Implen NanoPhotometer NP80, NanoPhotometer N60, NanoPhotometer C40, NanoPhotometer N50 User Manual

NanoPhotometer® NP80/N60/N50/C40

User Manual

Version 3.1.1

Software Version 3.1.13220

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Declaration of conformity for the NanoPhotometer® (C40/N50/N60/NP80)

This is to certify that the Implen NanoPhotometer® conforms to the requirements of the following directives:

2014/35/EU Low Voltage Equipment Safety Directive 2014/30/EU Electromagnetic compatibility (EMC) directive IEC 60529 Protection class IP20 2011/65/EU Restrictions on the use of certain Hazardous Substances

in Electrical and Electronic Equipment (ROHS)

2012/19/EU EC Directive on Waste Electrical and Electronic Equipment (WEEE)

2003/108/EC & 2008/34/EC. By ensuring this product is disposed of correctly, you will help prevent potential negative consequences for the environment and human health, which could otherwise be caused by

inappropriate waste handling of this product. FCC 47 CFR Part15 §15.107 and §15.109 EN 301 489-1 V1.9.2 Radio and ancillary equipment for portable use (portable equipment); EUT

Operating frequency range: 2.4 – 2.4835 GHz EN 300 328 V1.8.1 Electromagnetic compatibility and Radio spectrum Matters (ERM);

Wideband transmission systems; Data transmission equipment operating

in the 2,4 GHz ISM band and using wide band modulation techniques;

Harmonized EN covering the essential requirements of article 3.2 of the

R&TTE Directive EN 301 489-17 V2.2.1 Electromagnetic compatibility and Radio Spectrum Matters (ERM) IEC 62133 and UN38.3 Battery certification and transport test Standards to which conformity is declared, where relevant, are as follows: IEC/EN 61010-1:2012 Safety requirements for electrical equipment for measurement, control,

and laboratory use. General requirements. EN61326-1:2013 Electromagnetic compatibility- generic emission standard electrical

equipment for measurement, control, and laboratory use. Signed:

Dr. Thomas Sahiri Managing Director

Implen GmbH

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Contents

1. NANOPHOTOMETER

NANOPHOTOMETER

NANOPHOTOMETER NANOPHOTOMETER NANOPHOTOMETER NANOPHOTOMETER

OVERVIEW .............................................................................................. 8

NP80 ................................................................................................................ 9

N60 ................................................................................................................. 10

N50 ................................................................................................................. 11

C40 ................................................................................................................. 12

AT A GLANCE ....................................................................... 8

INSTRUMENT REAR PANEL (NP80/N60/N50/C40) .............................................................................. 13

INSTRUMENT BOTTOM VIEW (NP80/N60/N50/C40) ............................................................................ 13

ACCESSORIES ....................................................................................................................... 14

STANDARD ACCESSORIES ................................................................................................................. 14

OPTIONAL ACCESSORIES .................................................................................................................. 14

CONNECTIVITY....................................................................................................................... 18

NANOPHOTOMETER

SPECIFICATIONS .................................................................................... 19

2. GETTING STARTED .............................................................................................. 21

SPECTROPHOTOMETER INSTALLATION .................................................................................... 21

SAFETY INFORMATION ...................................................................................................................... 21

UNPACKING AND POSITIONING .......................................................................................................... 22

SOFTWARE INSTALLATION ...................................................................................................... 23

NPOS OVERVIEW ................................ ................................................................ ............................. 23

REQUIREMENTS AND COMPATIBILITY ................................................................................................. 23

INSTALLING SOFTWARE ON COMPUTER ................................ .............................................................. 24

INSTALLING NANOPHOTOMETER

APP ON TABLET OR SMARTPHONE .................................................. 25

FIRST STEPS AND CONFIGURATION WIZARD ........................................................................................ 26

PRINTER INSTALLATION .................................................................................................................... 26

3. NANOPHOTOMETER

BASICS .............................................................................. 27

APPLICATIONS OVERVIEW ...................................................................................................... 27

ICONS ................................................................................................................................... 29

BUTTONS .............................................................................................................................. 30

SIDE TAB BAR .................................................................................................................................. 31

DATA PROCESSING DIALOGS ................................ ............................................................................. 33

BASIC OPERATION ................................................................ ................................ ................. 36

NANOVOLUME MEASUREMENT BASICS (N50/N60/NP80) ................................................................... 36

CUVETTE MEASUREMENT BASICS (C40/NP80) .................................................................................. 37

SUBMICROLITER CELL MEASUREMENTS BASICS (C40)....................................................................... 39

SAMPLE HANDLING TIPS ................................................................................................................... 40

DATA TRANSFER .............................................................................................................................. 42

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

BATTERY OPERATION ............................................................................................................ 43

4. NANOPHOTOMETER

APPLICATIONS ................................................................... 44

NUCLEIC ACIDS .................................................................................................................. 44

METHOD OVERVIEW ................................................................................................ .......................... 44

MEASUREMENT PROTOCOL ............................................................................................................... 44

CALCULATIONS ................................................................................................ ................................ 48

PROTEIN UV ...................................................................................................................... 54

METHOD OVERVIEW ................................................................................................ .......................... 54

MEASUREMENT PROTOCOL ............................................................................................................... 56

CALCULATIONS ................................................................................................ ................................ 59

PROTEIN ASSAYS ............................................................................................................... 64

METHOD OVERVIEW ................................................................................................ .......................... 64

MEASUREMENT PROTOCOL ............................................................................................................... 65

CALCULATIONS ................................................................................................ ................................ 69

KINETICS .......................................................................................................................... 69

METHOD OVERVIEW ................................................................................................ .......................... 69

MEASUREMENT PROTOCOL ............................................................................................................... 69

CALCULATIONS ................................................................................................ ................................ 71

OD600 .......................................................................................................................... 71

METHOD OVERVIEW ................................................................................................ .......................... 71

MEASUREMENT PROTOCOL ............................................................................................................... 73

CALCULATIONS ................................................................................................ ................................ 75

MORE APPS ....................................................................................................................... 76

MORE APPS: WAVELENGTH ................................................................................................ 76

METHOD OVERVIEW ................................................................................................ .......................... 76

MEASUREMENT PROTOCOL ............................................................................................................... 76

CALCULATIONS ................................................................................................ ................................ 79

MORE APPS: WAVESCAN ................................................................................................... 79

METHOD OVERVIEW ................................................................................................ .......................... 79

MEASUREMENT PROTOCOL ............................................................................................................... 79

CALCULATIONS ................................................................................................ ................................ 82

MORE APPS: ABSORBANCE RATIO ..................................................................................... 82

METHOD OVERVIEW ................................................................................................ .......................... 82

MEASUREMENT PROTOCOL ............................................................................................................... 82

CALCULATIONS ................................................................................................ ................................ 85

MORE APPS: CONCENTRATION ........................................................................................... 85

METHOD OVERVIEW ................................................................................................ .......................... 85

MEASUREMENTS PROTOCOL ............................................................................................................. 85

CALCULATIONS ................................................................................................ ................................ 88

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

MORE APPS: STANDARD CURVE ........................................................................................ 89

METHOD OVERVIEW ................................................................................................ .......................... 89

MEASUREMENTS PROTOCOL ............................................................................................................. 89

CALCULATIONS ................................................................................................ ................................ 92

CUSTOM APPS ................................................................................................................. 93

STORED RESULTS .............................................................................................................. 93

STORED METHODS ............................................................................................................. 94

5. PREFERENCES .................................................................................................... 95

GENERAL .............................................................................................................................. 95

DATE AND TIME ................................................................................................................................ 95

DISPLAY .......................................................................................................................................... 95

ABOUT ............................................................................................................................................. 96

LANGUAGE ................................................................................................................................ ....... 96

ILLUMINATION SAMPLE WINDOW ....................................................................................................... 97

ENABLE NANOVOLUME ..................................................................................................................... 97

DYES …………………………………………………………………………………………………97

WARNING MESSAGES ............................................................................................................ 98

BLANK CONTROL ............................................................................................................................. 98

SAMPLE QUALITY CONTROL.............................................................................................................. 98

NETWORK ............................................................................................................................. 99

WIFI ………………………………………………………………………………………………..100

NETWORK PRINTER ............................................................................................................. 100

REPORT CONFIGURATION ..................................................................................................... 101

6. TROUBLESHOOTING .......................................................................................... 102

SELF-CALIBRATION TEST ...................................................................................................... 102

MESSAGES ......................................................................................................................... 102

IMPORTANT WARNING MESSAGES: .................................................................................................. 102

IMPORTANT ALERT MESSAGES: ...................................................................................................... 104

SYSTEM FREEZE .................................................................................................................. 105

7. ASSISTANCE ..................................................................................................... 106

SUPPORT ............................................................................................................................ 106

REPORT PROBLEM .............................................................................................................. 106

SOFTWARE MAINTENANCE ................................................................................................... 107

RESET ........................................................................................................................................... 107

SOFTWARE UPDATE ........................................................................................................................ 107

CREATE LOG FILE ................................ ................................................................ .......................... 107

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

INSTALL PRINTER DRIVER ............................................................................................................... 108

LEGAL ........................................................................................................................................... 108

TRADEMARKS...................................................................................................................... 108

CONTACT IMPLEN ................................................................................................................ 109

8. MAINTENANCE .................................................................................................. 110

MAINTENANCE FREE TECHNOLOGY ....................................................................................... 110

REPLACEMENT PARTS ......................................................................................................... 110

CLEANING AND GENERAL CARE ........................................................................................... 111

9. WARRANTY....................................................................................................... 111

10. APPENDIX 1- INSTALACE SPEKTROFOTOMETRU ................................................. 112

BEZPEČNOSTNÍ INFORMACE ................................................................................................. 112

VYBALENÍ A UMÍSTĚNÍ .......................................................................................................... 113

11. APPENDIX 2 - INSTALLATIE SPECTROFOTOMETER .............................................. 114

VEILIGHEIDSINFORMATIE ...................................................................................................... 114

UITPAKKEN EN PLAATSEN .................................................................................................... 115

12. APPENDIX 3 - INSTALLATION DU SPECTROPHOTOMÈTRE ..................................... 116

INFORMATIONS DE SÉCURITÉ ................................................................................................ 116

DÉBALLAGE ET POSITIONNEMENT ......................................................................................... 117

13. APPENDIX 4 – INSTALLATION SPECTROPHOTOMETER ......................................... 119

SICHERHEITSINFORMATIONEN ............................................................................................... 119

