Integra PIPETBOY acu 2, 155015, PIPETGIRL, 155016, 155018 Operating Instructions Manual

PIPETBOY acu 2 Operating instructions

466079_V13

Declaration of conformity

INTEGRA Biosciences AG –

7205 Zizers, Switzerland

declares on its own responsibility that the devices

comply with:

EU Regulations

Standards for EU

Standards for Canada and USA

Zizers, November 4, 2016

Description Models

PIPETBOY acu 2 155000, 155015, 155016, 155017, 155 018, 155 019

PIPETGIRL 155021

EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW

Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU

Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU

Restriction of Hazardous Substances 2011/65/EU

Waste Electrical and Electronic Equipment 2012/19/EU

Battery Directive 2006/66/EC

Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)

1907/2006

Capacity Labelling of Portable Secondary Batteries 1103/2010

Ecodesign - Power supplies 278/2009

Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.

EN 61010-1: 2010

Electrical equipment for measurement, control and laboratory use ­EMC requirements.

EN 61326-1: 2013

Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.

CAN/CSA-C22.2

No. 61010-1

Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.

UL 61010-1

Operation is subject to the following two conditions: (1) this device may not
cause harmful interference, and (2) this device must accept any interfer­ence received, including interference that may cause undesired operation.

Part 15 of the

FCC Rules

Class A

Elmar Morscher
CEO

Thomas Neher
Quality Manager

PIPETBOY acu 2 - Operating instructions V13

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Table of Contents

1 Introduction ................................................................................................................ 4

2 Description of the device............................................................................................ 5

3 Installation .................................................................................................................. 6

4 Operation ................................................................................................................... 7

5 Maintenance............................................................................................................. 10

6 Technical Data ......................................................................................................... 11

7 Accessories.............................................................................................................. 12

Imprint

© 2018 INTEGRA Biosciences AG

This operating instruction manual applies to serial number 1 350 000 or higher of
PIPETBOY acu 2 / PIPETGIRL.

Manufacturer

Customer service

Please contact your local INTEGRA Biosciences representative, see
www.integra-biosciences.com

or contact [e-mail].

INTEGRA Biosciences AG

CH-7205 Zizers, Switzerland
T [phone]
F [phone]

INTEGRA Biosciences Corp.

Hudson, NH 03051, USA
T [phone]

F [phone]
[e-mail]
www.integra-biosciences.com

PIPETBOY acu 2 - Operating instructions V13 Introduction

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1 Introduction

1.1 Intended use

PIPETBOY acu 2 is a pipette controller designed for aspirating and dispensing aqueous
solutions with plastic or glass pipettes of 1 to 100 ml volumes. It is intended for
measurement, control and laboratory use. Any use of this instrument in a medical or IVD
setting is under the sole responsibility of the user.

PIPETGIRL is a special model of PIPETBOY acu 2 and provides the same functions as
PIPETBOY acu 2.

1.2 Safety notes

1) Do not use or charge PIPETBOY acu 2 in an atmosphere with danger of explosion.

Also, do not pipette highly flammable liquids such as acetone or ether.

2) When handling dangerous substances, comply with the material safety data sheet

(MSDS) and with all safety guidelines such as the use of protective clothing and safety
goggles. Never point a pipette in anyone’s direction.

3) Avoid pipetting of liquids whose vapours could attack the materials PA (polyamide),

POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrile-rubber), CR (chloroprene),
silicone. Corrosive vapours could also damage metallic parts inside the device.

4) Use an original INTEGRA Biosciences mains adapter only and protect it from moisture,

otherwise PIPETBOY acu 2 might be damaged.

5) Prolonged exposure of PIPETBOY acu 2 to UV-light can cause discolouration and/or

yellowing of the plastic housing. However, this will not affect the performance of the
device in any way.

6) Old Li-ion batteries may cause a safety risk. We recommend to replace the battery after

3 years of use. Also replace the battery if the charging intervals are unusually short or
if the charging takes much longer than usual (4 hours or more). These are indicators
that the battery has reached the end of its life-cycle.

7) Li-ion technology bears the risk of thermal runaway and cell rupture if the battery was

damaged. Do not expose the battery to heat (> 60°C) and avoid mechanical stress.
Batteries which were subject to deep discharges may develop internal short circuits,
leading to an increased self-discharge rate and heating during battery charging. This
may also result in thermal runaway and cell rupture.

