Hitachi L-2100 Instruction Manual

INSTRUCTION MANUAL

FOR

MODEL L-2100 PUMP



Copyright

Hitachi High-Technologies Corporation 2002. All rights reserved. Printed in Japan. Part No. 890-9101

PREFACE

Thank you very much for purchasing Hitachi Model L-2100/2130
pump, which has been specifically designed for automatic
analysis in a liquid chromatograph system.
The Model L-2100/2130 pump is intended for use by persons
having a basic knowledge of chemical analysis.
Remember that improper use of analytical instruments,
chemicals or samples would result not only in wrong analytical
data but also in consequences adverse to safety.
Carefully read this instruction manual before attempting
operation. For proper use of the pump, please acquaint yourself
with it.

ABOUT THIS MANUAL

The operating procedures and maintenance/checkup instructions
for the L-2100/2130 pump are described in this manual.
First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the
beginning of this manual for ensuring safety in operation of the
pump.
If installation of the pump is not yet completed, refer to Section 2.
Before attempting operation, read through Sections 3 and 4 to
become acquainted with the structure of the instrument and its
basic operations.

- 1 -

- 2 -

IMPORTANT

Precautions on Electromagnetic Wave Interference

1. Possible Electromagnetic Wave Interference Caused by This Instrument

Where this instrument is used in a residential area or an
adjacent area thereto, it may cause interference to radio and
television reception.
To prevent this, use the specified system connection cables in
strict accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave
interference caused by it if the specified cables are connected
properly.
However, there is no guarantee that electromagnetic wave
interference will not be caused by the instrument.
If the instrument does cause interference to radio or television
reception, which can be determined by turning the instrument off
and on, the user is encouraged to try to correct the interference
by one or more of the following measures:

Reorient the antenna of the radio/television receiver.

•

Increase separation between the instrument and the radio/TV

•

receiver.

Connect the instrument to an outlet on a circuit different from

•

that to which the radio/TV receiver is connected.

2. Possible Electromagnetic Wave Interference Affecting This Instrument

Where this instrument is used near an intense electromagnetic
source, interference noise may be given to the instrument to
incur an adverse effect on its performance or functionality.
To present this, use the specified system connection cables in
strict accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave
interference affecting it if the specified cables are connected
properly.
However, there is no guarantee that electromagnetic wave
interference will not occur in the instrument. If the instrument
does incur electromagnetic wave interference, which can be
determined by turning on and off possible sources of
electromagnetic interference nearby, the user is encouraged to
try to correct the interference by one or more of the following
measures:

- 3 -

Warranty on Product

Reorient the instrument.

•

Increase separation between the instrument and possible

•

sources of electromagnetic interference.

Connect the instrument to an outlet on a circuit different from

•

that to which possible sources of electromagnetic interference
are connected.

Check that any other device connected with the instrument is

•

not affected by electromagnetic interference.

The Model L-2100 pump is warranted to be free from defects in
material or workmanship under normal use within the product
specifications indicated in this manual and under conditions
given below. This warranty is void if the instrument is not used
according to the instruction manual.

(1) Scope of Warranty

Any parts which prove to be defective in design or
workmanship during the warranty period will be repaired,
adjusted or replaced without charge. A substitute part may
be used for repair, or replacement with an equivalent
product may be made instead of repair. Such system
components as a personal computer and printer to be
updated frequently for improvement may not be available in
original versions at the time of replacement. Note that this
warranty does not apply to the instrument if modified by the
user or resold without permission from the manufacturer,
consumable parts, and any failure of lifetime-expired parts.

(2) Warranty Period

One year from the date of initial installation

(3) Availability of Technical Support Service

Technical support service for this instrument is available
within regular working hours on workdays specified by
Hitachi High-Technologies Corporation.

- 4 -

(4) Limitations and Exclusions on Warranty

Note that the following cases are excluded from the scope
of this warranty.

(a) Failure due to operation at a place not meeting the

installation requirements specified by Hitachi High­Technologies Corporation.

(b) Failure due to power supply voltage/frequency other

than specified by Hitachi High-Technologies
Corporation or due to abnormality in power supply.

(c) Corrosion or deterioration of the tubing due to

impurities contained in reagent, gas, air or cooling
water supplied by the user.

(d) Corrosion of the electric circuits or deterioration of the

optical elements due to highly corrosive atmospheric
gas.

(e) Failure due to use of hardware, software or spare parts

other than specified by Hitachi High-Technologies
Corporation.

(f) Failure due to improper handling or maintenance by

the user.

(g) Failure due to maintenance or repair by a service

agent not approved or authorized by Hitachi High­Technologies Corporation.

(h) After disposal of this instrument, or after its resale

without Hitachi High-Technologies Corporation
approval.

(i) Failure due to relocation or transport after initial

installation.

(j) Failure due to disassembly, modification or relocation

not approved by Hitachi High-Technologies
Corporation.

(k) Consumables, and failure of parts that have reached

the end of specified useful life.

(l) Failure of parts excluded from the warranty in the

instruction manual or other documents.

(m) Failure due to acts of God, including fire, earthquake,

storm, flood, lightning, social disturbance, riot, crime,
insurrection, war (declared or undeclared), radioactive
pollution, contamination with harmful substance, etc.

(n) Failure of the hardware, or damage to the system

software, application software, data or hard disk due to
computer virus infection.

- 5 -

(o) Failure of the personal computer connected with the

instrument, or damage to the system software,
application software, data or hard disk due to power
interruption or momentary power voltage drop caused
by lightning.

(p) Failure of the personal computer connected with the

instrument, or damage to the system software,
application software, data or hard disk due to
disconnection of main power to the personal computer
without taking the specified normal shutdown
procedure.

(5) Disclaimer of Warranty

THE MANUFACTURER MAKES NO WARRANTIES,
EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED
HEREIN, INCLUDING WITHOUT LIMITATION THEREOF,
WARRANTIES AS TO MARKETABILITY,
MERCHANTABILITY, FOR A PARTICULAR PURPOSE
OR USE, OR AGAINST INFRINGEMENT OF ANY
PATENT. IN NO EVENT SHALL THE MANUFACTURER
BE LIABLE FOR ANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY NATURE, OR
LOSSES OR EXPENSES RESULTING FROM ANY
DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE
GIVEN BY THE MANUFACTURER, ITS DEALERS,
DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL
CREATE A WARRANTY OR IN ANY WAY INCREASE
THE SCOPE OF THIS WARRANTY.

(6) Warranty Card

The warranty card will not be reissued. Keep it in a safe
place.

Installation, Relocation and After-sale Technical Service

Installation of the instrument shall be carried out by or under
supervision of qualified service personnel of the manufacturer or
its authorized service agent.
Before installation of the instrument, the customer is requested
to prepare necessary utilities referring to this manual.
When relocation of the instrument becomes necessary after
initial installation (delivery), please notify your local Hitachi High­Technologies sales representative or service office.

- 6 -

Technical Seminars and Training for Customers

For the customers to acquire in-depth understanding of the
analytical instruments, technical seminars and customer training
courses are available at Hitachi High-Technologies Corporation
or your site. For further information, contact your local Hitachi
High-Technologies Corporation sales representative.
(The technical seminars and customer training courses are
available on a chargeable basis.)

Other Precautions

1. Handling of Chemicals and Samples

(1) The user is responsible for following relevant legal

standards and regulations in the handling, storage and
discarding of chemicals and samples used in analytical
operations of the instrument.

(2) Reagents, standard solutions and accuracy-control

samples shall be handled, stored and discarded as
instructed by the respective suppliers.

2. Notice on This Instruction Manual

(1) The information contained in this manual is subject to

change without notice for product improvement.

(2) This manual is copyrighted by Hitachi High-Technologies

Corporation with all rights reserved.

(3) No part of this manual may be reproduced or transmitted in

any form or by any means without the express written
permission of Hitachi High-Technologies Corporation.

- 7 -

1

1.1 Isocratic System

1.1

1. OUTLINE

The liquid chromatograph system consists of a pump for feeding
an eluent, a sample introducing section (autosampler, manual
injector) for injecting a sample, a column for chromatographic
separation of the injected sample, a column oven for maintaining
the column at a constant temperature, and a detector section for
detecting a signal of a separated component of the sample.
In addition, a degasser for deaerating an eluent, a gradient
device for varying a mixing ratio of eluents with time, and other
optional devices are incorporated in the system.

The isocratic system has a simple configuration in which single­solvent analysis is performed.

Washing solution

Degasser

Column

Eluent

System power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-2200 autosampler

Model L-2100/2130 pump

Drain

1 -

2

1.1 Isocratic System

(1) An eluent is fed by the pump through the degasser.

(2) A sample is injected from the autosampler.

(3) The injected sample is separated through the column which

is maintained at a constant temperature by the column
oven.

(4) Each component separated from the sample is then

detected as a signal by the detector.

1 -

3

1.2 Low-Pressure Gradient System

pressure

In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation.

Washing solution

Mixer

Degasser

(1) Each of plural eluents is fed from the degasser to the low-

pressure unit.

(2) In the low-pressure unit, the eluents are mixed at a pre-

specified ratio.

(3) Through the mixer, the mixture eluent is delivered.

(4) A sample is injected by the autosampler.

(5) The injected sample is separated through the column which

is maintained at a constant temperature by the column
oven.

(6) Each component separated from the sample is detected as

a signal by the detector.

Column

1.2

Eluent

System power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-2200 autosampler

Low­gradient unit

Model L-2100/2130 pump

Drain

1 -

4

1.3 High-Pressure Gradient System

2130 pump

1.3 High-Pressure Gradient System

In the high-pressure gradient system, two or more solvents are
fed by respective pumps and mixed in the high-pressure section
for liquid delivery.

System power
supply unit

Model L-2100/
2130 pump

Model L-2100/

Degasser

Washing
solution

Mixer

(1) From the degasser, each of plural eluents is applied to the

(2) The plural eluents are fed at a flow rate corresponding to a

(3) The plural eluents thus fed are mixed in the high-pressure

(4) A sample is injected by the autosampler.

(5) The injected sample is separated through the column which

(6) Each component separated from the sample is detected as

Eluent

pump.

pre-specified mixing ratio.

gradient section. Through the mixer, the mixture eluent is
delivered.

is maintained at a constant temperature by the column
oven.

a signal by the detector.

Column

Model L-2400
UV detector

Model L-2300
column oven

Model L-2200
autosampler

Drain

1 -

1

2.1 Unpacking

2.1

2. INSTALLATION

CAUTION

Beware of heavy objects!

This instrument is as heavy as 15 kg.
When carrying this instrument, exercise care not to incur injury
by dropping it off accidentally. Be sure to hold the front and
rear parts of the instrument securely when moving it.

Carefully unpack the shipping case, take out module units and
standard accessories, and place them on a sturdy, level desk or
bench.

NOTICE:

Installation of this instrument shall be carried out by

or under supervision of qualified service personnel
of Hitachi High-Technologies Corporation or its
authorized service agent.
Before installation of this instrument, the customer
shall make preparations for satisfying the
installation requirements in accordance with this
manual.
When relocation of this instrument becomes
necessary after initial installation (delivery), please
notify your local Hitachi High-Technologies sales
representative or service office.

2 -

2

2.2 Installation Place

2.2 Installation Place

When selecting an installation place for this instrument, be sure
to check the following conditions:

(1) Operating Ambient Temperature

4 to 35 °C
Temperature variations during measurement should be
minimized. Take sufficient care to prevent dew
condensation.

(2) Operating Ambient Humidity

45 to 85% RH

(3) Atmosphere

(a) Well ventilated.

(b) Free from acidic or alkaline gases that could corrode

metals significantly.

(c) Free from vapors of solvents (e.g., benzene, thinner)

that could affect the paint coating on the instrument.

(4) Other Requirements

(a) Not exposed to direct sunlight.

Exposure to direct sunlight could result in deteriorated
performance or discoloration of the instrument.
Avoid installing the instrument near a window of the
laboratory room.

(b) Not exposed to drafts directly.

(c) Free from intense vibrations and shocks.

(d) Apart from a heat source such as a gas burner,

electric heater, or oven.

(e) Apart from a strong magnetism source such as an

electronic welder, high-frequency electronic furnace, or
voltage transformer.

2 -

3

(f) Free from an amount of dust and dirt.

Contamination with dust and dirt could deteriorate the
performance of the instrument.

(g) No abrupt fluctuations in power voltage.

Fluctuations in power voltage could cause noise in
analytical data.

(h) Do not connect the instrument to a power line used by

an electric device that is not equipped with a noise
suppressor circuit (e.g., stirrer, vibrator).

2.2

2 -

4

2.3 Items to be Prepared by the User

2.3 Items to be Prepared by the User

2.3.1 Power Supply

(1) Power voltage : 100 to 240 V AC

(2) Frequency : 50 or 60 Hz

(3) Power

consumption : 500 VA or more in total system

(4) Grounding : Grounding connection of Electric

Fluctuation.......Within ±10% with respect

to rated voltage

Fluctuation.......Within ±0.5 Hz with respect

to rated frequency

Provide power supply sufficient for
combinational use with other devices.

Installation Technical Standard Class 3 or
higher should be provided.

WARNING

Ground Properly to Prevent Electric Shock Hazard!

Be sure to use the power cable supplied with the

•

instrument. Use of a different power cable could result in
an electric shock hazard.

This instrument is of a "plug-connected type" specified in

•

European Norm EN61010-1, so connect the power cable to
a grounded three-wire outlet.

If a grounded three-wire outlet is not available, the power

•

cable may be connected to a two-wire outlet through an
adapter plug. In this case, be sure to provide proper
grounding connection as required.

2 -

5

2.3.2

Ground resistance:
Less than 100 ohms

More than
750 mm
under ground

300 mm

Copper plate

2 mm

10 mm

2.3.2 Installation Space

Power outlet

Table tap with
ground cable

Ground
terminal

Power cord

L-2100/2130

Ground

Ground wire
(green)

Connector

For installation of this system, it is required to provide a space of
at least 640 mm (width) × 550 mm (depth). Prepare a sturdy,
level desk or bench capable of supporting a weight load of at
least 150 kg. On the rear of the main unit, allow at least
100 mm of clearance.

NOTICE

2.4 Checking the Contents

After unpacking, check the contents of each case against the
packing list attached to it.

: Installation of the instrument shall be carried out by

or under supervision of qualified service personnel
of the manufacturer or its authorized service agent.
Before installation of the instrument, the customer
is requested to prepare necessary utilities referring
to this manual.
When relocation of the instrument becomes
necessary after initial installation (delivery), please
notify your local Hitachi High-Technologies sales
representative or service office.

2 -

6

2.5 Assembly

2.5 Assembling

2.5.1 Power Supply
Unit

The L-2000 Series system is provided with a system power
supply unit which is capable of connecting two pumps, one
autosampler, and one detector. The column oven has its own
power cord. In an instance where provision of a single system
power supply unit is insufficient for the use of two detectors, use
an AC adapter suitable for each instrument (50 W AC adapter
for the pump/autosampler, 100 W AC adapter for the detector).
The power cable from the system power supply unit and the AC
adapter power cable should be connected to the power
connector equipped on the left rear side of the instrument.

Rear of
instrument

e-Line connector

Power connector

2 -

7

(1) Isocratic System/Low-Pressure Gradient System

pump

To AC power

source

Connect the power cables of the system power supply unit
and column oven to AC power source. For the L-2100/
2130 pump and L-2100 pump, connect the 50 W cable
from the system power supply unit to the instrument power
connector. For the L-2400 UV detector, connect the 100
W cable from the system power supply unit to the
instrument power connector.

System power
supply nit

Model L-2400 UV
detector

100 W cable

Model L-2300
column oven

Model L-2100
pump

50 W cable

Model L-2100/2130

2.5.2

2 -

8

source

2.5 Assembly

Model L-2400
UV detector

L-2300 column oven

Model L-2100
pump

2.5.3 Wiring
Arrangement

(2) High-Pressure Gradient System

Connect the power cables of the system power supply unit
and column oven to AC power source. For the L-2100/
2130 pump and L-2100 pump, connect the 50 W cable
from the system power supply unit to the instrument power
connector. For the L-2400 UV detector, connect the 100
W cable from the system power supply unit to the
instrument power connector.

100 W cable

System power supply unit

50 W cable

Model L-2100/
2130 pump

Model L-2100/
2130 pump

To AC power

In the L-2000 Series, connect the e-Line cable to the e-Line
connector equipped on the left rear of each instrument for
instrument-to-instrument wiring connection.

Rear of
instrument

e-Line connector

Power connector

2 -

9

2100 pump

Model L-2300 column oven

2100 pump

ctor

Model L-2400 UV dete

Model L-2300 column oven

Model L-

(1) Isocratic System/Low-Pressure Gradient System

System power supply unit

Model L-2400 UV detector

e-Line cable

Model L-

e-Line cable

Model L-2100/2130 pump

e-Line cable

(2) High-Pressure Gradient System

e-Line cable

System power
supply unit

e-Line cable

e-Line cable

Model L-2100/
2130 pump

2.5.3

2 -

Run tubes from the pump to the autosampler,

Run inlet tubing to the pump and waste

2.5 Assembly

2.5.4 Piping
Arrangement

In the L-2000 Series, provide inlet tubing to the pump and waste
solution tubing through the right piping section of the drain
reservoir. Provide tubing from the pump to the autosampler,
column and detector through the center piping section of the
drain reservoir.

Drain reservoir

Center Piping Section
column and detector.

Right Piping Section
solution tubing.

2 - 10

(1) Isocratic System

2100 pump

2100/2130 pump

Connect the inlet tube of eluent to the IN port of the
degasser. Connect the OUT port of the degasser to the
inlet of the pump. Connect the drain valve of the pump to
port 1 of the injection valve of the autosampler.
Connect port 2 of the injection valve to the column inlet.
Connect the column outlet to the inlet tube of the detector
cell. For washing solution, provide connection to port 1 of
the syringe valve of the autosampler through the degasser.

Washing solution

Model L-2400 UV detector

Model L-2300 column oven

Model L-

Model L-

Degasser

Drain valve

Eluent
Inlet tube

Detector cell

Column
Injection valve

Syringe valve

Drain

2.5.4

2 - 11

2100 pump

2.5 Assembly

(2) Low-Pressure Gradient System

Connect the inlet tube of eluent to the IN port of the
degasser. Connect the OUT port of the degasser to the IN
port of the proportioning valve. Connect the OUT port of
the proportioning valve to the four-way joint.
Connect the mixer of the pump to port 1 of the injection
valve of the autosampler. Connect port 2 of the injection
valve to the column inlet. Connect the column outlet to the
inlet tube of the detector cell. For washing solution,
provide connection to port 1 of the syringe valve of the
autosampler through the degasser.

Washing solution

system power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-

Drain valve

L-2100/2130 pump

Degasser

Mixer

Proportioning valve

Eluent

Inlet tube

Detector cell

Column

Injection valve

Syringe valve

Drain

Four-way joint

2 - 12

System power supply

Washing solution

unit

Model L-2100/
2130 pump

2.5.5 Module Unit
Operating
Procedure

2.5.5

(3) High-Pressure Gradient System

Connect the inlet tube of eluent to the IN port of the
degasser. Connect the OUT port of the degasser to the IN
port of the pump. Connect the drain valve of the pump to
the mixer. Connect the mixer of the pump to port 1 of the
injection valve of the autosampler. Connect port 2 of the
injection valve to the column inlet. Connect the column
outlet to the inlet tube of the detector cell.
For washing solution, provide connection to port 1 of the
syringe valve of the autosampler through the degasser.

Inlet tube

Eluent

Column

Detector cell

Model L-2400 UV detector

Model L-2300 column oven

Injection valve

Syringe valve

Model L-2100 pump

Drain valve

Drain

Where the chromato data station is not used for controlling
operations, each module can be operated using the optional UI
pad.
This section describes how to use the UI pad for operations.
The UI pad is provided with common control keys available for
different modules and unique control keys for particular modules.
The functions of the common control keys are mentioned below.

2 - 13

SET UP

2.5 Assembly

(1) Using the UI Pad

(a) Functions of common control keys

INSERT

PROG GLP UTLTY

7 8 9 0

4 5 6

1 2 3

LOCK OFF

ESC

DEL

CL ENT

Key Designation

UTLTY Starts instrument parameter setting.
GLP Starts GLP function setting. Available for displaying a logbook of component

parts of each module, triggering a reset, and locking the UI pad keys.
PROG Starts program editing.
ESC Returns control to the monitor window.
DEL Deletes a cursor-pointed step.

This key is effective only in program editing.

When the cursor is positioned on the TIME input part, pressing the DEL key

removes a step pointed by the cursor. When the cursor is positioned on

another input part, pressing the DEL key removes an item pointed by the cursor.

Pressing the ESC key on a window where "-" is indicated returns control to the

monitor window, causing cancellation of a DEL key action.

• Pump/detector time program
When the DEL key is pressed, "-" is indicated. This deletion can then be
defined by pressing the ENT key.

• Autosampler sequential program
When the DEL key is pressed, the relevant step number is deleted and "-" is
indicated. This deletion can then be defined by pressing the ENT key.

CL Clears an input numeric value during input (after a numeric value or the decimal

point is entered; before the

ENT

The cursor is indicated at the top of the input part. The previous input value is

presented as a preset value.
ENT Defines the current entry of a parameter.
INSERT Use this key in program editing.

• Pump/detector
A new line is presented following the last line of the current program.

• Autosampler
A new step is inserted.

,

(Arrows)

(1) Monitor window

key : Goes to the next window.

•

key : Goes back to the previous window.

•

(2) Program editing

key : The next line is presented. Pressing this key at the last line

•

causes the first line to appear in a circular fashion.

key : The previous line is presented. Pressing this key at the first

•

line causes the last line to appear in a circular fashion.

Function

key is pressed).

2 - 14

(b) Contents on screen

The parameter input window and the program setup
window are described below.

1) Input of a single numeric value:

Parameter name Input range

PROGRAM No. (1 - 9)

1

Input part

2) Input of plural numeric values

Parameter name

Input item

LAMP CHANGE (MM, DD, YYYY)

<1 - 12>

Input range Input part

3) Selection of a numeric value:

Parameter name

Selectable items

TIME CONSTANT (0.1s = 1, 0.5s = 2, 2.0s = 3,
3 4.0s = 4, 8.0s = 5)

Input part

4) Program setup window:

Input item

NO. R.NO VIALS INJ.VOL INJ/VIAL S.TIME
1 1 80 50 1 10.0

Input part

2.5.5

2 - 15

2.5 Assembly

(c) Numeric Value Input Procedure

1) The cursor is indicated at the top of the input part.
The previous numeric value is presented in the
input part.

2) Numeric and decimal point key inputs are
accepted in each allowable range.

3) To define an input numeric value, press the ENT
key. The cursor will then move to the next item.
If an erroneous numeric value is input, the cursor
is brought back to the top of the input part with
the input numeric value displayed. In this state,
you are prompted to enter a correct numeric value.
Pressing the ESC key brings you back to the
initial window (monitor window).
If the ESC key is pressed during numeric key
input, the parameter being entered is discarded
(the previous numeric value is set).

NOTICE:

At power-on, a default number is indicated in each

item selection part.

Pressing the CL key in the input part clears its contents.
The cursor is then brought back to the top of the input part.
In program editing, pressing the DEL key in the input part
removes an item pointed by the cursor.
If the ESC key is pressed in the course of setting plural
parameters, the items preceding the press of the ESC key
remain intact.

2 - 16

(2) Mounting the UI Pad

(a) Remove the front panel from the instrument.

Front panel

(b) Remove the metal lid from the instrument.

Meal cover

2.5.5

2 - 17

2.5 Assembly

(c) Run the flexible tube of the UI pad through the metal

lid.

Metal cover

Flexible tube

UI pad

(d) Turn up the lid of the connector, and insert the flexible

tube of the UI pad as far as it will go.

Connector

Flexible tube

UI pad

2 - 18

2.5.5

(e) Turn down the connector lid, and mount the UI pad.

Connector

Flexible tube

UI pad

(f) Attach the metal lid dedicated for the UI pad.

Flexible tube

Metal cover

UI pad

2 - 19

2.5 Assembly

(g) Set the of the UI pad to the of the instrument.

Tab

UI pad

(h) Mount the front panel dedicated for the UI pad on the

instrument.

Front panel

2 - 20

2.5.6

2.5.6 Instrument-to­Instrument
Fastening

The wiring cover is provided on the rear of each instrument.
Instrument-to-instrument fastening can be made by shifting each
wiring cover as shown below.

(1) Remove the retaining screws, and detach the wiring cover.

Wiring cover

Retaining
screw

(2) Shift the mounting position of each wiring cover.

Wiring cover

Retaining
screw

2 - 21

1

3.1 The Instrument Keypad

3.1.1 Name and
Function of Each
Part

LED

Main switch

3. FUNCTION

Right door

Drain Valve

Main switch : Turns on/off power supply.

•

Doors : Two doors are provided.

•

Drain Valve : This valve is opened for purging the system.

•

Leak sensor : Detects fluid leakage.

•

UI-PAD : A keypad required for operating the pump

•

LED : There are 4 LEDs, and each LED lights in a

•

A (green, orange) Green : POWER ON as the pump A

B (green, orange) Green : POWER ON as the pump B

PURGE (green) Green : Under purge

PROG. RUN (green) Green : Under the time program

Left door

Drain

Leak sensor

Fig. 3-1 Front View

independently (available at option).

specific color for the determined indication.

Name (color) LED Status

Orange : Pump A error

Orange : Pump B error

running

3 -

2

3.1.1

Wiring covers

Ground terminals

Pump can start by an
external signal.

I/F board mounting
space

Pump can stop by an
external signal.

Pressure value is output
to the exterior (Approx.
49 MPa)/1 V.

Wiring covers

Fig. 3-2 Rear View

Wiring covers : Upon detaching the left-side cover (as

viewed from the front), the e-Line
connector used for connection between
L-2000 series units appears.
These wiring covers are also usable as
retaining plates for other units of the
L-2000 series.

Ground terminals : Three terminals for grounding the

instrument. I/F board mounting

IF board space : Space for mounting the I/F board required

for connecting the PC (data station) and
HPLC system.

3 -

3

Contents of

CLOSE

1 s CLOSE

OPEN

Classi­fication

Output

Input

Table 3-1 Name and Function of Each Terminal at Rear Panel

Name Function

TIMER
(1 to 4)

PRESS
OUT

PUMP
ON IN

PUMP
OFF IN

Contact
turns
on/off
according
to coded
setting in
EVENT of
the
program.

Pressure
value is
output to
the
exterior.

Pump ON
from
external
system.

Pump OFF
from
external
system.

Signal

• Timer

ON: CLOSE

OFF: OPEN

• Pulse

OPEN

0 to 1 V DC

OPEN ∗

50 ms
or longer

Contents of
Processing

The ON/OFF
signal can
output to
each TIMER by
specifying an
ON/OFF EVENT
code at a
desired time
of a time
program.

1-second
CLOSE signal
can output at
each TIMER by
specifying
pulse EVENT
code at a
desired time
of a time
program.

Maximum current connectable: 10 mA

• TIMER No. and Event code

TIMER
No.

•

Pump can be stopped by specifying

00 for EVENT, and buzzer can be
rung by specifying 92.

1) Becomes OFF when the pulse is
output in the ON status.

2) OFF has priority over ON.

3) Contact output specification of
each terminal

Voltage ... 30 V DC max.
Current ... 100 mA max.

• 1 V = Approx. 49 MPa

∗ Edge jugement

OFF ON

1 10 11 12
2 20 21 22
3 30 31 32
4 40 41 42

(Approx. 7106 psi)

Remarks

Signal

3.10

Pulse

3 -

4

SET UP

PUMP

A B

C D

3.1.2 Part Name and
Function of UI
Pad (option)

PURGE

(1) Operation Keys

Each key on the UI pad (option) type operation panel is
explained here.

INSERT INITIAL

PROG GLP UTLTY

START

STOP

7 8 9 0 ESC

4 5 6

1 2 3

MANUAL

CL ENT

LOCK OFF

DEL

PUMP
ON/OFF

PURGE Starts/stops purging the flow path.

UTLTY

/

SET UP

GLP Used for confidence-level reporting.
PROG Selects, generates, edits, or deletes a pump program.

, Scroll up and down display.

START Starts the time program. You can activate the start key

STOP Stops the time program.
MANUAL Used for manual operation selection display and

ESC

/

LOCK
OFF

A,B,C,D If you are in manual status and you press the [A]

INITIAL Puts the systems to the initial setting of program

Starts/stops operation of the pump. Pressing this key
starts the pump.
Then, pressing it again stops the pump.

For specifying a purging flow rate, use the UTILITY
function.
The upper pressure limit (PRESS MAX) is set at 2.9
MPa (30 kgf/cm2), and the lower pressure limit
(PRESS MIN) is set at 0 MPa (0 kgf/cm2).
Pressing this key again stops the purging operation.

1.Used to display/set up auxiliary function conditions
(UTILITY).

• Pressure zero-point correction

• Purge flow rate setting

• Pressure unit assignment

2.For pump mode selection.
The low-pressure gradient mode, high-pressure
gradient mode, and single-liquid feed mode are
available (SET UP).

only when the pump is at the initial mode.

setting (mobile phase mixing ratio, FLOW, P-MAX, P­MIN, %B, %C, %D).

1. Pressing this key brings you from the numeric value
input state (input state in each mode) back to the
MONITOR screen.

2. For key unlocking.

key %A is set immediately to 100. [B], [C], and [D]
keys provide the same function.

whose no. is selected by [PROG] key (time program: 0
minutes)

3 -

5

CL Used for erasure of data under input. Also usable for

DEL Used for deleting a step in a sequential program.
ENT Inputs a numeral.

erasure of an error message at occurrence of an error.

NOTE: If a mistake is made during operation, press the

ESC key. In almost all cases, the initial status

returns when pressing this key.

(2) Monitor Screen

The UI pad is provided with an LCD monitor on which
instrument status can be indicated. The screen which
indicates instrument status is called a monitor screen.
Operation method of the monitor screen and example
indications on it are shown below.

(a) 1st monitor screen

This screen mainly indicates information about the
pump main frame.

TIME %A %B %C %D FLOW P RESS [ --]

0.0 100 0 0 0 0.000 0.0 1

Indication Description

TIME
%A
%B
%C
%D
FLOW
PRESS
[--]

Elapsed time of analytical program execution
Mixing ratio of liquid A
Mixing ratio of liquid B
Mixing ratio of liquid C
Mixing ratio of liquid D
Pump flow rate
Pump pressure
Pump status indication

Since two-liquid low-pressure gradient operation is performed in
this system, %C and %D are always zero.

The status indications at the upper right of the
MONITOR screen are as follows:

[GR] The gradient program is in execution.

3 -

6

[IN] Waiting for execution of analysis. A liquid is being fed.
[--] Waiting for execution of analysis. The pump is being
stopped.
[MN] The MANUAL mode is selected.
[PG] The purging operation is under execution.
[LK] The keyboard of the operation panel is locked.

(b) 2nd monitor screen

P-UNIT P.MAX P.MIN [--]
MPa 20.0 0.0 1

Indication Description

P-UNIT Indicates a unit of pressure. In this system, MPa is

P. MAX Indicates a specified upper limit of pressure. If

P. MIN Indicates a specified lower limit of pressure. If

indicated.

pressure exceeds the specified upper limit, the pump
is forced to stop.

pressure decreases below the specified lower limit,
the pump is forced to stop.

(c) 3rd monitor screen

EVENT 1 2 3 4 PUMP FLUSH [--]
OFF OFF OFF OFF A(Micro) OFF 1

Indication Description

EVENT Indicates a state of the EVENT terminals 1 to 4. ‘ON’

PUMP Indicates a pump station number. In pump station

FLUSH Always indicated as ‘OFF’ in this system.

represents that the contact is closed, and ‘OFF’
represents that the contact is open.

number assignment, PUMP A, PUMP B or PUMP C
can be specified.

3 -

7

(c) Changeover of monitor screen
The monitor screen transition is as follows. If the link program
function is OFF, the 4th monitor screen will not appear.

1) 1st MONITOR screen

TIME %A %B %C %D FLOW PRESS [--]

0.0 100 0 0 0 0.000 0.0 1

2) 2nd MONITOR screen

P-UNIT P.MAX P.MIN [ --]
MPa 20.0 0.0 1

3) 3rd MONITOR screen

EVENT 1 2 3 4 PUMP FLUSH [--]
OFF OFF OFF OFF A (Micro) OFF 1

4) 4th MONITOR screen

LINK PROG STEP CYCLE [ --]
O N 1 1/99 1

or

ESCAPE

3 -

8

3.2 Operation Status

Fig. 3-3 is a flow chart that shows the transition of operational
status. ( indicates depression of a key.)

<Turning on power>

(does not function unless the pump is on)

START/STOP

INITIAL

INIT

START/STOP

MANUAL

SET

MANUAL

RUN

MANUAL

SET

INITIAL : Programming mode status in which

system waits for start.

MANUAL : Operation is made under constant

conditions without using program.

RUN : Gradient mode is executed.

Fig. 3-3 Operational Status Transition

3 -

9

3.3 Specifications

(1) L-2100

(a) Liquid feed system:
Dual plunger type reciprocating pump( Tandem Type)
(b) Maximum operating pressure:

39.2 MPa
(c) Flow rate range

0.001 to 2.499 mL/min
(d) Pressure limit range
0 - 39.2 MPa
(e) Delivery volume of No.1 plunger stroke
25 uL/stroke
(f) Flow accuracy (at constant room temperature of
20 for water)
+/- 2 uL/min (0.005 - 0.050 mL/min, 1.0 - 39.2 MPa)
+/- 2 % (0.051 – 2.000 mL/min, 1.0 - 39.2 MPa)
+/- 4 % (2.000 - 2.499 mL/min, 1.0 - 39.2 MPa)
(g) Flow precision

0.075% RSD or 0.02min SD, whichever is greater,
based on retention time using L-2200 autosampler
(Flow 0.2 mL/min at constant room temperature of
20 for water )
(h) Pressure display accuracy
+/- 5%
(i) Constant pressure liquid feed

2.0 to 39.2 MPa (Flow range 0 to 1.25 mL/min)
(j) Material of wetted parts

SUS 316, ruby, sapphire, ceramic, PTFE,
carbon-impregnated PTFE

(k) Display data using UI-Pad (Option):

Parameters
Gradient monitor Elapsed time, mixing ratio, flow rate,

pressure, timer value, etc.
(l) Pump control mode:
Single-Liquid delivery mode
Low pressure gradient mode
High pressure gradient mode
(m) Contact input/output terminals:

Event timer; 4 channels
(n) Communication:

e-Line

OUTPUT: BUSY

INPUT: 1) GRADIENT START IN

2) ERROR IN

(o)Pressure zero adjustment:

3 -

Possible
(p) Ambient temperature :
4 to 35°C (condensation unallowable)
(q) Humidity:
45 to 85% RH
(r) Power supply:
24V DC, 21.A
(s) Dimensions:
260(W) × 500(D) × 235(H) mm
(t) Weight:
About 15 kg

(2) L-2130

(a) Liquid feed system:
Dual plunger type reciprocating pump( Tandem Type)
(b) Maximum operating pressure:

39.2 MPa
(c) Flow rate range

0.001 to 9.999 mL/min
(d) Pressure limit range
0 - 39.2 MPa
(e) Delivery volume of No.1 plunger stroke
25 uL/stroke
(f) Flow accuracy (at constant room temperature of
20 for water)
+/- 2 uL/min (0.01 - 0.10 mL/min, 1.0 - 39.2 MPa)
+/- 2 % (0.101 – 5.000 mL/min, 1.0 - 39.2 MPa)
+/- 2 % (5.001 – 8.000 mL/min, 1.0 – 19.6 MPa)
+/- 4 % (8.001 - 9.999 mL/min, 2.0 – 19.6 MPa)
(g) Flow precision

0.075% RSD or 0.02min SD, whichever is greater,
based on retention time using L-2200 autosampler
(Flow 1.0mL/min at constant room temperature of
20 for water )
(h) Pressure display accuracy
+/- 5%
(i) Constant pressure liquid feed

2.0 to 39.2 MPa (Flow range 0 to 5.0 mL/min)
(j) Material of wetted parts

SUS 316, ruby, sapphire, ceramic, PTFE,

carbon-impregnated PTFE
(k) Display data using UI-Pad (Option):

Parameters

Gradient monitor Elapsed time, mixing ratio, flow rate,

pressure, timer value, etc.

3 - 10

(l) Pump control mode:
Single-Liquid delivery mode
Low pressure gradient mode
High pressure gradient mode
(m) Contact input/output terminals:

Event timer; 4 channels
(n) Communication:

e-Line

OUTPUT: BUSY

INPUT: 1) GRADIENT START IN

2) ERROR IN
(o)Pressure zero adjustment:
Possible
(p) Ambient temperature :
4 to 35°C (condensation unallowable)
(q) Humidity:
45 to 85% RH
(r) Power supply:
24V DC, 21.A
(s) Dimensions:
260(W) × 500(D) × 235(H) mm
(t) Weight:
About 15 kg

3 - 11

1

4. OPERATION

The method of pump operation using the optional UI pad is
described here.

4.1 Preparatory Operations

Explained here is the operating method for using the L-2130
pump.

4.1.1 Setting of Pump Mode

This section shows how to set the pump in the single-liquid
delivery mode (FLOW mode).

NOTICE: Once this setting is made, it is memorized and need not
be made each time.

<POWER ON> + [(SETUP) UTILITY]

When pressing the [POWER] and [(SETUP) UTILITY]
keys simultaneously, the pump mode setting screen
appears. It may be necessary to continue pressing
[(SETUP) UTILITY] until the setting screen appears.

SETUP PUMP MODE CONTRAST S.NO.
A LOW 4 02AB-111

[ENTER]

SETUP ITEMS PUMP=1 CONTRAST =2

[1][ENTER]

PUMP A=1 B=2

[1][ENTER]

4 -

2

GR MODE LOW HIGH V. HIGH
4 FLOW 4

[4][ENTER] LOW: Low pressure gradient mode

HIGH: High pressure gradient mode

V.HIGH: High pressure gradient with

solenoid valves mode

SETUP PUMP MODE CONTRAST S.NO.
A FLOW 4 02AB-111

FLOW: Isocratic mode

<POWER OFF>
Turn the power off once.

<POWER ON>
Turn the power on again.

TIME FLOW PRESS [ ]

0.0 1.000 0.0 1

4 -

3

4.1.2 Programming Operation

This section discusses the programming of the FLOW mode.
An example is given where upper pressure limit is 20.0 MPa,
lower pressure limit is 0 MPa, analysis time is 0 min, flow rate is

1.0 mL/ min.

PROGRAM No. = 1

PRESSURE VALUE : MAX MIN MPa

TIME FLOW EVENT
(min) (ml/min) 1 2 3 4

0.0 1.000 12 22 32 42

Monitor screen

If manual setting screen is displayed, press INITIAL to
obtain the programming mode.

PROG

Program generation is started.

SET PROGRAM: No. [--]
<1-9> 1

Input program No. Valid program numbers are from 1 to

9.

MODIFY=0, NEW=1 [--]
0

Generates a new program or modifies an existing
program.

20.0 0.0

4 -

4

PRESSURE: MAX MIN [--]

20.0 0.0 1

Input the upper and lower limit pressure values.

TIME FLOW EVENT [--]

0.0 1.000 1

Input time, flow rate and event. Up to 4 entries can be
made per step for event. Input an event to be
executed within the entered time.

TIME FLOW EVENT [--]

1

A new input screen will appear, so enter time, flow
rate and event.

[ ESCAPE ]

Monitor screen

NOTICE: A maximum of 99 steps can be entered for program
Nos. 1 to 9.

NOTICE: The entered program is saved. When power is turned on

next time, the initial screen (TIME=0) of the program used just before
power was turned off will appear.

4 -

5

when power is turned off. Conditions must be set each time power

4.1.3 Manual Operation

This is used for supplying liquid under constant conditions
without using a program.

(1)Constant Flow Rate Mode

This section explains the setting of liquid delivery system at
constant flow rate. An example is given where flow rate at 1.0
mL/min, upper pressure limit at 15.0 MPa and lower pressure
limit at 0 MPa.

Monitor screen

MANUAL

MANUAL: FLOW=1, PRESS=2 [MN]

CONSTANT FLOW: FLOW MAX MIN [MN]

CONSTANT FLOW: FLOW PRESS [MN]

Monitor screen

NOTICE: The set conditions for manual operation will be erased

1

Select the constant flow rate mode.

1.000 15.0 0.0

Input the flow rate, and the upper and lower limit pressure
values.

0.000 0.0

The monitor screen will apper.

is turned on.

4 -

6

power is turned off. Conditions must be set each time power is turned

(2)Constant Pressure Mode

This section explains the setting of liquid delivery system at
constant pressure. An example is given where initial flow rate is

1.0 mL/min, at a pressure of 15.0 MPa.

Monitor screen

MANUAL

MANUAL: FLOW=1, PRESS=2 [MN]

2

Select the constant pressure mode.

CONSTANT PRESS: INITIAL FLOW PRESS [MN]

1.000 15.0

Input the flow rate, and the pressure values.

CONSTANT FLOW: FLOW PRESS [MN]

0.000 0.0

The monitor screen will apper.

Monitor screen

Before stating the pump, check that the pressure level is 0 Mpa.

NOTICE: The liquid delivery will not start unless the starting pressure
is 0 MPa

NOTICE:

The set conditions for manual operation will be erased when

on.

4 -

7

4.2 Measuring Operation

Ignition of Flammable Chemicals!

Beware of ignition hazard when using flammable chemicals such

•

as organic solvents.

Always check the following conditions. If an abnormality is

•

found, stop operation immediately.

Leakage of solvent or waste solution

•

Leakage of solvent inside the instrument

•

Inadequate ventilation of the laboratory room

•

This instrument is not explosion-proof. Although aqueous

•

solvents or organic solvents having an ignition point of 70°C or
higher are usable, do not use organic solvents having an ignition
point below 70°C.

When using flammable chemicals, be careful about possible

•

ignition due to static electricity. Particularly when using non­conductive chemicals, employ a conductive vessel made of metal
or the like and provide grounding connection correctly.

WARNING

WARNING

Explosion of Vapor from Flammable Chemicals!

If a flammable chemical such as organic solvent leaks from the

•

flow path of the instrument and its vapor concentration exceeds
the explosion limit, it may cause spontaneous combustion with
dangerously explosive results.

When using a flammable and readily volatile chemical, be sure to

•

check for leakage from the instrument flow path and ventilate the
laboratory room adequately.

4 -

8

4.2.1 Basics of Operation

When the settings are all finished, carry out operation in the
following procedure.

(1) Make sure the pump, column, detector and data
processor are properly connected.

(2) Set the mobile phase and waste solution bottle in place.

(3) Start the pump and wait until the flow path is filled with
the mobile phase.

(4) Warm up the pump, column and detector and wait until
the baseline stabilizes.

(5) Inject a sample.

(6) When analysis is finished, turn the power off and dispose
of the waste solution.

NOTICE: Before connecting a new column, make sure the flow
path is filled with mobile phase so that air won’t enter the
column.

NOTICE: The whirring sound of the pump during operation
varies depending on such operating conditions as flow rate and
pressure. Although the whirring sound may become larger
anywhere in the high-pressure range, it is not a symptom of
abnormality.

4 -

9

4.2.2 Analyzing Operation

(1) SET PROGRAM allows the generation of a maximum of 9 programs.

You can execute any of the programs selected or generated via the SET PROGRAM.
For a gradient analysis, implement the following procedure.

(a) Select [INITIAL] , and select or generate a program for eluent.
(b) Turn on each pump.
(c) Start the program.

(2) Contents of SET PROGRAM

For the SET PROGRAM, enter the upper limit pressure level, lower limit pressure
level, mixing ratio, flow rate, and event.

PROG No.=1

PRESSURE MAIN MAX MIN (MPa)

20.0 0
(a)

TIME FLOW EVENT

0.0 1.000

10.0 1.000 11 21 31 41

10.1 1.000

20.0 1.000

(b) 25.0 10 20 30 40

30.0 2.000

30.1 1.000

40.0 1.000 00 92

Fig. 3-4 Example of Program

(a) For EVENT, up to 4 event codes are settable at each time point. It is

possible to generate contact outputs at each EVENT terminal at the same
time. You can also obtain an internal action, such as a pump stop and the
ringing of a buzzer.

Example) EVENT= 1 - 1 - ENTER - 2 - 1 - ENTER

4 -

3.9

(b) In case that an event setting is desired independent of FLOW , only the event

code showed be entered. Press ENTER to skip all the entries you do not

want until you reach event column.
In the SET PROGRAM mode, the interpolation of mixing ratio, flow rate, etc. that
occurs in a time program is as follows.

Parameter Flow Rate

Interpolation Linear

TN T

N+1

TN, T

: Preset time

N+1

NOTICE: For generating a stepwise gradient program, utilize T

- TN = 0.1.

N+1

4 - 10

(1) New Generation of a Program

This section shows the generation of a new program.

SET PROG (SET PROG No.) ENTER

(MODIFY=0, NEW=1) 1 ENTER

(MAX) ENTER (MIN) ENTER

(TIME) Set time (Ti) ENTER

(FLOW) Set flow rate (Fi) ENTER

(EVENT) Event code (E1i) ENTER

Event code (E2i) ENTER

Event code (E3i) ENTER

Event code (E4i) ENTER

3 4

Upper limit pressure
level

5

6

7

7

7

7

8 Repeat steps 5 to 7 until you define all programs lines.

9 To terminate the program generation press [ESC]

2

1

No. of program to be
generated (m)

Lower limit pressure
level

4 - 11

1 Program number can be from 1 to 9.
2 When you want to define a new program, enter 1.

As a result, you will delete the previous program.
For modifying the contents of program, enter 0.

3 If pump pressure rises beyond the level set here, the pump automatically stops.
4 If pump pressure falls below the level set here, the pump automatically stops.
5 The set time can be from 0.0 to 600.0.
6 Flow rate valid entries are from 0.00 to 2.499 (L-2100). Flow rate valid entries are

from 0.00 to 9.999 (L-2130).

7 Input the event code. At the set time point, you can stop the pump, generate an

audible signal, markers, timers, etc. When properly interfaced, these signals can
be output to an external system. No display will show on the screen unless an

event is set.
8 Repeat steps 5 to 7 until you complete the program and then press [ESC] .
9 Program generation will be terminated and the monitor screen will reappear.

For confirming the input conditions, check the parameters at each time point by using
keys.

4 - 12

2) Deletion of Program

(

How to delete a program is shown next.

SET PROG (SET PROG No.)

ENTER (MODIFY=0, NEW=1) 1 ENTER ESCAPE

1

No. of program to be
deleted (m)

2 3

(3) Deletion of Step

How to delete one step in a program is shown next.

SET PROG (SET PROG No.)

1

ENTER (MODIFY=0, NEW=1) No. of program containing step to be

deleted (m)

2 3

0 ENTER (MAX) ENTER (MIN)

4 5 6 7

Lower limit pressure
level

ENTER DELETE ENTER ESCAPE

Upper limit pressure
level

4 - 13

1 Program number can be from 1 to 9.
2 The contents of the existing program will be deleted.
3 The monitor screen reappears.

1 Program number can be from 1 to 9.
2 For editing, input 0. For generating a new program, input 1.
3 If pump pressure rises beyond the level set here, the pump automatically stops.

4 If pump pressure falls below the level set here, the pump automatically stops.
.

5 Press the key until the step you want to delete appears on the screen. If display

advances beyond that step, press the

key.
6 Press the [DEL] key when the cursor is on the TIME line want to delete.
7 To finish editing, press the [ESC] key. The display should then return to the

monitor screen.

4 - 14

(4) Addition of Step

SET PROG (SET PROG No.) ENTER

(MODIFY=0, NEW=1) 0 ENTER

(MAX) ENTER (MIN) ENTER

(TIME) Time point of step added (Ti) ENTER

(FLOW) Set flow rate (Fi) ENTER

(EVENT) Event code (E1i) ENTER

Event code (E2i) ENTER

Event code (E3i) ENTER

Event code (E4i) ENTER

3 4

Upper limit pressure
level

5

6

7

7

7

7

2

1

No. of program for adding
a step (m)

Lower limit pressure
level

4 - 15

1 Program number can be from 1 to 9.
2 For editing, input 0. For generating a new program, input 1.

3 If pump pressure rises beyond the level set here, the pump automatically stops.
.

4 If pump pressure falls below the level set here, the pump automatically stops. The
.

5 Input the time point of the step you want to add.

6 Flow rate valid entries are from 0.00 to 2.499 (L-2100). Flow rate valid entries are

from 0.00 to 9.999 (L-2130).

7 Input the event code. At the set time point, you can stop the pump, generate an

audible signal, markers, times, etc. When properly interfaced, these signals can be

output to an external system. No display will show on the screen unless an event is

set.

4 - 16

(5) Correction of Step

Step correction method is explained here.

SET PROG (SET PROG No.)

1

ENTER No. of program containing step to be

deleted (m)

(MODIFY=0, NEW=1) 0 ENTER

(MAX) ENTER (MIN) ENTER

5

(TIME) Time point of step added (Ti) ENTER

(FLOW) Set flow rate (Fi) ENTER

(EVENT) Event code (E1i) ENTER

Event code (E2i) ENTER

Event code (E3i) ENTER

Event code (E4i) ENTER

3 4

Upper limit pressure
level

6

7

8

8

8

8

2

Lower limit pressure
level

4 - 17

1 Program number can be from 1 to 9.
2 For correction, input 0. For generating a new program, input 1.

3 If pump pressure rises beyond the level set here, the pump automatically stops. ]

4 . If pump pressure falls below the level set here, the pump automatically stops.

5 Press the key until the step to be corrected appears on the screen. If the display

advances beyond that step, press the key.
6 When a correction is unnecessary, press the ENTER key only. The allowable

input range is 0.00 to 2.499.

7 When a correction is unnecessary, press the ENTER key. Valid flow is from 0.00

to 2.499(L-2100). Valid flow is from 0.00 to 9.999(L-2130).

8 Enter the event code. When a correction is unnecessary, press the ENTER key.

4 - 18

(6) Deletion of Parameter

The parameter deletion method is shown next.

SET PROG (SET PROG No.)

ENTER No. of program containing parameter to

be deleted (m)

(MODIFY=0, NEW=1) 0 ENTER

(MAX) ENTER (MIN) ENTER

5 1 6 7

ENTER DELETE ENTER

3 4

Upper limit pressure
level

2

Lower limit pressure
level

4 - 19

1 Program number can be from 1 to 9.
2 For correction, input 0. For generating program, input 1.
3 If pump pressure rises beyond the level set here, the pump automatically stops.
4 If pump pressure falls below the level set here, the pump automatically stops.
5 Press the key until the step to be corrected appears on the screen. If display

advances beyond that step, press the

key.
6 Move the cursor to the parameter to be deleted.
7 To delete the parameter, press the DELETE key and then press ENTER key.

4 - 20

4.3 Setting of Utility Functions

Utility functions such as zero adjustment of pump pressure, selection of pump pressure unit
and column flushing are settable as follows.

4.3.1 Pressure Zero Adjustment

You do not need to perform a pressure zero adjustment every day. The zero point may
change due to a secular change of the pressure sensor, etc. Check the pressure zero point
once a month. If it has drifted, then stop the pump, open the drain valve and carry out the
following operation.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

1 EVENT 4 LINK= OTHERS=6

Select CONDITION .

CONDITION SET Z-ADJ=1 MOTOR-CTRL=2

1 PURGE=3, CALIBRATION=4

Select Z-ADJ (PRESS ZERO ADJUST).

PRESS ZERO ADJUST: YES=1, NO=0 [--]

(OPEN DRAIN VALVE) 0

Open the drain valve, and then enter 1.

Monitor screen

PRESS indication becomes “0” and the pressure zero adjustment is finished.

4 - 21

Pump Motor Control Method

4.3.2

Automatic flow rate control method (STANDARD) and constant flow rate control method
(FIX) are available as pump motor control methods.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

EVENT 4 LINK= OTHERS=6

Select CONDITION .

CONDITION SET Z-ADJ=1 MOTOR-CTRL=2

2 PURGE=3, CALIBRATION=4

Select MOTOR-CTRL (MOTOR CONTROL).

MOTOR CONTROL: STANDARD=1, FIX=2 [--]
1

STANDARD

Monitor screen

MOTOR CONTROL is set to STANDARD.

FIX

FIX STEP AREA
<0, 30-300> 100

Enter a FIX STEP AREA.
Input range is 0, and from 30 to 300.

Monitor screen

NOTICE: Select “STANDARD” for Model L-2100/2130 pump.

4 - 22

4.3.3 Purge Flow Rate

Set a flow rate when executing purge.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

1 EVENT 4 LINK= OTHERS=6

Select CONDITION.

CONDITION SET Z-ADJ=1 MOTOR-CTRL=2

2 PURGE=3, CALIBRATION=4

Select PURGE.

PURGE FLOW: FLOW [--]
<0.001-2.499> 2.499

Enter a purge flow rate.

Monitor screen

Pressing the Purge key in PUMP ON status activates purging at the set flow rate. Pressing
the Purge key again during purge will reset the purge function. During purge, the upper
pressure limit is set at 2.9 MPa (30 kgf/cm2) and the lower pressure limit at
0 MPa (0 kgf/cm2).

4 - 23

4.3.4 Flow Calibration

Set a parameter of flow rate calibration.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

1 EVENT 4 LINK= OTHERS=6

Select CONDITION.

CONDITION SET Z-ADJ=1 MOTOR-CTRL=2

4 PURGE=3, CALIBRATION=4

Select CALIBRATION.

CALIBRATION FLOW=1PRESS=2

Select FLOW.

FLOW CALIB. 0.97 – 1.03

1.000

Enter a parameter o flow rate calibration

Monitor screen

.

4 - 24

4.3.5 Pressure Calibration

Set a parameter of pressure calibration.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

EVENT 4 LINK=5 OTHERS=6

Select CONDITION.

CONDITION SET Z-ADJ=1 MOTOR-CTRL=2

4 PURGE=3, CALIBRATION=4

Select CALIBRATION.

CALIBRATION FLOW=1PRESS=2

2

Select PRESS.

FLOW CALIB. 0.900 – 1.100

1.000

Enter a parameter of pressure calibration

Monitor screen

.

4 - 25

4.3.6 Pressure Unit

Pump pressure unit is selectable from bar, psi, kgf/cm2 and MPa.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

2 EVENT 4 LINK= OTHERS=6

Select UNIT.

PRESS UNIT: <bar=1, psi=2, kgf/cm2=3, [--]

<1-4> 1 MPa=4>

Select pressure unit.

Monitor screen

Pressure indication unit is set to selected one.

The set pressure unit is retained in memory even after turning off power supply. The
relationship among selectable pressure units is listed below.

bar psi kgf/cm

2

MPa

10.0 145 10.2 1.00

4 - 26

4.3.7 Column Flushing

Automatic pump stop and/or column flushing after completion of analysis is settable.
By setting for column flushing, the flushing will be done automatically after the end of
analysis. When a salt solution has been used for analysis, for example, elution of the salt
component can be prevented by flushing the column with water.
An example is given where the column is flushed for 30 minutes with 100% liquid C 15
minutes after the end of analysis.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

4 EVENT 4 LINK= OTHERS=6

Select FLUSH.

PUMP AUTO STOP: YES=1, NO=0 [--]

1

Select YES (PUMP AUTO STOP).

COLUMN FLUSH: YES=1, NO=0 [--]

0

[YES]

SET DELAY TIME: TIME (min) [--]
<1.0-600.0> 600.0

Input the time point of pump stop after completion of
analysis.

Monitor screen

4 - 27

FLUSH MODE: PARAM.=1, PROG.(NO.9)=2 [--]
1

Enter “1” for column flushing under constant conditions, or “2” for column flushing
using a gradient.

[PARAM]

SET DELAY TIME: TIME (min) [--]
<1.0-600.0> 600.0

Input the time period from the end of analysis to the
start of column flushing.

COLUMN FLUSH: FLOW [--]

1.000

Input column flushing conditions (flow rate).

FLUSHING TIME: TIME (min) [--]
<1.0-600.0> 30.0

Column flushing time can be input within 1.0 to 600.0
min.

Monitor screen

[NO]

Monitor screen
When the time for column flushing arrives, the flushing is

executed according to No.9 program.

NOTICE: The program for column flushing is fixed at No. 9.

Upon selecting column flushing, program No. 9 is
automatically set for flushing.
When column flushing is set, program No. 9 is not
usable as a gradient program. But program No. 9 is
usable with a link program.
The pump is stopped automatically when program
No. 9 ends.

4 - 28

Following is the relation between delay time and column flush.

Analysis 1

Analysis 2

Analysis 3

Analysis n

∗

Analysis 1

Analysis 2

Analysis 3

Analysis n

∗

Analysis 1

Analysis 2

Analysis 3

Analysis n

∗

Delay time Column flush

Gradient end

(1) With AUTO STOP at YES

(a) When YES is selected for COLUMN FLUSH

Stabilizing time

“n” indicates number of analyses specified with autosampler, etc.

∗

Stabilizing time is the time from the end of one analysis until the start of the
next analysis.
Column flushing starts when delay time has ended.

(b) When NO is selected for COLUMN FLUSH

Stabilizing time

“n” indicates number of analyses specified with autosampler, etc.

∗

Stabilizing time is the time from the end of one analysis until the start of the
next analysis. The pump stops when delay time has ended.

(2) With AUTO STOP at NO

“n” indicates number of analyses specified with autosampler, etc.

∗

Stabilizing time

The pump remains in liquid delivery status.

Delay time COLUMN FLUSH

Pump stop

Pump stop

Delay time

Pump remains in
liquid delivery status.

4 - 29

4.3.8 Event Signal Code

The event should be executed manually. The event function works upon input of an event
code. It is used for starting an external device, for stopping the pump, for sounding the
buzzer, etc.
Also, if a pressure limiter error occurs, a signal can be output at event code No. 4.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

4 EVENT 4 LINK= OTHERS=6

Select EVENT.

EVENT SET: PRESS LIMIT=1, EVENT CODE=2, [--]

1

[PRESS LIMIT]

PRESS LIMIT: NONE=0, PULSE=1, LEVEL=2 [--]
<0-2> 1 (EVENT NO. 4)

Enter the desired code. If a pressure limit error is
detected, an event signal is output at EVENT No. 4.

Monitor screen

SET EVENT CODE: [--]
<0-92>

Input event code.

Monitor screen

4 - 30

Event codes and related operations are explained below.

Contact

Contact

Signal

marker

Control Item Event Code Remarks

Timer

1
2
3
4

Action

10
20
30
40

opens.

Start
point

turns
off.

11
21
31
41

Start
point

closes.

Signal
turns on.

Open

12
22
32
42

1 s

Close

1st digit:
Timer no.
2nd digit:
Action pattern

Contact closes for 1 sec.

∗

∗

Pump 00 Pump stops
Buzzer 92 Buzzer sounds for 5 sec

4 - 31

4.3.9 Error In Signal

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

6 EVENT 4 LINK= OTHERS=6

Select OTHERS.

OTHERS ERROR IN=1LEAK SENSOR =2

1

Select ERROR IN.

ERROR IN SIGNAL DISABLE=0PUMP OFF=1

1

DISABLE: The pump is not used ERROR signal to stop the unit in e-Line

PUMP OFF: The pump stops the flow when it received the ERROR signal

Monitor screen

in e-Line

4 - 32

4.3.10 Leak Sensor

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

6 EVENT 4 LINK= OTHERS=6

Select OTHERS.

OTHERS ERROR IN=1LEAK SENSOR =2

2

Select LEAK SENSOR.

LEAK SENSOR OFF=0 ON=1

0

Select “ON” for using the leak sensor or “OFF” for avoiding its
use

Monitor screen

4 - 33

4.4 Setting of GLP Functions

Explained here are the confidence functions such as logbook display and resetteng.

4.4.1 Logbook

Logbook can be displayed or reset.
(1) Logbook Display

Logbook can be displayed in the following procedure.

Monitor screen

GLP

GLP: LOGBOOK=1, PRESS M.=2, [--]
1 KEY LOCK=3

Select LOGBOOK

LOG BOOK: REPORT=1, RESET=2, [--]

1

Select REPORT for displaying logbook or RESET for resetting the logbook.

REOPORT

LOGBOOK/REPORT [--]

SOLVENT=99999 1 (2 25 2002)

Total flow after replacement of the pump seal will be
displayed. Pump seal replacement date (month, day,
year) is indicated in parentheses.

ESC

Monitor screen

4 - 34

RESET

LOGBOOK/RESET: [--]
(MM, DD, YYYY) 2 25 2002

Logbook is reset.

NOTICE: Input the date of pump seal replacement.

Input is allowed in the order of month (M),
day (D) and year (Y).
Upon resetting, total flow value is reduced to
0 and the integration of flow starts. Unless
the ENTER key is pressed after entry of
all date values, resetting does not become
effective.
Even when the year is the same as the
displayed one, you should enter all values
and then press the ENTER key.

Monitor screen

4 - 35

4.4.2 Pressure Monitor

The number of double-speed section errors in each measurement can be indicated.
A double-speed section error indicates that the pressure did not return to the reference level
(previous pressure) even after double-speed control (an increase of pump motor speed) was
effected to compensate for a pressure drop. This may be caused by formation of air
bubbles in the flow path.

Monitor screen

GLP`

GLP: LOGBOOK=1, PRESSURE M.=2, [--]
2 KEY LOCK=

Select PRESSURE M.

PRESSURE MONITOR [--]

9999 TIMES

Display the number of double-speed section errors.

ESC

Monitor screen

Resetting occurs on the following occasions.

On input of [PUMP ON] or [PUMP OFF]

•

On activation of contact

•

On start of gradient elution

•

On reception of start signal from integrator in D-line configuration

•

On external start of pump

•

On input of [PURGE ON] or [PURGE OFF]

•

4 - 36

4.4.3 Key Lock

Keyboard keys can be locked.

Monitor screen

GLP

GLP: LOGBOOK=1, PRESSURE M.=2, [--]
3 KEY LOCK=3

Select KEY LOCK.

KEY LOCK: YES=1, NO=0

1

Locks the keys on the operation panel in order to prevent undesired key entries.

Monitor screen

(LOCK OFF)

ESC

Key lock can be released by the [ ESCAPE (LOCK OFF) ] key.

Monitor screen

3.8.3

4 - 37

1

5. MAINTENANCE

5.1 Checking the Performance and Specifications

Performance checking method is explained here.

5.1.1 Items to be

Prepared for

Performance

Check

The following items are required for performance check.

(1) Component Units of Liquid Chromatography System

Pump (Hitachi L-2100/2130 or equivalent)

•

Detector (Hitachi L-2400 or equivalent)

•

Data processing unit

•

Waste liquid bottle

•

: If deaeration of detergent is inadequate, full

NOTE

performance may not be provided. In this
case, deaerate the detergent sufficiently of
connect it through a degasser.

(2) Items for Performance Check

Prepare the following items for performance check.

Load resistance coil

•

A coiled stainless tube with an inner diameter of

0.25 mm and a length of 10 m

60% methanol in about 1000 mL

•

A mixture of methanol 60% and distilled water 40% in
volume. This should be prepared separately.

Distilled water in about 500 mL

•

(3) Performance Check Parts Attached to Model L-2200

Autosampler

Standard sample

•

60% methanol solution of methylparaben
(Methylparaben 6 mg in methanol 60 mL + distilled
water 40 mL)

Sample vial, cap, septum

•

Standard rack

•

5 -

2

(Injection valve)

Waste liquid

5.1 Checking the Performance and Specifications

5.1.2 Preparing the

System for

Operation

(1) Figure 5-1 shows how to connect the units for performance

check. According to this figure, carry out tubing.
Before energizing the system, ensure that the tubing
between the autosampler and pump/detector is properly
connected.

Detector (wavelength 250 nm, time constant 0.1 s)

Load resistance coil
stainless pipe 0.1 mm i.d. × 10 m long(L-2100)
stainless pipe 0.25 mm i.d. × 10 m long(L-2130)

Autosampler

bottle

(2) Connect the e-Line cable among the autosampler, detector

and pump.

(3) Check if the drain tube is properly connected to the waste

liquid bottle. The waste liquid bottle must be at a lower
position than the autosampler.

Pump

Waste liquid
bottle

(Syringe valve)

Eluent Detergent

Fig. 5-1 Flow Path Diagram

5 -

3

5.1.2

(4) Prepare other units to be connected to the autosampler as

described in the respective instruction manuals.

(5) Put the drain tube from the detector into the exclusive

detector waste liquid bottle. If this tube is put into other
waste liquid bottle such as for the Model L-2200
autosampler, baseline fluctuation may be caused due to
unstable back pressure.

(6) After confirmation of the above steps, connect the eluent to

the pump, and the detergent to the autosampler. Be sure
to use the eluent and detergent of the same production lot.

(7) Start the pump to fill each flow path in the pump and

autosampler with liquid.

(8) After the eluent has reached the injection valve of

autosampler, turn on the autosampler power supply. If the
injection valve is switched over when no liquid is [INITIAL],
the valve seal may be damaged. To prevent this, be sure
to start the pump earlier.
The autosampler will go through the [INITIAL]ialization
sequence, and then the [INITIAL]ial screen will appear.

5 -

4

5.1.3 Checking the
Flow rate
stability

(11) Set the pump flow rate to 0.2mL/min L-2100 .

Set the pump flow rate to 1.0mL/min L-2130.

(12) Set the detector wavelength to 250 nm. Set the detector

time constant to 0.1 s.

(13) Set the following conditions in the data processing unit.

SAMPLE PERIOD : 100 ms
STOP TIME : 3 min

Check the reproducibility of the injection volume in the following
procedure.

(1) Press the PROG key and set the following conditions.

TIME FLOW EVENT [--]

0.0 1.000 1

Setting can be completed by pressing the ESC key.

(2) Prepare 60% methanol for the eluent and detergent.

Wait until the pump, detector, etc. are warmed up and the
baseline stabilizes.

(3) Place a vial containing at least 1 mL of standard sample in

rack position No. 1 and set the rack in the autosampler.

(5) Press the START key of autosampler to start analysis.

The analysis is carried out 6 times. The operating
conditions during analysis are indicated on the display.
Ensure beforehand that the room temperature will not
change suddenly during analysis.

(6) Data Check

Determine the coefficient of variation (RSD) of peak area
by the following calculations.

n

xi

Mean value

Standard deviation

x

SD

1i∑=

=

n

n

∑

1i

=

=

2

)xxi(

−

1n

−

5 -

5

RSD of variation

x

RSD

SD

(%)100×=

xi: Area value due to each injection,
n: Number of injections

Make sure the RSD value is 0.075% or less. However,
this value represents the reproducibility of not the pump
alone but the entire chromatographic system.
Therefore, the value varies with each stability of the
autosampler and detector, accuracy of the data processing
unit and its processing parameters.

5 -

6

5.2 Periodic Maintenance

column will contaminate

sample is apt to remain

drift, but shortens the

Perform period maintenance and inspection according to Table 5-1. This prevents
problems and ensures that your system meets specifications.

Table 5-1 Period Maintenance and Inspection

Check Location Procedure Remarks

1. Small particles
(dust) are often
present in the
solvents.

Solvent
filter

2. The column is
expensive.

Wash the solvent filter
with an ultrasonic cleaner
every 4 months.
Clean the solvent bottle
every 2 days.
Ensure that you are using
the correct solvent when
refilling the solvent
bottle.

1) Do not stop the pump
just after completion
of an analysis!
The pump must be kept
running for 30
minutes after
completion of an
analysis.

2) Do not introduce a
sample containing
particles!
Ensure that the sample
contains no particles
or impurities.

3) Periodically clean
the column!
Components left in the

it.

Intrusion of particles
into the pump causes
increase and/or unstable
pressure and flow and
composition problems will
occur.

• Part of a complex

in the column.
This not only causes
poor stability or

column life.

• Particles present in

the sample plug the
column filters.
Pressure will increases
abnormally. Impurities
in the sample also
contaminate the column.
Impurities cause
erroneous data.

• Occasionally clean the

column by injecting a
large amount (1 to
3 mL) of solvent.
The injection of the
solvent will dissolve
the sample residues.
Cleaning of the column
insures good separation
and stability.

5 -

7

Check Location Procedure Remarks

of the drain

main component of a

tion

fluctuations, a failure

3. The flow path
filter
valve is easily
contaminated.

Wash the filters with an
ultrasonic cleaner every
4 months.

The filters prevent
wornout fragments of the
pump seal from flowing
into the column. If the
filter is contaminated,
the pressure increases
abnormally.

4. The pump is the

high speed liquid
chromatograph.

1) The pump seal is a
consumable part!
Be sure to replace
the seals
periodically.

2) The high pressure
pump must be kept
clean!
Clean the pump every
month.
For cleaning, just
pass isopropanol
through the pump for
30 minutes.

3) The high pressure
pump does not func
properly when
there are bubbles.
Before exchanging the
solvent bottle or
adding solvent into
the bottle, be sure
to turn off the pump.

• Deterioration of the

seal causes leaks.
Leaks lead to poor
baseline stability and
data reproducibility.
The net result of leaks
is degradation of
reliability in
measurement.

• Contamination of the

check valve, which is
the critical part in a
high pressure pump,
causes pressure

in the pressure rise or
easy adhesion of air
bubbles.

• Air bubbles in the pump

cause pressure to
fluctuate or fail to
rise.

5 -

8

5.4 Troubleshooting

NOTICE:

If the pump should become faulty, contact the dealer

from whom you purchased the pump. Repair must be left
to personnel who have undergone Hitachi High­Technologies Corporation technical training since
potential hazards are involved.

5.4.1 Error Messages and Proper Measures

WARNING

Beware of Electric Shock!

Before removing the pump cover for parts replacement, etc., make
sure to turn OFF the power switch and disconnect the power cord
from the receptacle.

(1) If an abnormality occurs on the Model L-2130 pump, an

error message appears on the LCD (Liquid Crystal Display).
Error displays result from either the detection of a fault
during the self-diagnostics at power up (upon
[INITIAL]ialization), or from a problem which occurs
during operation.

Fig. 5-1 shows error detecting functions and the error
messages.

5 -

9

Operation Function Error message

Power ON [INITIAL]ialization

ROM ERROR
RAM ERROR
EPROM ERROR
MOTOR [INITIAL]IALIZE

Power ON Pump operation

PRESSURE MAX LIMIT ERROR
PRESSURE MIN LIMIT ERROR
EXTERNAL STOP!

Error
generating
condition

NO

Error
generating
condition

NO

YES

ERROR
E-LINE ERROR

YES

Fig. 5-1 Error Generation and Error Messages

5 -

(2) Each unit [INITIAL]ializes immediately after its power is

Upper pressure limit

Lower pressure limit

turned on. If a problem occurs, an error message displays
in the LCD. If a problem develops during an analysis, an
error message will also appear in the LCD. Use the CL
key to erase the error messages. Unless the cause of an
error is eliminated, the same error message will display
again.
For units other than the Model L-2100/2130, check the
corresponding instruction manuals.

Table 5-2 Model L-2130 Error Messages

No.

1 e-LINE ERROR Error in

2 ROM ERROR Contents of ROM are

3 RAM ERROR The result of RAM

4 EPROM ERROR Contents of EPROM

5 PRESSURE MAX LIMIT

6 PRESSURE MIN LIMIT

7 MOTOR

8 EXTERNAL STOP! The pump is stopped

Screen Display Contents of Error Measure

This error can be cleared
temporarily through the CL
key. Reconnection is not
possible until you turn the
power on in both the master
and the slave stations.

Turn the power off and then on
again. If the same error
occurs, notify the service
personnel.

Turn the power off and then on
again. If the same error
occurs, notify the service
personnel.

Turn the power off and then on
again. If the same error
occurs, notify the service
personnel.

Check the flow for leaks or
clogging.

Check the flow for leaks or
clogging.

Turn the power off and then on
again. If the same error
occurs, notify the service
personnel.

To clear the error message,
press the CL key.

ERROR

ERROR

[INITIAL]IALIZE
ERROR

communication via
the e-line

abnormal.

check is abnormal.

are abnormal.

exceeded (∗1)

exceeded
Motor rotation is

abnormal.

by the emergency
stop contact of
E-Line.

7.1

(∗1): PRESSURE MAX LIMIT ERROR also appears when the pressure exceed the

internal pressure limit. The limit is changed automatically related
to the flow rate. The internal pressure limit is 20 MPa for 0 to 15
mL/min, 15 MPa for 15.01 to 25 mL/min, and 12 MPa for 25.01 to 30
mL/min.

5 - 10

5.4.2 Troubleshooting

Set the programming mode

setting of the other

Table 5-3 offers a guideline for the troubleshooting of the pump. Refer to this table
first, if a problem occurs.

Table 5-3 Model L-2100/2130 Troubleshooting

No.

1 Input mode cannot be

set by pressing
PROG key.

2 Pump does not start

when pressing the
PUMP ON/OFF key.

3 Gradient delivery

does not start when
pressing the
START/STOP key.

4 Purging cannot be set

or canceled by
pressing the PURGE
key.

5 After appearance of

the Initialization
screen when the power
switch has been
turned on, the E-Line
connection wait
screen remains on the
screen. The display
does not change over
to the monitor
screen.

Symptom Cause Measure

The monitor screen is
not in the programming
mode.

Flow is set at 0. Set the flow through the

1) Display is other
than the monitor
screen.

2) The programming mode
is not set.

Display is other than
the monitor screen.
Numerical input is
awaited.

1) The E-Line cable is
not connected.

2) In the E-Line
configuration, the
other unit connected
is not energized.

by pressing the
[INITIAL] key.

[PROG] of the MANUAL
function.

1) Call the monitor
screen by pressing
the ESCAPE key.

2) Set the programming
mode by pressing the
[INITIAL] key.

Call the monitor screen
by pressing the ESCAPE
key.

1) Connect the E-Line
cable.

2) Turn on the E-Line

unit.

NOTICE:

The whirring sound of the pump during operation varies

depending on such operating conditions as flow rate
and pressure. Although the whirring sound may become
larger anywhere in the high-pressure range, it is not
a symptom of abnormality.

5 - 11

1

6. PARTS REPLACEMENT

(on discharge

6.1 Consumables and Spare Parts

The following table lists part numbers, names, shapes, specifications, etc. of replacement
parts. These parts are necessary to keep the system in optimum operation condition. Use
this when ordering parts. Normal life indicates the lifetime of the part when supplying
methanol at a rate of 1.0 mL/min and pressure of 19.6 MPa.

Part No.

890-1130 Seal for L-

655-1080 Seal for L-

885-2920 Seal

Name Shape and Specifications Position Used

2100

2130

Pump head 640h

Pump head 640h

Drain valve 1920h

Normal
Life

890-1128 Plunger for

L-2100

890-1125 Plunger for

L-2130

890-1206 Check valve

valve) for L­2100

890-1205 Check valve

(on suction
valve) for L­2100

Pump head —

Pump head —

Pump head
If check valve

doesn't work
properly, clean.
If this doesn't
solve the
problem, replace
the valve.

—

6 -

25 mm

2

810-1005 Check valve

(on discharge

1/16 inch (1.57 mm)

1/16 inch (1.57 mm)

/16 inch (1.57 mm)

880-1345

valve) for L­2130

810-1004 Check valve

(on suction
valve) for L­2130

810-1031 Flow path

filter

810-1128 Packing

Discharge valve

Packing (B)
810-1344

Check valve
810-1325

Packing (A)
810-1345

Check valve
810-1325

Packing (A)

Valve holder

25 mm

Suction valve

Filter
638-1423

Packing
638-1422

Pump head

—

If check valve
doesn't work
properly, clean.
If this doesn't
solve the
problem, replace
the valve.

Drain valve 1920h

Drain valve 1000 L

6.2 Flow Path Tubing Parts

Part No. Name Shape and Specifications Position Used

L329235 SUS push screw

(M6)

11

655-1458 SUS push screw S

9.6

655-1459 SUS push screw M

13.5

M6

No. 10 32 UNF

No. 10 32 UNF

OD SUS pipe

OD SUS pipe,
Rheodyne injector

1
OD SUS pipe,
Rheodyne injector

6 -

3

810-1310 PCTFE push screw

(3.2 ID)

810-1311 PCTFE push screw

(2.2 ID)

13.5

M8

M6

3 mm OD PTFE tube

2 mm OD PTFE tube

810-1312 PCTFE push screw

(2.1 ID)

638-1099 SUS ferrule

(SUS 316)
1/16 inch

L369053 PCTFE ferrule

1/16 inch

F275108 PTFE tube

14

No. 10 32 UNF

10.5

3.9

3.9

0.25 mm ID × 1.57 mm OD ×
10 m

2 mm OD PTFE tube

1/16 inch OD SUS
pipe

1/16 inch PTFE tube

Inlet tube for UV
detector

6 -

4

(cont’d)

alve

Part No. Name Shape and Specifications Position Used

F275144 PTFE tube

0.33 mm ID × 1.57 mm OD ×
10 m

• Outlet tube for

UV detector

• UV detector back

pressure tube for
high pressure
gradient elution

Reaction coil for

•

reaction LC

F275112 PTFE tube

0.5 mm ID × 1.5 mm OD × 10 m • For tubing

connection with
reaction pump

• Inlet/outlet tube

for collecting
cell

F275114 PTFE tube

1.0 mm ID × 2.0 mm OD × 10 m

Connecting tube
between
proportioning v
and pump

638-1140 PTFE tube

634-1976 Stainless steel

pipe

1.5 mm ID × 3.0 mm OD × 10 m

0.25 mm ID × 1.57 mm OD ×
5 m

Solvent suction
tube for pump

• Pipe for high

pressure gradient
elution

• Pipe for between

injector and
column

634-1977 Stainless steel

pipe

638-2185 Stainless steel

pipe

633-1263 Nipple (made of

SUS)

0.5 mm ID × 1.57 mm OD ×
10 m

0.8 mm ID × 1.57 mm OD × 4 m

M6

Inlet/outlet pipe
for dynamic mixer

Internal pipe in
pump, drain pipe

1/16 - 1/16 pipe

M6

connection

885-1602 Nipple (made of

SUS)

No. 10
32 UNF

6 -

28

21.5

No. 10
32 UNF

Same as above

5

(cont’d)

Part No. Name Shape and Specifications Position Used

885-1601 Nipple (made of

SUS)

885-2179 Nipple (made of

Daiflon)

M6

No. 10
32 UNF

No. 10
32 UNF

24

No. 10
32 UNF

21.5

Same as above

Same as above

6 -