PHD 4400 Hpsi Programmable MA1 70-2200
PHD 4400 Hpsi Remote Programmable MA1 70-2201
PHD 4400
Syringe Pump Series
User's Manual
Publica tion 5406-00 1-R EV-D
EU Directives WEEE and RoHS
To Our Valued Customers:
We are committed to being a good corporate citizen. As part of that commitment,
we strive to maintain an environmentally conscious manufacturing operation. The
European Union (EU) has enacted two Directives, the first on product recycling
(Waste Electrical and Electronic Equipment, WEEE) and the second limiting the use
of certain substances (Restriction on the use of Hazardous Substances, RoHS).
Over time, these Directives will be implemented in the national laws of each EU
Member State.
Once the final national regulations have been put into place, recycling will be offered
for our products which are within the scope of the WEEE Directive. Products falling
under the scope of the WEEE Directive available for sale after August 13, 2005 will
be identified with a “wheelie bin” symbol.
Two Categories of products covered by the WEEE Directive are currently exempt
from the RoHS Directive – Category 8, medical devices (with the exception of
implanted or infected products) and Category 9, monitoring and control instruments.
Most of our products fall into either Category 8 or 9 and are currently exempt from
the RoHS Directive. We will continue to monitor the application of the RoHS
Directive to its products and will comply with any changes as they apply.
• Do Not Dispose Product with Municipal Waste
• Special Collection/Disposal Required
WEEE/RoHS Compliance Statement
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User 's Manual
Publica tion 5406-00 1-R EV-D
Table of Contents
SUBJECT PAGE NO.
General Information:
Manual Description ..............................................4
Warranty ..............................................................4
Repairs ................................................................4
Serial Numbers ....................................................4
Calibrations ..........................................................4
General Safety Summary ........................................5
Technical Specifications..........................................6
Theory of Operation ................................................7
Features:
Pressure and Speed ............................................8
Built-in Syringe Table and Custom Syringes ......8
Infusion and Refill Rates......................................8
Target Volume ......................................................8
Auto Fill ................................................................8
Modes of Operation ............................................8
External Connections ..........................................9
Nonvolatile Memory ............................................9
Stall Detection ......................................................9
Visual/Audible Alarm ............................................9
Power Up Options................................................9
Program Storage..................................................9
User Interface:........................................................10
Description of Keys ............................................11
Entering Data ....................................................12
Special Features Table ......................................12
Initial Setup ............................................................14
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User 's Manual
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Table of Contents
Operation:
Syringe Loading ................................................15
Running the Pump ............................................16
Diameter ............................................................16
Infuse Rate ........................................................16
Refill Rate ..........................................................16
Target Volume ....................................................17
Auto Fill ..............................................................17
Selecting the Run Mode ....................................17
Program Mode:
Program Description ..........................................19
Entering a Program............................................19
Sequence Operation..........................................20
Program Printout................................................23
Program Run Time Error Messages..................23
External Control and Interfaces:
RS-232C Devices ..............................................24
Configuring Pump for RS-232C Devices ..........24
User I/O Devices................................................24
Programming Tutorial:
Multiple Infusion Example..................................26
Ramping Up Infusion Rate Example ................27
Multiple Dispensing Example ............................28
Periodic Dispense Loop Example......................29
Combination of Infusion and Withdraw
Profiles Example ................................................30
Use of Events ....................................................31
Pump Chain Commands:
Model 22 Protocol..............................................33
Model 44 Protocol..............................................34
SUBJECT PAGE NO.
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Table of Contents
Appendices:
A Table of Syringe Diameters ........................45
B Stainless Steel Syringe ..............................46
C Nominal Min/Max Flow Rates ....................48
D Custom Applications ..................................48
E Pressure and Force Specifications ............49
F PHD 4400 Hpsi to PC Connection ............49
G RS-232C Specifications..............................50
H Symphony ..................................................50
I User I/O Connector Specifications ............51
J Accessories and Spare Parts ....................51
K Maintenance and Troubleshooting ............52
L 'Auto-Fill' Valves..........................................53
Figure 1 Programmable Model Keypad ..............11
Figure 2 Rear Panel and Serial Number Label ..14
Figure 3 Fuse Replacement................................15
Figure 4 Syringe Loading ....................................16
Figure 5 Rear Panel External Connections ........24
Figure 6 Multiple Infusions ..................................26
Figure 7 Ramping Up Infusion Rate....................27
Figure 8 Multiple Dispensing ..............................28
Figure 9 Periodic Dispense Loop........................29
Figure 10 Comb. Infusion & Withdraw Profiles ....30
Figure 11 Use of Events........................................31
Figure 12 Use of TTL Signal ................................32
Figure 13 PHD 4400 Hpsi to PC Connection ......49
Figure 14 RS-232C Specifications ........................50
Figure 15 User I/O Connector Specifications ......51
Figure 16 'Auto-Fill' Valves ....................................53
FIGURE PAGE NO.
SUBJECT PAGE NO.
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User 's Manual
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CCAAUUTTIIOONN:: NNoott ffoorr cclliinniiccaall uussee oonn hhuummaann ppaattiieennttss..
Warranty and Repair Information
MMAANNUUAALL DDEESSCCRRIIPPTTIIOONN
This manual is designed to provide all operational and program information required to
operate and maintain the PHD 4400 Hpsi series pumps. The functions and features are
described in the Technical Specifications section.
WWAARRRRAANNTTYY
Harvard Apparatus warranties this instrument for a period of two years from date of purchase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be
defective as to workmanship or materials. This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends
only to the original consumer purchaser.
IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR
CONSEQUENTIAL DAMAGES. Some states do not allow the exclusion or limitation of
incidental or consequential damages so the above limitation or exclusion may not apply to
you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS
FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this
limitation on an implied warranty, so the above limitation may not apply to you.
If a defect arises within the two-year warranty period, promptly contact Harvard
Apparatus, 84 October Hill Road, Holliston, Massachusetts 01746 using our toll
free number 1–800–272–2775, or outside the U.S. call 508-893-8999. Email Address is bioscience@harvardapparatus.com. Goods will not be accepted for return unless an RMA
(returned materials authorization) number has been issued by our customer service
department. The customer is responsible for shipping charges. Please allow a reasonable
period of time for completion of repairs or replacement. If the unit is replaced, the replacement unit is covered only for the remainder of the original warranty period dating from the
purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary from
state to state.
RREEPPAAIIRR FFAACCIILLIITTIIEESS AANNDD PPAARRTTSS
Harvard Apparatus stocks replacement and repair parts. When ordering, please describe
parts as completely as possible, preferably using a part number obtained from our
Customer Service department. If practical, enclose a sample part or sketch. We offer a complete reconditioning service.
SSEERRIIAALL NNUUMMBBEERRSS
All inquiries concerning our product should refer to the serial number of the unit, located
on the rear panel (See Figure 2).
CCAALLIIBBRRAATTIIOONNSS
All electrical apparatus is calibrated at rated voltage and frequency. While the flow and volume will stay calibrated, the peak pressure may vary.
CCAAUUTTIIOONN:: RREEFFEERR TTOO SSEECCTTIIOONN 77,, IINNIITTIIAALL SSEETTUUPP BBEEFFOORREE
PPLLUUGGGGIINNGG IINN PPHHDD 44440000 HHppssii PPUUMMPP..
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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General Safety Summary
Please read the following safety precautions to ensure proper use of your syringe
pump. To avoid potential hazards and product damage, use this product only as
instructed in this manual.
To Prevent Hazard or Injury:
UUssee PPrrooppeerr LLiinnee CCoorrdd
Use only the specified line cord for this product and make sure line cord is certified
for country of use.
GGrroouunndd tthhee PPrroodduucctt
This product is grounded through the grounding conductor of the power cord. To
avoid electric shock, the grounding conductor must be connected to earth ground.
Before making any connections to the input or output terminals of the product, ensure
that the product is properly grounded.
MMaakkee PPrrooppeerr CCoonnnneeccttiioonnss
Make sure all connections are made properly and securely. Any signal wire connections to the unit must be no longer than 3 meters.
OObbsseerrvvee aallll TTeerrmmiinnaall RRaattiinnggss
Review the operating manual to learn the ratings on all connections.
UUssee PPrrooppeerr FFuussee
Use only specified fuses with product.
AAvvooiidd EExxppoosseedd CCiirrccuuiittrryy
Do not touch any electronic circuitry inside of the product.
DDoo NNoott OOppeerraattee wwiitthh SSuussppeecctteedd FFaaiilluurreess
If damage is suspected on or to the product do not operate the product. Contact qualified service personnel to perform inspection.
PPllaaccee PPrroodduucctt iinn PPrrooppeerr EEnnvviirroonnmmeenntt
Review the operating manual for guidelines for proper operating environments.
OObbsseerrvvee aallll WWaarrnniinngg LLaabbeellss oonn PPrroodduucctt
Read all labels on product to ensure proper usage.
Protective Ground
Terminal
CAUTION
Refer to Manual
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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Specifications
Specifications
Accuracy ±0.35%
Reproducibility ±0.05%
Syringes (Min. / Max.) 0.5 µ l/140 ml
Syringe Diameter 50 mm
Flow Rate:
Minimum 0.0001 µ l/hr (with 0.5 µ l syringe)
Maximum 220.82 ml/min (with 140 ml syringe)
374.39 ml/min (with 50 mm diameter syringe)
Calibration Automatic
Display 2-line, 40-character fluorescent
Memory Nonvolatile (stores all settings)
Interface RS-232C multiplexed dual bidirectional ports
Connectors:
RS-232C RJ11 4-conductor telephone plug
TTL 9-pin D-SUB connector
Linear Force (Max.) 200 lbs
Drive:
Motor 1.8˚ stepper
Control Microprocessor (from 1/2 to 1/32 microstepping)
Step/Revolution from 800 to 12,800
Step Rate:
Minimum 27.3 sec/step
Maximum 416.7 µ sec/step
Pusher Travel Rate:
Minimum 0.18 µ m/min
Maximum 190.676 mm/min
Timing Belt Drive 2:1
Lead Screw Pitch 24 threads/in
Power 100-240 VAC, 50/60 Hz, 75 W, 0.75 A fuse
Dimensions, H x W x D 17 x 23 x 29 cm (6.7 x 9.0 x 11.4 in)
Weight 6.4 kg (14 lbs)
Cable Length (max.) 9.1 m (30 ft) for Remote Model only
15 m (50 ft) for RS-232C
9.1 m (30 ft) for User I/O
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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Theory of Operation
The PHD 4400 Hpsi series employs a microcontroller which controls a small step
angle stepping motor that drives a lead screw and Pusher Block. Microstepping techniques are employed to further reduce the step angle, eliminating flow pulsation. A
keypad is used for entry of operating data to the pump. Data can also be entered via
an RS-232C connector located on the rear panel. The microcontroller, using the internal Syringe Look Up Table, calculates the cross-sectional area of the syringe selected
and calibrates the flow rate and volume accumulation. The numerous features of the
PHD 4400 Hpsi result from the use of microprocessor technology.
The PHD 4400 Hpsi Programmable model provides full programmability along with
Infuse/Withdraw capability. Use of the REFILL RATE key, INFUSE/REFILL key, PROGRAM key, AUTO FILL key, Retaining Brackets and Adjustable Stops are all described in
detail in Section 8 of this manual.
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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Features
PPrreessssuurree aanndd SSppeeeedd
PHD 4400 Hpsi can deliver up to 220.82 ml/minute with a single 140 ml syringe.
Maximum pressure is dependent on syringe size.
BBuuiilltt--IInn SSyyrriinnggee TTaabbllee aanndd CCuussttoomm SSyyrriinnggeess
The user may select the syringe to be used from the pump’s built-in syringe table.
Syringes are arranged according to manufacturer and material, and then according to
size. The pump will look up and use the diameter for the syringe you select. (See
Appendix A for a listing of the standard syringes.)
If a syringe is to be used, which is not listed in the syringe table, enter the inside
diameter of the syringe in millimeters.
IInnffuussiioonn aanndd RReeffiillll RRaatteess
Specify independent rates for infusing and refilling. This allows a slow infusion rate
then a fast refill. If Refill Rate is not set, the software defaults to the set Infuse Rate
value.
TTaarrggeett VVoolluummee
Specify the volume that is to be infused or refilled. The pump will run at the rate specified until this volume has been delivered when in the Volume mode.
AAuuttoo FFiillll
Auto Fill automatically activates an externally attached solenoid (refer to Appendix L
for part number) and refills the syringe when it is empty. This permits infusions to be
virtually independent of syringe capacity.
MMooddeess ooff OOppeerraattiioonn
Pump:
Runs continuously in the infuse or refill directions until stopped.
Volume:
Runs until a specified volume has been pumped or refilled.
Program:
Pump operates according to a specified sequence of instructions.
Note: All modes interact with Auto Fill.
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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EExxtteerrnnaall CCoonnnneeccttiioonnss
User I/O
Allows pump operations to be synchronized with external devices or by a person at a
distance from the pump. Connector pins are available to control direction of pump
travel to control an external valve for refilling, and for general use. A simple contact closure to ground or TTL level signals may be used for inputs. (See Section 10 and
Appendix I.)
RS-232C
Multiple pumps can be 'daisy chained' together and remotely controlled from a computer or any device communicating via RS-232C.
A scale can be connected, enabling the pump to infuse by weight instead of by volume. (Section 10).
A printer can be connected to record final volumes or weights whenever the pump
stops. In addition the program entered for the program mode can be listed on a connected printer. Both a scale and a printer may be connected simultaneously. (See
Section 10).
RReemmoottee UUnniitt ((CCaattaalloogg NNoo.. MMAA11--7700--22220011 OOnnllyy))
Allows connection to the remote syringe pump unit. The remote unit may be positioned up to 30 feet away from the control unit.
NNoonnvvoollaattiillee MMeemmoorryy
All operational data entered into the pump from the keypad or from a computer will
be stored, including the program. On power up, the display will blink until the pump
receives its first command and all settings from when it was powered down will be
recalled.
SSttaallll DDeetteeccttiioonn
An optical detector is used to verify expected movement of the motor. If the motor is
prevented from turning due to jamming or excessive back pressure, the pump will
stop and the display will read: PUMP STALLED.
VViissuuaall//AAuuddiibbllee AAllaarrmm
After a power interruption the alarm can be selected to be a silent flashing display or
an audible signal in addition to flashing display. Refer to Section 6 for details on setting
the Alarm Mode.
PPoowweerr--UUpp OOppttiioonnss ((PPuummpp MMooddee OOnnllyy))
Enables the user to select between a standby mode (waiting for a command to begin
running) after power is interrupted or a restart mode that begins running the pump
at the settings that were in process when the power was interrupted. Refer to Section
6 for details on setting the power-up mode.
PPrrooggrraamm SSttoorraaggee
Programmable model can store up to 4 sets of 9 program sequences for later selection.
Features
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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User Interface
The user interface consists of a keypad with a display area The display consists of a 2
line, 40 character alphanumeric vacuum fluorescent display. The display will show one
of three types of messages: Default display, setting display, or an informational message.
The top line of the display is divided into three sections. The first consists of the delivered volume, or time interval remaining in an operation, expressed with 5 digits plus
a decimal point. Next is the units of the previous number expressed as ‘ml’ for milliliters, ‘g’ for grams, if a scale is attached, or ‘time’ for a time interval. Third is the pumping direction, either ‘INFUSE’ or ‘REFILL’, or the current operating state of the pump. A
diamond symbol ◊ in the last character of the display indicates pump chain communication has been received.
Setting displays are used to facilitate entering control information and data into the
pump. Data entry will be discussed in detail later in this section
Informational messages occur at various times to indicate such items as a data setting
out of range, or a detected problem, such as the pump stalling. Pressing any key clears
the message from the display.
The second line of the display is also divided into 3 sections. The first is the Running
Mode (Pump, Vol, Prog). The second section is the AutoFill indication (no message if
Auto Fill is Off, If On, AutoFill ON is displayed) The third section is a pair of directional arrow heads (< or >) indicating that the pump is running and in what direction.
The keys are used for entering control information and data into the pump. These keys
are grouped into 3 sections (see Figure 1): Set keys, Toggle keys, and Data Entry keys.
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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Figure 1. Programmable Model Keypad
PPHHDD 44440000
User Interface: Description of Keys
SSeett KKeeyyss
SET – Allows modification of a data item in this group of keys. To modify a data item, press
the relevant key after pressing the SET key. Pressing Set with an appropriate data entry key
will also allow the selection of special features. Refer to
EEnntteerriinngg DDaattaa
on page 13.
INFUSE RATE – Displays/sets current infuse rate. Scrolls through rate units with each press
of the key when setting. Displays current programmed rate while running in program
mode.
TARGET VOLUME – Displays/sets current volume mode target volume.
PROGRAM – Displays current program number, program sequence and sequence number.
Held down, with a printer attached and the pump stopped, prints a program listing.
DIAMETER – Displays/sets current syringe diameter. When held down during setting,
accesses built-in syringe table.
REFILL RATE – Displays/sets current refill rate. Scrolls through rate units with each press
of the key when setting.
RS-232C – Displays/sets current RS-232C device(s) attached.
AUTO FILL – Turns Auto Fill feature setting on/off. Also, displays/sets syringe refill volume.
TTooggggllee KKeeyyss
in this group, when permitted, successive states of the keys’ function are selected when
the key is pressed.
RUN/STOP – Starts/stops–interrupts running of pump.
INFUSE/REFILL – Changes direction of pusher block travel during operation in Pump and
Volume modes. Pump must be stopped or in Pump Mode to reverse direction of pump.
SELECT MODE – Toggles in an incremental loop through PUMP, VOLUME or PROGRAM
run modes with each press of the key. The current mode is displayed on the 2nd line of the
display.
DDaattaa EEnnttrryy KKeeyyss
1, 2, 3, 4, 5, 6, 7, 8, 9, 0,.– Used to enter numeric data values or access special features.
ENTER – Saves and stores displayed data value in memory when setting a data item.
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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User Interface: Entering Data
SSeett KKeeyyss
Keys with rectangular outlines in this group are used to modify or review settings of
the pumps control data. To review the current setting of a control data item, simply
hold down the relevant key and the data setting will appear in the top line of the display. In the case of the PROGRAM key, if it is held down the data will be sent to the
printer if one is attached.
To modify a data setting, first press then release the green outlined SET key. The display should then read ‘SET WHAT?’. Press the key in the SET key group whose data is
to be modified. The display will display the current setting. Data is entered into the
pump by either entering a numerical value or by scrolling through a menu of choices.
Always press the green outlined ENTER key to terminate and store each data request
by the pump.
If you are to enter a numerical value, the far left of the display will show ‘ENTER’ followed by the units of the number to be entered. Using the numerical keys on the right
side of the keypad (see Figure 1) enter the new data value. Up to five digit numbers
are accepted, including up to four decimal places.
Entering more than five digits will clear the previous five digits entered in the display.
Press the green outlined ENTER key when the desired data value is displayed.
If the far left of the display does not show ‘ENTER’, then a menu of choices is being
displayed. Pressing the relevant key, according to the choices being displayed, selects
successive menu entries. When the desired selection is displayed, press the green outlined ENTER key.
If the data value entered is outside the pump’s operating parameters, the display will
read ‘OUT OF RANGE’. Pressing any key will restore the display with the original data
value. Enter another data value within the pump’s parameters or just press the green
outlined ENTER key to reuse the original data value.
The data value entered can be reviewed as described above. Note: Certain data items
have multiple settings. For these, after the ENTER key is pressed, the display will
prompt you for the additional information. Various rules apply to when, what and how
data can be set at various times. See the relevant section for further details.
SSPPEECCIIAALL FFEEAATTUURREESS AACCCCEESSSS TTAABBLLEE
Pressing the green outlined SET key and then the appropriate numerical
key will allow the setting of several special features. Pressing the appropriate numerical key again will scroll between the options provided. Refer to
the table below. When the desired option is displayed, press the green outlined ENTER key.
RS-232C Protocol SET 1
Alarm Mode SET 2
Power Up Mode SET 3
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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User Interface: Entering Data
Step Key Setting RS-232C Protocol Description
1 SET SET WHAT? Press and release SET key
Pump Mode
21MODEL 44 PROTOCOL Press and release 1 key
Pump Mode Current RS-232C protocol
displayed
31MODEL 22 PROTOCOL Press and release 1 key to
Pump Mode change RS-232C protocol
4
enter XXXXX ml INFUSE Press ENTER to accept +
Pump Mode return to main display
Step Key Setting Alarm Mode Description
1 SET SET WHAT? Press and release SET key
Pump Mode
22ALARM: VISUAL ONLY Press and release 2 key
Pump Mode Current alarm mode
displayed
32ALARM: VISUAL & AUDIBLE Press and release 2 key to
Pump Mode change alarm mode
4
enter XXXXX ml INFUSE Press ENTER to accept and
Pump Mode return to main display
Step Key Setting Power-Up Mode Description
1 SET SET WHAT? Press and release SET key
Pump Mode
23POWER UP: STANDBY Press and release 3 key
Pump Mode Current power-up mode
displayed
33POWER UP: RUNNING Press and release 3 key to
Pump Mode change POWER-UP mode
4
enter XXXXX ml INFUSE Press ENTER to accept and
Pump Mode return to main display
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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1. Read the entire manual to become familiar with all features and functions of the
PHD 4400 Hpsi.
2. The operating voltage range for the PHD 4400 Hpsi is 100 - 240 VAC, 50/60 Hz.
Use only the specified line cord for this product and make sure line cord is certified for country of use.
3. Take this opportunity to fill out and mail the Warranty Card. The Catalog and
Serial numbers are located on the Serial Number label on the rear panel. Refer to
Figure 4.
4. Turn on main power switch (see Figure 2a) located directly above the power
cord receptacle on the rear panel. The two-line display will illuminate indicating
that the power connections are correct. The flashing display indicates that power
has just been applied. Pressing any key will reset the blinking display to constant
illumination .
5. See Section 8 for operating Instructions on next page.
6. Fuse Replacement. Turn off power, remove power cord from power module. Use
straight blade screw driver, pry open access door. Remove fuse holder, remove
fuse(s) from holder. Replace fuse(s) and fuse holder. For continued fire protection, replace fuse only with 250 V fuse of the specified type and rating. (3 AG 0.75
AMP SLO-BLO) (See Figure 3.)
7. Remote Unit Connection (Catalog no. MA1-70-2201 only). Connect the control
unit to the remote pump unit with the cable provided.
Initial Setup
C
Figure 2a. Rear Panel: Power Entry and Serial Number Label
Figure 2b. Remote Unit Connection
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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Because of the wide range of functions that the PHD 4400 Hpsi is capable of performing, certain information about your application must be entered into the pump.
At minimum, the pump needs to know the diameter of your syringe, the infusion rate
and direction of travel.
This is the only information needed to run the pump in the Pump Mode. If not specified, the refill rate will default to the infuse rate. The pump will need additional information to utilize its more advanced features. See Section 6, User Interface, for general
information on data entry.
Operation
CCAAUUTTIIOONN::
UUssee iinn mmaannnneerr nnoott ssppeecciiffiieedd bbyy tthhee mmaannuuffaaccttuurreerr mmaayy iimmppaaiirr tthhee pprrootteecc--
ttiioonn pprroovviiddeedd bbyy tthhee eeqquuiippmmeenntt..
Figure 3. Fuse Replacement
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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1. The syringe holder and pusher block are fitted with movable retaining brackets
which hold firmly the syringe barrel and plunger when refilling. When loading
the syringe into the pump it is necessary to adjust these brackets. The pusher
block is fitted with a mechanism to release the drive nut from the leadscrew so
that the block can be moved freely to facilitate loading the syringe.
2. Loosen the screws on the syringe block and pusher block to free the retaining
brackets (2 & 3).
3. To free the pusher block from the leadscrew, turn the knob on the front of the
block (1) until the knob slips into the slots or front of the machine.
4. The syringe clamp locking screw on the right side of the syringe block (4) should
be loosened and the clamp rotated to the side.
5. Place the syringe barrel on the syringe holder block and move the pusher block
to accommodate the plunger.
6. Make sure the syringe barrel flange and the plunger flange are held by the retaining clamps. Press the retaining brackets firmly against the flanges and tighten the
retaining screws.
7. Rotate the syringe clamp and press down firmly on the syringe barrel. Secure in
place by tightening the locking screw (4). For syringes greater than 50 cc, it may
be necessary to use the ‘V’-shaped clamp provided. Place clamp over the syringe
barrel and secure to the holder block with the two long thumbscrews.
8. If desired, adjustable stops to limit the syringe plunger travel may be purchased
from Harvard Apparatus (see Appendix J for part number). Install the stops on the
two guide rods at the desired locations.
Operation
1
2
4
3
Figure 4. Syringe Loading
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Harva rd Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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Operation
RRuunnnniinngg tthhee PPuummpp
Pressing the RUN/STOP key starts the pump. The pump will operate according to the relevant data entered as interpreted by the selected run mode. Pressing the RUN/STOP key
while the pump is running stops the pump and the right side of the top line of the display
will indicate ‘INTERRUPT’ plus ‘>’ for infusing or ‘<’ for refilling. This indicates that the
pumping operation has been suspended and can be continued. Pressing the RUN/STOP
key again will continue the pumping operation at the point that it was interrupted.
Changing any of the settings, including the pumping direction and the run mode, cancels
the interrupted operation and resets the display. The pump can also be started and stopped
from a remote source.
DDiiaammeetteerr
If the inside diameter of the syringe being used is known, enter the value in millimeters.
Otherwise, access the built-in Syringe Table and select the syringe being used. After a new
diameter is entered, directly or via the built-in Syringe Table, the Infuse Rate and Refill Rate
are set to 0 and the Auto Fill feature is turned off. This is done for reasons of safety. The maximum diameter that can be entered is 50 mm.
To access the built-in Syringe Table, after pressing the SET key then the DIAMETER key, hold
down the DIAMETER key for about one second. Once the Syringe Table has been accessed,
each press of the key will scroll through the manufacturers. Find the manufacturer and
material, if applicable, of your syringe. Press the ENTER key to enter your selection. Now,
using the DIAMETER key again, scroll by pressing the DIAMETER key until the size of your
syringe is displayed, in cc or µL, as indicated on the display. Pressing the ENTER key will
select the size of the syringe and look up and store the diameter. The diameter will be displayed until the ENTER key is released. Thereafter, pressing the DIAMETER key will display
the selected diameter. In addition, the syringe size selected becomes the default Refill
Volume when the Auto Fill feature is turned on. See Appendix A for a listing of the built-in
Syringe Table and their respective diameters.
IInnffuussee RRaattee
The Infuse Rate is the rate of pumping while infusing in the Pump or Volume modes. Also,
the Infuse Rate is used as a starting rate for the program model if one is not specified in the
program, regardless of pumping direction.
When entering the Infuse Rate, the INFUSE RATE key is used to scroll through the allowable units of rate. The allowable units are: ml/mn, ml/hr, µl/mn, µl/hr. While running in the
Pump or Volume modes, the Infuse Rate can be changed. If the new rate is valid, it will take
effect when the ENTER key is pressed. The minimum and maximum rates permitted vary
depending on the diameter of the syringe. If an ‘OUT OF RANGE’ message is displayed
when entering a rate, try using a different syringe for your application.
RReeffiillll RRaattee
The Refill Rate is the rate of pumping while refilling in the Pump or Volume Modes or during Auto Fill. If the Refill Rate hasn’t been set (rate is 0), the Refill Rate will default to the
Infuse Rate. When entering the Refill Rate, the REFILL RATE key is used to scroll through
the allowable units of rate. The allowable units are: ml/mn, ml/hr, µl/mn, µl/hr. While running in the Pump or Volume modes, the Refill Rate can be changed. If the new rate is valid,
it will take effect when the ENTER key is pressed. The minimum and maximum rates permitted vary depending on the diameter of the syringe. If an ‘OUT OF RANGE’ message is
displayed when entering a rate, try using a different syringe for your application.
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Operation
TTaarrggeett VVoolluummee
The Target Volume is the volume that you desire to deliver at the set Infuse or Refill Rate.
The pump must be set for this Volume mode for this feature to work. When entering the
Target Volume, enter the volume desired in mls (milliliters) and press the ENTER key.
AAuuttoo FFiillll
Use AUTO FILL key to toggle between Auto Fill ‘ON’ and ‘OFF’. If Auto Fill is set to ‘ON’, the
pump will next request the volume of the syringe in milliliters. The volume of the syringe
is used as the refill volume of the syringe. This volume can be set to a volume smaller than
the syringe if desired.
When set to ‘ON’, the syringe is assumed to be empty. Auto Fill continuously monitors the
volume of the syringe according to the volume pumped. When the pump determines that
the syringe is empty, the operation in progress is suspended and Auto Fill is activated. The
pumping direction is then reversed and the pump runs at the refill rate. During the Auto
Fill operation, the display will indicate the volume of liquid in the syringe.
When the volume in the syringe reaches the set refill volume, Auto Fill will stop, and the
previous operation of the pump will resume. Auto Fill continues to monitor the volume of
the syringe. TTL direction output is toggled ‘ON’ during refill. Refill Rate defaults to Infuse
Rate if not set.
Note: Auto Fill will only activate while infusing, (i.e., if the pump direction is set to Refill,
the pump will not stop when the syringe is full.) Also, if the syringe plunger is manually
moved, the pump will lose track of the true syringe volume.
SSeelleeccttiinngg tthhee RRuunn MMooddee
After entering any necessary operating data into the pump, select the pumping mode that
will be used when the pump is operated. Pressing the SELECT MODE key advances the
second line of the display to indicate the next run mode. Advance the run mode to the
desired mode, either Pump Mode, Volume Mode or Program Mode until it is displayed.
PPuummpp MMooddee
The pump will continuously pump, infusing or refilling, until stopped. While running, the
Infuse and Refill Rates can be changed. The new rate, for the relevant pumping direction,
takes effect when the ENTER key is pressed. Also, the pumping direction can be changed
by pressing the INFUSE/REFILL key.
VVoolluummee MMooddee
The pump will run, infusing or refilling, until a specified target volume is pumped or
refilled. The TARGET VOLUME key is used to enter the Volume Mode target volume. Used
in conjunction with Auto Fill, the target volume can be greater than the volume of the
syringe. While running, the Target Volume, Infuse and Refill Rates can be changed. The new
rate, for the relevant pumping direction, takes effect when the ENTER key is pressed. Target
Volume can be used to enter a Target weight in grams if a scale is attached via RS-232C.
PPrrooggrraamm MMooddee
In the Program Mode the pump can make complex dispenses including changes in rate
and target volume. These complex dispenses are easily programmed from the keypad and
are detailed in the Program Mode and the Programming Tutorial sections.
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Program Mode
PPrrooggrraamm DDeessccrriippttiioonn
A program is made up of a set of sequences. Each sequence being a set of operating
instructions for the pump to follow. When the pump is started in the PROGRAM run
mode, the pump will start at sequence 1 and execute the operating instructions in that
sequence. When the pump has completed the instructions for a sequence, it will go to
the next, or specified, sequence and execute the instructions in that sequence. The
pump continues this process until it either has reached a ‘STOP’ operation, the pump
is manually or remotely stopped, or the last sequence has been completed. 4 programs
with up to 9 sequences each may be entered.
A sequence consists of a sequence number, indicating the order of the sequence; a
mode, indicating what operation the sequence will be performing; and the actual data
for the operation, such as rates and volumes. The necessary data specified for each
sequence will depend on the strategy used.
One of two strategies may be chosen for a sequence’s target. Strategy 1 (Target
Volume) pumps until a target volume is reached, while Strategy 2 (Time Interval)
pumps until a target time interval has lapsed. When Strategy 1 is used, enter a time
interval of 0:00:00, then you will be prompted for the target volume. See the
Programming Tutorial for example programs.
EEnntteerriinngg aa PPrrooggrraamm
It is advisable to plan out your program prior to entering the program into the pump.
Press the SET key then the PROGRAM key to begin entering a program.
The following is a list of possible data that can be requested when entering a program
and instructions on entering the data.
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Program Mode
SSeeqquueennccee ooff OOppeerraattiioonn
Use the PROGRAM key to select the sequence’s operation. Operations that can be
selected are: Profile, Increment (Incr), Decrement (Decr), Dispense, Event, Go To,
Pause, Pump, TTL Out, Restart, Stop. When the required operation is displayed press the
ENTER key. Additional information may be requested.
Rate
Enter the rate, using the INFUSE RATE key to change units. Note: If the rate entered is
invalid, an error message will not be given at the immediate time of entry. An ‘OUT OF
RANGE’ error message will be given during the running of the program.
Delta Rate
Enter the rate of Increment or Decrement desired. The units of the rate cannot be specified. Units will be the same as the units of the current pumping rate at the time the
sequence is executed.
Target Volume
Enter the required delivered target volume of the sequence. For increment and decrement sequences, the target volume is an incremental target. An incremental target is
added to the delivered volume at the start of the sequence.
Time Interval
Enter the time duration of the sequence in the form: ‘hours : minutes : seconds’. If
sequence Strategy 1 (Target Volume) is used, enter 0:00:00 for the time target. The maximum time interval is 9:99:99.
Number of Repetitions
Enter the number of times the sequence is to be repeated. The repetition number can
be from 1 to 99,999.
Pumping Direction
Each sequence that specifies a pumping operation, also specifies a pumping direction.
Use the INFUSE/REFILL key to change the pumping direction (< or >).
Pin Level
Select either HI or LOW for the logic level of the programmable output pin 4. Use the
PROGRAM key to change the setting.
Go to sequence number
Enter the destination sequence to continue operation of the program. Valid sequence
numbers are 1 to 10.
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Profile
Runs at specified flow rate until target volume is pumped or a time interval has
elapsed. Travel direction is as specified.
Data Specified:
Strategy 1: Flow rate
(Volume) Time Interval = 00:00:00
Target volume
Pumping direction
Strategy 2: Flow rate
(Time) Time interval
Pumping direction
Incr
Increments current rate by specified value and pumps until the target volume is
pumped or a time interval has elapsed. Units of rate will be that of the current rate of
the pump or the infusion rate’s units, if first sequence.
Sequence is repeated the specified number of times. Travel direction is as specified.
Data Specified:
Strategy 1: Delta flow rate
(Volume) Time Interval = 00:00:00
Volume increment
Number of repetitions
Pumping direction
Strategy 2: Delta flow rate
(Time) Time interval
Number of repetitions
Pumping direction
Decr
Same as INCR except rate is decremented.
Program Mode
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Program Mode
Dispense
Repeatedly dispense specified volume. Runs at specified flow rate until a volume is
pumped or a time interval has elapsed, then pump will stop. If no time interval was
specified (Strategy 1), the display will show ‘TRIGGER’ and the next dispense will
begin after an external or keyboard run command. Otherwise, the sequence will pause
for specified time interval. Sequence is repeated the specified number of times. Travel
direction is as specified.
Data Specified:
Strategy 1: Flow rate
(Volume) Time Interval 00:00:00
Target volume
Number of repetitions
Pumping direction
Strategy 2: Flow rate
(Time) Target volume
Time interval
Number of repetitions
Pumping direction
Event
Program Events – A program event is an external event defined as a high to low transition on TTL pin-9. Within a program, a one time event trigger can be set which watches for and acts upon the external event. The triggered event causes an immediate continuation of the program at the specified sequence and the operation of the pump will
be according to this sequence.
Data Specified:
Go To sequence number
Go To
Causes the program to immediately continue operation at the sequence specified.
Data Specified:
Go To sequence number
Pause
Pump stops for specified time then continues with next programmed sequence.
Current program rate set to 0, with no change in units.
Data Specified:
Time interval
Pump
Runs the pump continuously at the specified flow rate without any pumping target.
This mode can provide a background flow rate while waiting for an external event to
trigger a new sequence specified by the EVENT operation.
Data Specified:
Flow rate
Pumping direction
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Program Mode
TTL Out
Programmable TTL Pin. TTL output Pin 4 of the user I/O connector can be set to a
HIGH or LOW level from within a program.
Data Specified:
TTL pin level
Restart
Immediately restart program from the first sequence.
Data Specified:
None
Stop
Stops pump and the program terminates.
Data Specified:
None
PPrrooggrraamm PPrriinnttoouutt
If a printer is attached and the pump is stopped, a program listing can be obtained by
pressing the PROGRAM key for about one second. ‘PRINTING PROGRAM’ will be displayed while data is being sent.
PPrrooggrraamm RRuunnttiimmee EErrrroorr MMeessssaaggeess
If while running a program an operation is requested that cannot be performed, the
pump will stop and an error message will be displayed. Error messages will be displayed with the following format:
Program N SEQ n: message
Where ‘N’ is the Program number and ‘n’ is the sequence number when an error was
detected, and ‘message’ is the indicated error as follows:
INFINITE LOOP
A GO TO sequence cannot specify the current sequence.
INVALID GO TO
The target of the GO TO specified an invalid sequence number.
RATE UNDERFLOW
A decrement sequence decremented a rate to less than or equal to 0.
RATE OVERFLOW
An increment sequence caused an arithmetic overflow.
OUT OF RANGE
Specified or calculated rate is beyond the pumps capabilities with the specified
syringe.
VOL TGT ERROR
A sequence with a volume target cannot follow a sequence with a time target, unless
the volume delivered is zero or the pump is stopped at the start of the sequence entering it, select Program Mode using the SELECT MODE key and press the RUN/STOP key.
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External Control Interfaces
RRSS--223322CC DDeevviicceess
0.5
Remote Unit Connector (MA1-70-21xx, MA1-71-21xx
and MA1-70-2023 only)
Figure 5. Rear Panel External Connections
External devices that can be attached to the PHD 4400 Hpsi are categorized into either
RS-232C devices or user I/O devices. Pump Chains, Scales and Printers are RS-232C
devices, all other devices are user I/O devices. See the Appendix G for specification
details on attaching RS-232C devices and Appendix I for user I/O specifications.
There are two telephone jack type connectors on the back of the unit (See Figure 5).
These are the RS-232C ports. Looking at the back of the pump, the connector on the
right is IN and the left is OUT. Attach the RS-232C connectors in the appropriate port
according to the following chart:
Device
Port
Pump Chain computer side IN
Pump Chain pump side OUT
Scale IN
Printer OUT
CCoonnffiigguurriinngg tthhee PPuummpp ffoorr RRSS--223322CC DDeevviicceess
Press the SET key and then press 1 to select either Model 22 or Model 44 Protocol
(Refer to Section 12, Pump Chain Commands). Press the SET key, then use the RS-232C
key to scroll through the menu of allowable RS-232C configurations. Possible configurations are:
Pump Chain
Scale
Printer
Scale & Printer
After entering the RS-232C configuration, additional information may be requested:
Pump Chain
Enter the 2-digit address assigned to the pump. Note: Each pump in the chain needs a
unique address. After entering the address (up to 99), the baud rate will be requested.
Use the RS-232C key to toggle between the supported baud rates: 1200, 2400 , 9600
and 19200. Note: Each pump in the chain must have same baud rate. See the Section
12, Pump Chain Commands for pump chain control information.
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Scale
Use the RS-232C key to toggle between the supported manufacturers: Mettler,
Sartorius and Ohaus. When a scale is attached, the weight will be read from the scale
and used as the delivered volume whenever the pumping direction of the pump is set
to infuse. When refilling, the syringe diameter is used for volume calculations. When
the scale weight is displayed, the units will be grams.
Printer
No additional information requested when entering. With a printer (capable of serial
port communication) attached, the pump will print the delivered volume whenever
the pump stops or the direction of pumping changes, except before and after Auto Fill
of the syringe. If the pump stops due to the pump stalling, an asterisk (*) will be
appended to the volume printed. In addition, the entered pump program can be listed
on the printer by pressing the PROGRAM key for about one second, with the pump
stopped.
UUsseerr II//OO DDeevviicceess
The pump does not need to be configured to attach a user I/O device. Simply plug the
device into the 9-pin connector on the rear of the pump. See Appendix I for wiring
specifications.
Foot Switch or Relay
Used to start and stop the pump. Pressing the foot switch performs the same function
as pressing the RUN/STOP key on the keyboard. The foot switch connector allows
remote or automated operation of the pump.
Timer
Opening the timer input starts the pump. Closing the timer input stops the pump. The
timer input allows for an externally controlled pumping interval.
Pumping Direction
Sets the direction of pumping. Opening the directional input sets the pump to infuse.
Closing the directional input sets the pump to refill. The pumping direction input is
recognized only in the situations that the INFUSE/REFILL key would be recognized,
i.e., when the pump is stopped or running in the Pump Mode.
Valve Control
The valve control output is an indicator of the direction of pump travel. When the output is high, the pump is set to Refill. A low output indicates Infuse.
In the following examples, the diameter is 26.7 mm and the infuse rate is 50 ml/mn.
To run a program after entering it, select Program mode using the SELECT MODE key
and press the RUN/STOP key. The examples on the following pages include: Multiple
Infusions, Ramping Up Infusion Rate, Multiple Dispensing, Periodic Dispense Loop,
Combination Infuse and Withdraw Profile, Use of Events and Use of Signal.
External Control Interfaces
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Programming Tutorial
MMuullttiippllee IInnffuussiioonn EExxaammppllee
The following program will instruct the
pump to infuse according to the above
graph. The program instructs the pump to
infuse 10 ml at 75.000 ml/mn then infuse
another 5 ml at 25 ml/mn then stop, for a
total of three sequences. Since this graph is
Rate vs. Volume, Strategy 1 will be used
when entering the program.
SEQUENCE
#1
#2
75
50
2
5
0
0 5 10 15
R
ATE
(
ml/min)
VOLUME (ml)
SSEEQQUUEENNCCEE 11::
Key Presses Explanation
SET Allows selection of Programming mode
PROGRAM Press until PROFILE selected
ENTER Enters selection
75 Enter rate of 75.000 ml/mn
INFUSE RATE Press until units are ml/mn
ENTER Enters rate
0 ENTER Enter 0 for the time, this indicates Strategy 1
10 ENTER 10 ml is the first target volume
INFUSE/REFILL Toggles direction to infuse
ENTER Enters sequence’s pumping direction
SSEEQQUUEENNCCEE 22::
Key Presses Explanation
PROGRAM Press until PROFILE selected
ENTER Enters selection
25 Enter rate of 25.000 ml/mn
INFUSE RATE Press until units are ml/mn
ENTER Enters rate
0 ENTER Enter 0 for the time, this indicates Strategy 1
5 ENTER 5 ml is the second target volume
INFUSE/REFILL Toggles direction to infuse
ENTER Enter sequence’s pumping direction
SEQUENCE 3: Key Presses Explanation
PROGRAM Press until STOP selected
ENTER Enters selection and ends program entry
Figure 6. Multiple Infusions.
PROG1 SEQ 1: PROFILE
75.000 ml/mn
10.000 ml
INFUSE
PROG1 SEQ 2: PROFILE
PPRROOGGRRAAMM PPRRIINNTTOOUUTT
25.000 ml/mn
5.0000 ml
INFUSE
PROG1 SEQ 3:
STOP
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PPRROOGGRRAAMM PPRRIINNTTOOUUTT
PROG1 SEQ 1: PROFILE
10.000 ml/mn
0:00:01 INTERVAL
INFUSE
PROG1 SEQ 2: INCR
0.1695 INCR
0:00:01 INTERVAL
INFUSE
59 REPEAT
PROG1 SEQ 3: PROFILE
20.000 ml/mn
0:00:10 INTERVAL
INFUSE
PROG1 SEQ 4: STOP
RRaammppiinngg UUpp IInnffuussiioonn RRaattee EExxaammppllee
Figure 7. Ramping Up Infusion Rate
SEQUENCE
#
2
#
1
#3
2
0
1
5
10
5
0
0 10 20 30 40 50 60 70
TIME (seconds)
RATE (ml/min)
In this example, the pump will ramp up from 10 ml/mm to 20 ml/mn over 60 seconds,
then continue to run at 20 ml/min for another 10 seconds. This is a Strategy 2 Program
requiring four sequences:
1. Specify the initial rate as a profile of 10 ml/mn for one second.
2. Specify the ramp up to 20 ml/mn. Since the minimum resolution of an increment
is one second, it will take 59 steps to reach the target rate. Sequence 2 starts at
time 1 second and ends at time 60 seconds, giving it a duration of 59 seconds.
At one second a step, 59 seconds divided by one second per step equals 59 steps.
The increase per step will by 20 ml/mn minus 10 ml/mn, divided by 59 steps or
0.1695 rounded to four decimal places.
3. Continue running at 20 ml/mn for 10 seconds with a profile operation.
4. Stop the pump.
Programming Tutorial
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Programming Tutorial
MMuullttiippllee DDiissppeennssiinngg EExxaammppllee
75
50
2
5
0
0
EXTERNAL TRIGGERS
TIME
SEQUENCE
#1 #2 #3
RATE (ml/min)
Figure 8. Multiple Dispensing
PROG1 SEQ 1: DISPENSE
35.000 ml/mn
15.000 ml
3. REPEAT
INFUSE
PROG1 SEQ 2: DISPENSE
65.000 ml/mn
25.000 ml
2. REPEAT
INFUSE
PROG1 SEQ 3: DISPENSE
45.000 ml/mn
17.000 ml
2. REPEAT
INFUSE
PROG1 SEQ 4: STOP
PPRROOGGRRAAMM PPRRIINNTTOOUUTT
Here, a series of dispenses are programmed. Each dispense is started by a trigger, such
as pressing the RUN/STOP key or pressing an attached foot switch. Seven dispenses
are programmed: three of 15 ml at 35 ml/mn, two of 25 ml at 65 ml/mn, and two of 17
ml at 45 ml/mn. The pump’s display will show ‘TRIGGER’ when it is waiting for a run
trigger and < or > will not be displayed.
This is a Strategy 1 dispense. A time interval of 0 is specified when entering a Strategy
1 dispense. Since the total volume to be dispensed is 129 ml and the syringe volume
is 50 ml, the Auto Fill feature would be very useful with this program.
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Programming Tutorial
PPeerriiooddiicc DDiissppeennssee LLoooopp EExxaammppllee
TIME (min:seconds)
2
5
2
0
15
10
5
0
0
1:30
1:30
1:30
2:30
2:30
2:30
2:30
43:30 5:00 5:00
RATE (ml/min)
Figure 9. Periodic Dispense Loop
PROG1 SEQ 1: DISPENSE
15.000 ml/mn
3.5000 ml
0:01:30 INTERVAL
3. REPEAT
INFUSE
PROG1 SEQ 2: PAUSE
0:43:30 INTERVAL
PROG1 SEQ 3: DISPENSE
25.700 ml/mn
6.7500 ml
0:05:00 INTERVAL
2. REPEAT
INFUSE
PROG1 SEQ 4: DISPENSE
20.000 ml/mn
4.3000 ml
0:02:30 INTERVAL
4. REPEAT
INFUSE
PROG1 SEQ 5: RESTART
PPRROOGGRRAAMM PPRRIINNTTOOUUTT
This is an example of a series of periodic dispenses of varying volumes and intervals. For
this application, Strategy 2 dispenses are used.
Note that between the third and fourth dispenses is a 45 minute interval. Each dispense in
the first sequence is separated by a pause interval of 1:30. Since after the third dispense there
already will be a 1:30 pause, an additional
pause of 43:30 is used to extend the pause to
the desired 45:00. Sequence 5 is a RESTART
command, causing the series of dispenses to be
continuously repeated until the pump is
stopped.
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Programming Tutorial
CCoommbbiinnaattiioonn ooff IInnffuussiioonn aanndd
WWiitthhddrraaww PPrrooffiilleess EExxaammppllee
100
90
80
70
6
0
50
40
30
20
10
0
6050403020100
TIME (seconds)
RATE (ml/min)
SEQUENCE
#2 #3 #4 #5 #6 #7 #8
Figure 10. Combination Infusion and
Withdraw Profiles
Here is an example of a more complex profile program. Each ‘run’ of
the infusion has been determined
to pump 43.155 ml. The first
sequence refills the syringe with
the volume to be infused then the
infusion profile is started, after
which the syringe is refilled and the
infusion is repeated until the pump
is stopped.
PPRROOGGRRAAMM PPRRIINNTTOOUUTT
PROG1 SEQ 1: PROFILE PROG1 SEQ 5: PROFILE
75.000 ml/mn 95.000 ml/mn
43.155 ml 0:00:10 INTERVAL
REFILL INFUSE
PROG1 SEQ 2: PROFILE PROG1 SEQ 6: PROFILE
50.000 ml/mn 30.000 ml/mn
0:00:04 INTERVAL 0:00:05 INTERVAL
INFUSE INFUSE
PROG1 SEQ 3: DECR PROG1 SEQ 7: PROFILE
4.0000 DECR 65.000 ml/mn
0:00:01 INTERVAL 0:00:05 INTERVAL
12. REPEAT INFUSE
INFUSE PROG1 SEQ 8: DECR
PROG1 SEQ 4: INCR 5.0000 DECR
5.0000 DECR 0:00:01 INTERVAL
8.0000 INCR 11. REPEAT
0:00:01 INTERVAL INFUSE
8. REPEAT PROG1 SEQ 9: RESTART
INFUSE
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UUssee ooff EEvveennttss
Figure 11. Use of Events
This is an example of the PHD 4400 Hpsi working interactively with other laboratory
equipment. The pump will continuously pu
mmpp aa
t 300 ml/hr until an external event, a
high to low transition at pin 9, possibly produced by another PHD 4400 Hpsi , causes
the pump to deliver a 15 ml bolus at 75 ml/mn. After delivering 5 ml of the bolus, output pin 4 is set to a logic high for the duration of the bolus after which it is dropped.
This output pin can be attached to the timer input of another pump, such as a Harvard
Pump 22 or 44, to create a precise mixture during the bolus. After the bolus
iiss ccoomm--
pplleetteedd,,
the Pump 22 would be stopped and the PHD 4400 Hpsi would return to deliv-
ering its background rate of 300 ml/hr, waiting for another external event.
Programming Tutorial
PPRROOGGRRAAMM PPRRIINNTTOOUUTT
PROG1 SEQ 1: TTL OUT 5.0000 ml
OFF INFUSE
PROG1 SEQ 2: EVENT PROG1 SEQ 5: TTL OUT
GO TO 4 ON
PROG1 SEQ 3: PUMP PROG1 SEQ 6: PROFILE
300.00 ml/hr 75.000 ml/mn
INFUSE 10.0000 ml
PROG1 SEQ 4: PROFILE INFUSE
75.000 ml/mn PROG1 SEQ 7: RESTART
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Programming Tutorial
Before the pumping flow begins, the pump sends a TTL signal to another piece of equipment for 5 seconds, but first verifies that the TTL line is low by turning it off for 1 second,
then turns it on. The pumping process consists of an alternation of pumping 3 ml’s at
53 ml/mn and 5 ml’s at 75 ml/mn. It uses the TTL output to signal to another device whose
sequence it is executing by lowering the TTL line before sequence 5 and raising it before
sequence 8.
Figure 12. Use of TTL Signal
This is an example of the PHD 4400 Hpsi being operated from a remote location. When
the pump is powered on, the position of the pusher block is unknown and must be homed
to a known position.
At the syringe full position, a limit switch is placed such that it is tripped by the pusher
block when the syringe is full. The limit switch is connected to pins 4 and 9, programmable output and the event input, on the user I/O connector. When the pump receives a start
signal, it first refills the syringe and stops when the limit switch is sensed. The pump then
waits for a start trigger and performs a dispense then refills the syringe and waits again for
the next start trigger.
PROG1 SEQ 1: TTL OUT
OFF
Prog1 SEQ 2: PAUSE
0:00:01 INTERVAL
PROG1SEQ 3: TTL OUT
ON
PROG1 SEQ 4: PAUSE
0:00:01 INTERVAL
PROG1 SEQ 5: TTL OUT
OFF
PROG1SEQ 6: PROFILE
PPRROOGGRRAAMM PPRRIINNTTOOUUTT
53.000 ml/mn
3.0000 ml
INFUSE
PROG1 SEQ 7: TTL OUT
ON
PROG1 SEQ 8: PROFILE
75.000 ml/mn
5.0000 ml
INFUSE
PROG1SEQ 9: GO TO 5
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Pump Chain Commands
The Pump Chain RS-232C interface is used to enhance the control applications of the
PHD 4400 Hpsi. This interface allows all control information, including a program, to
be entered into the pump from an external source such as a computer. In addition, this
interface allows up to 100 pumps and, in certain cases, other RS-232C devices to be
controlled from a single RS-232C communication port on a computer.
Assign each pump in the pump chain a unique address from 0 to 99. This address is
used to identify which pump is to receive a command and which pump is responding.
Configure each pump with its assigned address and the baud rate being used (See
Section 10, External Control & Interfaces).
When the control device communicates with the pump, a diamond appears on the far
right of the display indicating that it is receiving RS-232C commands. The diamond
remains on the display until the pump is turned off or SET RS-232C is entered on the
keyboard, indicating a change in the RS-232C configuration.
A pump will not respond to pump chain communication while it is in a setting mode
(entered when user presses the SET key). The pump can still be controlled from the
keyboard while it is in a pump chain. Control data that is changed via RS-232C will be
stored in the pump’s nonvolatile memory.
After each command is received and executed, the pump terminates its responses
with a prompt. A prompt is a string of ASCII characters sent by a pump.
The PHD 4400 Hpsi is capable of using two sets of pump interface commands. This
will allow users of Harvard Apparatus Model 22 and Model 44 pumps to use existing
programs to control the PHD 4400 Hpsi pumps. The two sets of commands are contained on the following pages.
PROG1 SEQ 1: EVENT
GO TO 7
PROG1 SEQ 2: TTL OUT
ON
PROG1 SEQ 3: PAUSE
0:00:01 INTERVAL
PROG1 SEQ 4: TTL OUT
OFF
PROG1 SEQ 5: PAUSE
00:00:01 INTERVAL
PROG1 SEQ 6: PUMP
75.000 ml/mn
PROG1 SEQ 7: DISPENSE
10.000 ml/mn
0.0001 ml
1. REPEAT
REFILL
PROG1 SEQ 8: PUMP
75.000 ml/mn
10.000 ml
INFUSE
PROG1 SEQ 9: RESTART
PPRROOGGRRAAMM PPRRIINNTTOOUUTT
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Pump Chain Commands
Each command sent to the pump chain is a string of ASCII characters, with leading
zero’s on numbers and all spaces optional. Numbers are a maximum of five digits. The
following symbols are used in describing the commands:
PPuummpp CChhaaiinn CCoommmmaannddss -- MMooddeell ‘‘2222’’ PPrroottooccooll
((MMooddeell 4444 PPrroottooccooll oonn ppaaggee 3366))
After each transmission to the Pump terminating with a CR character (ASCII 13), the
pump enters remote mode and responds with the three character sequence:
CR LF prompt
The prompt character indicates the status of the pump as follows:
: When stopped (ASCII 58 decimal)
> When running forward (ASCII 62 decimal)
< When running reverse (ASCII 60 decimal)
* When stalled (ASCII 42 decimal)
Note: With a pump chain, if no address precedes the command transmitted, the pump at address 0 will respond to the command.
CCoommmmaannddss -- RReessppoonnssee:: CCRR LLFF pprroommpptt
RUN Infuse (forward direction)
REV Start (reverse direction) Not accessible on Infusion model
STP Stop
CLV Clear volume accumulator to zero
CLT Clear target volume to zero
MLM number Set rate, units are milliliters per minute
ULM number Set rate, units are microliters per minute
MLH number Set rate, units are milliliters per hour
ULH number Set rate, units are microliters per hour
MMD number Set diameter, units are mm. Rate is set to 0
MLT number Set target infusion volume, units are ml.
Numbers between 0 and 1999 will be accepted by the pump. Leading zeros and trailing decimal point are optional. Any number of digits to the right of the decimal point
may be transmitted. The number received will be rounded to four significant digits if
the leading digit is 1, or three significant digits if the leading digit is 2 to 9.
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Pump Chain Commands
QQuueerriieess -- RReessppoonnssee:: CCRR LLFF VVaalluuee CCRR LLFF PPrroommpptt
QQuueerriieess wwiitthh NNuummeerriicc RReessppoonnssee
DIA Send diameter value, units in mm
RAT Send rate value in current range units
VOL Send current accumulated infused volume, units are ml.
TAR Send target volume, units are ml.
VER Send model and version number
Value format: nnnn.nnn
The transmitted value is an 8 character string with leading zeros converted to SP characters. (ASCII 32) The fifth character is a decimal point (ASCII 46).
QQuueerryy -- RReessppoonnssee:: CCRR LLFF RRaannggee CCRR LLFF PPrroommpptt
QQuueerriieess wwiitthh SSttrriinngg RReessppoonnssee
RNG Send range message
Range is a character string, one of: ML/H ML/M UL/H or UL/M
EErrrroorr RReessppoonnsseess
CR LF ? CR LF prompt Unrecognized command
CR LF O O R CR LF prompt Out of range
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Pump Chain Commands
PPuummpp CChhaaiinn CCoommmmaannddss -- MMooddeell ‘‘4444’’ PPrroottooccooll
Symbol Meaning
[. . .] optional
{. . .} select one
| either/or
f digits 0 – 9 or a decimal point
d digits 0 – 9
<cr> carriage return (ASCII 13)
<lf> line feed (ASCII 10)
<float> f f f f f f
<integer> ddddd
<time> d:dd:dd
<text> any string of ASCII characters
CCoommmmaanndd FFoorrmmaattss aanndd MMeeaanniinnggss::
Command Format Meaning
<cr> Stops all pumps.
All pumps on the pump chain interpret
this as a stop command.
pump address, <cr> Request for prompt
The pump with the indicated
address responds with its prompt
optional pump address, Send a command to a pump.
command, <cr> The pump with the indicated
address executes the command
then responds with its prompt.
The optional pump address, if not
specified, will default to pump address 0.
After each command is received and executed, the pump acknowledges the command
with a prompt. Preceding the prompt may be some additional text responses. The additional text will be one or more lines of ASCII text, each preceded by a line feed and
terminated by a carriage return:
<lf>, <text>, <cr>
A prompt is a string of ASCII characters sent by a pump indicating the pumps address
and its present state:
<lf>, 1 or 2 digit address, prompt character
Prompt Characters Meaning
: Pump stopped
> Pump infusing
< Pump refilling
/ Pause interval (pump stopped)
* Pumping interrupted (pump stopped)
^ Dispense trigger wait (pump stopped)
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PPuummpp CCoommmmaannddss aanndd RReessppoonnsseess
RUN Starts pumping according to the present setting of the pump. If pump is
already pumping, a ‘Not Applicable’ response will be given.
STP Stops pump if it was running. If pump was already stopped, a ‘Not
Applicable’ response will be given.
DEL Request for volume delivered, in ml.
Response is of the following format:
space, space, f f f f f f
CLD Request to zero volume delivered. If the pump was interrupted, it will can-
cel the interrupted condition. If the pump is running, request will not be
accepted and a ‘Not Applicable’ response will be given. Otherwise, no
response is given.
RAT [<float> [<units>]]
Request to set or query infusion rate setting.
Set infusion rate:
RAT rate
Set infusion rate and units:
RAT rate units
Rate is of format: f f f f f f
Units are one of:
Definition
UM µl/mn
UH µl/hr
MM ml/mn
MH ml/hr
If rate is accepted and valid, rate will become the new infusion rate.
If the rate is invalid, an ‘Out Of Range’ response will be given.
Command will not be accepted if the pump is running in the Program
Mode and
a ‘Not Applicable’ response will be given.
Query infusion rate: RAT
Response is of the following format:
space, space, f f f f f f units
Where units is one of the following:
ml/mn
ul/mn
ml/hr
ul/hr
Pump Chain Commands
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Pump Chain Commands
RFR [<float> [<units>]]
Request to set or query refill rate setting.
Set refill rate:
RFR rate
Set refill rate and units:
RFR rate units
Rate is of format: f f f f f f
Units are one of:
Definition
UM µl/mn
UH µl/hr
MM ml/mn
MH ml/hr
If rate is accepted and valid, rate will become the new refill rate.
If the rate is invalid, an ‘Out Of Range’ response will be given.
Command will not be accepted if the pump is running in the
Program Mode and
a ‘Not Applicable’ response will be given.
Query refill rate: RFR
Response is of the following format:
space, space, f f f f f f units
Where units is one of the following:
ml/mn
ul/mn
ml/hr
ul/hr
PGR Request for the rate of pumping set during the running of a program.
Response is of the following format:
space, space, f f f f f f units
Where units is one of the following:
ml/mn
ul/mn
ml/hr
ul/hr
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DIA [<float>]
Request to set or query syringe diameter setting.
Set diameter:
DIA diameter
Diameter is of format: f f f f f f
Units are MM.
INFUSE and REFILL rates will be set to zero and AUTO FILL will
be set to off.
If diameter is accepted and valid, diameter becomes new diameter.
Diameter will not be accepted if the pump is running and a ‘Not
Applicable’ response will be given.
If the diameter is invalid, an ‘Out Of Range’ response will be
given.
Query diameter: DIA
Response is of the following format:
space, space, f f f f f f
Units are MM.
TGT [<float>]
Request to set or query target volume setting.
Set target volume:
TGT volume
Volume is of format: f f f f f f
Units are ML.
If volume is accepted and valid, volume becomes new target volume.
Volume will not be accepted if the pump is running and a ‘Not
Applicable’ response will be given.
If the volume is invalid, an ‘Out Of Range’ response will be given.
Query volume: TGT
Response is of the following format:
space, space, f f f f f f
Units are ML.
Pump Chain Commands
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Pump Chain Commands
MOD [{PMP|VOL|PGM}]
Request to set or query pumping mode
Set: MOD PMP (Puts pump in Pump Mode)
MOD VOL (Puts pump in Volume Mode)
MOD PGM (Puts pump in Program Mode)
Command will not be accepted if the pump is running and a ‘Not
Applicable’ response will be given.
Query: MOD
If mode is PUMP, response will be:
PUMP
If mode is VOLUME, response will be:
VOLUME
If mode is PROGRAM response will be:
PRGRAM
DIR [{INF|REF|REV}]
Request to set or query pumping direction
Set: DIR INF (sets pumping direction to infusion)
DIR REF (sets pumping direction to refill)
DIR REV (reverses current pumping direction)
Command will not be accepted if the pump is running in volume or program modes and a ‘Not Applicable’ response will be given.
Query: DIR
If pump direction is infusion, response will be:
INFUSE
If pump direction is refill, response will be:
REFILL
AF [{ON|OFF}]
Request to set or query auto fill setting
Set: AF ON (turns Auto Fill feature on)
Note: The syringe volume is also needed for auto fill to operate.
(See SYR command)
AF OFF (turns Auto Fill function off)
Command will not be accepted if the pump is running and a ‘Not
Applicable’ response will be given.
Query: AF
If Auto Fill function is ON, response will be: ON
If Auto Fill function if OFF, response will be: OFF
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SYR [<float>]
Request to set or query syringe volume setting for auto fill.
Used in conjunction with Auto Fill feature. (See AF command).
Set syringe volume: SYR volume
Volume is of format: f f f f f f
Units are ML.
If volume is accepted and valid, volume will become the new
syringe Auto Fill volume.
Volume will not be accepted if the pump is running and a ‘Not
Applicable’ response will be given.
If the volume is invalid, an ‘Out Of Range’ response will be given.
Query syringe volume: SYR
Response is of the following format:
space, space, f f f f f f
IN d
Request to read the TTL logic level of the specified pin on the external 9
pin D-SUB connector. Valid pin numbers for input are:
6, 7, 8 and 9
If the pin specified is valid and if the pin level is high, response will be:
ON
If the pin level is low, response will be:
OFF
If the pin specified is invalid:
An ‘Out Of Range’ response will be given
OUT d = <ON|OFF>
Request to set the TTL logic level at the specified pin on the external 9 pin
D-SUB connector.
Valid pin number for output is: 4
If the pin specified is invalid:
An ‘Out Of Range’ response will be given
Example:
Set pin 4 high:
OUT 4 = ON
Set pin 4 low:
OUT 4 = OFF
Pump Chain Commands
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Pump Chain Commands
SEQ [<integer>} [<entry>]
Request to set or query programming sequences. <integer> is sequence
number.
Default is Sequence 1. Command only applicable while pump is stopped.
Valid
sequence numbers, ‘n’, are 1 to 10. ‘n’ defaults to 1 wherever it is optional.
See program
examples.
Query entire program: SEQ
Example response:
SEQ 1: DISPENSE
75.000 ml/mn
43.155 ml
0:00:01 INTERVAL
3 REPEAT
INFUSE
SEQ 2: PROFILE
100.00 ml/mn
150.00 ml
REFILL
SEQ 3: RESTART
Query program sequence [n]: SEQ n
Example response to the command ‘SEQ 2’ with the previous example’s
program:
SEQ 2: PROFILE
100.00 ml/mn
150.00 ml
REFILL
Query program sequence n’s mode: SEQ [n] MOD
Response will be according to the following table:
Response
Description Response Description
STP stop RST restart
PRO profile GOT go to
INC increment EVN event
DEC decrement PMP pump
DIS dispense OUT TTL out
PAS pause
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Pump Chain Commands
Query data item of program sequence n:
Command Description
SEQ [n] RAT Query rate
Response:
f f f f f f units
Where units is one of the following:
ml/mn
ul/mn
ml/hr
ul/hr
SEQ [n] GOT Query go to sequence number
Response: <n>
SEQ [n] TGT Query target volume
Response: <float>
SEQ [n] INT Query time interval
Response: <time>
SEQ [n] RPT Query repetition count
Response: <float>
SEQ [n] OUT Query output pin level setting
Response: <ON/OFF>
SEQ [n] DIR Query pumping direction
Possible responses: INFUSE
REFILL
Set mode of program sequence n:
SEQ [n] MOD mode
Where mode is as follows:
Mode
Description Mode Description
STP stop RST restart
PRO profile EVN event
INC increment GOT go to
DEC decrement OUT set output pin
DIS dispense PMP pump
PAS pause
Set data item of program sequence n:
Set sequence’s rate:
SEQ [<n>] RAT <float> [<units>]
Rate is of format: f f f f f f
Units are one of :
Description
UM µl/mn
UH µl/hr
MM ml/mn
MH ml/hr
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Pump Chain Commands
Set sequence’s go to sequence number
SEC [<n>] GOT <n>
Set sequence’s target volume:
SEQ [<n>] TGT <float>
Set sequence’s time or target:
SEQ [<n>] INT <time>
Set sequence’s repetition number:
SEQ [<n>] RPT <integer>
Set sequence’s pumping direction:
SEQ [<n>] DIR <INF|REF>
Set sequence’s output pin level
SEQ [<n>] OUT <ON|OFF>
VER Request for version of pumps embedded software.
Pump Chain Error Messages
Error messages are in the format:
<lf>, space, space, <message>, <cr>,
Where <message> is one of the following:
? Syntax error in a received command
NA Command not applicable at this time
OOR Control data is out of the operating range of the pump
Note: If assistance is needed in programming pump control via RS-232C, call
Harvard Apparatus Customer Service Support at 1-800-272-2775.
Outside of U.S. call 508-893-8999.
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Appendix A: Syringe Diameters in mm
Stainless Steel
Size Diameter
8 cc 9.525 mm
20 19.130
50 28.600
100 34.900
200 44.755
Becton Dickinson
Plastic
‘Plasti-pak’
Size Diameter
1 cc 4.78 mm
3 8.66
5 12.06
10 14.50
20 19.13
30 21.70
50/60 26.70
Air–Tite
‘All Plastic’
Size Diameter
2.5 cc 9.60 mm
5 12.45
10 15.90
20 20.05
30 22.50
50 29.00
Unimetrics
Series 4000 &
5000
Size Diameter
10 µl 0.460 mm
25 0.729
50 1.031
100 1.460
250 2.300
500 3.260
1000 4.610
Terumo
Size Diameter
3 cc 8.95 mm
5 13.00
10 15.80
20 20.15
30 23.10
60 29.10
Sherwood–Monoject
Plastic
Size Diameter
1 cc 4.65 mm
3 8.94
6 12.70
12 15.90
20 20.40
35 23.80
60 26.60
140 38.40
Popper & Sons, Inc.
‘Perfektum’ Glass
Size Diameter
0.25 cc 3.45 mm
0.5 3.45
1 4.50
2 8.92
3 8.99
5 11.70
10 14.70
20 19.58
30 22.70
50 29.00
100 35.70
Renfac
Size Diameter
2 cc 9.12 mm
5 12.34
10 14.55
20 19.86
30 23.20
50 27.60
SGE Scientific
Glass
Engineering
Size Diameter
25 µl 0.73 mm
50 1.03
100 1.46
250 2.30
500 3.26
1.0 ml 4.61 mm
2.5 7.28
5 10.30
10 14.57
Hamilton–Microliter
Series Gastight
Size Diameter
0.5 µl 0.103 mm
1 0.1457
2 0.206
5 0.3257
10 0.460
25 0.729
50 1.031
100 1.46
250 2.3
500 3.26
1.0 ml 4.61 mm
2.5 7.28
5 10.3
10 14.57
25 23.0
50 32.6
Becton Dickinson
Glass-All Types
Size Diameter
0.5 µl 4.64 mm
1 4.64
2.5 8.66
5 11.86
10 14.34
20 19.13
30 22.70
50 28.60
100 34.90
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Appendix B: Stainless Steel Syringes
HHaarrvvaarrdd HHiigghh PPrreessssuurree SSttaaiinnlleessss
SStteeeell SSyyrriinnggeess
Harvard now offers a completely new
line of Stainless Steel Syringes intended
for high pressure applications with good
resistance to most aggressive liquids.
Wetted parts are #316 stainless steel or
Viton. Syringes are available in 20, 50, 100
and 200 ml sizes with removable replaceable tips. Syringe to tube fittings are available in 1/16, 1/8 and 1/4 inch sizes. A luer
lock end fitting is also available. All tips
are interchangeable with all syringes (20
to 200 ml) in the series.
Both syringe barrel end plungers are
#316 stainless steel. A Viton O-ring seal
between top and end of the barrel
insures against leakage. Syringes are
guaranteed to be leak free for pressures
up to 700 psi.
All syringes are supplied with inside
diameter dimensions for use with
Harvard microprocessor controlled
pumps and rate charts for use with older
‘classic’ pumps. Replacement Viton ORings are available, as are the more chemically resistant Chemraz®O-Rings.
Harvard High
Pressure Stainless Steel Syringes
8 ml
200 ml
100 ml
50 ml
20 ml
Cat No Product
Replacement Parts
MA1-5013-089 Chemraz
®
O-Ring 20 ml
MA1-5013-090 Chemraz®O-Ring 50 ml
MA1-5013-091 Chemraz®O-Ring 100 ml
MA1-5013-092 Chemraz®O-Ring 200 ml
MA1-5013-109 Chemraz®Tip Seal O-Ring, all
sizes
MA1-72-2472 Replacement Viton O-Ring 20
ml, pkg. of 10
MA1-72-2473 Replacement Viton O-Ring 50
ml, pkg. of 10
MA1-72-2474 Replacement Viton O-Ring
100 ml, pkg. of 10
MA1-72-2475 Replacement Viton O-Ring
200 ml, pkg. of 10
MA1-72-2616 Replacement Viton Tip Seal
O-Ring, all sizes, pkg. of 20
MA1-72-2617 Stainless Steel Plunger Button
to Adapt Syringe for Use with
PHD 22/2000 HVP with Triple
S Clamp (Required for 50 ml
and 100 ml syringes only)
Harvard High Pressure Stainless Steel Syringes
Fitting for Tube Diameter Luer Lock
Syringe Size 1/16 inch 1/8 inch 1/4 inch
20 ml MA1-70-2251 MA1-70-2252 MA1-70-2253 MA1-70-2254
50 ml MA1-70-2255 MA1-70-2256 MA1-70-2257 MA1-70-2258
100 ml MA1-70-2259 MA1-70-2260 MA1-70-2261 MA1-70-2262
200 ml MA1-70-2263 MA1-70-2264 MA1-70-2265 MA1-70-2266
Replacement Tips, Furnished with Sealing O-Ring
MA1-70-2247 MA1-70-2248 MA1-70-2249 MA1-70-2250
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Specifications
Volume 8 ml
Dimensions:
Length of Barrel 16.5 cm (6-1/2 in)
Plunger Excursion 11.4 cm (4-1/2 in)
OD 1.6 cm (5/8 in)
ID 0.95 cm (3/8 in)
Max Test Pressure 4000 psi
Working Pressure 1500 psi
Cat No Product
MA1-70-2267 8 ml Stainless Steel Syringe with 1/16 inch tube fitting
MA1-70-2268 8 ml Stainless Steel Syringe with 1/8 inch tube fitting
HHaarrvvaarrdd HHiigghh PPrreessssuurree 88 mmll SSttaaiinnlleessss SStteeeell SSyyrriinnggee
This syringe has been designed to utilize the high forces available in our syringe
pumps to produce pressures up to 1500 psi. The syringe is constructed entirely of
#316 stainless steel with two Chemraz®O-Ring seals, two Teflon back-up rings and a
choice of 1/16 or 1/8 syringe to tube fitting. When used in the MA1-70-2200 or MA170-2201 Harvard PHD 4400, pressures up to of 1500 psi can be achieved for chromatography and process control applications.
Appendix B: Stainless Steel Syringes
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Appendix C: Min/Max Flow Rates
Minimum flow rates are taken from the smallest inside diameters and maximum flow
rates are taken from the largest inside diameters of the syringes supplied by the eleven
most widely used syringe manufacturers.
Appendix D: Custom Applications
The Harvard PHD 4400 Hpsi Syringe Pumps lend themselves to a multitude of OEM
indus
ttrrii
al applications. For all types of custom pumping or pilot plant applications, the
pump’s programming capa
bbiill
ities can be enhanced. Please contact the Harvard
Development Group if we can be of help.
Nominal Minimum/Maximum Flow Rates for Various Syringes
(Actual Limits will vary depending on manufacturer)
Syringe µl/hr µl/min ml/hr ml/min
Size Min. Max. Min. Max. Min. Max. Min. Max.
0.5 µl 0.0001 95.330
1 µl 0.0002 190.70
2 µl 0.0004 381.30
5 µl 0.0010 953.17
10 µl 0.0019 1.9013
25 µl 0.0046 4.7752
50 µl 0.0092 9.5511
100 µl 0.0183 19.153
250 µl 0.0454 47.532
500 µl 0.0911 95.492
1000 µl 0.0031 190.95
1 ml 0.0033 205.30
2 ml 0.0119 747.35
2.5 ml 0.0076 476.21
3 ml 0.010 11.231
5 ml 0.0208 21.781
10 ml 0.0301 31.486
20 ml 0.0523 54.804
30 ml 0.0673 70.518
50 ml 0.1019 106.76
100 0.1740 182.40
140 ml 0.2106 220.82
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Appendix E: Pressure & Force Specs
RRaattee
This is the rate indicated with a Harvard
Apparatus 50 ml stainless steel syringe.
Diameter 28.6 mm, cross-sectional area
1.00 square inches.
SSttaarrttiinngg PPSSII
This is the back pressure at which the
pump would reliably start. At higher
pressures, the pump may stall.
Rate Starting Stall Stall Force Stall Force
ml/min PSI PSI Lbs. (Kg)
10 >200 >200 >200 >90
20 >200 >200 >200 >90
30 >200 >200 >200 >90
50 >200 >200 >200 >90
60 >200 >200 >200 >90
70 >200 >200 >200 >90
80 >175 >200 >200 >90
90 >150 >175 >175 >79
100 >125 >150 >150 >68
110 >100 >125 >125 >56
120 >75 >100 >100 >45
Appendix F:
PHD 4400 Hpsi to PC Connection
3
RJ11/RJ12
6
RJ12 - 6 x 6
22/2000
5
4/
Front View
Figure 13. PHD 4400 Hpsi to PC Connection
SSttaallll PPSSII
This is the back pressure that finally stalls
the pump.
SSttaallll FFoorrccee
This is the force corresponding to the
stall PSI. The same force applied to different size syringes will generate correspondingly higher or lower pressures
and flow rates.
4400 Hpsi
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PPuummpp CChhaaiinn
Pump Port 1 – Computer control side
Pump Port 2 – Connection for remainder
of pump chain
Baud Rate – 1200, 2400, 9600 or 19,200
Word Size – 8
Parity – none
Stop Bits – 2
SSccaallee
Connect scale to Pump Port 1
Supported scales:
Mettler
Baud Rate – 2400
Word Size – 7
Parity – even
Stop Bits – 1
Pause – 0
Sartorius
Baud Rate – 2400
Word Size – 7
Parity – even
Stop Bits – 1
Code Settings:
311 – Weigh only (models with PLUS
performance package)
211 – External print command without stability
225 – 2400 baud
234 – Even Parity
432 – Beeper off
Appendix G: RS-232C Specifications
Figure 14. RS-232C Specifications
Ohaus
Baud Rate – 2400
Word Size – 8
Parity – even
Stop Bits – 1
Auto print feature – off
Stable data only – off
Serial data frame selection – Fr.7
PPrriinntteerr
Serial printer with print buffer:
Baud Rate – 2400
Word Size – 8
Parity – none
Stop Bits – 2
Symphony, the Windows
®
Pump Manager software, allows you to control your PHD
4400 Hpsi pump from your remote computer. With Symphony, you can orchestrate all
pump functions from a virtual control panel via the serial communications link built
into every PHD 4400 Hpsi pump.
Catalog Number Product
MA1-70-2200 PHD 4400 Hpsi Programmable Syringe Pump
MA1-70-2201 PHD 4400 Hpsi Remote Programmable Syringe Pump
MA1-70-3000 Symphony Software
Appendix H: Symphony
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Appendix I: User I/O Connector Specs
PPiinn AAssssiiggnnmmeennttss
1. Vss (Ground reference, logic low)
Falling edge starts/stops pump
2. Valve control output:
Low – Infusing
High – Refilling
3. Running Output Indicator
Low - Pumped stopped
High - Pump running
4. Programmable output
5. Logic High Reference (5V, 6 mA)
6. Foot switch input
7. Timer Input
Rising edge starts pump
Falling edge stops pump
8. Directional control input
Rising edge sets pump to infuse
Falling edge sets pump to refill
User I/O (input/output) connections
are via a 9 pin D-sub type connector. All
inputs are internally pulled up to a
logic high.
AAuuttoo FFiillll AAcccceessssoorryy
To operate the Auto Fill feature, either the low pressure or high pressure valve assembly is required. Each assembly contains the solenoid valve, connector cable, power
cable, and tubing and fittings to connect to the syringe. Operates at 115/230 VAC,
50/60 Hz via a selector switch.
Appendix J: Accessories & Spare Parts
Cat. No. Accessories
72-2748 Daisy-Chain Cable, 1.8 m (6 ft)
55-7760 Daisy-Chain Cable, 0.6 m (2 ft)
70-2022 Daisy-Chain Connector, 9 PIN
55-4145 Daisy-Chain Connector, 25 PIN
55-4144 Foot Switch
55-7002 ‘Auto Fill’ Valve Box–Regular
Pressure – to 20 psi
55-1145 ‘Auto Fill’ Valve Box–High Pressure
– 200 psi
5108-032 Adjustable Stop
Cat. No. Spare Parts
72-0199 Remote Extension Cable, 1.5 m (5 ft)*
72-1405 Remote Ext. Cable, 9.1 m (30 ft)*
0606-086 Magnalube - Lead Screw Lubricant
4430-034 Syringe Clamp
5100-060 Thumb Screw, Syringe Clamp
5100-059 T Knob, Withdraw & Anti-Siphon
Bracket
5153-209 Power Cord
5406-001 PHD 4400 Hpsi Operators Manual
5153-554 Fuse, 0.75 A, 250 V
* For PHD 4400 Hpsi Remote Syringe Pump
Figure 15.User I/O Connector Specifications
9. Event input
Falling edge triggers program event
Logic Low: 0 V – 0.8 V
Max. 6 mA current sink
Logic High: 2 V – 5 V
Max. 6mA current source
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MMaaiinntteennaannccee
PHD 4400 Hpsi series pumps require no special maintenance other than keeping them
clean by avoiding accidental spills of pumped material.
The two guide rods and the lead screw should be sparingly lubricated periodically. The
guide rods and the lead screw should be lubricated with Magnalube-G R grease
(Catalog # MA1-0606-086) provided with the pump.
Solvents of any type should never be used to clean the pump. A mild detergent solution may be used to clean the keypad.
TTrroouubblleesshhoooottiinngg
Infusion Accuracy
To ensure infusion accuracy always use new syringe(s) and measure syringe bore
diameter and enter actual dimensions in millimeters (mm). Additionally make
sure that the guide rods and lead screw are properly lubricated.
RS-232C Difficulties
Verify that the baud rates and data framing parameters on all devices are the
same. With the pump chain, a “Communication timeout” error on a computer is
usually caused by the computer errantly handshaking on the RTS, CTS and DSR
lines. Verify pins 4, 5 and 6, on the 25 pin connector, are jumped on the computer side of the cable.
PPHHDD 44440000 HHppssii DDiissppllaayy MMeessssaaggeess
Pump Stalled
This indicates that the pusher block travel has been impeded. This may be
caused by the syringe plunger hitting bottom, a kink in the tubing, syringe
plunger binding or any situation requiring more force to the head of the syringe
than the pump is capable of delivering. To restart the pump, remove the cause
of stalling and press RUN. The pump will continue the interrupted procedure
where it stopped. Also note, a large increase in the pumping rate could also stall
the motor.
Out of Range
A value was entered or encountered in a pump program that was beyond the
pump’s limits. Also, verify that the Infuse Rate is not 0. Note that rates must be
less than 42949. Express larger rates using different units.
EEPROM Data Lost
The values stored in the nonvolatile memory have been lost. This should never
occur under normal operation.
Replace NOVRAM
The nonvolatile memory has failed. This should never occur under normal operation.
Appendix K: Maintenance & Troubleshooting
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Appendix L: ‘Auto Fill’ Valves
There are two 'Auto Fill' Valve models available:
Catalog No. Product
55-7002 Supplied with 1/8 inch I.D., 1/4 inch O.D. silastic tubing and connector to syringe
55-1145 Supplies with 1/4 inch O.D. 304 stainless steel tubing with Swagelok fittings
SSeettttiinngg UUpp tthhee VVaallvvee
Set up consist of three phases: Voltage selection (110 or 220 V), Pump to valve electrical connection and Valve tubing to syringe hook up.
1. Voltage Selection
Valves are shipped from the factory with an American line cord with three wire molded power connector and the pump set for 115 - 125 VAC.
For 220 - 240 VAC operation the molded power connector must be cut off and
replaced with an appropriate type. The line cord is color coded in International standard colors:
BBrroowwnn -- HHiigghh BBlluuee -- NNeeuuttrraall GGrreeeenn -- GGrroouunndd
Observe the polarities when installing the new connector. To adjust the valve for 220
- 240 volt operation an international voltage selector switch must be set. Remove the
top panel by removing eight screws, four on each end of the valve. Identify the voltage selector switch located directly below. Using a screw driver turn the switch from
110 to 220. The pump is now set up for 220 volt operation.
2. Pump to Valve Electrical Connection
The cable fitted with a male 9 pin D-sub connector must be connected to the female
D-sub connector on the rear of the PHD 4400 Hpsi Syringe Pump. Power can now be
switched on with the illuminated power On/Off switch on the front valve housing.
3. Pump to Valve Tubing Connection
Swagelok fitting should be finger tight plus 1 1/4 turn. Tubing is 304 stainless steel 1/4
inch O.D. wall thickness 0.035 inch.
Silicon tubing 1/4 inch OD, 1/8 inch ID
Figure 16. Pump to Valve Tubing Connection
Declaration of Conformity
Application of
Council Directive(s): 73/23/EEC, 89/336/EEC
Standard(s) to which
conformity is declared: EN61010, EN50082-1, EN50081-1
Manufacturer’s Name: Harvard Apparatus, Inc.
Manufacturer’s Address: 84 October Hill Road
Holliston, Massachusetts 01746
U.S.A.
Type of Equipment: Syringe Pump
Model No.: 4400 Series
I, the undersigned, hereby declare that the equipment specified above
conforms to the above Directive(s) and Standard(s).
Place: United States of America
Date: September 7, 2001
Beth Bauman
VP Engineering / Operations
(Signature)
(Full Name)
(Position)
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