Hanna Instruments HI 4522 Instruction Manual

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99 Washington Street Melrose, MA 02176

Phone 781-665-1400 Toll Free 1-800-517-8431

Instruction Manual

Visit us at www.TestEquipmentDepot.com

HI 4521 & HI 4522

pH/mV/ISE/Temperature/

Conductivity/Resistivity/TDS/Salinity

Bench Meters

OTHER ACCESSORIES

HI 710005/8 Voltage adapter from 115 VAC / 12 VDC 800 mA (USA plug) HI 710006/8 Voltage adapter from 230 VAC / 12 VDC 800 mA (European plug) ChecktempC Pocket-size thermometer (range –50.0 to 150.0 ºC) HI 76404N Electrode holder HI 8427 pH and ORP electrode simulator with 1 m (3.3') coaxial cable ending in female BNC

connectors

HI 931001 pH and ORP electrode simulator with LCD and 1 m (3.3') coaxial cable ending in female BNC

connectors

HI 76312 Platinum 4-ring conductivity/TDS probe with temperature sensor and 1 m (3.3') cable HI 7662-T Temperature probe with 1 m (3.3') cable HI 92000 Windows® compatible software HI 920010 9 to 9-pin RS232 cable

RECOMMENDATIONS FOR USERS

Before using these products, make sure they are entirely suitable for the environment in which they are used. Operation of these instruments in residential areas could cause unacceptable interferences to radio and TV

equipment, requiring the operator to follow all necessary steps to correct interferences. The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges. Avoid touching this glass

bulb at all times. During operation, ESD wrist straps should be worn to avoid possible damage to the electrode by electrostatic

discharges. Any variation introduced by the user to the supplied equipment may degrade the instruments’ EMC

performance. To avoid electrical shock, do not use these instruments when voltages at the measurement surface exceed

24 VAC or 60 VDC. To avoid damage or burns, do not perform any measurement in microwave ovens.

2 115

ORP ELECTRODES

HI 3131B

Glass-body, refillable, combination platinum ORP electrode. Use: titration.

HI 3230B

Plastic-body, gel-filled, combination platinum ORP electrode. Use: general purpose.

Dear Customer, Thank you for choosing a Hanna Instruments product. This manual will provide you with the necessary information for correct use of the instrument. Please read this instruction manual carefully before using the instrument.

These instruments are in compliance with directives.

WARRANTYWARRANTY

WARRANTY

WARRANTYWARRANTY

HI 4430B

Plastic-body, gel-filled, combination gold ORP electrode. Use: general purpose.

Consult the Hanna General Catalog for more electrodes with screw-type or BNC connectors.

EXTENSION CABLE FOR SCREW-TYPE ELECTRODES (SCREW TO BNC ADAPTER)

HI 7855/1 Extension cable 1 m (3.3') long HI 7855/3 Extension cable 3 m (9.9') long

114

HI 4521 and HI 4522 are warranted for two years against defects in workmanship and materials when used for their intended purpose and maintained according to instructions. Electrodes and probes are guaranteed for six months. This warranty is limited to repair or replacement free of charge.

Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact the dealer from whom you purchased the instrument. If under warranty, report

the model number, date of purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization number from the Technical Service Department and then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for complete protection.

To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase.

TABLE OF CONTENTSTABLE OF CONTENTS

TABLE OF CONTENTS

TABLE OF CONTENTSTABLE OF CONTENTS

WARRANTY ...................................................................................................................................... 3

PRELIMINARY EXAMINATION .............................................................................................................. 5

GENERAL DESCRIPTION ...................................................................................................................... 6

FUNCTIONAL DESCRIPTION ................................................................................................................ 7

SPECIFICATIONS ................................................................................................................................ 9

OPERATIONAL GUIDE ....................................................................................................................... 12

DISPLAYING MODES ......................................................................................................................... 14

SYSTEM SETUP ............................................................................................................................... 17

pH SETUP ...................................................................................................................................... 23

mV SETUP ..................................................................................................................................... 36

ISE SETUP (HI 4522 only) .............................................................................................................. 37

pH CALIBRATION ................................................................................................. 43

pH MEASUREMENT ................................................................................................. 47

mV & RELATIVE mV MEASUREMENTS ................................................................................................ 49

ISE CALIBRATION (HI 4522 only) ........................................................................ 51

ISE MEASUREMENT (HI 4522 only) ........................................................................ 55

CONDUCTIVITY SETUP ......................................................................................................................... 64

RESISTIVITY SETUP ............................................................................................................................. 79

TDS SETUP .............................................................................................................. 81

SALINITY SETUP .............................................................................................................. 83

CONDUCTIVITY CALIBRATION.................................................................................. 85

CONDUCTIVITY MEASUREMENT.................................................................................. 87

RESISTIVITY MEASUREMENT .................................................................................. 90

TDS MEASUREMENT ............................................................................................... 91

SALINITY CALIBRATION .................................................................................. 92

SALINITY MEASUREMENT .................................................................................. 92

TEMPERATURE CALIBRATION .................................................................................. 94

LOGGING ....................................................................................................................................... 95

PC INTERFACE .................................................................................................................................... 100

pH BUFFER TEMPERATURE DEPENDENCE .......................................................................................... 101

PROBE CONDITIONING & MAINTENANCE ..................................................................................... 102

ELECTRODE CONDITIONING & MAINTENANCE ..................................................................................... 103

TROUBLESHOOTING GUIDE ..............................................................................................................105

TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ...................................................................... 107

ACCESSORIES .................................................................................................................................. 108

FC 200B

Plastic-body, open junction, conic, Viscolene, non-refillable, combination pH electrode. Use: meat & cheese.

