Hoefer PR150 User Manual

user manual
Hoefer PR150
Incubation manifold
umPR150-IM /Rev.E0/08-12
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Fig 1. Components of the PR150.
sample gasket
gasket seating lip

Introduction

Transfer of proteins or nucleic acids, fraction­ated by gel electrophoresis, to an immobilizing membrane surface increases the sensitivity of a wide range of detection methods, reduces time of analysis and makes sequential probing possible. In particular it has made possible use of immunological procedures that are not practi­cal to carry out in a gel. To assay material on a sheet of membrane with different probes simul­taneously commonly requires first cutting the membrane into a number of parallel strips. This procedure however destroys the correspondence between different lanes or positions on a gel.
Pressure gasket
gasket seating lip
sealing ridge
sample chamber
Pressure plateSample plate
p1
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