8) To extend the battery life-cycle, it is recommended to charge the battery every 2

months if the pipette controller is not used regularly. If the pipette controller is not used
for more than 6 months, remove the battery from the instrument.

Regardless of the listed safety notes, additionally applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.

Please visit our website www.integra-biosciences.com

on a regular basis for up to date

information regarding REACH classified chemicals contained in our products.

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2 Description of the device

2.1 Scope of delivery

• PIPETBOY acu 2 / PIPETGIRL device

• 1 rechargeable Li-ion battery

• Mains adapter

• Wall mount

• Hydrophobic sterile filter 0.45 µm (spare)

• Quick Start Guide

2.2 Overview of PIPETBOY acu 2

1 Aspiration button

2 Dispensing button

3 Thumb wheel to set maximum speed

4 Battery charge indicator

5 Socket for mains adapter cable

6 Handle

7 Sterile module complete (=nose piece)

7 Sterile module complete (=nose piece)

7a O-ring

7b Housing of sterile module, upper part

7c Filter rubber

7d Hydrophobic filter

7e Pipette mount

7f Housing of sterile module, lower part

1

2

4

5

7

6

3

c

d

e

f

b

7a

PIPETBOY acu 2 - Operating instructions V13 Installation

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3 Installation

3.1 Charging the battery

A full charge takes 3.5 hours. Before the first use, PIPETBOY acu 2 should be charged
until the battery charge indicator (4) turns green, showing that the battery is full.

When the battery charge indicator (4

) starts flashing red, PIPETBOY acu 2 can be used
for around 100 pipetting cycles before shutting down. It should thus be recharged
immediately.

PIPETBOY acu 2 has an integrated protection: it will not overcharge even if it is connected
to power for indefinite time. To avoid unnecessary power consumption, it is recommended
to unplug the power supply when the charge indicator is green. PIPETBOY acu 2 can be
used while it is being charged.

The battery charge indicator provides various information:

3.2 Replacing the battery

3.3 Mounting of the wall mount

The wall mount serves to park PIPETBOY acu 2.

To mount the wall mount, remove the protective foil from the adhesive tape at the back of
the holder. Hold it with the sign <up> facing upwards and press it to the desired place.
Make sure that the surface onto which the wall mount is mounted is smooth, clean and
grease-free. Wait 24 hours before using the wall mount for the first time. Alternatively the
wall mount can be fixed with the included screws.

Battery charge indicator Battery status and information

Flashes red Battery is low. Charging is needed.
Is red and power supply is connected Battery is being charged.
Is green and power supply is connected Battery is fully charged.
Flashes alternately red and green Battery error. Check if the correct type of

battery with correct polarity (+/-) and/or
power supply are used.

1) Move the lid of the battery compartment upwards and
remove it (a).

2) Replace the old battery with an original
INTEGRA Biosciences rechargeable battery (see “7
Accessories” on page 12) and make sure that it is
inserted with the correct polarity (+/-).

3) Close the battery compartment with the lid (a).

a

PIPETBOY acu 2 - Operating instructions V13 Operation

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4 Operation

4.1 Inserting the pipette

WARNING

Do not insert pipettes with force into PIPETBOY acu 2, because they can break
and cause injury, particularly thin pipettes made of glass.

4.2 Pipetting

Press the aspiration button (1) to fill the pipette and the dispensing button (2) to empty it.

The aspiration and dispensing speed can be controlled in two manners:

• Fine speed adjustment by varying the finger pressure on the buttons (1

, 2).

• Step-less presetting of the maximum pump speed by turning the thumb wheel (3

) to
optimally match the pipette volume (turning to the left = slower pump speed, for small
pipettes; to the right = faster, for large pipettes).

To empty the pipette by gravity force, press the dispensing button only slightly in order to
avoid reaching the trigger point where the pump starts running. Gravity dispensing is used
for “to deliver” (TD) pipettes that are not

of the “blow-out” type (blow-out pipettes have two

thin rings or a frosted band around the neck).

PIPETBOY acu 2 is featured with a “TURBO” mode. Plug the mains adapter cable into the
pipette controller and turn the thumb wheel completely to the right for maximal speed.

The silicone pipette mount (7e

) has a special conical
channel to guarantee a firm and leak-proof grip of the
pipette independently of its diameter.

