Integra MEDIAJET Operating Instructions Manual
103950_V09
MEDIAJET Operating instructions
Declaration of Conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
comply with:
EU Regulations
Standards for EU
Standards for Canada and USA:
Zizers, November 4, 2016
Description Models
MEDIAJET 103005, 103 006
MEDIAJET vario 113000, 113001, 113002
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU
Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU
Restriction of Hazardous Substances 2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) 1907/2006
Safety requirements for electrical equipment for laboratory use EN 61010-1: 2010
Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes.
EN 61010-2-81: 2015
Electrical equipment for laboratory use - EMC requirements EN 61326-1: 2013
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements
UL 61010-1
Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including
interference that may cause undesired operation.
Part 15 of the
FCC Rules
Class A
Elmar Morscher
CEO
Thomas Neher
Quality Manager
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Table of Contents
Chapter 1 Introduction
1.1 Intended Use.................................................................................. 5
1.2 General safety instructions............................................................. 5
1.3 Symbols used................................................................................. 6
Chapter 2 Description of the device
2.1 Scope of delivery............................................................................8
2.2 Overview of MEDIAJET ...............................................................11
2.3 Operating panel............................................................................ 12
2.4 MEDIAJET in detail...................................................................... 12
2.5 MEDIAJET vario in detail ............................................................. 13
2.6 Reverse side and interfaces.........................................................14
Chapter 3 Installation
3.1 Unpacking ................................................................................... 15
3.2 Preconditions ...............................................................................15
3.3 Removing and fitting the conversion set of MEDIAJET vario....... 17
3.4 Assembly...................................................................................... 19
3.5 Biplate option ............................................................................... 21
3.6 Turbo mode option....................................................................... 22
3.7 TUBEFILLER option.....................................................................23
Chapter 4 Operation
4.1 Overview MAIN MENU functions ................................................. 25
4.2 Adapt system parameter......................................
........................ 26
4.3 Loading Petri dishes.....................................................................28
4.4 Adapting a dish filling program..................................................... 30
4.5 Run dish filling program ............................................................... 34
4.6 Removing filled Petri dishes......................................................... 38
4.7 Process documentation................................................................ 39
4.8 Dosing function ............................................................................ 41
4.9 Mixing additives into the agar.......................................................42
4.10 TUBEFILLER option...................................................................44
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Chapter 5 Maintenance
5.1 Cleaning .......................................................................................48
5.2 UV Lamp exchange......................................................................50
5.3 Leak test.......................................................................................51
5.4 Servicing.......................................................................................52
5.5 Fault notifications .........................................................................52
5.6 Disposal........................................................................................53
Chapter 6 Technical data
6.1 Specification of the device............................................................54
6.2 Petri dish specifications................................................................55
6.3 Configuration specifications .........................................................56
6.4 Test tube compatibility chart.........................................................57
Chapter 7 Optional functions and required accessories
7.1 General accessories.....................................................................58
7.2 Accessories for different Petri dish sizes......................................58
7.3 Consumables ...............................................................................60
7.4 Imprinting dishes ..........................................................................60
7.5 Turbo mode option .......................................................................61
7.6 Accessories for dosing function....................................................62
7.7 Driving a second pump.................................................................63
7.8 TUBEFILLER option.....................................................................64
Imprint ..........................................................................................................................66
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1 Introduction
This operating instructions contain all information required for installation,
operation and regular maintenance of MEDIAJET. It summarizes all important
technical data and provides an overview of available accessories and options.
Purpose This chapter informs you regarding the general safety instructions and the
symbols used in the operating instruction manual.
1.1 Intended Use
This is a general-purpose laboratory instrument. Any use of this instrument in a
medical or IVD setting is the sole responsibility of the user.
The MEDIAJET is used in laboratories to fill Petri dishes of various sizes, Petri
dishes with two compartments or test tubes of various diameters and length.
1.2 General safety instructions
The MEDIAJET conforms to the state of technology and the recognized safety
rules, and is operationally safe. Operate the MEDIAJET only when in a perfect
condition and observing this operating instruction manual.
Residual dangers can emanate from the device if it is used or operated
incorrectly by untrained personnel.