FC 210B

Glass-body, double junction, conic, Viscolene, non-refillable, combination pH electrode. Use: milk, yogurt.

FC 220B

Glass-body, triple-ceramic, single junction, refillable, combination pH electrode. Use: food processing.

FC 911B

Plastic-body, double junction, refillable with built-in amplifier, combination pH electrode. Use: very high humidity.

113

HI 2031B

Glass-body, semimicro, conic, refillable, combination pH electrode. Use: semisolid products.

HI 1332B

Plastic-body, double junction, refillable, combination pH electrode. Use: general purpose.

PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION

PRELIMINARY EXAMINATION

PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION

Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any damage, notify your dealer. The meters are supplied complete with:

• HI 1131B Glass-body Combination pH Electrode

• HI 76312 Four-ring Conductivity Probe with built-in temperature sensor and ID

• HI 7662-T Temperature probe

• pH 4.01 & 7.01 Buffer solutions, 20 mL each

• HI 700661 Cleaning solution, 2 x 20 mL

• HI 7071S Electrolyte solution

• HI 76404N Electrode Holder

• 12 Vdc Power Adapter

• Instruction Manual

HI 4521 and HI 4522 are supplied with 12 Vdc/230 Vac adapter. HI 4521-01 and HI 4522-01 are supplied with 12 Vdc/115 Vac adapter.

HI 1413B

Glass-body, single junction, flat tip, Viscolene, non-refillable, combination pH electrode. Use: surface measurement.

FC 100B

Plastic-body, double junction, refillable, combination pH electrode. Use: general purpose for food industry.

112

Note: Save all packing material until you are sure that the instrument works properly. Any defective item

must be returned in the original packing with the supplied accessories.

GENERAL DESCRIPTIONGENERAL DESCRIPTION

GENERAL DESCRIPTION

GENERAL DESCRIPTIONGENERAL DESCRIPTION

HI 4521 and HI 4522 are professional bench meters with color graphic LCD for pH, ORP (Oxidation Reduction Potential), ISE (HI 4522 only), conductivity, resistivity, TDS, salinity and temperature measurements. The display can be configured as a single channel or dual channel display in various modes: Basic information only, GLP information, Graph and Log History mode. The main features of the instruments are:

• Two input channels;

• Eight measurement parameters: pH, mV, ISE (HI 4522 only), conductivity, resistivity, TDS, salinity and

temperature;

• Manual selection, automatic and semiautomatic pH calibration in up to five points, with standard (1.68,

3.00, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45) and custom buffers (up to 5 custom buffers);

• Manual Selection and Custom Standard ISE calibration in up to five points, with standard (up to five standard solutions for each measurement unit) and custom solutions (up to 5 custom solutions), with or without temperature compensation (HI 4522 only);

• Pure water checking using the USP <645> standard;

• Conductivity probe automatic recognition;

• Automatic or custom standard conductivity calibration in up to four points, probe offset calibration;

• One fixed point salinity calibration (Percent Scale only);

• AutoHold feature to freeze first stable reading on the LCD;

• Two selectable alarm limits (for pH, mV, ISE, conductivity, resistivity, TDS, salinity);

• Three selectable logging modes: Automatic, Manual, AutoHold logging;

• Up to 100 logging lots for automatic or manual modes and up to 200 USP reports, up to 100 ISE methods reports;

• Selectable area and sampling period feature for automatic logging;

• GLP feature;

• Online and offline graph;

• User-friendly interface on large color graphic LCD (240 x 320 pixels);

• Opto-isolated PC interface via RS232, respectively USB.

HI 1131B

Glass-body, single junction, refillable, combination pH electrode. Use: general purpose.

HI 1330B

Glass-body, semimicro, single junction, refillable, combination pH electrode. Use: laboratory, vials.

HI 1331B

Glass-body, semimicro, single junction, refillable, combination pH electrode. Use: flasks.

HI 1230B

Plastic-body, double junction, gel-filled, combination pH electrode. Use: general, field.

111

pH ELECTRODES

All electrodes part numbers ending in B are supplied with a BNC connector and 1 m (3.3') cable, as shown below:

HI 1043B

Glass-body, double junction, refillable, combination pH electrode. Use: strong acid/alkali.

HI 1053B

Glass-body, triple ceramic, conic shape, refillable, combination pH electrode. Use: emulsions.

FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION

FUNCTIONAL DESCRIPTION

FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION

HI 4521/4522 DESCRIPTION

FRONT PANEL

REAR PANEL

HI 1083B

Glass-body, micro, Viscolene, non-refillable, combination pH electrode. Use: biotechnology, micro titration.

110

1) Liquid Crystal Display (LCD)

2) Main Keyboard

3) USB connector

4) ON/OFF switch

5) Power adapter socket

6) RS232 serial communication connector

7) Conductivity probe connector

8) Temperature probe socket

9) BNC electrode connector for pH/ORP/Ion measurements

10) Reference input socket

KEYBOARD DESCRIPTION

FUNCTION KEYS

To enter/exit calibration mode;

To select the desired measurement mode, pH, mV, Rel mV, ISE (HI 4522 only), Conductivity, Resistivity, TDS, Salinity;

HI 7035M 111800 µS/cm (µmho/cm), 230 mL bottle HI 7035L 111800 µS/cm (µmho/cm), 500 mL bottle HI 8035L 111800 µS/cm (µmho/cm), 500 mL FDA approved bottle HI 7037L 100% NaCl sea water standard solution, 500 mL

ELECTRODE STORAGE SOLUTIONS

To enter Setup (System Setup, pH Setup, mV Setup, ISE Setup (HI 4522 only), Conductivity Setup, Resistivity Setup , TDS Setup or Salinity Setup) and to access Log Recall function;

To obtain general information about the selected option / operation.

