Hoefer DQ300 User Manual

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user manual

Hoefer DQ300

Fluorometer

um DQ300-IM/Rev.C0/12-12

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Important Information ............................................ ii

Waste Electrical & Electronic Equipment (WEEE) ....vii

1. Introduction

1.1 Description .....................................................1

1.2 Inspection and Setup .......................................1

1.3 General Information and Precautions .................4

2. Quick View Diagrams .........................................5

3. Instrument Parameters

3.1 Firmware Flowchart .........................................6

3.2 Instrument Power Up .......................................8

3.3 Fluorescence Channels ....................................8

3.4 Calibration Standard Value ...............................8

3.5 Calibration ...................................................... 9

3.6 Sample Analysis ............................................ 10

3.7 Internal Data Logging (IDL) ............................ 12

3.8 Diagnostic Information ................................... 14

4. General Considerations for Analysis

4.1 Handling Samples .........................................15

4.2 Linear Range and Quenching ..........................16

4.3 Temperature Considerations ............................17

4.4 Positioning Samples ......................................17

4.5 Data Quality ..................................................17

5. Troubleshooting

5.1 Low or No Reading ........................................18

5.2 High or Over Reading ..................................... 19

5.3 Fluctuating Results ........................................ 19

5.4 Solid Standard ..............................................19

Appendix ............................................................20

Troubleshooting ................................................... 21

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Important Information – English

• If this equipment is used in a manner not specied by Hoefer, Inc. the protection provided by the equipment may be impaired.

• This instrument is designed for indoor laboratory useonly.

• Only accessories and parts approved or supplied by Hoefer, Inc. may be used for operating, maintaining, and servicing this product.

• Warning! Because this instrument can develop sucient voltage and current to produce a lethal shock, care must be exercised in its operation.

• This instrument is designed in accordance with the EN61010-1:2001 electrical safety standard. Nevertheless, it should be used only by properly trained operators. Read this entire manual before using the instrument and use only according to the instructions.

• The instrument must always be used with the earth lead of the power cord correctly grounded to earth at the mains outlet.

• Use only undamaged electrical wire and equipment specic for the voltages you will use. All equipment connected to high voltage should be in accordance with EN61010-1:2001.

• Keep the instrument as dry and clean as possible. Wipe regularly with a soft, damp cloth. Let the instrument dry completely before use.

• Do not operate the instrument in extreme humidity (above 80%). Avoid condensation by letting the unit equilibrate to ambient temperature when taking the instrument from a colder to a warmer environment.

• To permit sucient cooling, ensure that the vents of the instrument are not covered.

Duležité Informace – Czech

• Pokud by toto zařízení je použito způsobem, kter ý není podle Hoefer, ochrana poskytovaná na základě Inc. zařízení může být narušena.

• Tento nástroj je určen pro vnitřní použití v laboratořipouze.

• Pouze příslušenství a části schválen, nebo poskytnutých Hoefer, Inc. mohou být použity pro provoz, údržbu, a údržbě tohoto výrobku.

• Pozor! Protože tento nástroj může vyvinout dostatečný napětí a proud, který má vyrábět a smrtiacej šok, péče musí být vykonávána v jeho provoz.

• Tento nástroj je určen v souladu s EN61010-1:2001 elektrické bezpečnostní normy. Přesto, že by měly být

použity pouze řádně vyškolený operátorů. Číst celé toto ruční před použitím nástroje a použití pouze v souladu s pokyny.

• Přístroj musí být vždy používají se na výkonu zemi vést šňůra správně zemněny k zemi na síti v ýústce.

• Využití pouze nepoškozené elektrické dráty a vybavení pro napětí budete používat. Všechna zařízení spojené s vysokým napětím by měla být v souladu s EN610101:2001.

• Si ponechá nástroje jako suchý a čistý jako možné. Otřete pravidelně s a měkké, vlhk ým hadříkem. Nechť je nástroj nenastavený úplně před použitím.

• Nejsou provozována na nástroj v extrémní vlhkost (nad 80%). Předešlo kondenzaci o pronájmu jednotky na okolní teplotu nechá při přijímání nástroj z chladnější do teplého prostředí.

• Pro umožnění dostatečné chlazení, zajistit, aby otvory nástroje jsou nevztahuje.

Vigtig Information – Danish

• Hvis dette udstyr bruges i en måde ikke speciceret ved Hoefer, Inc. den beskyttelse, som er blevet forsynet af udstyret kan måske svæk kes.

• Dette instrument er designet for indendørs laboratoriumbrug bare.

• Bare tilbehør og del godkendede eller forsynede ved Hoefer, Inc. kan måske bruges for drive, funktionsfejl, og betjening dette produkt.

• Advare! Fordi dette instrument kan udvikle tilstrækkelig spænding og strøm at fremstille et dødbringende chok, skal pleje bruges i dets drift.

• Dette instrument er designet i overensstemmelse med EN61010-1:2001 elektrisk sikkerhedstandard. Alligevel, skulle det bruges bare af passende trænede operatører. Læs denne hel håndbog før bruging instrumentet og brug bare i henhold til instruktionerne.

• Instrumentet skal altid bruges med jordblyet af netledningen rigtigt jordede til jord på hovedledningsudløbet.

• Bruger bare uskadt elektrisk tråd og udstyr, som være specik for spændingerne du vil bruge. Alt udstyr forbundet til høj spænding skulle være i overensstemmelse med EN61010-1:2001.

