Integra VIAFLO 96, VIAFLO 384 Operating Instructions Manual

VIAFLO 96/384 Operating instructions
125950_V11
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
VIAFLO 96 6000, 6001
VIAFLO 384 6030, 6031
comply with:
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU Restriction of Hazardous Substances 2011/65/EU Waste Electrical and Electronic Equipment 2012/19/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)
Standards for EU
Safety requirements for electrical equipment for measurement,
EN 61010-1: 2010
control and laboratory use - General requirements. Particular requirements for automatic and semi-automatic
EN 61010-2-81: 2015
laboratory equipment for analysis and other purposes. Electrical equipment for measurement, control and laboratory use
EN 61326-1: 2013
- EMC requirements.
1907/2006
Standards for Canada and USA
Safety requirements for electrical equipment for measurement, control and laboratory use - General requirements
Safety requirements for electrical equipment for measurement, control and laboratory use - General requirements
Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes
a
.
a
.
a
.
Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interfer­ence received, including interference that may cause undesired operation.
a
NRTL certificate number (TÜV Süd): U8 17 05 42035 007
Zizers, June 19, 2017
Elmar Morscher CEO
Thomas Neher Quality Manager
CAN/CSA-C22.2
No. 61010-1
UL 61010-1
UL 61010-2-81
Part 15 of the
FCC Rules
Class A
VIAFLO 96/384 – Operating instructions V11
Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................7
1.2 Intended use.....................................................................................7
1.3 Safety notes .....................................................................................8
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................9
2.2 Overview of VIAFLO 96/384.............................................................9
2.2.1 VIAFLO 96/384 device..........................................................9
2.2.2 VIAFLO 96/384 control unit.................................................10
2.2.3 Display ................................................................................10
2.2.4 Touch wheel........................................................................11
2.2.5 Left and right arrow buttons ................................................11
2.2.6 PURGE button ....................................................................11
2.2.7 RUN key..............................................................................11
Chapter 3 Installation
3.1 Operating environment...................................................................12
3.2 Setting up and moving of the instrument........................................12
3.3 Getting started................................................................................13
3.4 Toolbox - adapt your VIAFLO 96/384.............................................14
3.4.1 Change head ......................................................................14
3.4.2 Storage of pipetting heads..................................................15
3.4.3 Position Settings .................................................................16
3.4.4 Preferences.........................................................................16
3.4.5 Calibration & Services.........................................................18
3.4.6 Communications .................................................................19
3.4.7 Device Information..............................................................19
3.4.8 Language............................................................................19
3.4.9 Write protect........................................................................20
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VIAFLO 96/384 – Operating instructions V11
Chapter 4 Operation
4.1 Turn on/off the device.....................................................................21
4.2 Attaching and removing GripTips ...................................................21
4.2.1 Loading tips from a complete box.......................................21
4.2.2 Partial tip loading ................................................................22
4.2.3 Ejecting used GripTips........................................................22
4.3 Start pipetting .................................................................................23
4.3.1 Pipetting..............................................................................23
4.3.2 Set Z-Position .....................................................................23
4.3.3 Blowout modes ...................................................................23
4.3.4 Recommendations for pipetting ..........................................24
4.4 Pipetting options and settings ........................................................25
4.4.1 Edit option...........................................................................25
4.4.2 Volume selection.................................................................25
4.4.3 Speed selection ..................................................................26
4.4.4 Custom................................................................................27
4.4.5 Pace....................................................................................27
4.4.6 Count, Mix Cycle and Rows................................................27
4.4.7 Pipetting between 96 and 384 well plates...........................28
4.4.8 Pipetting with the three position stage................................29
4.5 Troubleshooting/FAQ .....................................................................30
Chapter 5 Pipetting modes
5.1 Overview pipetting modes ..............................................................31
5.2 Detailed description of pipetting modes .........................................32
5.2.1 Pipet mode..........................................................................32
5.2.2 Repeat dispense mode.......................................................33
5.2.3 Sample dilute mode ............................................................34
5.2.4 Pipet/mix mode ...................................................................34
5.2.5 Manual pipet mode .............................................................35
5.2.6 Reverse pipet mode............................................................36
5.2.7 Variable dispense mode .....................................................37
5.2.8 Variable aspirate mode.......................................................38
5.2.9 Sample dilute/mix mode......................................................39
5.2.10 Serial dilution mode ..........................................................40
5.3 Custom step-based programming mode ........................................41
5.3.1 Manual custom program .....................................................42
5.3.2 Modify existing programs....................................................44
5.3.3 Automatic custom program.................................................44
5.4 VIALINK..........................................................................................46
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VIAFLO 96/384 – Operating instructions V11
Chapter 6 Maintenance
6.1 Cleaning and Decontamination ......................................................47
6.1.1 Base unit.............................................................................47
6.1.2 Pipetting heads ...................................................................47
6.2 Prolonged period of non-use..........................................................47
6.3 Leak test.........................................................................................48
6.4 Servicing.........................................................................................49
6.4.1 Blocked pipetting unit..........................................................49
6.4.2 Changing O-rings of tip fittings............................................50
6.5 Calibration ......................................................................................51
6.6 Equipment disposal ........................................................................51
Chapter 7 Technical Data
7.1 Environmental conditions ...............................................................52
7.2 Specification of the device..............................................................52
7.3 Intellectual Property........................................................................53
7.4 Pipetting specifications...................................................................54
Chapter 8 Accessories and consumables
8.1 Accessories....................................................................................55
8.2 Consumables .................................................................................56
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VIAFLO 96/384 – Operating instructions V11
Imprint
© 2017 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction,
processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
This operating instruction manual has part number 125950, the version is V11. It applies to software version as of (see Toolbox - Device information):
Control unit (FW) 3.10 or higher Instrument base (HW) 3.20 or higher
until a newer revision is released.
Manufacturer INTEGRA Biosciences AG
CH-7205 Zizers, Switzerland T +41 81 286 95 30 F +41 81 286 95 33
INTEGRA Biosciences Corp.
Hudson, NH 03051, USA T +1 603 578 5800 F +1 603 577 5529
info@integra-biosciences.com www.integra-biosciences.com
Customer service
Please contact your local INTEGRA Biosciences AG representative. To find out name and address of your local representative go to www.integra-biosciences.com
Further information and operating instructions in other languages are available on www.integra-biosciences.com
6 INTEGRA Biosciences AG
.
or on request info@integra-biosciences.com.
VIAFLO 96/384 – Operating instructions V11 Introduction

1 Introduction

VIAFLO 96 and VIAFLO 384 are separate instruments. The abbreviation VIAFLO 96/384 is used if information is relevant to both instruments. These operating instructions contain all the information required for installation, operation and maintenance of VIAFLO 96/384. This chapter informs about the symbols used in these operating instructions, the intended use of VIAFLO 96/384 and the general safety instructions.

1.1 Symbols used

The operating instructions specifically advise of residual risks with the following symbols:
ARNING
W
This safety symbol warns against hazards that could result in injury. Italso indicates hazards for machinery, materials and the environment. It is essential that you follow the corresponding precautions.
AUTION
C
This symbol cautions against potential material damage or the loss of data in a microprocessor controller. Follow the instructions.
NOTE
This symbol identifies important notes regardi ng the correct operation of the device and labor-saving features.
The device is marked with the following symbols:
IOHAZARD
B
The instrument can be potentially biohazardous due to the use of biozazardous substances by the operator.
UNPLUG
The power cable must be unplugged before the housing of the instrument is opened by a service technician.

1.2 Intended use

This is general purpose laboratory instrument for use in research only. Any use of this instrument in a medical or IVD setting is the responsibility of the user.
VIAFLO 96/384 is an electronic 96/384-channel hand held pipette designed for aspirating and dispensing aqueous solutions in the volume range of 0.5 µl to 1250 µl using GripTip pipette tips. VIAFLO 96/384 is used like a hand held pipette. The movement and positioning of the 96-/384-channel pipette is supported by a servo assisted steering mechanism which allows fast, precise and stress free multichannel pipetting. VIAFLO 96 can only be used with 96-channel pipetting heads, while VIAFLO 384 is compatible to both 96- and 384-channel pipetting heads.
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VIAFLO 96/384 – Operating instructions V11 Introduction
If VIAFLO 96/384 is used in a manner not specified by INTEGRA Biosciences, the protection provided by the VIAFLO 96/384 may be impaired.

1.3 Safety notes

VIAFLO 96/384 complies to the recognized safety regulations and is safe to operate. VIAFLO 96/384 can only be operated when in perfect condition and while observing these operating instructions.
The device may be associated with residual risks if it is used or operated improperly by untrained personnel. Any person operating the VIAFLO 96/384 must have read and understood these operating instructions, and particularly, the safety notes, or must have been instructed by supervisors so that safe operation of the device is guaranteed.
ARNING
W
Do not use the VIAFLO 96/384 near flammable material or in explosive areas. Also, do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data sheet (MSDS) and with all safety guidelines such as the use of protective clothing and safety goggles.
AUTION
C
Do not immerse the pipetting head in liquid. The fluid can damage intern al parts. Avoid pipetting of liquids whose vapors could attack the materials PA (polyamide), POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrile­rubber), CR (chloroprene), silicone. Corrosive vapors could also damage metallic parts inside the device.
Do not open or modify VIAFLO 96/384 in any way . Th e sheet m eta l must not be removed. Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after-sales service member.
Parts may be replaced with original INTEGRA Biosciences parts only. Never insert the mains adapter of any INTEGRA electronic hand pipette into
the power connector of the control unit (5
NOTE
Prolonged exposure of the VIAFLO 96/384 to UV-light can cause discoloration and/or yellowing of the control unit. However, this will not affect the performance of the device in any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com information regarding REACH classified chemicals contained in our products.
).
on a regular basis for up to date
8 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Description of the device
4
5
8 A
8 B
10
6
1
2
9
3
7

2 Description of the device

2.1 Scope of delivery

• VIAFLO 96/384 device
• Power cable
• USB cable type A to B
• Operating instructions
AUTION
C
Verify the scope of delivery when unpacking the device and check for potential transportation damage. Do not operate a device that is damaged, instead contact your local dealer.

2.2 Overview of VIAFLO 96/384

2.2.1 VIAFLO 96/384 device
1 Base unit, to move left and right (X axis) 2 Carrying handle of base unit 3 Pipetting unit, to move up and down (Z axis) 4 Tip load button 5 Control unit, see 2.2.2 6 Knob of Side cover, covers the pipetting head 7 Instrument deck 8 Plate holders on position A and B 9 Plate slider, with front (384), zero (96) and back
(384) position to index 96 or 384 well plates (Y axis)
10 Main switch
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VIAFLO 96/384 – Operating instructions V11 Description of the device
11
12
15
13
14
19
Current mode
Active step
OK
2.2.2 VIAFLO 96/384 control unit
11 Display 12 Back button, to navigate backward 13 Touch wheel, spin to scroll and move the cursor 14 OK button, to make a selection 15 Left and right arrow buttons, for selections 16 PURGE button, to empty tips 17 RUN key, to start operations 18 Tip ejector 19 Finger hook, facilitates easy operation
2.2.3 Display
The Display shows all pipetting options.
10 INTEGRA Biosciences AG
Instruction Volumes
Options
Arrow buttons to set Z height
VIAFLO 96/384 – Operating instructions V11 Description of the device
2.2.4 Touch wheel
The Touch wheel is fully operational with only one hand. Rotational finger movements translate into up and down cursor movement on the display. The Touch wheel is fully functional with the use of latex gloves.
Move finger on the Touch wheel to choose (and highlight) an option on the display. Press OK (14 make the selection.
When a setting dial is displayed, spin the Touch wheel to change the value and press OK.
2.2.5 Left and right arrow buttons
At times, you will see and on a display screen. These buttons are used to select options.
Press to select the option indicated with the left arrow (HELP, in the example beside). Press  to select the option indicated with the right arrow (RUN, in the example).
2.2.6 PURGE button
During pipetting, you can interrupt the current pipetting protocol and purge all remaining liquid currently in the GripTips. To do so, press PURGE (16
).
) to
The pipette will display a prompt. To proceed, press and release the RUN key (17
of the dispense, the first step in the current program will be displayed.
2.2.7 RUN key
Press and release the RUN key (17 special pipetting operations.
During dispense, you can press and hold the RUN key to perform a two-step blowout, see
Blowout modes” on page 23.
“4.3.3
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) to initiate aspiration, dispense, mix, purge, and
). Upon completion
VIAFLO 96/384 – Operating instructions V11 Installation
2
7

3 Installation

3.1 Operating environment

VIAFLO 96/384 has been designed for use in a laboratory. It shall be operated in a dry and dust-free location with a temperature of 5– 40 °C and a maximal (non-condensing) relative humidity of 80 %.

3.2 Setting up and moving of the instrument

The VIAFLO 96/384 must be set up on a cleaned, dry and horizontal surface.
.
Hold the Carrying handle (2 Instrument deck (7
) to lift VIAFLO 96/384.
) and the
WARNING
The device must not be carried on the control unit because it will be damaged.
Leave at least 5 cm of free space on the back for air circulation and to allow easy connection of the mains cable.
ARNING
W
It must always be possible to manually disconnect the plug of the VIAFLO 96/ 384 from electricity supply. The corresponding socket shall be within easy reach of the operator and be clearly labeled as the disconnecting device of VIAFLO 96/384.
Only use a 3 core mains cable with protective earth to connect VIAFLO 96/384 with the power source. The socket is located on the reverse side of the instrument deck.
Put the Plate holders (8
) on the Instrument deck (7) on Position A and/or B. They can both be placed on either side. Move the Plate holder from left to right until the two bolts click into the two holes.
Alternatively, put the three position stage on the Instrument deck (7
) until the bolts click
into the four holes, see “8.1 Accessories” on page 55).
12 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Installation
WARNING
VIAFLO 96/384 must be secured with the “Park Head” function before carrying.
Before VIAFLO 96/384 can be relocated, the pipetting and base unit must be fixed. Select “Park Head” from “Calibration & Service” of the Toolbox menu, see “3.4.5 Calibration & Services” on page 18. Make sure GripTips have been ejected, clear the Instrument deck (7
) and press the RUN key (17) to start the park routine. The unit moves to the park position and is anchored on the base with a bolt. Switch off VIAFLO 96/384 and disconnect it from the electricity mains.

3.3 Getting started

Turn on VIAFLO 96/384 (see "Turn on/off the device" on page 21). To adapt the device to the appropriate applications, select the following functions of the Toolbox menu in this order:
Change head: Select the toolbox menu “Change head” and insert the appropriate
pipetting head, see "
Load 96/384 GripTips, see "Attaching and removing GripTips" on page 21
Position settings - Head alignment: Define the center of the wells of a 96-or 384-well
plate, see "
Position settings - Tip align: VIAFLO 96/384 base unit can be moved left and right
(X direction). Tip align locks onto the wells of a plate and helps to guide the tips into the microplate wells.
Z-Height: VIAFLO 96/384 pipetting unit can be moved up and down (Z direction). A
minimum height can be defined in every pipetting mode, e. g. to set optimal tip immer­sion depth, see “4.3.2
Preferences: Define system parameters, see "Preferences" on page 16.
Position Settings" on page 16.
Change head" on page 14.
Set Z-Position” on page 23.
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VIAFLO 96/384 – Operating instructions V11 Installation

3.4 Toolbox - adapt your VIAFLO 96/384

The Toolbox provides options to adapt the device to appropriate applications, setting personal preferences, calibration, computer connectivity and storing owner information.
Toolbox mode Description
Change Head Allows to change the pipetting head. Position Settings Sets the tip alignment, head alignment and Z-height. Preferences Customizes the system parameters. Calibration & Service Sets calibration and service history options and parks the head. Communications Enables communication between your VIAFLO 96/384 and a
PC.
Device Information View your pipette’s serial number, software version and set a
personal ID. Language Sets display language. Write Protect Protects programs or menu options from modification.
The Help information describes the modes and some settings, press  to select the Help option.
3.4.1 Change head
N
OTE
VIAFLO 96 can only be used with 96-channel pipetting heads, while VIAFLO 384 is compatible with both 96- and 384-channel pipetting heads.
The following pipetting heads are available:
Pipetting head Channels Volume range
12.5 µl 96 0.5–12.5 µl 50 µl 96 2–50 µl
125 µl 96 5–125 µl 300 µl 96 10–300 µl
1250 µl 96 50–1250 µl
12.5 µl 384 0.5–12.5 µl 50 µl 384 2–50 µl
125 µl 384 5–125 µl
14 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Installation
a
a
b
Remove the pipetting head from the case. Make sure the pipetting head is in extended position (see
image to the left). If necessary, pull apart the Piston plate (a) on the top as far as possible.
To install the appropriate pipetting head select the Toolbox option “Change Head”.
Loosen the knob and remove the Side cover (6 Remove the existing head, if installed. Push the appropriate pipetting head into the slide block
and make sure the Piston plate (a) slides onto the brazen rails.
Mount the Side cover, screw the knob tightly and press OK to continue.
OTE
N
Pipetting heads with a serial number >50274 feature an ejection lever (b) to facilitate the removal of the head. Pull up the lever to loosen the pipetting head, then remove it from the instrument.
Check whether the Pipet factor on the display corresponds to the Pipet factor labeled on the side of the head itself or indicated in the latest calibration certificate. Press OK  if the factors coincide. If they don't, click Edit.
).
With the factor “Pipet” selected click Edit to change the Pipet factor accordingly. Using the Touch Wheel, select the digit to be modified, then press OK and use the Touch Wheel to select a number. Press OK to confirm. Once finished, press Save to apply the correct Pipet factor to the instrument.
Reset sets the factor to 1.0000. Changing the factor “Repeat” is only necessary under special circumstances. Refer to the calibration document (PROTO_VIAFLO96_384_ calibration), available on request.
3.4.2 Storage of pipetting heads
Once a pipetting head is removed from VIAFLO 96/384 it must be stored in the appropriate casing to protect it from dust and mechanical damage.
AUTION
C
Pipetting heads with 384-channels must always be stored in extended position.
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VIAFLO 96/384 – Operating instructions V11 Installation
3.4.3 Position Settings
Position settings contain options that help to find correct pipetting positions. These settings are globally stored and are valid for all pipetting heads. Perform these alignments with GripTips attached, see “4.2 Attaching and removing GripTips” on page 21.
Position settings
Tip Align Optimally aligns the tips to target the center of the wells
Head Align­ment
Z Height Activate to enable overriding of the defined Z-height.
Description Range
on a 96-or 384-well plate.
•Select Position A or B and press OK to activate the tip alignment.
• Highlight the Strength option and press OK. Set align- ment support strength 1 (low) to 3 (high). Press OK.
Column Detent activates column positions of a plate on Position A or B, e. g. for serial dilutions. Press OK.
Press to save.
Use a 96 well plate for a 96-channel and a 384-well plate for a 384-channel pipetting head to define the center of the wells. This setting is required only once per pipetting head. Move the unit to Position A or B until the selected position, e.g. A, is displayed.
• Move the unit down and align the tips to the center of the wells. Select Set to save this position.
• Repeat at position B.
Keep pushing down the control unit to overcome the Z­height, see “4.3.2
Set Z-Position” on page 23.
/ (On/Off) 1-3
(Off) (A or B)
-
/ (On/Off)
3.4.4 Preferences
Preferences customizes your system parameters. Select an option and press OK to access. After changing desired settings, press to Save.
Preferences
Deck Bright­ness
16 INTEGRA Biosciences AG
Description Range
Sets brightness of deck illumination.
• Use the Touch wheel to change the brightness: off, 1 (weak) to 10 (bright). Press OK.
Off 1-10
VIAFLO 96/384 – Operating instructions V11 Installation
Handle Sen­sitivity
Sound Select an option and press OK to change the status of
Display Brightness
Main Menu Select a function to be hidden from the main menu (Off)
Touch Wheel
Pipetting Select an option and press OK.
Sets the sensitivity of the control unit to move the pipetting unit and base unit.
• Use the Touch wheel to set a sensitivity 1 (low) to 9 (high). Press OK.
the beep tone between On and Off:
Step Complete: at the end of a program step
Program Complete: at program completion
Purge Key: when PURGE is pressed.
Messages: when a message appears.
Error Message: when an error message appears or when illegal data entry is attempted.
Touch Wheel: When spinning the Touch wheel.
Last dispense: before the last dispense in Repeat Dispense and Variable Dispense.
Sets brightness of the display. Use the Touch wheel to change the brightness: 1 (dim) to 10 (bright). Press OK.
and press OK:
• Pipet, Repeat Dispense, Dilute, Pipet/Mix, etc.
• The Automatic mode is by default deactivated. To acti­vate it, change the status to “” (On). See section 5.3.3 for a description of the Automatic mode.
Adjust your Touch wheel spin sensitivity. Low, Medium,
Purge Key Speed: Choose the desired purge speed.
Blowin Delay: Choose a timed delay between the blowout and the blowin at the end of a dispense, if no two step blowout is performed, see “4.3.3 modes” on page 23.
Extend Volume (not available for 12.5 µl pipetting head): For pipetting below the volume range specified: 50 μl pipette: (1.0)–2–50 μl 125 μl pipette: (2.0)–5–125 μl 300 μl pipette: (5.0)–10–300–(310) μl 1250 μl pipette: (25)–50–1250 μl The volumes in brackets refer to extended volumes, e.g. extend the minimal pipetting volume of a 125 μl head from 5 μl to 2 μl.
NOTE
Extending the volume range is not recommended. Full functionality and specified accuracy/precision cannot be guaranteed.
Blowout
1-9
/ (On/Off)
1-10
/ (On/Off)
High
1-10
None/
0.5-5.0 s
/ (On/Off)
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VIAFLO 96/384 – Operating instructions V11 Installation
3.4.5 Calibration & Services
These options enable you to set calibration features, review service history and move the pipetting head into the parking position.
Calibration & Services
Calibration Allows for re-calibration of VIAFLO 96/384 to restore
Calibration Reminder
Service His­tory
Park Head Fixes the pipetting and base unit for safe transportation.
After changing desired settings, press to Save.
Description Range
accuracy. The calibration factors for Pipet and Repeat type are displayed. To edit the calibration volumes, press .
Target Volume: This is the volume you are interested in using for the calibration.
Actual Volume: This is the measured volume obtained when dispensing the target volume.
Current Factor: Displays the factor currently in use. This factor should be the same as specified on the head or the latest calibration certificate.
Reset: Resets the correction factor back to the origi­nal factory setting. Press to apply the factory setting.
Sets a calibration reminder based on a number of pipetting cycles. When the calibration reminder is displayed, press any key to confirm. However, the reminder will reappear every time the pipette is turned on until you change the reminder time or use the reset option.
Reminder: Press OK to turn the reminder timer On or Off.
Cycles: Use the Touch wheel to set a reminder inter- val for calibration (time in thousands of cycles). Press to set the timer to the defined calibration interval.
Remind in/Total Cycles: Displays the residual amount of cycles before calibration is required.
Reset: Resets the timer to the defined calibration interval. Press to enable.
Press to save. Displays notes of any service that took place on the
VIAFLO 96/384 listed newest entry first.
• Make sure GripTips are ejected, clear the deck and press the RUN key to start the park routine. The pipette moves to park position and can then be turned off.
-
/ (On/Off) 1k - 240k cycles
-
-
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VIAFLO 96/384 – Operating instructions V11 Installation
3.4.6 Communications
VIAFLO 96/384 can be programmed from a PC via USB communication cable (type A to B).
Communi­cations
VIALINK Connect the USB cable betweenVIAFLO 96/384 and a PC. Press OK to
3.4.7 Device Information
Device Information
VIAFLO 96/ 384
In addition, information about your VIAFLO 96/384, such as serial number, the firmware (FW) and hardware (HW) version of the control unit and of the base unit are displayed.
3.4.8 Language
Language Description
Language You can choose the language in which all screens are displayed. Scroll to
Description
begin bi-directional communication. To exit the communications mode turn off VIAFLO 96/384.
Description
Owner: Press to enter the user name for your pipette. Use the Touch Wheel to highlight a character and press OK. You can press to Delete
the last character entered. After entering the desired text, press to Save.
the desired language, press OK and to Save.
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VIAFLO 96/384 – Operating instructions V11 Installation
3.4.9 Write protect
Select this option to protect programs and menu options from inadvertent modification. The pipetting programs can still be used.
Write protect
Keep the password in a safe place. Should you lose your password, contact INTEGRA Biosciences to retrieve your password.
Description Range
Select an option and press OK to switch protection on or off:
Standard Programs
Custom Programs
Calibration
Too lbo x
Password Protection: Protect the access to the write protect menu by selecting “”.
Edit Password, if password protect is switched on. To enter a password use the Touch wheel to highlight a character and press OK. Press to save the pass­word. The password must be entered before you can access the write protect menu.
/ (On/Off)
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VIAFLO 96/384 – Operating instructions V11 Operation

4 Operation

4.1 Turn on/off the device

Turn on:
AUTION
C Remove hands from Control unit (5) at switch on and during homing.
When VIAFLO 96/384 is turned on by the Main switch (10), you are prompted to press the RUN key (17
Press the RUN key again to home the pipetting unit. If filled tips are still on the device, put a basin below the pipetting head. After homing the Main menu is displayed.
Turn off:
To turn off VIAFLO 96/384, press the Main switch (10

4.2 Attaching and removing GripTips

) to perform a vertical and horizontal homing routine.
).
NOTE
VIAFLO 96/384 automatically goes into stand-by after 30 minutes of inactivity. Press OK to continue.
CAUTION
To ensure optimal performance of your VIAFLO 96/384, use only GripTips designated for use with VIAFLO 96/384, see “8 Accessories and consumables” on page 55. To prevent contamination of VIAFLO 96/384 pipetting heads, it is recommended to use filtered GripTips only.
NOTE
When using 12.5 µl or 125 µl GripTips with a 96-channel pipetting head, put the tip box on a Plate holder with slide function and move the Plate slider
) to a 384 position, see “4.4.7 Pipetting between 96 and 384 well plates” on
(9 page 28.
4.2.1 Loading tips from a complete box
Put a tip box either on the left or right Plate holder (8). Hold the Control unit (5) and lower the pipetting head down onto the tip box until the Tip load button (4) flashes.
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VIAFLO 96/384 – Operating instructions V11 Operation
a
b
When prompted push the Tip load button (a) and at the same time push down the Control unit (b).
When the tips are loaded, the Tip load button lights up.
) up until the light of the Tip
4.2.2 Partial tip loading
C
AUTION
If only one tip column should be loaded, reduced loading force is required.
Move the Pipetting unit (3 load button turns off.
Put a tip box either on the left or right Plate holder (8
). Hold the Control unit (5) and lower
the pipetting head down onto the tip box until the Tip load button (4) flashes. When prompted to push the Tip load button, first press OK (14
) to switch to “low power” loading mode. Then push Tip load button while pushing down the Control unit until the Tip load button lights up.
Move the Pipetting unit (3
) up until the light of the Tip load button turns off.
4.2.3 Ejecting used GripTips
If liquid is in the tips, empty them by pressing the PURGE button (16 by pressing the Tip ejector (18
). Confirm ejection by pressing the Tip ejector a second
). Tips are ejected
time.
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4.3 Start pipetting