VIRTUAL KEYS

The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow you to

perform the displayed function, depending on the current menu (e.g.

Measure

mode).

Note: All the virtual keys are assigned to the focused channel (with

LCD GENERAL DESCRIPTION

and in

key exception).

HI 70300L Storage Solution, 500 mL bottle HI 80300L Storage Solution in FDA approved bottle, 500 mL

ELECTRODE AND PROBE CLEANING SOLUTIONS

HI 70000P Electrode Rinse Sachets, 20 mL, 25 pcs HI 7061L General Purpose Solution, 500 mL bottle HI 7073L Protein Cleaning Solution, 500 mL bottle HI 7074L Inorganic Cleaning Solution, 500 mL bottle HI 7077L Oil & Fat Cleaning Solution, 500 mL bottle HI 8061L General Purpose Solution in FDA approved bottle, 500 mL HI 8073L Protein Cleaning Solution in FDA approved bottle, 500 mL HI 8077L Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL

ELECTRODE REFILL ELECTROLYTE SOLUTIONS

HI 7071 3.5M KCl + AgCl Electrolyte, 4x30 mL, for single junction electrodes HI 7072 1M KNO3 Electrolyte, 4x30 mL HI 7082 3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes HI 8071 3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL, for single junction electrodes HI 8072 1M KNO3 Electrolyte in FDA approved bottle, 4x30 mL HI 8082 3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction electrodes HI 8093 1M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL

ORP PRETREATMENT SOLUTIONS

HI 7020L Test Solution 200-275 mV, 500 mL bottle HI 7021L Test Solution 240 mV, 500 mL bottle HI 7022L Test Solution 470 mV, 500 mL bottle

HI 7091L Reducing Pretreatment Solution, 500 mL HI 7092L Oxidizing Pretreatment Solution, 500 mL

109

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ACCESSORIESACCESSORIES

ACCESSORIES

ACCESSORIESACCESSORIES

pH BUFFER SOLUTIONS

HI 6001 pH 1.679 Buffer Solution, 500 mL bottle HI 6003 pH 3.000 Buffer Solution, 500 mL bottle HI 8004L pH 4.01 Buffer Solution in FDA approved bottle, 500 mL HI 6004 pH 4.010 Buffer Solution, 500 mL bottle HI 8006L pH 6.86 Buffer Solution in FDA approved bottle, 500 mL HI 6068 pH 6.862 Buffer Solution, 500 mL bottle HI 8007L pH 7.01 Buffer Solution in FDA approved bottle, 500 mL HI 6007 pH 7.010 Buffer Solution, 500 mL bottle HI 6091 pH 9.177 Buffer Solution, 500 mL bottle HI 8009L pH 9.18 Buffer Solution in FDA approved bottle, 500 mL HI 8010L pH 10.01 Buffer Solution in FDA approved bottle, 500 mL HI 6010 pH 10.010 Buffer Solution, 500 mL bottle HI 6124 pH 12.450 Buffer Solution, 500 mL bottle

CONDUCTIVITY BUFFER SOLUTIONS

HI 70033P 84 µS/cm (µmho/cm), 20 mL sachets (25 pcs.) HI 7033M 84 µS/cm (µmho/cm), 230 mL bottle HI 7033L 84 µS/cm (µmho/cm), 500 mL bottle HI 8033L 84 µS/cm (µmho/cm), 500 mL FDA approved bottle HI 70031P 1413 µS/cm (µmho/cm), 20 mL sachets (25 pcs.) HI 7031M 1413 µS/cm (µmho/cm), 230 mL bottle HI 7031L 1413 µS/cm (µmho/cm), 500 mL bottle HI 8031L 1413 µS/cm (µmho/cm), 500 mL FDA approved bottle HI 70039P 5000 µS/cm (µmho/cm), 20 mL sachets (25 pcs.) HI 7039M 5000 µS/cm (µmho/cm), 230 mL bottle HI 7039L 5000 µS/cm (µmho/cm), 500 mL bottle HI 8039L 5000 µS/cm (µmho/cm), 500 mL FDA approved bottle HI 70030P 12880 µS/cm (µmho/cm), 20 mL sachets (25 pcs.) HI 7030M 12880 µS/cm (µmho/cm), 230 mL bottle HI 7030L 12880 µS/cm (µmho/cm), 500 mL bottle HI 8030L 12880 µS/cm (µmho/cm), 500 mL FDA approved bottle HI 7034M 80000 µS/cm (µmho/cm), 230 mL bottle HI 7034L 80000 µS/cm (µmho/cm), 500 mL bottle HI 8034L 80000 µS/cm (µmho/cm), 500 mL FDA approved bottle

108

SPECIFICATIONSSPECIFICATIONS

SPECIFICATIONS

SPECIFICATIONSSPECIFICATIONS

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TEMPERATURE CORRELATION

TEMPERATURE CORRELATIONTEMPERATURE CORRELATION

FOR FOR

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FOR FOR

H SENSITIVE GLASSH SENSITIVE GLASS

H SENSITIVE GLASS

H SENSITIVE GLASSH SENSITIVE GLASS

The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher. In addition, the response time will suffer to a greater degree at temperatures below 25 ºC (77 ºF).

