Declaration of conformity | Konformitätserklärung |
Déclaration de conformité | Declaración de conformidad |
Dichiarazione di conformità
INTEGRA Biosciences – 2 Wentworth Drive, Hudson, New Hampshire 03051
declares on its own responsibility that the product | erklärt in alleiniger Verantwortung,
dass das Produkt | déclare sous sa responsabilité exclusive, que le produit |
declara bajo su propia responsabilidad que el producto | dichiara sotto la propria
responsabilità che il prodotto
VIAFLO Electronic
Pipettes
in accordance with EC directives | gemäss der EU-Richtlinien | est conforme au terme
directives CE | de acuerdo con las directivas CE | in conformità alle direttive CE
2006/95/ECLow voltage equipment
2004/108/ECElectromagnetic compatibility
2011/65/ECRestriction of Hazardous Substances
2002/96/ECWaste Electrical and Electronic Equipment
is in compliance with the following normative documents: | mit den folgenden normativen
Dokumenten übereinstimmt: | aux documents normatifs ci-après: | cumple las
documentos normativos: | soddisfa le normative seguenti:
EN 61010-1Safety requirements for electrical equipment for measurement,
EN 61326-1 Electrical equipment for measurement, control and laboratory
Standards for Canada and USA
CAN/CSA-C22.2
No. 61010-1
UL Std. No. 61010-1Safety requirements for electrical equipment for measurement,
All rights to this documentation are reserved. In particular the rights of reproduction,
processing, translation and the form of presentation lie with INTEGRA Biosciences AG.
Neither the complete documentation nor parts thereof may be reproduced in any way, or
stored and processed using electronic media or distributed in any other way without the
written consent of INTEGRA Biosciences AG.
This operating instruction manual has part number 900-00002-00, the version is V05. It
applies to firmware version 2.44 or higher until a newer revision is released.
VIAFLO, VOYAGER, VIALINK, and GripTip are trademarks of INTEGRA Biosciences,
Hudson, NH.
Manufacturer
INTEGRA Biosciences AG
CH-7205 Zizers, Switzerland
T +41 81 286 95 30
F +41 81 286 95 33
info@integra-biosciences.com
www.integra-biosciences.com
INTEGRA Biosciences Corp.
Hudson, NH 03051, USA
T +1 603 578 5800
F +1 603 577 5529
Customer service
Please contact your local INTEGRA Biosciences representative.
To find out name and address of your local representative go to
www.integra-biosciences.com
Further information and operating instructions in other languages are available on
www.integra-biosciences.com
These operating instructions contain all the information required for installation, operation
and maintenance of the VIAFLO Electronic Pipettes. This chapter informs about the
symbols used in these operating instructions, the intended use of the VIAFLO Electronic
Pipettes and the general safety instructions.
1.1Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
ARNING
W
This safety symbol warns against hazards that could result in injury. Italso
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
CAUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
OTE
N
This symbol identifies important notes regardi ng the correct operation of the
device and labour-saving features.
1.2Intended use
VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven
pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5 5000 µl using GripTip pipette tips.
VIAFLO Electronic Pipettes comply to the recognized safety regulations and is safe to
operate. VIAFLO Electronic Pipettes can only be operated when in perfect condition and
while observing these operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the VIAFLO Electronic Pipettes must have
read and understood these operating instructions, and particularly, the safety notes, or
must have been instructed by supervisors so that safe operation of the device is
guaranteed.
Use only an INTEGRA battery.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Do not open or modify the VIAFLO Electronic Pipettes in any way. Repairs may only be
performed by INTEGRA Biosciences AG or by an authorised after-sales service member.
Parts may be replaced with original INTEGRA Biosciences parts only.
ARNING
W
Do not use the VIAFLO Electronic Pipettes near flammable material or in
explosive areas. Also, do not pipette highly flammable liquids such as
acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
Do not immerse the VIAFLO Electronic Pipettes in liquid. The fluid can
damage internal parts. Avoid pipetting of liquids whose vapors could attack
the materials PA (polyamide), POM (polyoxymethylene), FPM (fluor -rubber),
NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive vapors could also
damage metallic parts inside the device.
OTE
N
Prolonged exposure of the VIAFLO Electronic Pipettes to UV-light can cause
discolouration and/or yellowing of the control unit. However, this will not
affect the performance of the device in any way.
8INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05Description of the device
• Bag of spare O-rings (300 and 1250 µl versions only)
• Accuracy and precision certificate
AUTION
C
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is damaged,
instead contact your local dealer.
