user manual
Hoefer
SQ33 Sequencer
Includes Fan Sensor Kit
um SQ33-IM/Rev.B0/03-13
Page finder
Important Information ...........................................ii
Waste Electrical and
Electronic Equipment (WEEE) ............................... ix
1. SQ33 Function and Description .........................1
2. SQ33 Specifications ..........................................2
3. SQ33 Operating Instructions .............................. 4
4. Installation and Operation
of the Fan Sensor Kit .......................................11
5. SQ33 Care and Maintenance ............................13
6. Customer Service Information ........................... 14
7. Disposal .........................................................15
8. Warranty .........................................................16
9. Ordering Information ........................................18
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•
Important Information –
English
SQ33:
• If this equipment is used in a manner not specified by
Hoefer, Inc. the protection provided by the equipment may
be impaired.
• This instrument is designed for indoor laboratory use only.
• Only accessories and parts approved or supplied by Hoefer,
Inc. may be used for operating, maintaining, and ser vicing
this product.
• Only use a power supply that is CE mar ked or safety certified
by a nationally recognized testing laboratory.
• The safety lid must be in place before connecting the power
supply leads to a power supply.
• Turn all power supply controls off and disconnect the power
leads before removing the safety lid.
• Circulate only water or 50/50 water/ethylene glycol through
the heat exchanger if so equipped. Do not connect the heat
exchanger to a water tap or any coolant source where the
water pressure is unregulated.
• Never introduce antifreeze or any organic solvent into any
part of the instrument. Organic solvents will cause irreparable damage to the unit!
• Do not operate with buffer temperatures above the maximum specified technical specifications. Overheating will
cause irreparable damage to the unit!
Fan Sensor Kit:
• Warning! Because this instrument can develop sufficient
voltage and current to produce a lethal shock , care must be
exercised in its operation.
• This instrument is designed in accordance with the
EN61010-1:2001 electrical safety standard. Nevertheless, it
should be used only by properly trained operators. Read
this entire manual before using the instrument and use only
according to the instructions.
• The instrument must always be used with the ear th lead of
the power cord correctly grounded to earth at the mains
outlet.
• Use only undamaged electrical wire and equipment specific
for the voltages you will use. All equipment connected to
high voltage should be in accordance with EN61010-1:2001.
• Keep the instrument as dry and clean as possible. Wipe
regularly with a soft, damp cloth. Let the instrument dry
completely before use.
• Do not operate the instrument in extreme humidity (above
80%). Avoid condensation by letting the unit equilibrate to
ambient temperature when taking the instrument from a
colder to a warmer environment.
• To permit sufficient cooling, ensure that the vents of the
instrument are not covered.
Duležité informace – Czech
SQ33:
• Pokud toto zařízení používat způsobem, který není
specifikován Hoefera, Inc ochrana poskytovaná zařízením
může být snížena.
• Tento přístroj je určen pro vnitřní laboratorní použití.
• Pouze příslušenství a díly schválené nebo dodané Hoefera,
Inc mohou být použity pro provoz, údržbu a servis tohoto
produktu.
• Používejte pouze napájecí zdroj, který je označen značkou
CE nebo bezpečnost certifikována celostátně uznávanou
testovací laboratoří.
• Bezpečnostní víko musí být na místě před připojením
napájení vede k napájení.
• Zapněte všechny napájení kontroluje a odpojte napájecí
vodiče před vyjmutím bezpečnostní víko.
• Uváděny do oběhu pouze vodu nebo 50/50 vody/ethylen
glykolu přes výměník tepla, pokud tak vybaven. Nepř ipojujte
výměníku tepla na vodovodní kohoutek nebo chladicí zdroj,
kde je tlak vody neregulované.
• Nikdy zavést nemrznoucí nebo jakákoli organická
rozpouštědla do jakékoli části přístroje. Organická
rozpouštědla způsobí nenapravitelné škody na zařízení!
• Nepracujte s nárazníkových teplotách nad maximální
uvedených technických specifikací. Přehřátí může způsobit
nenapravitelné škody na zařízení!
Fan Sensor Kit:
• Varování! Vzhledem k tomu, tento nástroj se může vyvinout
dostatečné napětí a proudu k výrobě smr tící šok, péče musí
být vykonávána v provozu.
• Tento nástroj je navržen v souladu s EN61010-1: 2001 Elektrická bezpečnost standardní. Nicméně, mělo by být použito
pouze řádně vyškoleni operátorů. Přečtěte si celou tuto
příručku před použitím přístroje a používat pouze v souladu
s pokyny.
• Přístroj musí být vždy používán s země vedením napájecího
kabelu řádně uzemněné na zem na zásuvky.
• Používejte pouze nepoškozený elektrické dráty a zařízení
specifických pro napětí, které budete používat. Všechna
zařízení připojeno k vysokým napětím by měla být v souladu
s EN61010-1: 2001.
• Udržujte přístroj jako suchý a čistý, jak je to možné. O třete
pravidelně měkkým, vlhkým hadříkem. Nechte přístroj dokonale vyschnout před použitím.
• Nepoužívejte přístroj v extrémní vlhkostí (nad 80%). Zabránit
kondenzaci par nechat přístroj vytemperovat na pokojovou
teplotu při vyjmutí přístroje z chladnějšího do teplejšího
prostředí.
• Chcete-li povolit dostatečné chlazení, zajistit, aby otvory pro
přístroje se nevztahuje.
pii
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Vigtig information – Danish
SQ33:
• Hvis dette udstyr bruges på en måde, der ikke er specificeret af Hoefer, Inc. den beskyttelse, som udstyret kan være
værdiforringet.
• Dette instrument er beregnet til indendørs laboratorium
brug.
• Kun tilbehør og dele godkendt eller leveret af Hoefer, Inc.
kan anvendes til drift, vedligeholdelse og service af dette
produkt.
• Brug kun en strømforsyning, der er CE-mærket eller sik kerhedscertificeret med et statsanerkendt prøvningslaboratorium.
• Sikkerheden Låget skal være på plads, før du slutter strømforsyningen fører til en strømforsyning.
• Drej alle strømforsyningen kontrollerer og frakobl strømforsyningen, før du fjerner sikkerheds låg.
• Cirkulere kun vand eller 50/50 vand/ethylenglycol gennem
varmeveksleren hvis monteret. Tilslut ikke varmeveksleren
til en vandhane eller en kølevæske kilde, hvor vandtrykket
er ureguleret.
• Aldrig indføre frostvæske eller et organisk opløsningsmiddel
til enhver del af instrumentet. Organiske opløsningsmidler
vil forårsage uoprettelige skader på enheden!
• Betjen ikke med buffer temperaturer over de maksimale
angivne tekniske specifikationer. Overophedning vil forårsage uoprettelige skader på enheden!
Fan Sensor Kit:
• Advarsel! Fordi dette instrument kan udvikle tilstrækkelig
spænding og strøm til frembringelse af en dødelig chok, skal
der udvises forsigtighed ved driften.
• Dette instrument er designet i overensstemmelse med
EN61010-1: 2001 elektrisk sikkerhed standard. I kke desto
mindre bør det kun bruges af korrekt uddannet operatører.
Læs hele denne manual inden du bruger instrumentet, og
brug kun i henhold til instruktioner ne.
• Instrumentet skal altid bruges sammen med jordledningen
af netledningen korrekt jordet til jorden ved stikkontakten.
