing these
operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the VIAFLO ASSIST must have read and
understood these operating instructions, and particularly, the safety notes, or must have
been instructed by supervisors so that safe operation of the device is guaranteed.
C
AUTION
Do not open or modify the VIAFLO ASSIST in any way . Repairs m ay only be
performed by INTEGRA Biosciences AG or by an authorized after-sales
service member. Parts may be replaced with original INTEGRA Biosciences
parts only.
W
ARNING
Do not use the VIAFLO ASSIST near flammable material or in explosive
areas. Also, do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
N
OTE
Prolonged exposure of the VIAFLO ASSIST to UV-light can cause
discoloration and/or yellowing. However, this will not affect the performance
of the device in any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com
on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.
VIAFLO ASSIST – Operating instructions V07 Description of the device
8 INTEGRA Biosciences AG
2 Description of the device
2.1 Scope of delivery
• VIAFLO ASSIST (VIAFLO II Electronic Pipette to be ordered separately)
• Mains adapter
• Sample pack 10 ml, 25 ml and 100 ml reagent reservoir
C
AUTION
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is dama ged,
instead contact your local dealer.
2.2 Overview of the VIAFLO ASSIST
2.2.1 VIAFLO ASSIST device
2.2.2 Keypad
1 Tower with pipette arm. Arm moves in Z-direction
2 Plate carrier, moves in X-direction
3 Power connection
4 USB port, for firmware updates
5 Keypad
6 Reservoir position
7 Instrument base
8 Pipette holder on pipette arm with lever to shift the
pipette in Y-direction
9 Gripper, lever to release the pipette
10 VIAFLO II Electronic Pipette
11 Up and Down arrow keys move the pipette
along the Z-axis to teach pipetting heights.
12 Left and Right arrow keys move the plate
carrier along the X-axis to adjust the plate carrier
position.
13 Start/Pause key, to start/stop operations.
Green LED blinks: press to start operation/homing
Green LED lights: operation performed
14 Bluetooth control LED (lights blue if connection is
active) and Error LED (flashes red in case of error)
15 On/Off key (lights green if On)
3
9
5
6
8
2
10
1
7
4
11
12
13
14
15
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2.3 Overview of VIAFLO II Electronic Pipette
2.3.1 VIAFLO II pipette parts
2.3.2 Display
The Display shows all pipetting options.
16 Display
17 Back button, to navigate backward
18 Touch wheel, spin to scroll and move the cursor
19 OK button, to make a selection
20 Left and right arrow buttons, for selections
21 Purge button, to empty tips
22 Run key, to start operations
23 Tip ejector
24 Finger hook, facilitates easy operation
25 Volume indicator label, color matches GripTip box
insert.
Battery life indicator
Instruction
Volume
Options
16
17
20
21
22
23
18
19
24
25
Current mode
Active step
OK Serial Dilution,
select program
VIAFLO ASSIST – Operating instructions V07 Installation
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3 Installation
3.1 Operating environment
VIAFLO ASSIST has been designed for use in a laboratory. It shall be operated in a dry
and dust-free location with a temperature of 5–40°C and a maximal (non-condensing)
relative humidity of 80 %, see “7.1
Environmental conditions” on page 30.
3.2 Relocating VIAFLO ASSIST
Before VIAFLO ASSIST can be relocated, clear the Instrument base (7). Switch off the
device and disconnect it from the electricity mains. Lift the VIAFLO ASSIST on both sides
of the Instrument base. Never lift the instrument on the Towe r.
3.3 Charging the battery of the VIAFLO II Electronic Pipette
The battery indicator in the upper right corner of the pipette screen informs about the
battery status. When it turns red, the pipette needs to be charged.
C
AUTION
Use only the approved INTEGRA battery, power supply or charging stand.
Use of an incompatible power transformer can damage the pipette.
The battery can be charged using either the mains adapter or a charging stand, see (“8.2
Accessories for VIAFLO II Electronic Pipettes” on page 31).
3.4 Adapting the pipette holder
The pipette holder can be adapted to the type of the VIAFLO II Electronic Pipette.
Insert the mains adapter connector into the receptacle on the top
back of the pipette. Plug the mains adapter into a wall outlet.
To adapt the holder for an 8- or 16-channel pipette, pull the silver
lever down as shown beside.
Lift the lever up to insert a 12-channel pipette.
VIAFLO ASSIST – Operating instructions V07 Installation
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3.5 Attaching and removing a VIAFLO II Electronic Pipette
Rotate the lower hosing of the VIAFLO II Electronic Pipette by 90 degrees as displayed in
the picture.
3.6 Toolbox settings
3.6.1 Enabling ASSIST mode
The ASSIST mode is hidden from the Main Menu of the VIAFLO II pipettes by default.
3.6.2 Connection between VIAFLO ASSIST and pipette
The first time a specific VIAFLO II pipette is going to be connected to the VIAFLO ASSIST,
both instruments need to be paired. Scroll to the Toolbox and press OK.
To install a pipette, hold it at an angle and insert it into the pipette
holder.
Then lift up until the black gripper snaps in place.
To release the VIAFLO II Electronic Pipette lift the black gripper by
pushing down on one end.
Use the Touch w h e el to select Toolbox from the Main Menu and pre ss
OK.
Select Preferences and then Main Menu. Enable the ASSIST mode by
pressing OK (green ) and press to save your settings.
From the ASSIST option select Bluetooth Pairing.
Switch OFF and ON the VIAFLO ASSIST device, see “4.1
Turn on/off
the VIAFLO ASSIST device” on page 14, and wait approx. 30 sec.
until the message “Pairing successful” is displayed. Press OK.
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