AUSPACKEN UND PLATZIEREN .............................................................................................. 119

14. APPENDIX 5 – INSTALLAZIONE DELLO SPETTROFOTOMETRO .............................. 121

INFORMAZIONI DI SICUREZZA ................................................................................................. 121

DISIMBALLAGGIO E COLLOCAZIONE ....................................................................................... 122

15. APPENDIX 6 – INSTALACJA SPEKTROFOTOMETRU .............................................. 123

INFORMACJE DOTYCZĄCE BEZPIECZEŃSTWA .......................................................................... 123

ROZPAKOWYWANIE I UMIEJSCOWIENIE .................................................................................. 124

16. APPENDIX 7 – INSTALACIÓN DEL ESPECTROFOTÓMETRO .................................... 126

INFORMACIÓN DE SEGURIDAD ............................................................................................... 126

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

DESEMBALAJE E INSTALACIÓN ............................................................................................. 127

17. APPENDIX 8 – SPEKTROFOTOMETERINSTALLATION ............................................ 128

SÄKERHETSINFORMATION .................................................................................................... 128

UPPACKNING OCH PLACERING .............................................................................................. 129

18. APPENDIX 9 – INSTALAÇÃO DE ESPECTROFOTÓMETRO ...................................... 130

INFORMAÇÕES DE SEGURANÇA ............................................................................................ 130

DESEMBALAMENTO E POSICIONAMENTO ............................................................................... 131

19. ALPHABETICAL APPENDIX ................................................................................. 133

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

1. NANOPHOTOMETER

NANOPHOTOMETER

The Implen NanoPhotometer® spectrophotometer is a mobile, simple to use UV/Visible instrument with a CCD array detector with options ranging from nanovolume to standard cuvette and all-in-one solutions. The N50, N60, and NP80 spectrophotometers measure sample volumes ranging from 0.3 2 µl. The NP80 model also has the capability to measure samples using a standard cuvette. The C40 is a mobile standard cuvette solution with the option to upgrade to accommodate nanovolume measurements.

The NanoPhotometer® runs on a Linux-based operating system (NPOS) that is designed for the use of pre-programmed and custom applications with a high degree of flexibility and processing power.

Sample Compression Technology™ provides easy sample handling which is independent of surface tension. This technology squeezes the sample between two quartz surfaces allowing for unmatched precision and accuracy without the need for dilutions. Combined with our True Path Technology™ the system offers lifetime accuracy and precision without the need for maintenance or recalibration.

Note: It is recommended to use a properly calibrated pipette with high-quality tips to ensure delivery of appropriate sample volumes for nanovolume sample applications.

AT A GLANCE

OVERVIEW

Sample Control™ is a quality control technology that identifies air bubbles, sample impurities, turbidity, lint residues and potential contaminations. Sample Control™ monitors handling characteristics and sample quality in real time to ensure that the measured concentrations are reproducible and most precise.

Blank Control™ gives a warning message for blanks with high background. High background absorbance can be caused by a contaminated blank, blank buffer or by residues from previous users. Insuficient blank readings are the main cause for inaccurate measurements. Blank Control™ will protect the user from wasting time and precious sample on inaccurate readings caused by high background blanks or inappropriate cleaning. For more detailed information see also Technical Note #1 Blank Control™.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Item Number

Touchscreen

Battery Pack

NP80

NP80-Touch + -

NP80-Mobile + +

NANOPHOTOMETER

NP80

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Item Number

Touchscreen

Battery Pack

N60

N60-Touch + -

N60-Mobile + +

NANOPHOTOMETER

N60

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Item Number

Touchscreen

Battery Pack

N50

N50-Touch + -

NANOPHOTOMETER

N50

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Item Number

Touchscreen

Battery Pack

C40

C40-Touch + -

C40-Mobile + +

NANOPHOTOMETER

C40

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

INSTRUMENT REAR PANEL (NP80/N60/N50/C40)

To boot/shut down the NanoPhotometer® click shortly (< 1 second) on the power on/off button. Note: Long clicks (> 3 seconds) initiate a hard reset. Only activate a hard reset of the unit when

necessary. To avoid excess hard reboots, it is recommended to power down the unit from the onboard touch screen by clicking on the power button in the bottom left corner.

INSTRUMENT BOTTOM VIEW (NP80/N60/N50/C40)

Model name, device serial number and FCC ID are located on the identification plate on the bottom of

the instrument.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

ACCESSORIES

STANDARD ACCESSORIES

 Connecting Cable

USB connecting cable to connect the NanoPhotometer® to a computer to control the NanoPhotometer® via computer (please see page 23 Software Installation).

 NanoPhotometer® Power Adapter

Power adapter for the NanoPhotometer® and for charging the optional built-in battery pack. Note: Use only the power adapter supplied with your instrument or a replacement part from the

manufacturer or your supplier.

 Dust Cover

OPTIONAL ACCESSORIES

 Didymium Glass Filter (C40/NP80)

The certified didymium glass filter can be used to verify the wavelength and photometric accuracy of the NanoPhotometer® cuvette applications.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

 Standard Solution (NP80, N60, N50)

The NanoPhotometer® is recalibration free therefore it is not necessary to review the photometric accuracy on a regular basis. If the SOPs of a laboratory require a routine control of the photometric accuracy standard solutions can be used.