• Brug kun ubeskadigede elektriske ledninger og udstyr
specifikt for de spændinger, du vil bruge. Alt udstyr, der
tilsluttes høj spænding bør være i overensstemmelse med
EN61010-1: 2001.
• Hold instrumentet så tør og ren som muligt. Tør regelmæssigt med en blød, fugtig k lud. Lad instrumentet tørre helt
inden brug.
• Brug ikke instrumentet i ekstrem luftfugtighed (over
80%). Undgå kondens ved at lade enheden ækvilibrere til
stuetemperatur, når du tager instrumentet fra et koldere til
et varmere miljø.
• At tillade tilstrækkelig køling, sørge for, at ventilationsåbningerne i instrumentet ikke er dækket.
Belangrijke informatie – Dutch
SQ33:
• Als deze apparatuur wordt gebruikt op een manier niet
bepaald door Hoefer, Inc de bescherming die door de apparatuur worden aangetast.
• Dit instrument is ontworpen voor gebruik binnenshuis laboratorium gebruik.
• Alleen accessoires en onderdelen zijn goedgekeurd of
geleverd door Hoefer, Inc kan worden gebruikt voor
exploitatie, het onderhoud en reparatie van dit produc t.
• Gebruik alleen een voeding die CE-markering of de
veiligheid gecertificeerd is door een nationaal erkende
testlaboratorium.
• De veiligheid deksel moet in plaats voordat u de voeding
leidt tot een voeding.
• Schakel alle stroomtoevoer regelt af en haal de stekker leidt
voordat u de veiligheid deksel.
• Alleen circuleren water of 50/50 water/ethyleenglycol door
de warmtewisselaar indien aanwezig. Maak de warmtewisselaar niet aan op een waterk raan of een koelvloeistof bron
waar het water zwak is niet gereguleerd.
• Steek nooit antivries of organische oplosmiddelen op enig
deel van het instrument. Organische oplosmiddelen veroorzaakt onherstelbare schade aan het apparaat!
• Werk niet met buffer temperaturen boven de maximale
opgegeven technische specificaties. Oververhitting veroorzaakt onherstelbare schade aan het apparaat!
Fan Sensor Kit:
• Waarschuwing! Omdat dit instrument kan voldoende spanning
ontwikkelen en stroom naar een dodelijke schok produceren,
moet voorzichtigheid worden betracht bij de werking ervan.
• Dit instrument is ontworpen in overeenstemming met de
EN61010-1: 2001 elektrische veiligheidsnorm. Toch moet
alleen worden gebruikt door goed opgeleid exploitanten.
Lees de volledige handleiding voordat u het instrument en
gebruik alleen volgens de instructies.
• Het instrument moet altijd gebruikt worden met de aarddraad van het netsnoer goed geaard naar de aarde op het
stopcontact.
• Gebruik alleen onbeschadigde elektrische draad en apparatuur die specifiek zijn voor de spanningen die u wilt
gebruiken. Alle apparatuur die op hoge spanning moet in
overeenstemming zijn met de EN61010-1: 2001.
• Houd het instrument zo droog en schoon mogelijk. Veeg
regelmatig met een zachte, vochtige doek. Laat het instrument volledig drogen voor gebruik.
• Gebruik het instrument niet in extreem hoge
luchtvochtigheid (boven 80%). Condensatie voorkomen
door te laten de eenheid evenwicht tot omgevingstemperatuur bij het nemen van het instrument van een kouder naar
een warmere omgeving.
• Om voldoende koeling mogelijk te maken, ervoor te
zorgen dat de ventilatieopeningen van het instrument niet
wordengedekt.
piii
•
Tärkeitä tietoja – Finnish
SQ33:
• Jos laitetta käytetään tavalla ei määritelty Hoefer, Inc.
antama suojaus saattaa heiketä.
• Tämä väline on suunniteltu sisätiloissa laboratorion käyttöön.
• Vain lisätarvikkeet ja osat hyväksytty tai toimittamia Hoefer,
Inc. voi käyttää käytöstä, ylläpidosta ja huoltoa tuote.
• Käytä vain virtalähdettä, joka on CE-merkitty ja turvallisuutta
sertifioitu kansallisesti tunnustettu testauslaboratorio.
• Turvallisuus Kannen on oltava paikoillaan ennen kuin kytket
virtalähteen johtaa virtalähteeseen.
• Käännä kaikki virtalähteen ohjaa virta ja irrota virtajohdot
ennen kuin irrotat turvallisuutta k ansi.
• Kierrätä vain vettä tai 50/50 vesi/etyleeniglykoli lämmönvaihtimen läpi, jos varusteena. Älä kytke lämmönvaihtimen
vesihanaan tai jäähdytysnestettä lähde, josta vesi paine on
sääntelemätöntä.
• Koskaan käyttöön pakkasnestettä tai mik ä tahansa orgaaninen liuotin mihin tahansa osaan väline. Orgaaniset liuottimet aiheuttaa korjaamatonta vahinkoa laitetta!
• Älä käytä puskuria lämpötiloissa enimmäiskokoon tekniset
tiedot. Ylikuumeneminen korjaamatonta vahinkoa laitetta!
Fan Sensor Kit:
• Varoitus! Koska tämä väline voi kehittyä riittävän jännitteen
ja virran tuottamiseen tappavan iskun, on oltava varovaisia
sen toiminnassa.
• Tämä väline on suunniteltu mukaisesti EN61010-1: 2001
sähköturvallisuus standardia. On kuitenkin sy ytä käyttää
vain asianmukaisesti koulutetut operaattorit. Lue tämä
käyttöohje ennen kuin käytät laitetta ja käytä vain ohjeiden
mukaan.
• Laite on aina käytettävä maajohtoa virtajohto kunnolla
maadoitettu Earth pistorasiaan.
• Käytä vain vahingoittumattomia sähköjohdot ja laitteet
spesifisiä jännitteiden käytät. Kaikki laitteet on kytketty suurjännite olisi noudatettava EN61010-1: 2001.
• Pidä laite kuivana ja puhtaana kuin mahdollista. Pyyhi säännöllisesti pehmeällä, kostealla liinalla. Anna laitteen k uivua
täysin ennen käyttöä.
• Älä käytä laitetta hyvin kosteissa (yli 80%). Vältä kosteuden
tiivistymistä antamalla yksikön tasaantua ympäristön
lämpötilaan, kun otetaan instrumentti kylmempi
lämpimämpään ympäristöön.
• Sallia riittävä jäähdytys, että tuuletusaukkojen välineen
eivät kuulu.
Informations importantes –
French
SQ33:
• Si cet équipement est utilisé d’une manière non spécifiée
par Hoefer, Inc la protection fournie par l’équipement peut
être altérée.
• Cet instrument est conçu pour une utilisation en laboratoire
à l’intérieur uniquement.
• Seuls les accessoires et les pièces approuvées ou fournies
par Hoefer, Inc peut être utilisé pour le fonctionnement, la
maintenance et l’entretien de cet appareil.
• Utilisez uniquement un bloc d’alimentation qui est marqué
CE ou la sécurité certifié par un laboratoire reconnu au
niveau national.
• Le couvercle de sécurité doit être en place avant de
brancher les fils d’alimentation à une alimentation électrique.
