Hoefer IEF100 User Manual

user manual

Hoefer IEF100

Isoelectric Focusing Unit

um IEF100-IM/Rev.B0/07-12

Page Finder

Important Information ................................................................ii

Waste Electrical And Electronic Equipment (WEEE) .................... vi

Function and Description ...........................................................1

Speciﬁcations ...........................................................................2

System Components .................................................................3

Component Descriptions .............................................................5

Programming the IEF100 .........................................................10

IEF100 Operation ....................................................................20

Optional Data Connections .......................................................35

Care and Maintenance ............................................................42

Technical Service and Repair ...................................................42

Troubleshooting .......................................................................43

Ordering Information ...............................................................45

Appendix A: Preprogrammed Protocols ......................................46

Appendix B: Reagents and Solutions .........................................51

Appendix C: References IEF100 ...............................................57

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Important Information – English

• If this equipment is used in a manner not specied by Hoefer, Inc. the protection provided by the equipment may be impaired.

• This instrument is designed for indoor laboratory use only.

• Only accessories and parts approved or supplied by Hoefer, Inc. may be used for operating, maintaining, and servicing this product.

• Only use a power supply that is CE marked or safety certied by a nationally recognized testing laboratory.

• The safety lid must be in place before connecting the power supply leads to a power supply.

• Turn all power supply controls o and disconnect the power leads before removing the safety lid.

• Circulate only water or 50/50 water/ethylene glycol through the heat exchanger if so equipped. Do not connect the heat exchanger to a water tap or any coolant source where the water pressure is unregulated.

• Never introduce antifreeze or any organic solvent into any part of the instrument. Organic solvents will cause irreparable damage to the unit!

• Do not operate with buer temperatures above the maximum specied technical specications. Overheating will cause irreparable damage to the unit!

Duležité Informace – Czech

• Pokud by toto zařízení je použito způsobem, který není podle Hoefer, Inc. ochrana poskytovaná na základě zařízení může být narušena.

• Tento nástroj je určen pro vnitřní použití v laboratoři pouze.

• Pouze příslušenství a části schválen, nebo poskytnutých Hoefer, Inc. mohou být použity pro provoz, údržbu, a údržbě tohoto výrobku.

• zdroj napájení používají jen že je opatřen označením CE osvědčena nebo bezpečnost vnitrostátně uznanými zkušebními laboratoř.

• Bezpečnosti lid musí být zavedena před připojením napájecí zdroj napájení vede k.

• Turn veškeré napájení kontroly vypnuto a odpojit před odběrem energie vede bezpečnostní víko.

• Rozeslat pouze voda nebo 50/50 voda/ethylenglykolu prostřednictvím výměník tepla je li to vybavena. Nemají připojení výměník tepla s vodními setřepná nebo jakékoli chladicí kapaliny zdroje, kde tlak vody je neregulo.

• Nikdy zavést prostředek proti zamrznutí nebo jakákoli organická rozpouštědla do jakékoli části z tohoto nástroje. Rozpustidlům způsobí nenapravitelné poškození jednotka!

• Nejsou provozována s pufru teplotách nad maximální stanovenou technickými specikacemi. Přehřátí způsobí nenapravitelné poškození jednotka!

Vigtig Information – Danish

• Hvis dette udstyr bruges i en måde ikke speciceret ved Hoefer, Inc. den beskyttelse, som er blevet forsynet af udstyret kan måske svækkes.

• Dette instrument er designet for indendørs laboratoriumbrug bare.

• Bare tilbehør og del godkendede eller forsynede ved Hoefer, Inc. kan måske bruges for drive, funktionsfejl, og betjening dette produkt.

• bruger Bare en strømforsyning, der er CE markerede eller sikkerhed, som er blevet attesteret af en, som nationalt er blevet anerkendt prøve laboratorium.

• Sikkerhedlåget må være på plads før forbinding strømforsyningsblyet til en strømforsyning.

• Drejer alle strømforsyningskontroller af og afbryder kraftblyet før erning sikkerhedlåget.

• Cirkulerer bare vand eller 50/50 vand/ethylene glykol gennem varmeveksleren i så fald udrustet. Forbind ikke varmeveksleren til en vandhane eller nogen kølemiddelkilde hvor vandtrykket er unregulated.

• Introducerer Aldrig antifreeze eller noget organisk opløsningsmiddel ind i nogen del af instrumentet. Organiske opløsningsmidler vil forårsage uboelig skade til enheden!

