Hoefer SG30, SG50, SG100 User Manual

user manual

um  SG100-IM/Rev.C0/08-12

Hoefer SG15, SG30,
SG50 and SG100

Gradient makers

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1. Introduction: Hoefer SG15, SG30, SG50,

and SG100 gradient makers ............................... 1

2. Generating linear gradients .................................2

Pouring gradients from the top ..........................4

Pouring gradients from the bottom ......................5

3. Care and maintenance ....................................... 6

4. Ordering information ..........................................6

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1. Introduction: Hoefer SG15,

SG30, SG50, and SG100
gradient makers

The Hoefer® SG gradient makers are designed
for producing linear gradients of aqueous solu­tions ranging in volume from 15–100 ml. The
gradient makers are machined from solid blocks
of acrylic plastic. They are suitable for casting
acrylamide pore gradient gels, casting immobi­lized pH gradient gels, pouring density gradients
for centrifugal sedimentation separations and
delivering salt gradients for low pressure chro­matography systems.

The gradient makers have a white Luer outlet
fitting that can take either tubing with female

Fig 1. Hoefer SG gradient maker.

Included but not shown:

Adaptor barbed fitting (1)
22-gauge needle (1)

reservoir (black)
chamber

mixing (front)
chamber

support
rod

stopcock
nut

stopcock
washer

white Luer outlet
connector (4 mm
id tubing)

delivery
stock

connector
channel

connector
stopcock