Hitachi Koki CS150NX Instruction Manual

Important

2014.11

HITACHI

TABLETOP

MICRO ULTRACENTRIFUGE

CS150NX

INSTRUCTION MANUAL

－

Before using this CENT RIFUGE carefully read
through this INSTRUCTION MANUAL t o ensure
efficient, safe oper at i on.
Keep this INSTRUCTIO N M ANUAL available as an
important referenc e when using the CENTRIFUGE.

－

MFG.No. CAT.No. Manual code

● The appearance or speci ficat ion of the products covered in this manual is
subject to partial change f or i mprovement.

S99806110

Copyright © 2013 Hitachi Koki Co., Ltd.

The names of actual companies and products mentioned herein may be the trademarks of their respective owners.

All rights reserved. No part of this document may be reproduced or transmitted in any form or any means without
permission from Hitachi Koki Co., Ltd.

General description

The CS150NX is designed to separate liquid-suspended materials having different densities and
particle size.
The CS150NX is a product that pursues user-friendliness and reliability based on our many years
of experience in developing centrifuges. This centrifuge offers many features that we are confident
of satisfying your requirements. These features include the following.

1. The maximum speed is 150,000 rpm (1,050,000 x g).

2. Small and compact tabletop type

3. The touchscreen with easy-to-see color liquid crystal screen is incorporated.

4. The displayed language can be switched over between Japanese and English.

5. Various alarm indications can notify users of the causes and necessary actions of the troubles.
It can realize troubleshooting easier and quicker.

6. The rotor can be set by simply mounting it on the drive shaft in the rotor chamber (quick setting
type.)

7. Samples can be easily balanced visually (see Section 2-1-2).

8. This product spins very quietly, and is thus well suited for personal use.

9. A CFC-free thermomodule cooling system is employed featuring a powerful cooling capacity.

(Samples can be kept at 0℃ at maximum speed when the ambient temperature is 30℃. )

10. The real-time control (RTC) feature enables setting a start time or a finish time, thus letting you

run your machine at a desired date and time.

11. Centrifugal force (RCFmax and RCFavg) can be displayed and set (Note 1).

12. Twenty varieties of nine stepped modes can be programmed for a wide range of applications

such as step running.

13. In addition to a door lock and an imbalance detector, two independent microprocessors are

incorporated for overspeed detection (a dual CPU overspeed prevention mechanism) for
even greater safety.

4

Note 1: RCF: Relative Centrifugal Force

SAFETY NOTICES

r

Safety reminders

Carefully read and fully understand the following safety instructions.

●

Operate your instrument according to the instruction manual.

●

Be sure to observe the all safety precautions in the instruction manual and safety
instructions on your instrument. If neglected, personal injury and/or instrument damage
can be caused.

●

If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.

●

The safety reminders are indicated as shown below. The signal words "DANGER",
"WARNING" and "CAUTION" are indicated together with the hazard alert symbols in this
manual.

DANGER

：This note indicates an imminently hazardous situation, which if not

strictly observed, could result in personal severe injury or possible
death.

WARNING

：This note indicates a potentially hazardous situation, which if not

strictly observed, could result in personal severe injury o
possible death.

CAUTION

：This note indicates a potentially hazardous situation, which if not

strictly observed, could result in personal injury or severe damage
to the instrument.

This hazard alert symbol indicated together with a signal word is a
reminder to emphasize important safety instructions.

"NOTE" indicates a note which has no direct bearing on personal safety.

Do not perform any operation not specified in the instruction manual. If any problem is

found on your instrument, contact a Hitachi Koki authorized sales/service representative.
Although the safety precautions in the instruction manual and safety instructions on your

instrument have been fully considered, an unexpected situation may arise. Observe the
instructions in the instruction manual and always be careful yourself when operating this
instrument.

i

SAFETY NOTICES

○Mechanical Safety

WARNING：

●

Do not open the door while the rotor is spinning.

●

Do not attempt to slow or stop the spinning rotor by hand.

●

Do not incline or move the instrument while the rotor is spinning. Do not place
any object on the instrument or lean on the instrument.

●

Do not attempt to unlock the door forcefully while the rotor is spinning.

●

For operator safety, maintain a 30-cm "clearance envelope" around the
instrument while the rotor is spinning. Do not store dangerous substances
capable of developing flammable or explosive vapors on nor near the
centrifuge.

●

Repairs, disassembly, and other modifications to the centrifuge are strictly
prohibited unless performed by a Hitachi Koki authorized sales/service
representative.

●

Do not use the other’s manufacturer’s rotor without Hitachi Koki’s permission.

●

Always use a quick-setting rotor for this ultracentrifuge. The screw-type rotors
are inapplicable.

●

Check the chemical resistance chart attached to the rotor, and do not use any
sample inapplicable to the rotor (including the buckets). Using such a
sample could corrode the rotor (including the buckets).

●

Do not exceed the maximum rated speed of the rotor or buckets in use.

●

Do not use corroded, scratched or cracked rotor and buckets. Check that the
rotor and buckets are free of such abnormalities before operation.

●

When using a swing rotor, check that the buckets are properly engaged with
the rotor pins before operation. Wrong setting can cause severe damage to
the instrument. Make sure that all the rotor buckets are of the same type.

●

If abnormal sound or vibration occurs, stop the operation immediately and
contact a Hitachi Koki authorized sales/service representative.

CAUTION：

●

Position　the centrifuge on a level surface, such a table or laboratory bench
that can support the weight of the centrifuge and resist vibration.

●

Be sure to remove the rotor from the rotor chamber when centrifuge is not
used for a long time or when the machine is moved. Otherwise the drive shaft
(crown) may be damaged.

●

Before using a rotor, be sure to read through the rotor instruction manual.

●

Check the chemical resistance chart attached to the rotor, and do not use any
sample inapplicable to the tubes, tube caps, bottles, or bottle caps, etc.
Using such a sample could corrode or deteriorate such parts.

●

Use the rotor tubes and bottles within their actual capacities.

●

Mount the rotor onto the drive shaft gently and properly.

●

Do not drop the rotor or apply excessive force to the drive shaft to avoid
damage to the drive shaft.

●

Maximum rotor speed depends on the tubes or adapters to be used. Follow
the instructions on the rotor instruction manual.

●

This centrifuge might convey vibration to a table while the rotor is rotating. If a
measuring device is positioned on a table and near this centrifuge, use a
measuring device carefully.

●

Approximately even quantities of sample in the tubes are sufficient for
balancing, and extremely different sample quantities must be avoided ( Refer
to Section 2-1-2 for the sample balancing).

●

Clean the inside of the drive hole (crown hole) of the rotor and the surface of
the drive shaft (crown) of the centrifuge once a month.

●

Storing the rotor on the shelf is permitted if the shelf is taken necessary
countermeasures against earthquakes not to drop the rotor.

ii

SAFETY NOTICES

CAUTION：

●

Do not pour any solution such as water, detergent, or disinfectant directly into
the rotor chamber and be careful not to spill the sample into the rotor chamber.
If you do so, the bearings of the drive unit might corrode or deteriorate and it
might cause vacuum failure.

●

Do not operate the display panel (Touchscreen) using a ball-point pen.

○Safety during installation and maintenance

DANGER：

WARNING：

CAUTION：

●

Before removing the cover, top deck, or other component for maintenance,
always turn off the POWER switch of the instrument, unplug the power cord
from the wall outlet, and wait at least three minutes to avoid the risk of
electrical shock.

●

For maintenance and repairing of the rotors, tubes, etc., see the rotors and
tubes instruction manual.

●

After installation and before any test-run, this ultracentrifuge always needs the
internal check by a Hitachi Koki authorized sales/service representative.

●

Repairs, disassembly, and other modifications to the centrifuge are strictly
prohibited unless performed by a Hitachi Koki authorized sales/service
representative.

●

If the centrifuge is exposed to ultraviolet rays for a long time, the color of the
covers may be changed or the coating may be peeled off. After use, cover
the centrifuge with a cloth to protect it from direct exposure.

○ Electrical Safety

WARNING：

CAUTION：

●

Your centrifuge must be grounded properly to avoid electrical shock hazards.

●

Do not place containers holding liquid in the rotor chamber or on or near the

instrument. If they spill, liquid may get into the instrument and damage
electrical components.

●

If the machine will not be used for a long time, turn off the main circuit breaker.

iii

SAFETY NOTICES

○Safety against Risk of Fire

WARNING：

○Chemical and Biological Safety

WARNING：

●

This centrifuge is not explosion-proof. Never use explosive or flammable
samples or materials that chemically react vigorously. Do not centrifuge such
materials in this instrument nor handle or store them near the instrument.

●

Take all necessary safety measures before using samples that are toxic or

radioactive, or blood samples that are pathogenic or infectious. You use such

samples at your own responsibility.

●

Take all necessary safety measures when Risk Group Ⅱ materials (as
identified in the World Health Organization “Laboratory Biosafety Manual”) are
handled, and that more than one level of protection shall be provided in the
case of materials of a higher group.

●

If the centrifuge, rotor, or an accessory is contaminated by samples that are
toxic or radioactive, or blood samples that are pathogenic or infectious, be
sure to decontaminate the item according to good laboratory procedures and
methods.

●

If there is a possibility that the centrifuge, rotor, or an accessory is
contaminated by samples that might impair human health (for example,
samples that are toxic or radioactive, or blood samples that are pathogenic or
infectious), it is your responsibility to sterilize or decontaminate the centrifuge,
rotor, or the accessory properly before requesting repairs from a Hitachi Koki
authorized sales/service representative.

●

It is your responsibility to sterilize and/or decontaminate the centrifuge, rotor,
or parts properly before returning them to a Hitachi Koki authorized
sales/service representative.

Notice for an Earthquake

Depending on the magnitude, an earthquake might damage centrifuge. If you observe some
abnormality, stop using the centrifuge immediately and ask for inspection by the Hitachi Koki
service representative.

iv

Table of contents

1. Description ････････････････････････････････････････････････････････････････ 1-1

1-1 External view of ultracentrifuge ･･･････････････････････････････････････････ 1-1
1-2 Structure ･･････････････････････････････････････････････････････････････ 1-2

1-2-1 Touchscreen ･･････････････････････････････････････････････････････ 1-2
1-2-2 Rotor chamber･････････････････････････････････････････････････････ 1-5
1-2-3 Safety devices ･････････････････････････････････････････････････････ 1-6

2. Operation ･････････････････････････････････････････････････････････････････ 2-1

2-1 Run preparation ････････････････････････････････････････････････････････ 2-2

2-1-1 Starting up this machine ･･･････････････････････････････････････････ 2-2
2-1-2 Preparing tubes/bottles and rotor ････････････････････････････････････ 2-3
2-1-3 Applicable rotors ･･････････････････････････････････････････････････ 2-4

2-2 Basic operation･････････････････････････････････････････････････････････ 2-5

2-2-1 Setting run conditions ･･･････････････････････････････････････････････ 2-5
2-2-2 Operational procedure ･･････････････････････････････････････････････ 2-10
2-2-3 Acceleration rates and deceleration rates･･･････････････････････････････ 2-13

2-3 How to use the option function････････････････････････････････････････････ 2-14

2-3-1 Programmed operation･･････････････････････････････････････････････ 2-15
2-3-2 Step-mode operation ･･･････････････････････････････････････････････ 2-30
2-3-3 Selecting the rotor･･････････････････････････････････････････････････ 2-41
2-3-4 Displaying and setting RCF ･･････････････････････････････････････････ 2-43
2-3-5 User login･････････････････････････････････････････････････････････ 2-47

2-4

Features of the MENU screen ･････････････････････････････････････････････ 2-49

2-4-1 Displaying the Run History and loading the information about run parameters ･･････ 2-50
2-4-2 Rotor Catalog ･････････････････････････････････････････････････････ 2-51
2-4-3 Spin-down operation････････････････････････････････････････････････ 2-52
2-4-4 RTC (real-time control) operation ･････････････････････････････････････ 2-53
2-4-5 Customizing the settings ････････････････････････････････････････････ 2-57
2-4-6 Administrator (Admin) functions･･･････････････････････････････････････ 2-60

2-5 Emergency recovery from power failure････････････････････････････････････ 2-73

3. Maintenance ･･････････････････････････････････････････････････････････････ 3-1

3-1 Rotor Chamber ･････････････････････････････････････････････････････････ 3-2
3-2 Drive Shaft (Crown) ･････････････････････････････････････････････････････ 3-2
3-3 Cabinet････････････････････････････････････････････････････････････････ 3-2
3-4 Chamber door seal ･････････････････････････････････････････････････････ 3-3

3-4-1 Removing the door seal O-ring ･･･････････････････････････････････････ 3-3

3-5 Vacuum pump･･････････････････････････････････････････････････････････ 3-4
3-6 Others ････････････････････････････････････････････････････････････････ 3-4

v

Table of contents

4. Troubleshooting ·········································································································· 4-1

4-1 Alarm indicators ······································································································ 4-2

4-2 User-corrected Problems ························································································ 4-4

5. Installation ···················································································································· 5-1

6. Warranty ······················································································································· 6-1

7. Specifications ·············································································································· 7-1

8. Supply list

····················································································································· 8-1

APPENDIX

Decontamination sheet

WEEE Compliance
Marking for Control of Pollution Caused by Electronic Information Products

(THE PEOPLE’S REPUBLIC OF CHINA)

vi

1-1 External view of ultracentrifuge

1. Description

1-1 External view of ultracentrifuge

The CS150NX Ultracentrifuge is tabletop type and requires a small amount of space.

Touchscreen (display/operation)

Door

POWER switch

Top deck

Unit: (mm)

Front cover

NOTE

582

590

External connection

* This size is the minimum size and depends on the installation conditions.

Fig. 1-1 External view of CS150NX Tabletop Micro Ultracentrifuge

*

408

1-1

1-2-1 Touchscreen

1-2 Structure

1-2-1 Touchscreen

The touchscreen with color liquid crystal screen is incorporated in the CS150NX centrifuge. You can set

the run conditions, perform the operation, and display Run History, Programmed Run, and User

Customizations Screens by pressing the screen.

Fig. 1-2-1 shows the touchscreen.

Tabs of each screen

(1) Field display

(8) USER button

The following screen appears by pressing the SPEED , TIME , or TEMP button.

(3) VACUUM
　 button/Indicator

(2) Rotor indicator

(4) START button

【Display at the normal operation】

(7) RTC button

(6) PROGRAM button

(5) STOP button

(9) Keypad display

【Display when setting the run conditions such as the speed etc.】

Fig. 1-2-1 Touchscreen

1-2

〔Functions of the Run screen〕

1-2-1 Touchscreen

No.

Name and symbol

Field display

(1)

SPEED column
(RCF column)

TIME column

TEMP column

button

Displays various fields.
The SPEED(RCF), TIME, and TEMP fields give the current status indicator in the top
row and the setting indicator in the bottom row. (For setting, see Section 2-2-1.)

 SPEED (

(Top row) Displays speeds in increments of 10 rpm at lower than 5,000 rpm, and in steps

of 100 rpm at 5,000 rpm or more.

(Bottom row) Sets speeds from 5,000 to maximum speed in increments of 1000 rpm.

For RCF , see Section 2-3-4.

 TIME

(Top row) Displays the remaining operation time. If the running time is set to

(Bottom row) Specifies a setting in the range from 1 minute to 99 hours 59 minutes in

 TEMP

(Top row) Displays in steps of 0.1 °C.
(Bottom row) Sets a setting in the range from 0 °C to 40 °C in increments of 1 °C

The column is shifted from SPEED (RCF) column to RCF (SPEED)column
by pressing this button.

The Acceleration rate and Deceleration rate are set by pressing this button.

Speed indicator)

The lower three digits (one, ten, and one-hundred positions) display zeros.

Maximum speed: 150,000 rpm

(running time indicator)

HOLD

steps of minutes and hours.

(temperature indicator)

When the pressure in the rotor chamber equals the atmospheric pressure, the

temperature of the inside of the rotor chamber is kept at 25℃ to prevent
condensation.

Functions and actions

, this field displays time elapsed.

 ACCEL (acceleration mode indicator)

button

Displays acceleration modes 1 through 9.

 DECEL (deceleration mode indicator)

Displays deceleration modes 1 through 9, along with free coast (F).

(2) Rotor indicator

(3) VACUUM button

ROTOR CATALOG is displayed by pressing this button and you can select the
desired rotor.

 Press this button to turn the vacuum pump on or off. When the vacuum pump

is turned off, the pressure in the rotor chamber will change to equal the
atmospheric pressure. (While the rotor is rotating, you can not turn off the

vacuum pump.)
 Temperature control starts as soon as the vacuum pump is turned on.
Displays the following four stages according to the vacuum of the rotor chamber.
（1） Atmospheric state.
The vacuum pump is not activated.

（2） Low vacuum. The rotor waits at 5,000 rpm until the vacuum

reaches an intermediate level.

（3） Intermediate vacuum.

（4） High vacuum.

NOTE

If the sample is sensitive to a

temperature rise, do not press
the START button until the
chamber is at a high vacuum
level.

1-3

1-2-1 Touchscreen

〔Functions of the Run screen〕

No. Name and symbol

(4)

button

(5)

button

(6)

(7)

(8)

button

button

button Displays the user name (see Section 2-4-6).

〔Functions of the keypad display〕

No. Name and symbol

(9) Keypad display

When entering the deceleration
rate, is displayed in the
position of .

FREE

Hold

〔External connection〕

Functions and actions

Starts rotor rotation. If VACUUM is off, pressing this button activates
the vacuum pump and starts temperature control.

Stops rotor rotation.

Displays the program No. if program operation has been selected.
Press this button to specify program settings (see Section 2-3-1).

Displays the start time or finish time for centrifugation. Press this
button to set a start time or finish time (see Section 2-4-4).

Use the keypad display to enter numeric values for run parameters.

：

When entering a time: Switches from hours to minutes

Hold

FREE

Press this to save the entered setting.

Enter

Press this to close the keypad display.

When entering the operation time: Sets continuous running.

When entering deceleration conditions: Sets free coasting.

Press this to cancel input (for example, if you enter the

CE

wrong number or the wrong value for a run parameter).

X

Functions and actions

No. Name and symbol Functions and actions

(10) USB (host side)

⑩

(11) USB (device side)

⑪

Use the USB connection to output the operation history of the
centrifuge to a USB flash drive.

Use for connecting optional items.

1-4

1-2-2 Rotor chamber

r

The structure of the rotor chamber (vacuum chamber) is shown in Fig. 1-2-2.

Cooling bowl (Rotor Chamber)

Cools the rotor.

Chamber door seal

1-2 Structure

Drive shaft (crown)
Sets the rotor and rotates.

Protector
(Protection steel tube)

Over-speed sensor
Stops the rotor when it rotates
faster than the maximum
allowable speed of the rotor.
(Two sensors provided)

Imbalance detection device
Detects abnormal balance and
stops the rotor.

Fig. 1-2-2 Rotor chamber

Temperature senso
Measures the temperature in the
rotor chamber.

1-5

1-2 Structure

1-2-3 Safety devices

(1) Protection of rotor chamber

Should the rotor spinning at high speed fails (or comes off the drive shaft), the safety of the operator is

ensured by the thick protector (Protection steel tube) enclosing the cooling bowl (Fig. 1-2-2).

(2) Imbalance detector

If during operation the vibration of the rotor becomes excessive due to serious imbalance or improper

bucket setting, the imbalance detector detects the situation and decelerates the rotor immediately.

However, the ultracentrifuge is designed to tolerate imbalance associated with visual balancing-it is

equipped with an imbalance tolerant drive. (For more information on the balancing of rotors, see

Section 2-1-2 "Preparing tubes/bottles and rotor".)

(3) Door lock system

The chamber door automatically locks for safety while the rotor is spinning. When the power supply is

off, the door remains locked. The door can only be opened and closed when the rotor is at rest and the

rotor chamber is vented. To open the door in the event of a power failure, see Section 2-5 "Emergency

recovery from power failure".

(4) Overspeed detector

This ultracentrifuge incorporates a detector designed to prevent the rotor from spinning at a speed

exceeding the maximum allowable speed.

Two independent microprocessors (CPUs) check the rotor for overspeed, thus increasing safety further

(dual CPU overspeed preventive mechanism).

The first CPU detects overspeed and performs control and display. Should the rotor be set to a speed

exceeding the permissible speed, this CPU detects an alarm massage of “SPEED” from the low speed

range (about 2,000 rpm), and stops the rotor.

(But this second CPU does not display an alarm message, because it is not connected to the display-

performing CPU. Should the alarm device be activated, pressing START button will not run the

instrument. Turn off the POWER switch, wait for several minutes, turn the POWER switch on again,

then pressing START button.)

1-6

Other features

A

2. Operation

2. Operation

The CS150NX Ultracentrifuge is capable of operation in more than one mode to meet a wide range of

applications. The outline of each available mode is given below:

Brief description Reference

Section 2-2,

Normal operation

Programmed operation

Speed

Time

You can store set run conditions in memory
for later use in repeated operation.

Store

Program No.

Call

Basic operation

Section 2-3-1,
Programmed operation

フ　　

Step-mode operation

ァ　　

ン　　

ク　　

シ　　

RCF (centrifugal force)
value display setting

ョ　　

ン

Speed

Time

Speed

Time

More than one normal operation can be
combined into a sequence of operations or
step for successive centrifugation.

Speed

Time

This feature calculates centrifugal force (RCF)
values from set speed.
It can also calculate reversely, i.e., finding
speed from such values.

RCF value

setting

Speed

Time

Section 2-3-2,
Step-mode operation

Section 2-3-4,
Displaying and setting
RCF

Spin-down operation

RTC (real-time control)
operation

Spin-down operation is useful to remove the
adhered samples on the interior walls of the
tubes.

Run starts or completes at a required date
and time.

Press START
button.

Speed

finish date and

time required

Time

2-1

Section 2-4-3,
Spin-down operation

Section 2-4-4,
RTC (real-time control)
operation

2-1 Run preparation

2-1 Run preparation

WARNING：1. This centrifuge is not explosion-proof. Never use explosive or flammable samples, or

materials that chemically react vigorously. Do not centrifuge such materials in this

instrument nor handle or store them near the instrument.

2. Take all necessary safety measures before using samples that are toxic or radioactive,

or blood samples that are pathogenic or infectious. You use such samples at your

own responsibility.

CAUTION ：Do not place containers holding liquid in the rotor chamber, on the centrifuge, or near the

centrifuge. If spilt, liquid might get into the instrument and damage electrical and

mechanical components.

2-1-1 Starting up this machine

Before setting run conditions, display the Run screen (Screen for setting run conditions)

(1) Displaying the Run screen (Screen for setting run conditions)

1. Turn on the POWER switch.

2. The initial screen appears.

Initial screen

3. The Run screen appears.

2-2

Run screen

Fig.2-1-1 Initial screen and Run screen

2-1 Run preparation

A

2-1-2 Preparing tubes/bottles and rotor

The CS150NX allow you to balance, by eye, tubes or bottles containing a sample solution and then centrifuge

them. Make sure that the approximate difference between meniscus levels of sample solution in tubes or

bottles is within 5 mm (See fig. 2-1-2).

Within 5mm

For an S140AT rotor, an S100AT5 rotor, and an S50

rotor, set the approximate meniscus level difference to

within 3 mm, and for an S110AT rotor and an S80AT3

rotor, set it to within 4 mm.

Tubes

Fig. 2-1-2 Balancing tubes/bottles containing a sample solution

CAUTION ： Do not run the ultracentrifuge in an extremely imbalance condition. This might cause a

mechanical failure. When balancing tubes or bottles by eye, alarm message

“IMBALANCE” might appear. Balance tubes or bottles more accurately again if alarm

message “IMBALANCE” appears.

Depending on the type of tube or rotor combined with this machine, an excessively low liquid level　may limit

the speed or crush the tube.

• When sealed tubes are used, fill the tubes with liquid to the full level.

CAUTION ： 1. Before using a rotor, read the rotor instruction manual carefully.

2. Do not use any corroded, scratched, cracked, or otherwise damaged rotor or bucket.

Before operation, always check if there is no corrosion and damage on the rotor

surface.

3. Before running a swing rotor, make sure that each bucket is hooked on a pin securely.

Poor setting might seriously damage the instrument. Even though all of them are not

used, make sure to install all buckets at any time. Make sure that all the rotor buckets

are of the same type.

4. Some tubes and adapters are inapplicable at the maximum speed of the rotor.

Refer to the rotor instruction manual carefully.

5. The S58A rotor, S55A rotor, and S50A rotor can handle sample whose mean density is

1.2 g/ml or less.

Do not rotate this rotor at its maximum speed when using a sample whose mean

density is over 1.2 g/ml. Refer to the rotor instruction manual (Part No. S999276) for

how to limit the maximum speed when using a sample whose mean density is

over 1.2 g/ml.

2-3

2-1 Run preparation

2-1-3 Applicable rotors

Rotors applicable to the CS150NX are limited to the quick setting type illustrated below. A quick setting rotor

can be installed by merely placing it on the drive shaft (crown) in the rotor chamber. Screw-type rotors used in

older models (CP120H/CP100H, CS120/CS100, CS120EX/CS100EX, and CS120FX/CS100FX) are

inapplicable to the CS150NX.

Rotor set handle

Cap (blue)

(without a screw hole)

Cover (black)

Quick setting type

(1) Applicable rotor

CAUTION ： 1. Always use a quick setting rotor for this ultracentrifuge. The screw-type rotors are

inapplicable.

Quick-setting rotors are not applicable in older models (CP120H/CP100H,

CS120/CS100, CS120EX/CS100EX, and CS120FX/CS100FX).

2. Install the rotor carefully and securely on the drive shaft in the rotor chamber. Forcefully

moving the shaft sideways or dropping the rotor onto the drive shaft may damage the

shaft.

3. The S52ST swinging bucket rotor and S50ST swinging bucket rotor are specifically

designed for the CS150NX, CS-GXⅡseries, and CS-GXL series ultracentrifuges.

These rotors cannot be used in any other centrifuge.

Screw – type for older models

(2) Inapplicable

Screw cap (with a screw hole)

Cover (blue)

2-4

2-2 Basic operation

2-2 Basic operation

WARNING: Do not incline or move the instrument while the rotor is spinning.

Do not place any object on the instrument or lean on the instrument.

CAUTION: 1. Do not press the touchscreen with a sharp-pointed object such as a ballpoint pen.

2. If abnormal sound is heard during the operation, stop the operation immediately

and contact a Hitachi Koki authorized sales/service representative.

2-2-1 Setting run conditions

This section will first describe the screen for basic operation (the Run screen). For the display at the normal

operation and the display when setting the run conditions, refer to Section 1-2-1.

[Run Screen]

The screen for displaying run conditions and operational status is called the Run screen.

Speed, time, and temperature are displayed in two rows: the top row displays the current actual run

conditions, while the bottom row displays the set run conditions.

The acceleration (ACCEL) and the deceleration (DECEL) fields display set conditions.

Operation state
display field

Setting value
display field

Fig. 2-2-1 Run screen

2-5

2-2 Basic operation

y

[Display and operations when entering the run parameters]

The keypad display appears by pressing the SPEED column, TIME column, button.

ACCEL:
DECEL:

(1) Press the column of the

desired item to turn the
first digit blue.

Color of the first digit：Blue

(2) Enter the desired numeric value

with the keypad display.
(e.g.) SPEED:150,000rpm

Press the .

1 5 0

(3) If you do not want to change

any other setting, press the

button on the

Enter

keypad display.

If you do want to change

other setting, press the

column of the desired item,

and the pressed column will

become the input wait state.

The new setting is displayed

on the setting value displa

field.

Keypad display]

[

Setting value display field

Fig. 2-2-2 Setting value display field

2-6

2-2 Basic operation

The next page describes how to set run condition by citing some examples.

NOTE (1) If you enter the wrong value, press the CE button and then enter the correct value.

If you have pressed the Enter button, repeat step (1) of the previous page, and then enter

the correct value.

(2) When setting the two or more of three run conditions (SPEED, TIME, and TEMP), you do not

have to press the Enter button after each setting. You can store the setting by pressing the

desired item.

(3) If the system is running in (HOLD) and you want to set it to shut down at a future time, enter a

new time setting while the instrument is in operation; enter the sum of the time elapsed plus the

time remaining. If, for example, this machine has run continuously for five hours and you want

to stop it one and a half hours later, press the TIME column, then enter

.

6

:

3

0

2-7

2-2 Basic operation

e

y

s

f

：

• How to set speed, running time, temperature, and other parameters

Here are some examples and descriptions:

Setting item

Typical setting 150,000rpm 2 hours 30 minutes 4°C

1 Press the SPEED,TIME,

or TEMP column to show
the keypad display.
The last entered value

2

is displayed on each
column. Press the
column of the desired
item again if the color
of the first digit is
white.
Go to procedure 3 if
the color of the first
digit is blue.

3 Enter the desired numeric

value with the keypad
display.

7 8 9

4 5 6

1 2 3

0

Operation procedure

Entered numbers ar
moved to the left ever
time a new number i
entered.

Hold

Enter

RPM

(SPEED)

Color:Blue

1 5 0 2

The last three digits

are fixed.

CE

×

：

Running time

（TIME）

Color:Blue

3 0

Press the

：

button to turn the
number of minutes
blue.

Temperature

(TEMP)

Color:Blue

4

For a continuous
run, press the

Hold

button.

Make a check, then if

4

you want to change
other setting, press

Set it to 150,000rpm.

Set it to 2：30.

(2 hours 30 minutes)

Set it to 4

°C

the column of the
desired item. If you do
not want to change
any other setting,
press the

Enter

button.
Use the

CE

button to cancel input.

Can be set to any value
in the range from 0 to
40 °C in increments o
1 °C.

Setting range and units

Can be set to any value in
the range from 5,000 rpm
to maximum speed
in increments of 1,000 rpm.

Can be set to any value up
to 99 hours 59 minutes in
increments of 1 minute.

2-8

2-2 Basic operation

Setting item

Acceleration

(ACCEL)

Typical setting 9 7

1

Press the button

ACCEL:5
DECEL:5

to show the keypad display.

Press the column of the

2

desired item.

Enter the desired numeric

3

value with the keypad display.

9 7

Deceleration

(DECEL)

Operation procedure

5

7 8 9

4 5 6

1 2 3

0

Make a check, then if you
want to change other
setting, press the column
of the desired item. If you
do not want to change any
other setting, press the
button.

Enter
Use the
button to cancel input.

Setting range and units

CE

CE

FREE

Enter

×

：

For free coast,

press the button.

FREE

Set it to 9. Set it to 7.

7

1-9 1-9

+

free coast（FREE）

2-9

2-2-2 Operational procedure

2-2-2 Operational procedure

Given below is a description of the operational procedure for a normal run.

Before starting up this machine, carefully read the operation manual for your rotor and make sure

NOTE

that you have selected the correct type of tubes and entered the correct amount of sample.

Process Touch panel operation Screen display and notice

1 Turn on the POWER switch on

this machine.

2 Install the rotor. (the quick

setting rotor.)

3 Set run conditions.

Press the VACUUM button.

4

(You can omit this step.)

 The touchscreen is displayed.
 The door is unlocked.

 Install the rotor securely on the crown. (It does not have to be

screwed into the crown.)

 Always use a quick setting rotor (see Section 2-1-3

“Applicable rotors”).

 See Section 2-2-1 “Setting run conditions” and set run

conditions.

 The machine starts evacuating the rotor chamber.
 Temperature control starts.
 The degree of vacuum in the rotor chamber is displayed on

the vacuum indicator on the VACUUM button.

(1) In a low vacuum

(1 indicator)

(2) In an

intermediate vacuum

(2 indicator)

(3) In a high vacuum

(3 indicator)

Press the START button

5

 If the rotor compartment has moisture or frost on it, it takes a

long time to reach an intermediate high vacuum. In that case,
wipe it off with a clean, dry cloth or sponge.

 If the sample is sensitive to a temperature rise, do not press

the START button until the chamber is at high vacuum level.

The START button blinks and the rotor starts turning.



.

 The timer begins operating. (When the actual run timer is

activated, the timer begins operating after the speed reaches
the set speed.)

 When the speed reaches the set speed, the START

button lights up.

 This ultracentrifuge waits at 5,000 rpm until an intermediate

vacuum is reached.

2-10

Process Touch panel operation Screen display and notice

The specified centrifugation

6

time elapses (time-out).
Or press the STOP button.

The rotor stops.

7

8

Press the VACUUM button.

 The STOP button blinks and the rotor starts decelerating.

 The STOP button lights up.

 The stop signal sounds to indicate that the rotor has stopped.
 The vacuum stops, the air leak valve activates, and the rotor

chamber reaches atmospheric pressure.

 The door unlocks, and is able to be opened and closed.

2-2-2 Operational procedure

Take out the rotor.

9

 Stop the rotor completely before taking it out.

NOTE : When the rotor chamber is vacuumed insufficiently before starting operation or the ambient

temperature is low (10°C or below), the vacuum waiting time at 5,000rpm may become longer. Also,

during acceleration up to the set speed, the instrument may become a vacuum waiting state.

Therefore, before starting operation, vacuum the rotor chamber sufficiently (approx. 15 minutes)

and then press the VACUUM button and check that the displayed vacuum is at a high vacuum

level (3 indicator).

2-11

2-2-2 Operational procedure

r

r

A

r

Fig. 2-2-3 summarizes the operational procedure.

START

Turn on the POWER switch.。

Open the chamber doo

and install the rotor.。

Close the door and set

the run conditions.。

Press the VACUUM

*

button.

Press the START
button.

For information on how to
set the run conditions,
see Section 2-2-1.

(1) Vacuum pump is ON.

Temperature control starts.
(2) If the sample in the rotor is
sensitive to a temperature rise,
wait until the rotor chamber is

at high vacuum level.

The rotor is rotating at set speed.

Reaches the set time o
press the STOP button.。

Rotor stops.

Press the VACUUM
button.

Both of the vacuum pump and
temperature control turn off.

ir vent opens and the doo

lock is released.

Open the chamber door,
remove the rotor, then turn
ultracentrifuge power off.。

End

*This step may be omitted, in which case pressing the START

button later in the procedure turns on the vacuum pump and the
rotor stays at 5000 rpm to wait for chamber vacuum to reach its
intermediate level.

Fig. 2-2-3 Operational procedure

2-12

2-2-3 Acceleration rate and deceleration rate

r

f

2-2-3 Acceleration rate and deceleration rate

In order to meet various experimental protocols, the acceleration and deceleration rates can be adjusted.
The figure and table below show the relationship between ACCEL/DECEL code numbers selected and
resulting approximate acceleration/deceleration times.

Speed (rpm)

1 - 9 : ACCEL/DECEL code numbers

(No Brake)

Time

Code

No.

9
8
7
6
5
4
3
2
1
F

Acceleration time

(minutes)

from rest to 5000 rpm

Minimum time*

0.5
1

1.5
2

2.5
3

3.5
4

-

*: The minimum time is the one that occurs

when the rotor is being accelerated o
decelerated with the　maximum torque o
the driving motor. This time varies with
the type of rotor in use.

Deceleration time

(minutes)

from 5000 rpm to rest

Minimum time*

Coasting

deceleration

1
2
3
4
5
6
7
8

NOTE: The times for acceleration and deceleration may be longer than the values listed above,

depending upon the type of rotor in use.

Typical examples of application of acceleration and deceleration rates

Code No.

Type of centrifugation

Density gradient centrifugation using a
vertical rotor

DNA separation by CsCl isopycnic
separation (self-forming gradients)

Pelleting using a fixed angle rotor

Density gradient centrifugation using a
swinging bucket rotor

ACCEL DECEL

57

97

99

5 to 8 5 to 8

The sample and gradient in tubes reorient during
acceleration and deceleration. Therefore, the
sample and gradient can become mixed, especially in
wide tubes, if you use rapid acceleration or
deceleration.
You can operate at maximum acceleration because
the density gradient is not formed during the run. As
for the deceleration, it is better to decelerate slowly to
obtain sharp bands.
Rapid pelleting of samples is possible (the run time
decreases).
The sample and gradient do not reorient. Therefore,
mixing of the layers is less than that in the case of
using a vertical rotor. But it is safe not to accelerate
or decelerate the rotor by selecting minimum time.

Characteristic of separation

2-13

2-3 How to use the OPTIONS function

2-3 How to use the option function

This ultracentrifuge incorporates a number of features, such as step-mode and other programmed running,
display and setting of centrifugal force, and RTC (real-time control) that can run the centrifuge at a required
date and time. Buttons for these features are displayed and specified on the Run screen.

RTC button

SPEED/RCF button

PROGRAM

:

button

SPEED/RCF

:

button

Fig. 2-3-1 Option buttons

Programs, stores, and recalls run conditions.
This feature also offers a step-mode operation:
a continuous run of multiple run conditions.

This feature is used to cause the system to automatically
calculate and display an RCF value. It also sets an RCF
value and calculates the speed.
RCFmax indicates the maximum centrifugal force for the
maximum radius Rmax of the rotor which is used.
RCFavg indicates the average centrifugal force for the
average radius

Ravg of the rotor which is used.

PROGRAM button

Ravg

Rmax

RTC button

:

Sets a start time or a finish time and runs

Fig. 2-3-2 Rotor radius

the ultracentrifuge at a desired date and time.

The above features can be used in combination.

NOTE

To perform a combination of PROGRAM and RTC , first set PROGRAM and then set the

RTC .

Once RTC is activated, you cannot change the run time. You therefore cannot activate

PROGRAM .

2-14

2-3-1 Programmed operation

2-3-1 Programmed operation

When a centrifugal condition is to be used frequently, entering the same condition every time you want to

perform centrifugation is inconvenient.

This ultracentrifuge has a programmed operation feature that stores run conditions. Storing run conditions

which you often use allows you to call those conditions however often you may wish, thus saving time in

setting. (Even while the POWER switch is OFF, this centrifuge retains the conditions entered.)

This centrifuge incorporates the program areas indicated below. It has twenty memory areas and nine steps

in each memory unit.

Running this machine with each memory unit retaining multiple steps will allow you to change the speed, run

time, temperature, and others while in operation.

(Step-mode operation)

Memory 1 Step 1 Step 2 ······ Step 9

Memory 2 Step 1 Step 2 ······ Step 9

Memory 3 Step 1 Step 2 ······ Step 9

·

·

·

·

·

Memory 20 Step 1 Step 2 ······ Step 9

·

·

·

·

·

Fig. 2-3-3 Program areas

·

·

·

·

·

······

······

······

······

······

·

·

·

·

·

2-15

2-3-1 Programmed operation

p

r

(Basic operation of the programmed operation feature)

Press the PROGRAM

button.

Program No. which stores the

arameters is displayed.

run

If you create, change, o

delete a program, see the

below.

If you create or change a program,

If you delete a program,

If you load the run parameters,

into the centrifuge, see the

below.

Programmed operation possible

Press the Run button

to go back to the Run

screen. The loaded run

parameters are displayed on

the Run screen.

Press the Select button.

Press the Delete button.

*

Press the column of the

desired Program No

.

NOTE You cannot create, change, or delete a program while in running. Perform these operations while not in

running. However, you can search the PROGRAM screen every time.

*If you do not know Program No. of the program which you need, see Section 2-3-1 (2) (b).

2-16

(1) Programming procedure for run conditions (creating or changing)

Shown below is the procedure for storing (creating) or changing an run condition.

If you want to store the user name in the program, you have to load the user name into the centrifuge

in advance (see Section 2-4-6 (1)).

Process Touch panel operation Screen display and notice

Press the PROGRAM

1

button on the Run screen.

2-3-1 Programmed operation

PROGRAM button

● PROGRAM screen

appears.

● If Program name and Parameter are displayed in the

column of the Program No., it indicates that the run
parameters has been already stored in this program.

● If you want to create the program, press the column where

Program name and Parameter have not been displayed.

2-17

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Press the column of the

2

program No. that you want to
create (change) the program.
Check that the pressed column
turns blue and press the

Select button.

If you want to store
the run parameters
as Program No.4.,
press the column
of the Program
No.4.

Select button

If you want to change the
contents of the program,
change the desired item (see
the below).

Procedures for entering the

3

program name are described
as follows.

a) Press the column of the

program name in the
PROGRAM screen.

b) Enter the program name in

the Program name screen.

c) Press the Enter button.

● The PROGRAM

screen　appears.

Program name
column

User name column

Rotor column

a) The Program name

screen appears.

b) If you enter the

“DNA”,

the “DNA” is

displayed on the

column of the

program name.

2-18

c) The PROGRAM

screen returns
to view.

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Procedures for selecting the

4

user name are described as
follows.

a)The PROGRAM

User name
screen appears.

a）Press the column of the

user name in the
PROGRAM screen.

b）Select the user name and

press the column of the
selected user name.

Then press the Enter button.

b）The PROGRAM

screen returns

to view.

If you selected the

“Hitachi-Koki”,

the “Hitachi-Koki”

is displayed on

the column of the

user name.

2-19

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Procedures for selecting the

5

rotor are described as follows.

a）Press the column of the rotor

in the PROGRAM screen.

b）Press the Rotor Catalog

button in the PROGRAM
Rotor Management screen.

 You can select your rotor in the PROGRAM Rotor

Management screen, if you registered your rotor in
advance (see Section 2-4-6 (5) “Rotor Management”).

a） The PROGRAM

Rotor
Management
screen appears.

c）Select the rotor in the

PROGRAM ROTOR
CATALOG screen and press
the column of the selected
rotor.

Then press the Enter button.

b）The PROGRAM

ROTOR CATALOG

screen appears.

c）The PROGRAM

screen returns

to view.

If you selected the

“S150AT”,

the “S150AT” is

displayed on

the column of the

Rotor.

2-20

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Procedures for selecting the

6

run parameters are described
as follows.

［Entering the SPEED value］
If you want to enter the SPEED
value as the parameter,
press the Edit button
after checking that the color of
the SPEED column is blue.

SPEED column

Edit button

● The Enter

PROGRAM

screen appears.

［Entering the RCF value］
If you want to enter the RCF
value as the parameter, press
the RCFmax (RCFavg)
column.
Press the Edit button
after checking that the color of
the RCFmax (RCFavg) column
turns blue.

RCFmax column RCFavg column

Edit button

2-21

A

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Procedures for entering the run

7

parameters are described as
follows.

a）Press the Edit button

after checking that the color
of the STEP1 column is
blue. Enter the run
parameters on the keypad
display (see Section 2-1-2).
If you want to enter the
acceleration and
deceleration rate, press the

ACCEL/DECEL button

OK button

ACCEL/DECEL button.
.

Example:
SPEED:150,000 rpm
TIME:2hr.30min.
TEMP:20°C

CCEL:9

DECEL:9

b) Press the OK button

after entering the run
parameters. (If you want to
change the run parameters,
press the column of the

Run button

Edit button
desired STEP. Press the
Edit button after checking
that the pressed column is
blue and enter the run
parameters.)

 To enter the run parameters in multiple steps in a step-

mode operation, continue the operation from the above
(see Section 2-3-2 “Step-mode operation”).

 You can not skip a step. (You can not enter anything in

STEP 2 with nothing entered into STEP1.)

a) The keypad

display is shown.

b)The entered run

parameters are
displayed in the
Enter
PROGRAM
screen.

8

Press the Run button in
the PROGRAM screen.

● The Run screen

appears.

Program name

NOTE (1) If you make and store changes in a column that already stores run parameters, the previous

parameters are replaced by the new parameters.

(2) You cannot store a run parameter while in running (while the rotor is rotating). Always perform

this function while not in running.

2-22

2-3-1 Programmed operation

(2) How to perform a programmed operation

Shown below is how to perform a “programmed operation”, that is, how to call a stored set of run
parameters and run this centrifuge accordingly.

(a) If you know the program name you need

Process Touch panel operation Screen display and notice

Turn on the POWER switch on

1

the centrifuge.

Install a rotor.  Install the rotor securely on the shaft. (The rotor does not

2

 Touchscreen appears.
 The door unlocks.

have to be screwed into the shaft.)

 Always use a quick setting rotor (see Section 2-1-3

“Applicable rotors.”)

3

Press the PROGRAM button
on the Run screen.

PROGRAM button

● The PROGRAM

screen appears.

2-23

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Press the column of the

4

desired program name and
press the Run button
after checking that the pressed
column turns blue.

Run button

STEP：1-3

In this case, this program
contains three steps.

In the Run screen, the run
parameters for the first step in
this program are displayed.

Run the centrifuge under

5

normal operation without
making changes to the run
parameters.

Program name

 The program name is displayed on the PROGRAM

button indicator.

 If the “STEP:1-3” is displayed on the PROGRAM

button indicator, this means that the program you have
just called stores multiple run parameters (step-mode
feature). For details, see Section 2-3-2 “Step-mode
operation”.

 Run this centrifuge according to Section 2-2-2 “Operation

procedure”.

 If you make changes to the run parameters (such as

SPEED and TIME) after calling a program, the program
you have just called is canceled. You must call it again
to use it.

2-24

(b) If you do not know the program name you need

Process Touch panel operation Screen display and notice

2-3-1 Programmed operation

Turn on the POWER switch on

1

the centrifuge.

Install a rotor.  Install the rotor securely on the shaft. (The rotor does not

2

 Touchscreen appears.
 The door unlocks.

have to be screwed into the shaft.)

 Always use a quick setting rotor (see Section 2-1-3

“Applicable rotors.”)

3

Press the PROGRAM
button on the Run screen.

PROGRAM button

● The PROGRAM

screen appears.

2-25

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Press the column of the

4

desired program name whose
contents you want to check.
Then press the Select

button after checking that the
pressed column turns blue.

Check the user name and rotor
name in the Program screen
and decide whether this
selected program is the
program that you need or not.

Select button

If you think that the selected
program is the program you

need, press the Edit
button to check the run

parameters.
If you do not think that the

selected program is the
program you need, press the

x button to switch to the
previous screen.

Edit button

After checking the run
parameters, press the

Run button if the selected
program is the program you
need.

STEP：1-3

In this case, this program has
three steps.

In the Run screen, the run
parameters for the first step in
this program are displayed.

Run button

Program name

 The program

name is
displayed on the
PROGRAM

button indicator.

 If the “STEP:1-3” is
displayed on the

RROGRAM

button indicator, this
means that the
program you have
just called stores
multiple run
parameters (step­mode feature).
For details,
see Section
2-3-2 “Step-mode
operation”.

2-26

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Run the centrifuge under

5

normal operation without
making changes to the run
parameters.

 Run this centrifuge according to Section 2-2-2 “Operation

procedure”.

 If you make changes to the run parameters (such as

SPEED and TIME) after calling a program, the program
you have just called is canceled. You must call it again to
use it.

NOTE (1) To perform a combination of a programmed run with RTC (real-time control) (see Section 2-4-

4 “RTC operation”), call a programmed memory unit, then set RTC. The system will then

calculate the total of the running times of all steps of the programmed run and calculate the

start time for RTC. Therefore, cannot call the program memory after setting RTC.

2-27

x

2-3-1 Programmed operation

(3) Deleting a program

This section explains how to delete a program.
To delete a program, delete all steps in that program.

You cannot delete a program while in running (while the rotor is rotating). Always perform this

NOTE

function while not in running.

Process Touch panel operation Screen display and notice

1 Press the PROGRAM button

on the Run screen.

PROGRAM button

Press the column of the

2

program name which you
want to delete. Then press the

Delete button after
checking that the pressed
column turns blue.

● The PROGRAM

screen appears.

● The deletion

confirmation bo

is displayed.

2-28

2-3-1 Programmed operation

Process Touch panel operation Screen display and notice

Press the Yes button on

3

● The program is

the deletion confirmation box.

deleted.

2-29

2-3-2 Step-mode operation

2-3-2 Step-mode operation

This ultracentrifuge incorporates a step-mode operation feature, which stores two or more run conditions in

one program memory area and switches between different values of speed, running time, temperature, and

other parameters while in operation. This centrifuge can store up to nine steps.

This section explains how to make settings by citing some examples.

(1) How to activate a step-mode operation

[Typical settings]

Shown below is the example of a three-step run and how to activate a step-mode operation.

Step 1 Step 2 Step 3

Speed 150,000 rpm 120,000 rpm 100,000 rpm

Run time 1 h 30 min 1 h 30 min

Temperature 20°C 20°C 20°C

Acceleration mode 9 9 9

Deceleration mode 9 9 7

Step 1

150,000

Step 2

120,000

Step 3

100,000

Speed (rpm)

1 h 30 min

1 h

30 min

Run time

Fig. 2-3-2 A typical step-mode run

2-30

2-3-2 Step-mode operation

Process Touch panel operation Screen display and notice

Press the PROGRAM

1

button on the Run screen.

PROGRAM button

● The PROGRAM

screen appears.

● If a Program name and Parameter are displayed in the

column of a Program No., run parameters have already
been stored for that program.

● If you want to create a new program, press a column that

does not display a Program name or Parameter.

2-31

2-3-2 Step-mode operation

Process Touch panel operation Screen display and notice

Press the column of the

2

program No. for which you
want to create (or change) a
program, and make sure that
the pressed column turns blue.

Then press the Select
button.

If you want to store
the run parameters
as Program No.4.,
press the column
of the Program
No.4.

Select button

● The PROGRAM

screen　appears.

Enter a program name as

3

follows:

a) Press the column of the

program name in the
PROGRAM screen.

b) Enter the program name in

the Program name screen.

c) Press the Enter button.

Program name
column

User name column

Rotor column

a)The Program name

screen appears.

b)If you enter the

“DNA-STEP”,

these characters

are displayed on

the column of the

program name.

c)The PROGRAM

screen reappears.

2-32

2-3-2 Step-mode operation

Process Touch panel operation Screen display and notice

Select a user name as follows:

4

a) The PROGRAM

User name

a）Press the column of

screen appears.

User name in the
PROGRAM screen.

b）Press the column of the user

name you want to select.

Then press the Enter button.

b）The PROGRAM

screen reappears.

If you selected the

“Hitachi-Koki”,

these characters

are displayed on

the column of the

user name.

2-33

2-3-2 Step-mode operation

Process Touch panel operation Screen display and notice

Select a rotor as follows:

5

a）Press the column of the rotor

in the PROGRAM screen.

b）Press the Rotor Catalog

button in the PROGRAM
Rotor Management screen.

 If you registered your rotor in advance (see Section 2-4-6

(5) “Rotor Management”), you can select it in this
PROGRAM Rotor Management screen,

a） The Program

Rotor
Management
screen appears.

c）In the PROGRAM ROTOR

CATALOG screen, press the
column of the rotor you want
to select.

Then press the Enter button.

b）The PROGRAM

ROTOR CATALOG

screen appears.

c）The PROGRAM

screen reappears.

If you selected the

“S150AT”,

these characters

are displayed on

the column of the

Rotor.

2-34

2-3-2 Step-mode operation

Process Touch panel operation Screen display and notice

Select run parameters as

6

follows:

［To enter a SPEED value］
If you want to enter a SPEED
value as a parameter, press
the SPEED column and make
sure that it turns blue.
Then press the Edit
button.

SPEED column

Edit button

● The Enter

PROGRAM

screen appears.

［To enter a RCF value］
If you want to enter a RCF
value as a parameter, press
the RCFmax (or RCFavg)
column and make sure that it
turns blue.
Then press the Edit
button.

RCFmax column RCFavg column

Edit button

2-35

A

2-3-2 Step-mode operation

A

A

Process Touch panel operation Screen display and notice

Enter run parameters as

7

follows:
a）Make sure that the STEP 1

column is blue, and then
press the Edit button.
Enter the run parameters for
the STEP 1 on the keypad
display (see Section 2-1-2).
If you want to enter an
acceleration or deceleration
rate, press the
ACCEL/DECEL button.

Run parameters of the STEP 1:
SPEED: 150,000 rpm
TIME: 1hr.30min.
TEMP: 20°C

CCEL: 9

DECEL: 9

ACCEL/DECEL button

OK button

b) After entering the run

parameters, press the OK
button (If you want to
change run parameters,
press the column of the
desired STEP, and make
sure that it turns blue. Then
press the Edit button.

c) Enter the run parameters for

STEP 2 on the keypad

Run button

 You can not skip a step. (If you enter nothing in STEP 1,

you cannot enter anything in STEP 2.)

Edit button

display in a similar way.

Run parameters of the STEP 2:
SPEED: 120,000 rpm
TIME: 1hr.
TEMP: 20°C

CCEL: 9

DECEL: 9

d) Enter the run parameters for

the STEP 3 on the keypad
display in a similar way.

Run parameters of the STEP 3:
SPEED: 100,000 rpm
TIME: 30min.
TEMP: 20°C

CCEL: 9

DECEL: 9

a) The keypad

display appears.

b)The run

parameters

entered for

STEP 1 are

displayed in the

Enter PROGRAM

screen.

c)The run

parameters
entered for
STEP 2 are
displayed in the
Enter PROGRAM
screen.

d)The run

parameters
entered for
STEP 3 are
displayed in the
Enter PROGRAM
screen.

2-36

Process Touch panel operation Screen display and notice

8

Press the Run button in the

● The Run screen

PROGRAM screen.

Run button

2-3-2 Step-mode operation

appears.

Run the centrifuge under

9

normal operation without
changing to the run
parameters.

Program name

 Run the centrifuge according to Section 2-2-2 “Operation

procedure”.

 If you change to a run parameters (such as SPEED or

TIME) after calling a program, the program you have just
called is canceled. You must call it again to use it.

 Pressing the START button rotates the rotor under the run

parameters of STEP 1.

 When STEP 1 ends, the system moves to STEP 2 and

STEP 3 automatically.

2-37

2-3-2 Step-mode operation

NOTE (1) Step display

The PROGRAM button indicator displays steps as follows:

In this case, this program contains three steps.
In the Run screen, the run parameters for the first step in this program are displayed.

When the system finishes separating step 1 and moves to step 2,

STEP:1-3

STEP:2-3

When the system moves from step 2 to the final step 3,

STEP:3-3

Thus, you can see at a glance how many steps are stored in the specified memory and
which step the centrifuge is following.

(2) You cannot store a run parameter while in running (while the rotor is rotating). Always perform

this function while not in running.

(3) To perform a combination of a step mode run with an RTC (real-time control) run (see Section 2-

4-4 “RTC (real-time control) operation”), call a program memory unit, then set RTC.

The system then calculates the total of running times of all steps of the programmed run and

calculates the startup time for RTC. You therefore cannot call a program memory unit after setting

RTC.

2-38

(3) Deleting a program

x

This section explains how to delete a program.
To delete a program, delete all steps in that program.

You cannot delete a program while in running (while the rotor is rotating). Always perform this

NOTE

function while not in running.

Process Touch panel operation Screen display and notice

1 Press the PROGRAM button

on the Run screen.

2-3-2 Step-mode operation

PROGRAM button

Press the column of the

2

program name which you
want to delete. Then press the

Delete button after
checking that the pressed
column turns blue.

Press the Yes button on

3

the deletion confirmation box.

● The PROGRAM

screen appears.

● The deletion

confirmation bo

is displayed.

● The program is

deleted.

2-39

2-3-2 Step-mode operation

(3) Other procedures

1) Making changes to the run parameters
Call the memory unit you want to make changes to and make the changes. Alternatively, delete the
memory unit, then store a new set of run parameters.
To make a change that will result in fewer steps, delete the memory unit and then enter a new set of
run conditions and store them.

2) Running the centrifuge starting from an intermediate step
You cannot run the centrifuge starting from an intermediate step in a memory unit that contains multiple
steps.
Store (register) run parameters for the intermediate step and later steps in another memory unit.
Then call the memory unit and run it.

3) What if a SPEED alarm goes on?
If a step stores a speed exceeding the maximum allowable speed of your rotor, the system will detect it
in the STEP1 run and display the SPEED alarm.
Double-check the speed of all steps and correct any wrong ones.

4) Stopping the centrifuge in operation
Press STOP button. The rotor stops and the system does not move to the next step.

2-40

2-3-3 Selecting the roto

r

r

r

2-3-3 Selecting the rotor

This ultracentrifuge stores the maximum and average radii of each rotor in the internal memory. Setting a
speed causes this centrifuge to automatically calculate and display the RCF (relative centrifugal force)
value, while setting an RCF value causes the centrifuge to automatically calculate and display the speed.
You can control the rotor data such as total operation hours and number of runs by registering your
rotors in the centrifuge.

(1) Procedure for selecting the rotor

Process Touch panel operation Screen display and notice

Check that the desired rotor is

1

displayed in the ROTOR
indicator of the RUN SCREEN.
When the desired rotor is not
displayed, pess the ROTOR
indicator.

ROTOR
indicato

Check whether the desired

2

rotor is displayed in the Rotor
Management screen or not.
When the desired rotor is
displayed, press the column of
the desired rotor and press the
OK button. When the desired
rotor is not displayed, press
the Rotor Catarog

button.

Press the column of the

3

desired rotor on the ROTOR
CATALOG screen. Check that
the pressed column turns blue
and press the Enter

button.

● The Rotor

Management
screen appears.

Nothing is
displayed in the
ROTOR indicate
by pressing
the Cancel
button.

○ You can select your rotor in the Rotor Management

screen, if you registered your rotor in advance (see
Section 2-4-6 (5) “Rotor Management”).

● The ROTOR

CATALOG screen
appears.

The pressed
column turns
from white to
blue.

2-41

r

2-3-3 Selecting the rotor

Process Touch panel operation Screen display and notice

The desired rotor is displayed

4

in the ROTOR indicator of the
Run screen.

● The Run screen

returns to view.

ROTOR
indicato

NOTE (1) Selecting the rotor is required to display and set an RCF value (see Section 2-3-4 “Displaying

and setting RCF”).

(2) You can select your rotor in the Rotor Management screen, if you registered your rotor in

advance (see Section 2-4-6 (5) “Rotor Management”).

(3) Selecting the rotor is required when the rotor lockout function is enabled (see Section 2-4-6 (3)

“Rotor Lockout”).

2-42

r

2-3-4 Displaying and seting RCF

2-3-4 Displaying and setting RCF

This ultracentrifuge stores the maximum and average radii of each rotor in the internal memory. Setting a
speed causes this centrifuge to automatically calculate and display the RCF (relative centrifugal force)
value, while setting an RCF value causes the centrifuge to automatically calculate and display the speed.
Given below is a description of how to display and set RCF.

(1) How to display an RCF value

Process Touch panel operation Screen display and notice

Check that the desired rotor is

1

displayed in the ROTOR
indicator of the Run screen.
Go to step 3 when the desired
rotor is displayed. When the
desired rotor is not displayed,
pess the ROTOR indicator to
show the ROTOR CATALOG
screen.

ROTOR
indicato

Press the column of the rotor

2

you want to see the RCF
value. The column turns from
white to blue. Check that the
pressed column turns blue
and press the Enter
button.

The pressed
column turns
from white
to blue.

2-43

2-3-4 Displaying and seting RCF

Process Touch panel operation Screen display and notice

3

When RCFmax or RCFavg is
displayed in the column,
touch the 　　 button to

SPEED

RCF

show SPEED in the SPEED
column. Press the SPEED
column and enter the SPEED
value you want to convert into the
RCF value with the keypad
display.

(e.g.) Rotor: S150AT

SPEED: 100,000 rpm
Press
, and press the

0

1

Enter button to enter the

setting.

 See Section 2-2-1 “Setting run conditions.”

SPEED column

0

4 Press the button to

SPEED

RCF

turn the SPEED column to the
RCFmax column or the
RCFavg column.

 The RCF value

of the rotor
displayed in
the ROTOR
indicator is
displayed on
the setting value
display field of
the RCF column.

This is the RCF
value for the
SPEED value
entered in step 3.

RCFmax column: The maximum RCF value for the rotor set

speed is displayed.

RCFavg column: The average RCF value for the rotor set

speed is displayed.

2-44

r

2-3-4 Displaying and seting RCF

(2) How to set an RCF value

Select the desired rotor and enter an RCF value, and the centrifuge will calculate, set, and display the
speed. Shown below is the procedure.

Process Touch panel operation Screen display and notice

Check that the desired rotor is

1

displayed in the ROTOR
indicator of the Run screen.
Go to step 3 when the desired
rotor is displayed. When the
desired rotor is not displayed,
press the ROTOR column to

ROTOR

indicato
show the ROTOR
CATALOG screen.

Press the column of the rotor

2

you want to see the RCF
value. The column turns from
white to blue. Check that the
pressed column turns blue and
press the Enter button.

The pressed
column turns
from white
to blue.

3

When “SPEED” is displayed in
the column, press the

SPEED

　　 button to show

RCF

“RCFmax” (“RCFavg”) in the
RCFmax (RCFavg) column.
Press the RCFmax (RCFavg)
column and enter the SPEED
value you want to convert into
the SPEED value with the
keypad display.

(e.g.) Rotor: S150AT

RCFmax: 850x1,000 xg

Press

0

, and press the

8

Enter button to enter the

setting.

 See Section 2-2-1 “Setting run conditions”.

RCFmax column

5

2-45

2-3-4 Displaying and seting RCF

Process Touch panel operation Screen display and notice

4

Press the button to

turn the RCFmax (RCFavg)
column to the SPEED column.

SPEED

RCF

 The SPEED value

of the rotor

displayed in

the ROTOR

indicator is

displayed on

the setting value

display field of

the SPEED

column.

This is the SPEED
value for the
RCF value
entered in step 3.

NOTE 　(1) To shift from the SPEED column to the RCF column, press the button.

SPEED

RCF

(2) This centrifuge is not capable of checking rotors. Select the correct rotor.

Especially when you wish to determine a speed based on an RCF value, selecting the wrong

rotor and thus setting an RCF value exceeding the capacity of your rotor will cause the system to

calculate and set a speed exceeding the maximum allowable speed. (However, this

ultracentrifuge has an overspeed detector, which prevents an overspeed run.)

(3) When you run this centrifuge at a speed determined based on an RCF value, there may occur a

slight error (of up to 2%) between the set RCF value and the actual value, because the speed

setting is set in increments of 1,000 rpm.

2-46

2-3-5 User login

After logging in, individual users can control the operation history of the centrifuge.

(1) Procedure for user login

Process Touch panel operation Screen display and notice

Press the USER column on the

1

Run screen.

2-3-5 User Login

USER column

Press the column of the

2

desired user name on the User
Management screen. Check
that the pressed column turns
blue and press the LOGIN
button.
(“Admin” was registered as a

user name for this centrifuge
before shipment.)

Enter the desired PIN on the

3

keypad display and press the

Enter button.

PIN is the number (4 digits)
which you entered on the PIN
registration screen (see
Section 2-4-6 (1)).

(“1111” is the PIN of “Admin”

registered as a user name for

this centrifuge before
shipment. If necessary,
change the PIN.)

 The User

Management
screen appears.

The pressed
column turns
from white to
blue.

LOGIN button

 The Login screen

appears.

PIN box

 The masked PIN

is displayed.

Enter button

2-47

2-3-5 User Login

Process Touch panel operation Screen display and notice

The user name is displayed on

4

the user column of the Run

●Run screen

returns to view.

screen.

USER column

NOTE 　(1) User login is required to start operation when the user lockout function has been enabled as

described in Section 2-4-6 (2) “User Lockout.” You cannot operate the centrifuge without logging

in. Perform registration according to Section 2-4-6 (1) “User Management” before attempting to

log in.

2-48

y

(

2-4 Features of the MENU screen

2-4 Features of the MENU screen

The Menu screen appears by pressing the MENU screen tab on the Touchscreen. These features are

designed to allow you to use the CS150NX with additional handy options (see figure 2-4-1).

MENU tab

Fig. 2-4-1 MENU screen

The functions of each icon on the MENU screen are described in the table below.

Function Icon Description

The information about the run parameters of a previous normal

Run History

Rotor Catalog

Spin-down

operation

RTC

(real-time control)

operation

Customize

operation can be stored automaticall
load it for a new run
loading the information about run parameters”).

You can view the applicable rotor names and their specifications (see
Section 2-4-2 “Rotor catalog”).

While you press the PULSE button on the Run screen and are
holding down it, the centrifuge accelerates up to the set speed at the
maximum acceleration rate. When you release this button, it starts
deceleration. This feature is useful to remove the adhered samples on
the interior walls of the tubes (see Section 2-4-3 “Spin-down operation”).

Run starts or completes at a required date and time (see Section 2-4-4
“RTC operation”).

You can select rotor stop signal etc. which enable you to easily run the
centrifuge (see Section 2-4-5 “Customizing the settings”).

see Section 2-4-1 “Displaying the Run History and

in the centrifuge and you can

Manager

(Admin)

Press the desired icon. Then the corresponding item is displayed.

You can set the items which the administrator should set such as
language (see Section 2-4-6 “Administrator (Admin) functions”).

2-49

2-4-1 Displaying the Run History and loading the information about run parameters

2-4-1 Displaying the Run History and loading the information about run parameters

The information about the run parameters of a previous normal operation can be

stored automatically in the centrifuge and you can load it for a new run.

Process

1

2

Touch panel operation Screen display and notice

Press the Run History
icon on the MENU screen.

If you want to load the
information about the run
parameters for a new run,
press the column of the
information which you want to
load for a new run. The column
turns from white to blue.
Check that the pressed column
turns blue and press the
Load for a new run button.

 The Run History screen

appears.

Select the column.

If you want to check the details
of the selected information,

press the View Details button.

3 Press the Run screen tab to

return to the Run screen.

 The Run History Details

screen appears.

The Run screen appears.

2-50

2-4-2 Rotor Catalog

You can view the applicable rotor names and their specifications.

2-4-2 Rotor Catalog

Process

1

Touch panel operation Screen display and notice

Press the Rotor Catalog
icon on the MENU screen.

< : Previous Page button

> : Next Page button

2 If you want to view the MENU

screen, press the x button or
MENU screen tab.

If you want to view the Run
screen, press the Run screen
tab.

 The Rotor Catalog screen

appears.

Number of tubes x ml

 The MENU screen appears.

 The Run screen appears.

2-51

f

2-4-3 Spin-down operation

2-4-3 Spin-down operation

While you press this button and are holding down it, the centrifuge accelerates up to the
set speed at the maximum acceleration rate. When you release this button, it starts
deceleration. This feature is useful to remove the adhered samples on the interior walls o
the tubes.

Process Touch panel operation Screen display and notice

1 Press the PULSE Operation

icon on the MENU screen.

● The PULSE button

is displayed on the

Run screen.

2 While you press the

PULSE button and are
holding down it, the rotor

● When the rotor stops, the PULSE button disappears and the

START button and the STOP button appears.

● To cancel the spin-down operation, press the tab of the MENU

rotates.

screen tab and press the PULSE Operation icon again.

NOTE 　(1) While performing the spin-down operation, you cannot change run parameters until the rotor

stops.

2-52

2-4-4 RTC (real-time control) operation

2-4-4 RTC (real-time control) operation

The CS150NX contains an internal clock, allowing you to run the machine at a

specified start or finish time for centrifugation. This feature for running the machine
at a specified time is called the RTC (real-time control) feature. The feature saves
you the trouble of calculating the delay time for “delayed-start operation”.

Explained below is how to perform an RTC operation, with an example.

Example:If you wish to install your rotor on the centrifuge under the run conditions listed below on the
evening of January 10 and to take out the samples around 8:00 the next morning;

(1) Rotor: S150AT
(2) RPM: 150,000 rpm
(3) Separation time: 2 hours
(4) Control temperature: 4 ℃
(5) Acceleration mode: 9
(6) Deceleration mode: 7

150,000

4:30

2h

January 10 January 11

6:00 8:04

8:00 am

Fig. 2-4-4 A typical RTC operation

In this example, you set the above run conditions (2) through (6), set the start time for RTC operation to 6:00,
January 11 and start the centrifuge.
(You can make an identical setting by setting the finish time to 8:00 instead of setting the start time to 6:00.)

2-53

2-4-4 RTC (real-time control) operation



t

(1) How to perform RTC an operation

Process

1

Touch panel operation Screen display and notice

Enter the run parameters on
the Run screen.

Press the RTC button.

To specify the run time (centrifugation time), do not set

If you want to set a run to start

HOLD. Enter the desired numeric value.
on a particular date and time,
press the Start Time button.

If you want to set a run to stop

Start time button

Stop time button

on a particular date and time,
press the Stop Time button.

Enter the start time or stop time
on the keypad display.

RTC　 button

 The Set RTC screen

appears.

 You can enter the star

time or stop time on
the keypad display.

 If you want to change any of the Month, Day, Hour, or Minuites

setting, press the column of the desired item and enter the
numeric value.

 To set the hours, use a number between 0 and 23 (in the 24-

hours system).

 Enter a time later than the current time. When setting the “stop

time”, allow for centrifugation time and set the centrifugation
start time to a time later than the current time.

 You cannot set the centrifugation start time to a date more than

20 days in the future.

2-54

s

2-4-4 RTC (real-time control) operation

t

Proces

Touch panel operation Screen display and notice

2 Press the Enter button.

Press the OK button and

3

the START button.

The RTC run does not ge

activated unless you press

the START button.

● The START

confirmation box

appears.

● The START time and STOP time for centrifugation are

displayed on the RTC field by pressing the START
button. This centrifuge will turn the rotor automatically at
the specified time.

● Pressing the START button enters the system into a

condition causing the centrifuge to wait until the set time
comes. This centrifuge will turn the rotor automatically at
the specified time and run it for the specified time.

● Once the RTC setting is made, you cannot change the

run time (centrifugation time). If you wish to make a
change to the run time, cancel RTC first. If you want to
cancel the RTC, press the RTC button on the Run
screen and press the Cancel button on the SET
RTC screen.

● If you cancel RTC when the user lockout function (see

Section 2-4-6 (2) on this function) is enabled, the following
screen appears. Inputting the “login user PIN” or the
“administrator PIN” is required in this screen.

2-55

2-4-4 RTC (real-time control) operation

NOTE

1. You cannot make an RTC setting in any of the following cases:
(1) When the Run screen is set to HOLD (continuous run)

Set the run time (centrifugation time) not to HOLD but to a numerical value.

(2) When it is past the start time

Set the start time to a time later than the current time.

(3) When the start time is more than 20 days after the current time

Set the time to a time no more than 20 days afterwards.

2. To change the run time (centrifugation time) after making an RTC setting, cancel RTC and then set a new

run time.

3.To perform a combination of a programmed operation (including a step-mode operation) with an RTC run,

call a program memory unit, then set RTC.
The system calculates the total run times of all steps of the programmed operation and calculates the start
time for RTC.
Therefore, cannot call the program memory unit after setting RTC.

4. To stop this ultracentrifuge in RTC operation, press the STOP button. The system then stops RTC and

stops the rotor.

2-56

2-4-5 Customizing the settings

f

2-4-5 Customizing the settings

You can customize the settings on the Run screen and the settings on the CUSTOM screen
such as the stop signal, sound volume and backlight.

Press the Customize icon on the MENU screen. Then the CUSTOM screen showing the five functions is
displayed as shown in Fig. 2-4-5.

Tab of each screen

Fig. 2-4-5 CUSTOM screen

The functions of each icon on the CUSTOM screen are described in the table below.

Function Icon Description

Zoom

Stop signal

Volume

Brightness

Vacuum
Standby

Setting

Press the desired icon. Then the corresponding item is displayed. After setting, press the tab o
the desired screen.

The display on the Run screen can be zoomed in (see
Section 2-4-5 (1)).

The rotor stop signal can be selected from six kinds of sound
including five tunes and electric beep (see Section 2-4-5 (2) ).

The volume of the stop signal can be adjusted (see Section
2-4-5 (3)).

The brightness of the screen can be adjusted (see Section 2­4-5 (4)).

The conditions of the waiting for the vacuum of the rotor
acceleration state can be specified (see Section 2-4-5 (5)).

2-57

x

c

2-4-5 Customizing the settings

(1) Zoom

The display on the Run screen can be zoomed in.

1. Normal:Displays the ordinary Run screen

2. Zoom:The speed and time display is zoomed when
20 seconds have passed after reaching the
set speed.

Fig. 2-4-5 (1) Zoom Setting screen

Press either the NORMAL or ZOOM button and make
sure that the selected button is surrounded with a green frame.
Then press the x button or the CUSTOM tab to store the setting.

To return from the zoom screen to the normal screen during operation, press anywhere except the

STOP button. Then the screen returns to the normal screen. Press the Customize icon of the MENU

screen and select the NORMAL according to the above procedure.

(2) Stop signal

The rotor stop signal can be selected from si
kinds of sound including five tunes and electri
beep.

Press the column of the desired stop signal. Then the
stop signal sounds and the column of the selected
stop signal turns blue.

Press the button to store the setting.

Enter
To set another function on the CUSTOM screen,
press the x button or the CUSTOM tab.

(3) Volume Adjustment

The volume of the stop signal can be adjusted.

The sound volume is turned up as the green range in
the volume setting indicator increases. The stop signal
does not sound if there is only the black range. Adjust
the sound volume by pressing the following buttons.

Fig. 2-4-5 (2) Stop signal Setting screen

< The volume is lowered by pressing this button.
　　 >　The volume is turned up by pressing this button.

The stop signal does not sound by pressing this button.

　　　　The stop signal sounds at full volume by pressing this button.

Press the x button or the CUSTOM screen tab to store the setting.

2-58

Fig. 2-4-5 (3) Volume Setting screen

(4) Backlight setting

The backlight brightens up as the green range in the
backlight setting indicator increases. The backlight
level is darkest if there is only the black range.
Press the following buttons to adjust the brightness of
the screen. Press the x button or the CUSTOM
screen tab to store the setting.

The brightness is decreased by pressing

<

>

The brightness of the screen can be adjusted.

this button.

The brightness is increased by pressing

this button.

2-4-5 Customizing the settings

Fig. 2-4-5 (4) Backlight Setting screen

(5) Vacuum standby setting

The conditions of the waiting for the vacuum of the
rotor acceleration state can be specified.

1. Normal:
When the vacuum level returns from medium to low
during acceleration at 5,000 rpm or higher speed, the
centrifuge keeps on running at that speed until the
vacuum level becomes medium again.

2. No Waiting Acceleration:
The centrifuge keeps on accelerating when it is

accelerating at 5, 000 rpm or higher speed even after
the vacuum level returns from medium to low.

Fig. 2-4-5 (5) Vacuum Standby Setting screen

Press either the Normal or No Waiting Acceleration button and make sure that the selected
button is surrounded with a green frame.
Then press the x button or the CUSTOM screen tab to store the setting.

NOTE (1) Select Normal mode when using the sample that is sensitive to a temperature rise. If you select

No Waiting Acceleration , the rotor does not wait until the vacuum reaches an intermediate level
and it might cause the rise in the rotor retention temperature by the friction heat.

(2) If the rotor chamber has frost on it by the repeated operation, it takes a long time to reach an

intermediate vacuum. In that case, wipe it off with a cloth, a sponge, etc.

(3) Once you select the condition of the waiting for the vacuum ( Normal or

No Waiting Acceleration ), your centrifuge memorizes this condition of the waiting for the

vacuum. If necessary, change the condition of the waiting for the vacuum.

(4)If the low vacuum condition persists for more than one minute in the No Waiting Acceleration

mode while the rotor rotates at the set speed, the alarm message “VACUUM ERROR” is
displayed and the rotor is stopped.

2-59

2-4-6 Administrator (Admin) function

2-4-6 Administrator (Admin) functions

You can set the admistrative items such as language mainly.

Press the Manager(Admin) icon on the MENU screen. Then the ADMIN screen showing the eight
functions is displayed as shown in Fig. 2-4-6.

Tab of each screen

Fig. 2-4-6 ADMIN screen

2-60

2-4-6 Administrator (Admin) function

The functions of each icon on the ADMIN screen are described in the table below.

Function Icon Description

User Management

User Lockout

Rotor Lockout

Japanese

Rotor Management

The user name can be registered and deleted
(see Section 2-4-6 (1)).

User login can be required to start operation
(see Section 2-4-6 (2)).

Run history of each rotor can be controlled
(see Section 2-4-6 (3)).

English and Japanese can be toggled in display
(see Section 2-4-6 (4)).

You can use and control the rotor data such as total operation
hours and number of runs (see Section 2-4-6 (5)).

Actual Run Timer

Date and time display

Centrifuge ID

Service contact

Actual run timer can be selected
(see Section 2-4-6 (6)).

The date and the time can be set
(see Section 2-4-6 (7)).

Centrifuge ID can be set for identification
(see Section 2-4-6 (8)).

The operation history data of the centrifuge can be exported to a

USB/LAN

communication

USB flash drive.
If your centrifuge has a LAN communication option,
LAN communication start/stoppage can be specified.

(see Section 2-4-6 (9)).

Press the desired icon. Then the corresponding item is displayed. After setting, press the tab of the desired
screen.

2-61

s

2-4-6 Administrator (Admin) function

(1) User Management

You can register up to 40 users with the system.
How to store (register) and how to change a user name are described below.

Proces

1

Touch panel operation Screen display and notice

Press the User Management
icon. Then press the
Add User button.

(“Admin” whose PIN is “1111”

has been registered in this
centrifuge before

shipment. If necessary,

change the PIN.)

Add User button

User name box

● The User

Management

appears.

x button

● A screen to

register a user

name is

displayed.

Clear button

Shift button

Space button

Delete button

Enter button

● Press the Shift button to toggle case.

● Press the Clear button to delete all the characters in

the user name box.

● Press the Space button to put one space after the last

character in the user name box.

● Press the Delete button to delete the last character in

the user name box.

● Press the Enter button to turn the screen to the PIN

registration screen.

● Press the x　button to return to the user administration

screen.

2-62

s

2-4-6 Administrator (Admin) function

Proces

2

Touch panel operation Screen display and notice

Register the user name and
touch the Enter button.

Enter button

PIN box

●The PIN registration

screen is displayed.

3

Enter the desired PIN number
(4 digits) in the PIN box and
touch the Enter button.

●The masked PIN

number is displayed.

●The PIN confirmation

screen is displayed.

2-63

s

A

2-4-6 Administrator (Admin) function

Proces

4

Touch panel operation Screen display and notice

Enter the same PIN number (4
digits) as the one entered in the

PIN (confirm) box

above step 3 in the PIN
(confirm) box. Press the Enter
button.

Difference of access level
Admin: The administrator can

do every operation of
this centrifuge.

Enter button

User: The user cannot use the

Admin functions when
the user lockout function
is enabled.

● The masked PIN

number is displayed.

● The access level

selection box is

displayed.

ccess level

selection box

To place the registered user on

5

the same access level with the
administrator, press
Administrator in the access
level selection box. If you do
not want to place the registered
user on the same access level
with the administrator, press
User ..

(The screen display shows the

case when placing the
registered user on the same
access level with the
administrator.)

For the difference of access
level, refer to Section 2-4-6 (2).

● The screen returns

to the User
Management
screen.
”Hitachi-Koki” is
added to the user
name and
“Administrator” is
displayed as the

Access level button

PIN button

● To change the access level or the PIN number after

access level.

registration of the user name, press the column of the
desired user name and check that the column turns blue.
Press the Access level button or the PIN button to
make a change.

● To delete the user name after registration, press the

column of the desired user name and check that the
column turns blue. Press the Delete User button to
delete it.

2-64

（2）User Lockout

r

Enable : User login is required to start operation.

You can not set run conditions and start

operation without logging in. Prior user

registration is required.

(See Section 2-4-6 (1) “User control” and 2-3-5 “User login.”)

In addition, login of a user having the administrator access level

2-4-6 Administrator (Admin) function

is required to call up the Admin screen.)

Fig. 2-4-6 (1)　User Lockout Setting screen

Disable : User login is not required to start operation.

Press either the Enable or Disable button and make sure that the selected button is surrounded
with a red frame.
Then press the x button or the ADMIN screen tab to store the setting.

（3） Rotor Lockout

Enable : Select a rotor from the registered rotors

to start operation. It is impossible to

select from the rotor catalog. (See

Section 2-4-6 (5) “Rotor Management.”)

Disable : The centrifuge is operable without selecting the

rotor. It is possible to select a rotor from the roto

catalog.

Fig. 2-4-6 (2)　Rotor Lockout Setting screen

Press either the Enable or Disable button and make sure that the selected button is surrounded
with a red frame.

Then press the x button or the ADMIN screen tab to store the setting.

（4）Japanese

You can select whether to give displays in English or Japanese by pressing this button.

2-65

2-4-6 Administrator (Admin) function

f

（5） Rotor Management

You can use and control the rotor data such as total operation hours and number o
runs by registering your rotors in the centrifuge in advance. (It is recommended to use
the rotor lockout function (see 2-4-6 (3)) together in order to control the rotor data such
as total operation hours and number of runs.)

(1) Registering the rotor

Process Touch panel operation Screen display and notice

1 Procedures for registering the

rotor are described as follows.

a） Press the

Rotor Management
icon on the ADMIN screen.

b） Press the Add rotor

button.

a） The Rotor

Management
screen
appears.

b）The ROTOR

CATALOG

screen appears.

c) Press the column of the

desired rotor name and

press the Enter

button.

d) Enter the S/N (Serial

number ) and press the

Enter button.

c ） The Enter S/N

screen appears.

● Enter a 4-digit

number: In the

case of “123,”

prefix zero to the

number (“0123”).

d) Registration is

completed if it is

a new rotor

having no

operation history.

2-66

2-4-6 Administrator (Admin) function

Process Touch panel operation Screen display and notice

2 Procedures for entering the

total operation hours of the
rotor are described as follows.

a） Press the column of the

desired rotor on the Rotor
Management screen.
Then check that the
pressed column turns blue

and press the Edit Hours
button.

b） Enter the total operation

hours of the rotor and press
the Enter button.

● The total operation

hours of a rotor

having operation

history can be

registered. This

operation is not

required for a new

rotor.

2-67

2-4-6 Administrator (Admin) function

Process Touch panel operation Screen display and notice

3 Procedures for entering the

total number of runs of the rotor
are described as follows.

a） Press the column of the

desired rotor on the Rotor

Management screen.
Then check that the
pressed column turns blue

and press the Edit Runs
button.

b） Enter the total number of

runs of the rotor and
press the Enter
button.

● The total number

of runs of a rotor

having operation

history can be

registered. This

operation is not

required for a new

rotor.

4 Press the Run screen tab. ● The Run screen appears.

2-68

r

2-4-6 Administrator (Admin) function

(2) Deleting a registered rotor

Process Touch panel operation Screen display and notice

1 Press the Rotor Management

icon on the ADMIN screen.

2 Press the column of the

rotor which you want to delete
on the Rotor Management
screen.
Then check that the pressed
column turns blue and press

the Delete rotor button.

● The Rotor

Management

screen appears.

● The deletion

confirmation box

is displayed.

3 Press the Yes button on

the deletion confirmation box.

●The registerd roto

is deleted.

2-69

t

r

2-4-6 Administrator (Admin) function

（6） Actual Run Timer setting

<Actual Run Timer is enabled.>

When you press the Enable button,

the timer starts counting after reaching the

set speed.
<Actual Run Timer is not enabled.>

When you press the Disable button,

Fig. 2-4-6 (3)　Actual Run Timer Setting screen

the timer starts counting immediately afte

the start of operation.

Press either the Enable or Disable button and make sure that the selected button is surrounded
with a red frame.

Then press the x button or the ADMIN screen tab to store the setting.

（7） Date and time

Use this function to precisely set the curren
time setting of the internal clock.
Set an exact date and time for RTC
operation.

Fig. 2-4-6 (4)　Setting at time screen

Press the column of the desired item (Year, Month, Day, Hour, or Minute). Check that the pressed

column turns to red and enter the date and time on the keypad display.

If you want to change the other items, press the column of the other items.

You can also turn the desired column to red by pressing the > button and < button.
If you do not want to change the other items, press the　Enter button on the keypad display.

Example:
Year:2008
Month:December
Day:11th
Time:8:58am

Fig. 2-4-6 (5)　Setting at time screen

If you want to change the setting of the other items on the ADMIN screen, press the ADMIN screen tab.

2-70

(8) ID /Contact

Press the Machine ID column.

2-4-6 Administrator (Admin) function

When you have two or more centrifuges, you can set the centrifuge ID for each
centrifuge for identification.

Service contact:

Set the centrifuge ID
(4-digit number).

Make a memo of your
service person’s contact
address.

Fig. 2-4-6 (6) Centrifuge ID and service contact setting screen

Press the Service contact column.

Fig. 2-4-6 (8) Enter Service contact screen Fig. 2-4-6 (7) Enter Centrifuge ID screen

（9） USB/LAN communication

[USB MEMORY]

The operation history of the centrifuge can be output in CSV format* to a USB flash drive on the market.
*CSV format: This is a versatile file format that is compatible with spreadsheet softwares such as EXCEL.

USB (host side)

Insert the USB flash drive on
the market into the USB for
external connection (host
side) at the right side of the

Press the DATA EXPORT
button.
(Writing in the USB flash drive

is automatically started.)

centrifuge.

Fig. 2-4-6 (9) USB/LAN communication setting screen

2-71

2-4-6 Administrator (Admin) function

NOTE (1) Be After writing in the USB flash drive is finished, the following screen appears.

If you press the YES button, the run history data stored in the centrifuge is deleted.
If you press the NO button, the run history data stored in the centrifuge is not deleted.
However, if the information about 100 sets of the run parameters of the previous normal
operations has been stored in the centrifuge, the oldest information of the run parameters is
deleted when you perform the centrifuge run.

(2) Be sure to back up your data before using the USB flash drive. Hitachi Koki will not be liable for

any data loss.

(3) This centrifuge cannot use the USB flash drive with security function.
(4) When powered on with the USB flash drive, the centrifuge may not recognize the USB flash

drive. In such a case, pull out the USB flash drive from the centrifuge and then insert it again
into the centrifuge.

[LAN Communication]

If your centrifuge has a LAN communication option,
LAN communication start/stoppage can be specified.
Insert the LAN cable into the LAN port at the right side of the centrifuge.

The following message is displayed.
“Transmission was completed. Do

you want to delete run history data
stored in centrifuge?”

LAN port（option）

NOTE

Connect :

Disconnect : LAN communication stops.

Even if your centrifuge does not have a LAN communication option, the Connect button is
surrounded with a red frame as you press the Connect button. In that case, this icon is
always displayed on the Run screen, but there is no problem.

LAN communication starts.
Press this button to establish the communication between “himac LogManager” (option)
on your computer and your centrifuge. It is possible to manage operation results of this
centrifuge by using “himac LogManager” (option). If you press this button, either of the
below icons is displayed on the upper side of the VACUUM button on the Run screen.

：There is no problem in communication between “himac LogManager” (option) on

your computer and your centrifuge.

：There might be problems in communication between “himac LogManager” (option)

on your computer and your centrifuge. Follow the below instructions.

 Check whether the LAN cable is connected to the centrifuge properly.
 Check whether the “himac LogManager” on your computer is started.
 Check whether the LAN works properly.

2-72

2-5 Emergency recovery from power failure

2-5 Emergency recovery from power failure

DANGER: Before removing the front cover for troubleshooting, always turn off the POWER switch of

the instrument, unplug the power cord from the wall outlet, and wait at least three minutes
to avoid the risk of electrical shock.

WARNING:

CAUTION:

1. Rotation of the rotor

The rotor stops as a result of free coast. However, if the rotor did not decelerate to 300 rpm or lower when

a power failure occurred, it will, or lower when a power failure occurred, after power returns, automatically

accelerate again and return to the set speed before the power failure. If the speed goes below 300 rpm, the

rotor will decelerate and stop after power returns.

If the rotor is stopping when the power returns, this centrifuge does not accept the VACUUM button etc.
(see Section 4-1 “Alarm indicators POWER FAILURE : ***** rpm”).

2. Touchscreen display

All displays are off during a power failure. After power returns, the instrument starts control again at the

settings specified before the power failure. (The settings have battery-backed back-ups.)

The instrument then displays an alarm message, indicating that it has suffered a power failure.

3. Removing the rotor from the ultracentrifuge

If a power failure may last a long time, open the door and take out the rotor as described below.

(1) Never open the door during rotation.

(2) Never touch the rotor during rotation.

Never conduct operations in a manner other than as described in this operation manual.

Contact with your nearest service representative if any problem arises.

WARNING:

The rotor chamber is in a vacuum with low air resistance. The rotor may therefore go on turning for

approximately 120 minutes before stopping. Allow enough time before opening the door.

Before following this procedure, listen carefully for sound from within the rotor chamber

and make sure that the rotor is not turning.

While the rotor is rotating, never unlock the chamber door.

2-73

2-5 Emergency recovery from power failure

(1) Make sure that the rotor has stopped.

(2) Unplug the power cord of the centrifuge from the wall outlet.
(3) Remove the four screws that fasten the both sides of the front cover by Hex key, remove the front

cover while pulling it towards you, and remove the cover.

Top deck

Vacuum

Front cover

release knob

Front cover fastening screw

Front cover hook

Fig. 2-5-1. Removing the front cover

(4) Remove the vacuum release knob at the left of the vacuum chamber (by turning its tip

counterclockwise) to allow air to enter the rotor chamber. When the rotor chamber reaches

atmospheric pressure, do not forget to return the vacuum release knob to its previous position.

2-74

2-5 Emergency recovery from power failure

r

(5) Pull the release wire of the door lock in the left side of the vacuum chamber toward you, and open the door.

After opening the door, make sure that the rotor is not rotating. If it is rotating, close the door immediately.

WARNING: Never touch the rotor while it is rotating.

Vacuum chambe

Vacuum release knob

Release wire of the door lock

Square hole of the frame

Fig. 2-5-2 Door lock and vacuum release knob

(6) After taking out the rotor, insert the hooks (which are on both sides of the front cover) into the frame, and

insert the front cover into the gap between the top deck and the front cover supporting plate while holding

up the front cover a little. Then fasten them with the fastening screws.

Top deck

Front cover

Front cover supporting plate

Hook

Fig. 2-5-3 Inserting the front cover

(7) Plug the power cord into the wall outlet.

(8) After the power returns, turn on the power switch of the centrifuge. Press the VACUUM button to turn

on the vacuum pump for approximately 15 minutes, and then press the VACUUM button to turn off the

vacuum pump. Then turn off the power switch on the centrifuge. (These procedures are effective ways of

keeping the vacuum pump in good condition.)

2-75

3. Maintenance

3. Maintenance

Be sure to read and keep in mind the following cautionary information before maintenance.

DANGER: Before removing the cover, top deck, or other component for maintenance, always turn

off the POWER switch of the instrument, unplug the power cord from the wall outlet,
and wait at least three minutes to avoid the risk of electrical shock.

WARNING: (1) If the centrifuge, rotor, or an accessory is contaminated by samples that are toxic

or radioactive, or blood samples that are pathogenic or infectious, be sure to
decontaminate the item according to good laboratory procedures and methods.

(2) If there is a possibility that the centrifuge, rotor, or an accessory is contaminated

by samples that might impair human health (for example, samples that are toxic or
radioactive, or blood samples that are pathogenic or infectious), it is your
responsibility to sterilize or decontaminate the centrifuge, rotor, or the accessory
properly before requesting repairs from a Hitachi Koki authorized sales/service
representative. Note that Hitachi Koki cannot repair the centrifuge, rotor, or the
accessory unless sterilization or decontamination is completed

.

(3) It is your responsibility to sterilize and/or decontaminate the centrifuge, rotor, or

parts properly before returning them to a Hitachi Koki authorized sales/service
representative. In such cases, copy the decontamination sheet at the end of this
manual and fill out the copied sheet, then attach it to the item to be returned.
Hitachi Koki may ask you about the treatment for the centrifuge, rotor or the part if
the decontamination is checked and judged as insufficient by Hitachi Koki. It is
your responsibility to bear the cost of sterilization or decontamination.

If you have any question, please send e-mail to[e-mail].

Note that Hitachi Koki cannot repair or inspect the centrifuge, the rotor or the
accessory unless sterilization or decontamination is completed.

CAUTION: Do not perform any operation not specified in this manual. If any problem is found on

your centrifuge, contact a Hitachi Koki authorized sales/service representative.

3-1

3-1 Rotor chamber
3-2 Drive shaft (Crown)
3-3 Cabinet

This centrifuge does not require complicated maintenance and inspection. For longer and safe use of
this centrifuge without trouble, observe the following instructions.

CAUTION: Using a cleaning or sterilization method other than the ones recommended in this

instruction manual might cause corrosion or deterioration of the centrifuge. Refer to
the chemical resistance chart provided with the rotor, or contact Hitachi Koki.

CAUTION: Disconnect the power cord from the outlet before cleaning or sterilizing the centrifuge.

For information on the maintenance of rotors and tubes, see the rotor instruction manual provided with
the rotor.

3-1 Rotor chamber

CAUTION: Do not pour any solution such as water, detergent, or disinfectant directly into the

rotor chamber and be careful not to spill the sample into the rotor chamber. If you do
so, the bearings of the drive unit might corrode or deteriorate and it might cause
vacuum failure.

To maintain the rotor chamber, follow the instructions given below:
(1) When the ultracentrifuge is not in use, keep the rotor chamber ventilated.
(2) If the bowl is moist, wipe it with a clean, dry cloth or sponge.
(3) If the rotor chamber is dirty, wipe it with a clean cloth wrung out in a diluted solution of a mild, non-

alkaline detergent. For sterilization, wipe it with a cloth etc. dampened with 70% ethanol.

3-2 Drive shaft (Crown)

CAUTION: Clean the inside of the drive hole (crown hole) of the rotor and the surface of the drive

shaft (crown) of the centrifuge once a month. If the drive hole or the drive shaft is
stained or any foreign matter is adhered, the rotor may be improperly installed and
come off during operation

This part is very important because the rotor is mounted on it and the crown transmits driving force to
the rotor. Before mounting a rotor, wipe the outer surface of the crown with a soft cloth dampened with
water sufficiently.

3-3 Cabinet

Always keep the top deck and the cabinet of the centrifuge clean to prevent dust and other materials
from falling into the rotor chamber. Wipe the top deck and the cabinet with a cloth or sponge
dampened with a diluted solution of neutral detergent. If any solution that is toxic, radioactive, or
pathogenic is spilled inside or outside the centrifuge, take necessary action according to your proper
laboratory procedures and methods.

3-2

3-4 Chamber door seal

3-4 Chamber door seal

If the door seal O-ring is dusty or scratched, high vacuum level will not be obtainable. Always keep the
door seal O-ring clean. When the ultracentrifuge is used frequently, take out the door seal O-ring and
wipe it with a clean, soft cloth and then put a thin coat of vacuum grease on it every three to four months
(ordinarily, once a year). If the door seal O-ring is damaged, replace it. Wipe the groove for the door seal
O-ring with a clean, soft cloth dampened with alcohol or a similar solvent.

3-4-1 Removing the door seal O-ring

1. While the door is open, turn off the POWER switch and unplug the power cord from the wall outlet.

2. Open the door completely. Then hold the handle of the door and pull up the door.

Handle

Door seal O-ring

Fig. 3-4-1Removing the door seal O-ring

3. Use a toothpick etc. to take the door packing out of the groove. At that time, be careful not to damage
the door seal O-ring or groove.

4. Clean the door seal O-ring and groove. If the door seal O-ring is damaged, replace it. {Part code of
replacement door seal O-ring (not furnished with the accessories): 84520135}

5. Apply a thin coat of vacuum grease to the door seal O-ring, insert it into the groove, and close the

door.

3-3

3-5 Vacuum pump
3-6 Others

3-5 Vacuum pump

If displaying the VACUUM alarm, poor evacuation, or other trouble occurs frequently, the vacuum
pump oil may deteriorate or the oil mist trap may be clogged or the vacuum pump may have a
problem. The vacuum pump oil should be changed once a year to prevent deterioration of the
vacuum pump and clogging of the oil mist trap. Note that the oil change frequency may differ
depending on the use conditions and the environment. Call a service representative when an oil
change is required or any trouble occurs.

Oil for the vacuum pump is furnished with this centrifuge. Store it in a safe place and give it to the
service representative if requested.

3-6 Others

(1) Storage period of service parts

Service parts are kept in stock ten years after the discontinuation of production.
The term “service parts” means the parts that are necessary to ensure the correct functioning of the
centrifuge.

3-4

4. Troubleshooting

4. Troubleshooting

Be sure to read and keep in mind the following cautionary information before troubleshooting.

DANGER: Before removing the cover, top deck, or other component for troubleshooting, always

turn off the POWER switch of the instrument, unplug the power cord from the wall
outlet, and wait at least three minutes to avoid the risk of electrical shock.

WARNING: (1) If the centrifuge, rotor, or an accessory is contaminated by samples that are toxic

or radioactive, or blood samples that are pathogenic or infectious, be sure to
decontaminate the item according to good laboratory procedures and methods.

(2) If there is a possibility that the centrifuge, rotor, or an accessory is contaminated

by samples that might impair human health (for example, samples that are toxic or
radioactive, or blood samples that are pathogenic or infectious), it is your
responsibility to sterilize or decontaminate the centrifuge, rotor, or the accessory
properly before requesting repairs from a Hitachi Koki authorized sales/service
representative. Note that Hitachi Koki cannot repair the centrifuge, rotor, or the
accessory unless sterilization or decontamination is completed

.

(3) It is your responsibility to sterilize and/or decontaminate the centrifuge, rotor, or

parts properly before returning them to a Hitachi Koki authorized sales/service
representative. In such cases, copy the decontamination sheet at the end of this
manual and fill out the copied sheet, then attach it to the item to be returned.
Hitachi Koki may ask you about the treatment for the centrifuge, rotor or the part if
the decontamination is checked and judged as insufficient by Hitachi Koki. It is
your responsibility to bear the cost of sterilization or decontamination.

If you have any question, please send e-mail to[e-mail].

Note that Hitachi Koki cannot repair or inspect the centrifuge, the rotor or the
accessory unless sterilization or decontamination is completed.

CAUTION: Do not perform any operation not specified in this manual. If any problem is found on

your centrifuge, contact a Hitachi Koki authorized sales/service representative.

Before removing the cover, top deck, or other component for troubleshooting, always turn off the
POWER switch of the instrument, unplug the power cord from the wall outlet, and wait at least three
minutes to avoid the risk of electrical shock.

4-1

4-1 Alarm indicators









This ultracentrifuge incorporates a “self-diagnosis feature” that diagnoses the cause of any problem
which may occur when you start the centrifuge or while in operation.

4-1 Alarm indicators

If any trouble occurs, this machine gives a buzzer sound and displays an alarm message in the message
field of the Run screen to warn of the trouble.

(1) Responding to an alarm signal

If an alarm message appears, remove the cause of the trouble as described below and press the
CE button. You will then be able to resume your centrifugation.

WARNING:

Unspecified repairs, remodeling or disassembly of the centrifuge that is not listed

below is strictly prohibited by any person other than a Hitachi Koki authorized services
representative.

If the alarm message persists even after you have done what is specified below, contact service
representative to order a repair.

Alarm Cause Action

CLOSE DOOR

Close the door completely.

Press the VACUUM or START
button.

VACUUM ERROR

Wipe off moisture inside the rotor
chamber, or check a sample leakage.

1. The VACUUM or START button has
been pressed with the chamber door
left open.

1. Required level of vacuum cannot be
reached.

2. After a satisfactorily high level of

vacuum was reached, it lowered (due
to, for instance, sample leakage).

 Close the door completely and press the

VACUUM or START button.

Wipe off the moisture from inside the rotor

chamber.

 Clean the door packing, then apply a thin coat

of vacuum grease. (Refer to Section 3-4
“Chamber door seal.”)

 Check if the sample is leaking from the rotor

and/or tubes. If so, the tubes may be overfilled
or may be cracked or broken. In these cases,
reduce the amount of sample in the tubes or
change the tubes.

SET ROTOR

Set the rotor. Wait up to 20 min.

If the alarm is not cleared.

IMBALANCE

Check balance of samples.

Tighten rotor cover or cap securely.

1. The rotor is not installed.

1. Rotor is not properly balanced, and
abnormal vibration has occurred in
the rotor.

2. Rotor cover or cap is not properly
tightened.

 Install the rotor.

 If a “SET ROTOR” alarm message appears,

this ultracentrifuge does not accept the CE or
VACUUM button for 20 minutes to ensure
safety. Wait at least 20 minutes, then press　the
CE button.

If you want to clear this alarm when 20 minutes
have not passed since this alarm message
appeared, see the ACTION of the alarm
message “POWER FAILURE：***** rpm”.

 Check if the sample tubes exceed allowable

imbalance level.

Check if any one of the tubes is deformed, and

if there is any sign of sample leakage.

 Tighten rotor cover or cap securely.

INVALID SPEED

Check max. speed of the rotor.

Set its max. or lower speed again.

POWER FAILURE

Running re-started automatically or
is suspended. Check status.

1. Rotor speed is set higher than the
maximum allowable speed.

1. A power failure occurred while the
rotor was rotating.

Refer also to Section 2-5 “Emergency recovery from power failure.”

 Set the speed within their permitted limits.

Unless the set run time has elapsed, restart

the run.

 If the instrument was automatically restored

and the rotor is rotating at set speed, then let
the run continue.

4-2

4-2 User-corrected Problem

Alarm Cause Action

POWER FAILURE：***** rpm

Door is closed. Refer to manual for
further detail.

E11-E64

1. A power failure occurred while the rotor
was rotating and the speed could not be
detected after power returned.

(This alarm code appears when the

detector of this ultracentrifuge could not
identify which status (status 1 or status
occurred.

Status 1: The rotor stopped normally.
Status

2: Although the rotor continued to

rotate, the speed of the rotor
was not detected due to a

If this alarm code appears, this ultracentrifuge
does not accept the CE or VACUUM button
for 120 minutes to ensure safety (The same
thing can be said of “E13”).

Refer also to Section 2-5 “Emergency recovery from power failure.”

Refer to NOTE.

failure of the speed detector.

 This ultracentrifuge does not accept the CE or

VACUUM button for 120 minutes from power
failure.
If you are convinced that the rotor has come to a
complete stop, press the SPEED column,

2)

HOLD , 9 , 0 , 1 , HOLD and
Enter button in order when the Run
screen is displayed. Then press the VACUUM
button. This ultracentrifuge ac cepts the
VACUUM button.
First open the door a little to confirm that rotor
has come to a complete stop.
Then open the door properly.

 Call a service representative.

If any alarm message between E11 and E64 appears, this ultracentrifuge requires maintenance by a
service representative. When ordering a repair, inform us of the alarm code you have received.

E13 is an alarm code that indicates a failure of the speed detector.

NOTE

If this alarm code appears, this machine does not accept the CE or VACUUM button
for 120 minutes to ensure safety.
These 120 minutes are required for the rotor to stop.
Wait at least 120 minutes before pressing the CE button.
(This “120 minutes” is the time which passed after a power failure occurs.)

4-3

4-2 User-corrected Problem





4-2 User-corrected Problem

Some problems are not identified and reported by the self-diagnostic capability of the ultracentrifuge.
To correct these problems, take the action(s) described in the table below.

Symptom Possible cause Action

Ultracentrifuge will not start up
when its power is on.

Rotor does not cool,

or rotor temperature is rising.

You press the START button,
but the rotor will not turn.

The backlight level of the screen
is too dark or too bright.

You can not open the door
because the STOP button
continues to blink.

1. Building power circuit breaker is open.

2.The power cord is unplugged from the
outlet.

1. Rotor was accelerated at low or

intermediate vacuum level.

2. High vacuum level cannot be reached.

1. If the “SET ROTOR” alarm message
appears, the overspeed detector may
issue a signal that prevents the rotor from
rotating even though you pressed the CE
button. (This is not a fault.)

1. The brightness of the screen

adjusted.

1. You pressed the START button, and

then you pressed the STOP button

before the rotor started to rotate.

is not

 Close the circuit breaker.

 Plug the cord into the outlet.

 Start accelerating the rotor when the rotor

chamber is at high vacuum level (after the
vacuum indicator is displayed as the
following).

In a high vacuum

 Inspect the door seal O-ring (see Section

3-4).

 Turn off the POWER switch, wait several

minutes, turn on the POWER switch
again, and press the START button.

If this procedure still does not activate this
ultracentrifuge, call a service representative.

 Make the adjustment while referring to the

clause “Backlight setting” in Section 2-4-5
“Customizing the settings”

Because the detector of this ultracentrifuge

could not identify which status (status 1 or
status 2) occurred, the safety devices
function.
The centrifuge maintains deceleration
status at that time.
Status 1: The rotor stops normally.
Status 2: The speed detector is faulty.
Open the door of the centrifuge after the
safety devices are released (after from 10
seconds to approximately four minutes).

4-4

5. Installation

5. Installation

This chapter describes the electrical power requirements, location and environment that you must
provide for your ultracentrifuge prior to its installation by an authorized Hitachi Koki representative.

DANGER: Before removing the cover or other component, always turn off the POWER switch of

the instrument, unplug the power cord from the wall outlet, and wait at least three
minutes to avoid the risk of electrical shock.

Note: The installation and leveling of your ultracentrifuge must be done by an authorized Hitachi Koki

representative. If they are done by anyone else, the ultracentrifuge warranty will be null and void.

1.Place of installation

(1) Position the centrifuge on a level surface, such a table or laboratory bench that can support the

weight of the centrifuge and resist vibration. (Place the centrifuge at least 5 cm away from the
edge of the laboratory bench.)

(2) Ambient temperature for operation is 5 to 35°C. If the room temperature rises above 35°C, the

temperature of the rotor may become too high. Avoid installing the ultracentrifuge in direct
sunlight.

(3) Keep the back of the instrument at least 8 cm away from the wall. We recommend you install the

sides of the instrument about 13cm away from the walls.
Make sure that the air can circulate adequately around this ultracentrifuge. Avoid installing this
ultracentrifuge close to a heat-generating device, which might reduce this centrifuge’s cooling
capacity.

WARNING: For operator safety, maintain a 30-cm “clearance envelope” around the instrument

and keep out that area while the rotor is spinning. Do not store dangerous
substances capable of developing flammable or explosive vapors on nor near the
centrifuge.

8cm or more

13cm

or more

13cm

or more

Fig.5-1 Clearance for ultracentrifuge

5-1