IEC Centra-CL3, Centra-CL3R Operation Manual

IE<:

OPERATION·.··

MANUAL

OM3750

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IEC

INTERNATIONAL

300 SECOND

TEL.

(781) 449-8060

AVENUE,

EQUIPMENT

NEEDHAM HEIGHTS, MA 02194

•TOLL

FREE (800) 843-1113

COMPANY

U.SA

•FAX

(781) 444-6743

1 Introduction

1.1

General Product Description

Centra-CL3 series units are high-speed, multipurpose centrifuges, used applications.

The CL3 Series (Centra-CL3) and refrigerated (Centra-CL3R). Sections this manual that apply designated Centra-CL3R

Both units accommodate a wide variety fixed angle, swinging bucket, and fixed horizontal designs. They can process tubes, bottles, microplates, microcapillary tubes, cytological slide carriers, and microsample tubes. The units can centrifuge up to one liter

operation.

Each centrifuge has an easy to use front panel that provides two modes

of

operation: Manual and Programmed.

in

medical, industrial, and scientific

is

available in two versions: ventilated

to

the refrigerated version will be

or

refrigerated only.

of

rotors, including

of

fluid in a single

of

f

l

Manual mode speed/force, and time values for individual runs.

Program mode allows you to define and save a maximum ninety-nine specific sets reuse.

Centra-CL3 Series Operation Manual

is

used for entering temperature (CL3R only),

of

run parameters, to recall and

1

of

TABLE

OF

CONTENTS

I

t

.Centra-CL3 Series Operation Manual

.Chapter 1: Introduction

1.

1 General Product Description ............................................................................... 1

1.2 About this Manual .............................................................................................. 2

1.3

Warnings, Cautions,

Chapter 2: Installation

2.1

Receiving the Unit .............................................................................................. 5

2.2 Site Preparation .................................................................................................. 5

2.3 Power Configuration ........................................................................................... 7

2.4 Moving the Unit .................................................................................................. 8

2.5 The Front Panel .................................................................................................. 8

. Chapter 3: Operation

3.

1 Rotor and Accessories ......................................................................................

3

.2

Starting and Stopping a Run .............................................................................

3.3 Stored Programs ............................................................................................... 20

3

.4

Centra-CL3 Series Refrigeration .......................................................................

3.

5 Diagnostic Messages and Error Codes ..............................................................

and

Notes ........................................................................... 3

13 17

21 23

Chapter 4: Applications

4.1

Introduction ......................................................................................................

4.2 Speed and Force Tables .................................................................................... 27

4.3

Derating for Dense Samples Table ....................................................................

4.4 Chemical Resistance Table ................................................................................ 39

4.5 Decontamination Table ..................................................................................... 40

4.6 RCF Nomograph ..............................................................................................

Chapter

5.1

5.2 Care and Cleaning .............................................................................................

5.3

5.4 Condition

5.5

Chapter 6: Specifications

Unit Specifications ................................................................................................... 49

5:

Maintenance

Introduction ......................................................................................................

Cover Interlock Bypass Switch ......................................................................... 46

of

Returned Equipment ..................................................................... 47

Warranty ........................................................................................................... 47

Centra-CL3 Series

Operation

I

Manual

25

36

41

43 43

,!

..

~·,

. "

. t

The CL3 Series features a maintenance-free, brushless

motor, and an easy-to-use front panel, which provides three versatile timing modes: automatic timed run, momentary spin, and continuous operation (hold mode). Acceleration and brake rates may be controlled, for fast separations or slow for delicate samples. Repeat runs, with the same speed and time settings, may be achieved at the touch

A fail-safe cover interlock insures that before a run can begin, and keeps the cover closed, until the

rotor has reached a safe low speed (below 100 rpm), even in

the event

The rugged steel cabinet and rigid construction provide

quiet operation and long term reliability.

1.2 About This Manual

of

a power failure.

of a key.

to

optimize runs: rapid

the

cover is closed,

!

;

I

The Operations Manual contains all needed to install, operate, and maintain a CL3 Series centrifuge. Refrigerated and ventilated models operate similarly, and any differences are highlighted and noted, throughout this manual. This manual, also, contains speed and force, derating, chemical resistance, and decontamination tables. The last chapter lists the units' specifications.

This manual to operation information, it contains a few basic troubleshooting techniques, and a chapter on maintenance. This Operation Manual centrifuge units.

is

written for centrifuge operators. In addition

is

not a guide for servicing

of

the

information

Centra-CL3 Series Operation Manual

2

1.3 Warnings, Cautions, and Notes

The tenns warning, caution, and

in

meanings

Warning

• A that could result

Caution

• A

this manual.

advises against certain actions

in

personal injury.

advises against actions

note

have specific

or

situations

situations that could damage equipment, produce inaccurate data, or invalidate a procedure.

• A Note provides useful infonnation regarding an operation, function,

or

procedure.

Centra-CL3 Series Operation Manual

3

- !

t

-

2.1

Receiving the Unit

International Equipment Company (IEC) ships the centrifuge Follow the unpacking instructions on the carton. complete and return the postage-paid warranty card.

2 Installation

in

a carton that protects it from shipping hazards.

Be

sure

to

2.2

·

Site Preparation

The unit normally resides on a bench top. The Centra-CL3

in

(ventilated model) can be placed than 0°C), for processing temperature-sensitive samples. When you remove the centrifuge from a cold environment, do not operate for a minimum condensation will evaporate.

Note: When used

noise may become evident. The bearing lubricant thickens at low temperatures. thinned and distributed more evenly. Once this occurs, any noise should subside.

in

a cold room environment, some bearing

As

the centrifuge speeds up, it

a cold room (no colder

of

two hours, so that any

is

Centra-CL3 Series Operation Manual

s

The following table lists the physical dimensions for Centra-CL3 and Centra-CL3R:

the

CL3R

Sample

Cover

Width

Depth

A clearance

side centrifuge on a clean, dry surface, suction feet at the area beneath the unit free

The resting surface must free operation. improperly loaded centrifuge may vibrate

Closed

Open

of

the unit,

Loading

Height

of

8 cm (3 inches) should

to

ensure proper ventilation. Place

bottom

A rigid and stable location is important. An

12.5" (32 cm) 12.5" (32 cm)

14.5" (37 cm) 14.5" (37 cm)

33" (84 cm)

26.5" (68 cm) 19.25" (49 cm)

21" (53 cm) 21" (53 cm)

be

to

make certain that

grip the surface firmly. Keep

of

debris and loose materials.

be

level,

to

ensure quiet, vibration-

CL3

33" (84 cm)

provided on each

the

or

move.

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Centra-CL3

Series

Operation

6

Manual

2.3

Power Configuration

Power Cord

The CL3 Series uses appropriate for use throughout the world. Please check catalog number ensure that the machine you have is the proper power configuration. For best results, the refrigerated centrifuge, Centra-CL3R, should be used Variations in line voltage speed and other characteristics. Less than nominal line voltage may prevent the centrifuge from reaching maximum published specifications Power line voltage, at some locations, may sag, when refrigeration system turns on.

The unit requires a grounded power supply

If

outlet). outlets, arrange for a proper grounding. The power plugs in on the left rear side

your facility does not have grounded

AC

power in different configurations,

of

the model that you have purchased,

on

a dedicated line.

or

frequency affect the

of

speed and/or temperature.

(3

prong

of

the unit.

the

to

unit's

the

power

cord

~=~o~=:i:ili~~~t~~~e

power plug tbatdoes not

have

~

gr~Undilig

pin.

··

·.···

· ·

Circuit Breaker

The

power

highest

interchanged demand.

createa

The system provides emergency situations, such as power surges, that could damage the unit.

If

the circuit breaker trips:

1.

Unplug the unit.

2.

Press the white button, on the left side

3.

Plug the unit back

cord

current

with

Exchange

fire

hazard.

provided with the tiJlltiscorrectly rated

demand.

cords

of

This

poweirord.shouldJ1ot

from

equipinentwitb.Jower current

power

cords

befiVeell

an

automatic circuit breaker, for

in.

equipment•may

of

the unit.

be

forthe

Centra-CL3 Series Operation Manual

7

2.4 Moving the Unit

Suction cups, at the bottom the work surface. Keeping the unit stationary is a safety feature.

To move the unit to a new location:

1.

Check that the new site meets the criteria

2.2., before moving the unit.

2.

Position a flat object, such as a tongue depressor, near a suction cup at the bottom

3.

Lift up enough to break the vacuum seal.

4.

When work surface.

an

edge

of

all

four cups are disengaged, lift the unit from the

Caution: When the unit

the suction cups adhere correctly to the work surface.

of

the unit, keep it anchored

in

Section

of

the unit.

the cup, and insert the flat object far

is

in

its new location, ensure that

to

2.5 The Front Panel

©

The Front Control Panel

(Centra-CL3R)

Centra-CL3

Series

Operation

8

Manual

©

On/Off

use control panel and refiigeration system (Refrigerated model only). The Stop a run with the display chamber temperature whenever they are plugged in, but will not cool down light indicates that the centrifuge is plugged

key:

The On/Off key must be activated,

of

the front panel. This key applies power

On/Off key is inoperative during actual runs.

STOP key. Refrigerated models

if

the unit

is

off

to

enable

to

the

The red STOP

in.

The control panel contains numeric displays for (Speed/Force), Time, and Temperature (Refrigerated only). These displays have display) and Set (dim display).

In Actual mode (bright display), they indicate current run

conditions, such as:

• rotor speed

• elapsed time of,

• actual temperature (Refrigerated only).

In Set mode (dim display), the display indicates the desired

settings for the run. Set mode

• whenever you use the up and down arrows

• briefly, at the start

• briefly, after the unit

The display The display numeric displays can, also, display warning messages (see Section 3 .5). Descriptions appear on the following pages.

is is

two

states

or

force

or

time remaining

of

is

bright, when it shows Actual run conditions.

dim, when it shows Set parameters.

or

modes: Actual (bright

is

operative:

a run

switched ON

in,

the run

RPM/RCF

or

error

of

the displays

The

Centra-CL3 Series Operation Manual

9

0

Speed/Force display: The number in the speed/force

rotor

speed

display (above this symbol) represents the

RPM

or

force in RCF. Press this symbol

RPM

and RCF. When indicates revolutions per minute. When the display indicates relative centrifugal force.

to

arrow keys shows speed within 50 RPM. It never requires calibration. Select speed in increments through 8,500 RPM. Select RCF in increments from

1 - 1,000 xg by 50 xg and above 1,000 xg by 100 xg. The numeric display can, also, display warning messages (see Section 3.5).

change the set speed

RPM

is selected, the display

of

100 RPM, from 1,000

or

to

toggle between

RCF

is selected,

force.

or

The

error

Use

in

the

display

Time display: The number

symbol) indicates time. Below ten minutes, time is

displayed as minutes:seconds. Above ten minutes,

minutes are displayed.

Time is set

• 1 second increments, from 1 15

•

• 1 minute increments, from 5 - 99 minutes.

In normal timed mode, the system counts point. In time counts up.

In At-Start mode, the timer begins counting run. In At-Speed mode, the timer begins counting when the rotor

Temperature display: The number

this symbol) represents temperature from - 9 °C

in:

second increments, from 1 - 5 minutes.

Hold

or

reaches

95%

of

through+

in

the display (above this

59

seconds.

down

momentary spin modes,

at

the set speed.

in

the display (above

in

degrees Celsius,

40 °C (Refrigerated only).

just

from the set

the

system

the

start

of

a

Centra-CL3 Series Operation Manual

10

:I

Program

key: This key saves the currently displayed

desired settings as stored programs I through 99 (see Section 3.3). The numeric display (above this symbol) shows the stored program number and mode

of

operation

(see Section 3 .2).

Rotor/Radius indicates the selected rotor number

Key: The display (above this symbol)

or

the

rotor radius,

in centimeters. The rotor/radius key toggles between the two displays. The applicable IEC rotor numbers are supplied

in

the memory, along with their maximum radii, in

centimeters. To select a rotor number, toggle

to

ROTOR, and press an arrow key under the rotor/radius display. To change the radius, toggle to RADIUS, and press an arrow

Note

key under the rotor/radius display.

that the radius cannot be changed to a radius larger than the maximum radius,

or

less than the minimum radius, for the selected

rotor.

Use the arrow keys to view or change the Set parameters for Speed/Force, Time, Temperature (Refrigerated only) Rotor/Radius,

or

Program. The first time the key is

pressed, the numeric display switches from Actual readings

If

to Set parameters, without changing them. key a second time, the selected parameter increases

decreases once for each depression.

will

down, the setting

keep changing, until you release the

If

you hold the key

you press the

or

key.

The longer you hold the key, the more rapidly the setting changes. Hold a key down, to approach a desired setting. Then, press the up or down key, repeatedly, to select the exact setting. When you release the arrow keys for 3 seconds, the display returns to the Actual readings.

Centra-CL3 Series Operation Manual

11

l

Acceleration and Braking

Gentle acceleration and braking can be selected, when

centrifuging delicate samples. The gentle settings avoid mixing

This key controls rotor acceleration up to 400 RPM. yellow light over the rabbit is lit, selected. acceleration 3 5 seconds to achieve 400 RPM, depending on the rotor and its contents. After 400 RPM,

applied, until the set speed is reached.

This key controls rotor braking.

rabbit

the turtle

rotor

out,

operating speed to a stop.

This key starts a run. A run is governed by the Set

parameters (manual

light blinks, until the rotor reaches 95% speed. The light stays on until the end

of

density gradients

If

the yellow light over the turtle

is

selected. Slow acceleration takes from

is

lit,

full

braking

is

lit,

slow braking is selected. (This means the

will

coast down from 600 RPM.)

all

braking is disabled; and the rotor

or

breakup

full

If

is

selected.

programmed). The associated green

of

pellets.

If

acceleration

is

full

acceleration

the yellow light over the

If

the yellow light over

If

both lights are

will

of

the set run

of

the run .

is

lit, slow

15

is

coast from

the

to

l

}

I

l

1

•

This key stops a run. (A run will, also, stop automatically when the set time has elapsed momentary mode.) The red light will flash, to indicate the rotor

is

still slowing down (braking run ends, the red light stays on, indicating that the rotor has stopped.

This key unlocks the cover. This key

is

in

progress. Pressing it will not stop the run. The cover

will not unlock, until the rotor has slowed to below 100

rpm. The cover will open automatically, on ventilated units only, at the end

of

a run.

or

the start key

or

coasting). When the

is

released, in

inoperative

if

a run

Centra-CL3

Series

Operation Manual

12

3 OPERATION

3.1

Rotor and Accessories

A balanced unbalanced load produces vibration, and can damage the unit. A 2 gram load imbalance, at a speed imparts force equivalent to Always ensure that the rotor is loaded symrn.etrically, with a full

complement

tubes. Tube adapters should also be installed symmetrically.

IEC rotors are dynamically balanced at the factory. IEC

matches removable parts (trunnion rings, shields, buckets, and carriers) to within I gram, and stamps the weight on each piece. Check these markings, whenever you interchange parts, to ensure that opposite parts are matched. Ensure that the total weight parts, loaded within 1 gram. The position numbers, present on many rotors and adapters, identify opposing tube positions.

To

obtain good dynamic balance, opposite loads must not only be equal of

gravity. Opposing containers must be alike thickness, and distribution especially important for large containers.

lo.ad

is

essential for all centrifuges.

9.

I kg (20 pounds) at rest.

of

accessories, and a

of

samples and removable

in

opposing positions, are equal

in

mass, but must, also, have the same centers

of

glass

full

or

plastic. This

An

of

4600 RPM,

(or paired) set

in

weight, to

in

of

shape,

is

Centra-CL3 Series Operation Manual

13

I

Tubes loaded into swinging bucket symmetric, around the

ax.is

of rotating the entire rotor 180°, in

the same apparent positions (not mirror images). In

rotors

must be rotation. Verify this by by

hand.

The

loads should be

addition, the loads within each bucket must, also, be

symmetric around the bucket's pivot axis. Verify this ensuring that each bucket

from the vertical, when the rotor is

balance within each bucket ensures that tubes swing out to horizontal, when

is

loaded so that it does not tilt

at

rest. Maintaining

the

bucket and the

the

rotor reaches

by

operating speed, applying centrifugal force toward the

of

bottom

causes vibration and premature wear

the tubes. Failure to achieve full swing-out

of

the rotor and the

motor.

Samples oflike (similar) specific gravities may be processed in

the same run, provided that the samples

of

the same type

are balanced around the rotor, as though they were the only

in

pairs

the rotor.

I

Rotor Balance

Load tubes

1.

Load two tubes at positions:

2. Load four tubes at positions:

3.

Load six tubes at positions:

4.

Loading

recommended.

in

the following manner:

9

and

18.

1,

5 and 10,

7, 3 and 16,

1,

9, 5 and 14, 18,

7, 9, 3 and 12, 18,

an

14

or

12

or

odd number

10

16

of

tubes is not

Caution:

Do

not exceed maximum rated speed for each

rotor/accessory combination. Maximum rated speeds can

in

be found

Section 4.2 - Speed

And

Force

Tables.

I

r -

Centra-CL3 Series Operation Manual

14

V~ration

(

All

centrifuges have critical speeds, at which vibration occurs. vibration will cease. This inherent condition, also, during deceleration. An imbalanced load intensifies

As

the

speed increases, beyond

the

critical speed,

occurs

these

critical vibrations. Do not continuously operate this

centrifuge at observed critical speeds.

Rotor Installation

To

install the #243 rotor:

1.

Press the disk (located

on

the

underside

of

the

rotor)

the rotor.

2. Slide the

Release

rotor

the

over

disk.

the shaft, until it snaps into place.

3. Pull the rotor straight up, to insure a positive lock.

3.

PULL TO

CONf

0

STRAIGHT

IRM

UP

ON

SECURE

ROTOR

ATTACHMENT

0

to

I.

PRESS

AND

Rotor Removal

UP

HOLD

HERE

To remove the 243 rotor:

1.

Press the disk (located on the underside the rotor.

2.

Lift the

3.

Release the disk.

Centra-CL3 Series Operation Manual

rotor

15

straight up.

2.

SLIDE AND

RELEASE

ONTO

SHAFI

of

the

rotor)

to

Rotor Adapter

To install the shaft adapter for an existing rotor:

1.

Press the adapter disk, and slide the adapter over the rotor shaft. Release the disk.

2.

Pull the adapter straight up, to insure a positive lock.

3.

Align the rotor keyway with the key on the shaft adapter, and place the rotor onto the adapter.

4.

Tighten the rotor locking nut (with the provided wrench), until it tightly engages the rotor.

To remove existing rotors from the shaft adapter:

1.

Remove any sample tubes, shields, buckets, and other

accessories, from the rotor.

2.

Unscrew and remove the locking screw.

3.

Grasp the rotor, and rock

it

front-to-back, and side-to-

side.

4.

Remove the rotor from the shaft.

Centra-CL3

Series

Operation

16

Manual

3.2 Starting

and

Stopping a

Run

Read Section 2.5, for a general description panel. The settings displayed on the front panel always govern the operation above the Program icon, shows the unit's operating mode.

It is important that the unit be in the correct mode for the

desired operation.

These digits and symbols are displayed above the program icon. They can be any

blank The unit

1-99 The unit

program number.

C The unit

program discussed

The display indicates the last parameters selected for each Program mode operation.

of

the unit. 'f he number

of

the following:

is

in

manual operation.

is

under control

is

set to Rapid Condition, a special

of

in

Section 3.4.

the displayed stored

of

the front

or

symbol,

Centra-CL3

Series

Operation

17

Manual

Manual Operation

For

manual operation, set the mode so that the display

above the Program icon

is

blank. Select the desired temperature (CL3R only), speed/g-force, run time, acceleration mode, and braking mode. Press START, to start the spin.

The minutes display counts down, and displays the time remaining

in

the current spin, during manual operation.

The specified run time begins when the pressed,

or

when the rotor reaches 95%

START

of

key

is

set speed (see Timing Mode). Braking begins when the elapsed time reaches this desired setting. Run time does

not include

braking time.

will

The spin

stop automatically, at the end interval. A run can, also, be stopped, at any time, pressing the

STOP

key.

of

the desired

by

The settings can be changed during a manual run. These changes affect the run

progress.

If

the time setting is

in changed, during a run, the unit adjusts the count-down timer, to display the revised setting as the total time

If

run. the run

the new time selected

will

end.

is

less than the elapsed time,

of

the

The unit's mode (settings) cannot be changed during a program mode spin.

Centra-CL3

Series

Operation Manual

18

- j

. Timing Mode

Four timing modes are available on these units: timing mode, press the down arrow, under the time display, until the time goes below zero, and the appropriate symbol

is

displayed.

Momentary easily separated samples, for simultaneous mixing

samples, and

·of

the tube.

For

momentary spin, set the mode so that three dashes (---) appear above the Clock icon. Select temperature only), speed/g-force, acceleration mode, and braking mode, as for manual operation.

Press and hold the

press the key, and ends when you release the key. In this

mode, you can perform very quick separations

During a momentary spin, the unit displays actual values,

not desired settings. The time display counts upward, and displays the elapsed time since the pressed.

Mode

to

(--)

- Momentary spin is useful for

deposit condensate droplets at the bottom

START

key.

The run starts when you

START

To

of

(CL3R

or

protocols.

key was

select a

Hold

Mode preset time limit), set the mode so that Time display.

Select temperature (CL3R only), speed/g-force, acceleration mode, and braking mode, as with manual operation. Press and release the the

key,

Hold mode

setting display counts upward, and displays the elapsed time spm.

At-Start

down pressed). The unit

At-Speed down, when the rotor has reached 95% display acceleration to 95%

(HLd) -For

START

and stops only when you press the

is

like manual operation, except that the time

is

not used. During a run

Mode

at

the beginning

will

(ACC) - The set time

is

Mode

(SPd) - The set time

alternately show the set time and SPd, during

hold mode (operation without

HLd

appears in the

key. The run starts when you press

STOP

in

hold mode, the time

will

start counting

of

acceleration (when the start key

originally set to this mode.

will

start counting

of

set speed. The

of

the set speed.

key.

of

the

is

Centra-CL3 Series Operation Manual

19

To

select a timing mode, press the Time

and scroll below 0 minutes.

appear. Press and release the Time

choose the desired mode. After three seconds, pressing the return key has not been pressed.

PROGRAM

to

the last selected run time, provided

ACC,

or

START

SPd,

down

keys,

down

HLd,

arrow

the

arrow

or

key,

- will

key, to

or

after

display will

up

arrow

The timing mode is stored along with when a program is saved. recalled, altered, and not re-saved, revert back

3.3 Stored Programs

The CL3 Series has an internal memory capable

99 sets and can be recalled by selecting a program number (from

is turned for a particular run change the unit's program, rotor/radius, during a spin.

Lock

Program

Programs can be locked by scrolling and pressing the scroll program display, after the program number is displayed. unlock a program, scroll to the desired locked

the

other

If

a saved program has been

the

timing

to

the previous mode,

of

run parameters. Each set,

1-99). Programs are retained in memory, even

off

When necessary, a program can

or

changed permanently.

PROGRAM

to

a locked program, the letter L will flash in the

press the locked programs cannot be changed.

PROGRAM

key three times. Parameters

when

key three times.

recalled.

or

program, is stored

or

timing modes,

to

the

desired program,

You

parameters,

mode

will

of

holding

if

the

power

be

modified

cannot

When

program

of

you

To

and

Recall

Program

Press a program arrow key, program number.

The program's set parameters will START,

Centra-CL3

to

begin this run.

Series

Operation

20

to

Manual

select the appropriate

be

displayed. Press

Add/Change Program

Select a program number with the program arrow keys. current program parameters

will

appear on the display. Modify the desired parameters, using the parameter keys, or the ACCEL

or

BRAKE keys. The program .

The

arrow

number will flash, indicating that the program was changed, and has not, yet, been saved. Make the changes permanent by pressing the Program Save (file folder) key. The program number will stop flashing, and the new program settings will be displayed. The program will remain in memory, until further changes are made.

To make changes temporary, press

pressing the PROGRAM SA VE (file folder) key. The program display will flash, to indicate that the values are now stored is

not currently operating from program mode. The original

in

program will remain unchanged, as long as the PROGRAM SA VE (file folder) key is not pressed.

· 3.4 Centra-CL3R Refrigeration

Refrigerated units refrigerate the rotor chamber whenever the cover Refrigeration

chamber to the currently displayed temperature setting. you use the keyboard, and momentarily display a cold temperature (stepping through stored programs, for

example), refrigeration

If

a temperature, higher than ambient,

will

and

is

closed and the unit

warm the rotor chamber accessories, while spinning (see Rapid Condition).

START,

without

the manual program, and that the instrument

is

switched on.

is

applied, as necessary,

will

not be activated.

by

to

cool the rotor

is

specified, the unit

wind friction

of

the rotors

If

the rotor chamber

will

not abort the spin. However, differs, at the start specified temperature. The the actual and set/programmed temperatures, until the temperatures come within S run should not continue

I.

Centra-CL3 Series Operation Manual

21

is

not at the specified temperature, it

if

the rotor chamber

of

a run,

by

more than S°C, from the

°C

display will switch between

0

C.

Press the

at

the actual temperature.

STOP

key,

two

if

the

The unit natural fanning action

angle rotors serves

is

not designed for use as a refrigerator. The

of

the rotating horizontal and fixed

to

maintain a uniform temperature

distribution, inside the chamber. At zero RPM, there is no

correlation between set and actual chamber temperatures.

Rapid Condition

Any frost should be removed. Allow it sponge or cloth. When the centrifuge is not

or

condensation, that forms in the rotor chamber,

to

melt, and remove it with a

in

use, turn it

off, or leave the cover open (to disable refrigeration).

is

When the chamber temperature (CL3R only)

above the

set temperature, Rapid Condition will run a rotor at 500

in

rpm, to increase air circulation

the chamber, to quickly

cool the chamber to the set point. When the chamber

is

temperature

will run the rotor at 2800 rpm, to warm the chamber

below the set temperature, Rapid Condition

to

the set temperature. When the temperature has been reached, three beeps will sound, and the rotor will brake to rest.

to

Some smaller rotors may not be able

to

higher temperature settings.

warm the chamber

To select this program, press the Program arrow keys, until

a C appears

in

the Program display. Select the desired

temperature, install a rotor, and press the START key.

Centra-CL3 Series Operation Manual

22

3.5

Diagnostic Messages and Error Codes

Diagnostics

Message

bAL

Lid

PFAII..

The beeper sounds

in

these situations:

• Two times on power up. of

• Three times at the end

a spin.

• Five times when a warning occurs

Diagnostic messages appear

in

place

of

the speed display,

the following cases:

Description

bAL

indicates an unbalanced rotor.

to

Open the cover,

Verify that a balanced load is installed. Inspect the rotor, and rearrange the

or

tubes,

Lid Close the cover,

PFAIL

resume the previous run. Press STOP, to erase this message. PFAII..

indicates a power failure. This message appears when the unit is turned back on, following the failure. The front panel will alternate between the PF AIL message and the remaining run time, or elapsed time,

mode.

add additional tubes, with fluid, to balance the rotor.

appears

erase this message.

if

you press the ST ART key, when the cover

to

erase this message.

if

power was interrupted during a run. Press START,

is

not closed.

if

in Hold

to

in

Error

Codes

Error Codes require factory-authorized maintenance. A

typical error means that the internal microprocessor has detected impennissible readings, the unit. Error messages appear

an

error code

power.

is

displayed, unplug and reconnect the unit to

If

the error code reappears, tell the service

or

a failure, elsewhere,

in

the speed display. When

personnel which message appeared, when you report the problem.

Centra-CL3 Series Operation Manual

23

in

Error Message

Err

1

OSPd

rEFR

FSAFE

COPF

COP

UndFI

ILLOP

dffi

Warnings HEAd,

case, the rotor brakes

Lid,

Description

No

Tachometer

Tachometer signals were not present during the run. The rotor coasts to a stop. Cover opening

Overs peed

Speed rotor. The rotor

Refrigeration Failure (Refrigerated only)

The unit displays this code, 45°C, at any time during the run.

Fail-safe Time

Independent circuitry, on the circuit board, has sensed a lack activity, from the control microprocessor. All power circuits (including motor, latch, solenoid, etc.) are disabled.

Cop Watchdog/OpCode

The microprocessor

program that controls the centrifuge. The stop.

COP

The microprocessor COP

a stop.

Undefined

The microprocessor was interrupted rotor will coast to a stop.

Op-Code

The rotor will coast to a stop

Wrong

The microprocessor discovered wrong direction during acceleration

during

PF

a spin:

AIL,

•

is

inhibited, after this error.

is

200 RPM above the maximum speed for the installed

will

brake to a stop.

if

the measured temperature exceeds

out

Trap

Error

has

sensed a

Watchdog

Not Enabled

is

Interrupt

Trap

Error

Direction

and

dffi

or

coasts to a stop, and the run ends.

of

Rotation

error messages can occur during a spin.

Jack

of

not enabled.

by

activity, from the

rotor

will coast to a

The rotor will coast

an undefined source.

of

rotation,

of

to

The

In this

Centra-CL3

Series

Operation

24

Manual

i

4 Applications

4.1

Introduction

This section describes the use accessories. rotor or accessory itself This section contains five reference sections:

• Speed and Force Tables

• Derating Table for Dense Samples

• Chemical Resistance Table

• Decontamination Table

• Nomograph

Caution: Do

rotor/accessory combination. Maximum rated speeds can

be found

Relative Centrifugal

speed varies with the rotor, and with the distance away (rotating radius) from the shaft rotation). The rotating radius

inside tip

Speed and Force Tables indicate the maximum speed and

RCF that the Centra-CL3/CL3R can achieve, with various

rotor/accessory combinations. The Derating Table specifies reductions specific gravity above 1.2.

More

detailed information

not

exceed maximum rated speed for each

in

Section 4.2 - Speed

Force

of

the tube, away from the centrifuge shaft. The

in

maximum RPM, when spinning samples with

of

specific rotors and

is

shipped with the

And

Force

(RCF or G-force) at a given

of

the centrifuge (center

is

measures to the furthest

Tables.

of

Centra-CL3 Series Operation Manual

25

Corrosive Solvents

Use

of

any tube above its rated RCF can cause tube

cracking.

To

avoid this, compare the G forces, specified

in

the Speed and Force Tables, with the ratings for the tubes

If

that you are using.

the tubes are not rated for the force that the centrifuge will apply, look up their reduced G force, and enter it on the control panel.

is

made

of

Your IEC centrifuge

materials designed to resist immediate attack from most laboratory chemicals. Prolonged exposure should be avoided, by immediately

removing the chemical from rotor accessories placed in the chamber are made

or

assembly. Rotors and

of

a variety

of materials, including aluminum and polypropylene. The Chemical Resistance Table shows the suitability

of

material with different classes

reagents.

of

each

Section 5

.2

describes how to clean and remove corrosion from the chamber, rotors, and accessories. Follow these instructions, and clean spills promptly, to minimize the effect

of

corrosive chemicals and to avoid expensive repairs.

Centra-CL3 Series Operation Manual

26

4.2 Speed and Force Tables

4 Place

Carrier

6560E

6561E

6562E

6563E Short

R t P k o

Rotor

(Use reauires 50968 taoered

No. Places

4 50 325/320**

or

Rotor

Number

ac ages

243

for

Use

of

Places

2

5 Falcon/Corning

9

Volume

Falcon/Corning

(100-125mm) 4000 3200 18

Blood Tubes

(75-lOOmm) 4000 3200 Blood Tubes

52151

215 4 Place Horizontal Swinging Bucket

shaft

of

Tube Volume (ml)

Trunnion/

Shield

or

Carrier (rpm) (xg) (cm)

only

50 ml

15

ml

Long

52152

with

Centra-CL3 and Centra-CL3R

Maximum RPM

4000 3200 18

4000 3200

52153

' ' '

adapter)

Max Max Max Speed

4100

RCF

2650

Maximum

52154

Radius O.D. x

Common

14.0 30 x 116

RCF

xg

Length

Tube

Radius

(cm)

18

(mm)

Size

'

\

4

8

12

16

20

4

**Order

50 350/323**

10

50

10

5-7

3 - 5

adapter 1106 and

310/356 4300

326/320 3600

355/356

366/1013 4100

36611018

366/369

3

1024

3800 2050 15.2 Falcon/Corning Conical

2750 13.2 17.2 x 113

2050 14.2 30 x 116

3600

4150

4600

2050

571

cushion, to spin

Centra-CL3 Series Operation Manual

1900

2400 12.8 16.5 x 107

2450 12.8 14.2 x

2625

500

15

ml Falcon/Corning Conical Tubes

27

13.2 17.2 x 112

11.1

10.6

16

x 75;

16

103

13

x 75;

13

12.6 x 83 10-12 x 75

I" x 3" Microscope Slide

x 100

Rt

ac age

P k o or

52212

Rotor

(Use

No. Places (ml)

221

reauires

of

6

6 Place Horizontal Swinging Bucket

50968

Tube Volume

10

15

tapered

Shield

shaft

adapter)

Max Max Speed (rpm) (xg) (cm)

356

303 4000 2600 14.6 17.2 x 119

4400 2800 13.0 17.2 x 102

RCF

Max Radius

0.0. x Length

Falcon Corning Conical Kimble 45168-125

Rotor 244 2-Place (Tray) Horizontal Microplate

(Use

reauires

of

No. Places (ml)

4

2

50968

tapered

Tube Volume Carrier

-

included#

49852 4000*

shaft

adapter)

Max Speed (rpm)

4000*

Max RCF (xg)

1800

Max

Radius (cm)

10.0

Width x Length

86 x 128

86 x 128 x 55mm

(mm)

(96

well) Microplates

Deep Plates

*Replacement carrier is IEC No. 49852 **

See derating table for 244 rotor

Rt

o or ac age

P k

Rotor

(Use

No.

Places

*With IEC 315 Conical Cushion

reauires

of

6

801

Tube Max Max Volume (ml)

58012

6-Place Fixed Angle 45°

50968

tapered

Shield

50 305

50

320 5200

shaft

adapter)

Speed RCF

(rpm)

4600

(xg)

2950

3300

Max Radius (cm)

12.1

10.8

0.0. x Length

30 x 133 Falcon

Coming

30

x 116

Corning 8300-50

Conical•

(mm)

Centra-CL3 Series Operation Manual

28

Rotor

(Use

reauires

804S 4-Place Fixed Angle 45°

50968

taoered

shaft

adapter)

with

323 Sealed Dome

Shields

No.

of

Places

4

*

Order

R t P k o

or

Rotor

(Use

reauires

No.

of

Places

12

Tube Volume (ml)

50

60

adapter 1106

ac age

Shield

323*

341

and

571 cushion, to spin

58092

Max Max Max Speed (rpm) (xg)

.

809

12-Place Fixed Angle 45°

50968

taoered

Tube Max Volume (ml) (rpm)

10

15

shaft

adapter)

Shield Speed

356

303 4800

RCF

7200 6675

2500 1000

15

ml

Falcon/Coming

Max RCF (xg) (cm)

5300

3200

2880

Radius

(cm)

11.5

14.3

Max

Radius

10.2

11.2

O.D. x

Falcon/Corning Conical

Coming

tubes

O.D. x

17.2 x 88

17.2xl02

Length

(mm)

12 15

R t P k o

or

Rotor

(Use

requires

No.

of

Places

24

4200

ac age

302

58153

815 24 Place Fixed

50968

Tube Volume (ml)

IO

15

taoered

Shield

shaft

adapter)

Max Speed (rpm) (xg)

356 4800

303

302 3800

4200

Angle

Max RCF

2860/2340

2360/1970

2100/1800

2480

33°

12.6

Max Radius (cm)

11.l/9.l

12/10

13/11

17.2 x 122

Falcon/Corning Conical

0.0.

x Length (mm)

17.2 x 88

17.2xl02

17.2 x 122

Falcon Coming Conical

Centra-CL3 Series Operation Manual

29

Rotor

p k

ac age 58162

Rotor

(Use reauires

No. Places

*With

Rotor

(Use reauires

No. Places

816 8-Place Fixed Angle 33°

Tube

of

Volume (ml)

8 50

IEC 315 cushion, order adapter 1106 and cushion 571

818

Tube

of

Volume (ml)

12

50968

tapered shaft adapter)

Shield

320 4600 2800 11.7 30 x 115

305 4300 2650 12.8

Max Speed RCF (rpm) (xg) (cm)

Max

12-Place Fixed Angle 35°

50968

tapered shaft adapteri

5

Adapter

Number

-

Max Speed RCF

(rpm)

8500

Max Max (xg) (cm)

6060 7.5

Max Radius

Radius

0.0.

x

Length

Corning

30 x 134

Falcon/Corning Conical*

to

spin Falcon/Corning tubes

8300-50

0.0.

12

x 80

x

Length

IEC

(mm)

2804, 2840

(mm)

12

Rotor

(Use reauires

No.

Of

Places

10

1.5 - 2

B-D/.5

.4/.25 5760

819 10-Place Fixed Angle 35°

Tube Volume (ml)

50968

10

5762

5761

tapered shaft adapter)

Adapter Number

-

8500 4680

8500

Max Max Speed (rpm)

8500

4770/3960

4680/3630

Max RCF (xg) (cm)

6060 7.6

Radius.

5.5

5.915.0

I

0.0.

16. l x 85

11x66

8

x66

6

x66

x

Length

(mm)

IEC 2046, 2067, 2801, 2850

Centra-CL3 Series Operation Manual

30

Rotor 836 6-Place Fixed Angle 30°

(Use

reci

uires

50968

taoered shaft adapter)

Max

(xg)

6060

No.

of

Places

6

Tube Volume (ml)

30

Adapter Number

-

Max Speed RCF (rpm)

8500

Max Radius

(cm)

7.5

O.D. x

25.8 x

Length

100

IEC

(mm)

2047,

2055,

2802

6

6 3

Rotor

(Use reQuires

No. Places

12

of

841

Tube Volume (ml)

1.5

12

15

5

5965

5966

5967

8500

5820

5650

5490

12-Place Fixed Angle 45°

50968

tapered shaft adapter)

- 2

B-D

.5

.4

Adapter Number

-

5763

5764

Max Max Max

Speed

(rpm)

8500

7.2

7.0

6.8

RCF Radius (xg) (cm)

4680

4770

3960

4680

16.1x109

12.1x112

10.9 x 95

5.8

5.9

4.9

5.8

O.D. x

Length

(mm)

11x39

B-D Microtainer Tubes

8 x

66

6 x

46

12

.25

5764

Centra-CL3 Series Operation Manual

8500

3630

31

4.5

6 x

46

Microtube Rotors

(Use requires 50036

Rotor Cat. No.

No.

hub

of

1.5 ml

adapter

Tubes and Tube Size

on

50968 tapered shaft

adapter)

Adapter

Cat. No Speed

Max

(RPM)

-

Max RCF

(xg)

6740

Radius

(cm)

8.35

8911

(Aerosol 24 x 0.5 ml PCR microtubes

Contained)

8512

8522

8532

24 x 0.6 ml

24 x 0 .4 ml microtubes 5764** 6740

24 x 0.25 ml microtubes 5764** 5860 24 x 1.5 ml 24 x 0.6 ml 24 x 0.5

24 x 0.4 24 x 0.25 ml microtubes 5764** 48 x 0.5 ml PCR microtubes

24 x 40

x 0.4

40 x 0.25

BID Microtainers™ 5763** 6830

ml

PCR microtubes 5763**

ml

microtubes 5764**

BID Microtainers ™

ml

microtubes

ml

microtubes

5763** 8500 6020

-

8500 6020

-

8500

-

8500

6740

6740 5860 6460 6060 6950

6790

6060

8.45

7.45

8.35

7.25

8.35

8.45

7.45

8.35

7.25

8.00t

7.50t

8.60t

8.40

7.50

40 x 0.8 ml (6x50 mm) glass

Microtainers® is a registered trademark

** Order 2 pks

t Outer row holds 24 tubes

:j:

Inner

1

IEC 891 Rotor Provides Aerosol Containment and has been tested for microbiological containment by PHLS-CAMR, Porton Down. Meets requirements Pathogen Final Rule: (Regulation 29 CFR Part 1910.1030. Complete Containment Cover, IEC 50525 Inner and IEC 36597 Outer rubber

2

IEC 851, 852, 853 rotors

of

adapters separately. IEC 5763 and 5764 are packaged 12/pk.

row holds 24 tubes

Centra-CL3 Series Operation Manual

of

Becton Dickinson

-

of

US

OSHA

with

0-ring

6870

32

8.50

Bloodborne IEC 50417 Aerosol seals.

245 Horizontal Rotor 4x250 ml

(Use reauires 50968 taoered shaft adapter)

of

Tubes x Tube Size

No.

40 x

1.5

ml

microtubes

40 x 3

ml

48

x 5

ml

5-7

ml

(13

x

75-lOOmm

28 x 10-15

16 x 15

12

4

4 x

4 x 175 ml (Falcon 2076) 61.0

ml

x 30

ml

x 50

ml

50

ml Falcon/Coming Conical

vacutainers)

ml

vacutainers 16.2

Falcon Conical

Max Tube Size

O.D.

mm

-

10.9

12.1

13.3

-

25.5

28.6

-

Ultrac

L.

mm

-

75

131

llO 7225

-

131

Adapter

7228

7226

7238

7230

7223

7231

- 7231

131

*

Max

RPM RCF

2750

2750 1450

2750 1450 17.2

2750 1450

2750 1475

2750 1450

2750 1450 17.2

2750 1475 17.6

Max Radius

(cm)

xg

1375

1450

16.2

17.2

17.6

17.2

t

l

I

4

x 200 ml (Nunc 376813) 61.0

4 x 225

4 x 250 ml (IEC 2502, 2504)

ml

(Falcon 2075) 61.0

* Also order Falcon 2090 cushions

**Also order IEC

5792 cushions

61.0

131

**

2750 1475 17.6

*

-

2750

2750 1500 17.8

1475 17.6

I

Centra-CL3 Series Operation Manual

33

268 Horizontal Rotor 4x250

(Use reauires 50968 taoered shaft adapter)

ml

No.

of

Tubes x Tube Size Max Tube Size

mm

O.D.

40 x 1.5 ml microtubes

40 x 3 ml

48 x 5 ml

40

x 7 ml

28 x 7-10 ml 16.2

12 x 15 ml Falcon Conical

12 x 30 ml

4 x 50 ml

4 x 50 ml Falcon Conical

4 x 75 ml (IEC 2059)

4 x 175 ml (Falcon 2074)

- -

10.9

12.1

13.3 106

-

25.5

28.6

- -

38.1

61.0

L.mm

75

106

-

106

110

112

125

Ultrac

Adapter

7228

7226

7236

7225

7234

7223

7222

7231

7221

*

Max Max Radius

RPM RCF

xg

3425 1800 13.7

3425

3425 1925

3425 1925 14.7

3425 1976

3425 1925 14.7

3425

3425 1925

3425 1925 14.7

3425 1975

1925

1925 14.7

(cm

14.7

15.1

14.7

15. l

4 x 250 ml (IEC 2261, 2051)

* Also order Falcon 2090 cushions

61.0

Centra-CL3 Series Operation Manual

125

34

-

3425

2000

15.3

958 Horizontal

(Use

reauires

50968

taDered

Rotor

shaft

6x50 ml

adapter)

No.

of

Tubes x

48 x 5 ml

36 x Shell vial

36 x 7

ml

30 x 5

ml

24 x 7

18

x 10 ml

10

24 x

18 x 10

6 x

12 x 50

ml 17.2

15

ml

15

ml 17.2

ml 30.0

Tube

Size

Max Tube Size TR.

O.D.

mm

13.6

17.7

12.6

14.2

16.5 110 366 1013

17.2

L.mm

92

77

88

98 366

106

103

112 355 356 2450

119 310 356 2950

134

120 326 320 2400

Ring

-

366 1018 2800

354 356 2400

310

Carrier or Shield

381 2600

379 2900

380 2600

369

303

Max

RPM

3200

2800

2700

Max RCF

xg

1125

1300

1125

1600

1375

1400

1025

1075

1575

1475

llOO

Radius

(cm)

15.0

13.7

15.0

14.0

15.7

15.9

16.1

17.6

16.9

6 x 50

ml

6 x Microscope Slides

Hemato-Kit

(Use

reouires

The 930 Hemato-Kit can convert the providing a simple, economical way packed in about ten minutes, at 7800 RPM/6200 xg. Kit includes numbered 24-place rotor for 1.75 x for installing rotor.

Order Cat.

75

No.

Rotor

1116

hublock

mm capillary tubes, 1505 gasket for tubes, cover, hublock adapter and wrench

An

930 Hemato-Kit and Cat.

930

adapter

accessory microcapillary tube reader available, to complete the system.

30.0

l"

on

50968

CL3

Series into a micro-hematocrit centrifuge,

to

perform occasional hematocrits. Blood cells are

No.

Centra-CL3 Series Operation Manual

124 325

3"

tapered

2201 Microcapillary Tube Reader.

shaft

35

-

adapter)

320

1025

2800

1800

1475

500

16.8

13.5

4.3

Derating Tables

Dense

Samples

The Speed and Force Tables list the maximum speed for each rotor/adapter combination for the CL3 Series. These speeds are guaranteed, for samples whose specific gravity not greater than:

• 1.2 for swinging bucket rotors

1.5

•

For denser samples, the maximum guaranteed speed

reduced ( derated) by a factor from the table below:

for fixed angle rotors

Specific

Gravity

1.2

1.3

1.4

1.5

1.6

1.7

1.8

1.9

2.0

2.1

2.2

2.3 .721

2.4

2.5

2.6

2.7

2.8

2.9

3.0 .632

Derating

Swingin2

1.000

Factor

Bucket

.960 .925 .894 .866 .839 .816 .794 .774 .755 .738

.707

.692

.678 .666 .654 .642

for:

Fixed

1.000

1.000 .967 .939 .912 .888 .866 .844 .825 .807 .790 .774 .758 .744 .731 .719 .707

is

An~le

is

Centra-CL3

Series Operation Manual

36

Derating Example:

used with samples with a specific gravity faster than

9,670 RPM. (10,000 x .967 = 9.670)

An

angle rotor, rated for 10,000 RPM,

of

1.6, cannot spin

Specific gravities greater than 3.0.

the formula:

You can use the same formula to compute derating factors

for specific gravities greater than

• S0 is the maximum specific gravity allowed before derating (1.2

•

Sa

is the actual specific gravity

Caution:

Higher speeds

wear on the centrifuge, and can cause

and under

Do

damage caused in this

warranty.

or

1.5, depending

not exceed the rated speed

or

specific gravities will impose unnecessary

manner

This table is based on

3.0.

on

the type

of

the sample.

rotor

are

of

or

specific gravity.

failure.

not covered

rotor.

Wear

Centra-CL3 Series Operation Manual

37

244 Rotor

The 244 rotor microplates. The weight equally distributed between the

is

designed for centrifugation

of

the loaded microplates must be

rotor's

of

multi-well

two carriers. The maximum rated speed for the 244 rotor, when each carriers is

4000

is

loaded with 280 grams (total load

rpm.

of

560 grams),

of

the

Caution: Loads greater than 280 grams (weight

of

the microplate and sample) must have maximum speed derated (according to the following table), to avoid

rotor failure.

Derating Table for 244 Rotor

Load per Carrier

( l!rams)

280

300 320 340 360 380 400 450 500 600

Greater than 600

Max. Speed

(rpm)

4000

3600 3300 3000 2800 2600 2400

1900 1400

800

do not use

Centra-CL3

Series

Operation Manual

38

4.4 Chemical Resistance

The

CL3 from most laboratory chemicals. A variety aluminum and polypropylene, comprise the rotors The Chemical Resistance table shows the suitability with different classes

Table

Series is made

of

materials that are designed to resist attack

of

materials, including

and

accessories.

of

each material,

of

reagents.

.

Acids, dilute Acids*, strong or cone. Alcohols, aliphatic Aldehydes G Bases

Esters Hydrocarbons, aliphatic Hydrocarbons, aromatic F Hydrocarbons, halogenated Ketones Oxidizing Agents, strong Salts

*For

or

weak

...

Ox1d1z1ng

PA - POL YALLOMER PCPE - POLYETHYLENE PP - POLYPROPYLENE PU

- POLYURETHANE NL - MODIFIED PHENYLENE OXIDE (NORYL) DN - ACETAL HOMOPOL YMER (DELRIN) CN - ACETAL COPOLYMER (CELCON) NN - NYLON

PS - POLYSTYRENE

Acids, see

POLYCARBONATE

PA

E E

E

G G

F

G

F

E

"Ox1d1zmg

Note: Chapter

5 describes how to clean

and

remove corrosion from chamber, rotors, and accessories. Follow the instructions, spills promptly,

minimize the effect

of

corrosive chemicals

to

avoid expensive repairs.

Plastic

PC

E E E G E F N F E G G F F N F N E

G E E F E E

F G G G G G G F N

N N

F G G E

N G F N N N F N N

E

. .

pp

PE

E

G G N

PU

NL

F N

E

E N G N

E

N

F

N N G E G N

G G N N

F F

E

Agents, strong" .

N N

N E E E E E E

DN

CN

NN

PS

Tl

N N N F

E N E E E E E F E

F

G

G E N

E

E E

E E E N E

N N N N N N

E E E E E

E E E E

E E E E E N N N

N

E E

E E E

E

N N N

E

N

E G G G E N N

E

Tl - TITANIUM SS

- STAINLESS STELL AL - ALUMINUM MB-

MANGANESE BRONZE MG - MAGNESIUM RR-RUBBER BN

- BUNA-N

VN

-VITON

PF

- PHENOLIC FIBER

Metal

SS

AL

MB

E E

E E N N E E

F

N N N N F E

F F

F

and

MG

E N

N

the

clean

and

Other

RR

BN

E E F E

N E

E

G

G N

E E

N N

E E E

VN

G G

F

N

PF

E

N

E E N

E E

E

Classification of Resistance

E=

Excellent G= Good F=

Fair

N= Not Recommended

Centra-CL3 Series Operation Manual

39

4.5 Decontamination Table

Compatible Processes

Sterilization

Mechanical

Autoclave•

Ethlene Oxide Gas

Dry Head 160°C@2Hrs. u u u u u

Chemical Ethanol

40% Formalin s s s

Methanol

2-Propanol s s s

5% Sodium Hypochlorite 3%

Hydrogen Peroxide s s s s s s M

100% Hydrogen Peroxide

5% Phenol Solution M u u

*For

PAPC-POLYCARBONATE PE - POLYETHYLENE PP - POLYPROPYLENE

PU NL - MODIFIED PHENYLENE OXIDE (NORYL) ON CN NNPS - POLYSTYRENE

Methods

...

Ox1d1zmg

POLYALLOMER

- POLYURETHANE

- ACETAL HOMOPOL YMER (OELRIN)

- ACETAL COPOLYMER (CELCON) NYLON

Acids,

For

Decontamination

Plastic

pp

PA

PC

PE

PU

s M u s M u s s s

s s s

s s s s u s s s u M

s

u s

s M s s M s s s u M s s s s

M s s s u s s

s

....

s s s s s s

s s s

s u u M M u M

see

. .

"Oxidizing Agents, strong".

ON

NL

s s s s s

u u

s

u s u

u

u u u

Tl - TITANIUM SS AL - ALUMINUM

MB

MG - MAGNESIUM

RRBN-

VN-VITON

PF PT - PAINTED SURFACES

Metal

Tl

CN

NN

PS

s s

u

u u u

s

u

s

s u s

s

- STAINLESS STELL

- MANGANESE BRONZE

RUBBER

BUNA-N

- PHENOLIC FIBER

SS

AL

s s s s

s

u s

s s

s

s s

s

s s M

M u

s s s s s

M M

s s

M M

Other

MB

MG

RR

BN

VN

s s s s

u

s

u u u u

s s

s

s s

s

M

u

s s

s

s s

u s

s

u s

s

s s

u u s u s

s u s s s

u u s

s

M M

*Autoclaving

12·c

2 ATM (15 PSIG)

....

S=SATIFACTORY M=MARGINAL

U=UNSATISFACTORY

u s s u

20

mnin.@

Household Bleach

PF

s M

s

s s s u

PT

s

u

s s

M M

Warning:

This chart describes the material compatibility specify the adequacy

for material compatibility during centrifugation.

of

sterilization. Refer

Centra-CL3 Series Operation Manual

to

40

of

various sterilization methods.

It

does not

section 4.4 - Chemical Resistance Table,

4.6

RCF

Nomograph

20

18

16

14

12

10

0

Q)

9

.c

~

8

fl)

::I

=o

7

(IS

a:

Cl

c

6

+:;

!!!

0

a:

5

4

3

50

45

40

35

30

25

~

!?

Q)

20

E

18

~

Using

VI

16

..2

"'O

Ill

a:

14

Cl

.5

ta

12

0

a:

•••

•• • centrifugal force is 1,000 xg (gravity).

·<10

9

8

7

6

the RCF Homograph

To

detennine force (ref), place a straightedge on the nomograph connecting the known speed (rpm) point Iha ref axis is the force.

For and the speed

II the force and Iha radius are known. Iha corresponding speed

the

relallve centrifugal

and

the known rotating radius. The

at

which the stral!fitedge intersects

example. if lhe rotating radius is 1 O

is

3,000 rpm, the relative

To Calculate RCF

RCF

.,

.00001118 x r x N'

RCF • relative centrifugal force (gravhies)

r • rotating racius (centimeters)

z

rotaUng

N

speed

can

be determined.

(rev.

per

cm

min. or rpm)

20,000

15,000

30,000 20,000

10,000

5,000

Ci)

j!

~

2,000

El

Q)

......

············

~--·~:~···

····~

~

;;:

'E

Q)

(.)

Q)

.::!:

1i5

Qi

a:

500

200

.~1

100

50

20

10

10,000

6,000

4,000

......

3,000

2,000

1,000

500

-···

';1~

.s

~

c:

E

....

Q)

Q.

(/J

c:

.2

"5

0

>

Q)

a:

I

'"O

Q) Q)

Q.

en

r~•

1-·

':,t

..

'-~

5

0

200

Centra-CL3 Series Operation Manual

41

5 Maintenance

5.1

5.2

Introduction

Care

and

Cleaning

This chapter explains how to keep your unit in good

operating order. decontaminating, and storing. This chapter, also, covers the cover interlock bypass.

See the end warrantees.

Keep your centrifuge clean, to ensure good operation, and to extend its life.

Clean the sample chamber, rotor, and lid, at the end work day, and immediately after any spill. chamber, use a damp sponge, warm water, and a mild liquid detergent, suitable for washing dishes by hand, such as Ivory® liquid. Do not use caustic detergents, that contain chlorine ions. These attack metals.

It

includes instructions for cleaning,

of

the chapter, for information on service and

To

clean the

or

detergents

of

each

Remove stubborn stains with a plastic scrub pad. Do not use steel wool, wire brushes, abrasives, create corrosion sites. Never pour water directly into the rotor chamber.

Centra-CL3 Series Operation Manual

43

or

sandpaper. They

Scrub the rotor's tube cavities with a stiff test tube brush

that has end bristles and a non-metallic tip.

Dry each part,

after cleaning, with a clean, absorbent towel.

If

glass breakage occurs, remove

If

immediately. cushions. Particles

breakage recurs, replace

of

broken glass embed in the plastic

all

broken pieces

all

adapters and

or rubber accessories. Glass particles can come in contact with new glass tubes, creating pressure points that may result breakage recurring. Glass particles,

in

the chamber, grind

in

to

a fine gray dust, during centrifugation. This dust can coat

of

the inside

the centrifuge.

Corrosion

IEC manufactures and finishes rotors and

structural accessories corrosion. To maximize the inspect the rotor cavities for corrosion, especially

to

give maximum resistance to

life

of

the unit, continually

if

you use chloride ion solutions, such as sodium chloride (saline), and sodium hypochlorite (household bleach), because these solutions attack most metals. Clean the rotor, rotor chamber, and accessories (particularly the sample compartments and bucket cups) thoroughly, after each exposure. Inspect

all

surfaces, under bright light, for corrosion. Be aware that small crevices grow deeper, eventually resulting in system failure.

Replace the shaft, locking nut, rotor, or accessories,

if

they

become corroded, cracked, deformed, or gouged.

Centra-CL3

Series

Operation

44

Manual

I'

t·

'I

If

you see any corrosion, remove it immediately, using the

following procedure:

1.

Follow the cleaning procedure, at the start

of

this

section.

2.

Soak the product

in

mild hand dish washing detergent, and scrub the product thoroughly with a stiff test tube brush. The brush should have end bristles and a nonmetallic tip.

3.

Soak the product, again, in clear warm water, for a

minimum

of

an

hour.

4. Rinse the product in warm water, then in distilled water.

5.

Dry the product, thoroughly, with a clean, absorbent

cloth.

Storage

Decontamination

Caution:

discontinue use

If

this procedure does not remove the corrosion,

of

the product.

Store parts on a soft surface, to avoid damage. Rotors and other parts should be clean and dry. Store them open to the

air,

not

in

a plastic bag, so that any residual moisture evaporates. Face the parts upward, to avoid moisture retention

If

tube breakage occurs, releasing toxic, infectious,

in

the cavities.

pathogenic, or radioactive material into the unit,

decontaminate the chamber.

Rotors have sealed containers, that provide aerosol

containment and, if used as directed, keep spillage confined.

If

breakage occurs, it may be sufficient

to

only

decontaminate the sealed carriers.

The Decontamination Table,

of

sensitivity

various materials to common sterilization

procedures. When using a 1-to-10 dilution

in

Chapter

4,

lists the

of

household

bleach (sodium hypochlorite), to decontaminate metal rotors

or accessories, follow decontamination by the corrosion cleaning procedure (See 5.2), since chloride ions attack most metals.

Centra-CL3 Series Operation Manual

45

Always decontaminate for the minimum recommended time.

If

you observe corrosion, remove it, as described earlier;

of

discontinue use

the method; and use an alternate

decontamination procedure.

Polypropylene sealed carriers can be autoclaved. Remove

any sample tubes, before autoclaving, unless they are

full

of

completely other tube closures, before autoclaving,

sample. Remove caps, stoppers, and

to

keep the tubes from collapsing under pressure. Autoclave the rotor and accessories at

° C @

15

psig for 20 minutes. Do not

121 stack polypropylene rotors during this process. After cooling, perform a normal cleaning operation, as described above.

1

Repeated autoclaving seriously degrades the performance polycarbonate sealing covers.

5.3 Cover Interlock Bypass

The cover remove samples from the unit, before power the cover interlock bypass, after the rotor has come to a

stop.

To bypass the cover interlock:

1.

Unplug the centrifuge.

2.

Locate the hidden plastic plug, underneath the ledge

below the IEC CL3 logo.

3.

Use a screwdriver to remove the plug.

4.

Pull the attached cord, to release the cover interlock.

5.

Replace the plug

Do

not perform this operation routinely. The centrifuge's

cover interlock provides operator safety.

cover

to

stopped.

will

remain locked,

in

the hole.

if

power fails.

If

you need

is

restored, use

It

allows the

be opened promptly, whenever rotation has

of

to

Centra-CL3

Series

Operation Manual

46

. 5.4 Condition of Returned Equipment

5.5 Warranty

Contact IEC, or your dealer, and obtain a return authorization (RGA), before returning equipment The RGA paperwork includes a Certificate Decontamination for you to sign.

It

indicates that you have

of

goods

to

IEC.

performed the proper steps for decontaminating your unit.

All

Warning: of

radioactivity,· and free

pathogenic,

IEC refuses certificate

returned units must be decontaminated,

or

toxic materials.

all

return equipment shipments, until the signed

is

received.

of

hazardous, ·infectious,

free

You must prepay transportation to the service depot.

of

IEC wants you to be satisfied with the quality

your CL3 Series centrifuge. We warranty your IEC centrifuge for one year, and IEC rotors for seven years. We replace from the date

any

of

these products that

of

its delivery, due to defects

fail,

will

repair

or

within this period,

in

material and workmanship, and we will ship you the repaired product or its replacement at our expense. You must use IECapproved rotors and accessories, and genuine IEC spare

parts. This warranty does not apply to any instrument that has

been repaired without authorization or abused.

THE FOREGOING OBLIGATIONS ARE IN LIEU OTHER OBLIGATIONS NEGLIGENCE, AND MERCHANTABILITY OR OTHERWISE, EXPRESSED IMPLIED OUR ENTIRE AND EXCLUSIVE LIABILITY, AND EXCLUSIVE REMEDY, CONNECTION WITH THE SALE OR FURNISHING GOODS USE, INSTALLATION,

EVENT BE CONSEQUENTIAL DAMAGES WHATSOEVER AND LIABILITY UNDER NO CIRCUMSTANCES

THE PURCHASE PRICE FOR THE GOODS FOR WHICH

LIABILITY

Centra-CL3 Series Operation Manual

IN

FACT OR

OR

PARTS, THEIR DESIGN,

LIABLE

IS

CLAIMED.

47

AND

LIABILITIES INCLUDING

ALL

WARRANTIES, OF

BYLAW.

FOR

ANY CLAIM OR

OR

OPERATION. IEC

FOR

ANY

SPECIAL OR

THE FOREGOING

SUITABILITY

OF

BUYER'S

DAMAGES

WILL

EXCEED

ALL

OR

STATES

OF

FOR

IN

OUR

IN

NO

6 Specifications

I I

Maximum Speed:

Maximum

Maximum Sample Volume:

Operator Controls:

Chamber Temperature*: Speed:

Spin Duration:

Force:

Number

of

Tubes:

8,500

RPM

(841 fixed angle rotor)

4,000

RPM

(243 swinging bucket rotor)

6,200 xg (930 fixed angle rotor) 3,200 xg (243 swinging bucket rotor)

48 x

5 ml sealed (243 rotor)

12

x 25 ml Universal sealed (243 rotor)

20 x

15

ml Falcon/Corning sealed (243 rotor)

8

x 50

ml

Falcon/Corning sealed (243 rotor)

4

x 250 ml (243 rotor)

4

x Microplates/2 x Deep plates (244 rotor)

1 liter (243 rotor)

by 1 C

0

15

seconds

-9° C to 40° 0 - 8,500 RPM, by 100 RPM

0:01 through 0:59, by 1 second 1 :00 through 4:45, by 5 through 99 min., by 1 min. Momentary operation mode Hold mode (indefinite duration) At-Start timing mode At-Speed timing mode

C,

Repeatability:

Temperature*: Rotation:

Motor:

Refrigerant*:

0

± 1 C

in the range from +4° C to +22° C

10

RPM

to

Brushless

HP-80, CFC-free

Centra-CL3 Series Operation Manual

49

DC

Power Requirements

Voltage:

\ -

.)..

3750 - 120V/60

Hz

3751 - 220-240V/50/60 Hz 3755 - 120V/60 3756 - 220-240V/50 3757 - 220-240V/60

Hz

Hz Hz

Full specification compliance is guaranteed for up to ±5% line voltage specified. Function guaranteed up to ±10%

ofline

voltage

Operating Current:

Heat Output:

(max)

CL3: CL3R:

Dimensions (CL3R)

Sample Loading Height: Cover Closed Height: Cover Open Height: Width: Depth: Weight:

(shipping weight) (net weight)

~~-..#..-.

Dimensions (CL3)

Sample Loading Height: Cover Closed Height: Cover Open Height:

Width:

Depth: Weight:

(shipping weight) (net weight)

•,,•·,.

3750 - 5.5 AMPS 3751 - 3 AMPS 3755 -

10 AMPS

3756 - 5 AMPS 3757 - 5 AMPS

1500 BTU/Hr

2700 BTU/Hr

32 cm

11::

" in) .

':

cm

i , -

.~·

in)

~cm·.·~:

'o

en~

~~

c1r.

68 kg

C •

(:'

(l);:

.:1)

:=.

in)

~

-~i)

;;JS)

46 kg (lOOibs)

32 cm (12.5 in) 37

cm

(14.5 in)

84 cm (33 in)

49

cm (19.25 in)

53

cm (21 in)

91

kg (200 lbs)

68 kg (150 lbs)

Sound Level:

60 db(A) max.

Specifications Subject To Change Without Notice

* CLJR Only

Centra-CL3 Series Operation Manual

50