Note: Specifications of the standard solution are guaranteed for at least one year. Please see expiration date. Once a vail is opened it can be used for 30 minutes. Note: Please read the Material Safety Data Sheet carefully prior to using this product.

 IQ/OQ documentation (NP80, N60, C40)

The NanoPhotometer® IQOQ package consists of a Standard Solution and/or a Didymium Glass Filter and a corresponding Excel-based software tool depending on the type of instrument. The Excelbased software produces an automated report displaying all relevant data for an audit.

Note: The IQ/OQ documentation is not available for the NanoPhotometer® N50.

 Submicroliter Cell (C40 only)

The submicroliter cell provides the option of upgrading the NanoPhotometer® C40 to a device that handles nanovolume samples as well as cuvette samples. The sample volume range of the submicroliter cell ranges from a minimum of 0.3 µl to 5.0 µl with the path length options including 0.04 mm, 0.1 mm, 0.2 mm, 1 mm and 2 mm which correspond to virtual dilution factors of 1:250, 1:100, 1:50, 1:10, and 1:5 respectively.

 DiluCell (C40/NP80 only)

The DiluCell is a disposable cuvette with shortened path length for virtual dilution of cuvette-based samples. Due to the reduced path length the DiluCell provides an automatic dilution without the need of a physical dilution of higher concentration samples. There are two different sizes of DiluCells available DC 10 and DC 20 which allow an automatic 1/10 and 1/20 dilution of the sample respectively. Bypassing manual sample dilutions reduces dilution errors and cross-contamination making DiluCell ideal for GLP. Combined with small sample volume requirements and bubble free filling, the DiluCell allows for convenient spectrophotometric analysis from 340 - 950 nm.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

 Field Kit

Implen aluminum roller case for safe transportation, designed to fit in most airplane overhead bins; special compartments for all necessary accessories, cleaning tools and samples.

Note: Delivered w/o NanoPhotometer® and accessories Note: Operate the NanoPhotometer® only in the roller case when cover is open. Ensure that air

circulation is possible. Always switch the NanoPhotometer® off for transportation.

 Barcode Reader

It is possible to import sample names from 1D and 2D barcodes. Connect a compatible barcode reader to the USB port of the NanoPhotometer® and click on the sample name input window. After scanning a barcode the sample name will be shown in the input window. The imported name can then be edited or replaced completely.

Barcode readers that have been tested and verified compatible: 1D: Honeywell Voyager 95X0 Single-Line Laser Scanner

Datalogic Touch65

1D & 2D: AGPtEK SC36

Honeywell Xenon 1900g

 DYMO Label Printer

It is possible to connect a DYMO Label printer to the NanoPhotometer® for direct printing on standard or cryo labels. Recommended and tested printers are the DYMO LabelWriter 4XL (label size 10.3 x

15.8 cm) and the DYMO LabelWriter 450 (5.4 x 10 cm). Cryo labels can be printed with both DYMO printers using the following label format: 26 x 12.7 mm and 9.5 mm circle.

Note: After starting the NanoPhotometer, plug in the DYMO printer. The home screen will be shown. Wait at least for 30 seconds for driver installation.

Cryo labels (26 x 12.7 mm and 9.5 mm circle):

Note: Orientation of cryo labels on carrier foil changed from portrait to landscape. Portrait format was discontinued by the supplier.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Printout for DYMO LabelWriter 4XL Printout for DYMO LabelWriter 450

Note: The printouts are optimized for the label size of the DYMO LabelWriter 4XL. All other DYMO printers can be used. However, the font size will be zoomed to utilized paper size.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

 Printer

Printing from the NanoPhotometer® is possible via USB and through network connection. Network printing should be possible with AirPrint® / IPP compatible printers supporting PDF format. Note: IPP version 2.2 is required and some printer configuration settings might need to be changed in order to allow communication with the NanoPhotometer®

The following HP printers have been tested and deemed compatible to print via USB connection:

 HP LaserJet 3030  HP LaserJet m1522nf MFP  HP LaserJet 400 color M451nw

Further HP printers are available upon request. Note: After starting the NanoPhotometer®, plug in the HP printer. The home screen will be shown.

Wait at least for 30 seconds for driver installation.

 HP DeskJet 2543  HP DeskJet 1110

CONNECTIVITY

 USB A

There is a USB A port on the front as well as the rear panel of the NanoPhotometer® which is compatible with standard portable USB 2.0 storage devices for direct data transfer in a variety of formats including Excel. It is also possible to connect a mouse, keyboard, barcode reader, DYMO printer or HP printer directly to the NanoPhotometer®.

Note: We recommend using FAT/FAT32 formatted 2.0 USB flash drives. Encrypted USB flash drives are not compatible with the NanoPhotometer®.

Note: Cordless Bluetooth mice are not supported. Use wired mice only. Note: Connect mouse and keyboard before starting the NanoPhotometer®.

 USB B

There is a USB B port located on the rear panel of the instrument which is compatible with the USB cable provided to connect the NanoPhotometer® to a computer. This USB connection can be used to control the NanoPhotometer® via computer.