2.2Pipette configurations
VIAFLO single and multichannel pipettes couple an easy-to-use touch wheel controller
with intuitive programming. They are microprocessor controlled and stepper motor driven.
In addition, the VIAFLO VOYAGER adjustable tip spacing pipettes provides you with the
ability to vary tip spacing.
www.integra-biosciences.com9
VIAFLO Pipettes – Operating instructions V05Description of the device
1
2
5
6
7
8
3
4
9
11
12
10
2.3Overview of the VIAFLO Electronic Pipettes
2.3.1VIAFLO pipette parts
1 Display
2 Back button, to navigate backward
3 Touch wheel, spin to scroll and move the cursor
4 OK button, to make a selection
5 Left and right arrow buttons, for selections
6 Purge button, to empty tips
7 Run key, to start operations
8 Tip ejector
9 Finger hook, facilitates easy operation
10 Volume indicator label, color matches GripTip rack
insert.
11 Ejector sleeve
12 Tri-lobe tip interface
10INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05Description of the device
13
14
15
16
RUN
Current mode
Active step
OK
2.3.2Back view
13 Reset button
14 Power connector
15 Charging stand interface
16 Battery
2.3.3Display
The Display shows all pipetting options.
Battery life indicator
Instruction
Vol umes
Options
www.integra-biosciences.com11
VIAFLO Pipettes – Operating instructions V05Description of the device
2.3.4Touch wheel
The Touch wheel is fully operational with only one hand. Rotational finger movements
translate into up and down cursor movement on the display. The Touch wheel is fully
functional with the use of latex gloves.
Move finger on the Touch wheel to choose (and
highlight) an option on the display. Press OK (4
make the selection.
When a setting dial is displayed, spin the Touch wheel
to change the value and press OK.
2.3.5Left and right arrow buttons
At times, you will see and on a display screen. These buttons
are used to select options.
Press to select the option indicated with the left arrow (HELP, in
the example beside). Press to select the option indicated with the
right arrow (RUN, in the example).
For Voyager models, these buttons are also used for tip spacing adjustments.
2.3.6Purge button
During pipetting, you can interrupt the current pipetting protocol and purge all remaining
liquid currently in the GripTips. To do so, press Purge (6
).
) to
The pipette will display a prompt:
To proceed, press and release the Run key (7
of the dispense, the first step in the current program will be
displayed.
2.3.7Run key
Press and release the Run key to initiate aspiration, dispense, mix, purge, and special
pipetting operations. This button is centrally located for left- or right-handed actuation.
During dispense, you can press and hold the Run key to perform a two-step blowout, see
“4.3.2 Blowout modes” on page 21.
12INTEGRA Biosciences AG
). Upon completion
VIAFLO Pipettes – Operating instructions V05Description of the device
2.3.8Tip ejector
The tip ejector easily ejects tips from the tip cones.
The serial number can be found beneath the tip ejector. Press and
hold the tip ejector in the down position to see the 7-digit serial
number.
2.3.9Reset button
The reset button (13
RAM in the pipette. The programs stored in memory are maintained. Once pressed, the
VIAFLO startup screen will be displayed.
Press any key to continue and allow the instrument to initialize and home the pipette. The
routine ends by displaying the Main Menu.
) is located on the back of the VIAFLO pipettes. It is used to reset the
The VIAFLO Electronic Pipettes have been designed for use in a laboratory. They shall be
operated in a dry and dust-free location with a temperature of 5–40 °C and a maximal
(non-condensing) relative humidity of 80 %.
3.2Charging the battery
All VIAFLO hand-held pipettes share the same rechargeable, long-life, lithium-ion battery
that has a capacity of 1050 mAmp/hours. The battery should be charged for at least one
hour before pipetting. A full charge takes 2.5 hours and will provide approximately 3500
pipetting cycles.
A battery indicator is provided on the pipette display (2.3.3
• Blinking red icon: Low battery. A recharging message will appear.
• Blinking grey icon: Pipette is recharging.
• Blinking green icon: Pipette is recharging and battery is 90% charged.
• Solid green icon: Battery is fully charged and the pipette is still connected to the mains
adapter.
• Three static grey bars: Battery is fully charged.
AUTION
C
Use only the approved INTEGRA battery, power supply or charging stand.
Use of an incompatible power transformer can damage the pipette.