• Beholder instrumentet så tør og ren som mulig. Tør regulært med et blødt, fugtigt stof. Lad instrumenttørken komplet før brug.

• Driver ikke instrumentet i yderst fugtighed (ovenfor 80%). Undgå kondensation ved lade enheden equilibrate til omgivende temperatur ved tageen instru-

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mentets fra et koldere til et varmere miljø.

• At tillade tilstrækkelig afkøling, forsikrer, at lufthullerne af instrumentet er ikke dækket.

Belangrijke Informatie – Dutch

• Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer is gespeciceerd, Nv. de bescherming die door de uitrusting is verzorgd kan worden geschaad.

• Dit instrument is voor binnenlaboratoriumgebruik enkel ontworpen.

• Enkel onderdelen en delen keurden goed of leverden door Hoefer, Nv. kan voor het bedienen worden gebruikt, handhavend en onderhouden van dit product.

• Waarschuwend! Omdat dit instrument voldoende spanning en stroom kan ontwik kelen om een dodelijke schok te produceren, moet zorg in zijn operatie worden geoefend.

• Dit instrument is in overeenstemming met de EN61010-1:2001 elektrische veiligheidsstandaard ontworpen. Niettemin zou het enkel door goed getrainde bedieningslieden moeten worden gebruikt. Lees dit volledige handboek voor het gebruik het instrument en gebruik enkel volgens de instructies.

• Het instrument moet altijd met de aardeleiding van het stroomsnoer correct grondde naar aarde aan het hoofdafzetgebied worden gebruikt.

• Gebruik enkel onbeschadigde elektrische draad en uitrustings speciek voor de spanningen u zult gebruiken. Alle uitrustingen sloten aan aan hoogspanning zou in overeenstemming met EN61010-1:2001 moetenzijn.

• Houd het instrument zo droge en schone zoals mogelijk Bij. Wis regelmatig met een zacht, temper doek. Verhuur het instrument droogt volledig voor hetgebruik.

• Bedien niet het instrument in extreme vochtigheid (bovenstaande 80%). Vermijd condensatie door het verhuren van de eenheid in evenwicht brengt naar omgevingstemperatuur wanneer nemen het instrument van een kouder naar een lievere omgeving.

• Om toe te staan voldoende afkoelen, verzeker dat de luchtopeningen van het instrument niet bedekt zijn.

Tärkeää Tietoa – Finnish

• Jos tätä varusteita käytetään tavassa ei määritetty Hoeferille, Inc. suojelu ehkäisty varusteille saattaa ollaavuton.

• Tämä väline suunnitellaan sisälaboratoriokäytölle vain.

• Vain lisävarusteet ja osat hyväksyivät tai toimitti Hoeferin oheen, Inc.:ää voi käyttää k äyttämiselle, valvoalle, ja servicing tämä tuote.

• Varoittaminen! Koska tämä väline voi kehittää riittävä jännitteen ja virran tuottaa kuolettavan järkytyksen, huolta täytyy harjoittaa toiminnossaan.

• Tämä väline suunnitellaan EN61010-1:2001 sähköturvallisuusstandardin mukaisesti. Silti pitäisi käyttää vain ohi oikeasti koulutetut k äyttäjät. Lue tämä kokonainen manuaalinen ennen välinettä ja k äyttö vain ohjeidenmukaan.

• Välinettä täytyy käyttää aina valtanuoran maalyijystä perusti oikein maadoittaa sähköverkkoaukossa.

• Käyttää vain undamaged sähkömetallilankaa ja varusteita, täsmällinen jännitteille käyttää. Kaik ki varusteet yhdistetty korkeaan jännitteeseen pitäisi olla EN610101:2001IN mukaisesti.

• Pitää välineen yhtä kuiva ja puhdas kuin mahdollinen. Pyyhi säännöllisesti pehmeällä, kostealla k ankaalla. Anna väline kuivua täysin ennen käyttöä.

• Ei käytä välinettä extreme-ilmankosteudessa (80%)n yläpuolella. Vältä tiivistymistä antamalla yksikön equilibrate ympäröivään lämpötilaan kun ottaminen väline kylmempi lämpimämpään ympäristöön.

• Sallia riittävän jäähdyttäminen, varmistaa että välineen ilmareiät peitetään.

Information Importante – French

• Si cet équipement est utilisé dans une manière pas spécié par Hoefer, Inc. la protection fourni par l’équipement pourrait être diminuée.

• Cet instrument est conçu pour l’usage de laboratoire intérieur seulement.

• Seulement les accessoires et les parties ont approuvé ou ont fourni par Hoefer, Inc. pourrait être utilisé pour fonctionner, maintenir, et entretenir ce produit.

• Avertissant! Parce que cet instrument peut développer la tension et le courant susants pour produire un choc mortel, le soin doit être exercé dans son opération.

• Cet instrument est conformément conçu à l’EN610101:2001 norme de sécurité électrique. Néanmoins, il devrait être seulement utilisé par les opérateurs convenablement entraînés. Lire ce manuel entier avant utiliser l’instrument et l’usage seulement selon les instructions.

• L’instrument toujours doit être utilisé avec l’avance de terre du cordon d’alimentation correctement a fondé à

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la terre à la sortie principale.

• Utiliser le l et l’équipement électriques seulement intacts spéciques pour les tensions que vous utiliserez. Tout équipement connecté à haute tension devrait être conformément à EN61010-1:2001.

• Garder l’instrument aussi sec et propre comme possible. Essuyer régulièrement avec un doux, étouer du tissu. Laisser l’instrument sèche complètement avant l’usage.

• Ne pas fonctionner l’instrument dans l’extrême humidité (au-dessus de 80%). Eviter la condensation en laissant l’equilibrate d’unité à la température ambiante en prenant l’instrument d’un plus froid à un environnement plus chaud.

• Permettre le refroidissement susant, garantir que les conduits de l’instrument ne sont pas couverts.

Wichtige Informationen – German

• Wenn diese Ausrüstung gewissermaßen nicht angegeben durch Hoefer, Inc verwendet wird, kann der durch die Ausrüstung zur Verfügung gestellte Schutz verschlechtert werden.

• Dieses Instrument wird für den I nnenlaborgebrauch nur dafür entworfen.

• Nur Zusätze und Teile genehmigten oder lieferten durch Hoefer, Inc kann für das Funktionieren, das Aufrechterhalten, und die Wartung dieses Produktes verwendet werden.

• Die Warnung! Weil dieses Instrument genügend Stromspannung und Strom entwickeln kann, um einen tödlichen Stoß zu erzeugen, muss Sorge in seiner Operation ausgeübt werden.

• Dieses Instrument wird in Übereinstimmung mit dem EN61010-1:2001 elektrischen Sicherheitsstandard dafür entworfen. Dennoch sollte es nur von richtig erzogenen Maschinenbedienern verwendet werden. Lesen Sie dieses komplette Handbuch vor dem Verwenden des Instrumentes und verwenden Sie nur gemäß den Instruktionen.

• Das Instrument muss immer mit der Erdleitung der Macht-Schnur richtig niedergelegt zur Erde am Hauptausgang verwendet werden.

• Nur unbeschädigte elektrische Leitung und Ausrüstung spezisch für die Stromspannungen verwenden, die Sie verwenden werden. Die ganze mit der Hochspannung verbundene Ausrüstung sollte in Übereinstimmung mit EN61010-1:2001 sein.

• Das Instrument ebenso trocken halten und reinigen wie möglich. Wischen Sie regelmäßig mit einem weichen, befeuchten Sie Sto. Lassen Sie das Instru-

ment trocken völlig vor dem Gebrauch.

• Das Instrument in der äußersten Feuchtigkeit (über 80 %) nicht bedienen. Vermeiden Sie Kondensation, die Einheit equilibrate zur Umgebungstemperatur laßend, wenn Sie das Instrument von einem kälteren bis eine wärmere Umgebung nehmen.

• Um das genügend Abkühlen zu erlauben, stellen Sie sicher, dass die Önungen des Instrumentes nicht bedeckt werden.

Informazioni Importanti – Italiano

• Se quest’apparecchiatura è usata in un modo specicato da Hoefer, Inc. la protezione fornito dall’apparecchiatura potrebbe essere indebolita.

• Questo strumento è disegnato per l’uso di laboratorio interno solo.

• Solo gli accessori e le parti hanno approvato o hanno fornito da Hoefer, Inc. potrebbe essere usato per operare, per mantenere, e per revisionare questo prodotto.

• Avvertendo! Perché questo strumento può sviluppare il voltaggio suciente e la corrente di produrre una scossa letale, la cura deve essere esercitata nella sua operazione. Questo strumento è disegnato conformemente all’EN61010-1:2001 la norma di sicurezza elettrica. Tuttavia, dovrebbe essere usato degli operatori solo correttamente addestrati. Leggere questo manuale intero prima di usare lo strumento e l’uso solo secondo le istruzioni.

• Lo strumento deve essere sempre usato col piombo di terra della spina di alimentazione correttamente hanno messo a terra alla terra alla presa di corrente principale.

• Usa il lo metallico e l’apparecchiatura solo intatti elettrici specici per i voltaggi che lei userà. Tutta l’apparecchiatura collegata all’alto voltaggio dovrebbe essere conformemente a EN61010-1:2001.

• Tiene lo strumento come secco e pulito come possibile. Pulire regolarmente con un morbido, per spegnere il panno. Lasciare lo strumento asciuga completamente prima dell’uso.

• Non opera lo strumento nell’umidità estrema (al di sopra di 80%). Evitare la condensazione lasciando l’unità equilibra alla temperatura ambiente quando portare lo strumento da un più freddo a un ambiente più caldo.

• Di permettere rareddare suciente, assicura che gli sbocchi dello strumento non sono coperti.

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Viktig Informasjon – Norwegian

• Hvis dette utstyret blir brukt i en måte ikke spesisert ved Hoefer, Inc. beskyttelsen som ha blitt git av utstyret kan bli svekket.

• Dette instrumentet er utformet for innendørs laboratoriumbruk bare.

• Bare tilbehør og deler godkjente eller forsynte ved Hoefer, Inc. kan bli brukt for drive, vedlikeholde, og betjene dette produktet.

• Varsler ! Fordi dette instrumentet kan utvikle tilstrekkelig spenning og strøm til å produsere et dødelig sjokk, må bli øvd bek ymring i dets drift.

