declares on its own responsibility that the devices
DescriptionModels
VIAFLO 966000, 6001
VIAFLO 3846030, 6031
comply with:
EU Directives (DoW: Date of Withdrawal)Before DoWDoWAfter DoW
Low Voltage Equipment2006/95/EC20.04.20162014/35/EU
Electromagnetic Compatibility2004/108/EC20.04.20162014/30/EU
Restriction of Hazardous Substances2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)
Standards for EU
Safety requirements for electrical equipment for measurement,
EN 61010-1: 2010
control and laboratory use - General requirements.
Particular requirements for automatic and semi-automatic
EN 61010-2-81: 2015
laboratory equipment for analysis and other purposes.
Electrical equipment for measurement, control and laboratory use
EN 61326-1: 2013
- EMC requirements.
1907/2006
Standards for Canada and USA
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements
Particular requirements for automatic and semi-automatic laboratory
equipment for analysis and other purposes
a
.
a
.
a
.
Operation is subject to the following two conditions: (1) this device may not
cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
a
NRTL certificate number (TÜV Süd): U8 17 05 42035 007
processing, translation and the form of presentation lie with INTEGRA Biosciences AG.
Neither the complete documentation nor parts thereof may be reproduced in any way, or
stored and processed using electronic media or distributed in any other way without the
written consent of INTEGRA Biosciences AG.
This operating instruction manual has part number 125950, the version is V11. It applies
to software version as of (see Toolbox - Device information):
Control unit (FW)3.10 or higher
Instrument base (HW)3.20 or higher
until a newer revision is released.
Manufacturer
INTEGRA Biosciences AG
CH-7205 Zizers, Switzerland
T +41 81 286 95 30
F +41 81 286 95 33
INTEGRA Biosciences Corp.
Hudson, NH 03051, USA
T +1 603 578 5800
F +1 603 577 5529
Please contact your local INTEGRA Biosciences AG representative.
To find out name and address of your local representative go to
www.integra-biosciences.com
Further information and operating instructions in other languages are available on
www.integra-biosciences.com
VIAFLO 96 and VIAFLO 384 are separate instruments. The abbreviation VIAFLO 96/384
is used if information is relevant to both instruments. These operating instructions contain
all the information required for installation, operation and maintenance of VIAFLO 96/384.
This chapter informs about the symbols used in these operating instructions, the intended
use of VIAFLO 96/384 and the general safety instructions.
1.1Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
ARNING
W
This safety symbol warns against hazards that could result in injury. Italso
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
AUTION
C
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
NOTE
This symbol identifies important notes regardi ng the correct operation of the
device and labor-saving features.
The device is marked with the following symbols:
IOHAZARD
B
The instrument can be potentially biohazardous due to the use of
biozazardous substances by the operator.
UNPLUG
The power cable must be unplugged before the housing of the instrument is
opened by a service technician.
1.2Intended use
This is general purpose laboratory instrument for use in research only. Any use of this
instrument in a medical or IVD setting is the responsibility of the user.
VIAFLO 96/384 is an electronic 96/384-channel hand held pipette designed for aspirating
and dispensing aqueous solutions in the volume range of 0.5 µl to 1250 µl using GripTip
pipette tips. VIAFLO 96/384 is used like a hand held pipette. The movement and
positioning of the 96-/384-channel pipette is supported by a servo assisted steering
mechanism which allows fast, precise and stress free multichannel pipetting. VIAFLO 96
can only be used with 96-channel pipetting heads, while VIAFLO 384 is compatible to both
96- and 384-channel pipetting heads.
If VIAFLO 96/384 is used in a manner not specified by INTEGRA Biosciences, the
protection provided by the VIAFLO 96/384 may be impaired.
1.3Safety notes
VIAFLO 96/384 complies to the recognized safety regulations and is safe to operate.
VIAFLO 96/384 can only be operated when in perfect condition and while observing these
operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the VIAFLO 96/384 must have read and
understood these operating instructions, and particularly, the safety notes, or must have
been instructed by supervisors so that safe operation of the device is guaranteed.
ARNING
W
Do not use the VIAFLO 96/384 near flammable material or in explosive
areas. Also, do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
AUTION
C
Do not immerse the pipetting head in liquid. The fluid can damage intern al
parts. Avoid pipetting of liquids whose vapors could attack the materials PA
(polyamide), POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrilerubber), CR (chloroprene), silicone. Corrosive vapors could also damage
metallic parts inside the device.
Do not open or modify VIAFLO 96/384 in any way . Th e sheet m eta l must not
be removed. Repairs may only be performed by INTEGRA Biosciences AG
or by an authorized after-sales service member.
