Harvard Apparatus CMA 31 User Manual

CMA 31 Linear Microdialysis Probe User’s Manual

TECHNICAL INFORMATION

Membrane

Material Polyethersulfone (PES)

Molecular Cut-Off 55,000 Daltons

Outer Diameter 0.26 mm

Length 10 mm

Probe Shaft

Material Polyimide

Length Inlet Tubing 350 mm

Length Outlet Tubing 100 mm

Total Length (tubing + membrane) 460 mm

Outer Diameter 0.19 mm

Double Tubing Outer Diameter 0.63 mm

Double Tubing Length 12 mm

The probe can be sterilized in its inner package with ethylene oxide.

Cutaneous Implantation, Anesthetized Animal

For CMA 31 Linear Microdialysis Probe: To avoid ultra ltration over the membrane

the probe should be used with a Perfusion Fluid consisting of a Ringer solution
containing 30g Dextran 60/1000mL.

1. Anesthetize the animal. Be sure to keep the animal’s body temperature normal
during the surgical procedure and the study period by using a CMA 450
Temperature Controller.

2. Clean and shave the animal.

3. Mark the skin with a felt tip marker where the introducer needle should go in
and exit, leaving at least an 18 mm window if drug transport is studied. Tunnel
the introducing needle at the appropriate depth required for the experiment.

4. Load the CMA pump with a Perfusion Fluid-lled syringe (either a Luer lock

single use syringe or a glass syringe with a xed needle). Make sure there are

no air bubbles.

5. Connect the inlet Luer lock connector to the single use syringe or cut off the
Luer lock connector from the probe inlet with a sharp cut, and connect it to
the glass syringe needle via a blue Tubing Adapter.

6. Start the pump at 5 µL/min. Conrm that liquid is owing from the outlet of

the probe. Maintain this ushing rate for approximately 2-3 min to remove air

bubbles.

7. Reduce the ow rate to operational rates, usually 1-3 µL/min.

8. Remove the protective tubing from the probe. Slide the probe outlet tubing
through the needle end of the introducer carefully so the membrane sits in the
middle of the introducer. Make sure not to kink the tubing.

9. Check that the ow rate is the same as before the insertion. Hold the probe in
place against the skin while carefully withdrawing the introducer needle.

10. Secure the probe position with surgical tape or surgical glue.

11. Wait at least 30 min for stabilization before starting the sample collection.

WARRANTY

The probes manufactured by CMA Microdialysis are warranted to be free from defects in material and workmanship for a
period of two years from the manufacturing date if stored in the original package.
Claims should be forwarded without delay to CMA Microdialysis or to your local distributor.

CMA Microdialysis AB

Head Ofce, Sweden

Box 2, 171 18, Solna, Sweden • Tel: [phone] • Fax: [phone]

E-mail: [e-mail] • www.microdialysis.com

Harvard Apparatus

84 October Hill Road
Holliston, MA 01746 USA • Tel: [phone] • Fax: [phone]

E-mail: [e-mail] • www.harvardapparatus.com

ORDER INFORMATION Ref No.

CMA 31 Linear Microdialysis Probe, 4/pkg CMA 8010631

Tubing Adapter, 10/pkg CMA 3409500

FEP Tubing 1m, 1/pkg CMA 3409501

FEP Tubing 1m, 10/pkg CMA 8409501

Vial, plastic, 300µL, 1000/pkg CMA 7431100

Caps, plastic, 1000/pkg CMA 7431101

Vial, Glass 300 µL CMA 7431007

Cap/Seal Non-Reclosing, 1000/pkg CMA 7432175