HANNA LITERATUREHANNA LITERATURE
HANNA LITERATURE
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FISH FARMING WATER ANALYSIS
Instruction Manual
HI 93732N
MICROPROCESSOR-BASED
DISSOLVED OXYGEN
SPECTROPHOTOMETER
GENERAL CATALOG
ENVIROCARE
These and many others catalogs, handbooks and leaflets are available from Hanna. To receive your free copy, contact your dealer or the
nearest Hanna Customer Service Center.
HTTP://WWW.HANNAINST.COM
PRINTED IN ITALY
MAN93732R1
07/97
http://www.hannainst.com
This Instrument is in Compliance
with the CE Directives
Dear Customer,
Thank you for choosing a Hanna product.
Please read this instruction manual carefully before using the meter.
This manual will provide you with the necessary information for a
correct use of the instrument.
If you need additional technical information, do not hesitate to e-mail
us at tech@hannainst.com.
This instrument is in compliance with the directives EN 50081-1
and EN 50082-1.
TABLE OF CONTENTSTABLE OF CONTENTS
TABLE OF CONTENTS
TABLE OF CONTENTSTABLE OF CONTENTS
PRELIMINARY EXAMINATION ........................................................ 3
GENERAL DESCRIPTION ................................................................ 3
PRINCIPLE OF OPERATION ...........................................................4
DISPLAY CODE GUIDE .................................................................. 6
TIPS FOR AN ACCURATE MEASUREMENT ....................................... 7
FUNCTIONAL DESCRIPTION & SPECIFICATIONS.............................. 8
OPERATIONAL GUIDE ................................................................... 9
BATTERY REPLACEMENT .............................................................. 11
OTHER SPECTROPHOTOMETERS FROM HANNA ............................ 12
ACCESSORIES ............................................................................. 13
WARRANTY ................................................................................ 13
CE DECLARATION OF CONFORMITY.............................................. 15
CE DECLARATION OF CONFORMITYCE DECLARATION OF CONFORMITY
CE DECLARATION OF CONFORMITY
CE DECLARATION OF CONFORMITYCE DECLARATION OF CONFORMITY
ISO 9000 Certified
Company since 1992
2
Recommendations for Users
Before using this product, make sure that it is entirely suitable for the environment in which it is
used.
Operation of this instrument in residential areas could cause unacceptable interferences to radio and TV
equipments, requiring the operator to take all necessary steps to correct interferences.
Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC
performance.
To avoid damages or burns, do not perform any measurement in microwave ovens.
15
OTHER PRODUCTS FROM HANNAOTHER PRODUCTS FROM HANNA
OTHER PRODUCTS FROM HANNA
OTHER PRODUCTS FROM HANNAOTHER PRODUCTS FROM HANNA
PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION
PRELIMINARY EXAMINATION
PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION
• CALIBRATION AND MAINTENANCE SOLUTIONS
• CHEMICAL TEST KITS
• CHLORINE METERS
• CONDUCTIVITY/TDS METERS
• DISSOLVED OXYGEN METERS
• HYGROMETERS
• MAGNETIC STIRRERS
• Na/NaCl METERS
• pH/ORP/Na ELECTRODES
• pH METERS
• PROBES (DO, µS/cm, RH, T, TDS)
• PUMPS
• REAGENTS
• SOFTWARE
• THERMOMETERS
• TITRATORS
• TRANSMITTERS
• TURBIDITY METERS
• Wide Range of Accessories
Most Hanna meters are available in the following formats:
• BENCH-TOP METERS
• POCKET-SIZED METERS
• PORTABLE METERS
• PRINTING/LOGGING METERS
• PROCESS METERS (Panel and Wall-mounted)
• WATERPROOF METERS
• METERS FOR FOOD INDUSTRY
Remove the instrument from the packing material and examine it
carefully to make sure that no damage has occurred during shipment. If there is any damage, notify your Dealer or the nearest
Hanna Customer Service Center.
HI 93732N is supplied complete with:
• Two Sample Cuvets and Caps
• One Transport Cap
• One 60 mL BOD Bottle
• 9V Battery
Note: Save all packing material until you are sure that the instru-
ment functions correctly. Any defective item must be returned
in its original packing with the supplied accessories.
GENERAL DESCRIPTIONGENERAL DESCRIPTION
GENERAL DESCRIPTION
GENERAL DESCRIPTIONGENERAL DESCRIPTION
HI 93732N is a portable, microprocessor-based spectrophotometer
that measure the content of dissolved oxygen in surface, feed, natural
and waste waters.
The meter uses an exclusive positive-locking system to ensure that the
cuvet is in the same place every time it is placed into the measurement cell.
The reagents are in liquid form and are supplied in bottles. The
amount of reagent is precisely dosed to ensure maximum repeatability. Display codes aid the user in routine operations. The meter has
an auto-shut off feature that will turn itself off after 10 minutes of
non-use.
For additional information, contact your dealer or the nearest Hanna
Customer Service Center.
You can also e-mail us at tech@hannainst.com.
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PRINCIPLE OF OPERATIONPRINCIPLE OF OPERATION
PRINCIPLE OF OPERATION
PRINCIPLE OF OPERATIONPRINCIPLE OF OPERATION
ACCESSORIESACCESSORIES
ACCESSORIES
ACCESSORIESACCESSORIES
The color of every object we see is determined by a process of
absorption and emission of the electromagnetic radiation (light) of its
molecules.
Colorimetric analysis is based on the principle that specific compounds
react with others and form a color, the intensity of which is
proportional to the concentration of the substance to be measured.
When a substance is exposed to a beam of light of intensity Io, a
portion of the radiation is absorbed by the substance's molecules and
a radiation of intensity I, lower than Io, is emitted.
The quantity of radiation absorbed is given by the Lambert-Beer
Law:
log Io/I = ελ c d
Where log Io/I = Absorbance (A)
ε
= molar extinction coefficient of the substance at
λ
wavelength λ
c = molar concentration of the substance
d = optical distance light travels through sample
Therefore, the concentration "c" can be calculated from the color
intensity of the substance determined by the emitted radiation I, as
the other factors are known.
LED
EMITTED LIGHT
CUVET MICROPROCESSOR
BLOCK DIAGRAM OF A SPECTROPHOTOMETER
LIGHT
DETECTOR
A monochromatic LED (Light Emitting Diode) emits radiation at a
single wavelength, supplying the system with the intensity Io.
Since a substance absorbs the color complimentary to the one it emits
(for example, a substance appears yellow because it absorbs blue
light), Hanna spectrophotometers use LEDs that emit the appropriate
wavelength to measure the sample.
The optical distance (d) is measured by the diameter of the cuvet
containing the sample.
The photoelectric cell collects the radiation I that is not absorbed by
the sample and converts it into an electric current, producing a
potential in the mV range.
HI 93732-01 Reagent kit for 100 D.O. tests
HI 93732-03 Reagent kit for 300 D.O. tests
HI 710009 Blue rubber boot
HI 710010 Orange rubber boot
HI 93700-C Measurement cuvets (4 pcs)
HI 93703-50 Cleaning solution for cuvets (230 mL)
HI 93703-70 Tissue for wiping cuvets (4 pcs)
MAN93732R1 Instruction manual
WARRANTYWARRANTY
WARRANTY
WARRANTYWARRANTY
All Hanna Instruments meters are warranted for two years
against defects in workmanship and materials when used for their
intended purpose and maintained according to instructions.
This warranty is limited to repair or replacement free of charge.
Damages due to accidents, misuse, tampering or lack of prescribed
maintenance are not covered.
If service is required, contact the dealer from whom you purchased the
instrument. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair
is not covered by the warranty, you will be notified of the charges
incurred.
If the instrument is to be returned to Hanna Instruments, first obtain
a Returned Goods Authorization Number from the Customer Service
department and then send it with shipment costs prepaid. When
shipping any instrument, make sure it is properly packaged for
complete protection.
To validate your warranty, fill out and return the enclosed warranty
card within 14 days from the date of purchase.
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
Hanna Instruments reserves the right to modify the design,
construction and appearance of its products without advance notice.
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OTHER SPECTROPHOTOMETERSOTHER SPECTROPHOTOMETERS
OTHER SPECTROPHOTOMETERS
OTHER SPECTROPHOTOMETERSOTHER SPECTROPHOTOMETERS
FROM HANNAFROM HANNA
FROM HANNA
FROM HANNAFROM HANNA
Single-parameter Spectrophotometers
Description Code Range Method
Aluminum HI 93712 0.00 to 1.00 mg/L Aluminon
Ammonia LR HI 93700 0.00 to 3.00 mg/L Nessler
Ammonia HR HI 93715 0.00 to 9.99 mg/L Nessler
Bromine HI 93716 0.00 to 8.00 mg/L DPD
Chlorine, Free HI 93701 0.00 to 2.50 mg/L DPD
Chlorine, Total HI 93711 0.00 to 3.50 mg/L DPD
Chlorine Dioxide HI 93738 0.00 to 2.00 mg/L Chlorophenol Red
Chromium VI, LR HI 93749 0 to 300 µg/L Diphenylcarbohydrazide
Chromium VI, HR HI 93723 0 to 1000 µg/L Diphenylcarbohydrazide
Color of Water HI 93727 0 to 500 PCU Colorimetric Platinum Cobalt
Copper, LR HI 93747 0 to 990 µg/L Bicinchoninate
Copper, HR HI 93702 0.00 to 5.00 mg/L Bicinchoninate
Cyanide HI 93714 0.000 to 0.200 mg/L Pyridine-Pyrazalone
Cyanuric Acid HI 93722 0 to 80 mg/L Turbidimetric
Fluoride HI 93729 0.00 to 2.00 mg/L SPADNS
Hardness, Ca HI 93720 0.00 to 2.70 mg/L Calmagite
Hardness, Mg HI 93719 0.00 to 2.00 mg/L Colorimetric
Hardness, Total HI 93725 0.00 to 4.70 mg/L Calmagite/colorimetric
Hydrazine HI 93704 0 to 400 µg/L p-Dimethylaminobenzaldehyde
Iodine HI 93718 0.0 to 12.5 mg/L DPD
Iron, LR HI 93746 0 to 400 µg/L TPTZ
Iron, HR HI 93721 0.00 to 5.00 mg/L Phenantroline
Manganese, LR HI 93748 0 to 300 µg/L PAN
Manganese, HR HI 93709 0.0 to 20.0 mg/L Periodate Oxidation
Molybdenum HI 93730 0.0 to 40.0 mg/L Mercaptoacetic Acid
Nickel LR HI 93740 0.000 to 1.000 mg/L Photometric
Nickel HR HI 93726 0.00 to 7.00 g/L Photometric
Nitrate HI 93728 0.0 to 30.0 mg/L Cadmium Reduction
Nitrite, LR HI 93707 0.00 to 0.35 mg/L Diazotation
Nitrite, HR HI 93708 0 to 150 mg/L Ferrous Sulfate
pH HI 93710 5.9 to 8.5 pH Phenol Red
Phosphate, LR HI 93713 0.00 to 2.50 mg/L Ascorbic Acid
Phosphate, HR HI 93717 0.0 to 30.0 mg/L Amino Acid
Phosphorus HI 93706 0.0 to 15.0 mg/L Amino Acid
Silica HI 93705 0.00 to 2.00 mg/L Heteropoly Blue
Silver HI 93737 0.000 to 0.600 mg/L Cadion 2B
Zinc HI 93731 0.00 to 3.00 mg/L Zincon
Multi-parameter Spectrophotometers
Code Parameters
HI 93710 Free & Total Cl, pH
HI 93724 CYS, pH
HI 93725 Total Hardness, pH
HI 93741 Fe LR, Total Hardness
HI 93742 Fe LR, Mn LR
HI 93743 Fe LR, pH
HI 93744 Fe LR, Total Hardness, pH
HI 93745 Free & Total Cl, Fe LR, Total Hardness, pH
C 101 Br, Free & Total Cl, CYS, Fe LR, I, pH
C 104 Free & Total Cl, CYS, pH
The microprocessor uses this potential to convert the incoming value
into the desired measurement unit and to display it on the LCD.
The measurement process is done in two phases: setting the meter to
zero and the actual measurement.
The cuvet has a very important role because it is an optical element,
and thus requires particular attention.
It is important that both the measurement and the calibration
(zeroing) cuvets are optically identical to provide the same measurement conditions. Whenever possible use the same cuvet for both.
It is also necessary that the cuvet's surface is clean and not scratched,
in order to avoid measurement interference due to unwanted reflection and absorption of light.
It is recommended not to touch the cuvet walls with hands.
Furthermore, in order to maintain the same conditions during the
zeroing and the measuring phases, it is necessary to close the cuvet
to prevent any contamination.
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5
DISPLAY CODE GUIDEDISPLAY CODE GUIDE
E
DISPLAY CODE GUIDE
DISPLAY CODE GUIDEDISPLAY CODE GUIDE
This indicates that the meter is in a ready state
and zeroing can be performed.
Sampling in Progress. This prompt appears each
time the meter is performing a measurement.
This indicates that the meter has been zeroed
and measurement can be performed.
A zero reading was not taken. Insert a sample
before adding reagent and press ZERO.
Under range. A blinking "0.00" indicates that the
sample has absorbed less light than the zero
reference. Check the procedure and make sure you
use the same cuvet for reference (zero) and measurement.
Over range. A flashing value higher than the
maximum concentration of the meter (see specifications) indicates that the sample has absorbed less
light than the zero reference. Check the procedure
and make sure you use the same cuvet for reference (zero) and measurement.
Light over range. The cuvet is not inserted correctly
and an excess ambient light is reaching the
detector. If the cover is properly installed, then
contact your dealer or the nearest Hanna Customer
Service Center.
Light under range. The zero sample is too dark for
proper zeroing. If this is not the case, contact your
dealer or the nearest Hanna Customer Service
Center.
The "V" indicates that the battery voltage is
getting low and the battery needs to be replaced.
This indicates that the battery is dead and must
be replaced.
Note: once this indication is displayed, the meter
will lockup. Change the battery to restart.
• Press READ and "SIP" will appear during measurement.
• The instrument will then directly display the concentration of dissolved oxygen in mg/L.
INTERFERENCES
Interferences may be caused by reducing and oxidizing materials.
NaN
(Sodium azide) is added to prevent interferences from NO
3
BATTERY REPLACEMENTBATTERY REPLACEMENT
BATTERY REPLACEMENT
BATTERY REPLACEMENTBATTERY REPLACEMENT
To prolong the battery life, switch your meter off after use. The meter
also has an auto-shut off feature that will turn itself off after 10
minutes of non-use.
A "V" on the LCD indicates low voltage and, when
V
it appears, the battery should be replaced.
If the battery is not replaced, "-BA-" is displayed
soon afterwards. This is to prevent erroneous readings due to low voltage. The meter will then
lockup and will not accept any commands. The
display will also go blank.
Battery replacement must only take place in a non-hazardous area
using a 9 V alkaline battery.
Simply slide off the battery cover on the
back of the meter. Detach the battery from
the terminals and attach a fresh 9 V
9V
battery while paying attention to the correct polarity. Replace the battery and the
cover.
The meter will turn on automatically when a new
battery is connected. You can turn it off by pressing
ON/OFF.
ON
OFF
–
2
SLID
OFF
.
6
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• Let the sample stand and the flocculant agent will
start to settle.
• After approximately 2 minutes and when the
upper half of the bottle becomes limpid, add 10
drops of HI 93732C.
• Replace the cap and swirl the solution. The sample is ready for
measurement when it is yellow and completely limpid.
1.5 cm
• Fill the cuvet up to 1.5 cm (¾”) below the rim with
10 mL of the unreacted (original) sample, and
replace the cap. This is the blank.
• Place the cuvet into the holder and
ensure that the notch on the cap is
positioned securely into the groove.
• Press ZERO and “SIP” will appear on the display.
ZERO
• Wait for a few seconds and the display will
show “-0.0-”. Now the meter is zeroed and
ready for measurement.
• Remove the cuvet and dispose of the blank.
• Rinse the cuvet with some of the reacted
sample. Then, fill it up to 1.5 cm (¾”)
below the rim with 10 mL of the reacted
sample and replace the cap.
TIPS FOR AN ACCURATE MEASUREMENTTIPS FOR AN ACCURATE MEASUREMENT
TIPS FOR AN ACCURATE MEASUREMENT
TIPS FOR AN ACCURATE MEASUREMENTTIPS FOR AN ACCURATE MEASUREMENT
The instruction listed below should be carefully followed during testing
to ensure best accuracy.
• Do not let the test sample stand too long after reagent is added
or accuracy will be lost.
• Whenever the cuvet is placed into
the measurement cell, it must be
completely free of fingerprints, oil
or dirt. Wipe it thoroughly with
HI 93703-70 or a lint-free cloth
prior to insertion.
• It is important that the sample
does not contain any debris. This
would corrupt the readings.
• Each time the cuvet is used, the cap must be tightened to the
same degree.
• It is possible to take multiple readings in a row, but it is
recommended that a zero reading be taken for each sample and
that the same cuvet is used for zeroing and measurement.
• It is important to discard the sample immediately after the
reading is taken because the glass might become permanently
stained.
• Shaking the cuvet can generate bubbles in the sample, causing
higher readings. To obtain accurate measurements, remove such
bubbles by swirling or by gently tapping the vial.
• All the reaction times reported in this manual are referred to
20°C (68°F). As a general rule of thumb, they should be doubled
at 10°C (50°F) and halved at 30°C (86°F).
• Reinsert the cuvet into the instrument.
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7
FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION
E
FUNCTIONAL DESCRIPTION
FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION
& SPECIFICATIONS& SPECIFICATIONS
& SPECIFICATIONS
& SPECIFICATIONS& SPECIFICATIONS
OPERATIONAL GUIDEOPERATIONAL GUIDE
OPERATIONAL GUIDE
OPERATIONAL GUIDEOPERATIONAL GUIDE
INITIAL PREPARATION
HI 93732N is supplied complete with a 9 V
battery. Slide off the battery compartment
cover on the back of the meter and install
the battery while paying attention to its
polarity.
REQUIRED REAGENTS
Code Description Quantity
HI 93732A-0 Reagent A 5 drops
HI 93732B-0 Reagent B 5 drops
HI 93732C-0 Reagent C 10 drops
MEASUREMENT PROCEDURE
• Turn the meter on by pressing ON/OFF.
• When the LCD displays "- - -", it is ready.
9V
SLID
OFF
ON
OFF
Range 0.0 to 10.0 mg/L (ppm)
Resolution 0.1 mg/L
Accuracy ±0.2 mg/L ±3% of reading
Typical EMC ± 0.1 mg/L
Deviation
Light Source Light Emitting Diode @ 430 nm
Method Adaptation of the Standard Methods for the
Examination of Water and Wastewater (18
edition), azide modified Winkler method. The
reaction between dissolved oxygen and the reagent causes a yellow tint in the sample.
Light Life Life of the instrument
Light Detector Silicon Photocell
th
• Fill one 60 mL BOD bottle completely with the unreacted
sample.
• Replace the cap and ensure that a small part of the sample spills
over. This is to make sure that no air bubbles have been trapped
inside.
• Remove the cap and add 5 drops
of HI 93732A and 5 drops of
HI 93732B. Replace the cap again
and ensure that a part of the
sample spills over.
• Swirl the solution. The sample becomes orange and a flocculant
agent will appear.
Environment 0 to 50°C (32 to 122°F);
max 95% RH non-condensing
Battery Type/Life 1 x 9 volt/40 hours
Auto-Shut off After 10' of non-use
Dimensions 180 x 83 x 46 mm (7.1 x 3.3 x 1.8")
Weight 290 g (10 oz.)
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