 LAN

There is an Ethernet (LAN) connection port on the rear panel of the instrument which enables the NanoPhotometer® to connect with computers within close proximity. This Ethernet connection can be used for data transfer from the NanoPhotometer® to a computer or to control the NanoPhotometer® via a Windows computer. To access the directory of the NanoPhotometer® from a Windows computer via LAN enter the serial number or IP (\\serial number / \\IP) of the NanoPhotometer® in the WindowsExplorer and confirm with the enter key. For Mac OS X open the "Connect to Server" dialog in the "Go" menu of the Mac OS X Finder. Enter the instrument serial number or the active NanoPhotometer® IP address in the server address field to connect.

Note: Plug in the LAN cable before starting the NanoPhotometer®. Note: The maximum LAN cable length is 10 meters. Bit rate is 1 Gbit/s

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

NanoVolume Performance

Detection Range dsDNA

1 ng/µl to 16,500 ng/µl (N50: 5 ng/µl to 7,500 ng/µl)

Detection Range BSA

0.03 mg/ml to 478 mg/ml (N50: 0.15 mg/ml to 217 mg/ml)

Minimum Sample Size

0.3 µl

Photometric Range (10 mm equivalent)

0.02 - 330 A (N50: 0.1 – 150A) Path length

0.67 and 0.07 mm

Dilution Factor

15 and 140

Vortexer

2,800 rpm; tube size up to 2.0 ml

Cuvette Performance

Detection Range dsDNA

0.1 ng / µl to 130 ng / µl

Detection Range BSA

0.003 mg / ml to 3.7 mg / ml

Photometric Range

0 - 2.6 A

Center Height (Z-Height)

8.5 mm

Cell Types

outside dimension 12.5 x 12.5 mm

Heating

37°C ± 0.5°C

 WiFi

The NanoPhotometer® is equipped with WiFi, which can be used as a WiFi network or as a WiFi Hotspot. The WiFi network allows data transfer and direct printing via AirPrint® / IPP compatible printers supporting PDF format.

Note: IPP version 2.2 is required and some printer configuration settings might need to be changed in order to allow communication with the NanoPhotometer®

The WiFi Hotspot provides the option to control the NanoPhotometer® by other WiFi devices like Windows computer, smartphones or tablets.

WiFi Hotspot connection details:

SSID: NanoPhotometer® serial number Password: Implenuser

Note: Due to the limitations of some handheld devices saving to a wireless device is limited to 40

measurements per dataset. Larger datasets can be saved on the NanoPhotometer® itself.

 HDMI

There is an HDMI port located on the rear panel of the NanoPhotometer® which is compatible with HDMI 1.4 cables (or better) to connect the NanoPhotometer® to HDMI compatible monitors.

Note: The maximum HDMI cable length is 5 meters.

NANOPHOTOMETER

SPECIFICATIONS

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Optical Specifications

Wavelength Scan Range

200 – 900 nm (N50: 200 - 650 nm)

Time for Full Scan Range

3.5 – 6.0 seconds

Wavelength Reproducibility

± 0.2 nm (N50: ± 1nm)

Wavelength Accuracy

± 0.75 nm (N50: ± 1.5 nm)

Bandwidth

1.8 nm (N50: 5 nm)

Stray Light

< 0.5% at 240 nm using NaI (N50: < 2%) and < 1% at 280 nm using Acetone (N50: < 2%)

Absorbance Reproducibility

< 0.002 A (0.67 mm path) @ 280 nm (N50: < 0.004 A (0.67 mm path) @ 280 nm)

Absorbance Accuracy

< 1.75 % @ 0.7 A (0.67 mm path) @ 280 nm of the reading

Zero Stability

±0.003 A/hour after 20 min warm up @ 280 nm

Noise

0.002 A rms at 0 A @ 280 nm; 0.002 A (pk to pk) at 0 A @ 280 nm

Optical Arrangement

1 x 3648 CCD Array (N50: 1 x 1024 CCD Array)

Lamp

Xenon flash lamp

Lifetime

109 flashes, up to 10 years

Processing Power and Compatibility

Operating System

Linux based OS

Onboard Processor

Quad Core 1 GHz

Internal Storage

32 GB

Control Options

Onboard with built-in touchscreen, computer, smartphone, and tablet

Software Compatibility

Windows 7 (32 and 64 bit), Windows 8 (32 and 64 bit), OS X, iOS and Android

Min. Requirement Smartphone/Tablet

4 inch screen; Apple: iPad 2, iPhone 5 and iOS 6; Android Phone: OS version 4.4; Android Tablet: OS version 5.0, Quad core 1.2 GHz with 1 GB RAM

General Specifications

Size

200 mm x 200 mm x 140 mm

Weight

3.8 – 5.2 kg depending on configuration

Operating Voltage

90-250 V, 50/60 Hz, 60 W (90 W with battery pack), 18/19 VDC

Display

1024 x 600 pixels; touchscreen glove compatible

Certifications

CE, IEC/EN 61010-1:2012 and EN 61326-1:2013

Battery Pack

optional rechargeable lithium ion battery; 95Wh, 6,6Ah; Operation time: up to 10 h; min. charging cycles: 800

Battery Certification

IEC 62133 and UN38.3 transport test

In- and Output Ports

2x USB A, USB B, HDMI, Ethernet, WLAN

Additional Data Input

Mouse and keyboard options

Security

Slot for Kensington lock

Features and specifications are subject to change without notice. US Patents 20080204755 and 20080106742 Windows is a trademark of Microsoft. Mac OS & iOS are trademarks of Apple, Inc. Android OS is a trademark of Google. Linux is a trademark of Linus Torvalds.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

