Hitachi L-2100 Instruction Manual

INSTRUCTION MANUAL

FOR

MODEL L-2100 PUMP



PREFACE

Thank you very much for purchasing Hitachi Model L-2100/2130 pump, which has been specifically designed for automatic analysis in a liquid chromatograph system. The Model L-2100/2130 pump is intended for use by persons having a basic knowledge of chemical analysis. Remember that improper use of analytical instruments, chemicals or samples would result not only in wrong analytical data but also in consequences adverse to safety. Carefully read this instruction manual before attempting operation. For proper use of the pump, please acquaint yourself with it.

ABOUT THIS MANUAL

The operating procedures and maintenance/checkup instructions for the L-2100/2130 pump are described in this manual. First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the beginning of this manual for ensuring safety in operation of the pump. If installation of the pump is not yet completed, refer to Section 2. Before attempting operation, read through Sections 3 and 4 to become acquainted with the structure of the instrument and its basic operations.

- 1 -

- 2 -

IMPORTANT

Precautions on Electromagnetic Wave Interference

1. Possible Electromagnetic Wave Interference Caused by This Instrument

Where this instrument is used in a residential area or an adjacent area thereto, it may cause interference to radio and television reception. To prevent this, use the specified system connection cables in strict accordance with the instruction manual. The instrument is designed to minimize possible electromagnetic wave interference caused by it if the specified cables are connected properly. However, there is no guarantee that electromagnetic wave interference will not be caused by the instrument. If the instrument does cause interference to radio or television reception, which can be determined by turning the instrument off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

Reorient the antenna of the radio/television receiver.

•

Increase separation between the instrument and the radio/TV

•

receiver.

Connect the instrument to an outlet on a circuit different from

•

that to which the radio/TV receiver is connected.

2. Possible Electromagnetic Wave Interference Affecting This Instrument

Where this instrument is used near an intense electromagnetic source, interference noise may be given to the instrument to incur an adverse effect on its performance or functionality. To present this, use the specified system connection cables in strict accordance with the instruction manual. The instrument is designed to minimize possible electromagnetic wave interference affecting it if the specified cables are connected properly. However, there is no guarantee that electromagnetic wave interference will not occur in the instrument. If the instrument does incur electromagnetic wave interference, which can be determined by turning on and off possible sources of electromagnetic interference nearby, the user is encouraged to try to correct the interference by one or more of the following measures:

- 3 -

Warranty on Product

Reorient the instrument.

•

Increase separation between the instrument and possible

•

sources of electromagnetic interference.

Connect the instrument to an outlet on a circuit different from

•

that to which possible sources of electromagnetic interference are connected.

Check that any other device connected with the instrument is

•

not affected by electromagnetic interference.

The Model L-2100 pump is warranted to be free from defects in material or workmanship under normal use within the product specifications indicated in this manual and under conditions given below. This warranty is void if the instrument is not used according to the instruction manual.

(1) Scope of Warranty

Any parts which prove to be defective in design or workmanship during the warranty period will be repaired, adjusted or replaced without charge. A substitute part may be used for repair, or replacement with an equivalent product may be made instead of repair. Such system components as a personal computer and printer to be updated frequently for improvement may not be available in original versions at the time of replacement. Note that this warranty does not apply to the instrument if modified by the user or resold without permission from the manufacturer, consumable parts, and any failure of lifetime-expired parts.

(2) Warranty Period

One year from the date of initial installation

(3) Availability of Technical Support Service

Technical support service for this instrument is available within regular working hours on workdays specified by Hitachi High-Technologies Corporation.

- 4 -

(4) Limitations and Exclusions on Warranty

Note that the following cases are excluded from the scope of this warranty.

(a) Failure due to operation at a place not meeting the

installation requirements specified by Hitachi HighTechnologies Corporation.

(b) Failure due to power supply voltage/frequency other

than specified by Hitachi High-Technologies Corporation or due to abnormality in power supply.

impurities contained in reagent, gas, air or cooling water supplied by the user.