• Mettez tous alimentation contrôle hors tension et débranchez les câbles d’alimentation avant de retirer le couvercle
de sécurité.
• Faire circuler que de l’eau ou 50/50 eau/éthylène glycol dans
l’échangeur de chaleur s’il en est équipé. Ne branchez pas
l’échangeur de chaleur à un robinet d’eau ou toute autre
source de liquide de refroidissement où la pression de l’eau
n’est pas réglementé.
• Ne jamais introduire d’antigel ou tout autre solvant organique dans n’importe quelle partie de l’instrument. Solvants
organiques provoquent des dommages irréparables à
l’appareil!
• Ne faites pas fonctionner avec des températures au-dessus
des tampons maximales spécifiées spécifications techniques.
Une surchauffe peut causer des dommages irréparables à
l’appareil!
Fan Sensor Kit:
• Attention! Parce que cet instrument peut se développer de
tension et une intensité suffisantes pour produire un choc
mortel, il faut être prudent dans son fonctionnement.
• Cet instrument est conçu conformément à la norme
EN61010-1: 2001 norme de sécurité élec trique. Néanmoins,
il doit être utilisé uniquement par des opérateurs dûment
formés. Lisez ce manuel avant d’utiliser l’instrument et
d’utiliser uniquement selon les instructions.
• L’instrument doit toujours être utilisé avec le fil de terre du
cordon d’alimentation correctement mis à la terre à la prise
de courant.
• Utilisez uniquement des fils en bon état élec trique et des
équipements spécifiques pour les tensions que vous allez
utiliser. Tous les équipements connec tés à haute tension
doivent être conformes à la norme EN61010-1: 2001.
• Gardez l’appareil au sec et propre que possible. Essuyez
régulièrement avec un chiffon doux et humide. Laissez
l’instrument sécher complètement avant utilisation.
• Ne pas faire fonctionner l’instrument dans une atmosphère
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•
très humide (plus de 80%). Éviter la condensation en laissant l’unité s’équilibrer à la température ambiante lors de la
prise de l’instrument à partir d’un froid à un environnement
chaud.
• Afin de permettre un refroidissement suffisant, assurez-vous
que les orifices de l’appareil ne sont pas couverts.
Wichtige information – German
SQ33:
• Wenn dieses Gerät in einer Weise, die nicht durch Hoefer,
Inc. angegeben verwendet wird, der Schutz des Geräts
beeinträchtigt sein.
• Dieses Instrument ist für den Innen-Einsatz im Labor
konzipiert.
• Nur Zubehör und Teile zugelassen oder von Hoefer, Inc.
versorgt kann für den Betrieb verwendet werden, Pflege
und Wartung dieses Produkts.
• Verwenden Sie nur eine Stromversorgung, die
CE-Kennzeichnung oder die Sicherheit zertifizier t ist durch
ein staatlich anerkanntes Prüflabor.
• Die Sicherheit Deckel muss vorhanden sein, bevor Sie die
Stromversorgung führt zu einer Stromversorgung.
• Schalten Sie alle Stromversorgung steuert aus und ziehen
Sie den Netzstecker führt, bevor Sie den Sicherheitsdeckel.
• Zirkulieren nur Wasser oder 50/50 Wasser/Ethylenglykol
durch den Wärmetauscher falls vorhanden. Schließen Sie
den Wärmetauscher an einen Wasserhahn oder einen Kühlmittel-Quelle, wo der Wasserdruck ist ungeregelt.
• Nie einzuführen Frostschutzmittel oder keine organischen
Lösungsmittel in einen Teil des Instruments. Organische
Lösemittel verursacht irreparable Schäden am Gerät!
• Nicht mit Puffer Temperaturen oberhalb der maximal
angegebenen technischen Spezifikationen arbeiten. Überhitzung kann zu irreparablen Schäden am Gerät!
Fan Sensor Kit:
• Warnung! Da dieses Instrument genügend Spannung
entwickeln kann und Strom, um einen tödlichen Stromschlag zu erzeugen, muss darauf in seinem Betrieb ausgeübt
werden.
• 2001 elektrische Sicherheit Standard: Dieses Gerät ist in
Übereinstimmung mit der EN61010-1 ausgelegt. Dennoch
sollte sie nur von entsprechend geschultem Operatoren
verwendet werden. Lesen Sie das gesamte Handbuch, bevor
Sie das Gerät und verwenden Sie nur nach den Anweisungen.
• Das Gerät muss immer mit dem Schutzleiter des Stromkabels korrekt mit der Erde an der Steckdose geerdet werden.
• Verwenden Sie nur unbeschädigte elektrische Drähte und
spezielle Ausrüstungen für die Spannungen, die Sie verwenden. 2001: Alle Geräte, die an hohe Spannung sollte in
Übereinstimmung mit EN61010-1 sein.
• Halten Sie das Gerät trocken und sauber wie möglich.
Wischen Sie regelmäßig mit einem weichen, feuchten Tuch.
Lassen Sie das Gerät vollständig trocknen, bevor Gebrauch.
• Betreiben Sie das Instrument nicht in extreme Luftfeuchtigkeit (über 80%). Kondensation vermeiden, indem das Gerät
auf Raumtemperatur äquilibrieren, wenn man das Gerät von
einer kalten in eine wärmere Umgebung.
• Um eine ausreichende Kühlung zu ermöglichen, stellen
Sie sicher, dass die Lüftungsschlitze des Gerätes nicht
verdecktsind.
Informazioni importanti – Italian
SQ33:
• Se l’apparecchiatura viene utilizzata in un modo non specificato dal Hoefer, Inc. la protezione fornita dallo strumento
può essere compromessa.
• Questo strumento è stato progettato per l’uso in laboratorio
interno.
• Solo accessori e ricambi approvati o forniti da Hoefer, Inc.
può essere utilizzato per il funzionamento, la gestione e la
manutenzione di questo prodotto.
• Utilizzare solo un alimentatore che la marcatura CE o di
sicurezza certificato da un laboratorio di prova riconosciuto
a livello nazionale.
• Il coperchio di sicurezza deve essere in posizione prima di
collegare l’alimentazione porta ad un alimentatore.
• Accendere tutti alimentatore controlla e scollegare i cavi di
alimentazione prima di rimuovere il coperchio di sicurezza.
• Circolare solo acqua o 50/50 di acqua/glicole etilenico attraverso lo scambiatore di calore se presente. Non collegare
lo scambiatore di calore ad un rubinetto dell ’acqua o di
qualsiasi altra fonte del liquido di raffreddamento in cui la
pressione dell’acqua è regolamentata.
• Non introdurre antigelo o qualsiasi solvente organico in
qualsiasi parte dello strumento. Solventi organici può
causare danni irreparabili alla centralina!
• Non utilizzare con temperature sopra i massimi tampone
specifiche tecniche indicate. Il surriscaldamento può causare
danni irreparabili alla centralina!
Fan Sensor Kit:
• Attenzione! Dal momento che questo strumento può sviluppare una tensione e corrente sufficiente a produrre uno
shock, la cura deve essere esercitata nel suo funzionamento.
• Questo strumento è stato progettato secondo la EN61010-1:
2001 standard di sicurezza elettrica. Tuttavia, deve essere
utilizzato solo da parte di personale addestrato. Leggere il
presente manuale prima di utilizzare lo strumento e utilizzare solo secondo le istruzioni.
• Lo strumento deve essere sempre utilizzato con il cavo di
terra del cavo di alimentazione di messa a terra a terra alla
presa di corrente.
• Utilizzare solo cavo elettrico danneggiato e attrezzature
specifiche per le tensioni si intende utilizzare. Tutte le apparecchiature collegate ad alta tensione deve essere in accordo
con EN61010-1: 2001.
pv
•
• Tenere lo strumento più asciutto e pulito possibile. Pulire
regolarmente con un panno morbido e umido. Lasciare asciugare completamente lo strumento prima dell’uso.
• Non utilizzare lo strumento in alta umidità (superiore
all’80%). Evitare la formazione di condensa lasciando che
l’unità raggiunga la temperatura ambiente quando prende
lo strumento da un freddo a un ambiente più caldo.
• Per consentire un sufficiente raffreddamento, assicurarsi che
le prese d’aria dello strumento non sono coperti.
Viktig informasjon – Norwegian
SQ33:
• Hvis dette utstyret brukes på en måte som ikke er spesifisert
av Hoefer, Inc. beskyttelsen av utstyret kan bli svek ket.
• Dette instrumentet er beregnet for innendørs laboratorium
bruk.
• Bare tilbehør og deler som er godkjent eller levert av Hoefer,
Inc. kan brukes til drif t, vedlikehold og vedlikehold av dette
produktet.
• Bruk kun en strømforsyning som er CE-merket eller sikkerhet
sertifisert av et nasjonalt anerkjent testlaboratorium.
• Sikkerheten lokk må være på plass før du kobler til strømforsyningen fører til en strømforsyning.
• Slå all strømtilførsel styrer av og koble strømledningene før
du tar sikkerheten lokket.
• Sirkuler bare vann eller 50/50 vann/etylenglykol gjennom
varmeveksleren hvis slik utstyrt. Ikke koble varmeveksler
til en vannkran eller kjølevæske k ilde hvor vanntrykket er
uregulert.
• Aldri introdusere frostvæske eller ethvert organisk oppløsningsmiddel inn i noen del av instrumentet. Organiske
løsemidler vil føre til uopprettelig skade på enheten!
• Opererer ikke med buffer temperaturer over den maksimale
angitte tekniske spesifikasjoner. Overoppheting vil forårsake
uopprettelig skade på enheten!
Fan Sensor Kit:
• Advarsel! Fordi dette instrumentet kan utvikle tilstrekkelig
spenning og strøm for å produsere en dødelig støt, må det
utvises forsiktighet i sin drift.
• Dette instrumentet er utformet i samsvar med EN61010-1:
2001 elsikkerhet standard. Likevel bør det bare brukes av
riktig opplæring operatører. Les hele denne håndboken før
du bruker instrumentet og bruk kun i henhold til instruksjonene.
• Instrumentet må alltid brukes sammen med jordledningen
av strømledningen riktig jordet til jorden på stikkontakten.
• Bruk bare uskadde elektrisk ledning og utstyr bestemt for
spenninger du vil bruke. Alt utstyr som er koblet til høy
spenning bør være i samsvar med EN61010-1: 2001.
• Hold instrumentet så tørt og rent som mulig. Tørk regelmessig med en myk, fuktig k lut. La instrumentet tørke helt før
bruk.
• Ikke bruk instrumentet i ekstrem luftfuktighet (over 80%).
Unngå kondens ved å la enheten tilpasse seg til omgivelsestemperaturen når du tar instrumentet fra en k aldere til
varmere omgivelser.
• Å tillate tilstrekkelig kjøling, må du sørge for at ventilasjonsåpningene på instrumentet ikke er dekket.
Wazne informacje – Polish
SQ33:
• Jeśli urządzenie jest używane w sposób, któr y nie został
określony Hoefer, Inc ochrona zapewniana przez urządzenie
może być zaburzona.
• Instrument ten jest przeznaczony do użytku w pomieszczeniach laboratoryjnych tylko.
• Tylko części i akcesoria zatwierdzone lub dostarczone przez
Hoefer, Inc mogą być używane do obsługi, konser wacji i
serwisowania tego produktu.
• Należy używać wyłącznie zasilacza, który jest oznaczony
znakiem CE lub bezpieczeństwa cer tyfikowane przez
uznanym w kraju laboratorium.
• Pokrywa bezpieczeństwa musi być na miejscu przed
podłączeniem zasilania prowadzi do zasilania.
• Włącz wszystkie Zasilacz kontroluje i odłączyć pr zewody
zasilania przed zdjęciem pokry wy bezpieczeństwa.
• Krążyć wodą lub 50/50 glikolu etylenowego/wody przez
wymiennik ciepła, jeśli tak wyposażone. N ie należy
podłączać do wymiennika ciepła do zaworu wody lub
każdego źródła chłodziwa, gdzie ciśnienie wody jest nieuregulowany.
• Nigdy nie wprowadzać zamarzaniu ani organicznych
rozpuszczalników w jakiejkolwiek części urządzenia.
Rozpuszczalniki organiczne, spowoduje nieodwracalne
uszkodzenie urządzenia!
• Nie działają w temperaturach powyżej maksimum buforowych określonych specyfikacjach technicznych. Przegrzanie
może spowodować nieodwracalne uszkodzenie urządzenia!
Fan Sensor Kit:
• Ostrzeżenie! Ponieważ instrument ten może rozwinąć
wystarczające napięcie i prąd do wytworzenia śmiertelną
prądem, należy zachować ostrożność w jej działania.
• Instrument ten został zaprojektowany zgodnie z EN61010-1:
2001 elektryczne standard bezpieczeństwa. Należy jednak
być używane tylko przez odpowiednio przeszkolonych
operatorów. Przeczytaj całą instrukcję przed użyciem instrumentu i używać tylko zgodnie z instrukcją.
• Urządzenie musi być zawsze używane z uziemiający przewodu zasilającego prawidłowo uziemione w gniazdku
sieciowym.
• Używaj tylko nieuszkodzony elektrycznych przewodów i
sprzętem dla napięć będzie uży wać. Wszystkie urządzenia
podłączone do wysokiego napięcia powinny być zgodne z
EN61010-1: 2001.
pvi
•
• Zachowaj instrument, suche i czyste jak to możliwe.
Przecierać regularnie miękką, wilgotną szmatką. Niech
instrumentem wyschnąć przed użyciem.
• Nie używaj instrumentu w dużej wilgotności (powyżej 80%).
Uniknąć kondensacji, pozwalając urządzenie wyrównania
temperatury otoczenia podczas wykonywania instrumentu z
chłodniejszego do cieplejszego.
• Aby umożliwić odpowiednie chłodzenie, upewnij się, że
otwory w instrumencie nie są objęte.
Informação importante –
Portuguese
SQ33:
• Se este equipamento for utilizado de uma forma não especificada pelo Hoefer, Inc., a proteção fornecida pelo equipamento poderá ser prejudicada.
• Este instrumento foi projetado para uso em laboratório no
interior.
• Somente acessórios e peças aprovadas ou fornecidos por
Hoefer, Inc. podem ser utilizados para a operação, manutenção e atendimento a este produto.
• Usar apenas uma fonte de alimentação que a marcação CE
ou de segurança certificado por um laboratório reconhecido
nacionalmente.
• A tampa de segurança devem estar no local antes de ligar o
fornecimento de energia leva a uma fonte de alimentação.
• Vire toda a oferta de energia controla e desconecte os cabos
de alimentação antes de remover a tampa de segurança.
• Circulam apenas água ou 50/50 de água/etileno glicol
através do permutador de calor, se assim equipado. Não
ligue o trocador de calor para uma torneira de água ou
qualquer outra fonte de refrigerante, onde a pressão da
água é regulamentada.
• Nunca introduzir anticongelante ou qualquer solvente
orgânico em qualquer parte do instrumento. Solventes
orgânicos podem provocar danos irreparáveis para a
unidade!
• Não opere com temperaturas acima de buffer o máximo
especificado especificações técnicas. Superaquecimento irá
causar danos irreparáveis a unidade!
Kit Sensor fã:
• Atenção! Porque este instrumento pode desenvolver tensão
e corrente suficiente para produzir um choque letal, o
cuidado deve ser exercido em sua operação.
• Este instrumento foi projetado de acordo com a EN61010-1:
2001 padrão de segurança elétrica. No entanto, ele deve ser
usado apenas por operadores devidamente treinados. Leia
todo o manual antes de usar o instrumento e usar apenas de
acordo com as instruções.
• O instrumento deve ser sempre usado com o fio terra do
cabo de alimentação corretamente aterrado na tomada.
• Use apenas fios danificados elétrica e equipamentos espe -
cíficos para as tensões que você irá usar. Todos os equipamentos conectados à alta tensão deve estar de acordo com
EN61010-1: 2001.
• Mantenha o instrumento o mais seco e limpo possível.
Limpe com um pano macio e úmido. Deixe o instrumento
secar completamente antes de usar.
• Não utilizar o aparelho de extrema umidade (acima de
80%). Evitar a condensação, deixando a unidade de atingir a
temperatura ambiente ao tirar o instrumento a par tir de um
mais frio para um ambiente mais quente.
• Para permitir uma refrigeração suficiente, garantir que as
aberturas do instrumento não são cobertos.
Información importante – Spanish
SQ33:
• Si este equipo se utiliza de una manera no especificada
por Hoefer, Inc., la protección proporcionada por el equipo
puede verse afectada.
• Este instrumento está diseñado para uso en laboratorio en
interiores.
• Sólo los accesorios y partes homologado o suministrado
por Hoefer, Inc. puede ser utilizado para el funcionamiento,
mantenimiento y reparación de este producto.
• Utilice solamente una fuente de alimentación que lleva la
marca CE o certificado de seguridad por un laboratorio de
pruebas reconocido nacionalmente.
• La tapa de seguridad deben estar en su lugar antes de
conectar la fuente de alimentación conduce a una fuente de
alimentación.
• Gire todas las fuentes de energía controla y desconecte los
cables de alimentación antes de retirar la tapa de seguridad.
• Circule sólo agua o 50/50 de agua/glicol de etileno a través
del intercambiador de calor si lo tiene. No conectar el intercambiador de calor a un grifo de agua o de cualquier fuente
de refrigerante donde la presión del agua es regulada.
• Nunca introducir anticongelante o cualquier disolvente
orgánico en ninguna parte del instrumento. Los solventes
orgánicos puede causar daños irreparables en el aparato!
• No trabajar con temperaturas por encima de los máximos de
amortiguamiento especificados especificaciones técnicas.
El recalentamiento puede causar daños irreparables en el
aparato!
Fan Kit Sensor:
• ¡Advertencia! Debido a que este instrumento puede
desarrollar suficiente voltaje y corriente para producir una
descarga eléctrica mortal, se debe tener cuidado en su
funcionamiento.
• Este instrumento está diseñado de acuerdo con la norma
EN61010-1: 2001 norma de seguridad eléctrica. No obstante,
debe ser utilizado sólo por operadores debidamente
capacitados. Lea este manual en su totalidad antes de usar
el instrumento y utilizar solamente de acuerdo con las
instrucciones.
pvii
•
• El instrumento se debe utilizar siempre con la toma de tierra
del cable de alimentación correctamente conectado a tierra
en la caja de enchufe.
• Use sólo cables eléctricos intactos y equipo específico para
las tensiones que va a utilizar. Todos los equipos conectados
a alta tensión debe ser de acuerdo con la norma EN61010-1:
2001.
• Mantenga el instrumento lo más seca y limpia posible.
Limpie periódicamente con un paño suave y húmedo. Deje
que el aparato se seque completamente antes de su uso.
• No utilice el instrumento en condiciones de extrema
humedad (superior al 80%). Evite la condensación permitiendo que la unidad alcance la temperatura ambiente en
el momento de tomar el instrumento a una más fría que la
temperatura normal.
• Para permitir la refrigeración adecuada, asegúrese de que
los orificios de ventilación del equipo no están cubiertos.
Viktig information – Swedish
SQ33:
• Om denna utrustning används på ett sätt som inte specificeras av Hoefer, Inc. skydd som utrustningen kan försämras.
• Detta instrument är avsett för inomhusbruk laboratorium
bruk.
• Endast tillbehör och reservdelar godkända eller levereras av
Hoefer, Inc. kan användas för drift, underhåll och ser vice av
denna produkt.
• Använd endast en strömkälla som är CE-märkt eller säkerhet
certifierad av ett nationellt erkänt testlaboratorium.
• Säkerheten Locket måste sitta på plats innan du ansluter
strömförsörjningen leder till en strömkälla.
• Slå alla strömförsörjningen kontrollerar av och kopplar leder
innan du tar bort säker heten locket.
• Cirkulera endast vatten eller 50/50 vatten/etylenglykol
genom värmeväxlaren, om så utrustad. Anslut inte
värmeväxlaren till en vattenkran eller någon k ylvätska källa
där vattnet trycket är oreglerad.
• Aldrig införa frostskyddsmedel eller organiskt lösningsmedel
i någon del av instrumentet. Organiska lösningsmedel kan
orsaka irreparabla skador på enheten!
• Fungerar inte med buffert temperaturer över de specificerade maximala tekniska specifikationer. Överhettning kan
orsaka irreparabla skador på enheten!
Fan Sensor Kit:
• Varning! Eftersom detta instrument kan utveckla tillräcklig
spänning och ström för att producera en dödlig chock,
måste man vara försiktig i sin verksamhet.
• Detta instrument är utformat i enlighet med EN61010-1:
2001 elsäkerhet standard. Det bör dock endast användas av
utbildad operatörer. Läs hela den här handboken innan du
använder instrumentet och endast använda enligt instruktionerna.
• Instrumentet skall alltid användas med jordkabel nätsladden
korrekt jordad till jorden vid vägguttaget.
• Använd endast oskadade elektrisk tråd och utrustning som
är specifik för de spänningar du kommer att använda. All
utrustning är ansluten till högspänning sk all ske i enlighet
med EN61010-1: 2001.
• Håll instrumentet så torr och ren som möjligt. Torka regelbundet med en mjuk, fuktig trasa. Låt instrumentet torka
helt före användning.
• Använd inte instrumentet i hög luftfuktighet (över 80%).
Undvik kondens genom att låta enheten jämvikt till rumstemperatur när du tar instrumentet från en kallare till en
varmare omgivning.
• För att möjliggöra tillräcklig kylning, se till att ventilerna i
instrumentet inte omfattas.
•
pviii
Waste Electrical and Electronic Equipment (WEEE)
English
French
German
Italian
Spanish
This symbol indicates that the waste of electrical and
electronic equipment must not be disposed as unsorted
municipal waste and must be collected separately. Please
contact an authorized representative of the manufacturer
for information concerning the decommissioning of
your equipment.
Ce symbole indique que les déchets relatifs à l’équipement
électrique et électronique ne doivent pas être jetés comme
les ordures ménagères non-triées et doivent être collectés
séparément. Contactez un représentant agréé du fabricant
pour obtenir des informations sur la mise au rebut de
votre équipement.
Dieses Symbol kennzeichnet elektrische und elektronische
Geräte, die nicht mit dem gewöhnlichen, unsortierten
Hausmüll entsorgt werden dürfen, sondern separat
behandelt werden müssen. Bitte nehmen Sie Kontakt mit
einem autorisierten Beauftragten des Herstellers auf, um
Informationen hinsichtlich der Entsorgung Ihres Gerätes
zu erhalten.
Questo simbolo indica che i rifiuti derivanti da
apparecchiature elettriche ed elettroniche non devono essere
smaltiti come rifiuti municipali indifferenziati e devono invece
essere raccolti separatamente. Per informazioni relative alle
modalità di smantellamento delle apparecchiature fuori uso,
contattare un rappresentante autorizzato del fabbricante.
Este símbolo indica que el equipo eléctrico y electrónico no
debe tirarse con los desechos domésticos y debe tratarse por
separado. Contacte con el representante local del fabricante
para obtener más información sobre la forma de desechar
el equipo.
Swedish
Denna symbol anger att elektriska och elektroniska
utrustningar inte får avyttras som osorterat hushållsavfall och
måste samlas in separat. Var god kontakta en auktoriserad
tillverkarrepresentant för information angående avyttring
av utrustningen.
pix
•
Warning
These units are capable of delivering potentially
lethal voltage when connected to a power supply
and are to be operated only by qualified technically trained personnel.
Please read the entire operator’s manual thoroughly before operating this unit.
These units comply with the statutory EC safety
requirements:
• 98/79/EC In Vitro Diagnostic Directive of
October 27, 1998
• 73/23/EEC: LOW VOLTAGE DIRECTIVE:
IEC 1010-1:1990 plus AMENDMENT 1:1992
• EN 61010-1:1993/BS EN 61010-1:1993
89/336/EC: THE EMC DIRECTIVE FOR
ELECTROMAGNETIC COMPATIBILITY
Hoefer single-plate large format gel electrophoresis units are designed to give long service and
reproducible results in your laboratory. A few
moments spent reading these instructions will
ensure that your expectations are reflected in the
successful use of the apparatus.
First check that the apparatus has been received
complete and undamaged following shipment.
Any faults or losses must be reported to Hoefer
immediately. Hoefer cannot accept responsibility
for goods returned without prior notification.
Refer to the packing list and check that all
components and accessories are present.
Please retain all packaging and materials until
the warranty period has expired.
px
•
1. SQ33 Function and
Description
The Hoefer® SQ33 Sequencer offers a wide gel
format for increased sample capacity and Shark’s
Tooth combs to ensure optimal band resolution. The SQ33 Sequencer is perfect for DNA
sequencing, DNAse and RNase footprinting, and
heteroduplex or oligonucleotide analysis. The
removable lower buffer chamber combined with
the upper buffer chamber drainage tap make
buffer disposal easy and safe.
1.1 Unpacking
Unwrap all packages carefully and compare
contents with the packing list, making sure all
items arrived. If any part is missing, contact
your local sales office. Inspect all components
for damage that may have occurred while the
unit was in transit. If any part appears damaged,
contact the carrier immediately. Be sure to keep
all packing material for damage claims or to use
should it become necessary to return the unit.
1.2 SQ33 Sequencer Components
Description Quantity
Running Tank + Lid 1
Plain Glass Plates 2
Notched Glass Plates 2
0.35 mm Melinex Spacers 2
0.35 mm Shark’s Tooth Comb, 48 well 1
p1
•
2. SQ33 Specifications
Unit Dimensions (W × D × H) 63 × 21.5 × 50 cm
Plate Dimensions (W × H × D) 33 × 41 × 0.4 cm
Standard Spacer Dimensions (W × H × D) 10 × 410 × 0.35 mm
Active Gel Dimensions (W × H) 29 × 38.5 cm
Maximum Sample Capacity 1 × 96
Recommended Buffer Volume Upper Buffer Chamber: 1200 ml
Lower Buffer Chamber: 1200 ml
Recommended running conditions for 6% Power: 45 – 55 W
denaturing PAGE gel using passive ventilation Gel Temperature: 50 – 60 °C
at 20 °C ambient temperature: Time: 2.5 – 3 h
Recommended running conditions for 6% Power: 75 W
denaturing PAGE gel using FHS-KIT at 20 °C Gel Temperature: 55 °C
ambient temperature: Time: 1.75 h
2.1 Fan Sensor Kit Specifcations
Temperature Control Unit:
Dimensions (H × W × D) 80 × 96 × 140 mm
Temperature Control Range 0 – 100 °C
Controller Accuracy +/– 2%
Weight 1.5 kg
Fan:
Dimensions (H × W × D) 38 × 120 × 120 mm
Operating Speed 2,700 rpm
Flow Rate > 84 CFM
Noise Rating 36 dBA
p2
•
2.2 Environmental Conditions
• This apparatus is intended for indoor use only.
• This apparatus can be operated safely up to an altitude of 2,000 m.
• The normal operating temperature range is between
4 °C and 65 °C.
• Maximum relative humidity 80% for temperatures
up to 31 °C decreasing linearly to 50% relative
humidity at 40 °C.
• The apparatus is rated POLLUTION DEGREE 2 in
accordance with IEC 664. POLLUTION DEGREE 2,
states that: “Normally only non-conductive pollution
occurs. Occasionally, however, a temporary conductivity caused by condensation must be expected”.
p3
•
3. SQ33 Operating
Instructions
3.1 Regent Preparation and Gel Volumes
For reproducibility and uniform polyacrylamide
cross-linking we recommend deionizing, degassing and filtration of acrylamide gel solutions
prior to use. Acrylamide solutions should be
stored in a cool, dark environment such as a
refrigerator and allowed to reach room temperature prior to pouring. Avoid exposure to heat
and sunlight.
Polymerization conditions should be adjusted
to effect polymerization within about 5 – 10
minutes. Test a small volume in a vial prior to
pouring the gel. As a rough guide 100 ml of
degassed 6% acrylamide gel will set in about 5
minutes at room temperature when gently mixed
with 450 μl of freshly prepared 10% (w/v)
Ammonium persulfate plus 200 μl TEMED.
The setting time increases to about 10 minutes
if the TEMED volume is reduced to 100 μl and
to approximately 15 minutes with 75 μl. The
amount of catalysts may need to be reduced
under warm conditions. Do not pour under
direct sunlight.
The SQ33 Sequencer, using 0.35 mm thick
spacers, requires approximately 60 mls of
acrylamide solution.
p4
•
3.2 Gel Pouring
1
Remove any greasy fingerprints or water on the edges
of the glass plates by wiping with acetone. Lay the
plain glass plate on a flat surface and arrange the
spacers flush with the edges of the plate. Carefully lay
the notched glass plate on top and clamp the plates
together. The gel sandwich is now ready for pouring.
Refer to the illustration in Figure 1, page 6.
2
Fill a 60 ml syringe with gel mix being careful not to
aerate the solution.
3
Hold the syringe as vertical as possible and using
a steady continuous motion expel the gel mix along
the notched area moving the syringe nozzle repeatedly from one side of the notch to the other. The gel
mix should form a continuous pool along the top of
the gel space and by capillary action it will move
down between the plates. Do not overfill the notched
area — the pool should be approximately half the
height of the glass ears. Alternatively do not let the
pool run dry or air bubbles will be introduced between
the glass plates. The boundary of the gel should
migrate as a straight line. The gel plates can be
tapped slightly behind the moving gel boundary, using
your fingers, to prevent any bubble formation. Do not
use any hard or metal objects.
4
When the gel boundary reaches the bottom of the
glass plates, remove the surplus gel from the notched
area. Failure to do so will result in the gel mix dripping from the bottom end. Insert the required comb.
5
If you are using a comb with square wells insert the
teeth without trapping bubbles. If you are using a
sharks tooth comb insert the flat face of the comb at
a slight angle to prevent bubbles from trapped. Add a
few drops of gel mix if necessary. Carefully straighten
the comb so that it is parallel to the top of the gel
plate and reaches 3 – 5 mm below the notched area.
p5
•
Figure 1. Pouring gels horizontally.
Tap firmly with fingers over
areas such as this
• Hold syringe vertically.
• Move across notched area to maintain a
pool of gel mix.
• Do not overfill.
• Do not attempt to force gel mix down
between plates with the syringe jet.
• When gel boundary has reached the end
remove surplus gel mix from notched
area. Leave just a small amount to
ensure bubbles don’t get trapped under
the comb.
• The comb area may be clamped with
bulldog clips.
• Do not let the clips protrude beyond the
line of the comb otherwise get thickness variation and hence lane distortion
may occur.
• When gel has set carefully remove comb
and insert it teeth first (being careful not
to damage points) so it just contacts the
gel surface.
• The gel is then ready to be attached to
the main unit and loaded with samples.
Notched
Area
3 – 5 mm
p6
•
6
The gel polymerization should occur within 10
minutes but allow the gel to polymerize further for
at least 90 minutes. If it is to polymerize overnight,
enclose wet tissues under a cling film seal to prevent
the ends of the gel from drying out.
If you are using a square toothed comb do not remove
it until it is submerged below the running buffer.
However, if you use a Shark’s tooth comb it should be
removed before the addition of buffer. Insert it teeth
first into the gel slot so that the teeth just come into
contact with the gel and then add the running buffer.
3.3 Unit Assembly
1
Place the lower buffer chamber into the main unit.
Push it back until it stops. This ensures that the
electrode separation and hence field strength are
consistent from one run to another.
2
Remove any clamps and the bottom tape, if used.
3
Clamp the gel plate sandwich into position on the
apparatus. Do not over tighten the gel plate clamping
screws as this will lead to the glass plates breaking and also making the insertion and removal of
combs difficult.
4
The gel plate assembly will rest against the aluminum
back plate. This has been set during manufacture to be
parallel to the main frame. Do not tighten or adjust the
aluminum plate securing screws. The aluminum plate
is cushion mounted to provide good contact with the
glass plate.
5
Make sure that the upper buffer tank drainage tube
is closed.
6
Attach the electrical connectors to the buffer chambers.
p7
•
3.4 Run Conditions and Buffer Volumes
1
Into the upper buffer chamber pour 800 ml
(approximately).
IMPORTANT! Do not fill over
the maximum fill lines.
2
Into the bottom buffer chamber pour 800 ml
(approximately).
3
Ensure that there is no buffer leakage.
4
If desired pre-run the gel until the glass plates are
warm. Use the settings described below.
5
Prior to loading your samples flush out the wells with
running buffer to clear them of urea.
6
Heat the sequencing samples at 95 °C for 3 minutes,
place on ice and centrifuge 12,000 × g for 3 minutes.
Return to ice.
7
Load 1– 5 μl of sample using a suitable loading tip. If
possible avoid taking the sample from the bottom of
the tube — particulate materials may cause streaking
or smearing. Try to minimize sample dispersion, load
the sample directly onto the bottom of the well and
keep it as a thin layer.
8
Replace the safety lid ensuring it is positioned fully
down over the electrical connectors.
9
Connect and run the gel at the desired power setting.
The leads and electrical connectors are tested to
1,500 Volts and users are advised not to exceed
this voltage.
p8
•
Typically 45 – 55 Watts constant power will give good
results with passive ventilation. If the fan kit is used
the power can be increased up to 75 Watts. Ensure
that with the buffer system employed the voltage does
not exceed the maximum recommended voltage. The
resistance of the gel changes throughout electrophoresis when run at constant power so the starting and
finishing voltages will be different.
!
In general the gel temperature should reach
50 – 60 °C. At temperatures above this range there is
the risk of generating smeared bands or damaging the
gel plates.
3.5 Ending the Run
1
Isolate the power supply before removing the connectors.
2
Remove the safety lid by gripping the edges and pushing down with your thumbs on the pegs located on the
top of the unit.
3
Disconnect the leads to the upper and lower
buffer chambers.
IMPORTANT! Make sure
that the bottom tank does
not overflow.
4
Open the upper buffer tank drainage tube. The buffer
will then flow into the bottom buffer tank.
5
Remove the lower buffer chamber and carefully
discard the buffer to waste.
6
Dismantle the gel and separate the plates with a
strong, thin, broad blade.
p9
•
7
Note: Do not pry the glass plates apart at the ears.
Concentrated leverage in any one area may damage
the plates.
8
After removing the gel, clean the plates thoroughly
in neutral detergent and rinse with distilled water. A
sheet of clean foam rubber placed in the bottom of
the sink serves as a useful support and minimizes the
risk of plate damage.
9
Rinse buffer chambers in deionized water.
Never use organic solvents.
Dry the electrical connectors thoroughly with a tissue
before storing the unit.
•
p10
4. Installation and Operation of the Fan Sensor Kit
4.1 Installation
The kit consists of:
• 1 × 12 volt fan
(+4 securing screws)
• 1 × Self adhesive
temperature sensor
• 4 × Adhesive cable ties
• 1 × Temperature control unit
1
To fit the fan, simply unscrew and retain the four
screws securing the black ventilation cover on the
safety lid. Align the fan and note that the fan draws
air up through the sequencing unit. An arrow on the
fan indicates the direction of the air flow. Secure
the fan into position using the long securing screws
provided. Replace the black ventilation cover on top
of the fan so that the fan blades are not exposed.
2
Set up the polymerized sequencing gel in the unit.
Using the adhesive strip, attach the flat face of the
heat sensor to the center of the facing glass plate. It
is advisable to clean the area with a tissue soaked in
acetone prior to attachment. Do not allow acetone to
come into contact with the plastic components.
3
Route the heat sensor cable up and over the back
corner of the gel unit, securing the cable so that it
will not drag on the sensor. The fan controller is now
ready for use.
•
p11
4.2 Using the Fan Controller
1
Attach the fan connector to the back of the temperature control box.
2
Attach the heat sensor cable into the remaining
socket on the rear of the temperature control box.
3
Select the temperature you require to run the gel at,
noting that while the control unit is factory calibrated
there will be a temperature gradient across both the
glass and aluminum plate. Therefore, it is necessary
to set a temperature on the dial approximately 10 °C
lower than required at the gel plate, e.g. if you require
to run the gel at 55 °C set the dial to approximately
45 °C. Attach the mains lead to the control unit and
turn on the power. The light on the control panel indicates when the contacts are made for the fan operation. When the light is on the fan will be off. When
the fan cuts in the light will go out.
4
The fan will automatically switch on/off to maintain
the gel temperature at +/– 4 °C of the set temperature.
After monitoring the gel temperature increase the dial
setting as necessary. A temperature indicator strip on
the center of your glass plate will allow you to confirm
that your dial setting is correct.
•
p12
IMPORTANT! Acrylic plastic
is not resistant to aromatic
or halogenated hydrocarbons,
ketones, esters, alcohol’s (over
25%) and acids (over 25%),
they will cause “crazing”
especially of the UV transparent
plastic and should not be
used for cleaning. Do not use
abrasive creams or scourers. Dry
components with clean tissues
prior to use.
5. SQ33 Care and Maintenance
• To remove the safety lid, push thumbs down on
the plastic lugs and lift the lid vertically with
your fingers.
• Before use clean and dry the apparatus with
distilled water only.
• Before use, and then on a monthly basis, check
the unit for any leaks at the bonded joints. Place
the unit on a sheet of dry tissue and then fill with
distilled water only to the maximum fill line. Any
leakage will be seen on the tissue paper. If any
leakage is seen, do not attempt to repair or use
the apparatus, but notify Hoefer technical support
immediately.
• After each use, wash all components with a suitable water-soluble disinfectant, to reduce the risk
of infection.
• Ensure that the connectors are clean and dry before
usage or storage.
• The cooling plate upper surface cannot be cleaned
with acetone. Methanol and ethanol can be used.
5.1 Cleaning and Storage
• After use, thoroughly rinse all components in
deionized water and gently dry the gold electrode
connectors with a soft tissue.
• Ensure that the connectors are clean and dry before
usage or storage.
• Never use organic solvents.
5.2 Fan Sensor Kit Care and Maintenance
• The unit can be cleaned as required when the mains
supply is isolated.
• Cleaning should be carried out with a cloth
moistened with water or tissues impregnated with
70% iso-propyl alcohol. No other cleaning solutions
should be used.
• There are no internal operator serviceable parts in
this unit. If the power should fail, the unit must be
returned to the authorized service center.
p13
•
IMPORTANT! Request a copy of
the Hoefer, Inc. “Health and
Safety Declaration” form before
returning the item. No items
can be accepted for servicing
or return unless this form is
properly completed.
Note: A Return Materials
Authorization (RMA) number
must be obtained from Hoefer,
Inc, before returning any item to
Hoefer, Inc.
6. Customer Service
Information
6.1 Technical Service and Repair
Hoefer, Inc. offers complete technical support
for all of our products. If you have any questions about how to use this product, or would
like to arrange to repair it, please call or fax
your local Hoefer, Inc. representative.
Check the Hoefer, Inc. website at
www.hoeferinc.com for the distributor in your
area. Or contact us directly at:
Hoefer, Inc.
84 October Hill Road
Holliston, MA 01746
Toll Free: 1-800-227-4750
Phone: 1-508-893-8999
Fax: 1-508-893-0176
E-mail: support@hoeferinc.com
Web: www.hoeferinc.com
•
p14
7. Disposal
This equipment is marked with the crossed out
wheeled bin symbol to indicate that it must not
be disposed of with unsorted waste.
Instead it is your responsibility to dispose of
your equipment correctly at life cycle end by
handing it over to an authorized facility for
separate collection and recycling.
It is also your responsibility to decontaminate
the equipment in case of biological, chemical
and/or radiological contamination, so that the
persons involved in the disposal and recycling
of the equipment are protected from any
potential hazard.
For more information about where you can
drop off your waste equipment, please contact
your local dealer from whom the equipment was
purchased originally.
By doing so, you will help to conserve natural
and environmental resources and you will ensure
that your equipment is recycled in a manner that
protects human health.
Thank you.
•
p15
8. Warranty
Hoefer, Inc. warrants that the unit you have
received has been thoroughly tested and meets
its published specification.
This unit (excluding all accessories) is warranted
against defective material and workmanship for
a period of twelve (12) months from the date of
shipment ex factory.
Hoefer, Inc. will repair all defective equipment
returned during the warranty period without
charge, provided the equipment has been used
under normal laboratory conditions and in
accordance with the operating limitations and
maintenance procedures outlined in this instruction manual and when not having been subject
to accident, alteration, misuse or abuse.
No liability is accepted for loss or damage arising from the incorrect use of this unit. Hoefer,
Inc.’s liability is limited to the repair or replacement of the unit or refund of the purchase price,
at Hoefer, Inc.’s option. Hoefer, Inc. is not liable
for any consequential damages.
Hoefer, Inc. reserves the right to alter the specification of its products without prior notice. This
will enable us to implement developments as
soon as they arise.
Hoefer, Inc. products are for research use only.
A return authorization must be obtained from
Hoefer, Inc. before returning any product for
warranty repair on a freight-prepaid basis.
•
p16
8.1 Warning
• Do not attempt to remove the outer casing
or make repairs to our electrical range of
products, should any unit fail.
• Contact Hoefer, Inc. immediately if the need
for repair or servicing should arise.
8.2 Safety Precautions
• Should the sensor develop a fault or become
disconnected the fan will remain “ON” so
safeguarding against gel overheating.
• The fan utilizes a brushless motor with reverse
polarity protection.
• A 500 mAmp 240 Volt antisurge fuse is
located on the rear panel in a fuse holder
immediately below the mains supply socket.
•
p17
9. Ordering Information
Number Thickness Well width Well depth Well volume µl
Product of wells (mm) (mm) (mm) per 1 mm depth
Shark’s Tooth Combs
SQ33-C35-48K 48 0.35 3 10 5
SQ33-C35-60KMC 60* 0.35 2.5 10 4
SQ33-C35-96K 96 0.35 2.5 9 3.5
Standard Combs
SQ33-C35-48 48 0.35 4 5 7
SQ33-C35-60MC 60* 0.35 2 5 3.5
*Microtiter spacing for use with a multichannel pipette.
Additional Accessories and Replacement Parts Catalog number
Glass Plates, Rectangular, 33 × 41 cm (2 pieces) SQ33-GPLT
Glass Plates, Notched, 33 × 41 cm (2 pieces) SQ33-NGPLT
Spacers, 0.35 mm thick (2 pieces) SQ33-SP
Fan Sensor Kit, 115 VAC SQ33-HS-115V
Fan Sensor Kit, 230 VAC SQ33-HS-230V
•
p18
Hoefer, Inc.
84 October Hill Road
Holliston, MA 01746
Toll Free: 1-800-227-4750
Phone: 1-508-893-8999
Fax: 1-508-893-0176
E-mail: support@hoeferinc.com
Web: www.hoeferinc.com
Hoefer is a registered trademark
of Hoefer, Inc.
© 2013 Hoefer, Inc. —
All rights reserved.
Printed in the USA.
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