2. GETTING STARTED

SPECTROPHOTOMETER INSTALLATION

Note: Translations of the Safety Information and Unpacking and Positioning are available in Appendix

1 (Czech), Appendix 2 (Dutch), Appendix 3 (French), Appendix 4 (German), Appendix 5 (Italian), Appendix 6 (Polish), Appendix 7 (Spanish), Appendix 8 (Swedish) and Appendix 9 (Portuguese)

SAFETY INFORMATION

Before commencing installation, please take time to familiarize yourself with warning labels and symbols on your instrument and their meaning. These are to inform you where potential danger exists or particular caution is required. Improper use may cause personal injuries or damage to the instrument. The instrument must only be operated by appropriately trained and experienced personnel. Please read the complete user manual prior to use.

direct current

Do not open the instrument as this can expose the operator to electrical power, UV light, and delicate fiber optics or damage the instrument.

If the submicroliter cell is used with the NanoPhotometer® C40 make sure that the lid is placed on the measurement head prior to the measurement. WARNING: UV exposure when measuring without lid.

Do not use damaged power cords, accessories, and other peripherals with your NanoPhotometer®. Use only the delivered and specified power supply/charger.

Do not expose the NanoPhotometer® to strong magnetic, electrical fields, water, chemicals or any type of liquid as heavy rain or moisture.

Do not put the instrument into fire, as it may swell or explode (battery). Do not store at or use near any type of heat source, especially temperatures above 60°C or in an explosive atmosphere.

Do not leave your NanoPhotometer® on your lap or near any part of your body to prevent discomfort or injury from heat exposure.

Do not place objects on top of the NanoPhotometer®. The NanoPhotometer® with battery pack (mobile version) has to be switched off during transportation.

The on/off button has to be protected during transportation from turning on itself caused by shock or vibration.

Biological samples may contain or have the potential to transmit infectious diseases. Be aware of the health hazard presented by such samples and wear appropriate protective equipment. Handle such samples with the greatest of care and according to applicable regulatory and organization requirements before working with such potential infectious materials. Note: Do not spill any biological samples on instrument components. If spill occurs, disinfect the

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1 instrument immediately following your laboratory protocols and the cleaning instruction of the

instrument (see page 110 Maintenance). The symbol on the product, or on the documents accompanying the product, indicates that this

appliance may not be treated as household waste. Instead it shall be handed over to the applicable collection point for the recycling of electrical and electronic equipment. Disposal must be carried out in accordance with local environmental regulations for waste disposal.

UNPACKING AND POSITIONING

Note: Translations of the Safety Information and Unpacking and Positioning are available in Appendix

1 (Czech), Appendix 2 (Dutch), Appendix 3 (French), Appendix 4 (German), Appendix 5 (Italian), Appendix 6 (Polish), Appendix 7 (Spanish), Appendix 8 (Swedish) and Appendix 9 (Portuguese

Check the contents of the package against the delivery note. If any shortages are discovered, inform your supplier immediately.

Inspect the instrument for any signs of damage caused in transit. If any damage is discovered, inform your supplier immediately.

Ensure your proposed installation site conforms to the environmental conditions for safe operation: indoor use or dry environment. Note: Do not expose your NanoPhotometer® near liquids, chemicals, rain, moisture or dusty environments.

Temperature range 10-40°C; If the cuvette heating is used the range is 10-30°C. If the instrument is subjected to extreme temperature changes, it may be necessary to allow the

instrument to equilibrate. Turn the instrument off and then on again once thermal equilibrium has been established (~2-3 hours).

Maximum relative humidity (non-condensing) of 80% and up to 31°C decreasing linearly to 50% at 40°C.

The instrument must be placed on a stable, level surface that can support 4-5 kg. Ensure that air can circulate freely around the instrument. Confirm while powered on that no materials reduce air circulation. Avoid direct sunlight as it may bleach parts of the instrument and can cause damage to plastic parts.

The equipment should be positioned such that in the event of an emergency the main plug can be easily located and removed.

Always carry the instrument by holding the main corpus of the instrument and not e.g. on the optional attached display or NanoVolume pedestal.

The equipment must be connected to power with the 60W power supply/cord supplied by Implen (90W with battery pack). The power outlet must have a protective conductor (earth/ground). It can be used on 90-250 V, 50-60 Hz power supply system.

For mobile models please load the battery pack at least for 3 hours prior to the first use.

Turn the instrument on using the power button on the rear panel after it has been plugged in. The instrument will perform a series of self-diagnostic checks.

Please contact original supplier immediately if technical or sample handling difficulties are experienced.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1 Note: If this equipment is used in a manner not specified or in environmental condition not suitable for

safe operation, the protection provided by the equipment may be impaired and the instrument warranty voided.

SOFTWARE INSTALLATION

NPOS OVERVIEW

NPOS is a Linux-based operating system designed for the NanoPhotometer®. NPOS can store data either to a common directory or be configured to save to independent

directories according to file format and/or instrument. NPOS can save data in an Implen .IDS format, PDF or as an Excel format file. Note: PDF and Excel files cannot be opened on the NanoPhotometer®. The files need to be

transferred to a computer or device where Excel or a PDF reader is installed. Note: Please do not connect the instrument to a computer until the NanoPhotometer® NPOS software is installed on the computer.