(d) Corrosion of the electric circuits or deterioration of the

optical elements due to highly corrosive atmospheric gas.

(e) Failure due to use of hardware, software or spare parts

other than specified by Hitachi High-Technologies Corporation.

(f) Failure due to improper handling or maintenance by

the user.

(g) Failure due to maintenance or repair by a service

agent not approved or authorized by Hitachi HighTechnologies Corporation.

(h) After disposal of this instrument, or after its resale

without Hitachi High-Technologies Corporation approval.

(i) Failure due to relocation or transport after initial

installation.

(j) Failure due to disassembly, modification or relocation

not approved by Hitachi High-Technologies Corporation.

(k) Consumables, and failure of parts that have reached

the end of specified useful life.

(l) Failure of parts excluded from the warranty in the

instruction manual or other documents.

(m) Failure due to acts of God, including fire, earthquake,

storm, flood, lightning, social disturbance, riot, crime, insurrection, war (declared or undeclared), radioactive pollution, contamination with harmful substance, etc.

(n) Failure of the hardware, or damage to the system

software, application software, data or hard disk due to computer virus infection.

- 5 -

(o) Failure of the personal computer connected with the

instrument, or damage to the system software, application software, data or hard disk due to power interruption or momentary power voltage drop caused by lightning.

(p) Failure of the personal computer connected with the

instrument, or damage to the system software, application software, data or hard disk due to disconnection of main power to the personal computer without taking the specified normal shutdown procedure.

(5) Disclaimer of Warranty

THE MANUFACTURER MAKES NO WARRANTIES, EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION THEREOF, WARRANTIES AS TO MARKETABILITY, MERCHANTABILITY, FOR A PARTICULAR PURPOSE OR USE, OR AGAINST INFRINGEMENT OF ANY PATENT. IN NO EVENT SHALL THE MANUFACTURER BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT. NO ORAL OR WRITTEN INFORMATION OR ADVICE GIVEN BY THE MANUFACTURER, ITS DEALERS, DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL CREATE A WARRANTY OR IN ANY WAY INCREASE THE SCOPE OF THIS WARRANTY.

(6) Warranty Card

The warranty card will not be reissued. Keep it in a safe place.

Installation, Relocation and After-sale Technical Service

Installation of the instrument shall be carried out by or under supervision of qualified service personnel of the manufacturer or its authorized service agent. Before installation of the instrument, the customer is requested to prepare necessary utilities referring to this manual. When relocation of the instrument becomes necessary after initial installation (delivery), please notify your local Hitachi HighTechnologies sales representative or service office.

- 6 -

Technical Seminars and Training for Customers

For the customers to acquire in-depth understanding of the analytical instruments, technical seminars and customer training courses are available at Hitachi High-Technologies Corporation or your site. For further information, contact your local Hitachi High-Technologies Corporation sales representative. (The technical seminars and customer training courses are available on a chargeable basis.)

Other Precautions

1. Handling of Chemicals and Samples

(1) The user is responsible for following relevant legal

standards and regulations in the handling, storage and discarding of chemicals and samples used in analytical operations of the instrument.

(2) Reagents, standard solutions and accuracy-control

samples shall be handled, stored and discarded as instructed by the respective suppliers.

2. Notice on This Instruction Manual

(1) The information contained in this manual is subject to

change without notice for product improvement.

(2) This manual is copyrighted by Hitachi High-Technologies

(3) No part of this manual may be reproduced or transmitted in

any form or by any means without the express written permission of Hitachi High-Technologies Corporation.

- 7 -

1.1 Isocratic System

1.1

1. OUTLINE

The liquid chromatograph system consists of a pump for feeding an eluent, a sample introducing section (autosampler, manual injector) for injecting a sample, a column for chromatographic separation of the injected sample, a column oven for maintaining the column at a constant temperature, and a detector section for detecting a signal of a separated component of the sample. In addition, a degasser for deaerating an eluent, a gradient device for varying a mixing ratio of eluents with time, and other optional devices are incorporated in the system.