Parts may be replaced with original INTEGRA Biosciences parts only.
Never insert the mains adapter of any INTEGRA electronic hand pipette into
the power connector of the control unit (5
NOTE
Prolonged exposure of the VIAFLO 96/384 to UV-light can cause
discoloration and/or yellowing of the control unit. However, this will not affect
the performance of the device in any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com
information regarding REACH classified chemicals contained in our products.
).
on a regular basis for up to date
8INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11Description of the device
4
5
8 A
8 B
10
6
1
2
9
3
7
2Description of the device
2.1Scope of delivery
• VIAFLO 96/384 device
• Power cable
• USB cable type A to B
• Operating instructions
AUTION
C
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is damaged,
instead contact your local dealer.
2.2Overview of VIAFLO 96/384
2.2.1VIAFLO 96/384 device
1 Base unit, to move left and right (X axis)
2 Carrying handle of base unit
3 Pipetting unit, to move up and down (Z axis)
4 Tip load button
5 Control unit, see 2.2.2
6 Knob of Side cover, covers the pipetting head
7 Instrument deck
8 Plate holders on position A and B
9 Plate slider, with front (384), zero (96) and back
(384) position to index 96 or 384 well plates (Y axis)
10 Main switch
www.integra-biosciences.com9
VIAFLO 96/384 – Operating instructions V11Description of the device
11
12
15
13
14
19
Current mode
Active step
OK
2.2.2VIAFLO 96/384 control unit
11 Display
12 Back button, to navigate backward
13 Touch wheel, spin to scroll and move the cursor
14 OK button, to make a selection
15 Left and right arrow buttons, for selections
16 PURGE button, to empty tips
17 RUN key, to start operations
18 Tip ejector
19 Finger hook, facilitates easy operation
2.2.3Display
The Display shows all pipetting options.
10INTEGRA Biosciences AG
Instruction
Volumes
Options
Arrow buttons
to set Z height
VIAFLO 96/384 – Operating instructions V11Description of the device
2.2.4Touch wheel
The Touch wheel is fully operational with only one hand. Rotational finger movements
translate into up and down cursor movement on the display. The Touch wheel is fully
functional with the use of latex gloves.
Move finger on the Touch wheel to choose (and
highlight) an option on the display. Press OK (14
make the selection.
When a setting dial is displayed, spin the Touch wheel
to change the value and press OK.
2.2.5Left and right arrow buttons
At times, you will see and on a display screen. These buttons
are used to select options.
Press to select the option indicated with the left arrow (HELP, in
the example beside). Press to select the option indicated with the
right arrow (RUN, in the example).
2.2.6PURGE button
During pipetting, you can interrupt the current pipetting protocol and purge all remaining
liquid currently in the GripTips. To do so, press PURGE (16
).
) to
The pipette will display a prompt.
To proceed, press and release the RUN key (17
of the dispense, the first step in the current program will be
displayed.
2.2.7RUN key
Press and release the RUN key (17
special pipetting operations.
During dispense, you can press and hold the RUN key to perform a two-step blowout, see
Blowout modes” on page 23.
“4.3.3
www.integra-biosciences.com11
) to initiate aspiration, dispense, mix, purge, and
VIAFLO 96/384 has been designed for use in a laboratory. It shall be operated in a dry and
dust-free location with a temperature of 5– 40 °C and a maximal (non-condensing) relative
humidity of 80 %.
3.2Setting up and moving of the instrument
The VIAFLO 96/384 must be set up on a cleaned, dry and horizontal surface.
.
Hold the Carrying handle (2
Instrument deck (7
) to lift VIAFLO 96/384.
) and the
WARNING
The device must not be carried on the control unit because it will be
damaged.
Leave at least 5 cm of free space on the back for air circulation and to allow easy
connection of the mains cable.
ARNING
W
It must always be possible to manually disconnect the plug of the VIAFLO 96/
384 from electricity supply. The corresponding socket shall be within easy
reach of the operator and be clearly labeled as the disconnecting device of
VIAFLO 96/384.
Only use a 3 core mains cable with protective earth to connect VIAFLO 96/384 with the
power source. The socket is located on the reverse side of the instrument deck.
Put the Plate holders (8
) on the Instrument deck (7) on Position A and/or B. They can
both be placed on either side. Move the Plate holder from left to right until the two bolts
click into the two holes.
Alternatively, put the three position stage on the Instrument deck (7
) until the bolts click
into the four holes, see “8.1 Accessories” on page 55).
