HARVARD CMA 11, CMA 7 User Manual

TECHNICAL INFORMATION

Membrane

Material

Polyethersulfone (PES)

Molecular Cut-O

500kDa, 2MDa

Outer Diameter

0.28 mm

Length

1, 2, 3 and 4 mm

Probe Shaft

Material

Stainless-steel

Diameter

0.40 mm

Length

14 mm

Internal Volume

Inlet Volume

0.1 µL

Outlet Volume

1 µL

CMA 11 55 kDa & 2MDa Microdialysis Probe User’s Manual

0.40

0.28

shaft

steel shaft

outletintlet

membrane

Instructions for CMA 11 500 kDa & 2MDa Microdialysis Probes

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

Note: The 500 kDa and 2 MDa Cut Off membrane has to be use in a Push-Pull system to avoid loss of fluid out to the
surrounding tissue (ultra-filtration). Here the use of a CMA syringe pump and REGLO ICC Pump is recommended.

Load the peristaltic pump with the "FEP Tubing Connector Peristaltic Kit". Adjust the lengths of the FEP tubing if needed for a
lower dead volume; recalculate the outlet volume if needed (1.2 µl/100 mm length)

Run the pump to make sure that liquid leaves the tip of the syringe cannula.

Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at
room temperature and preferably degassed.

Place the inlet end of the “FEP Tubing Connector Peristaltic Kit” in a beaker with Perfusion Fluid and flush the pump to fill all
tubing with perfusion fluid. Make sure there is no air bubbles in the complete tubing set.

Set the pump to the required perfusion flow, usually 1 – 5 µL/min.

Prepare a desired length of tubing with a Tubing Adapter on both ends and connect the tubing to the inlet of the probe.
Remove the protection tube from the probe carefully. Short cannula = inlet, long cannula = outlet. Tubing Adapters and FEP
tubing should be used for all connections. To facilitate the handling of Tubing Adapters, they should be soaked in Ethanol

70% for minimum 10 minutes.

Mount the probe in a Probe/Guide Clip on the CMA 130 In Vitro Stand. Put the probe membrane into a vial filled with
perfusion fluid. Connect the inlet tubing of the probe to the syringe cannula by sliding the Tubing Adapter over the cannula.

Flush the probe with perfusion fluid at 8-10µL/min for 4-5 min to wash out air. Knock on the shaft of the clip to help the air to
flush away. At this process the Ultra High Cut Off membrane will look as leaking but this is due to ultra-filtration of fluid
through the membrane.

Set the pump to the required perfusion flow, usually 1 – 5 µL/min. Make sure both pumps have the same flow rate.

Connect the inlet “FEP Tubing Connector Peristaltic Kit” at the peristaltic pump to the outlet of the probe

11.

12.

13.

Lift up the probe from the vial and check that the membrane doesn’t ultrafiltrate or dry out; it should look filled out but
not sweating. Lower the probe into the vial and control the outlet volume for the complete system.

The system with the probe is now ready for use.

When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION).
This causes a delay that must be calculated when using low perfusion rates and short sampling times

WARRANTY

The probes manufactured by CMA Microdialysis are warranted to be free from defects in material and workmanship for
a period of two years from the manufacturing date if stored in the original package.
Claims should be forwarded without delay to CMA Microdialysis or to your local distributor.

ORDER INFORMATION

Ref No.

CMA 11 500 kDa Microdialysis Probe, 1 mm, 3/pkg* CMA 8012521

CMA 11 500 kDa Microdialysis Probe, 2 mm, 3/pkg* CMA 8012522

CMA 11 500 kDa Microdialysis Probe, 3 mm, 3/pkg* CMA 8012523

CMA 11 500 kDa Microdialysis Probe, 4 mm, 3/pkg*

CMA 8012524

CMA 11 Guide Cannula, 3/pkg CMA 8309017

CMA 11 Guide Cannula, 30/pkg CMA 8309018

The CMA 11 Microdialysis Probe is not intended for use in humans. It is only suitable for laboratory research in animals.
CMA Microdialysis only guarantees single usage of CMA 11 Microdialysis Probes

CMA Microdialysis AB

Head Office, Sweden

Torshamnsgatan 30A, SE-164 40 Kista, Sweden
Tel: [phone]
E-mail: [e-mail]

Harvard Apparatus

US Office

84 October Hill Road
Holliston, MA 01746 USA
Phone Orders: 800-232-2380 • Fax: [phone]
E-mail: [e-mail]

www.microdialysis.com

8011683A

*β –Irradiated Probes are available as Custom Probes

Tubing Adapter, 10/pkg

CMA 3409500

FEP Tubing, 1 m, 1/pkg CMA 3409501

FEP Tubing, 1 m, 10/pkg CMA 8409501

FEP Tubing Connector Peristaltic Kit, 3/pkg CMA 8012518

CMA 11 & 12 Probe Clip CMA 8309013

CMA 11 2MDa Microdialysis Probe, 1 mm, 3/pkg* CMA 8012525

CMA 11 2MDa Microdialysis Probe, 2 mm, 3/pkg* CMA 8012526

CMA 11 2MDa Microdialysis Probe, 3 mm, 3/pkg* CMA 8012527

CMA 11 2MDa Microdialysis Probe, 4 mm, 3/pkg*

CMA 8012528

Microsyringe 1 mL

Microsyringe 2.5 mL

CMA 8309020

CMA 8309021

OPTIONAL ACCESSORIES

Ref No.

CMA 4004 Syringe Pump

CMA 402 Microdialysis Pump with Accessory Kit

CMA 400400

CMA 8003100

CMA 402 Microdialysis Pump

CMA 110 Liquid Switch

CMA 8003110

CMA 8308200

CMA 130 In-Vitro Stand with CMA 11&12 Clips

CMA 142 Microfraction Collector 230V

CMA 8309104

CMA 8381142

CMA 142 Microfraction Collector 115V

CMA 470 Refrigerated Fraction Collector

CMA 8381143

CMA 8002770

For other probes and microdialysis accessories please call
your local CMA Microdialysis dealer.