All persons entrusted with operation of the MEDIAJET must have read and
understood this operating instruction manual and, in particular, the safety
instructions, or must have been instructed by their superior such that they are
able to operate the device without danger.
Irrespective of the safety instructions set out here, applicable provisions and
regulations must be observed in addition; for example, GLP, GMP, FDA, of the
employer's liability insurance associations, of the health authorities and of the
trading standards authorities.
Please observe the danger references on the device.
Do not carry out any conversion work or alterations on the device.
Please visit our website www.integra-biosciences.com
on a regular basis for up
to date information regarding REACH classified chemicals contained in our
products.
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1.3 Symbols used
This operating instruction manual makes specific reference to residual dangers
using the symbols shown.
1.3.1 Safety alert symbol
1.3.2 Severity levels of hazards in these operating instructions
The signal word in the top panel designates the hazard severity level.
1.3.3 Work reference
SAFETY SIGN
This is the general warning sign. It is used to alert the user to potential personal
injury hazards. In addition, hazards for machinery, materials and the
environment are possible. All safety messages that follow this sign shall be
obeyed to avoid possible harm.
WARNING
Indicates a hazard with a medium level of risk, which, if not avoided, could
result in death or serious injury.
CAUTION
Indicates a hazard with a low level of risk, which, if not avoided, could result in
minor or moderate injury.
NOTICE
Means that material damage or data loss in a micro-processor control could
occur, if the corresponding precautionary measures are not taken.
ASSISTANCE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
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1.3.4 Warning labels on the device
DANGER UV LAMP
Do not directly stare into the UV lamp.
MOVING PARTS
Keep hands out of area of moving parts.
ROTATING COG WHEELS
Keep hands out of cog wheels, risk to squash your fingers.
BIOHAZARD
The instrument can be potentially biohazardous due to the use of
biozazardous substances by the operator.
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2 Description of the device
MEDIAJET is a laboratory device designed for the filling of Petri dishes with agar
nutrient media. MEDIAJET is available in two different device versions. The
standard version is limited to the filling of 90/100 mm (nominal size) diameter
Petri dishes. The MEDIAJET vario version is convertible and provides the
possibility to fill Petri dishes of 35 mm, 60 mm and 90/100 mm diameter (nominal
size). The MEDIAJET vario with biplate option (Part No. 113 002) allows the
filling of Petri dishes with two compartments.
Purpose This chapter describes the most important components of the MEDIAJET and
introduces you to the specific terminology.
2.1 Scope of delivery
This section gives an overview of all required parts of an operable MEDIAJET
unit. Please also refer to packing list included in the shipment, as the total scope
of delivery is dependent on the respective device version and possible additional
accessories.
Basic device
(Part No. 103 005, 103 006, 113 000, 113 001
or 113 002)
Safety cover
(Part No.103 422) or
Biplate safety cover with reflector for alignment
sensor
(Part No. 113 801)
Rotor, different sizes possible
(Part No. 103 271, 103 272, 113 460, 113 271,
113 276 or 113 272) or
rotor for biplate option
(Part No. 113 806)
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Base plate for filling nozzle holder for Petri
dishes with a height above 21 mm
(Part No. 103 212) or
Base plate for filling nozzle holder for Petri
dishes with a height above 21 mm
(MEDIAJET vario with biplate option only)
(Part No. 113 821)
Tubing set, including 1.5 m silicone tubing and
filling nozzle (two sets for MEDIAJET vario with
biplate option)
(Part No. 103 030 or 113 030)
Spirit level
(Part No. 103 095)
Line cord
Conversion set installed, applies to MEDIAJET
vario only,
(Part No. 113 051, 113 055, 113 052, 113 056
or 113 053)
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2.1.1 TUBEFILLER option
The TUBEFILLER option (Part No. 103 010) converts the MEDIAJET into a test
tube filler.
10 pack O-rings (MEDIAJET vario with biplate
option only)
(Part No. 113 822)
Carousel, in a separate box
(Part No. 103 020, 103 021, 113 022, 113 020
or 113 021)
Support plate, can accommodate 3 test tube
racks.
Filling arm, consisting of carrier piece (1), rod
(2) and radial arm (3).
Silicone tubing set, consisting of silicone tubing
3.0 mm ID, 2.5 m length, stainless steel
aspirating and dispensing tube, each of 10 cm
length.
3
2
1
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2.1.2 Turbo mode option
The turbo mode option accelerates the filling process by using a second pump
head installed. This option requires the turbo mode kit (Part No. 103 036)
including:
2.2 Overview of MEDIAJET
Extension pump head (Part No. 171 090)
Tubing set for Turbo mode (Part No. 103 035),
inner diameter 6 mm, length 2.0 m.
1 Carousel
A standard carousel can accommodate
360 Petri dishes divided up into nine
stacks of 40 dishes. One position in the
carousel must be kept free at all times to
enable perfect working of the device.
2 Safety cover
The safety cover from Plexiglas reduces
the penetration of germs from the ambient
air into the filling chamber and protects the
user against UV radiation.
3 Peristaltic pump
4 Operating panel
(see 2.3)
5 Main switch
1
5
4
3
2
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2.3 Operating panel
2.4 MEDIAJET in detail
12 Cooling plate (factory option)
This cooling plate, which is available as a factory option built into the base
unit, accelerates the solidification of the agar medium in the Petri dish.
13 Rotor
The rotor separates the dish lid from the bottom, and transports the dishes
from the feeder position via the filling position to the stacker position.
14 Filling nozzle
The filling nozzle is part of the tubing set and establishes contact between
the MEDIAJET and the media preparator (MEDIACLAVE). It is positioned
correctly in the device by the filling nozzle holder.
6 Graphic user surface
7 Arrow keys
8 Function keys for operation of the
MEDIAJET
6
7
8
9 UV lamp
A UV lamp for decontamination of the
filling chamber is built in and switches off
automatically when the safety cover is
open.
10 Feeder position
At this position, the Petri dishes are
transferred from the carousel into the rotor
by the feeder piston.
11 Stacker position
At this position, the stacker piston transfers
the filled Petri dishes from the rotor back
into the carousel.
9
14 13 12
1110
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2.5 MEDIAJET vario in detail
MEDIAJET vario can be adapted to different diameters of Petri dishes, using a
conversion set.
The following parts of the conversion set can be exchanged:
The carousel, rotor and filling nozzle must also fit to the Petri dish size.
Description of MEDIAJET vario with biplate option:
The biplate option is used for filling Petri dishes with two compartments.
The following parts are specific for the biplate option.
The different parts of the conversion set are
already installed on the device.
15 Adaptation insert for Petri dishes of the
desired diameter.
16 Head plates for feeder and stacker piston
and flanges, laid underneath.
17 Adaptation insert with petri dish
guide pin.
18 Flanges for feeder and stacker.
19 Head plates for feeder a n d stacker
piston.
20 Extension gripper lever (applies
to conversion set for
35 and 60
mm Petri dishes only).
21 Set of spare screws and O-rings.
22 Alignment sensor at filling
position.
23 Drive wheel for rotating the Petri
dishes.
24 Filling nozzle holder with
integrated drive wheel.
25 Double pump head.
The Safety cover has a reflector,
needed for the alignment sensor.
15
16
17 18 19
20 21
22 23
24
25
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2.6 Reverse side and interfaces
Interface Function
26 FOOT-
SWITCH
Connection for the foot-switch
27 RS232(1) Service connection
28 RS232(2) Connection to an external protocol printer or to
MEDIACLAVE
29 INKJET Connection for an inkjet printer
30 PUMP Connection for controlling an additional peristaltic pump
31 AUX Potential-free connection for an external alarm system
32 SPARE Without function
26 27 28 29 30 31 32
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3 Installation
Purpose This chapter shows the unpacking and installation of MEDIAJET.
3.1 Unpacking
3.2 Preconditions
3.2.1 Operating environment
Generally inadmissible are locations with which there is a risk of spray water or
of contact with chemicals. The correct location satisfies the form of protection IP
21 and has a stable, horizontal base.
In the event of failure to observe the ambient conditions for the location, the
capacity to function and the safety cannot be guaranteed.
ASSISTANCE
Check the scope of delivery for any transport damage and notify your local
INTEGRA Biosciences AG representative (see last page of the operating
instructions).
Unpack the MEDIAJET and check the
scope of delivery (see “2.1 Scope of
delivery” on page 8).