The isocratic system has a simple configuration in which singlesolvent analysis is performed.

Washing solution

Degasser

Column

Eluent

System power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-2200 autosampler

Model L-2100/2130 pump

Drain

1 -

1.1 Isocratic System

(1) An eluent is fed by the pump through the degasser.

(2) A sample is injected from the autosampler.

(3) The injected sample is separated through the column which

is maintained at a constant temperature by the column oven.

(4) Each component separated from the sample is then

detected as a signal by the detector.

1 -

1.2 Low-Pressure Gradient System

pressure

In the low-pressure gradient system, two or more solvents are mixed in its low-pressure section from which a liquid feed is performed by a single pump. The composition of an eluent is made to vary with time in chromatographic separation.

Washing solution

Mixer

Degasser

(1) Each of plural eluents is fed from the degasser to the low-

pressure unit.

(2) In the low-pressure unit, the eluents are mixed at a pre-

specified ratio.

(3) Through the mixer, the mixture eluent is delivered.

(4) A sample is injected by the autosampler.

(5) The injected sample is separated through the column which

is maintained at a constant temperature by the column oven.

(6) Each component separated from the sample is detected as

a signal by the detector.

Column

1.2

Eluent

System power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-2200 autosampler

Lowgradient unit

Model L-2100/2130 pump

Drain

1 -

1.3 High-Pressure Gradient System

2130 pump

1.3 High-Pressure Gradient System

In the high-pressure gradient system, two or more solvents are fed by respective pumps and mixed in the high-pressure section for liquid delivery.

System power supply unit

Model L-2100/ 2130 pump

Model L-2100/

Degasser

Washing solution

Mixer

(1) From the degasser, each of plural eluents is applied to the

(2) The plural eluents are fed at a flow rate corresponding to a

(3) The plural eluents thus fed are mixed in the high-pressure

(4) A sample is injected by the autosampler.

(5) The injected sample is separated through the column which

(6) Each component separated from the sample is detected as

Eluent

pump.

pre-specified mixing ratio.

gradient section. Through the mixer, the mixture eluent is delivered.

is maintained at a constant temperature by the column oven.

a signal by the detector.

Column

Model L-2400 UV detector

Model L-2300 column oven

Model L-2200 autosampler

Drain

1 -

2.1 Unpacking

2.1

2. INSTALLATION

CAUTION

Beware of heavy objects!

This instrument is as heavy as 15 kg. When carrying this instrument, exercise care not to incur injury by dropping it off accidentally. Be sure to hold the front and rear parts of the instrument securely when moving it.

Carefully unpack the shipping case, take out module units and standard accessories, and place them on a sturdy, level desk or bench.

NOTICE:

Installation of this instrument shall be carried out by

or under supervision of qualified service personnel of Hitachi High-Technologies Corporation or its authorized service agent. Before installation of this instrument, the customer shall make preparations for satisfying the installation requirements in accordance with this manual. When relocation of this instrument becomes necessary after initial installation (delivery), please notify your local Hitachi High-Technologies sales representative or service office.

2 -

2.2 Installation Place

When selecting an installation place for this instrument, be sure to check the following conditions:

(1) Operating Ambient Temperature

4 to 35 °C Temperature variations during measurement should be minimized. Take sufficient care to prevent dew condensation.

(2) Operating Ambient Humidity

45 to 85% RH

(3) Atmosphere

(a) Well ventilated.

(b) Free from acidic or alkaline gases that could corrode

metals significantly.

that could affect the paint coating on the instrument.

(4) Other Requirements

(a) Not exposed to direct sunlight.

Exposure to direct sunlight could result in deteriorated performance or discoloration of the instrument. Avoid installing the instrument near a window of the laboratory room.

(b) Not exposed to drafts directly.

(d) Apart from a heat source such as a gas burner,

electric heater, or oven.

(e) Apart from a strong magnetism source such as an

electronic welder, high-frequency electronic furnace, or voltage transformer.

2 -

(f) Free from an amount of dust and dirt.

Contamination with dust and dirt could deteriorate the performance of the instrument.

(g) No abrupt fluctuations in power voltage.

Fluctuations in power voltage could cause noise in analytical data.

(h) Do not connect the instrument to a power line used by

an electric device that is not equipped with a noise suppressor circuit (e.g., stirrer, vibrator).

2.2

2 -

2.3 Items to be Prepared by the User

2.3.1 Power Supply

(1) Power voltage : 100 to 240 V AC

(2) Frequency : 50 or 60 Hz

(3) Power

consumption : 500 VA or more in total system

(4) Grounding : Grounding connection of Electric

Fluctuation.......Within ±10% with respect

to rated voltage

Fluctuation.......Within ±0.5 Hz with respect

to rated frequency

Provide power supply sufficient for combinational use with other devices.

Installation Technical Standard Class 3 or higher should be provided.

WARNING

Ground Properly to Prevent Electric Shock Hazard!

Be sure to use the power cable supplied with the

•

instrument. Use of a different power cable could result in an electric shock hazard.

This instrument is of a "plug-connected type" specified in

•

European Norm EN61010-1, so connect the power cable to a grounded three-wire outlet.

If a grounded three-wire outlet is not available, the power

•

cable may be connected to a two-wire outlet through an adapter plug. In this case, be sure to provide proper grounding connection as required.

2 -

2.3.2

Ground resistance: Less than 100 ohms

More than 750 mm under ground

300 mm

Copper plate

2 mm

10 mm

2.3.2 Installation Space

Power outlet

Table tap with ground cable

Ground terminal

Power cord

L-2100/2130

Ground

Ground wire (green)

Connector

For installation of this system, it is required to provide a space of at least 640 mm (width) × 550 mm (depth). Prepare a sturdy, level desk or bench capable of supporting a weight load of at least 150 kg. On the rear of the main unit, allow at least 100 mm of clearance.

NOTICE

2.4 Checking the Contents

After unpacking, check the contents of each case against the packing list attached to it.

: Installation of the instrument shall be carried out by

or under supervision of qualified service personnel of the manufacturer or its authorized service agent. Before installation of the instrument, the customer is requested to prepare necessary utilities referring to this manual. When relocation of the instrument becomes necessary after initial installation (delivery), please notify your local Hitachi High-Technologies sales representative or service office.

2 -

2.5 Assembly

2.5 Assembling

2.5.1 Power Supply Unit

The L-2000 Series system is provided with a system power supply unit which is capable of connecting two pumps, one autosampler, and one detector. The column oven has its own power cord. In an instance where provision of a single system power supply unit is insufficient for the use of two detectors, use an AC adapter suitable for each instrument (50 W AC adapter for the pump/autosampler, 100 W AC adapter for the detector). The power cable from the system power supply unit and the AC adapter power cable should be connected to the power connector equipped on the left rear side of the instrument.

Rear of instrument

e-Line connector

Power connector

2 -

(1) Isocratic System/Low-Pressure Gradient System

pump

To AC power

source

Connect the power cables of the system power supply unit and column oven to AC power source. For the L-2100/ 2130 pump and L-2100 pump, connect the 50 W cable from the system power supply unit to the instrument power connector. For the L-2400 UV detector, connect the 100 W cable from the system power supply unit to the instrument power connector.

System power supply nit

Model L-2400 UV detector

100 W cable

Model L-2300 column oven

Model L-2100 pump

50 W cable

Model L-2100/2130

2.5.2

2 -

source

2.5 Assembly

Model L-2400 UV detector

L-2300 column oven

Model L-2100 pump

2.5.3 Wiring Arrangement

(2) High-Pressure Gradient System

100 W cable

System power supply unit

50 W cable

Model L-2100/ 2130 pump

To AC power

In the L-2000 Series, connect the e-Line cable to the e-Line connector equipped on the left rear of each instrument for instrument-to-instrument wiring connection.

Rear of instrument

e-Line connector

Power connector

2 -

2100 pump

Model L-2300 column oven

2100 pump

ctor

Model L-2400 UV dete

Model L-2300 column oven

Model L-

(1) Isocratic System/Low-Pressure Gradient System

System power supply unit

Model L-2400 UV detector

e-Line cable

Model L-

e-Line cable

Model L-2100/2130 pump

e-Line cable

(2) High-Pressure Gradient System

e-Line cable

System power supply unit

e-Line cable

Model L-2100/ 2130 pump

2.5.3

2 -

Run tubes from the pump to the autosampler,

Run inlet tubing to the pump and waste

2.5 Assembly

2.5.4 Piping Arrangement

In the L-2000 Series, provide inlet tubing to the pump and waste solution tubing through the right piping section of the drain reservoir. Provide tubing from the pump to the autosampler, column and detector through the center piping section of the drain reservoir.

Drain reservoir

Center Piping Section column and detector.

Right Piping Section solution tubing.

2 - 10

(1) Isocratic System

2100 pump

2100/2130 pump

Connect the inlet tube of eluent to the IN port of the degasser. Connect the OUT port of the degasser to the inlet of the pump. Connect the drain valve of the pump to port 1 of the injection valve of the autosampler. Connect port 2 of the injection valve to the column inlet. Connect the column outlet to the inlet tube of the detector cell. For washing solution, provide connection to port 1 of the syringe valve of the autosampler through the degasser.

Washing solution

Model L-2400 UV detector

Model L-2300 column oven

Model L-

Degasser

Drain valve

Eluent Inlet tube

Detector cell

Column Injection valve

Syringe valve

Drain

2.5.4

2 - 11

2100 pump

2.5 Assembly

(2) Low-Pressure Gradient System

Connect the inlet tube of eluent to the IN port of the degasser. Connect the OUT port of the degasser to the IN port of the proportioning valve. Connect the OUT port of the proportioning valve to the four-way joint. Connect the mixer of the pump to port 1 of the injection valve of the autosampler. Connect port 2 of the injection valve to the column inlet. Connect the column outlet to the inlet tube of the detector cell. For washing solution, provide connection to port 1 of the syringe valve of the autosampler through the degasser.

Washing solution

system power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-

Drain valve

L-2100/2130 pump

Degasser

Mixer

Proportioning valve

Eluent

Inlet tube

Detector cell

Column

Injection valve

Syringe valve

Drain

Four-way joint

2 - 12

System power supply

Washing solution

unit

Model L-2100/ 2130 pump

2.5.5 Module Unit Operating Procedure

2.5.5

(3) High-Pressure Gradient System

Connect the inlet tube of eluent to the IN port of the degasser. Connect the OUT port of the degasser to the IN port of the pump. Connect the drain valve of the pump to the mixer. Connect the mixer of the pump to port 1 of the injection valve of the autosampler. Connect port 2 of the injection valve to the column inlet. Connect the column outlet to the inlet tube of the detector cell. For washing solution, provide connection to port 1 of the syringe valve of the autosampler through the degasser.

Inlet tube

Eluent

Column

Detector cell

Model L-2400 UV detector

Model L-2300 column oven

Injection valve

Syringe valve

Model L-2100 pump

Drain valve

Drain

Where the chromato data station is not used for controlling operations, each module can be operated using the optional UI pad. This section describes how to use the UI pad for operations. The UI pad is provided with common control keys available for different modules and unique control keys for particular modules. The functions of the common control keys are mentioned below.

2 - 13

SET UP

2.5 Assembly

(1) Using the UI Pad

(a) Functions of common control keys

INSERT

PROG GLP UTLTY

7 8 9 0

4 5 6

1 2 3

LOCK OFF

ESC

DEL

CL ENT

Key Designation

UTLTY Starts instrument parameter setting. GLP Starts GLP function setting. Available for displaying a logbook of component

parts of each module, triggering a reset, and locking the UI pad keys. PROG Starts program editing. ESC Returns control to the monitor window. DEL Deletes a cursor-pointed step.

This key is effective only in program editing.

When the cursor is positioned on the TIME input part, pressing the DEL key

removes a step pointed by the cursor. When the cursor is positioned on

another input part, pressing the DEL key removes an item pointed by the cursor.

Pressing the ESC key on a window where "-" is indicated returns control to the

monitor window, causing cancellation of a DEL key action.

• Pump/detector time program When the DEL key is pressed, "-" is indicated. This deletion can then be defined by pressing the ENT key.

• Autosampler sequential program When the DEL key is pressed, the relevant step number is deleted and "-" is indicated. This deletion can then be defined by pressing the ENT key.

CL Clears an input numeric value during input (after a numeric value or the decimal

point is entered; before the

ENT

The cursor is indicated at the top of the input part. The previous input value is

presented as a preset value. ENT Defines the current entry of a parameter. INSERT Use this key in program editing.

• Pump/detector A new line is presented following the last line of the current program.

• Autosampler A new step is inserted.

(Arrows)

(1) Monitor window

key : Goes to the next window.

•

key : Goes back to the previous window.

•

(2) Program editing

key : The next line is presented. Pressing this key at the last line

•

causes the first line to appear in a circular fashion.

key : The previous line is presented. Pressing this key at the first

•

line causes the last line to appear in a circular fashion.

Function

key is pressed).

2 - 14

(b) Contents on screen

The parameter input window and the program setup window are described below.

1) Input of a single numeric value:

Parameter name Input range

PROGRAM No. (1 - 9)

Input part

2) Input of plural numeric values

Parameter name

Input item

LAMP CHANGE (MM, DD, YYYY)

<1 - 12>

Input range Input part

3) Selection of a numeric value:

Parameter name

Selectable items

TIME CONSTANT (0.1s = 1, 0.5s = 2, 2.0s = 3, 3 4.0s = 4, 8.0s = 5)

Input part

4) Program setup window:

Input item

NO. R.NO VIALS INJ.VOL INJ/VIAL S.TIME 1 1 80 50 1 10.0

Input part

2.5.5

2 - 15

2.5 Assembly

1) The cursor is indicated at the top of the input part. The previous numeric value is presented in the input part.

2) Numeric and decimal point key inputs are accepted in each allowable range.

3) To define an input numeric value, press the ENT key. The cursor will then move to the next item. If an erroneous numeric value is input, the cursor is brought back to the top of the input part with the input numeric value displayed. In this state, you are prompted to enter a correct numeric value. Pressing the ESC key brings you back to the initial window (monitor window). If the ESC key is pressed during numeric key input, the parameter being entered is discarded (the previous numeric value is set).

NOTICE:

At power-on, a default number is indicated in each

item selection part.

Pressing the CL key in the input part clears its contents. The cursor is then brought back to the top of the input part. In program editing, pressing the DEL key in the input part removes an item pointed by the cursor. If the ESC key is pressed in the course of setting plural parameters, the items preceding the press of the ESC key remain intact.

2 - 16

(2) Mounting the UI Pad

(a) Remove the front panel from the instrument.

Front panel

(b) Remove the metal lid from the instrument.

Meal cover

2.5.5

2 - 17

2.5 Assembly

lid.

Metal cover

Flexible tube

UI pad

(d) Turn up the lid of the connector, and insert the flexible

tube of the UI pad as far as it will go.

Connector

Flexible tube

UI pad

2 - 18

+ 68 hidden pages

Hitachi L-2100 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual