Integra VACUSIP Operating Instructions Manual

VACUSIP Operating instructions

159950_V04

Declaration of conformity

INTEGRA Biosciences AG –

declares on its own responsibility that the devices

Description Models

VACUSIP 159000, 159010

comply with:

EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW

Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU

Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU

Restriction of Hazardous Substances 2011/65/EU

Waste Electrical and Electronic Equipment 2012/19/EU

Battery Directive 2006/66/EC

EU Regulations

Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)

Capacity Labelling of Portable Secondary Batteries 1103/2010

Ecodesign - Power supplies 278/2009

Standards for EU

Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.

Electrical equipment for measurement, control and laboratory use ­EMC requirements.

7205 Zizers, Switzerland

1907/2006

EN 61010-1: 2010

EN 61326-1: 2013

Standards for Canada and USA

Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.

Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.

Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including
interference that may cause undesired operation.

Zizers, November 4, 2016

Elmar Morscher
CEO

Thomas Neher
Quality Manager

CAN/CSA-C22.2

No. 61010-1

UL 61010-1

Part 15 of the

FCC Rules

Class A

VACUSIP - Operating instructions V04

Table of Contents

1 Introduction ................................................................................................................4

2 Description of the device ............................................................................................6

3 Installation ..................................................................................................................7

4 Operation ...................................................................................................................9

5 Maintenance .............................................................................................................11

6 Technical data ..........................................................................................................12

7 Accessories and consumables .................................................................................14

Imprint

© 2018 INTEGRA Biosciences AG

Manufacturer
INTEGRA BIOSCIENCES AG

CH-7205 Zizers, Suisse
T [phone]
F [phone]
[e-mail]
www.integra-biosciences.com

Customer service

Please contact your local INTEGRA Biosciences representative, see
www.integra-biosciences.com

or contact [e-mail].

INTEGRA BIOSCIENCES CORP.

Hudson, NH 03051, USA
T [phone]
F [phone]

English

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VACUSIP - Operating instructions V04 Introduction

1 Introduction

These operating instructions contain all the information required for installation, operation
and maintenance of the VACUSIP aspiration system.

English

1.1 Intended use

This is a general-purpose laboratory instrument. Any use of this instrument in a medical
or IVD setting is under the sole responsibility of the user.

VACUSIP is a vacuum-based system for aspiration of non explosive aqueous solutions,
such as biological buffers and media.

If the VA CUS IP is used i n a man ner not specified by INTEGRA Biosciences, the protection
provided by the VACUSIP may be impaired.

1.2 Safety notes

WARNING

Read these operating instructions carefully before use and pay particular
attention to sections containi ng thi s symb ol .

1.2.1 General

1) Do not carry out any conversions and alterations on the device.

2) Defective parts may only be replaced with original INTEGRA Biosciences spare parts
according to the INTEGRA Biosciences operating or service instructions.

3) Do not use or charge VACUSIP in an atmosphere with danger of explosion. Also, do
not aspirate highly flammable liquids such as acetone or ether.

4) When handling dangerous substances, comply with the material safety data sheet

(MSDS) and with all safety guidelines such as the use of protective clothing and safety
goggles.

5) Prolonged exposure of VACUSIP to UV-light can cause discoloration and/or yellowing

of the plastic housing. However, this will not affect the performance of the device in any
way.

6) Excess spare parts, accessories and consumables (e.g. tubings, lids, plastic parts,

rubber products, O-rings, filter) should be stored in a light protected place at ambient
temperature to prevent premature material aging caused by prolonged exposure to UV­light.

Regardless of the listed safety notes, additionally applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com
information regarding REACH classified chemicals contained in our products.

on a regular basis for up to date

4 INTEGRA Biosciences AG

VACUSIP - Operating instructions V04 Introduction

1.2.2 Battery (model 159 000)

1) Charge the VACUSIP device using only the charger supplied by INTEGRA
Biosciences.

2) Old Li-ion batteries may cause a safety risk. We recommend to replace the battery
after 3 years of use. Also replace the battery if the charging intervals are unusually
short or if the charging takes much longer than usual (6 hours or more). These are
indicators that the battery has reached the end of its life-cycle.

3) Li-ion technology bears the risk of thermal runaway and cell rupture if the battery was
damaged. Do not expose the battery to heat (> 60 °C) and avoid mechanical stress.
Batteries which were subject to deep discharges may develop internal short circuits,
leading to an increased self-discharge rate and heating during battery charging. This
may also result in thermal runaway and cell rupture.

4) To extend the battery life-cycle, it is recommended to charge the battery every 2
months if the VACUSIP is not used regularly. If the VACUSIP is not used for more than
6 months, unplug the battery, see 3.3.5

5) If despite all safety measures the VACUSIP Li-ion battery catches fire and toxic
(typically dense, white) fumes emanate from the VACUSIP device, immediately
evacuate the working area. The VACUSIP housing is made of special flame retardant
material that has proven its capability to contain a battery fire. During a battery fire the
housing surface may become very hot. Do not touch the housing by hand and wait at
least 20 minutes after fumes have disappeared before approaching the device. Do no
longer use the VACUSIP device.

.

English

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VACUSIP - Operating instructions V04 Description of the device

1

3

2

4

5

6

7

8

13

9

10

11

2 Description of the device

Scope of delivery

• VACUSIP base unit without battery (model 159 010) or with battery (model 159 000)

• Mains adapter

• INTEGRA glass bottle 500 ml with standard GL 45 lid

English

• GL 45 lid with tube fittings

• Tubing and hydrophobic filter with an additional spare filter

• VACUSIP hand operator

• 8-channel stainless steel tips

• 1-channel stainless steel tip

• 1-channel adapter for disposable tips

1 On/off switch

2 Indicator LED

3 Base unit

4 Stainless steel rod

5 VACUSIP hand operator

6 Hand operator mount

7 Lid with tube fittings

8 500 ml bottle for liquid collection

Rear view

12

9 Hydrophobic filter

10 Silicone filter adapter

11 Air outlet

12 Opening to insert stainless steel rod

6 INTEGRA Biosciences AG

13 Mains connection socket

VACUSIP - Operating instructions V04 Installation

3 Installation

3.1 Power supply

Insert the mains adapter cable into the VACUSIP mains connection socket (13) and plug
it into the power source.

ARNING

W

Use an original INTEGRA Biosciences mains adapter only (see “6.1
Specifications” on page 12 for voltage requirements)

3.2 Charging of VACUSIP battery model

A full charge takes 5 hours. Before the first use, VACUSIP should be charged for at least
5 hours.

When the LED (2
prolong battery life. VACUSIP has an integrated overcharge protection.

VACUSIP can be used while it is being charged.

3.3 Set-up

3.3.1 Installing the VACUSIP hand operator mount

Find the enclosed stainless steel rod (4) and push it into the opening (12).

Attach the hand operator mount (6

) starts to blink green, the battery needs to be recharged immediately to

) to the top of the stainless steel rod (4).

ARNING

W

Do not carry VACUSIP by holding the rod or the hand operator mount. Always
grab the instrument at the back to carry it around.

English

3.3.2 Assembling the hand operator

Insert the inner part in the silicone
cover, as shown on the picture
beside.

3.3.3 Connecting the filter

Attach the shorter silicone tubing to the filter (9) and then plug the filter into the silicone
filter adapter with the blue side facing the instrument.

VACUSIP should never be used without a filter.

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VACUSIP - Operating instructions V04 Installation

3.3.4 Connecting the silicone tubing

Place the bottle in the instrument and screw on the green lid with tube fittings (7).

Attach the silicone tubing coming from the filter to the fitting on the lid named “FILTER”.

Attach the longer silicone tubing to the fitting in the lid named “LIQ” (liquid) and the other

English

end to the VACUSIP hand operator (5

3.3.5 Replacing the battery

Unscrew the 5 screws on the bottom of the device with a
Phillips screwdriver nr. 1 and remove the bottom lid.

Unplug the old battery and replace it with an original
INTEGRA Biosciences rechargeable battery, see “7
Accessories and consumables” on page 14. Make sure that
it is inserted with the correct polarity (+/-).

Place back the battery in the battery compartment with the
wire facing to the inside of the instrument.

Close the bottom lid and fix it with the 5 screws.

).

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VACUSIP - Operating instructions V04 Operation

4 Operation

4.1 Vacuum activation and notes on use

Switch on VACUSIP to start the vacuum pump (the LED lights up green).

Attach a suitable adapter to the hand operator.

To aspirate press the soft buttons on both sides of the
VACUSIP hand operator.

Once the vacuum is reached, the pump stops until the vacuum falls below threshold.

VACUSIP switches off automatically after the pump has not been running for 10 minutes
or 40 minutes after initial switch-on. This feature can be temporarily deactivated by
pressing the power button for at least 4 seconds when switching on the instrument. The
power-off function is automatically restored after turning off VACUSIP.

Check the level of liquid in the bottle frequently. The liquid level should never reach the lid.

Prevent liquid or foam overflow from the collection bottle into the filter. In case the filter
gets accidentally wetted or soiled, exchange it immediately or otherwise the retention of
further liquid cannot be guaranteed. Note that the hydrophobic filter holds back aqueous
solutions, whereas retention of apolar liquids is limited.