REQUIREMENTS AND COMPATIBILITY

The NPOS user interface is designed that all features can be operated by using a touchscreen. If the software is installed on a computer without touchscreen, the user interface can be operated by using a keyboard and a mouse. Before starting the installation process ensure the device is compatible with the requirements for installation.

Compatible Control Devices Computer:

PC: Windows 7 / Windows 8 (32 bit or 64 bit) Mac: OS X

Tablets (minimum requirements):

iPad 2: iOS6 Android (quad core 1.2 GHz with 1 GB RAM): Android version 4.4

Smartphones (minimum requirements):

iPhone 5: iOS6 Android (quad core 1.2 GHz with 1 GB RAM): Android version 4.4

Windows is a trademark of Microsoft. Mac OS & iOS are trademarks of Apple. Android OS is a trademark of Google. Linux is a trademark of Linus Torvalds.

Note: There are two user interfaces of the software available; one for built-in touchscreen, computer and tablets and one for smartphones.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

INSTALLING SOFTWARE ON COMPUTER

The NanoPhotometer® software can be installed on compatible PC and Mac computer systems. Various operating systems and PC hardware may cause the set-up procedure to differ from that described here. This process is given as guidance only; it may need adaptation for other systems.

Note: Do not connect the NanoPhotometer® to the PC/Mac before NPOS installation. Note: If a previous version of the NPOS software is already installed on the PC, remove the USB cable and uninstall the NPOS software before installing the new software version. Note: The Windows and Mac installation file is provided on the Implen USB flash drive which is

included with the NanoPhotometer® at time of delivery. The files are always available for free download in the download section of the Implen webpage.

 NPOS installation for single user on Windows computer

1. First update the firmware of your NanoPhotometer® (NPOS.bin) before starting the update on your computer.

2. Launch the NPOS installation file (NanoPhotometer®.msi) and follow the installation routine. The installer should be logged in with full administration rights. If you have insufficient privileges, installation may fail. If in doubt consult your PC administrator.

3. Open the NPOS software and choose the preferred connection. For USB, connect the NanoPhotometer® with the provided USB cable to the PC, for WiFi Hotspot connection ensure stable WiFi connection between PC and the NanoPhotometer® WiFi Hotspot (SSID: Serial number, password: Implenuser) and for LAN connect the NanoPhotometer® to the local network via Ethernet cable or WiFi network.

Note: If there is Avira installed on your computer, it is recommended to switch off the browser safety. This may interfere with the NPOS running on your computer.

 NPOS installation for multi-user on Windows computer

1. Update first the firmware of your NanoPhotometer® (NPOS.bin) before starting the update on your computer.

2. Launch the NPOS installation file (NanoPhotometer®.msi) and follow the installation routine. Choose on the License Agreement dialog the option “Advanced” and on the following dialog “Install for all users of this machine”

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

The installer should be logged in with full administration rights. If you have insufficient privileges, installation may fail. If in doubt consult your PC administrator.

3. Open the NPOS software and choose the preferred connection. For USB, connect the NanoPhotometer® with the provided USB cable to the PC, for WiFi Hotspot connection ensure stable WiFi connection between PC and NanoPhotometer® (SSID: Serial number, password: Implenuser) and for LAN connect the NanoPhotometer® to the local network via Ethernet cable or WiFi network.

Note: If there is Avira installed on your computer, it is recommended to switch off the browser safety. This may interfere with the NPOS running on your computer.

INSTALLING NANOPHOTOMETER

The NanoPhotometer® App can be installed as an application on tablets and smartphones with compatible Android and iOS operating systems. The NanoPhotometer® App is available for free download in the app store (Apple Store and Google Play Store).

1. Download and install the NanoPhotometer® App from the app store

2. Connect the tablet or smartphone via WiFi Hotspot to the NanoPhotometer®. SSID: Serial number, password: Implenuser

3. Open the NanoPhotometer® App

4. When connected via WiFi Hotspot the NanoPhotometer® will recognize the tablet/smartphone as a remote control device and measurements can be initiated from the tablet or smartphone.

5. Results will be shown on the tablet or smartphone once measurements have been taken

APP ON TABLET OR SMARTPHONE

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1 Note: In order to install the NanoPhotometer® App on a tablet or smartphone, the device must have

an established internet connection to access the app store for app download. Note: The version of the app and the software of the NanoPhotometer® should be the same. Different versions may have not full functionality.

FIRST STEPS AND CONFIGURATION WIZARD

When starting the Implen NPOS the first time an Implen configuration wizard is shown. Please accept the End User License Agreement (EULA) and select the country in which the NanoPhotometer® is used and confirm.

PRINTER INSTALLATION

For NanoPhotometer® with software version 1.1.11147 or higher NO driver installation is necessary.

1. Switch NanoPhotometer® on / home screen

2. Connect DYMO/HP printer via USB cable

3. DYMO/HP printer is ready to use after 30 seconds

For NanoPhotometer® with previous software versions printer driver installation is necessary.

1. Switch NanoPhotometer® on / home screen

2. Update to newest firmware version

Update Process:

a. Copy NPOS.bin to a USB flash drive into the root folder b. Insert the USB flash drive into your NanoPhotometer®

c. Select: “Assistance / Software Maintenance” d. Click on “Update”

e. Wait until the NanoPhotometer® reboots

Note: Always update the firmware on the NanoPhotometer® first, then update the client software (PC/Mac, tablet, smartphone).

3. Go to “Assistance / Software Maintenance”

4. Start driver installation by a click to the “Install Driver for Printers” button

5. Wait until the following message is shown: “Installed driver for printer successfully.”

6. Go to home screen

7. Connect DYMO/HP printer via USB cable

8. Wait for 30 seconds

9. DYMO/HP printer is ready to use

Note: In order to connect a printer to the NanoPhotometer®, please do so from the Home Screen. Printers will not properly function if USB connection of printer is done from within a method. Always return to the Home Screen before connecting a printer.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Method Icons

Description

Nucleic Acids

Concentration, purity, and dye incorporation for DNA, RNA, Oligo, and other nucleic acids

Protein UV

Protein UV determination at 280 nm (or in a range of 200 330 nm), purity and dye incorporation

Kinetics

Time vs. Absorbance readings

3. NANOPHOTOMETER

The NanoPhotometer® product line offers a complete solution for Nanovolume (NP80/N60/N50/C40) and standard cuvette (C40/NP80) applications. With the NanoPhotometer® NanoVolume applications, sample volume ranges from a minimum of 0.3 μl to a maximum sample volume of 2.0 µl (5 µl for using the submicroliter cell/C40). Standard cuvette applications can be performed with 10 mm, 5 mm, 2 mm, 1 mm and 0.5 mm path length quartz, glass, or plastic cuvettes with a center height of 8.5 mm.

BASICS

APPLICATIONS OVERVIEW

The NanoPhotometer® comes with pre-programmed applications as well as the ability to create custom applications. To select a method click/tap on the corresponding icon and the method will open immediately.

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Protein Assays

BCA (562 nm), Bradford (595 nm), Lowry (750 nm), and Biuret Assays (546 nm)

OD600

Measures cell density at 600 nm (or in a range of 200 – 900 nm)

Stored Results

Archive of stored results

Stored Methods

Collection of stored custom methods

More Apps

Additional applications found on a secondary method screen

Wavelength

Define one or multiple wavelength between 200 – 900 nm (N50: 200 – 650 nm) for absorbance measurements

Wavescan

Define desired full scan range anywhere between 200 - 900 nm (N50: 200 - 650 nm)

Concentration

Define extinction coefficient for automatic concentration calculations

Absorbance/ Ratio

Define two wavelengths absorbance/ ratio calculation

Standard Curve

Create a standard curve at a defined wavelength

Custom Apps

Optional custom applications for personalized methods tailored to individual spectroscopy needs

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

Icon

Name

Action

WiFi Network

WiFi network active; status of WiFi connection

WiFi Hotspot

WiFi hotspot active

Assistance

Opens the assistance page

Battery Status

Shows the actual battery status (only shown with optional battery pack)

Preferences

Opens the preferences page

Home Screen

Returns to the home screen with application icons for method selection.

Leave Method

Returns to the previous application selection

Back

Returns to the previous page (smartphone only)

Next/Confirm

Confirms parameter and opens the next screen (smartphone only)

Delete

Deletes added functions in parameter; empties input windows

Delete Data

Opens a delete dialog pop up

Email Data

Opens an email dialog pop up (computer and tablet versions only)

Save Data

Opens a save dialog pop up

Print Data

Opens a print dialog pop up (only shown when DYMO/HP printer is successfully installed and connected)

Add Folder

Adds a new folder to the directory

Manage Data

Opens a dialog pop up with several action options including delete, rename or import folders/files/data as well as copying or move folders/files/data to defined directories

Store Method

Opens a dialog pop up with the possibility to store the actual method parameter to a custom method

Full Scale

Restores graph to original size without zoom

Cancel

Returns to the previous screen without implementing any changes

ICONS

NanoPhotometer® NP80/N60/N50/C40 User Guide Version 3.1.1

BUTTONS

Prior to any sample measurement at the start of a new method a blank

measurement of either water or the buffer of the samples is required to give the NanoPhotometer® a reference of what zero should be. It is recommended to re-apply the blank and measure it as a sample to ensure the graph of the blank spectra is a flat line.

To initiate the spectral scan of the sample press the sample button. The

data will be temporarily stored until the method is exited; at this time the user needs to define if the samples should be saved or deleted.

The Change to Cuvette button (NP80) is located at the bottom of the

parameter window. The change to cuvette/nanovolume button toggles between nanovolume and cuvette applications. Selecting the Change to Cuvette button activates the cuvette compartment (opens the sliding cover and switches the red arrow LED on). Only cuvette measurements are possible when this mode is selected. Selecting the Change to NanoVolumebutton while the cuvette port is activated will deactivate the cuvette compartment (closes the sliding cover and switches the LED off) and only nanovolume measurements are possible.

Note: Please make sure that the nanovolume arm is closed during cuvette measurements.

The Change to NanoVolume button (C40) is only shown when submicroliter

cell applications are enabled in the preferences. The Change to NanoVolume/Cuvette button toggles between cuvette and submicroliter cell applications. With the NanoVolume submicroliter cell application the parameter of each method shows a list for lid selection (Lid 5, 10, 50, 100 and 250). Choose the correct lid and the results are automatically calculated considering the virtual dilution.

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Implen NanoPhotometer NP80, NanoPhotometer N60, NanoPhotometer C40, NanoPhotometer N50 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual