Hoefer SG500 User Manual
user manual
Hoefer SG500
Gradient maker
um SG500-IM /Rev.E0/08-12
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SG500 instructions ...............................................1
1. Generating linear gradients .................................2
Pouring gradients from the top ..........................4
Pouring gradients from the bottom ......................5
2. Generating convex and concave exponential
gradients ..........................................................6
To generate an exponential gradient ....................7
3. Care and maintenance .......................................9
4. Ordering information ..........................................9
pi
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push-pull valve
reservoir (back) chamber
mixing (front) chamber
connector channel
outlet connector
(4 mm ID tubing)
slide stop
Fig 1. Hoefer SG500
gradient maker.
Included but not shown:
Hose clamps (4)
SG500 instructions
The Hoefer® SG500 gradient maker is designed
for producing linear gradients of aqueous
solutions ranging in volume from 100–500 ml.
The SG500 can be used to create convex and
concave exponential gradients with the addition
of a one-holed rubber stopper, a piece of rigid
tubing and a piece of flexible tubing. The unit
is fabricated from cast acrylic plastic sheet and
tubing. It is suitable for casting acrylamide pore
gradient gels and delivering salt gradients for
low pressure chromatography systems.
p1
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1. Generating linear gradients
open
closed
To generate a linear gradient between two
concentrations, equal volumes of solutions of the
two concentrations are measured into the two
chambers of the gradient maker. As solution is
delivered out of the mixing chamber, an equal
volume flows in from the reservoir chamber
where it is rapidly diluted and mixed to uniformity by a magnetic stir bar. The initial concentration delivered will be that of the solution in
the mixing chamber, the final concentration will
be that of the reservoir chamber. For the most
consistent delivery of gradients, a peristaltic
pump is recommended.
1
Make sure all parts are clean and liquid flows freely
through all the connecting channel of the slide valve
and pump and delivery tubing.
2
Add a magnetic stir bar of the appropriate dimensions
(20–30 mm long) to the mixing chamber and place
the unit on a magnetic stirrer. If volumes will be less
than half the capacity of the unit, an identical stir bar
should be placed in the reservoir chamber as well to
balance the displacement and prevent backflow into
the reservoir when the chambers are first connected.
Connect tubing to the outlet connector and pump,
and adjust pump speed, if used. Position or connect
the tubing to the receiving vessel (gel casting unit,
column, etc.).
Fig 2. Open and closed positions
of slide valve.
p2
•
3
Close the slide valve (out on side of white slide stop
button, Fig 2). If the outlet tubing is not controlled
by a pump, clamp it off near the gradient former.
Add the required volume of the final solution to the
reservoir (back) chamber.
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