• Dette instrumentet er utformet i samsvar med EN61010-1:2001 elektrisk sikkerhetsstandard. Likevel burde bli brukt det bare av sk ikkelig utdannede operatører. Les denne hele håndboken før bruking instrumentet og bruken bare gi til instruksjonene.

• Instrumentet må alltid bli brukt med jorden blyet av kraftkabelen som riktig ha blitt jordet til jord på hovedledningen utløp.

• Bruker bare uskadd elektrisk ledningsfremføring og utstyr som er spesikk for spenningene du vil bruke. All utstyr koplet til høyspenning burde være i samsvar med EN61010-1:2001.

• Beholder instrumentet som tørker og rengjør som mulig. Visk regulært med et mykt, fuktig sto. La instrumentet tørker komplett før bruk.

• Driver instrumentet i ekstrem fuktighet ikke (ovenfor 80%). Unngå kondensasjon ved å la enheten equilibrate til omgivelsestemperatur ved taen instrumentets fra et kaldere til et varmere miljø.

• Til å tillate tilstrekkelig kjølig, sikrer at ventilasjonsåpningene av instrumentet er ikke dekket.

Wazne Informacje – Polish

• Jeżeli ten sprzęt jest wykorz ystywany w sposób nie określone przez Hoefer, Inc. do ochrony przewidzianej przez urządzenie może zostać obniżony.

• Instrument ten jest przeznaczony do uż ytku w laboratoriach kryty tylko.

• Tylko akcesoriów i części zatwierdzone lub dostarczone przez Hoefer, Inc. mogą być wykorzystane do eksploatacji, utrzymania i obsługi tego produktu.

• Uwaga! Ponieważ ten akt prawny może być rozwinięcie odpowiednich napięcie i bieżących do wyprodukowania śmiertelnego szoku, opiekę musi być wykonywane w działaniu.

• Ten instrument został zaprojektowany zgodnie z tym EN61010-1: 2001 Bezpieczeństwo elektr yczne standard. Niemniej jednak, należy stosować jedynie przez odpowiednio przeszkoleni operatorów. Znajdą państwo to cały podręcznika przed zastosowaniem instrumentu i stosować jedynie zgodnie z instrukcjami.

• Instrument musi zawsze być wykorz ystane z ziemi doprowadzić do zasilania detonującego właściwie uzasadnione na ziemię w sieci wodociągowej rynkuzbytu.

• Wykorzystanie tylko nieuszkodzona elektr ycznych drutów i urządzenia specjalne do napięć zapłacą wykorzystania. Wszystkie urządzenia podłączone do wysokiego napięcia powinny być zgodne z EN61010-1:

2001.

• Kontrolować instrumentu jako suche i czyste jak to możliwe. Wytrzeć regularnie przy pomocy miękk iego wilgotnej szmatki. Niech się instrumentem całkowicie wysuszyć przed użyciem.

• Nie prowadzą do instrumentu w skrajnych wilgotności (powyżej 80%). Zapobiec kondensacji najmu przez jednostkę równoważyć do temperatury pokojowej przy podejmowaniu instrumentu z chłodniejsze w cieplejszych środowiska.

• Aby umożliwić wystarczające chłodzenia, zapewniają, że rozcięcia of the instrument nie objęte ubezpieczeniem.

Informações Importantes – Portuguese

• Se este equipamento é usado numa maneira não especicada por Hoefer, Inc. que a protecção fornecida pelo equipamento pode ser comprometida.

• Este instrumento é projectado para uso de interior de laboratório só. Só acessórios e partes aprovaram ou forneceu por Hoefer, Inc. pode ser usada para operar, manter, e servicing este produto.

• Advertindo! Porque este instrumento pode desenvolver voltagem suciente e corrente produzir um choque letal, cuidado deve ser exercitado em suaoperação.

• Este instrumento é projectado de acordo com o EN61010-1:2001 condição de segurança eléctrica. Não obstante, deve ser usado só por operadores adequadamente treinados. Leia este manual inteiro antes de usar o instrumento e use só de acordo com as instrucções.

• O instrumento sempre deve ser usado com o chumbo de terra do cordão de poder corretamente baseou a

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terra nos canos saída principais.

• Usa o eléctrico só intacto e equipamento especíco para as voltagens que você usará. Todo equipamento conectado a voltagem alta deve ser de acordo com EN61010-1:2001.

• Mantem o instrumento tão seco e limpo como possível. Limpe regularmente com um pano húmido macio. Deixe o instrumento secar completamente antes de uso.

• Não opera o instrumento em humidade extrema (acima de 80%). Evite condensação deixando o equilibrate de unidade a temperatura ambiental quando tomar o instrumento de um mais frio a um ambiente mais quente.

• Permitir esfriar suciente, assegura que as aberturas do instrumento não são cobertas.

Información Importante – Spanish

• Si este equipo es utilizado en una manera no especicado por Hoefer, S.a. la protección proporcionado por el equipo puede ser dañada.

• Este instrumento es diseñado para el uso interior del laboratorio sólo. Sólo accesorios y partes aprobaron o suministraron por Hoefer, S.a. puede ser utilizado para operar, para mantener, y para atender a este producto.

• Advirtiendo! Porque este instrumento puede desarrollar voltaje y corriente sucientes para producir un golpe mortal, el cuidado debe ser ejercitado en suoperación.

• Este instrumento es diseñado de acuerdo con el EN61010-1:2001 estándar eléctrico de seguridad. No obstante, debe ser utilizado sólo por operarios adecuadamente capacitados. Lea este manual entero antes de utilizar el instrumento y el uso sólo según las instrucciones.

• El instrumento siempre debe ser utilizado con el plomo de la tierra del cable de alimentación molió correctamente a la tierra en la salida de red.

• Utiliza alambre y equipo eléctricos sólo ilesos especícos para los voltajes que usted utilizará. Todo equipo conectado al voltaje alto debe ser de acuerdo con EN61010-1:2001.

• Mantiene el instrumento tan seco y limpio como posible. Enjugue regularmente con un suave, el trapo húmedo. Permita que el instrumento seque completamente antes de uso.

• No opera el instrumento en la humedad extrema

(encima de 80%). Evite condensación permitiendo la unidad equilibra a la temperatura ambiente al tomar el instrumento de un más frío a un ambiente más tibio.

• Permitir refrigeración suciente, asegure que las aberturas del

Viktig Information – Swedish

• om denna utrustning används i ett sätt som inte har speciceras av Hoefer, Inc. skyddet tillhandahöll vid utrustningen kan skadas.

• Detta instrument formges för inomhuslaboratorium användning bara.

• Bara medhjälpare och delar godkände eller levererade vid Hoefer, Inc. kan användas för fungera, underhålla, och servicing denna produkt.

• varna! Därför att detta instrument kan utveckla tillräcklig spänning och ström att producera en dödlig stöt, måste övas omsorg i dess funktion.

• Detta instrument formges i överensstämmelse med EN61010-1:2001 elektriska säkerheten standarden. Icke desto mindre, bör det användas bara av riktigt utbildade operatörer. Läs denna hela handbok före använda instrumentet och använd bara enligt undervisningarna.

• Instrumentet måste alltid användas med jorden blyet av kraften repet riktigt grounded till jorden på det huvudutloppet.

• Använder bara undamaged elektrisk tråd och utrustning specik för spänningarna du sk a använda. All utrustning kopplats som till hög spänning skulle vara i överensstämmelse med EN61010-1:2001.

• Håller instrumentet då torkar och rengör som möjlig. Torka regelbundet med en mjuk, fuktig trasa. Låt instrumentet torka fullständigt före användningen.

• Fungerar inte instrumentet i extrem fuktighet (över 80%). Undvik kondensering vid låta enheten equilibrate till omgivande temperatur när ta instrumentet från en kallare till en varmare miljö.

• Att tillåta tillräcklig kyla, ser till att hålen av instrumentet inte täcks.

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Waste Electrical and Electronic Equipment (WEEE)

English

French

German

Italian

Spanish

This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment.

Ce symbole indique que les déchets relatifs à l’équipement électrique et électronique ne doivent pas être jetés comme les ordures ménagères non-triées et doivent être collectés séparément. Contactez un représentant agréé du fabricant pour obtenir des informations sur la mise au rebut de votre équipement.

Dieses Symbol kennzeichnet elektrische und elektronische Geräte, die nicht mit dem gewöhnlichen, unsortierten Hausmüll entsorgt werden dürfen, sondern separat behandelt werden müssen. Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf, um Informationen hinsichtlich der Entsorgung Ihres Gerätes zu erhalten.

Questo simbolo indica che i riﬁuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come riﬁuti municipali indifferenziati e devono invece essere raccolti separatamente. Per informazioni relative alle modalità di smantellamento delle apparecchiature fuori uso, contattare un rappresentante autorizzato del fabbricante.

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Swedish

Denna symbol anger att elektriska och elektroniska utrustningar inte får avyttras som osorterat hushållsavfall och måste samlas in separat. Var god kontakta en auktoriserad tillverkarrepresentant för information angående avyttring av utrustningen.

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1. Introduction

1.1 Description

The DQ300 Fluorometer is a dual-channel ﬂuorometer designed for quick, easy, and accurate ﬂuorescence measurements. When properly calibrated with a standard of known concentration, the DQ300 displays the actual concentration of the compound. The new Minicell adaptor design enables user to measure compounds at a low concentration with a low assay volume (e.g, dsDNA at 1 ng/mL using PicoGreen®, and RNA at 1 ng/mL using RiboGreen®).

1.2 Inspection and Setup

1.2.1 Inspection

Upon receiving your instrument, please inspect everything carefully and make sure all accessories are present (refer to the packing list shipped with the instrument).

All shipments include:

1. DQ300 Fluorometer

2. DQ300 User’s Manual

3. DQ300 Quick Reference Guide

4. Power supply

5. RS-232 cable

6. Spreadsheet Interface software (1 CD) and Instructions

For spare parts information, contact support@hoeferinc.com or 1-800-227-4750.

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Unpacking

This declaration of conformity is only valid for the instrument when it is:

• used in laboratory locations.

• used as delivered from Hoefer, Inc. except for alterations described in the user manual.

• connected to other CE labeled instruments or products recommended or approved by Hoefer, Inc.

Unwrap all packages carefully and compare contents with the packing list, making sure all items arrived. If any part is missing, contact your local sales ofﬁce. Inspect all components for damage that may have occurred while the unit was in transit. If any part appears damaged, contact the carrier immediately. Be sure to keep all packing material for damage claims or for repacking should it become necessary to return the unit.

Instrument Specifications

Size 2.7" × 5.5" × 7.25"

(6.9 cm × 14 cm × 18.42 cm)

Weight 1.8 lbs (0.67 kg)

Dynamic range 3 orders of magnitude

ADC Resolution 12 bits

LCD Display 2 × 16 characters

Operating Temp. 59–98 F; 15–40 °C

Detectors Photodiodes: measurement

capability from 300–1000 nm

Calibration Type Single-point and blank

Alarms High blank

Cuvette Type 10 × 10 mm plastic, 10 × 10 mm Minicell Adaptor

Warm Up Time 5 seconds

Optical Specifications

Light Source UV LED Blue LED

Excitation Optics 365–395 nm 465–485 nm

Emission Optics 440–470 nm 515–575 nm

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1.2.2 Setup

To get started (Refer to the Quick View Diagram, page 5):

Position the instrument on a ﬂat surface.

Assemble the power supply: choose the proper AC clip for your outlet, and slide it on to the AC adaptor.

Plug in the unit. Be sure to use only the power supply provided with your instrument by Hoefer, Inc.

Powering the instrument with an unapproved power supply may damage the unit.

Turn on the instrument by pressing the <ON/OFF> button. The warm up screen should appear on the display.

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1.3 General Information and Precautions

The sample compartment cannot accept glass or quartz cuvettes but can accept methacrylate or polystyrene cuvettes.

A minimum volume of 2 mL is required when using the 10 × 10 mm cuvette.

A minimum volume of 50 µL in a Minicell is required.

Avoid having any air bubbles in your sample. They can signiﬁcantly affect the ﬂuorescence reading.

When the Minicell is used, make sure to place the Minicell adaptor in the correct position. If BLUE (B) channel is selected, insert the Minicell adaptor in the sample compartment with the tab away from you and the “BLUE” label facing you.

If UV (UV) channel is selected, insert the Minicell adaptor in the sample compartment with the tab nearest to you and the “UV” label facing you (Refer to Quick View Diagram, page 5).

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2. Quick View Diagrams

Sample Compartment

LCD Screen

ON/OFF Button

Positioning the Minicell Adaptor

10 × 10 mm Cuvette Holders

Minicell Cuvette Holders

BLUE Position

UV Position

Power Supply Input

Serial Port

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3. Instrument Parameters

3.1 Firmware Flowchart

DQ 300

5 second warm up

Indicates which channel

is active

Press <UV/B> to

switch between

XXX.X UV

and

XXX.X BLUE

Press <STD VAL>

Cal Val: XXX.X

<UP/DOWN> UV

Home Screen

Press <CAL>

Calib. UV

<ENTER> to start

Insert Blank then

press <ENTER>

Reading Blank XX.X

Insert Cal soln then

press <ENTER>

Reading Cal Soln

XX.X

Calib. Completed

Press <ENTER>

If <ENTER> is not

pressed within

seconds, this

will appear

XXX.X

Read Mode:

Discrete

Reading UV

WAIT XXX.X

Logging Points Left: XXX

%FS-BLK: XX.X

%FS-STD: XX.XX

Fluorescence reading Indicates activity of IDL

Press <UP> or <DOWN>

to adjust contrast

Press <SET>

Press <ENTER>

Press <DIAG> then

<ENTER> to

le between

Press <ENTER>

Press <UP> or <DOWN>

Abort Cal?

<UP>Yes

<DOWN> No

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<DOWN>

Press <SET>

Read Mode:

Continuous

Press <ENTER>

Continuous Meas Interval: 5 sec

Press <SEND>

Datalogger:

Press <ENTER>

Press <ENTER> 2X

Download data: 5X<SEND> to start

Press <UP> or <DOWN>

to select interval

Press <ENTER>

Continuous Meas

Count: (10-100): 10

Press <UP> or <DOWN>

to select sample

measured

Press <ENTER>

XXX.X UV

Press <READ>

Cont: Count = 10

<ENTER> int = 60 sec

Press <ENTER>

Insert sample Press <ENTER>

READING # 1 /10

Waiting #1 /10 XXX.X

Download data: All downloaded

If there is no data

logged, this screen

Data logger has no valid data!

Press <ENTER> 1X

Status: Stop <UP > to toggle

<UP>

Status: Logging <UP> to toggle

will appear

Press <ENTER> 3X

Erase Data 5X<SEND> to start

Erase Data All data erased

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3.2 Instrument Power Up

To turn on the DQ300, press the <ON/OFF> button. The instrument takes 5 seconds to warm up. After warming up, the DQ300 is ready for operation.

To adjust the display contrast press the <UP> or <DOWN> buttons.

3.3 Fluorescence Channels

Choose the appropriate channel for your analysis. To do this, press the <UV/B> button to toggle between the 2 channels. The activated channel name will be displayed in the lower left corner of the Home Screen.

UV = Ultraviolet excitation Blue = Blue light excitation

See page 2 for Optical (Wavelength) Speciﬁcations.

3.4 Calibration Standard Value

Before performing a calibration, assign a convenient numeric value between 1 and 999 for your standard solution. (e.g. enter 100 if your calibration standard is 100 ng/mL).

Press the <STD VAL> button.

Use the <UP> or <DOWN> button to adjust the standard value. Holding the button down will activate faster scrolling.

When ﬁnished, Press <ESC> or <ENTER> to accept the value and return to the Home Screen.

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Note: If using the Minicell adaptor, be sure to insert it in the correct position (see 1.3.5 on page 4).

3.5 Calibration

Press the <CAL> button.

Press <ENTER> to start the calibration.

Insert your blank and press <ENTER>. The DQ300 will average the ﬂuorescence of the blank for 5 seconds.

Insert the calibration standard and press <ENTER>. The DQ300 will average the ﬂuorescence of the standard for 5 seconds.

Press <ENTER> when the calibration is complete to accept the calibration. If <ENTER> is not pressed within 10 seconds, you will be asked if you want to abort the calibration. Press the <UP> or <DOWN> button to abort or accept the calibration, respectively.

If at any time during steps 1–4 you want to stop the calibration, press <ESC>. The display will return to the Home Screen and default back to the previous calibration.

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3.6 Sample Analysis

You can read samples in two reading modes: Discrete or Continuous.

3.6.1 Discrete Mode

In Discrete Mode, the instrument measures and averages the ﬂuorescence signal over a 5-second interval.

Press the <SET> button.

Use the <UP> or <DOWN> button to select the “Discrete” mode.

Press <ENTER>.

Insert your sample.

Press <READ> button. The result will be displayed at the top and center of the Home Screen.

The top left corner will display “WAIT” for 5 seconds. Once “WAIT” disappears, another sample reading can be performed.

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Page 19

3.6.2 Continuous Mode

In Continuous Mode, the instrument measures and averages the ﬂuorescence signal over a 5-second interval for each sample measurement. The instrument measures the sample repeatedly for a user deﬁned sample number.

Press the <SET> button.

Use the <UP> or <DOWN> button to select the “Continuous” mode.

Press <ENTER>.

Use the <UP> or <DOWN> button to select a measurement interval.

Press <ENTER>.

Use the <UP> or <DOWN> button to select the number of samples you want to measure.

Press <ENTER>.

Press <READ> button.

The screen will display the setting selected.

Press <ENTER>.

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Page 20

Insert your sample and Press <ENTER> again.

The result will be displayed at the top center of the Home Screen.

To abort the continuous sample measuring, press <ESC> and <ENTER>.

3.7 Internal Data Logging (IDL)

The DQ300 can log up to 1000 data points to the internal memory. The DATA screen controls logging, downloading and erasing of data.

3.7.1 Activating Data Logging

Press the <SEND> button.

Press <ENTER>.

Press the <UP> or <DOWN> button to toggle between logging and stop.

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Press <ESC> when ﬁnished to return to the Home screen.

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Page 21

3.7.2 Downloading Data

Connect the DQ300 to the serial port of a PC using the provided or standard interface cable.

Launch the Spreadsheet Interface software. See Appendix for computer requirements and installation instructions.

Press the <SEND> button.

Press <ENTER> 2 times.

Press <SEND> 5 times to start the data download.

Press <ESC> when ﬁnished to return to the Home Screen.

3.7.3 Erasing Data

Press the <SEND> button.

Press <ENTER> 3 times.

Press <SEND> 5 times to erase all logged data.

Press <ESC> when ﬁnished to return to the Home Screen.

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Page 22

3.8 Diagnostic Information

Press <DIAG> to access the diagnostic screens.

The ﬁrst screen shows the number of data points available for internal data logging.

Press <ENTER> to toggle to the %FS (Full Scale) values from the calibration blank and standard.

Press <ESC> when ﬁnished to return to the Home Screen.

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Page 23

4. General Considerations for Analysis

4.1 Handling Samples

Be cautious not to spill samples into the sample chamber. Promptly wipe any spills.

The DQ300 is very sensitive and even small amounts of material from a previous sample may result in errors. Use a clean cuvette or disposable cuvette for each reading.

Fill the cuvette to the minimum required volume or a signiﬁcant error will result. A minimum volume of 2 mL in a 10 × 10 mm cuvette, or 50 µL in a Minicell, is required for best results.

The cuvette MUST BE CLEAN AND DRY on the outside when taking readings. Moisture and condensation on the outside can result in error.

Minute bubbles in samples will cause errors in readings. Be sure not to introduce bubbles into samples. Particular care must be taken with the Minicell. Slight tapping on the outside cuvette wall will often help dissipate bubbles.

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Page 24

4.2 Linear Range and Quenching

0 2 4 6 8 10 12

Fluorophore conc.

Relative Fluorescence Unit

The linear range is the concentration range in which the readout of the DQ300 is directly proportional to the concentration of the ﬂuorophore. The linear range begins with the smallest detectable concentration and extends to an upper limit (concentration) that is dependent upon the properties of the ﬂuorescent material, the ﬁlters used, and the path length. See the diagram below.

A nonlinear relationship is seen at higher concentrations where the ﬂuorescence signal increases more slowly than the change in concentration. At even higher concentrations, readings will begin to decrease even though the sample concentrations are increasing. This effect is known as “signal quenching.”

Linearity may be checked by diluting a sample 1:1 or some other convenient ratio (be sure to use an appropriate blank for the dilutions). If the sample is within the linear range, the reading will decrease in direct proportion to the dilution. If the reading does not decrease in direct proportion to the dilution or if the reading increases, the sample is beyond the linear range of your ﬂuorophore.

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Page 25

4.3 Temperature Considerations

Fluorescence is temperature dependent. As the temperature of the sample increases, the ﬂuorescence decreases. For greatest accuracy, read the blank, standard, and samples at the same temperature.

4.4 Positioning Samples

For low concentration samples, cuvettes often will give slightly different measurements depending upon their orientation in the sample compartment. This is due to defects in the shape of the cuvette that are not visible to the human eye. We recommend that the cuvette be marked at the top and positioned in the sample compartment the same way each time to minimize error.

4.5 Data Quality

The DQ300 is only as accurate as the standards that are used to calibrate it. It is important to follow good laboratory practices when preparing all solutions and reagents including standards, samples, and blank.

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Page 26

5. Troubleshooting

Following are troubleshooting tips for problems the user may encounter.

5.1 Low or No Reading

1. Are all the reagents and standards made to speciﬁcation? Are any of the reagents expired? Is the dilution done correctly?

2. Has the sample been exposed to light for an extended period?

Some ﬂuorescent dyes are susceptible to photodeg-

radation. It is recommended that the dye solution be protected from the light.

3. Is the correct optical channel selected for the application? Has the instrument been calibrated prior to reading?

4. Is the sample in a detectable, linear range for the instrument?

Sample concentration might be too low or too high

to be detected (see 4.2 on page 16).

5. Is the right type of cuvette being used?

Methacrylate cuvettes should be used for UV

applications.

6. Does the sample volume meet the minimum requirement?

The minimum volume is 2 mL for a 10 × 10 mm

cuvette, or 50 µL for a Minicell.

7. Is the a Minicell adaptor inserted in the correct orientation?

The Minicell adaptor needs to be placed in the

correct position.

If the BLUE channel is selected, insert the Minicell

adaptor in the sample compartment with the tab away from you and the “BLUE” label facing you.

If the UV channel is selected, insert the Minicell

adaptor in the sample compartment with the tab nearest to you and the “UV” label facing you.

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Page 27

8. What is the %FS (% of full scale) of blank and calibration standard?

Press <DIAG> and <ENTER> to display the %FS

values (see 3.8 on page 14). If you calibrated the instrument with the highest concentration standard and the %FS of the standard is too close to the %FS of the blank, you might be reading the samples out of the instrument’s detection limit.

5.2 High or Over Reading

Dilute samples and standards.

5.3 Fluctuating Results

1. Check for spills in the sample compartment.

2. Check the volume of the sample.

The minimum volume is 2 mL for a 10 × 10 mm

cuvette, or 50 µL for a Minicell.

3. Check if the reagents and cuvette material are compatible.

4. Check temperature (see 4.3 on page 17).

5. Conﬁrm that the %FS STD is greater than %FS BLK by factor of 2 or 3.

5.4 Solid Standard

The optional Solid Standard can be used to determine if the instrument is functioning properly.

The Solid Standard is designed to effectively verify the stability of the instrument or if any instrument settings have been changed. After calibration, insert the Solid Standard into the sample compartment of the DQ300 Fluorometer (Note: always insert it with the tab away from you), and record the reading. You can check the calibration or the stability of the instrument by reading the Solid Standard again in the future.

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Appendix: Internal Datalogging

Shipping Checklist

1. RS-232 Cable

2. Spreadsheet Interface software (1 CD) and instructions.

Both of these items are necessary for retrieving the stored data in the DQ300.

Hardware Requirements

1. PC with Windows® 95 or later

2. MS Excel 5.0 or later

3. At least 1 available serial port

Software Installation

1. Exit all Windows programs.

2. Insert the CD and run the setup program.

3. The setup wizard will install the necessary ﬁles.

4. When the setup is complete, an icon named “Spreadsheet Interface Software” will appear in the “Programs” menu.

5. Restart your computer.

Connecting

1. Using the provided or standard RS-232 cable, connect the 9 pin connector of the cable into the available serial port of your computer.

2. Plug the opposite end of the cable into the base of the DQ300.

3. Open the Spreadsheet Interface software.

4. Click on the box to the right of the COM port icon to select the appropriate COM port. This is usually COM port 2.

5. Click on “Start”. The program will ﬁnd and open MS Excel and should be ready for data transfer. The boxes to the left of the COM port and MS Excel should both be green.

6. Follow the directions from Section 3.7 for collecting and downloading data from the DQ300. Data will automatically appear in the MS Excel spreadsheet. Be sure to save this data before closing the software.

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Troubleshooting

Difﬁculties can arise when parameters are set incorrectly or cable connections are not secure. Here are some common problems.

1. The box to the left of the COM port is red. This means that the COM port is not available.

Causes:

a. Another instrument or program (such as palm pilot/

hot sync) could be occupying the port, making it unavailable. Make sure to close all programs of this type before downloading data.

b. The selected port is incorrect. Follow step 4

(Connecting) to choose another COM port.

2. All lights are green, but no data has been transferred, even though the instrument shows “All data downloaded”.

a. The connection between the DQ300 and the

computer is bad. Check and tighten the cable connections. Make sure both ends of the cable are plugged in tightly.

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Hoefer, Inc.

84 October Hill Road Holliston, MA 01746

Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: support@hoeferinc.com Web: www.hoeferinc.com

Hoefer is a registered trademark of Hoefer, Inc.

PicoGreen and RiboGreen are registered trademarks of Molecular Probes, Inc.

Printed in the USA.