ARNING

W

When working with dangerous substances, you mu st comply with the material
safety data sheet (MSDS) and the additionally applicable regulations of trade
associations, health authorities, supervisory offices, etc. In addition, when
working with hazardous volatile substances or biohazardous agents, either
place the VACUSIP inside a biosafety cabinet or connect the instrument's air
outlet (11) to a safety ventilation by means of a tube (ID=4 mm) and an
aspiration needle (Part. No. 155 502).

4.2 Code of indicator LED

English

LED code Meaning

Constantly green Unit is powered on and no errors occurred.

Blinking green Battery level low.

Blinking red once
per second

Blinking red 5 times
per second

Constantly red Battery error. Press the power button to continue using the

Blinking red and green Error in vacuum sensor calibration. Please contact your supplier.

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Leakage detection. Pump turns off if end vacuum has not been
reached after 5 minutes.

Vacuum generation is too fast (~100 mbar/s). Indicates a
clogged part.

mains power supply.

VACUSIP – Operating instructions V04 Operation

English

(model 159000) is discharged. Check indicator LED light.

VACUSIP automatically switches off if it is not used or 40

minutes after initial switch-on.

tubing, filter and hand operator for leaks. If the maximum

vacuum is no reached for 5 min, the pump is turned off.

Empty bottle and exchange filter.

Unit automatically switched off. Switch on the unit (LED lights up green).

4.3 Troubleshooting

Problem Probable cause Remedy

Device is not running. No electrical power. Check if mains adapter cable is plugged in or if battery

10 INTEGRA Biosciences AG

Aspiration speed is low. Leak in the vacuum system. Close the bottle lid tightly and attach tubing firmly. Check

Liquid overflowed and blocked the

Tubing is blocked. Clean or exchange the tubing.

Hand operator is clogged. Take apart the hand operator to clean it. Use an ultrasonic

filter.

bath to clean it of debris.

The LED blinks red rapidly.

Check if filter is blocked and exchange it, if necessary.

Replace the hand operator.

Check if the filter adapter is blocked.

Clogging of a part close to the

pump.

VACUSIP turns off after

a few seconds or

minutes.

The pump automatically stops if the end vacuum is not

reached after 5 min. Check for leaks in the system and push

the power button again.

Leak in the vacuum system. The LED blinks red slowly.

VACUSIP - Operating instructions V04 Maintenance

5 Maintenance

5.1 Cleaning and servicing

WARNING

Always switch off the VACUSIP and disconnect it from the mains when carrying
out maintenance work.

Clean the VACUSIP housing with a lint-free cloth lightly soaked with mild soap solution in

distilled water or with a 70 % dilution of Isopropyl or Ethanol. Never use bleach (sodium
hypochlorite) or other solvents.

It is recommended to change the hydrophobic filter regularly, at least once a year.
Exchange the filter immediately if liquid has entered.

When working with solvents and other hazardous materials always rinse the hand
operator and tubing with water followed by 70% Ethanol.

5.2 Autoclaving and chemical sterilization

5.2.1 Autoclaving

All parts contacting the liquid can be autoclaved: 500 ml glass bottle, lid with tube fittings,
silicone tubing and hand operator. The hand operator should be taken apart for optimal
decontamination. To disassemble it, hold the silicone cover and pull out the inner part.
Recommendation regarding autoclaving: glass bottle for at least 20 min at 121 °C.

The bottle lid has to be always unscrewed and kept loosely on the bottle during
autoclaving.

Silicone may become brittle after extensive autoclaving. Replace the tubings if they are
damaged.

ARNING

W

The hand operator can be autoclaved sporadically for decontamination,
however it is not recommended to autoclave it regularly.
The filter and the instrument itself cannot be autoclaved.

5.2.2 Chemical sterilization

Two methods for chemical inactivation of biohazardous agents are commonly used:

• A suitable disinfectant is added to the full collection bottle and left to react for the
recommended time.

• The disinfectant is added to the empty bottle so that the aspirated liquid gets sterilized
as it accumulates in the bottle. Use disinfectants that are free of chlorine or other
corrosive agents, especially when corrosive vapors are generated and aspirated.

English

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VACUSIP - Operating instructions V04 Technical data

5.3 Equipment disposal

The VACUSIP device must not be disposed of with unsorted municipal waste.
Do not dispose of the device in a fire.

VACUSIP (159000) contains a Li-ion battery. Do not modify the battery in any

English

way. Dispose of the VACUSIP device and the battery separately in accordance
with the laws and regulations in your area governing disposal of devices
containing Li-ion batteries.

6 Technical data

6.1 Specifications

Vacuum range –250 mbar +/- 20%

Flow rate (air) Pump: 1.5 l/min +/- 30% at ambient pressure

Dimensions
(H x W x D)

Weight Base unit only: 500 g

Electricity supply Mains adapter input: 100–240 VAC, 50/60 Hz

Battery rechargeable, Li-ion, ANSI 1604 size

Degree of protection IP 31

Ambient conditions Altitude: up to 2000 m

125 x 120 x 162 mm (base unit)
Height: unit with bottle and hand operator mount 345 mm

Unit complete: 970 g (with glass bottle, tubing and hand operator)

Device input: 8–10 VDC, 6 W

Temperature: 5 – 40 °C
Humidity: max. rel. humidity 80% for temperatures up to 31 °C
decreasing linearly to 50% rel. humidity at 40 °C.

6.2 Chemical compatibility

The table below lists VACUSIP parts that come into contact with the aspirated liquid or its
aerosols and vapors, and rates the compatibility of these parts to a few of the chemicals
commonly used in laboratories. To determine the compatibility of a component to a
chemical not listed in the table, please consult one of the several tables available on the
internet. Note that the rating refers to soaking in the concentrated chemical; however,
more relevant here is the attenuated effect resulting from vapors and the diluted chemical.
It is recommended to test the compatibility of relevant components to a specific chemical
prior to extensive use.

INTEGRA Biosciences does not warrant that the information in the table is accurate or
complete and that any material is suitable for any purpose.

12 INTEGRA Biosciences AG

VACUSIP – Operating instructions V04 Technical data

Phenol PhOH

Dimethyl sulfoxide

DMSO

Nitric acid (20 %)

Hydrochloric acid

HCl (20 %)

Sodium acetate

(3M, pH 5.2)

Sodium hydroxide

NaOH

Isopropyl alcohol IPA

Ethanol EtOH

Acetic acid AcOH

English

JAVEL (e.g. NaClO)

INOX BCAABACAAA

Outer part Silicone ABAAACACAC

Adapters POM CCAAAABCAA

for continuous use.

determined by test.

Compatibility ratings:

A = Good, no or minor effects.

B = Fair, moderate effects, not recommended

Chemical compatibility chart

www.integra-biosciences.com 13

C = Critical, not recommended, suitability to be

Parts Materials

Hand operator Inner part PP AAAAAAAAAA

Lids PP AAAAAAAAAA

Gasket EPDM AAAAAAAAAA

Housing PVC ACBAAAAACB

Tubing Silicone ABAAACACAC

Bottle Single-use PP AAAAAAAAAA

Filter Membrane PTFE AAAAAAAAAA

Filter adapter Silicone ABAAACACAC

VACUSIP baseHousing PP AAAAAAAAAA

Pump housing POM C C A A A A B C A A

Pump membraneNBR ABBAAAACBC

VACUSIP - Operating instructions V04 Accessories and consumables

7 Accessories and consumables

Accessories Part No.

Adapter 1-channel stainless steel adapter 40 mm 155 502

English

Stainless steel rod For holding the hand operator mount 159 045

VACUSIP hand
operator mount

Mains adapter
(100 - 240 VAC,
50/60 Hz)

1-channel stainless steel adapter 150 mm 155 522

1-channel stainless steel adapter 280 mm 155 525

1-channel adapter for disposable tips/GripTips (pack of 5) 159 023

1-channel adapter with ejector for disposable tips 159 026

1-channel adapter with ejector for GripTips 159 027

4-channel stainless steel adapter 40 mm 155 524

8-channel stainless steel adapter 40 mm 155 503

8-channel adapter with ejector for disposable tips 159 024

8-channel adapter with ejector for GripTips 159 025

To park the VACUSIP hand operator 159 046

EU 156 631

US 156 630

JP 156 634

UK 156 632

AU 156 633

Consumables Part No.

Filter For protection of the pump, non sterile, 0.45 µm 153 016

Tubing Silicone, 3 mm ID, 2.5 m 171 023

Silicone, 3 mm ID, 25 m bulk roll 171 033

Tubing set VACU­SIP

Bottle INTEGRA borosilicate bottle with standard GL 45 blue

Hand operator
VAC USIP

Battery Li-ion 155 066

14 INTEGRA Biosciences AG

Consists of: VACUSIP Hand operator,
Silicone tubing 1.2 m, Silicone tubing 0.22 m
1 filter (non-sterile, 0.45 µm)

lid, 500 ml

Single-use PP bottle with GL 45 lid, 500 ml (pack of 10) 159 032

Green lid GL 45 with tube fittings (pack of 2) 159 035

To operate VACUSIP and attach adapters 159 020

159 040

159 031