Ambient temperature for operation 15-35°C
Maximum relative humidity Non-condensing, 30-80 % for up to
35 °C
Voltage fluctuations of the mains
supply
+/-10 % from the nominal value
Degree of contamination 2 as per IEC 60664-1
Ingress protection according to IEC 60529:
IP20 - Protection against objects >12.5 mm
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3.2.2 Setting up
CAUTION
The basic device must only be set up on a cleaned, dry and perfectly
horizontal surface, capable of supporting a minimum weight of 80 kg.
ASSISTANCE
Leave sufficient space behind the device to allow easy connection of the
mains cable.
At least two persons are required to lift the
instrument. Lift the instrument holding it
steady and firmly at the base of both sides
beside the feet.
Set up the basic device on the bench. In
order to obtain a perfectly horizontal
alignment of the unit, use the included spirit
level and adjust the height of the equipment
feet with a 13 mm flat spanner.
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3.3 Removing and fitting the conversion set of MEDIAJET vario
The MEDIAJET vario can handle different Petri dish sizes. To adapt the device
to the desired Petri dishes, the appropriate conversion set must be mounted.
3.3.1 Overview
3.3.2 Dismounting in detail
Removing the adaption insert
Conversion from
90 mm
to
35 or 60 mm Petri dish size
Conversion from
35 or 60 mm
to
90 mm Petri dish size
Removing the conversion set:
• Remove the adaption insert
• Unscrew the piston head plates
• Remove the flanges
Removing the conversion set:
• Remove the adaption insert
• Remove the extension gripper
lever
• Take off the piston head plates
• Remove the flanges
Inserting the conversion set for
35 or 60 mm Petri dishes:
• Insert the corresponding flanges
• Insert the corresponding head
plates tightly
• Mount the extension gripper lever
• Mount the adaption insert
Inserting the conversion set for
90 mm Petri dishes:
• Insert the corresponding flanges
• Mount the corresponding head
plates
• Mount the adaption insert
NOTICE
Make sure that the head plates of the
35 or 60 mm conversion set are tightly
inserted. Otherwise the Petri dishes may not be handled properly.
To exchange the conversion set, switch to the
CLEANING mode of the MAIN MENU.
Remove safety cover, carousel and rotor.
Unscrew the three countersink screws
M4x8 on the adaptation insert.
Remove the adaptation insert.
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Removing or mounting the extension gripper lever
Removing the piston head plates
Removing the flanges
Applies to conversion set for
35 and 60 mm
Petri dishes only:
Unscrew the two sink screws M3x6 of the
right and left extension gripper lever for
small petri dishes.
Keep the STACKER or FEEDER keys of
the operating panel pressed and
consecutively move both pistons up to the
high of the carousel plate using the
ARROW KEYS.
If present, unscrew the four piston head
screws M3x6 of the head plates and remove
the plates.
Unscrew the six piston head screws M3x6
of the flanges.
To lift the flanges move feeder and stacker
all to the top.
Remove the two flanges.
NOTICE
For an error free performance of MEDIAJET vario with the desired Petri dish
size, make sure that the corresponding conversion set, including the
adaptation insert, extension gripper lever, head plates and flanges is
completely installed and that the correct rotor and carousel are mounted.
After every exchange of the conversion set perform the dish sensor teach-in
procedure, see “4.2.2
Dish sensor teach-in (teach-in sensors only)” on
page 27.
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3.4 Assembly
3.4.1 Rotor
3.4.2 Safety cover
Slide the rotor under the black plate.
Turn the rotor until the anchor bolt locks into
position.
Hang the safety cover into the two hinges
on the right hand side of the device.
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3.4.3 Carousel
3.4.4 Electricity supply
The device is now fully assembled.
Now switch on the device at the main switch.
Wait until the device has completed the automatic software initialization.
When the main menu with INTEGRA logo appears on the screen, the device
has been installed and is ready for use.
NOTICE
Always lift the carousel on the thick central rods, never on the outer, flexible
rods.
Keep the carousel vertical and place it on
the bolt in the center of the black carousel
base plate.
Turn the carousel until the anchor bolt locks
into position.
Plug the electricity cable into the socket on
the rear of the device.
Subsequently connect it to the electricity
mains.
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