Implen OD600 DiluPhotometer User Manual
phone: + 49 (0) 89 726 3718 0 Fax. + 49 (0) 89 726 3718 54 Email: info@implen.de www.implen.de
The OD600 DiluPhotometer™
User Manual
Version 1.4
OD600 DiluPhotometer
TM
User Manual
Version 1.3 Page 2 / 14
Implen GmbH Schatzbogen 52 D-81829 Munich
Declaration of Conformity
This is to certify that the Implen OD600 DiluPhotometer™ conforms to the requirements of the
following directives:
2014/35/EU Low Voltage directive (LVD)
2014/30/EU Electromagnetic Compatibility directive (EMC)
2011/65/EU Restriction on the use of certain hazardous substances directive (RoHS)
2012/19/EU Waste electrical and electronic equipment directive (WEEE)
98/79/EC In Vitro medical devices directive4
2006/42/EC Machinery directive
Standards used to demonstrate conformity include:
EN 61010-1:2010 Safety requirements for electrical equipment for measurement, control and
laboratory use, General Requirements
EN 61010-2-101:2002 Safety requirements for electrical equipment for measurement, control and
laboratory use. Particular requirements for in vitro diagnostic (IVD) medical
equipment
EN 61326-1:2013 Electrical equipment for measurement, control and laboratory use –EMC
requirements (class B)
EN ISO 12100:2010 Safety of machinery-General principles for design, risk assessment and risk
reduction
Signed: Dated: June 20, 2016
Dr. Thomas Sahiri
Managing Director
Implen GmbH
OD600 DiluPhotometer
TM
User Manual
Version 1.3 Page 3 / 14
TABLE OF CONTENTS
1. ESSENTIAL SAFETY NOTES........................................................................................................................... 4
1.1 Unpacking, Positioning and Installation ........................................................................................... 4
2. INTRODUCTION ................................................................................................................................................ 5
2.1 Your Spectrophotometer .................................................................................................................... 5
2.2 Sample Handling Tips ......................................................................................................................... 5
2.3 Keypad and Display ............................................................................................................................ 6
3. DILUCELLTM ...................................................................................................................................................... 7
3.1 Description ........................................................................................................................................... 7
3.2 Operation Instructions ........................................................................................................................ 7
3.3 Specifications ...................................................................................................................................... 8
4. OD600TM - DILUCELLTM APPLICATION ........................................................................................................... 9
4.1 Bacterial Cell Culture Measurement .................................................................................................. 9
4.1.1 Description................................................................................................................................... 9
4.1.2 Operation Instructions ................................................................................................................. 9
5. OD600TM - STANDARD CUVETTE APPLICATION ........................................................................................ 11
5.1 Protein Quantification with Bradford Assay ................................................................................... 11
5.1.1 Description................................................................................................................................. 11
5.1.2 Operation Instructions ............................................................................................................... 11
6. TROUBLE SHOOTING NOTES ...................................................................................................................... 12
7. ACCESSORIES ............................................................................................................................................... 13
8. MAINTENANCE ............................................................................................................................................... 13
8.1 Cleaning and General Care of the Instrument ................................................................................ 13
8.2 De-contamination Procedure ........................................................................................................... 13
9. SPECIFICATION AND WARRANTY ............................................................................................................... 14
OD600 DiluPhotometer
TM
User Manual
Version 1.3 Page 4 / 14
1. ESSENTIAL SAFETY NOTES
1.1 Unpacking, Positioning and Installation
Check the contents of the pack against the packing list. If any shortages are discovered, please inform your
supplier immediately.
Inspect the instrument for any signs of damage caused in transit. If any damage is discovered, please inform
your supplier immediately.
Ensure your proposed installation site conforms to the environmental conditions for safe operation:
Indoor use only
Temperature 5°C to 35°C
Maximum relative humidity of 80 % up to 31°C decreasing linearly to 50 % at 40°C
The instrument is powered by the internal rechargeable battery or by mains electricity using the supplied power-
adapter. Using the instrument with the mains adapter will automatically recharge the battery.
The battery will last approx. 1 month when fully charged with normal use.
A full battery recharge will take approx. 12 hours (overnight).
Please read through this user manual prior to use.
Please contact your original supplier in the first instance if you experience technical or sample handling
difficulties.
If this equipment is used in a manner not specified or in environmental conditions not appropriate for safe operation, the
protection provided by the equipment may be impaired and instrument warranty withdrawn.
OD600 DiluPhotometer
TM
User Manual
Version 1.3 Page 5 / 14
2. INTRODUCTION
2.1 Your Spectrophotometer
Your OD600TM is a small, easy to use instrument that is dedicated to measuring samples at a wavelength of 600 nm. It is
suitable for measuring growth rates of all types of cell (e.g. E. coli and yeast). Combining OD600TM with DiluCellTM 10 or
DiluCellTM 20 facilitates measurements with low sample volumes of 200 µl and 100 µl, respectively and a virtual sample
dilution of either factor 10 or 20.
With a bandwidth of 40 nm the OD600TM enables also measurements nearby 600 nm like the protein quantification
assay according to Bradford (595 nm).
A 600 nm LED source in combination with a fibre optic is used to obtain the measurement. The instrument can be used
in incubation cabinets and under anaerobic conditions.
2.2 Sample Handling Tips
Note that the light beam is directed horizontal from the front of the instrument to the back; therefore please
ensure the cell is inserted in the correct alignment.
The optical height is 8.5 mm.
The cell holder supplied with the instrument accepts:
Pathlength
min. Volume
virtual Dilution
Adapter
DiluCellTM 10
1.0 mm
200 µl
1:10
No
DiluCellTM 20
0.5 mm
100 µl
1:20
No
Macro cuvette
(max fill volume 4.5 ml)
10 mm
1 ml
No
No
Semi-micro cuvette
(max fill volume 1.4 ml)
10 mm
0.5 ml
No
No
10 mm diameter tubes
n/a*
0.9 ml
No
Yes
12 mm diameter tubes
n/a*
1.1 ml
No
Yes
16 mm diameter tubes
n/a*
2.2 ml
No
No
*Depends on tube being used.
IMPORTANT WARNING
Always wear protective clothing when handling bacteria or other cells.
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