Battery charging can take place in two ways, using a pipette charging stand or the
Universal Voltage Power Supply (UPS), that allows for pipetting while charging the battery,
see (“8.1
3.2.1Charging the battery on a stand
Use one of the different charging stands - single position with two- (#4210) or four prong
connector (#4211), or four position with two prong connector (#4215) - to charge the
battery.
Accessories” on page 64).
Place the pipette on the charging stand by fitting the
power receptacle (15, on the top back of the pipette)
over the prong connector on the top of the stand.
Plug the cable of the adequate power supply to the
socket at the base of the stand (see “8.1
on page 64).
) indicating different states:
Accessories”
AUTION
C
Always use the correct power supply for the charging stand.
The pipette will turn fully on when placed on the stand. When the dimming time is achieved
the startup screen is displayed on the pipette. When the turnoff time is reached, the pipette
turns off. For disconnecting simply lift the pipette up from the stand.
3.2.2Charging the battery with UPS
With the optional Universal Voltage Power Supply (UPS, #4200), you can use the pipette
while charging through the line cord.
Insert the UPS connector into the receptacle on the top back of the
pipette (14
It will take approximately 30 seconds of charging to store enough
voltage to begin running the motor.
The pipette will turn fully on when the line cord is connected. The current screen remains
displayed. If the pipette is idle while charging, the display may dim (see “4.1 Turn on/off
the device” on page 20), but it will continue to show the battery life indicator. When the
display shut off time is reached, the instrument will shut off.
). Plug the UPS into a wall outlet.
3.3Toolbox - adapt your VIAFLO Electronic Pipettes
The Toolbox provides options to adapt the device to appropriate applications, setting
personal preferences, calibration, computer connectivity and storing owner information.
Toolbox modeDescription
PreferencesCustomizes the system parameters.
Calibration & ServiceSets calibration and service history options.
CommunicationsEnables communication between your VIAFLO Electronic
Pipettes and a PC.
Owner InformationView your pipettor’s serial number and set a personal ID.
Help LanguageSets language specific help.
Write ProtectProtects programs or menu options from modification.
Preferences customizes your system parameters. Select a preference and press OK to
access.
Preference Description Range
SoundSimple tones indicate completion of operations and
errors. Select an option and press OK to change the
status of the beep tone between On and Off:
• Step complete: at the end of a program step
• Program complete: at program completion
• Purge key: when Purge is pressed.
• Error message: when an error message appears or
when illegal data entry is attempted.
• Spinner: When spinning the Touch w h e el.
• Last dispense: before the last dispense in Repeat
Dispense and Variable Dispense.
DisplayCustomizes your display. Press OK to select an option,
use the Touch wheel to display the desired value.
• Start up display: Select the startup display at one of
the following: VIAFLO Logo, None or Custom (up to
two, uploaded with VIALINK). Press to Save your
selection.
• Display dim time: The display will dim after a set number of minutes. A shorter dim time helps preserve battery life. Press OK to save your selection.
• Turn off time: By default, the pipette will turn off after 5
minutes of idle time. A shorter turn off time helps preserve battery life. You can change this setting. Press
OK to save your selection.
Main MenuSelect a function to be hidden from the main menu (Off)
and press OK, e.g. Tip Spacing, Pipet, Repeat Dispense,
Dilute, Pipet/Mix.On/Off
SpinnerAdjust your Touch wheel spin sensitivity.Low, Medium,
PipettingSelect an option and press OK.
• Purge key speed: Choose the desired purge speed
and press OK to save your selection.
• Delayed blowin: Choose a timed delay between the
blowout and the blowin (delay before the piston
homes) at the end of a dispense, if no two step blowout
is performed, see “4.3.2 Blowout modes” on page 21
These options enable you to set calibration features and review service history .
Calibration
& Services
CalibrationAllows for re-calibration of the VIAFLO Electronic
Calibration
Reminder
Time or
Cycles
Service History
Calibrate Tip
Spacing
After changing desired settings, press to Save.
Description Range
Pipettes to restore accuracy. The calibration factors for
Pipet and Repeat type are displayed.
To edit the calibration volumes, press .
• Target Volume: This is the volume you are interested
in using for the calibration.
• Actual Volume: This is the measured volume obtained
when dispensing the target volume.
• Factory Reset: Resets the correction factor back to
the original factory setting. Press to apply the factory
setting.
• Current Factor: Displays the factor currently in use.
Sets a calibration reminder based on a specified time
frame or number of pipetting cycles. When the calibration
reminder is displayed, press any key to confirm.
However, the reminder will reappear every time the
pipettor is turned on until you change the reminder time
or use the reset option.
