Idexx SNAP 4Dx Plus Quick Reference Manual

SNAP® Test Quick Reference Guide

PageSNAP Test Quick Reference Guide Return to table of contents 2

Supported species.

8

min

Read time in minutes.

Store at room temperature or in refrigerator.

Store in refrigerator.

Table of contents

Key

SNAP test kits at a glance............................................................................3

SNAP Pro® Analyzer ....................................................................................4

SNAP® 4Dx® Plus Test .................................................................................5

SNAP® Heartworm RT Test ..........................................................................6

SNAP® Feline Triple® Test .............................................................................7

SNAP® FIV/FeLV Combo Test .......................................................................8

SNAP® cPL™ Test .......................................................................................9

SNAP® fPL™ Test ......................................................................................10

SNAP® Parvo Test .....................................................................................11

SNAP® Giardia Test ...................................................................................12

SNAP® Feline proBNP Test ......................................................................... 13

SNAP® Lepto Test......................................................................................14

SNAP® FeLV Antigen Test ...........................................................................15

SNAP® Foal IgG Test .................................................................................. 16

SNAP test cycling and shipping guidelines .................................................17

ALL components MUST be at room temperature before the test is run.

PageSNAP Test Quick Reference Guide Return to table of contents 3

SNAP test kits at a glance

Supported sample types

Storage*

Refrigerated = 2°C–8°C

Room temperature = 2°C–25°C

Sample size

Drops of

solution

Read

time

(minutes)

Test name

†

Fecal

Anticoagulated

whole blood

Plasma Serum

SNAP® 4Dx®

Plus Test

• • •

Refrigerated until expiration, or

room temperature for 90 days

3 drops 4 8

SNAP

®

Heartworm

RT Test

• • •

Room temperature until expiration 3 drops 4 8

SNAP

®

Feline

Triple® Test

• • •

Refrigerated until expiration

3 drops 4 10

SNAP

®

FIV/FeLV

Combo Test

• • •

3 drops 4 10

SNAP

®

cPL™ Test

•

3 drops 4 10

SNAP

®

fPL™ Test

•

3 drops 4 10

SNAP

®

Parvo Test

•

Room temperature until expiration

Coat entire swab tip

with a thin layer of

fecal material.

5 8

SNAP

®

Giardia Test

•

Refrigerated until expiration

Coat entire swab tip

with a thin layer of

fecal material.

5 8

SNAP

®

Feline

proBNP Test

• •

3 drops 5 10

SNAP

®

Lepto Test

•

3 drops 4 10

SNAP

®

FeLV

Antigen Test

• • •

3 drops 4 10

SNAP

®

Foal

IgG Test

• • •

1 drop

Entire bottle,

or until well

is full

7

*Refer to package insert for complete storage requirements.
†Before beginning any test, refer to the package insert for complete instructions.

PageSNAP Test Quick Reference Guide Return to table of contents 4

SNAP Pro® Analyzer

*Before beginning any test, refer to the package insert for complete instructions.

IMPORTANT: Prepare the SNAP test, but do not
activate the test.*

When prompted by the SNAP Pro, gently slide the
SNAP test into the port at the bottom of the analyzer
(well end first) until it clicks into position.

Solid green

All results are negative.

Flashing blue

At least one result is positive.

Flashing yellow

Visual confirmation needed.

(See operator’s guide for more information).

Wrong Right

When analysis is complete, results are shown on the results screen and are saved on the analyzer
automatically. The color of the status light indicates the results of the run:

For more information or to order this product, visit idexx.com/prohelp.

Icon Description

Wireless (Wi-Fi) signal status
(5 gray bars = best signal; 5 red bars = no signal)

Lost Wi-Fi communication

Connected and communicating with the
IDEXX VetLab

®

Station via a wired connection

(red icon indicates the analyzer is off-line)

Lost Ethernet connection

Battery charging

Icon Description

Software upgrade is available
(SmartService

®

Solutions customers only)

Warning notification (gray icon indicates the
warning has been viewed and is unresolved)

Error notification (gray icon indicates the error
has been viewed and is unresolved)

Lost communication with IDEXX VetLab Station

Interpreting SNAP Pro icons

PageSNAP Test Quick Reference Guide Return to table of contents 5

SNAP® 4Dx® Plus Test

Test procedure

†

25°C

*

2°C

*Tests expire after 90 days at room temperature. If not stored at room temperature, tests can be refrigerated
until expiration date. Refer to package insert for complete storage requirements.
†Before beginning any test, refer to the package insert for complete instructions.

§Anticoagulated whole blood (e.g., EDTA, heparin).

Screen dogs annually for six vector-borne diseases.

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP 4Dx
Plus device.

5. Read the result at

8 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

8

min

Can be stored at room

temperature for 90 days.*

Positive control

E. canis/E. ewingii Ab

D. immitis Ag

B. burgdorferi Ab

A. phagocytophilum/
A. platys Ab

Whole blood

§

, serum,

or plasma sample

For more information or to order this test, visit idexx.com/snap4dxplus.

PageSNAP Test Quick Reference Guide Return to table of contents 6

SNAP® Heartworm RT Test

Test procedure*

25°C

2°C

Screen annually for heartworm.

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube
into the sample
well of a SNAP
Heartworm RT
device.

5. Read the result at

8 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

8

min

Positive control

High antigen indicator

Negative control

Whole blood

†

, serum,

or plasma sample

For more information or to order this test, visit idexx.com/heartworm.

Low antigen indicator

*Before beginning any test, refer to the package insert for complete instructions.
†Anticoagulated whole blood (e.g., EDTA, heparin)

PageSNAP Test Quick Reference Guide Return to table of contents 7

SNAP® Feline Triple® Test

Test procedure*

8°C

2°C

Test all new-to-household, sick, and at-risk cats, as well as cats with respiratory
or vomiting symptoms, for feline immunodeficiency virus (FIV), feline leukemia
virus (FeLV), and feline heartworm.

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP Feline
Triple device.

5. Read the result at

10 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

10

min

*Before beginning any test, refer to the package insert for complete instructions.
†Anticoagulated whole blood (e.g., EDTA, heparin)

Positive control

FIV Ab

Feline heartworm Ag

FeLV Ag

Whole blood

†

, serum,

or plasma sample

For more information or to order this test, visit idexx.com/snapfelinetriple.

PageSNAP Test Quick Reference Guide Return to table of contents 8

SNAP® FIV/FeLV Combo Test

Test procedure*

8°C

2°C

Test all new-to-household, at-risk, and symptomatic cats for feline
immunodeficiency virus (FIV) and feline leukemia virus (FeLV).

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP
FIV/FeLV Combo
device.

5. Read the result at

10 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

10

min

*Before beginning any test, refer to the package insert for complete instructions.
†Anticoagulated whole blood (e.g., EDTA, heparin)

Positive control

FIV Ab

Negative control

FeLV Ag

Whole blood

†

, serum,

or plasma sample

For more information or to order this test, visit idexx.com/snapcombo.

PageSNAP Test Quick Reference Guide Return to table of contents 9

SNAP® cPL™ Test

Test procedure*

8°C

2°C

Test dogs that present with vomiting, anorexia, and/or abdominal pain.

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP cPL
device.

5. Read the result at

10 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

10

min

*Before beginning any test, refer to the package insert for complete instructions.

For more information or to order this test, visit idexx.com/snapcpl.

Normal

Sample spot is lighter than reference spot.

Reference Pancreas-specific

lipase

Abnormal

Sample spot is the same color or
darker than reference spot.

Serum
sample

PageSNAP Test Quick Reference Guide Return to table of contents 10

SNAP® fPL™ Test

Test procedure*

8°C

2°C

Test cats that present with lethargy, decreased appetite, dehydration,
weight loss, vomiting, and/or diarrhea.

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP fPL
device.

5. Read the result at

10 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

10

min

*Before beginning any test, refer to the package insert for complete instructions.

