HARVARD 33, MA1-55-3333 User Manual
Syringe Pump Model 33 MA1-55-3333
Model 33
Twin Syringe Pump
User's Manual
Publication 5390-001 Revision B
Publication 5390-001 Revision B
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
SUBJECT PAGE
Table of Contents
Table of Contents ..............................................................1
Warranty and Repair Information ....................................2
Specifications ....................................................................3
Features ..............................................................................4
Initial Setup ........................................................................5
Loading Syringes ..............................................................6
User Interface..................................................................7-8
Description of Keys ......................................................8
Entering Data......................................................................9
Operation ..........................................................................10
Diameters ....................................................................10
Pumping Rates............................................................10
Selecting the Run Mode..............................................10
Selecting the Pumping Directions ..............................10
Running the Pump ......................................................10
Single Syringe Operation ............................................10
Valve Control Box ............................................................11
Pinch Valve Connectors ............................................12-13
External Control and Interfaces ................................14-15
Attaching/Configuring a Pump Chain ..........................14
TTL Devices ................................................................15
Foot Switch Input ........................................................15
Pump Chain Commands ............................................16-20
Pump Commands and Responses ............................17
Pump Chain Error Messages ......................................20
Appendices..................................................................21-27
A Syringe Diameters in mm....................................21
B Nominal Min/Max Flow Rates ............................22
C Command Information ........................................23
D RS-232 Specifications ........................................24
E TTL Connector Specifications ............................24
F Pump to PC Connection ....................................25
G Maintenance........................................................26
H Troubleshooting ..................................................27
I Accessories ........................................................28
J Custom Applications............................................28
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
CAUTION: Not for clinical use on human patients.
Warranty and Repair Information
Serial Numbers
All inquires concerning our product should refer to the serial number of the unit.Serial
numbers are located on the rear of the chassis.
Calibrations
All electrical apparatus is calibrated at rated voltage and frequency.While the flow will
stay calibrated, the peak will vary.
Warranty
Harvard Apparatus warranties this instrument for a period of one year from date of purchase.At its option, Harvard Apparatus will repair or replace the unit if it is found to be
defective as to workmanship or material.
This warranty does not extend to damage resulting from misuse,neglect or abuse, normal
wear and tear, or accident.This warranty extends only to the original customer purchaser.
IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR
CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitation of inci-
dental or consequential damages so the above limitation or exclusion may not apply to
you.THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS
FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow
this limitation on an implied warranty, so the above limitation may not apply to you.
If a defect arises within the two-year warranty period, promptly contact Harvard
Apparatus, Inc. 84 October Hill Road, Building 7, Holliston, Massachusetts
01746-1371 using our toll free number 1-800-272-2775, or outside the U.S. 508-893-
8999. Goods will not be accepted fir return unless an RMA (returned materials authorization) number has been issued by our customer service department.The customer is
responsible for shipping charges. Please allow a reasonable period of time for completion of repairs, replacement and return. If the unit is replaced, the replacement unit is
covered only for the remainder of the original warranty period dating from the purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary
from state to state.
Repair Facilities and Parts
Harvard Apparatus stocks replacement and repair parts.When ordering, please describe
parts as completely as possible, preferably using our part numbers. If practical, enclose
a sample or drawing.We offer a complete reconditioning service.
CAUTION
This pump is not registered with the FDA and is not for clinical use on human patients.
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
Specifications
Harvard Pump 33 Specifications
Accuracy ±0.35%
Reproducibility ±0.1%
Type Dual Syringe Infusion/Withdrawal Parallel/Reciprocal
Size, L x W x H (286 x 311 x 152 mm) 11.25 x 12.25 x 6 in
Weight (6.8 kg) 15 lbs
Power 115/230 VAC, 50-60 Hz via selector switch, 45 W
Fuse 1 A (115 V), 0.5 A (220 V)
Leakage to Ground Typically < 10 µA
Ground Resistance Typically < 0.06 Ω
Voltage Operating Range 95-130 VAC, 220-260 VAC
Drive Motors 2 motors, each 0.9 degree steppers
Motor Drive Circuitry Microprocessor controlled from 1/2 to 1/4 microstepping
Timing Belt Drive 2:1
Lead Screw Pitch 24 threads/inch
Motor Steps per One 1600 at 1/2 stepping or 3200 at 1/4 stepping
Revolution of Lead Screw
Step Rate:
Minimum 27.3 sec/step
Maximum 416.7 µsec/step
Pusher Advance per Step:
Minimum 0.330729 µm
Pusher Travel Rate:
Minimum 0.726699 µm /min
Maximum 95.25 mm/min
Dynamic Speed Range 131,072 to 1
Force 40 lbs max.
Pressure 99.5 PSI max. with 20 ml stainless steel syringe
Calibration Enter syringe diameter, any size, any make, up to 50 mm
diameter
Syringe Size 10 µl (min.), 50 ml (max.)
Flow Rate Range:
Minimum 0.0073 µl/hr (10 µl syringe)
Maximum 53.346 ml/min (50 ml syringe)
Display 5 digits plus 14 indicator LEDs
RS-232 Interface Chained dual bidirectional ports
Non-Volatile Memory Storage of all settings
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
Features
Pressure and Speed
Pump 33 can deliver up to 53.346 ml/min with a 50 ml syringe,and is capable of pressures of up to 99.5 PSI with a 20 ml syringe.
Independent Syringe Diameters and Rates
In the Proportional Mode, separate syringe types and rates may be set.
Variable Syringes
Enter the inside diameter of the syringes being used.
Modes of Operation
Auto Stop
Both syringes will operate at same rate and stop when either syringe reaches a
limit stop.
Continuous Run
With both syringes operating at same rate,when end of travel is reached, the pump
automatically reverses the direction of travel for both syringes.The external valves,
optionally attached to the syringes, will be automatically activated or deactivated.
Proportional
Pump operates as in Auto Stop,but independent rates and diameters are set for each syringe.
Parallel/Reciprocal Operation
Syringes may pump in the same direction (parallel) or opposite directions (reciprocal).
External Connections
TTL
Allows pump operations to be synchronized with external devices or by a person
at a distance from the pump. Direction of pump travel can be set via a TTL pin.
Also,TTL pins are used to control an external valve for refilling and provide an output to indicate whether or not the pump is running. Additional TTL pins are available for general use.
RS-232
Multiple pumps can be chained together and remotely controlled from a computer or
any device communicating via RS-232.
Valves
Models MA1-55-3334 and MA1-55-3335 include a valve assembly which allows automatic selection of infusion and refill lines.
Non-Volatile Memory
All operational data entered into the pump from the keypad or requested to be saved
via RS-232, will be stored. On power up, all ettings from when it was powered down
will be recalled and the display will blink until the pump receives its first command.
Stall Detection
An optical detector on each motor is used to verify expected movement of the motors.
If motor is prevented from turning due to jamming or kinking of the tubing,the pump
stops and the display indicates a stall condition by blinking the run light and the
syringe direction light of the stalled syringe. Pump 33 employs a microcontroller
which controls a small step angle stepping motor that drives a lead screw and half nut.
Microstepping techniques are used to further reduce the step angle making flow pulsation negligible. A keypad is used for entry of operating data to the pump or data can
be entered via RS-232. The microcontroller calculates the cross-sectional area of the
syringe selected and calibrates the flow rate.
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
Initial Setup
1. Read the manual.
2. Locate the voltage selector switch on the rear panel of the pump and set it to the
voltage being used. If other than 115VAC, 60 Hz is being used, the plug must be
cut off and an appropriate plug installed, observing the polarity of the international line cord used:
Brown – high
Blue – neutral
Green – ground
3. Turn on main power switch located directly above the line cord on the rear
panel.The display will illuminate indicating that the power connections are correct.The flashing display indicates that power has just been applied.
4. Load syringes.
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
SYRINGE 1
SYRINGE 2
REFILL
LIMIT STOP
INFUSE
LIMIT STOP
LIMIT
SWITCHES
1
3
4
2
1. The syringe holder and pusher block are fitted with movable retaining brackets
which hold the syringe barrel and plunger when refilling.When loading the
syringe into the pump, it is necessary to adjust these brackets.The pusher block
is fitted with a mechanism to release the drive nut from the leadscrew.This
allows the block to move freely so the syringe to be loaded.
2. Loosen the screws on the syringe block and pusher block to free the retaining
brackets (2 and 3, Figure 1)
3. To free the pusher block from the leadscrew, turn the knob on the front of the
block (1) until the pin in the knob slips into the hole in the block.
4. The syringe clamp locking screw on the right side of the syringe block (4)
should be loosened and the clamp rotated to the side.
5. Place the syringe barrel on the syringe holder block and move the pusher block
to accommodate the plunger.
6. Make sure the syringe barrel flange and the plunger flange are held by the retain-
ing clamps. Press the retaining brackets firmly against the flanges and tighten the
retaining screws.
7. Rotate the syringe clamp and press down firmly on the syringe barrel. Secure in
place by tightening the locking screw (4).
Setting Limit Stops
To set the limit stops, loosen thumbscrews on limit stops and slide stops to desired
travel limits (infuse limit & refill), tighten thumbscrews.
Loading Syringes
Figure 1. Syringe Loading
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
User Interface
Auto Stop
Proportional
Continuous
Remote
Run
ml/min
µl/min
ml/hr
µl/hr
mm
Syringe 1
Syringe 2
The user interface consists of a display area and a keypad. The display consists of a 5
digit LED display and 14 LED indicators. The display will be showing either the default
display or an informational message. The two syringes are referred to as Syringe 1 and
Syringe 2. Syringe 1 is located towards the front and Syringe 2 is located towards the
rear. Rate 1 refers to the set flow rate of Syringe 1 and Rate 2 refers to Syringe 2.
By default, the display will be showing Rate 1. Informational messages are shown
when the user queries another data value by pressing the corresponding key, or when
the pump is alerting the user to a problem. Such problems are a value out of range
(oor) or the pump stalling (StALL).
The 14 LED indicators are divided into three sections: Pump mode and state, syringe
directions, and units of value being displayed. See Figure 2.The LED indicators are
as follows:
Pumping Mode and State LEDs
Meaning
Auto Stop Pump is in Auto Stop mode
Proportional Pump is in Proportional mode
Continuous Pump is in Continuous mode
Remote Pump Chain communication
Run At least one motor is operating
Syringe Directions LEDs
Comprising of 4 indicators, these LED’s indicate the direction of the syringes when
illuminated. When blinking, indicates the corresponding motor is stalled, or data pertaining to that syringe is being entered.
Unit LEDs
Meaning
ml/mn milliliters per minute
µl/mn microliters per minute
ml/hr milliliters per hour
µl/hr microliters per hour
mm millimeters
Figure 2. User Interface
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Harvard Apparatus Model 33 Twin Syringe Pump User's Manual
User Interface: Description of Keys
Set
Rate
1
1
7
2
8
3
9
4
0
5
.
6
Enter
HARVARD
‘33’ Syringe Pump
Data Entry
Diameter
1
Rate
2
Diameter
2
Stop
Run
Change
Direction
Parallel
Reciprocal
Select
Mode
Proportional Only
Set Keys
Toggle
The keypad consists of 21 keys used for entering control information and data into the
pump. These keys are grouped into 3 sections (see Figure 3 above): Set keys, toggle
keys, and data entry keys.
Set Keys
Set Allows modification of a data item in this group of keys.To
modify a data item, press the relevant key after pressing the
SET key.
Rate 1 Displays/sets current rate of syringe 1. Also syringe 2 rate if
current mode is not Proportional. Scrolls through rate units
when setting.
Rate 2 Displays/sets current rate of syringe 2. Scrolls through rate
units when setting. Key only valid in Proportional mode.
Diameter 1 Displays/sets current diameter of syringe 1. Also diameter of
syringe 2 if current mode is not Proportional.
Diameter 2 Displays/sets current diameter of syringe 2. Key valid in
Proportional mode.
“1” Used to display and set current Pump Chain address and to
scroll through supported baud rates while setting.
Toggle Keys
Parallel/ Chooses between Parallel and Reciprocal pumping operation.
Reciprocal
Select Mode Chooses between Proportional, Continuous Run, or Auto Stop.
LED indicators display current mode. Pump must be stopped
to change modes.
Change Reverses direction of both syringes and sets the valve control
Direction output as appropriate.
Run/Stop Starts or stops the pump.
Data Entry Keys
1 through 0, . Used to facilitate the entering of numeric data values.
Enter Stores displayed data value when entering data.
Figure 3. Keypad Interface
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