• Driver ikke med stødpudetemperaturer over maksimummet specicerede tekniske specications. Overheding vil forårsage uboelig skade til enheden!

Belangrijke Informatie – Dutch

• Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer, Inc. is gespeciceerd de bescherming die door de uitrusting is verzorgd kan worden geschaad.

• Dit instrument is voor binnenlaboratoriumgebruik enkel ontworpen.

• Enkel onderdelen en delen keurden goed of leverden door Hoefer, Inc. kan voor het bedienen worden gebruikt, handhavend en onderhouden van dit product.

• gebruik Enkel een netvoeding die CE is markeerde of veiligheid die door een is gecerticeerd die nationaal is herkend testene laboratorium.

• Het veiligheidsdeksel moet in plaats voor het verbinden van de netvoeding leidt tot een netvoeding zijn.

• Doe alle netvoedingscontroles Uit en koppel los de machtleiding voor het verwijderen van het veiligheidsdeksel.

• Circuleer enkel water of 50/50 water/ethyleenglycol door de hitte exchanger zo ja uitrust. Verbind de hitte exchanger naar een waterkraan of koelmiddelbron niet waar de waterdruk niet geregulariseerd is.

• Stel Nooit antivriesmiddel of organische oplosmiddelen in deel van het instrument voor. Organische oplosmiddelen zullen onherstelbare schade aan de eenheid veroorzaken!

• Bedien niet met buertemperaturen boven het maximum speciceerde technische specicaties. Oververhittend zal onherstelbare schade aan de eenheid veroorzaken!

Tärkeää Tietoa – Finnish

• Jos tätä varusteita käytetään tavassa ei määritetty Hoefer, Inc. suojelu ehkäisty varusteille saattaa olla avuton.

• Tämä väline suunnitellaan sisälaboratoriokäytölle vain.

• Vain lisävarusteet ja osat hyväksyivät tai toimitti Hoefer, Inc. oheen ää voi käyttää käyttämiselle, valvoalle, ja servicing tämä tuote.

• Vain käyttää käyttöjännitettä joka on CE merkitsi tai turvallisuus joka on todistanut aidoksi ohi joka on kansallisesti tunnustettnut testaaminen laboratoriota.

• Turvallisuuskansi täytyy olla paikallaan ennen yhdistäminen

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pii

käyttöjännitelyijyjä käyttöjännitteeseen.

• Kiertää kaikki käyttöjännitevalvonnat ja irrottaa valtalyijyt ennen poistaminen turvallisuuskantta.

• Kiertää vain vesi tai 50/50 vesi/ethyleneä glycol siinä tapauksessa varustetun lämmönvaihtimen läpi. Älä yhdistä lämmönvaihdinta vesinapautukseen eikä jäähdytysnestelähteeseen, missä vesipaine on unregulated.

• Pakkasneste eikä orgaaninen liuotin välineen osassa ei esitele Koskaan. Orgaaniset liuottimet aiheuttavat korvaamattoman vahingon yksikköön!

• Ei käytä puskuria yllä olevia lämpötiloja enintään määritetyillä teknisillä täsmennyksillä. Ylikuumeneminen aiheuttaa korvaamattoman vahingon yksikköön!

Information Importante – French

• Si cet équipement est utilisé dans une manière pas spécié par Hoefer, Inc. la protection fourni par l’équipement pourrait être diminuée.

• Cet instrument est conçu pour l’usage de laboratoire intérieur seulement.

• Seulement les accessoires et les parties ont approuvé ou ont fourni par Hoefer, Inc. pourrait être utilisé pour fonctionner, maintenir, et entretenir ce produit.

• utilise Seulement une alimentation qui est CET a marqué ou la sécurité certié par un nationalement reconnu essayant le laboratoire.

• Le couvercle de sécurité doit être à sa place avant connecter l’alimentation mene à une alimentation.

• Tourner tous contrôles d’alimentation de et débrancher les avances de pouvoir avant enlever le couvercle de sécurité.

• Circuler seulement de l’eau ou 50/50 glycol d’eau/éthylène par l’exchanger de chaleur si si équipé. Ne pas connecter l’exchanger de chaleur à un robinet d’eau ou à la source d’agent de refroidissement où la pression d’eau est non régulée.