Disassemble the nose piece (see “5.1

Cleaning and
servicing” on page 10) and orient the pipette mount:
a) with the large opening facing down for pipettes > 2 ml

(factory setting), or

b) with the small opening facing down for pipettes < 2 ml.

a) b)

PIPETBOY acu 2 – Operating instructions V13 Operation

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4.3 Troubleshooting

Problem Probable cause Remedy

Pipette drips

(leak in the system).

Pipette is damaged or not fully inserted in

the nose piece (7

).

Reinsert a new pipette and push it all the way into

the nose piece. Make sure that the pipette mount

orientation is correct (see section 4.1).

The inside of the pipette mount (7e

) is

damaged resulting in insufficient sealing of

the pipette neck.

Replace the pipette mount (#151 020).

The filter rubber (7c

) or the filter (7d) in the

nose piece are damaged or missing causing

a leak.

Replace the filter rubber (#153 225) and/or the

filter (Part No. see “7 Accessories” on page 12).

Reduced aspiration

efficiency or no liquid

aspiration.

The filter (7d

) is wet or dirty. Replace filter.

The nose piece (7) is not tight. Tighten the nose piece, or replace defective parts.

The battery is discharged (battery charge

indicator flashes red)

Charge the battery.

The battery is missing. Insert the battery or connect the instrument to the

mains adapter.

The battery is defective. Replace the battery.

The battery is wrongly inserted. Insert correctly, note polarity (+) and (-).

Reduced operating time

with fully charged battery.

The battery is worn. Replace the battery.

Wrong battery type is inserted. Use only original Li-ion battery (#155 066).

Extremely long charging

time of battery.

Wrong mains adapter is used. Use only original mains adapter (see “7

Accessories” on page 12).

Extremely short charging

and operating time.

Wrong battery type is inserted. Use only original Li-ion battery (#155 066).

PIPETBOY acu 2 – Operating instructions V13 Operation

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Battery is not charging. The battery is wrongly inserted. Insert correctly, note polarity (+) and (-).

Wrong battery type is inserted. Use only original Li-ion battery (#155 066).

Wrong mains adapter is used. Use only original mains adapter (see section 7

).

Battery operation not

possible.

The battery is wrongly inserted. Insert correctly, note polarity (+) and (-).

Wrong battery type is inserted. Use only original Li-ion battery (#155 066).

The battery is missing. Insert the battery.

Problem Probable cause Remedy

PIPETBOY acu 2 - Operating instructions V13 Maintenance

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5 Maintenance

After maintenance work, perform a leak test to ascertain correct functioning of
PIPETBOY acu 2: liquid should not leak out of a filled pipette before the dispensing button
is pressed.

5.1 Cleaning and servicing

PIPETBOY acu 2 can be cleaned with a cloth moistened with soapy water or with a 70 %
ethanol.

Disassembly of the nose piece:

It is recommended to change the hydrophobic filter (7d

) every three months. Should the
filter get wetted or soiled, it has to be changed immediately. The filter must be oriented
with the blue (0.45 µl) / red (0.2 µl) side facing upwards towards PIPETBOY acu 2.

5.2 Decontamination

The nose piece housing (7b, 7f), the pipette mount (7e) and the filter rubber (7c) can be
autoclaved at 121 °C, 1 bar overpressure for 20 minutes. Silicone may become brittle after
extensive autoclaving. Replace the pipette mount and filter rubber if they are damaged.

If the housing of the PIPETBOY acu 2 have been in contact with biohazardous material,
it must be decontaminated in accordance to good laboratory practice. Do not spray directly
on the instrument but use a lint-free cloth, lightly soaked with a disinfectant and wipe dry
directly after decontamination. Never use acetone or other solvents! Follow the
instructions provided by the disinfectant manufacturer.

5.3 Equipment disposal

PIPETBOY acu 2 device must not be disposed of with unsorted municipal
waste. Do not dispose of the device in a fire.

PIPETBOY acu 2 contains a Li-ion battery. Do not modify the battery in any
way. Dispose of the PIPETBOY acu 2 device and the battery separately in
accordance with the laws and regulations in your area governing disposal of
devices containing Li-ion batteries.

Unscrew the nose piece (7

) from the handle by turning it
counter clockwise. Hold the upper part of nose piece (7b),
press the lower part (7f

) firmly against the upper part (7b)
and turn it counter clockwise (left). The lower part of nose
piece housing (7f

) will disengage after about 1/8th of a

turn.