VIAFLO 96/384 must be secured with the “Park Head” function before
carrying.
Before VIAFLO 96/384 can be relocated, the pipetting and base unit must be fixed. Select
“Park Head” from “Calibration & Service” of the Toolbox menu, see “3.4.5 Calibration &
Services” on page 18. Make sure GripTips have been ejected, clear the Instrument deck
(7
) and press the RUN key (17) to start the park routine. The unit moves to the park
position and is anchored on the base with a bolt. Switch off VIAFLO 96/384 and
disconnect it from the electricity mains.
3.3Getting started
Turn on VIAFLO 96/384 (see "Turn on/off the device" on page 21). To adapt the device to
the appropriate applications, select the following functions of the Toolbox menu in this
order:
• Change head: Select the toolbox menu “Change head” and insert the appropriate
pipetting head, see "
• Load 96/384 GripTips, see "Attaching and removing GripTips" on page 21
• Position settings -Head alignment: Define the center of the wells of a 96-or 384-well
plate, see "
• Position settings -Tip align: VIAFLO 96/384 base unit can be moved left and right
(X direction). Tip align locks onto the wells of a plate and helps to guide the tips into the
microplate wells.
• Z-Height: VIAFLO 96/384 pipetting unit can be moved up and down (Z direction). A
minimum height can be defined in every pipetting mode, e. g. to set optimal tip immersion depth, see “4.3.2
• Preferences: Define system parameters, see "Preferences" on page 16.
The Toolbox provides options to adapt the device to appropriate applications, setting
personal preferences, calibration, computer connectivity and storing owner information.
Toolbox modeDescription
Change HeadAllows to change the pipetting head.
Position SettingsSets the tip alignment, head alignment and Z-height.
PreferencesCustomizes the system parameters.
Calibration & ServiceSets calibration and service history options and parks the head.
CommunicationsEnables communication between your VIAFLO 96/384 and a
PC.
Device InformationView your pipette’s serial number, software version and set a
personal ID.
LanguageSets display language.
Write ProtectProtects programs or menu options from modification.
The Help information describes the modes and some settings, press to select the Help
option.
3.4.1Change head
N
OTE
VIAFLO 96 can only be used with 96-channel pipetting heads, while VIAFLO
384 is compatible with both 96- and 384-channel pipetting heads.
Remove the pipetting head from the case.
Make sure the pipetting head is in extended position (see
image to the left). If necessary, pull apart the Piston plate
(a) on the top as far as possible.
To install the appropriate pipetting head select the Toolbox option “Change Head”.
Loosen the knob and remove the Side cover (6
Remove the existing head, if installed.
Push the appropriate pipetting head into the slide block
and make sure the Piston plate (a) slides onto the
brazen rails.
Mount the Side cover, screw the knob tightly and press
OK to continue.
OTE
N
Pipetting heads with a serial number >50274 feature an ejection lever (b) to
facilitate the removal of the head. Pull up the lever to loosen the pipetting
head, then remove it from the instrument.
Check whether the Pipet factor on the display corresponds to the Pipet factor labeled on
the side of the head itself or indicated in the latest calibration certificate. Press OK if the
factors coincide. If they don't, click Edit.
).
With the factor “Pipet” selected click Edit to change
the Pipet factor accordingly. Using the Touch Wheel,
select the digit to be modified, then press OK and use
the Touch Wheel to select a number. Press OK to
confirm. Once finished, press Save to apply the
correct Pipet factor to the instrument.
Reset sets the factor to 1.0000. Changing the factor “Repeat” is only necessary under
special circumstances. Refer to the calibration document (PROTO_VIAFLO96_384_
calibration), available on request.
3.4.2Storage of pipetting heads
Once a pipetting head is removed from VIAFLO 96/384 it must be stored in the appropriate
casing to protect it from dust and mechanical damage.
AUTION
C
Pipetting heads with 384-channels must always be stored in extended
position.
Position settings contain options that help to find correct pipetting positions. These
settings are globally stored and are valid for all pipetting heads. Perform these alignments
with GripTips attached, see “4.2 Attaching and removing GripTips” on page 21.
Position
settings
Tip AlignOptimally aligns the tips to target the center of the wells
Head Alignment
Z HeightActivate to enable overriding of the defined Z-height.
Description Range
on a 96-or 384-well plate.
•Select Position A or B and press OK to activate the tip
alignment.
• Highlight the Strength option and press OK. Set align-
ment support strength 1 (low) to 3 (high). Press OK.
• Column Detent activates column positions of a plate
on Position A or B, e. g. for serial dilutions. Press OK.
Press to save.
Use a 96 well plate for a 96-channel and a 384-well plate
for a 384-channel pipetting head to define the center of
the wells. This setting is required only once per pipetting
head. Move the unit to Position A or B until the selected
position, e.g. A, is displayed.
• Move the unit down and align the tips to the center of
the wells. Select Set to save this position.
• Repeat at position B.
Keep pushing down the control unit to overcome the Zheight, see “4.3.2
Set Z-Position” on page 23.
/
(On/Off)
1-3
(Off)
(A or B)
-
/
(On/Off)
3.4.4Preferences
Preferences customizes your system parameters. Select an option and press OK to
access. After changing desired settings, press to Save.
Preferences
Deck Brightness
16INTEGRA Biosciences AG
Description Range
Sets brightness of deck illumination.
• Use the Touch wheel to change the brightness:
off, 1 (weak) to 10 (bright). Press OK.
SoundSelect an option and press OK to change the status of
Display
Brightness
Main MenuSelect a function to be hidden from the main menu (Off)
Touch
Wheel
PipettingSelect an option and press OK.
Sets the sensitivity of the control unit to move the
pipetting unit and base unit.
• Use the Touch wheel to set a sensitivity 1 (low) to 9
(high). Press OK.
the beep tone between On and Off:
• Step Complete: at the end of a program step
• Program Complete: at program completion
• Purge Key: when PURGE is pressed.
• Messages: when a message appears.
• Error Message: when an error message appears or
when illegal data entry is attempted.
• Touch Wheel: When spinning the Touch wheel.
• Last dispense: before the last dispense in Repeat
Dispense and Variable Dispense.
Sets brightness of the display. Use the Touch wheel to
change the brightness: 1 (dim) to 10 (bright). Press OK.
and press OK:
• Pipet, Repeat Dispense, Dilute, Pipet/Mix, etc.
• The Automatic mode is by default deactivated. To activate it, change the status to “” (On). See section 5.3.3
for a description of the Automatic mode.
Adjust your Touch wheel spin sensitivity.Low, Medium,
• Purge Key Speed: Choose the desired purge speed.
• Blowin Delay: Choose a timed delay between the
blowout and the blowin at the end of a dispense, if no
two step blowout is performed, see “4.3.3
modes” on page 23.
• Extend Volume (not available for 12.5 µl pipetting
head): For pipetting below the volume range specified:
50 μl pipette: (1.0)–2–50 μl
125 μl pipette: (2.0)–5–125 μl
300 μl pipette: (5.0)–10–300–(310) μl
1250 μl pipette: (25)–50–1250 μl
The volumes in brackets refer to extended volumes,
e.g. extend the minimal pipetting volume of a 125 μl
head from 5 μl to 2 μl.
NOTE
Extending the volume range is not recommended. Full functionality and
specified accuracy/precision cannot be guaranteed.
These options enable you to set calibration features, review service history and move the
pipetting head into the parking position.
Calibration
& Services
CalibrationAllows for re-calibration of VIAFLO 96/384 to restore
Calibration
Reminder
Service History
Park HeadFixes the pipetting and base unit for safe transportation.
After changing desired settings, press to Save.
Description Range
accuracy. The calibration factors for Pipet and Repeat
type are displayed.
To edit the calibration volumes, press .
• Target Volume: This is the volume you are interested
in using for the calibration.
• Actual Volume: This is the measured volume obtained
when dispensing the target volume.
• Current Factor: Displays the factor currently in use.
This factor should be the same as specified on the
head or the latest calibration certificate.
• Reset: Resets the correction factor back to the original factory setting. Press to apply the factory setting.
Sets a calibration reminder based on a number of
pipetting cycles. When the calibration reminder is
displayed, press any key to confirm. However, the
reminder will reappear every time the pipette is turned on
until you change the reminder time or use the reset
option.
• Reminder: Press OK to turn the reminder timer On or
Off.
• Cycles: Use the Touch wheel to set a reminder inter-
val for calibration (time in thousands of cycles). Press
to set the timer to the defined calibration interval.
• Remind in/Total Cycles: Displays the residual amount
of cycles before calibration is required.
• Reset: Resets the timer to the defined calibration
interval. Press to enable.
Press to save.
Displays notes of any service that took place on the
VIAFLO 96/384 listed newest entry first.
• Make sure GripTips are ejected, clear the deck and
press the RUN key to start the park routine. The
pipette moves to park position and can then be turned
off.
-
/
(On/Off)
1k - 240k
cycles
-
-
18INTEGRA Biosciences AG
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