Since the resistance of the pH electrode is in the range of 50 – 200 Mohm, the current across the membrane is in the pico Ampere range. Large currents can disturb the calibration of the electrode for many hours.

For these reasons high humidity environments, short circuits and static discharges are detrimental to a stable pH reading.

The pH electrode’s life also depends on the temperature. If constantly used at high temperatures, the electrode life is drastically reduced.

Typical Electrode Life

Ambient Temperature 1 – 3 years 90 ºC (194 ºF) Less than 4 months 120 ºC (248 ºF) Less than 1 month

Alkaline Error

High concentrations of sodium ions interfere with readings in alkaline solutions. The pH at which the interference starts to be significant depends upon the composition of the glass. This interference is called alkaline error and causes the pH to be underestimated. Hanna’s glass formulations have the indicated characteristics.

Sodium Ion Correction for the Glass at 20-25 ºC (68-77 ºF) Concentration pH Error

0.1 Mol L-1 Na

1.0 Mol L-1 Na

13.00

13.50

14.00

12.50

13.00

13.50

14.00

0.10

0.14

0.20

0.10

0.18

0.29

0.40

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OPERATIONAL GUIDE

OPERATIONAL GUIDEOPERATIONAL GUIDE

TROUBLESHOOTING GUIDETROUBLESHOOTING GUIDE

TROUBLESHOOTING GUIDE

TROUBLESHOOTING GUIDETROUBLESHOOTING GUIDE

POWER CONNECTION

Plug the 12 VDC adapter into the power supply socket. Note: These instruments use non volatile memory to retain the meter settings, even when unplugged.

ELECTRODE AND PROBE CONNECTIONS

For pH or ORP measurements connect a pH / ORP electrode with internal reference to the BNC connector located on the rear panel of the instrument.

For ISE measurements (HI 4522) connect, an ISE electrode with internal reference to the BNC connector located on the rear panel of the instrument.

For electrodes with a separate reference connect the electrode’s BNC to the BNC connector and the electrode’s reference to the reference input socket.

For temperature measurements and automatic temperature compensation connect the temperature probe to the appropriate socket (mV channel only).

For conductivity, resistivity, TDS or salinity measurements connect a conductivity probe to the DIN connector located on the rear panel of the instrument.

INSTRUMENT START UP

• Turn the instrument on from the power switch located on the rear panel of the instrument.

• Please wait until the instrument finishes the initialization process.

Note: It is normal for the loading process to take a few seconds. If the instrument doesn’t display the next

screen, restart the meter using the power switch. If the problem persists, contact your dealer.

CONDUCTIVITY / RESISTIVITY / TDS / SALINITY CHANNEL

105

For AmpHelFor AmpHel

For AmpHel

For AmpHelFor AmpHel

®®

electrodes: electrodes:

electrodes:

electrodes: electrodes:

CHANNEL SELECTION

If the electrode does not respond to pH changes, the battery run down and the electrode should be replaced.

MEASURE

Rinse the pH electrode tip with distilled water. Immerse the tip (bottom 4 cm /1½”) in the sample and stir gently for a few seconds.

For a faster response and to avoid cross-contamination of the samples, rinse the electrode tip with a few drops of the solution to be tested, before taking measurements.

STORAGE PROCEDURE

To minimize clogging and assure a quick response time, the glass bulb and the junction of pH electrode should be kept moist and not allowed to dry out.

Replace the solution in the protective cap with a few drops of HI 70300 or HI 80300 Storage Solution or, in its absence, Filling Solution (HI 7071 or HI 8071 for single junction and HI 7082 or HI 8082 for double junction electrodes). Follow the Preparation Procedure on page 103 before taking measurements.

Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.

PERIODIC MAINTENANCE

Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the electrode. Rinse off any salt deposits with water.

pH Probe Maintenance

• Press while in

Measure

mode to access channel

selection menu. Four available options will be displayed: Channel 1, Channel 2, or multi-channel with the first or the second channel focused. The “Choose Channel Configuration” message is displayed in the Reminder messages area

when

is pressed.

• Select the desired option by pressing the appropriate key: , , or . The instru-

ment will enter in the selected option

Measure

mode.

For refillable electrodes:For refillable electrodes:

For refillable electrodes:

For refillable electrodes:For refillable electrodes:

Refill the reference chamber with fresh electrolyte (HI 7071 or HI 8071 for single junction or HI 7082 or HI 8082 for double junction electrodes). Allow the electrode to stand upright for 1 hour. Follow the Storage

Procedure above.

pH CLEANING PROCEDURE

General

•

Protein

•

Inorganic

Oil/grease

•

Soak in Hanna HI 7061 or HI 8061 General Cleaning Solution for approximately ½ hour. Soak in Hanna HI 7073 or HI 8073 Protein Cleaning Solution for 15 minutes. Soak in Hanna HI 7074 Inorganic Cleaning Solution for 15 minutes. Rinse with Hanna HI 7077 or HI 8077 Oil and Fat Cleaning Solution.

IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled electrodes) and soak the electrode in HI 70300 or HI 80300 Storage Solution for at least 1 hour before taking measurements.

104

DISPLAYING MODESDISPLAYING MODES

DISPLAYING MODES

DISPLAYING MODESDISPLAYING MODES

For each measurement mode (pH, mV, Rel mV, Ion, Conductivity, Resistivity, TDS or Salinity) the following display configurations are available: Basic, Good Laboratory Practice (GLP), Graph and Log History.

Basic

Accessing this option, the measured value and its units are displayed on the LCD, along with the temperature value, temperature compensation mode, and minimal GLP data.

To choose the Basic displaying mode:

• Press Configuration” message will be displayed in the Reminder

messages area.

• Press mation for the selected measurement mode.

GLP

Accessing this option, a detailed GLP data will be displayed on the LCD for pH / ISE: Last Calibration Date and Time, Offset and Slope values, Calibration Buffers, Electrode Condition and for conductivity / salinity: Last Calibration Date and Time, Calibration Standards, Cell Constant, Probe Offset, Reference Temperature, Compensation Coefficient, Temperature Compensation.

Note: If only a one-point pH calibration is performed or the current

calibration does not include at least two consecutive standard buffers of pH 4.01, 7.01 (6.86) and 10.01 (9.18) buffers, the Electrode Condition will be unknown.

To access the GLP displaying mode:

• Press while in Configuration” message will be displayed in the Reminder

messages area.

• Press data.

while in

. The instrument will display the basic infor-

. The instrument will display the detailed GLP

Measure

mode. The “Choose Display

Measure

mode. The “Choose Display

ELECTRODE CONDITIONINGELECTRODE CONDITIONING

ELECTRODE CONDITIONING

ELECTRODE CONDITIONINGELECTRODE CONDITIONING

& MAINTENANCE& MAINTENANCE

& MAINTENANCE

& MAINTENANCE& MAINTENANCE

PREPARATION PROCEDURE

Remove the protective cap of the pH electrode. DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear

when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode.

These bubbles can be removed by “shaking down” the electrode as you would do with a glass thermometer. If the bulb and/or junction is dry, soak the electrode in HI 70300 or HI 80300

For refillable electrodes:For refillable electrodes:

For refillable electrodes:

For refillable electrodes:For refillable electrodes:

If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI 7082 or HI 8082 3.5M KCl Electrolyte Solution for double junction or HI 7071 or HI 8071 3.5M KCl+AgCl Electrolyte Solution for single junction electrodes.

For faster response, unscrew the fill hole screw during measurements.

Storage Solution for at least one hour.

103

PROBE CONDITIONING & MAINTENANCEPROBE CONDITIONING & MAINTENANCE

PROBE CONDITIONING & MAINTENANCE

PROBE CONDITIONING & MAINTENANCEPROBE CONDITIONING & MAINTENANCE

MEASURE

Rinse the conductivity probe with distilled water. Immerse the tip (bottom 4 cm /1½”) in the sample and stir gently for a few seconds.

For a faster response and to avoid cross-contamination of the samples, rinse the probe with a few drops of the solution to be tested, before taking measurements.

PERIODIC MAINTENANCE

Inspect the probe and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable. Connectors must be perfectly clean and dry. Rinse off any salt deposits with water.

If more cleaning is required, remove the probe sleeve and clean the probe with a cloth or a nonabrasive detergent. Make sure to reinsert the sleeve onto the probe properly and in the right direction. After cleaning the probe, recalibrate the instrument.

The platinum rings are sustained with glass. Take great care while handling the probe.

Graph

Accessing this option, the online graph with the currently logged values (pH, mV, Rel mV, ISE respectively Conductivity, Resistivity, TDS, Salinity vs. Seconds) will be displayed.

If there is no active log, the previously logged data for the selected parameter will be plotted.

Notes: • If no data were logged, the graph displaying mode

will not be accessible.

• If no automatic log is saved, the offline graph will not be available.

To access the offline / online graph:

• Press Configuration” message will be displayed in the Reminder

messages area.

• Press

When the online graph is displayed:

• Use and to move the graph along horizontal (

while in

Measure

mode. The “Choose Display

Time

) axis.

IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water.

102

• Press to access the zoom menu for the vertical ( zooming vertical axis.

• Press to return to the main menu.

When the offline graph is displayed:

• Use the arrow keys to move the graph along the horizontal and vertical axes.

• Press to access the zoom menu for horizontal and vertical axes. Use , or /

/ / respectively / / / to switch

between the active zooming axes. Press or to zoom the selected axis.

Note: While in zoom graph menu the key is not accessible.

• Press to return to the main menu.

Parameter

) axis. Use or for

Log History

PMET SREFFUBHp

Cº Kº Fº 976.1 000.3 010.4 268.6 010.7 771.9 010.01 454.21

0 372 23 076.1 270.3 700.4 289.6 031.7 954.9 613.01 973.31

5 872 14 076.1 150.3 200.4 949.6 890.7 193.9 542.01 871.31

01 382 05 176.1 330.3 000.4 129.6 070.7 823.9 081.01 589.21

51 882 95 376.1 910.3 100.4 798.6 640.7 372.9 811.01 997.21

02 392 86 576.1 800.3 400.4 878.6 720.7 222.9 260.01 126.21

52 892 77 976.1 000.3 010.4 268.6 010.7 771.9 010.01 054.21

03 303 68 386.1 599.2 710.4 158.6 899.6 731.9 269.9 682.21

53 803 59 886.1 199.2 620.4 248.6 989.6 801.9 919.9 821.21

04 313 401 396.1 099.2 730.4 738.6 389.6 960.9 188.9 879.11

54 813 311 007.1 099.2 940.4 438.6 979.6 040.9 748.9 438.11

05 323 221 707.1 199.2 260.4 438.6 879.6 410.9 718.9 796.11

55 823 131 517.1 399.2 670.4 638.6 979.6 099.8 397.9 665.11

06 333 041 427.1 599.2 190.4 938.6 289.6 969.8 377.9 244.11

56 833 941 437.1 899.2 701.4 448.6 789.6 849.8 757.9 323.11

07 343 851 447.1 000.3 321.4 058.6 399.6 929.8 647.9 112.11

57 843 761 557.1 200.3 931.4 758.6 100.7 019.8 047.9 401.11

08 353 671 767.1 300.3 651.4 568.6 010.7 198.8 837.9 300.11

58 853 581 087.1 200.3 271.4 378.6 910.7 178.8 047.9 809.01

09 363 491 397.1 000.3 781.4 088.6 920.7 158.8 847.9 918.01

59 863 302 708.1 699.2 202.4 888.6 040.7 928.8 957.9 437.01

Accessing this option, last logged records will be displayed on the LCD. The log history list also contains the appropriate main parameter values, the logged temperature, the temperature compensation source / mode, as well as the records time stamp.

To access the Log History displaying mode:

• Press

while in

Measure

mode. The “Choose Display

Configuration” message will be displayed in the Reminder messages area.

• Press . The instrument will display the log history regarding the selected measure mode.

Notes: • When an alarm condition is active, all logged records will have an exclamation mark (!).

• When a meter is in auto-hold, the logged records will have an “H” symbol.

• If another measure mode is selected, the Log History will be cleared.

• If the temperature unit is changed, all logged temperature values will be automatically displayed in the new temperature unit.

H BUFFER TEMPERATUREH BUFFER TEMPERATURE

H BUFFER TEMPERATURE

H BUFFER TEMPERATUREH BUFFER TEMPERATURE

DEPENDENCEDEPENDENCE

DEPENDENCE

DEPENDENCEDEPENDENCE

Temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lower degree than normal solutions.

During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature.

During calibration the instrument will display the pH buffer value at 25 ºC.

101

PC INTERFACEPC INTERFACE

PC INTERFACE

PC INTERFACEPC INTERFACE

SYSTEM SETUPSYSTEM SETUP

SYSTEM SETUP

SYSTEM SETUPSYSTEM SETUP

Data transmission from the instrument to the PC can be done with the HI 92000 Windows® compatible software (optional). HI 92000 also offers graphing and on-line help feature.

Data can be exported to the most popular spreadsheet programs for further analysis. HI 4521 and HI 4522 instruments have two available serial interfaces: RS232 and USB. The desired serial

interface can be selected from the settings window of the HI 92000 software. If choosing the RS232 serial interface, use the optional Hanna HI 920010 cable connector to connect your

instrument to a PC. Make sure that your instrument is switched off and then plug one connector to the instrument RS232 socket and the other one to the serial port of your PC.

Note: Other cables than HI 920010 may use a different configuration. In this case, communication between

instrument and PC may not be possible.

If choosing the USB serial interface, use a standard USB cable to connect your instrument to the PC. For both serial interfaces, make sure that the instrument and the HI 92000 software have the same baud rate

and the appropriate communication port.

The System Setup menu allows the user to customize the user interface, consult the meter information, set the external serial communication interface and to restore the manufacturer settings.

Accessing System Setup

• Press

• Press . The system setup options will be displayed on the LCD.

To access a System Setup option:

• Use or to highlight the desired option.

• Press to access the selected option.

The following is a detailed description of the System Setup option screen.

Beeper

This option allows the user to enable or disable the beeper. When the beeper is enabled, a specific beep will be heard when the reading becomes stable, when an alarm condition is reached, a wrong key is pressed.

while in

Measure

mode.

when pressing a key or if

Stability Indicator

When the reading becomes stable, the instrument delivers a medium beep only if this option is ON, along with the “Stable” indicator on the LCD.

Alarm

If this option is ON, a continuous double beep will be heard each time the set limits in exceeded, along with the “Alarm” indicator on the LCD.

Measure

mode are

Key Pressed

If this option is ON, a short beep will be heard each time a valid key is pressed.

Wrong Key

If this option is ON, a long beep will be heard when an incorrect key is pressed.

100

To set the Beeper:

• Use

• Press and use or to highlight the desired beeper associated parameter you want to modify.

• Press and use or to highlight the beeper status option.

• Press to confirm your selection and return to the Beeper menu or press

or to select the Beeper option.

to return without changing.

highlighted lot. The “Please wait...” message will be displayed on the LCD for a short period. The user customised report will be displayed on the LCD.

Note: For automatic logging only, it is possible to view the

plotted graph.

• Press to display the graph.

• By pressing it is possible to move the graph along the horizontal or vertical axis with the arrow keys.

• If pressing while the graph is displayed, the zoom menu for the horizontal and vertical axes will be accessed. Press

, or / /

Saving Confirmation

When enabling this option, a prompt will appear on the LCD alerting the user to save the modified values by pressing

without saving by pressing or canceling the saving operation and return to the editing mode by pressing . If disabled, the

modified values will be saved automatically. To enable / disable the saving confirmation:

• Use or to select the

• Press and use or to choose enabled / disabled.

• Press to confirm your selection or press cancel operation.

GLP Data

This option allows the user to set general information which will appear in the log reports. The options can have a max of 10 characters.

Operator ID Instrument ID Company Name

– this option allows you to edit the name of the operator.

– this option allows you to edit an identification name/number for the instrument.

– this option allows you to edit the company name.

Additional Info 1 & Additional Info 2

Saving Confirmation

– for general purpose notations.

, exiting

option.

/ / / / / to switch between the active

zooming axes and then zoom in or out on the selected axis by pressing the appropriate virtual key.

• Press to return to the previous menu at any time.

To delete lots:

• Press while in

• Press or to access delete or delete all mode. Otherwise, press to return to

• After selecting one of the deleting modes, use or

to select one lot and then press or to delete the selected lot or all lots. The “Please

wait...” message will be displayed on the LCD until the selected lot or all lots are deleted.

• Press and then press to exit deleting mode and return to

Log Recall

• Press

Note: Logged lots should also be deleted whenever “Please Delete

Old Log Files” or “Low Data Logging Space” message appears on the LCD, in the Reminder messages area.

to exit

view mode.

Log Recall

mode.

mode and return to

Log Recall

view mode.

Measure

mode.

set as Direct/AutoHold in order to use this logging mode.

To log data using this mode:

• Press while in frozen on the LCD, after was pressed and the stability criterion reached.

• To store another frozen value, press to return to normal logging mode and then again.

• To stop the logging session, press or / .

Notes: • For the automatic logging, if the maximum logging time (24h) has been reached, a warning pop-

up will be displayed on the LCD in order to stop the current log and start another one in a new lot.

• If 100 lots have been saved or maximum 10000 records have been manually stored, a warning pop-up will be displayed on the LCD in order to delete one lot or to select a new lot for the manual logging to log other records.

LOG RECALL

This feature allows the user to view all stored data. If no data were logged, the “No records were found” message will be displayed on the LCD in the Log Recall screen. Otherwise, the instrument will display all the memorized lots in accordance with the selected option: Automatic Log, Manual Log ISE Method Report (HI 4522), or USP Report.

To view the memorized data:

• Press

• Press . The “Choose Log Report Type” message will be displayed in the Reminder messages area.

• Press , , the desired Log / ISE/ USP Report type. All logged lots for the

selected Log Report type will be displayed on the LCD.

• To filter the displayed lots, press and then the desired unit ( , ,

lected measurement unit lots will be displayed on the LCD.

• Select the desired lot with

while in

, ,

to display the logged / report data from the

Measure

mode to start the logging session. The logged values are only the ones

Measure

mode.

or ). Only the se-

or and press

to select

(HI 4522),

To set the GLP Data:

• Use

• Press and use or to highlight the desired option.

• Press to edit the desired information. The Text Editor menu will be displayed on the LCD.

• Enter the desired information by using and to highlight the desired character. It is also possible to delete the last

character by positioning the cursor on the Backspace character ( ) and pressing .

• Press to return to the

Confirmation

option, to escape without saving or to return to the editing mode. Otherwise, the modified options are saved

automatically.

Date & Time

This option allows the user to set the current date & time and the format in which they appear.

or to select the

is enabled, press to accept the modified

GLP Data

option.

menu. If the

Saving

Set Date and Time

This option allows you to set the current date (year/month/day) and time (hour/minute/second). Notes: • Only years starting with 2000 are accepted.

• The time is set using the selected time format. For 12 Hour time format only, the AM/PM can also be selected with or .

Set Time Format

This option allows you to choose between 12 Hour (AM/PM) time format and 24 Hour time format from the displayed pop-up menu.

Set Date Format

This option allows you to choose the desired date format from 6 available options: DD/MM/YYYY; MM/DD/YYYY; YYYY/MM/DD; Mon DD, YYYY; DD-MM-YYYY and YYYY-Mon-DD.

To set the Date & Time:

• Use

• Press and use or to highlight the desired option you want to modify.

or to select the

Date& Time

option.

• Press to confirm your selection. Use and and then use to modify the value with or (for

other two options press to confirm your selection and select one of the displayed formats with or .

• Press to confirm your selection and return to the

options.

& Time

• Press to return to the previous mode.

Notes: • For the

LCD Setup

This option allows the user to set the parameter can be adjusted within 7 steps, while the be set from 1 to 60 minutes or it can be OFF (disabled). All the changes are visible on the LCD for each parameter.

Note: If the instrument backlight is turned off after the set period of

To set the LCD Setup:

• Use or to select the

• Press and use key to highlight the desired

parameter.

• Use

• Press

Set Date and Time

enabled, press to accept the modified option, to escape without saving or to return to the editing mode.

Otherwise, the modified option is saved automatically.

• If the time is changed with more than one hour before last pH/ION calibration, a pop-up warning will appear on the LCD, notifying the user that a date/time conflict has occured and some time-dependent modes could work improperly (e.g.

Log

time, press any key to turn it back on.

or to adjust the selected parameter.

to return to the

Set Date and Time

option, if the

Contrast

Saving Confirmation

, the

LCD Setup

System Setup

option). For the

Date

Backlight Backlight

menu with saving.

of the LCD and the

parameter within 4 steps. The

option.

Backlight Saver

Measure, GLP

. The

Contrast

can

The “Logging”, sampling period and “AutoHold” indicators will be displayed on the LCD.

• To store another frozen value, press

• To stop the logging session, press / .

LOGGING MODE 3

This logging mode can be used for any sample measurements. By choosing this logging mode, / able in

Measure

mode.

To log data using this mode:

• Press while in record. The “Logged” indicator will be displayed on the LCD.

• The records will be stored in one lot. In order to change the logging lot, see the measured parameter setup for details, Log option, New Lot generation.

LOGGING MODE 4

This logging mode can be used for multiple samples measurement. By choosing this logging mode, / To log data using this mode:

• Press /

• To store another frozen value, press to return to normal logging mode and then again.