• Timer: Press OK to turn the reminder timer On or Off.
• Remind in: Use the Touch wheel to set a reminder
interval for calibration (time in months or in thousands
of cycles).
• Reset: Resets the timer to the defined calibration interval. With this option highlighted, press OK and Save
to enable.
Displays notes of any service that took place on the
VIAFLO Electronic Pipettes listed newest entry first.
Starts calibration of tip spacing of VOYAGER pipettes.-
The VIAFLO Electronic Pipettes can be programmed from a PC via a Single Pipette
Programming Stand (#4211) or via wireless Bluetooth connection.
VIALINK is a pipette management software for the PC and is used to manage VIAFLO
Electronic Pipettes. It can be downloaded from the INTEGRA website in the product
section and is free of charge for all VIAFLO Electronic Pipettes customers. A detailed
description of the software, along with the operating instructions, can be found on the
website as well.
Communications
SerialTurn serial communication mode “On” to begin bi-
BluetoothEach pipette needs its own Blue Tooth chip. Blue Tooth
Press OK to toggle between On and Off.
3.3.4Owner Information
Owner
Information
VIAFLOThe first line is highlighted. Press to enter personal
In addition, information about your VIAFLO Electronic Pipettes, such as owner
identification, serial number (SN), pipette size, indication of single or multi channel, and
version number, are displayed automatically.
Description Range
On/Off
directional communication. To exit the communications
mode follow screen prompt or reset the VIAFLO pipette
). Serial communication further allows (in addition to
(13
the functions of Bluetooth):
• Firmware updates
• Bi-directional communication from PC to pipette.
On/Off
communication has an open field connectivity of about
100 meters. It can be used for:
• Create and manage custom pipetting programs
• Image downloads
• Service history tracking
Description Range
-
information for your VIAFLO Electronic Pipettes.
•Use the Touch wheel to highlight a character and
press OK. You can press to Delete the last character entered. After entering the desired text, press to
Save.
Select this option to protect programs and menu options from inadvertent modification.
The pipetting programs can still be used.
Write
protect
Keep the password in a safe place.
Description Range
You can choose the language in which all help screens
are displayed (English, French, German, Spanish,
simplified Chinese, Japanese). Scroll to the desired
language and press to Save.
Description Range
Select an option and press OK to switch protection On or
Off:
• Standard programs
• Custom programs
• Calibration
• Too lbo x
• VOYAGER
• Password protect: Protect the access to the write pro-
tect menu by selecting “On”.
• Edit password, if password protect is “On”. To enter a
password use the Touch wheel to highlight a charac-
ter and press OK. Press to save the password. The
password must be entered before you can access the
write protect menu.
-
On/Off
3.3.7Password key
This menu displays the encrypted password, if set. Should you lose your password,
contact INTEGRA Biosciences to retrieve your password using these numbers.
Remove hands from the Tri-lobe tip interface (12) at switch on and during
homing. Also do not touch the touch wheel, because it is calibrated during
the start up process.
The pipette flashes the startup screen and performs a full motor homing routine, ensuring
the motor is in the run position. “Home” is the base point for the pipette. Homing is the
process whereby the pipette motor moves the piston(s) to a sensor position. This position
ensures that no liquid remains in the tips. For the VOYAGER, homing also includes the tip
spacing motor. After homing, the tips move to the last position they occupied.
After homing the menu last used is displayed. Press the Back button (2
as necessary to return to the Main menu.
Turn off:
To turn off the pipette, press and hold the Back button (2
OTE
N
The VIAFLO Electronic Pipettes will dim and tu rn off automatically after a
preset duration of inactivity. This duration is 5 minutes, by default, and
configurable with the Toolbox (see “3.3.1 Preferences” on page 16).
) to turn on the pipette.
) as many times
) for 3 seconds.
4.2Attaching and removing GripTips
CAUTION
To ensure optimal performance of your VIAFLO Electronic Pipettes always
use GripTips which have been designated for the use with the VIAFLO
Electronic Pipettes, see “8.2
The unique tri-lobe design of the VIAFLO Electronic Pipettes reduces attachment and
ejection forces, ensures a perfect fitting to prevent the tips from falling off and to provide
a perfect sealing. On a multichannel pipette all tips sit on the same height.
Tri-lobes minimize contact surface and temporarily deforms the tip
shaft
Shoulder provides a positive stop to prevent over tightening and all
tips seal at the same height
O-ring provides forgiving and robust seal surface for the pipet tip
20INTEGRA Biosciences AG
Consumables” on page 64.
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