For more information or to order this test, visit idexx.com/snapfpl.

Normal

Sample spot is lighter than reference spot.

Reference Pancreas-specific

lipase

Abnormal

Sample spot is the same color or
darker than reference spot.

Serum
sample

PageSNAP Test Quick Reference Guide Return to table of contents 11

SNAP® Parvo Test

25°C

2°C

Test dogs that present with sudden onset of lethargy, vomiting, fever, and diarrhea.

1. Swab sample‡ and
place swab tip into
tube. Bend bulb
to break seal and
release conjugate.

2. Squeeze and
release bulb
3 times to mix
sample and
conjugate.

3. Squeeze bulb to

dispense 5 drops
into the sample
well of a SNAP
Parvo device.

5. Read the result at

8 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

8

min

Test procedure

†

*Refer to package insert for complete storage requirements.
†Before beginning any test, refer to the package insert for complete instructions.
‡The collection swab is not intended to be used rectally.

Can be stored at

room temperature.*

Positive control

Negative control

Fecal sample

For more information or to order this test, visit idexx.com/parvo.

Parvovirus Ag

PageSNAP Test Quick Reference Guide Return to table of contents 12

SNAP® Giardia Te st

Test procedure*

*Before beginning any test, refer to the package insert for complete instructions.
†The collection swab is not intended to be used rectally.

Test dogs and cats that present with diarrhea.

1. Swab sample† and
place swab tip into
tube. Bend bulb
to break seal and
release conjugate.

2. Squeeze and
release bulb
3 times to mix
sample and
conjugate.

3. Squeeze bulb
to dispense
5 drops into the
sample well of
a SNAP Giardia
device.

5. Read the result at

8 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

8

min

8°C

2°C

Positive control

Negative control

Fecal sample

For more information or to order this test, visit idexx.com/giardia.

Giardia Ag

PageSNAP Test Quick Reference Guide Return to table of contents 13

SNAP® Feline proBNP Test

Test procedure*

8°C

2°C

Test cats at risk for cardiac disease (murmur, arrhythmia, respiratory signs, prior
to anesthesia, breed, history, age).

1. Using the provided
pipette, dispense
3 drops of sample
and 5 drops of
green conjugate to
the sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP Feline
proBNP device.

5. Read the result at

10 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

10

min

*Before beginning any test, refer to the package insert for complete instructions.

For more information or to order this test, visit idexx.com/snapprobnp.

Normal

Sample spot is lighter than reference spot.

Reference NT-proBNP

Abnormal

Sample spot is the same color or
darker than reference spot.

Serum or EDTA
plasma sample

PageSNAP Test Quick Reference Guide Return to table of contents 14

SNAP® Lepto Test

Test procedure*

8°C

2°C

Test dogs when leptospirosis is suspected.

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP Lepto
device.

5. Read the result at

10 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

*Before beginning any test, refer to the package insert for complete instructions.

10

min

For more information or to order this test, visit idexx.com/snaplepto.

Positive control

Leptospira Ab

Serum sample

PageSNAP Test Quick Reference Guide Return to table of contents 15

SNAP® FeLV Antigen Test

Test procedure*

1. Using the provided
pipette, dispense
3 drops of sample
and 4 drops of
conjugate to the
sample tube.

2. Gently invert 3–5
times to mix.

3. Pour the entire
contents of the
sample tube into
the sample well
of a SNAP FeLV
Antigen device.

5. Read the result at

10 minutes.

Activation
circle

4. Use the SNAP Pro® Analyzer
to automatically activate the
SNAP test and interpret the
result.

OR

When color first appears in
the activation circle, press
firmly to activate. You will
hear a distinct “snap.”

10

min

Test cats for feline leukemia virus (FeLV).

8°C

2°C

Positive control

FeLV Ag

Negative control

Whole blood

†

, serum,

or plasma sample

For more information or to order this test, visit idexx.com/snapfelv.

*Before beginning any test, refer to the package insert for complete instructions.
†Anticoagulated whole blood (e.g., EDTA, heparin)

PageSNAP Test Quick Reference Guide Return to table of contents 16

SNAP® Foal IgG Test

Test procedure*

*Before beginning any test, refer to the package insert for complete instructions.
†Anticoagulated whole blood (e.g., EDTA, heparin), serum, or plasma sample.

Test foals for immunoglobulin G (IgG) levels.

1. Dilute sample.†
Mix thoroughly
and dispose of
the first 5–10
drops from the
bottle.

2. Apply 1 drop of sample

over sample spot.

3. Pour the
entire bottle of
conjugate into
the sample well.

5. Read the result at

7 minutes.

4. When color first appears

in the activation circle,
press firmly to activate.
You will hear a distinct
“snap.”

Activation
circle

7

min

8°C

2°C

Sample spot

400 mg/dL
calibrator spot

Sample spot

800 mg/dL
calibrator spot

Whole blood

†

,
serum, or
plasma sample

For more information or to order this test, visit idexx.com/foal.

≤400 mg/dL IgG

Color intensity of sample spot is lighter than
or equal to the 400 mg/dL calibrator spot.

400–800 mg/dL IgG

Color intensity of sample spot is darker than
the 400 mg/dL calibrator spot but lighter than
or equal to the 800 mg/dL calibrator spot.

>800 mg/dL IgG

Color intensity of sample spot is darker than
the 800 mg/dL spot.

PageSNAP Test Quick Reference Guide Return to table of contents 17

SNAP test cycling and shipping guidelines

*These tests can be stored at room temperature, which can eliminate the need for cycling—refer to the SNAP test package insert or idexx.com for details.

Cycling is the optional process of removing the product from the refrigerator, allowing it to
equilibrate to room temperature for 30 minutes–8 hours, and then returning the product to the
refrigerator.

IMPORTANT: When cycling, keep the SNAP tests in their foil package and the conjugate bottled.
To learn more about your SNAP test, including appropriate storage temperatures and how to
achieve optimal performance, refer to the SNAP test package insert.

SNAP

®

4Dx® Plus Test*
SNAP® cPL™ Test
SNAP® Feline proBNP Test
SNAP® Feline Triple® Test
SNAP® FeLV Antigen Test
SNAP® FIV/FeLV Combo Test

SNAP® Foal IgG Test
SNAP® fPL™ Test
SNAP® Giardia Test
SNAP® Heartworm RT Test*
SNAP® Lepto Test
SNAP® Parvo Test*

Each SNAP test and conjugate bottle listed below can be cycled up to 30 times.

For more information on each test, visit idexx.com/snap.
If you have any questions, contact IDEXX Customer Support at 1-800-248-2483.

As part of our development process, we perform stability studies, including heat stress
testing, to ensure that our tests can withstand reasonable shipping stress.

In our heat stress testing process, we measure the performance of test components over time
after exposure to various temperatures. On this basis, we ship our diagnostic tests as follows:

• Tests shipped at ambient temperature: SNAP 4Dx Plus, SNAP Heartworm RT,

SNAP FIV/FeLV Combo, SNAP Giardia, SNAP Parvo, SNAP cPL, and SNAP Foal IgG. These
tests must be received within 48 hours of shipment. Store tests appropriately immediately
upon receipt.

• Tests shipped on ice (48 hours): SNAP fPL and SNAP Feline proBNP. These tests must

arrive within 48 hours of shipment. Store tests appropriately immediately upon receipt.

• Tests shipped on ice (24 hours): SNAP Feline Triple, SNAP FeLV Antigen, and

SNAP Lepto. These tests must arrive cold within 24 hours of shipment. Store tests
appropriately immediately upon receipt.

© 2019 IDEXX Laboratories, Inc. All rights reserved. • 06-0010825-00
All ®/TM marks are owned by IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

R

E

F

E

R

E

N

C

E

L

A

B

Q

U

A

L

I

T

Y

I

N

T

H

E

P

A

L

M

O

F

Y

O

U

R

H

A

N

D

SNAP

®