• Ne Jamais introduire d’antigel ou du dissolvant organique dans n’importe quelle partie de l’instrument. Les dissolvants organiques causeront des dommages irréparables à l’unité!

• Ne pas fonctionner avec les températures de tampon au-dessus du maximum a spécié des spécications techniques. La surchaue causera des dommages irréparables à l’unité !

Wichtige Informationen – German

• Wenn diese Ausrüstung gewissermaßen nicht angegeben durch Hoefer, Inc. verwendet wird, kann der durch die Ausrüstung zur Verfügung gestellte Schutz verschlechtert werden.

• Dieses Instrument wird für den Innenlaborgebrauch nur dafür entworfen.

• Nur Zusätze und Teile genehmigten oder lieferten durch Hoefer, Inc. kann für das Funktionieren, das Aufrechterhalten, und die Wartung dieses Produktes verwendet werden.

• Verwenden Sie nur eine Energieversorgung, die CE gekennzeichnet oder durch ein national anerkanntes Probelaboratorium bescheinigte Sicherheit ist.

• Der Sicherheitsdeckel muss im Platz vor dem Anschließen der Energieversorgung sein führt zu einer Energieversorgung.

• Alle Energieversorgungssteuerungen abdrehen und die Macht trennen führt vor dem Entfernen des Sicherheitsdeckels.

• Nur Wasser oder 50/50 Glykol des Wassers/Äthylens durch den Wärmeaustauscher, wenn so ausgestattet, in Umlauf setzen. Verbinden Sie den Wärmeaustauscher mit einem Wasserklaps oder jeder Kühlmittel-Quelle nicht, wo der Wasserdruck ungeregelt wird.

• Führen Sie nie Frostschutzmittel oder jedes organische Lösungsmittel in jeden Teil des Instrumentes ein. Organische Lösungsmittel werden nicht wiedergutzumachenden Schaden der Einheit verursachen!

• Mit Puertemperaturen über angegebenen technischen Spezizierungen des Maximums nicht funktionieren. Die Überhitzung wird nicht wiedergutzumachenden Schaden der Einheit verursachen!

Informazioni Importanti – Italian

• Se quest’apparecchiatura è usata in un modo specicato da Hoefer, Inc. la protezione fornito dall’apparecchiatura potrebbe essere indebolita.

• Questo strumento è disegnato per l’uso di laboratorio interno solo.

• Solo gli accessori e le parti hanno approvato o hanno fornito da Hoefer, Inc. potrebbe essere usato per operare, per mantenere, e per revisionare questo prodotto.

• usa Solo un alimentatore che è CE ha marcato o la sicurezza certicato da un nazionalmente riconosciuto testando il laboratorio.

• Il coperchio di sicurezza deve essere nel luogo prima di collegare i piombi di alimentatore a un alimentatore.

• Spegne tutto i controlli di alimentatore e disinserisce i piombi di potere prima di togliere il coperchio di sicurezza.

• Circola solo l’acqua o 50/50 glicole di acqua/etilene attraverso lo scambiatore di calore se così equipaggiato. Non collegare lo scambiatore di calore a un rubinetto di acqua o qualunque fonte di refrigerante dove la pressione di acqua è sregolata.

• Non introduce mai l’antigelo o qualunque solvente organico in qualunque parte dello strumento. I solventi organici causeranno il danno irreparabile all’unità!

• Non opera con le temperature di tampone al di sopra del massimo ha specicato le descrizioni tecniche. Il surriscaldamento causerà il danno irreparabile all’unità!

Viktig Informasjon – Norwegian

• Hvis dette utstyret blir brukt i en måte ikke spesisert ved Hoefer, Inc. beskyttelsen som ha blitt git av utstyret kan bli svekket.

• Dette instrumentet er utformet for innendørs laboratoriumbruk bare.

• Bare tilbehør og deler godkjente eller forsynte ved Hoefer, Inc. kan bli brukt for drive, vedlikeholde, og betjene dette produktet.

• bruker Bare en kraftforsyning som er CE merket eller sikkerhet som ha blitt sertisert av et som nasjonalt ha blitt anerkjent prøver laboratorium.

• Sikkerheten lokket må være på plass før forbinding kraftforsyningene blyene til en kraftforsyning.

• Vender all kraftforsyningsstyring av og frakopler kreftene blyene før erning sikkerheten lokket.

• Sirkulerer bare vann eller 50/50 vann/ethylene glykol gjennom oppvarmingen veksleren i så fall utstyrer. Ikke forbind oppvarmingen veksleren til en vanntapp eller noe kjølemiddelkilde hvor vannet

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trykket er unregulated.

• Introduserer Aldri antifreeze eller noe organisk løsemiddel inn i noe del av instrumentet. Organiske løsemiddler vil forårsake irreparabel skade på enheten !

• Driver med buertemperaturer over maksimum ikke spesiserte teknisk spesikasjoner. Å overoppheting vil forårsake irreparabel skade på enheten !

Wazne Informacje – Polish

• Jeżeli ten sprzęt jest wykorzystywany w sposób nie określone przez Hoefer, Inc. do ochrony przewidzianej przez urządzenie może zostać obniżony.

• Instrument ten jest przeznaczony do użytku w laboratoriach kryty tylko.

• Tylko akcesoriów i części zatwierdzone lub dostarczone przez Hoefer, Inc. mogą być wykorzystane do eksploatacji, utrzymania i obsługi tego produktu.

• korzystać jedynie zasilacza że jest noszące oznakowanie CE lub bezpieczeństwa uwierzytelnione przez uznane na poziomie krajowym laboratorium badawcze.

• Bezpieczeństwo lid musi być w miejsce przed podłączeniem zasilania prowadzi do zasilania.

• Zaś wszystkie źródła zasilania urządzenia sterujące o i odłączyć moc prowadzi przed odbiorem bezpieczeństwa lid.

• Krążą tylko wody lub wody 50/50/ethylene glycol wymiennik ciepła poprzez jeśli tak wyposażone. Nie należy połączyć wymiennik ciepła woda z kranu lub jakimkolwiek chłodziwo źródła, jeżeli ciśnienie wody jest nieuregulowanych.

• Nigdy nie wprowadzać rozpuszczalnika organicznego przeciw zamarzaniu lub jakichkolwiek na dowolną część dokumentu. Rozpuszczalniki organiczne spowoduje nieodwracalne szkody dla jednostki!

• Nie działają w buforze temperatury powyżej maksymalnego określone specykacje techniczne. Przegrzania spowoduje nieodwracalne szkody dla jednostki!

Informações Importantes – Portuguese

• Se este equipamento é usado numa maneira não especicada por Hoefer, Inc. que a protecção fornecida pelo equipamento pode ser comprometida.

• Este instrumento é projectado para uso de interior de laboratório só.

• Só acessórios e partes aprovaram ou forneceu por Hoefer, Inc. pode ser usada para operar, manter, e servicing este produto.

• Só usa um estoque de poder que é CE marcou ou segurança registrada por um nacionalmente reconhecido testando laboratório.

• A tampa de segurança deve estar em lugar antes de ligar o estoque de poder leva a um estoque de poder.

• Desliga todos controlos de estoque de poder e desconecta os chumbos de poder antes de retirar a tampa de segurança.

• Circulam só água ou 50/50 glicol de água/ethylene pelo exchanger de calor se for assim equiparam. Não ligue o exchanger de calor a uma torneira de água nem qualquer fonte de refrigerante onde a pressão de água é não regulado.

• Nunca introduz anticongelante nem qualquer orgânico solvente em

qualquer parte do instrumento. Orgânico solvente causará agressão irreparável à unidade!

• Não opera com temperaturas de buer acima do máximo especicou especicações técnicas. Superaquecer causará agressão irreparável à unidade!

Información Importante – Spanish

• Si este equipo es utilizado en una manera no especicado por Hoefer, Inc. la protección proporcionado por el equipo puede ser dañada.

• Este instrumento es diseñado para el uso interior del laboratorio sólo.

• Sólo accesorios y partes aprobaron o suministraron por Hoefer, Inc. puede ser utilizado para operar, para mantener, y para atender a este producto.

• Sólo utiliza una alimentación que es CE marcó o la seguridad certicada por un nacionalmente reconocido probando el laboratorio.

• La tapa de la seguridad debe estar en el lugar antes de conectar la alimentación lleva a una alimentación.

• Apaga todos controles de alimentación y desconecta los plomos del poder antes de quitar la tapa de la seguridad.

• Circula sólo agua o 50/50 glicol de agua/etileno por el intercambiador de calor si ése es el caso equiparon. No conecte el intercambiador de calor a un toque de la agua ni cualquier fuente del líquido refrigerante donde la presión del agua está libre.

• Nunca introduce anticongelante ni algún solvente orgánico en cualquier parte del instrumento. Los solventes orgánicos causarán daño irreparable a la unidad!

• No opera con temperaturas de búfer encima del máximo especicó especicaciones técnicas. Recalentar causará daño irreparable a la unidad!

Viktig Information – Swedish

• om denna utrustning används i ett sätt som inte har speciceras av Hoefer, Inc. skyddet tillhandahöll vid utrustningen kan skadas.

• Detta instrument formges för inomhuslaboratorium användning bara.

• Bara medhjälpare och delar godkände eller levererade vid Hoefer, Inc. kan användas för fungera, underhålla, och servicing denna produkt.

• använder bara en kraft tillgång som är CE markerade eller säkerhet intygade vid en nationellt erkänd testande laboratorium.

• Säkerheten locket måste vara på platsen före koppla kraften tillgången blyen till en kraft tillgång.

• Vänder sig alla kraft tillgång kontroller av och kopplar bort kraften blyen före ytta säkerheten locket.

• Cirkulerar bara vatten eller 50/50 vatten/ethylene glycol genom värmen exchanger i så utrustad fall. Inte kopplar värmen exchanger till en vatten kran eller något kylmedel källa där vattnet trycket är unregulated.

• Inför aldrig kylvätska eller något organiska lösningsmedel in i någon del av instrumentet. Organiskt lösningsmedel ska orsaka irreparable skada till enheten!

• Använd inte med buert temperaturer över det högsta angivna tekniska specikationerna. Överhettning skulle orsaka irreparabla skador på enheten!

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English

Waste Electrical And Electronic Equipment (WEEE)

This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment.

French

German

Italian

Ce symbole indique que les déchets relatifs à l’équipement électrique et électronique ne doivent pas être jetés comme les ordures ménagères non-triées et doivent être collectés séparément. Contactez un représentant agréé du fabricant pour obtenir des informations sur la mise au rebut de votre équipement.

Dieses Symbol kennzeichnet elektrische und elektronische Geräte, die nicht mit dem gewöhnlichen, unsortierten Hausmüll entsorgt werden dürfen, sondern separat behandelt werden müssen. Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf, um Informationen hinsichtlich der Entsorgung Ihres Gerätes zu erhalten.

Questo simbolo indica che i riﬁuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come riﬁuti municipali indifferenziati e devono invece essere raccolti separatamente. Per informazioni relative alle modalità di smantellamento delle apparecchiature fuori uso, contattare un rappresentante autorizzato del fabbricante.

Spanish

Swedish

Este símbolo indica que el equipo eléctrico y electrónico no debe tirarse con los desechos domésticos y debe tratarse por separado. Contacte con el representante local del fabricante para obtener más información sobre la forma de desechar el equipo.

Denna symbol anger att elektriska och elektroniska utrustningar inte får avyttras som osorterat hushållsavfall och måste samlas in separat. Var god kontakta en auktoriserad tillverkarrepresentant för information angående avyttring av utrustningen.

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Note: Ohm’s law states that the 10 mA of output is available at 1000 V and less.

Function and Description

The Hoefer IEF100 Isoelectric Focusing instrument is designed to perform isoelectric focusing of proteins in Immobilized pH Gradient (IPG) strips. It is an integrated system with a built-in Peltier cooled temperature platform, and a 12,000 V, 10 W power supply capable of delivering up to 10 mA. There are 30 fully editable protocols, nine of which are pre-programmed with recommended IEF protocols. One running tray will hold all currently available IPG strips, 3.0 – 3.5 mm wide. The IPG strips are run gel side up, using electrode wicks to help remove impurities that collect at the end of the pH gradients. Samples can be loaded into the IPG strips during rehydration, or with sample cups.

The IEF100 is fully tested and certiﬁed to all applicable international standards.

Features of the IEF100 are:

• One 6 channel tray that focuses: Up to 6, 7 – 24 cm IPG strips using the single electrode set. Up to 12, 7 cm IPG strips using the dual electrode accessory (included).

Fig. 1. The Hoefer IEF100.

• Sample loading during rehydration or with sample cup.

• Monitors current in each IPG strip.

• Integrated 12,000 volt DC power supply, with 10 mA capability, the most voltage and current capability available.

• A large blue/white LCD display for easy viewing that supports a graphical display of voltage and current.

• The ability to program, edit and store up to 30 protocols, each with 9 steps.

• Constant power mode.

• A real time clock, settable to local time zones.

• Temperature controlled platform.

• Ethernet and RS232 ports to report data or load protocols.

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This declaration of conformity is only valid for the instrument when it is:

• used in laboratory locations,

• used as delivered from Hoefer, Inc. except for alterations described in the user manual, and

• connected to other CE-labeled instruments or products recommended or approved by Hoefer, Inc.

Specifications

Capacity using Single Electrode set 1– 6 IPG strips 7 to 24 cm long

Capacity using Dual Electrode accessory 2– 12 IPG strips 7 cm long

Output Volt 12,000 V Current 10 mA Power 10 W

Power in 100 – 240 V

50 – 60 Hz 100 W

User interface Large blue/white graphical display Knob 7 button keypad

Platform temperature 15 – 25 °C

Protocol capacity 30 programs with 9 steps each

Input/output ports Ethernet, RS232

Dimension (w × d × h) 38 cm × 27 cm × 19 cm

Weight 8 kg

Environmental Indoor use 4 – 40 °C operating conditions Humidity up to 80% Altitude up to 2000 m Installation category II Pollution degree 2

Product certiﬁcations EN61010-1:2001, EN61326:1998, CE, WEEE, RoHS

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Fig. 2. The IEF100 unit.

System Components

Unpacking

Unwrap all packages carefully and compare the contents with the packing list, making sure all items arrived. If any part is missing, contact your local Hoefer, Inc. sales ofﬁce. Inspect all components for damage that may have occurred while the unit was in transit. If any part appears damaged, contact the carrier immediately. Be sure to keep all packing material for damage claims or to use should it become necessary to return the unit.

Required but not supplied:

• Immobilized pH Gradient (IPG strips).

• Reagents required for sample preparation and strip rehydration.

• Mineral oil.

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Fig. 3. Instrument components.

Electrode Wicks IEF106

Sample Cups IEF108

Large Rehydration/ Equilibration Tray IEF110

Small Rehydration/ Equilibration Tray IEF109

Focus Tray IEF105

Accessory Box

Level

Forceps

(–) Electrode

Brush

115 V Power Cord

(+) Electrode

Not pictured: Dual Electrode Accessory IEF212.

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230 V Power Cords (EU and UK)

Note: Turn the knob slowly to get desired movement on the screen. Turning the knob too fast causes the cursor to blink, and stay in place. If this happens, turn the knob slower until the proper behavior is observed.

Note: The click action of the knob is to quickly push and release, like a mouse click. Do not hold the knob in.

Component Descriptions

Safety Lid

The transparent safety lid must be closed in order for voltage to be applied to the electrodes. Interlocks are used to stop the voltage output should the lid be opened during electrophoresis.

Knob

The knob moves the cursor or changes the values of a given ﬁeld. Pressing the knob inwards to “click” switches between these two functions. A LED indicates which function is active, MOVE or SET.

Horizontal Arrow Keys

The horizontal arrows are used when setting protocol names, and the date and time ﬁelds.

High Volt Indicator LED

This LED is lit when voltage is being applied to the electrodes.

Fig. 4. Front of the instrument.

RS232 port

Safety lid

Knob

Horizontal Arrow Keys

High Voltage LED

STOP Button

LCD Display

Function Keys Ethernet Port

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STOP Button

Immediately stops the IEF, and terminates the protocol.

LCD Display

The large blue/white display simpliﬁes the interface, and displays graphical IEF results.

Function Keys

The function of these keys is shown on the display directly above the key. The function varies depending on what screen is active.

Ethernet Port/RS232 Port

These two ports can be used to download IEF data from the instrument and transfer protocols.

Air Flow

Fans pull air in through the side vents, and blow air out the rear vents. The air cools the electronic components, and helps the Peltier modules maintain constant temperature.

Mains Power

The power entry module operates at all volts and frequencies.

Fig. 5. Rear panel.

Note: The vents should not be blocked.

Leveling Feet/Bubble Level

The leveling feet and the bubble level can be used to level the IEF platform. A level instrument will help ensure oil completely covers the IPG strips.

Leveling Feet

Air Out Vents

Air In Vents (2X)

Power Entry Module ON/OFF Switch

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Focusing Tray

The 6 channel focusing tray accommodates IPG strips up to 24 cm in length. Small grooves in the tray allow for easier removal of the IPG strip after focusing. Detail of these features can be seen on page 24.

The channels in the tray are numbered 1– 6, from front to back of the IEF100.

The tray ﬁts in the instrument in only one orientation. It is set in place on the right side of the platform, and moved sidewise to the left, clamping the tray down against the cold plate. The clamping mechanism improves the heat transfer for the plastic trays.

There are (+) and (–) marks on the tray and the instrument to help with proper orientation.

Fig. 6. Top of the instrument.

(+) Electrode

Sample Cups

Align IEF Strips with this Line

(–) Electrode

Focusing Tray

Clamps

Clamping Tabs (6X)

+ –

Front of Instrument

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Fig. 7. Positive electrode (top), and negative electrode (bottom).

Electrodes

Positive Electrode (Anode)

A high volt connector attaches the positive (+) electrode(s) to the high volt terminal. The anode has a single white wire and connector, and locates on the left side of the tray.

Negative Electrode (Cathode)

A six wire (lan) connector attaches the negative (–) electrode(s) to the ground terminal, monitoring the current in each lane. The cathode locates on the right side of the tray.

Electrodes should be placed in the tray with the number “1” towards the front of the tray, and the platinum wire electrodes facing the middle of the IPG strips.

(–) Negative Electrode

(+) Positive Electrode

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Note: The electrodes will not make contact with the IPG strips without the electrode wicks in place.

Electrode Wicks

The electrode wicks are small rectangular ﬁlter papers that are placed over the end of the IPG strips. The electrodes make contact with the top of the wicks. The electrode wicks help remove impurities that collect at the end of the IPG strips, and generally improve electrode contact.

The electrode wicks come in long strips, and must be cut apart into individual rectangles as they are used. The electrode wicks need to be moistened by lightly blotting with water prior to use.

Sample Cups

Note: The sample cups can be cut into

individual cups, if desired.

Fig. 8. Sample cups.

Fig. 9. Rehydration/equilibration trays.

The sample cups load protein into the IPG strip. Sample cups can hold up to 240 μl of sample. The cups can be applied in most places along the length of the IPG strip, and are normally applied at the anode (+) end.

Rehydration/Equilibration Trays

The rehydration/equilibration trays can be used for both functions. The lanes are narrow enough for proper rehydration, but deep enough to hold the volume of reagent required for equilibration. A small tray is included for use with 7 cm IPG strips. The large tray can be used with IPG strips up to 24 cm in length.

Brush

The cleaning brush is used for cleaning the focusing tray.

Forceps

Forceps facilitate handling of IPG strips.

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Programming the IEF100

Main Screen

When turned ON, the main screen is displayed (Fig. 10). The main screen contains the following ﬁelds.

Date and Time

The date and time are set up at Paciﬁc Standard Time. Use the OPTION button to set the date and time for your location. (See options screen page 17).

List of 30 Protocols

The ﬁrst nine protocols are shown on the display. One of the protocols is always highlighted. Use the knob to scroll through the other protocols.

The instrument is preloaded with 9 protocols for focusing 7, 18 or 24 cm IPG’s. These are general guidelines for focusing. IPG strip manufacturers normally give recommended focusing times in total volt-hours (volts multiplied by hours). Both under and over focusing can be problematic. Optimum focusing times should be determined by the end user considering the IPG strip, the sample type and sample protein load. All protocols can be overwritten, if desired.

Fig. 10. Main screen.

In addition to these 9 protocols, there are 21 additional unnamed protocols without preprogrammed steps, allowing the user ample space to create custom protocols.

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Note: The last run data is retained in memory until a new run is started. Once a new run is started, the last run data is no longer available.

Note: The volt and current graphs can not be printed directly from the IEF100. If data output is sent to a computer, the data can be transferred to a program such as Excel to create the graph.

The Four Buttons: EDIT, RUN, OPTION and GRAPH

EDIT

Edits the highlighted protocol.

RUN

Starts the highlighted protocol.

OPTION

Allows access to port settings and the date and time.

GRAPH

Will display the volt and measured currents graphically. Use the knob to scroll through the voltage and the μA proﬁles in channels 1– 6. The active run will be displayed.

If not actively running a protocol, the graph button will display the last run data.

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Fig. 11. Edit protocol screen. All parameters for an entire protocol are viewable on one screen.

Edit Protocol Screen

From the main menu, use the knob to highlight the desired protocol. Press the button beneath EDIT on the screen. The IEF100 will now display the edit protocol screen (Fig. 11).

All IEF parameters are displayed on one screen. Each protocol can be programmed with up to nine steps. The protocol ends with the ﬁrst step that has a step time of zero.

Use the knob to move between the following editable ﬁelds. Once highlighted, click the knob to change the value of the ﬁeld.

Protocol Number

Click the knob. The knob will now cycle through the 30 protocols, allowing a quick review of each protocol.

Protocol Name

Protocol names can have up to 16 alphanumeric symbols. The following symbols are available:

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z % . @ / - + m u n 0 1 2 3 4 5 6 7 8 9

Highlight the protocol name ﬁeld, and click the knob.

A cursor will cause the ﬁrst character to blink. Turn the knob to scroll through the above symbols. Once the desired symbol is reached, use the horizontal arrows (the keys directly beneath the knob) to move the cursor to the next position. Repeat until the protocol name is correct.

The “blank space” is between the lower case “n” and the “0” symbols.

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The lower case “m”, “u”, and “n” can be used to designate milli, micro, and nano, if desired.

When the name is correct, click the knob to enter the name, and move to the next ﬁeld.

Delay

The delay line is an optional step. Both a time and a temperature can be entered. Focusing will start after the delay time has elapsed. This can be helpful for overnight runs where the user may want to have focusing end at some speciﬁc time the next morning. The Peltier modules will maintain the cold plate at the set temperature.

Note: Times to the nearest minute increment can be uploaded to protocols from a computer.

Note: It is recommended not to exceed 6000 V or more than 0.5 W/ strip for a 7 cm IPG. Please follow the IPG strip manufacturer’s instructions for recommended limits.

Parameter Units Range Increment

Time (hour:min) 0:00 – 99:59 0:15

Temperature (°C) 15 – 25 1

IEF Limits

Maximum volt, current per strip, and watt per strip are applied to the protocol. These settings will override the values in the individual steps. These overall limits can be used to avoid certain focusing conditions that cause damage or problems with the IPG strips.

The temperature for the focusing is also set. Since the isoelectric points of proteins are temperature dependent, IEF is typically at a single temperature, usually 20 °C. It is not recommended to use temperatures lower than 20 °C as it can cause problems with urea crystallization in the IPG strip.

Knob Computer Settable Settable Parameter Units Range Increments Increments

Volt (V) 0 – 12,000 250 1

Current/strip (μA) 0 – 999 25 1

Watts/strip (W) 0 – 2.0 0.1 0.1

Temperature (°C) 15 – 25 1 1

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Steps

To edit any step parameter, turn the knob to highlight the parameter. Press the knob, and turn the knob to set the desired value. Press the knob again to enter the value, and move to the next ﬁeld.

Each step has four ﬁelds that can be edited: the step type, a step value, a step time, and step units:

Step Type Constant volt, gradient volt, constant watt.

Step Value Sets the maximum value of volt or watt depending

on step type.

Step Time Time in hours or volt-hours.

Step Units Hours (Hrs) or volt-hours (Vhrs).

Step Type and Step Value

Note: It is not recommended to set

a series of discrete volt steps. The discontinuities between steps cause spikes in power levels that tend to increase the chances of burning strips.

Note: In some cases, the volt settings may never be reached.

Note: If a constant watt step is followed by a gradient volt step, the gradient volt step will start from 0, and increase linearly to the set voltage.

Each step can be programmed in one of three different modes, constant volt, gradient volt, or constant watt.

• In constant volt, the voltage remains constant thoughout the length of the step.

• In gradient volt, the voltage will start at the end voltage of the previous step and increase (or decrease) linearly over time to the voltage entered in the current step. If the ﬁrst step of the protocol is a gradient volt step, the voltage will increase from 0.

• Gradient volt steps gradually increase the power being applied to

the IPG strips, resulting in more even heating of the strip over time.

• Constant watt steps will smooth out the heat generation as much as possible over the course of the IEF separation. There will still be localized heating due to different ion concentrations along the length of the IPG strips.

• When using the constant watt step, the units for the step value

automatically change to watts.

• In constant watt steps, the IEF100 calculates the resistance, and

adjusts the voltage to maintain the constant watt setting.

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+ 45 hidden pages