Remove the pipette mount (7e

), the filter (7d) and the

filter rubber (7c

), if required.

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6 Technical Data

6.1 Specifications

6.2 Chemical compatibility

The table below lists PIPETBOY acu 2 parts that come into contact with the aspirated
liquid or its aerosols and vapors, and rates the compatibility of these parts to a few of the
chemicals commonly used in laboratories. To determine the compatibility of a component
to a chemical not listed in the table, please consult one of the several tables available on
the internet. Note that the rating refers to soaking in the concentrated chemical; however,
more relevant here is the attenuated effect resulting from vapors and the diluted chemical.
It is recommended to test the compatibility of relevant components to a specific chemical
prior to extensive use.

INTEGRA Biosciences does not warrant that the information in the table is accurate or
complete and that any material is suitable for any purpose.

Pipetting speed max. 13.5 ml/s (with a 50 ml serological pipette)
Battery Type: rechargeable, Li-ion, min. 500 mAh

Typical charging time: 3.5 hours
Charging cycles: 500–1000 (when charging as indicated)

Running time: at least 5500 cycles of aspiration and
dispensing of 25 ml.

Electricity supply Mains adapter input: 100–240 VAC, 50/60 Hz

Device Input: 16–19 VDC, 3.1 W

Materials Housing: PA

Nose piece housing: POM
Pipette mount: Silicone

Filter rubber: Silicone
Dimensions (H x W x D) 125 x 130 x 35 mm
Weight 195 g
Ambient conditions Operation: 5–40°C, max. 80% RH

Storage: -10–50°C, max. 95% RH

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12 INTEGRA Biosciences AG

Chemical compatibility chart

Compatibility ratings:
A = Good: no or minor effects.
B = Fair: moderate effects, not recommended for continuous use.
C = Critical: not recommended, suitability to be determined by test.

7 Accessories

Parts Materials

JAVEL (e.g. NaClO)

Acetic acid

Ethanol

Isopropyl alcohol

NaCl saturated

Sodium hydroxide (50%)

Sodium acetate

(3M, pH 5.2)

Hydrochloric acid (20%)

Chloroform

Acetone

Handle PA A C B A A A A C C A

Nose piece housing POM C C A A A A A B A A

Pipette mount,
Filter rubber, tubings

Silicone A B A A A A C A C C

Internal parts
(e. g. pump)

FPM AAAAAC C AAC

NBR ABBAAA A ACC

CR AAAAAA A ABA

Metal CCAABC A CAA

Accessories Part No.

Stand for
PIPETBOY

for a well organized work area, to park PIPETBOY acu 2 with
inserted pipette

155065

Wall mount for holding PIPETBOY acu 2 on the wall 155521
Mains adapter

(100-240 VAC,
50/60 Hz)

EU version 153210
US/JP version 153211
UK version 153214
AU version 153216

PIPETBOY acu 2 - Operating instructions V13 Accessories

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WARNING

Consumables Part No.

Sterile module
(nose piece)
complete (7

)

turquoise for PIPETBOY acu 2 classic and transparent;
with filter 0.45 µm, unsterile

155025

classic (grey) for PIPETBOY acu 2 green, red, blue, purple
and PIPETGIRL; with filter 0.45 µm, unsterile

155070

Sterile module
(nose piece)
housing (7b

,7f)

turquoise, for PIPETBOY acu 2 classic and transparent 155230
classic (grey), for PIPETBOY acu 2 green, blue, red, purple

and PIPETGIRL

155060

Filter 0.45 µm
(7d

)

sterile 153015
unsterile 153016

Filter 0.2 µm
(7d

)

sterile 156608
unsterile 156607

Pipette mount
(7e

)

silicone, for holding pipette in the sterile module (nose piece) 151020

Filter rubber (7c) silicone, for holding the filter in the sterile module (nose

piece)

153225

O-ring (7a

) 153235

Battery
compartment lid

classic (grey) 155203
green 155160
red 155161
blue 155162
transparent 155163
purple 155164
pink (PIPETGIRL) 155166

Battery Li-ion for PIPETBOY acu 2 / PIPETGIRL

(with serial number >=1350001)

155066

Only use the battery Li-ion for PIPETBOY acu 2
(serial number >=1350001; LED is white when inactive,
red or green when active)!
The NiMH battery for PIPETBOY acu is not compatible!

serial number