4.3.1 Pipetting
Use the Touch wheel (13 Actions you are about to perform will be displayed on the Run screen.
) to scroll to your desired pipetting mode and press OK (14).
Insert the tips into the liquid to be transferred. Press and release the RUN key (17) to aspirate the volume selected in the first step of your protocol (shown on the Run screen).
To execute subsequent steps, press the RUN key.
For a detailed description of all pipetting modes see “5.2 modes” on page 32. You can change the parameters of your pipetting mode at any time, see the following sections.
4.3.2 Set Z-Position
To define the optimal tip immersion depth, press or to access the Z-position screen.Then move the pipetting unit to the target position (A, B) until the actual Z-height and the current setting are displayed.
• Move the unit down to the desired dispensing height, e.g. 31 mm. Select Set to enter this position and save your setting.
• Repeat this procedure with position B.
4.3.3 Blowout modes
During the last dispense of a program, a blowout is performed automatically to ensure efficient liquid delivery. Liquid may be aspirated back into the tips when the pistons move back to the home position, a process called blowin. There are two ways to perform the blowin:
Automatic blowout: Pressing (and releasing) the RUN key starts the dispense with automatic blowout and blowin. You can choose a timed delay between the blowout and the blowin, see “Pipetting - Delayed blowin” under “3.4.4
Two-step automatic blowout: Perform a two-step blowout to manually delay the blowin:
- Press and hold
- Remove the tips from the target vessel.
- Release the RUN key to start blowin.
the RUN key to start dispense with blowout.
Detailed description of pipetting
Preferences” on page 16.
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VIAFLO 96/384 – Operating instructions V11 Operation
4.3.4 Recommendations for pipetting
INTEGRA Biosciences recommends the following techniques for enhancing pipetting results. These techniques are consistent with ISO standard 8655-2.
• It is best to immerse the GripTips just enough in liquid to allow the desired volume to be aspirated.
• Always pre-wet GripTips. After loading tips onto your pipette, aspirate and dispense the full volume 2-3 times to coat the inside of pipet tips. Pre-wetting ensures that the liquid and air inside the tips are at equal temperature and the dead air space is humidified.
• VIAFLO 96/384 is an air displacement pipette. It requires to touch the GripTips against the side of the well or dip them into the liquid after a dispense. This process is referred to as “touching off” or “tip touch” and prevents liquid from clinging to the pipette tips.
• In programs such as Repeat Dispense, a first and last dispense can be programmed. These two dispenses are not used and are dispensed into the waste as they contain the accumulated pipetting errors. Using a first and last dispense is recommended if accuracy and precision are of high importance.
• Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting. In addition, the pipetting mode “Reverse pipet” can be used to optimize pipetting results with viscous samples.
• For pipetting liquids with high vapor pressures (such as methanol or ethanol), use relatively fast pipetting speeds and avoid prolonged pauses after aspiration.
• Calibrate based on fluid type. VIAFLO 96/384 is tested and calibrated at the factory for use with distilled water at room temperature. It may be necessary to re-calibrate your VIAFLO 96/384 if the liquid to be used has different physical properties (specific gravity and vapor pressure) than water. Calibration mode can be accessed in the Toolbox menu.
ARNING
W
Avoid pipetting for extended periods. To minimize the risk of repetitive strain injury, include regular pauses of several minutes.
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4.4 Pipetting options and settings

4.4.1 Edit option
The Edit option is available for each mode. It enables you to access the variables that you can adjust for a pipetting mode. These variables include Speed, Volume, Pace, Count, Mix Cycles, Rows and Direction. Additional steps include First Dispense, Last Dispense, Air Gap, Aspirate Speed, Dispense Speed, etc..
Select a pipetting mode. Then, select Edit on the list of options and press OK. A list of associated steps is displayed. For example, if selecting Edit on the Repeat Dispense screen, the modifiable steps associated with Repeat Dispense are displayed.
4.4.2 Volume selection
To change a volume select the Edit option and press OK. The adjustable volumes are displayed.
Use the To u ch wheel to highlight the volume you want to change (Aspirate, Dispense, Mix, or Air Gap).
Press OK and a Volume setting “dial” is displayed. Use the Touch wheel to change the volume. Press
OK to confirm your volume selection and to save.
OTE
N
Use the arrow buttons to change the volume in coarse or fine incre ments. Select Coarse to change the volu me in larger increments. Select F ine to change the volume in smaller increments. The increment sizes vary based on the pipetting head, as shown under “7.4 page 54.
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Pipetting specifications” on
VIAFLO 96/384 – Operating instructions V11 Operation
Define and select favorite volumes
You can define, save, and select up to ten favorite volumes for quick access. These volumes can only be within the pipetting head volume range.
There are two ways to access and customize the list of favorite volumes:
• When in Pipet mode, use the Touch wheel to highlight Favorites and press OK.
• When in other modes, select the Edit option and press OK. The steps with volumes to be adjusted are displayed. Use the Touch wheel to highlight the desired volume and press Favorites to display the list of favorite volumes.
Use the Touch wheel to highlight the desired volume and press Select. Alternatively, modify a volume by pressing Edit.
Save your setting .
4.4.3 Speed selection
The speed option controls the speed at which liquid is aspirated, dispensed, or mixed in each mode. Speed can be set as a value from 1 (slowest) to 10 (fastest).
When in any pipetting mode, use the Touch wheel to highlight the Speed option and press OK. Select the speed and press OK to save your setting.
Speed may be changed in most Edit menus. Scroll to the Speed and press OK. Choose the speed, press OK, and press to save your selection.
The speeds selected in each mode (i.e., Pipet, Repeat Dispense, etc.) are stored for that mode only.
Speeds can be set independently for each operation (Aspirate, Dispense, Mix).
OTE
N
Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting. To dispense liquids with low viscosity and high vapor pressure, such as ethanol, use relative fast pipetting speeds and avoid prolonged pauses for aspiration.
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4.4.4 Custom
You can convert any predefined pipetting mode into a Custom program.
After setting up a pipetting mode with your parameters, use the Touch wheel to select
Custom.
You are prompted to enter an name. Use the To uch Wheel to select characters and press OK. Once
finished, press to save the name.
The program is now stored in the Custom program section.
4.4.5 Pace
The Pace option sets the time gap between dispenses in repeat pipetting. Pace is used in the Repeat Dispense and Variable Dispense modes. While you press and hold the RUN key, the pipette will dispense multiple programmed volumes with the selected pace. Release the RUN key to stop the paced dispense. Press the RUN key to continue dispensing.
Use the Touch wheel to select the desired Pace option and press OK. Select the pace, from None, 1 (slowest) to 9 (fastest). Press OK to save your setting.
4.4.6 Count, Mix Cycle and Rows
The Count, Mix Cycle, and Rows steps are used in various modes, see “5.2 description of pipetting modes” on page 32. Each is accessed with the Edit option. Use the Touch wheel to highlight the step and press OK.
Count sets the number of dispensing steps. Mix Cycle sets the number of mixes. In serial dilution mode, Rows sets the number of rows. A row indicator will notify the number of dilutions performed. Rows (first number) and Mix Cycles (second number) are tracked on the display. Mix Cycles are shown in red when mixing. A green dot on the row number indicates the active program step.
Select a desired value. Press OK and then press to save your setting(s).
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Detailed
VIAFLO 96/384 – Operating instructions V11 Operation
384
384
96
4.4.7 Pipetting between 96 and 384 well plates
For fast and simple reformatting between 96 and 384 well microplates and for loading of
12.5 or 125 µl GripTips with a 96-channel pipetting head, some Plate holders feature a
slide function to shift the microplate in Y-direction beneath the pipetting head. Put this
Plate holder, accommodating the 384 well plate, either on Position A or B (8). Move the Plate slider (9
) to one of the following 3 positions:
The back position of the Plate slider is used to accommodate the front positions of 384 well plates.
For pipetting the second columns move the pipetting head one well to the right, or left respectively.
The front position of the Plate slider accommodates the back positions of 384 well plates.
For pipetting the second columns move the pipetting head one well to the right, or left respectively.
Set the Plate slider to the middle to pipet 96 well plates.
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VIAFLO 96/384 – Operating instructions V11 Operation
a
4.4.8 Pipetting with the three position stage
The three position stage (see “8.1 of three possible positions.
Use the Stage slider (a) to accommodate the positions of 384 or 96 well plates as described in 4.4.7
.
Accessories” on page 55) supports the pipetting on two
Move the three position stage to the right to pipette on position A and AB.
Move the three position stage to the left to pipette on position AB and B.
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VIAFLO 96/384 – Operating instructions V11 Operation

4.5 Troubleshooting/FAQ

Problem Probable cause Remedy
Base unit is drifting sidewards.
Touch wheel does not work properly.
Menu options not selectable (grayed out).
Pipetting not possi­ble.
Tips cannot be loaded.
Tips are not cen­tered in the wells, although tip align­ment is activated.
Droplets on the tips. Temperature of liquid differs
Obstruction error. Base unit moved in X direc-
Control unit was touched at switch on.
Control unit was touched during homing.
No pipetting head installed. Install a pipetting head.
Side cover missing or pipet­ting head not correctly installed.
Z height defined too high above the top of the box.
Head alignment was not per­formed yet.
from that of air inside the tips. Liquid of low viscosity and
high vapor pressure. Touch-off was not performed. Perform a touch-off (mandatory in
tion by hand. Tips hit an obstacle during
X movement, e.g. box, con­tainer.
Guide rail soiled. Clean the guide rail for base unit. Other causes. Switch the device off and on.
Do not touch the control unit when switching on the VIAFLO 96/384. Restart VIAFLO 96/384 to initiate new homing routine.
Do not touch the control unit during homing. Restart VIAFLO 96/384 to initiate new homing routine.
Ensure pipetting head is installed correctly. Mount the side cover and screw the knob tightly.
Exit the pipetting mode. Alterna­tively clear Z height setting on position where tips are loaded.
Perform head alignment for every pipetting head.
Pre-wet tips up to 3 times.
Pre-wet tips and increase dispens­ing speed.
Repeat Dispense and Variable Dispense mode).
Always hold the control unit to move the base unit sidewards.
Move the tips upwards to a height that clears the tip rack out.
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5 Pipetting modes

This chapter describes how to program the VIAFLO 96/384 in two ways:
Function-based pipetting modes: You can select from ten predefined pipetting modes that you can quickly and easily edit and execute. They are described in the following sections.
Custom step-based programming mode: You can create and store up to forty multi­stepped pipetting protocols on the pipette using the basic functions of “Aspirate, Dispense, Mix, Purge, Prompt, Set Z Height, Tip Align and Loop” presented in “5.2
Detailed description of pipetting modes” on page 32. The custom programming
mode is described in “5.3

5.1 Overview pipetting modes

The table below provides an overview of the selectable pipetting modes. All modes are accessed from the Main Menu. Use the Touch wheel to scroll to your desired pipetting mode.
Pipetting mode Description
Pipet Allows liquid transfers when aspirate and dispense volumes are
Repeat Dispense Allows dispensing multiple aliquots of the same volume without
Sample Dilute Allows aspirating of sample and diluent divided by a defined air
Pipet/Mix Transfers a defined volume and follows with a defined number of
Manual Pipet Allows the operator to manually control the aspiration and
Reverse Pipet Allows liquid transfers of viscous or high vapor pressure liquids
Variable Dispense Allows dispensing multiple aliquots of different volumes. Variable Aspirate Allows aspirating multiple aliquots of different volumes. Sample Dilute/Mix Allows aspirating two liquids separated by an air gap followed by
Serial Dilution Allows aspirating a transfer volume followed by a mix. Rows and
Custom Allows to create and store of up to 40 multi-stepped pipetting
Press the OK to access the pipetting mode and to start defining parameters. Press to select the Help option.
Custom step-based programming mode” on page 41
equal.
refilling the tips after each dispense for fast microplate filling and processing.
gap into one tip, followed by a complete dispense.
automatic mixing cycles.
dispensing up to the set volume.
by preventing introduction of any air into the sample. The aspiration volume is higher than the volume to be dispensed.
a complete dispense and Mix step.
Mix Cycles are tracked on the display.
protocols.
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5.2 Detailed description of pipetting modes

VIAFLO 96/384 offers ten predefined pipetting modes. Most liquid handling protocols can be easily accommodated using one or more of these modes. The options and steps of the different pipetting modes are described in the following subsections.
5.2.1 Pipet mode
Application: Use this mode for quick transfer of liquid to or from microplates.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is equal to the dispense
volume. Speed Sets speed for the current pipetting step (1 = low, 10 = fast). Favorites Defines up to 10 favorite volumes Custom Converts the predefined program into a custom program.
Operation:
• With the tip(s) in liquid, press and release the RUN key to aspirate.
• With the tip(s) in the destination plate, press and hold the RUN key to execute the dispense and perform a two-step blowout, see “4.3.3
• When the tips are removed from the target plate, release the RUN key.
Blowout modes” on page 23.
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5.2.2 Repeat dispense mode
Application: This mode can be used for fast reagent addition to microplates from one
source container. You can dispense a large aspirated volume of liquid in multiple aliquots to multiple targets.
Options Steps Description
Edit Dispense Sets the volume for repetitive dispensing. The aspirated
volume is calculated automatically.
First Dispense
Last Dispense A last-dispense volume can be selected independently to
Reuse Last Disp.
Count The maximum number of dispenses possible (count) is
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast). Speed Sets speed of the current pipetting step. Pace Sets the time duration between dispenses in repeat pipetting,
Custom Converts the predefined program into a custom program.
Operation:
• With the tip(s) in liquid, press and release the RUN key to initiate the aspirate step.
• Press and release the RUN key for every dispense. Alternatively, press and hold Run to execute paced dispenses. The dispense number is shown on the display.
• The pipette will stop paced dispenses when it reaches the Last Dispense. You can choose to use this Last Dispense or discard it.
- If reuse of last dispense is not activated, press and hold the RUN key to purge the Last Dispense volume with a two-step blowout.
- If reuse of last dispense is active, you can start the next repeat dispense cycle with aspirating liquid to the last dispense in the tip. To finish the repeat dispense cycle, press PURGE.
A pre-dispense volume can be selected independently to improve accuracy and precision. The dispense is discarded.
improve accuracy and precision. The dispense is discarded. By default (red ), the mode ends with dispensing of the last
dispense. This aliquot contains the accumulated error from all prior dispenses. If you want to reuse the last dispense, press OK (green ). At the end of the program the last dispense remains in the tip, while the pipette is ready to aspirate a new volume to start the next repeat dispense run.
calculated automatically. This count may be reduced to the desired number.
if keeping RUN key pressed (1 = long, 9 = short).
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VIAFLO 96/384 – Operating instructions V11 Pipetting modes
5.2.3 Sample dilute mode Application: Accomplish accurate sample dilutions by using diluent to “chase” small
sample volumes from the pipet tips. An air gap keeps liquid separated in the tips and helps to minimize carryover of diluent when aspirating the sample.
Options Steps Description
Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip.
Air Gap Sets the volume of the air gap to keep both liquids separated. Aspirate 2 Sets the volume of the sample in the tip. Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast). Speed Sets speed of the current pipetting step. Custom Converts the predefined program into a custom program.
Operation:
• Press and release the RUN key to initiate each aspiration (remove tips from liquid for air-gap aspiration).
• Press and hold the RUN key to perform a two-step blowout. The entire tip contents will be dispensed together.
5.2.4 Pipet/mix mode
Application: Use this mode when mixing is required immediately after transfer of liquid.
This mode saves a programming step by incorporating the mix option after dispensing.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is equal to the dispense
volume. Mix Sets the mixing volume after dispensing. Mix Cycles Sets the number of mix cycles. Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast). Mix Speed Sets speed uniquely for mixing (1 = slow, 10 = fast).
Speed Sets speed of the current pipetting step. Custom Converts the predefined program into a custom program.
Operation:
• Press and release the RUN key to initiate the aspiration.
• Press and release the RUN key to dispense. Mixing occurs automatically after the dispense step.
• Upon completing the desired number of mixes, a blowout is initiated automatically prompting you to remove the tip(s) from the liquid and press the RUN key to complete the blowout.
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5.2.5 Manual pipet mode
Application: This mode can be used when the aspiration volume is not defined or
unknown. You have control over the aspiration and dispense steps and can view the display to confirm how much liquid has been aspirated or dispensed. Manual control over the dispense steps is perfect for performing titrations or for controlling the loading of samples in gel lanes.
Options Steps Description
Edit Aspirate Sets the aspiration or dispensing volume. Toggle between
Aspirate and Dispense using the Direction menu option. Speed Sets speed of the current pipetting step (1 = slow, 10 = fast). Direction Changes the direction of pipetting between aspiration ()
Favorites Defines up to 10 favorite volumes
Operation:
• When aspirating, the motor will stop when you release the RUN key or when the programmed aspirate volume is reached.
• You can change pipetting direction at any time even if aspiration volume is not reached. Change the direction of pipetting by pressing OK on the Direction option. The notation on the display changes between (Aspirate) and (Dispense).
• The volume remaining in the tip(s) is displayed.
N
OTE
Use slower pipetting speeds (1-5) for better control and resolution.
and dispensing ().
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VIAFLO 96/384 – Operating instructions V11 Pipetting modes
5.2.6 Reverse pipet mode
Application: With this mode the aspiration volume is higher than the volume dispensed.
It is recommended for liquid transfers of viscous and high vapor pressure liquids. The dispense method prevents introduction of air into the sample because no blowout is made.
Options Steps Description
Edit Dispense Sets the dispense volume.
Last Dispense Sets the volume to leave in the tip until final blowout. Reuse Last
Disp.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast). Speed Sets speed of the current pipetting step. Custom Converts the predefined program into a custom program.
Operation:
• Press and release the RUN key to initiate the aspiration. The total volume aspirated is the sum of desired dispense volume and last dispense volume.
• Press and release the RUN key to dispense the programmed volume.
• If reuse of last dispense is not activated, press and hold the RUN key to purge the Last Dispense volume with a two-step blowout.
• If reuse of last dispense is active, you can start the next reverse pipet cycle with aspirating liquid to the last dispense in the tip. To finish the reverse pipet cycle, press PURGE.
By (red ) default, the mode ends with dispensing of the last dispense. If you want to reuse the last dispense, press OK (green ). At the end of the program the last dispense remains in the tip, while the pipette is ready to aspirate a new volume to start the next reverse pipet run.
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5.2.7 Variable dispense mode
Application: Use this mode when differing dispense volumes are required. This mode
could be used to quickly set up a dilution series in plates or for feeding similar samples to different assay plates where different sample volumes are needed.
Options Steps Description
Edit Count Sets the total number of dispensing steps.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast). Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast). Dispense
1...Count
Speed Sets speed of the current pipetting step. Pace Sets the time interval between dispenses, if keeping RUN
Custom Converts the predefined program into a custom program.
Operation:
• Press and release the RUN key to initiate the aspiration of total volume.
• Press and release the RUN key to initiate each subsequent dispense. The pipette stops and beeps when ready for the Last Dispense step, i.e. to purge the calculated waste volume amount.
• Alternatively, press and hold the RUN key to execute paced dispenses. The pipette stops paced dispensing when it reaches the Last Dispense. This aliquot contains the accumulated error from all prior dispenses. You can choose to use this Last Dispense or discard it.
• During the Last dispense, press and hold the RUN key to perform a two-step blowout.
Sets different volumes for repeated dispensing. Number of dispenses and dispense volume cannot exceed nominal volume of the pipetting head. The total volume is automatically calculated.
key pressed (1 = long, 9 = short).
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VIAFLO 96/384 – Operating instructions V11 Pipetting modes
5.2.8 Variable aspirate mode
Application: This mode can be used for a variety of collection applications where the
aspiration volume is well known. This mode is also suited for supernatant collection in microplates.
Options Steps Description
Edit Count Sets the total number of aspirating steps.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast). Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast). Aspirate
1...Count
Speed Sets speed of the current pipetting step. Custom Converts the predefined program into a custom program.
Operation:
• With the tip(s) in liquid, press and release the RUN key to initiate the first aspiration volume. Again in liquid, press and release the RUN key to initiate the second aspiration volume, etc.
Press and hold the RUN key to start Dispense and perform a two-step blowout.
Sets different volumes used for sequentially aspirating (in the same tip) followed by a single dispense. Number of aspirates and aspirate volume cannot exceed nominal volume of the pipetting head.
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5.2.9 Sample dilute/mix mode
Application: Use this mode to perform sample dilutions where mixing of sample and
diluent is required. This mode could also be used to introduce and mix diluent and sample to the first column of a serial dilution plate.
Options Steps Description
Edit Aspirate 1 Sets the volume of the sample aspirated first in the tip.
Air Gap Sets the volume of the air gap to keep both liquids separated. Aspirate 2 Sets the volume of the diluent in the tip. Mix Sets the mixing volume after dispensing. Mix Cycles Sets the number of mix cycles. Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Mix Speed Sets speed uniquely for mixing (1 = slow, 10 = fast). Speed Sets speed of the current pipetting step. Custom Converts the predefined program into a custom program.
Operation:
• With the tip(s) in liquid, press and release the RUN key to initiate aspiration 1. With the tip(s) out of the liquid, press and release the RUN key for the Air Gap. Again in liquid, press and release the RUN key to initiate aspiration 2.
• Press and release the RUN key to dispense the entire tip contents and begin the mixing routine. Upon completing the desired number of mixes, a blowout occurs automatically. Remove tips from liquid and press and release the RUN key to complete the blowout.
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VIAFLO 96/384 – Operating instructions V11 Pipetting modes
5.2.10 Serial dilution mode
Application: Use this mode to perform serial dilutions. The Serial Dilution mode enables
aspiration of a specific volume followed by a mix sequence and ending with the original aspiration volume in the tips.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is identical to the dispense
volume. Mix Sets the mixing volume after dispensing. Mix Cycles Sets the number of mix cycles. Rows Sets the number of rows. A row indicator will notify the
number of dilutions performed. Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast). Mix Speed Sets speed uniquely for mixing (1 = slow, 10 = fast). Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast).
Speed Sets speed of the current pipetting step. Custom Converts the predefined program into a custom program.
OTE
N
For serial dilutions it is helpful to switch Column Detent on, see “Tip Align” under "
Position Settings" on page 16.
Operation:
• Attach 8/16 GripTips on the leftmost row by lowering the Pipetting unit onto the tips.
CAUTION
When prompted to push the Tip load button, first press OK (14) to switch to “low power” loading mode. Then press the Tip load button and at the same time push down the Control unit to load the tips with reduced forces.
• Replace the tip box with the microplate and put the reagent container on the other position, e. g. position A. Press and release the RUN key to initiate the aspiration of the reagent.
• Press and release the RUN key to start the dispense and mix sequence. Proceed with the next rows.
• Rows (first number) and Mix Cycles (second number) are tracked on the display. Mix Cycles are shown in red when mixing. A green dot on the column number indicates the active program step.
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5.3 Custom step-based programming mode

Application: Use the Custom program mode to create personalized pipetting protocols.
Up to forty programs can be stored. Select “Custom” to create a personalized protocol. Programs can contain up to 98
individual steps based upon the following basic operations: Aspirate, Dispense, Mix, Purge, Prompt, Set Z Height, Tip Align and Loop.
Press to create a new program. Select the pipetting head and the type of the new custom program, i. e. Manual or Automatic.
OTE
N
The program type can only be selected if “Automatic” is activated under Toolbox - Preferences - Main Menu, see “3.4.4 We recommend creating automatic custom programs on a PC with the VIALINK software, see also “5.4 VIALINK” on page 46.
Next, your are prompted to enter a name. Use the Touch Wheel to select characters and press
OK. Once finished, press to save the name.
Preferences” on page 16.
The first step is highlighted, press OK. Use the Touch Wheel to select the first step from the
menu, e. g. Aspirate. Press OK. Define the required parameters and press OK to add the step.
After adding the first step, the selection should now be on the second line. Press OK again to define the second step. Continue adding steps until your entire pipetting protocol is defined.
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VIAFLO 96/384 – Operating instructions V11 Pipetting modes
5.3.1 Manual custom program
Manual custom programs can consist of the following steps:
Step Description
Aspirate Sets an aspiration volume and speed. Dispense Sets a dispense volume and speed. Mix Sets the mixing volume after dispensing. Purge Purges all remaining liquid currently in the GripTips with the selected
Prompt A prompt is any message that is displayed during the program. Three
Z Height Sets the Z height on Position A or B. Tip Align Within a custom program, you can switch the tip alignment on or off,
Loop A loop repeats the steps between the selected step and the loop
purge speed. A “Purge” step is automatically integrated at the end of a program if the last programming step leaves liquid in the tips.
lines with a total of 30 characters are available. To continue the program, press the RUN key.
if required. Use the Touch wheel to select Tip Align and press OK.
„“ (
Select Position A or B and pr ess OK to toggle between „“
(Off), see also “3.4.4 Preferences” on page 16.
The tip align setting applies to the subsequent steps of the custom program.
command. Chose to which step the loop directs and how many times the loop
should be performed. The number of steps can often be shortened by adding a loop. Note: Nested loops (loops inside loops) are not allowed.
On) and
When starting with an “Aspirate” step followed by a “Mix” step, the tips contain the aspirate volume after completing the last mix cycle. When starting with a “Mix” step, the tips are emptied upon completion of the last mixing cycle.
To save and store a Custom program, press Save.
42 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Pipetting modes
Example of manual custom program
The task is to combine 2 different liquids in a 96 well plate for a kinetic assay and then mixing it to achieve a homogeneous solution. The microplate should then be incubated for 5 minutes and the content is then distributed to a 384 well plate. The custom program would be set up as followed:
Program step Action
1) Aspirate liquid 1: 160 µl (e.g. diluent) With tips in liquid 1 press RUN key.
2) Aspirate air: 20 µl Move tips out of liquid and press RUN key.
3) Aspirate liquid 2: 50 µl (e.g. reagent) With the tips in liquid 2 press RUN key.
4) Dispense: 230 µl Press and hold RUN key until liquid is
dispensed and tips are removed from the liquid (two-step blowout).
5) Mix 3x: 200 µl Press RUN key.
6) Prompt: Incubate 5 min No action.
7) Aspirate: 210 µl With tips in the 96 well plate, press RUN key.
8) Dispense: 50 µl Move the Plate slider to the front and press
9) Dispense: 50 µl Move the pipetting head one well to the right
10) Dispense: 50 µl Move the Plate slider to the back and press
11) Dispense: 50 µl Move the pipetting head to the left and press
12) Purge Purge (does not need to be programmed).
RUN key to dispense the back positions of the 384 well plate.
and press RUN key to dispense the second columns.
RUN key to dispense the front positions of the 384 well plate.
RUN key to dispense the first columns.
The residual liquid is dispensed into the waste container. Press and hold RUN key until liquid is purged and tips are removed from the liquid (two-step blowout), see “4.3.3 Blowout modes” on page 23.
N
OTE
The program steps 8-11 can be shortened with the Loop step:
9) Loop to step 8, 4 times
10) Purge
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VIAFLO 96/384 – Operating instructions V11 Pipetting modes
5.3.2 Modify existing programs
At the Custom program display, use the Touch wheel to highlight an existing program. Press  Options, use the Touch wheel to select an option (View/Edit, Delete, Copy, Rename) to modify the program and press OK
OK
Press Back to return to the list of Custom programs. To run the program, press
5.3.3 Automatic custom program
The Automatic Mode performs automatically a defined series of liquid handling operations. This mode is by default inactive and has to be manually enabled, see “3.4.4 Preferences” on page 16.
An automatic Program always starts with a Move X,Z step to define the starting position.
Step Description
Move X,Z Teach the unit by moving the pipetting unit to the desired position.
Current Setting is the position to be used by the program, X and Z indicates the momentary position. Move the pipetting head to the desired position and press Set to adapt the Current Setting to the momentary position.
Move Z Move the pipetting head to the desired position and press OK to
adapt the Current Setting to the Actual Position. The pipetting unit moves to the defined absolute Z height.
Move X This command travels the set distance in X-direction relative to the
actual position. Setting a negative value (mm) moves the unit to the left, setting a positive value (mm) moves the unit to the right.
Use the Touch wheel to enter the desired value and press OK.
.
AUTION
C
Always ensure that the pipetting head is at a clear height for a movement to the side.
44 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Pipetting modes
Step Description
Blowout / Blowin In automatic mode, the blowout and blowin need to be programmed
after the last dispense. After a blowout, a bl owin has to fo llow a t some po int. It does not have
to follow immediately and can have steps in between. E.g. after the blowout a move step can be programmed to move the tips out of the liquid, and is then followed by the blowin.
Note: When using “Purge” to empty the tips, a blowout/blowin is performed automatically and does not need to be programmed.
Delay A delay is a pause between the last and the next step.
If it is set to 0.0 s, pressing the run button is required to trigger the next step.
Loop A loop repeats the steps between the selected step and the loop
command. Chose to which step the loop directs and how many times the loop
should be performed. The number of steps can often be shortened by adding a loop. Note: Nested loops (loops inside loops) are not allowed.
Tip Change Pauses the program. The user is prompted to eject and load tips
manually. The program continues with the next step after pressing the RUN key.
As manual custom programs, automatic programs can be created directly on VIAFLO 96/
384. However, we recommend using the VIALINK software to set up automatic custom
programs, see “5.4 VIALINK” on page 46.
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VIAFLO 96/384 – Operating instructions V11 Pipetting modes
Example of automatic custom program
The task is to perform a serial dilution over the entire 96 well plate. The concentrated sample is in column 1. The custom program would be set up as followed (note that position values might slightly differ when adapting the protocol on a different unit):
Program step Action
1) Move X,Z: X -16.6 mm, Z 30,1 mm Sets the start position to aspirate the sample
2) Aspirate liquid: 100 µl The concentrated sample is aspirated.
3) Mix: 2x 100 µl The sample is re-suspended during mixing.
4) Move Z: 40.8 mm The pipetting head moves upwards.
5) Move X: 9.0 mm The pipetting head moves to the next column
6) Move Z: 30.0 mm The pipetting head moves down into the
7) Mix 3x: 150 µl During mixing the sample is diluted, 100 µl
8) Loop: Step 5, Count 11 Continue serial dilution in the next wells
9) Move Z: 80.7 mm The pipetting head moves upwards.
10) Move X,Z: X -75.8 mm, Z 52.6 mm The pipetting head moves to the waste
11) Purge: Speed 8 The residual liquid is dispensed into the
in the first column.
The aspiration volume of 100 µl remains in the tips after mixing.
on the right.
well.
remain in the tips after mixing.
(repeats steps 5-8 eleven times).
container.
waste container.
N
OTE
GripTips cannot be attached automatically.

5.4 VIALINK

VIALINK is a pipette management software for the PC and is used to manage VIAFLO 96/ 384 and VIAFLO electronic hand held pipettes. It allows to create custom programs, manage a library of custom programs, establish a service history and more.
To establish a connection between computer and VIAFLO 96/384, a standard USB cable (type A to B) is required.
The software VIALINK can be downloaded from the INTEGRA website in the product section and is free of charge for all VIAFLO 96/384 customers. A detailed description of the software, along with the operating instructions, can be found on the website as well.
46 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Maintenance

6 Maintenance

6.1 Cleaning and Decontamination

WARNING
Always turn off power and disconnect the VIAFLO 96/384 from the mains when carrying out maintenance work.
6.1.1 Base unit
If the external components get soiled, clean them with a lint-free cloth lightly soaked with mild soap solution in distilled water or with a 70 % dilution of Isopropyl or Ethanol. Never use acetone or other solvents. Ensure that the guide rails of the base unit are clean, greasing is not required.
If the surfaces of the VIAFLO 96/384 have been in contact with biohazardous material, they must be decontaminated in accordance to good laboratory practice. Wipe the clean surface with a lint-free cloth, lightly soaked with a disinfectant. For example:
• Ethanol 70 %
• Microcide SQ 1:64
• Glutaraldehyde solution 4 %
• Virkon solution 1-3%
Follow the instructions provided with the reagents.
6.1.2 Pipetting heads
Remove the pipetting head from the device. Regularly clean the pipetting head surface and the tip fittings with a moist lint-free cloth lightly soaked in distilled water or Ethanol/ Isopropyl 70 %. Alternatively, just spray Ethanol 70% or Isopropyl 70% on the tip fittings and let them dry. Do not spray into the tip fittings.
ARNING
W
Spraying Ethanol 70% or Isopropyl 70% on the tip fittings may not be sufficient to completely decontaminate the pipetting heads because it does not reach the inner parts. Only gas sterilization or autoclaving inactivates harmful micro-organisms on the inner parts of the pipetting heads.

6.2 Prolonged period of non-use

Pipetting heads of VIAFLO 96/384 should not be left inside the device if not used for extended periods (>1 week). Remove the pipetting head from the device and store it wrapped in a plastic bag in the appropriate casing.
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VIAFLO 96/384 – Operating instructions V11 Maintenance

6.3 Leak test

It is recommended to perform a leak test every 3 months or when errors occur. Leak test may be done on half of the head at a time or column by column for a better view
of the liquid levels (load one GripTip column with reduced force as described under operation of “4.2.2
1) Choose Pipet/Mix mode and enter the following settings: aspirate the full volume at
speed 6, mix cycles 6 at speed 6.
2) Fill a reservoir with distilled water (add some food coloring for better visibility).
3) Pre-wet the GripTips: Start the Pipet/Mix program by pressing the RUN key (17
aspiration, press the PURGE button (16 Repeat 2 more times to a total of 3 pre-wet cycles.
4) Press RUN again to aspirate. Leave the tips immersed 2-3 mm in the liquid and
observe for 30 s the liquid levels in the tips. During this wait time the liquid levels must not decrease.
5) Press RUN again to start the mix cycles.
a) Observe whether air bubbles are forming at the tip ends when dispensing. b) Check whether the levels stay approximately the same across all channels. c) At the end of a last dispense it is normal to have air bubbles because a blowout is
performed.
If in doubt about a particular channel, load a tip on this channel only and repeat the test.
Signs indicating a leak
1) Liquid level in one or more tips is decreasing while the tips are immersed in liquid
during the 30 s wait time.
2) During the mix cycle the top aspiration level in one or more tips is gradually
decreasing. This shows in uneven liquid levels across the tips after aspiration.
3) One or more tips show air bubbles at the tip end during the mix cycles.
Partial tip loading” on page 22).
). After
) and confirm with RUN. This empties the tips.
OTE
N
A decreasing liquid level at aspiration could be an indication of a slow leak. Performing a retest at 10 mixes may help identify a slow leak.
If a leaking channel is identified, change the red O-ring on that specific tip fitting (where applicable, see 6.4.2) or contact your service technician.
48 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Maintenance

6.4 Servicing

WARNING
If liquid ever enters the internals of VIAFLO 96/384 of the pipetting heads, please contact your service technician.
Pipetting heads need to be serviced by INTEGRA in the following cases:
• Liquid has entered the pipetting head
• The pipetting head had to be autoclaved for decontamination
• One or more channels did not pass the leak test
• The pipetting head was damaged
ARNING
W
If working with infectious materials, e. g. human pathogens, the pipetting heads need to be gas sterilized or autoclaved to decontaminate before sending them to service. The declaration on the absence of health hazards must be signed to confirm that the equipment has not been exposed to biohazards or radioactive materials and was app ropriately decontaminated. This is necessary to protect service personnel.
6.4.1 Blocked pipetting unit
If the control unit is defect or the pipetting unit parks in down position and the pipetting head can not be removed, start the following procedure:
1) Turn off the VIAFLO 96/384. Turn it back on while pressing the Tip load button (4
approx. 5 sec. until the Tip load button lights up permanently.
2) Release the Tip load button. The button flashes, the pipetting unit moves up and the
removal of the pipetting head will be enabled. (Ignore any display messages on the control unit.)
3) As soon as the button lights permanently, you can remove the pipetting head.
4) Press the Tip load button for approx. 5 sec. until the button flashes. The pipetting unit
moves into the park position.
5) When the light of the Tip load button is off, turn off the VIAFLO 96/384.
) for
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VIAFLO 96/384 – Operating instructions V11 Maintenance
a
b
6.4.2 Changing O-rings of tip fittings
300 µl and 1250 µl pipetting heads feature tip fittings with red O-rings to seal against the inside wall of GripTips providing optimal sealing. O-rings are made of durable silicone.
If necessary, e.g. in case of a leakage due to damaged O-rings, you can replace these O­rings. A set of spare O-rings and an O-ring removal tool are included with each 300 µl and 1250 µl pipetting head, but can also be ordered separately, see “8 consumables” on page 55.
WARNING
Avoid mechanical damage of the tip fittings.
Choose the side of the O-ring removal tool corresponding to the size of the pipetting head (300 µl or 1250 µl). Slide the O-Ring removal tool sidewards onto the tip fitting until the O-ring (a) builds a loop. Cut the O-ring with fine scissors and remove it.
Slide a new O-ring over the tip fitting (b).
Accessories and
50 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Maintenance

6.5 Calibration

VIAFLO 96/384 can be calibrated by adjusting a correction factor in the software. The correction factor is determined by a nominal volume and the measured volume. To assess the accuracy and precision of VIAFLO 96/384 pipetting heads, different methods can be used:
• Measure one or more channels gravimetrically and then all other channel by photo­metry. Compare the photometrically determined channels relative to the gravimetri­cally measured channels. Contact INTEGRA for a detailed protocol.
•Gravimetric only.
• Using commercially available systems (e.g. ARTEL MVS).
INTEGRA Biosciences offers a calibration service for VIAFLO 96/384 pipetting heads. Please contact your local dealer for more information.

6.6 Equipment disposal

The VIAFLO 96/384 is labeled with the “crossed-out wheeled bin” symbol to indicate that this device must not be disposed of with unsorted municipal waste. Instead, it is your responsibility to correctly dispose of your waste equipment by handing it over to an authorized facility for separate collection and recycling. It
is also your responsibility to decontaminate the device in case of biological, chemical, and/or radiological contamination so as to protect from health hazards the persons involved in the disposal and recycling of equipment.
For more information about where you can drop off your waste equipment for recycling, please contact your local dealer from whom you originally purchased the product or your local council.
By doing so, you will help conserve natural resources and you will ensure that your waste equipment is recycled in a manner that protects human health and the environment. Thank you!
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VIAFLO 96/384 – Operating instructions V11 Technical Data

7 Technical Data

7.1 Environmental conditions

Operation
Temperature range 5–40°C Humidity range Max. rel. humidity 80 % for temperatures up to
31 °C, decreasing linearly to 50 % rel. humidity at 40 °C.
Altitude range < 2000 m

7.2 Specification of the device

Dimensions (W x D x H) 42 cm x 30 cm x 54 cm (17” x 12“ x 21“) Weight of VIAFLO 96 incl.
pipetting head Weight of VIAFLO 384 incl.
pipetting head Power Requirements 100–240 VAC, 50/60 Hz, 450 W Pipetting channels of VIAFLO 96 96, individual Pipetting channels of VIAFLO 384 384, 96, individual Pipetting speed 10 steps Compatible plate formats 96 and 384 wells, shallow and deep well Plate positions up to 3 Pipetting technology Air displacement User interface Touch wheel, color display
25.7 kg (56.7 lbs)
27.7 kg (61.1 lbs)
52 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Technical Data

7.3 Intellectual Property

The VIAFLO 96/384 are covered under the following patents:
Patent Number Country Title Apply to
8,033,188 USA Pipettor Software Interface VIAFLO 96/384 2192985 EPC/
FRAN/ GBRI/ SWIT
5221661 JAPA Pipettor Software Interface VIAFLO 96/384
602008010945 GERM Pipettor Software Interface VIAFLO 96/384 8,367,022 USA Unintended Motion Control for Manu-
8,372,356 USA Manually Directed, Multi-Channel
8,468,900 USA Pipette Tip Positioning for Manually-
7,811,522 USA Sample Reservoir Kits With Dispos-
D599,031 USA A Liquid Sample Or Liquid Reagent
8,277,757 USA Pipette Tip Mounting Shaft GripTips 8,501,118 USA Disposable Pipette Tip GripTips
Pipettor Software Interface VIAFLO 96/384
VIAFLO 96/384 ally Directed, Multi-Channel Elec­tronic Pipettor
VIAFLO 96/384 Electronic Pipetting System
VIAFLO 96/384 Directed Multi-Channel Electronic Pipettor
Reservoirs able Liners
Reservoirs Reservoir Kit
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VIAFLO 96/384 – Operating instructions V11 Technical Data

7.4 Pipetting specifications

Head
Part No.
6101 6131
Vol ume
Range
(µl)
0.5–12.5 0.01
Min. Scrolling
Volu me
Increments (µl)
Test Volume Accuracy Precision
(µl) (±%) (≤%)
1.25 8.0 3.0
6.25 2.0 1.5
12.5 1.5 1.0
6106 6136
1
(1 µl–)
2–50 0.05
55.02.5 25 2.0 1.5 50 1.5 1.0
6102 6132
6103
6104
(2 µl–)
5–125
(5 µl–)
10– 300
(25 µl–) 50–1250
1
0.1
1
0.5
1
1
12.5 3.0 1.7
62.5 2.0 1.5 125 1.5 1.0
30 2.5 1.5 150 1.5 1.0 300 1.2 0.8 125 2.5 1.5 625 2.0 1.0
1250 1.8 0.8
1. The volumes in brackets refer to extended volumes, see “Pipetting” under “3.4.4 Preferences” on page 16. For the extended range these specifications do not apply.
54 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Accessories and consumables

8 Accessories and consumables

8.1 Accessories

Pipetting heads Volume range Part No.
96-channel pipetting heads
384-channel pipetting heads
General Part No.
PCR 96 well cooling block 6250 PCR 384 well cooling block 6255 O-ring removal tool (for 6103 and 6104) 130-00731-00
Plate holders Part No.
Standard plate holder (for 96 well plates) 6205 Plate holder with side function
(for 96 and 384 well plates, with 384 well offset) Spring loaded plate holder A with slide function
(for 96 and 384 well plates, with 384 well offset) Spring loaded plate holder B with slide function
(for 96 and 384 well plates, with 384 well offset) Row dilution plate holder (for 96 and 384 well plates) 6221 Adjustable spring loaded plate holder A with slide function
(for 96 and 384 well plates, with 384 well offset) Adjustable spring loaded plate holder B with slide function
(for 96 and 384 well plates, with 384 well offset) Adjustable spring loaded plate holder A with slide function
(for 96, 384 and 1536 well plates, with 1536 well offset) Adjustable spring loaded plate holder B with slide function
(for 96, 384 and 1536 well plates, with 1536 well offset) Three position stage (occupies both positions A and B,
for 96 and 384 well plates)
0.5–12.5 µl 6101 2–50 µl 6106 5–125 µl 6102 10–300 µl 6103 50–1250 µl 6104
0.5–12.5 µl 6131 2–50 µl
5–125 µl 6132
6136
6210
6215
6220
6223
6224
6225
6226
6230
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VIAFLO 96/384 – Operating instructions V11 Accessories and consumables

8.2 Consumables

GripTips for VIAFLO 96/384 Part No.
Purple:
12.5 µl GripTips
Yel low:
50/125 µl GripTips
Green:
300 µl GripTips
Blue:
1250 µl GripTips
5 XYZ racks of 384 tips, non-sterile, LONG 6403 5 XYZ racks of 384 tips, sterile, LONG 6404 5 XYZ racks of 384 tips, sterile, filter, LONG 6405 5 XYZ racks of 384 tips, sterile, filter, SHORT 6475 5 XYZ racks of 384 tips, non-sterile 6453 5 XYZ racks of 384 tips, sterile 6454 5 XYZ racks of 384 tips, sterile, filter 6455 5 XYZ racks of 384 tips, non-sterile 6463 5 XYZ racks of 384 tips, sterile 6464 5 XYZ racks of 384 tips, sterile, filter 6465 5 inserts of 96 tips, non-sterile, GREEN CHOICE 6432 5 racks of 96 tips, non-sterile 6433 5 racks of 96 tips, sterile 6434 5 racks of 96 tips, sterile, filter 6435 5 inserts of 96 tips, non-sterile, GREEN CHOICE 6442 5 racks of 96 tips, non-sterile 6443 5 racks of 96 tips, sterile 6444 5 racks of 96 tips, sterile, filter 6445
O-rings for tip fittings Part No.
300 μl Replacement kit 24 per pack 100-00027-50 300 μl Replacement kit 100 per pack 125928 1250 μl Replacement kit 24 per pack 100-00028-50 1250 μl Replacement kit 100 per pack 125929
56 INTEGRA Biosciences AG
VIAFLO 96/384 – Operating instructions V11 Accessories and consumables
Automation friendly disposable reagent reservoirs Part No.
150 ml 8 reservoir bases, automation friendly (microplate for-
mat), non-sterile 25 lids, sterile, polypropylene 6302 Trial pack (1 base, 1 reservoir and 1 lid), sterile 6303 30 reservoirs, individually sealed, sterile, polystyrene,
incl. 1 trial pack 100 reservoirs, 4 sleeves of 25 inserts, sterile,
polystyrene, incl. 1 trial pack
300 ml 8 reservoir bases, automation friendly (microplate
format), non-sterile 25 lids, sterile, polypropylene 6306 Trial pack (1 base, 1 reservoir and 1 lid), sterile 6307 30 reservoirs, individually sealed, sterile, polystyrene,
incl. 1 trial pack 100 reservoirs, 4 sleeves of 25 inserts, sterile,
polystyrene, incl. 1 trial pack
Automation friendly polypropylene reagent reservoirs Part No.
96 Well, 300 ml, 25 reservoirs per case, pyramid bottom, non-sterile 6351
25 reservoirs per case, pyramid bottom, pre-sterilized 6352
6301
6317
6318
6305
6327
6328
12 Column 25 reservoirs per case, pyramid bottom,
non-sterile 125 reservoirs per case, pyramid bottom,
pre-sterilized
8 Row 25 reservoirs per case, pyramid bottom, non-sterile 6371
25 reservoirs per case, pyramid bottom, pre-sterilized 6372
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6361
6362
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