• The records will be stored in one lot. In order to change the logging lot, see the measured unit

LOGGING MODE 5

This logging mode can be used for multiple samples measurement. By choosing this logging mode, and will be available in

Notes: • The

and / will be available in

when the key was pressed. When the measured value is frozen on the LCD by pressing / and the stability criterion is reached, the logged value is the one that has been frozen on the LCD.

details, Log option, New Lot generation.

mode, depending on the focused channel.

• If the Reading Mode option is set as Direct and the Logging Mode 5 session is started, a warning pop-up will be displayed on the LCD, informing the user that the Reading Mode option must be

Measure

mode to manually log a

while in

Measure

or and or will be available in multichannel

/ again.

will be avail-

Measure

mode.

mode to manually log a record. Each value is logged at the time

mode.

Setup

Measure

for

• To stop the logging session, press / . The Log Save screen will display thelog lot ID, the settable log

interval / sampling:

• Press to adjust the log interval and/or the log sampling or press to save the current log in

the displayed format.

• Press to enter log interval edit menu and use

or to adjust the logging start-stop time or the log sampling. Press to save the current value and use or to adjust

next / previous parameter.

• Press press to save the current log.

• While the instrument is saving the data, a “Please wait...” pop-up message will be displayed on the LCD.

LOGGING MODE 2

This logging mode can be used for multiple samples measurement. By choosing this logging mode, / and

to exit log interval edit menu and then

Language

This option allows the user to choose the desired language in which all information will be displayed.

To select the Language:

• Use

• Press and use or to highlight the desired language.

• Press to confirm your selection and return to the

Setup

menu without changing.

Serial Communication

This option allows the user to set the desired speed for the serial communication (baud rate) between the instrument and PC from

1200, 2400, 4800 or 9600

To set the serial communication:

• Use or to select the option.

• Press and use or to highlight the desired baud rate.

• Press to confirm your selection and return to the

Setup

or to select the Language option.

menu or press

to return to the System Setup

to return without changing.

System

Serial Communication

System

/ will be available in

To log data using this mode:

• Press / while in logging session. When the measured value is frozen on the LCD by pressing reached, the logged value is the one that has been frozen on the LCD until returning to normal logging mode by pressing .

/ and the stability criterion is

Measure

mode.

mode to start the

Note: The meter and the PC program must have the same baud rate.

Meter Information

edoMgniggoL epyTgniggoL edoMgnidaeR

1 citamotuA tceriD

2 citamotuA dloHotuA/tceriD

3 launaM tceriD

4 launaM dloHotuA/tceriD

5 dloHotuA dloHotuA/tceriD

This option provides general information about the instrument serial number (each instrument has an unique identification serial number), the software version and the factory calibration date and time (for mV, conductivity and temperature).

Note: All the instruments are factory calibrated for mV, conductivity

and temperature. After one year following factory calibration, the “Factory Calibration Due” message will appear on the LCD, in the Reminder messages area, notifying the user that the instrument should be taken to the nearest Hanna Customer Service for factory calibration.

To view the meter information:

• Use

or to select the

Meter Information

option.

LOGGINGLOGGING

LOGGING

LOGGINGLOGGING

This feature allows the user to log pH, mV, ISE (HI 4522), conductivity, resistivity, TDS, salinity and temperature. The logging behaviour is dependent on the

Logging Type

and

Reading Mode

options from the

parameter setup. The

Logging Data Configuration

options from the appropriate unit setup must be set first in order to be saved

into the log report. The maximum number of logged records is 10.000/lot, the maximum logging time is 24h and up to 100 lots

can be saved. Regarding data logging, the available logging modes are shown in the table below:

• Press to confirm and to view the meter information or press to return to the menu.

Restore Factory Settings

This option allows the user to reset the instrument to the default factory settings.

To restore the factory settings:

• Use or to select the

Restore Factory Settings

option.

• Press to confirm your selection. A pop-up box will be displayed, asking for confirmation.

• Press to confirm your selection and return to the

or press to return without restoring defaults.

Setup

System

System Setup

LOGGING MODE 1

This logging mode can be used to monitor a chemical reaction. By choosing this logging mode, be available in

Measure

mode.

To log data using this mode:

• Press / while in

Measure

mode to start the

logging session. The “Logging” and the Sampling Period indicators will be displayed on the LCD and data will be stored at the set sampling period.

Note: While automatic logging is running, the measured pa-

rameter setup is not available. A warning message will be displayed if the setup is accessed.

• If accessing Graph option while logging, the online graph can be visualized on the LCD (see

Display Mode

section).

• If accessing Log History option while logging, last logged data can be visualized on the LCD (see

Display Mode

will

TEMPERATURE CALIBRATIONTEMPERATURE CALIBRATION

TEMPERATURE CALIBRATION

TEMPERATURE CALIBRATIONTEMPERATURE CALIBRATION

pHpH

SETUP SETUP

SETUP

pHpH

SETUP SETUP

The temperature user calibration menu can be accessed at the meter startup by pressing simultaniously three keys as in the below drawing:

Note: The temperature user calibration is performed in three points: around 0°C, 50°C, 100°C.

To perform temperature user calibration:

• Select the desired temperature channel by pressing (the temperature channel is switched between temperature EC

channel and temperature pH channel).

• Press to start the temperature calibration. Adjust the temperature preset value using or when

necessary.

• Insert the probe in the beaker with water at 0°C.

• Wait to stabilize and then press to confirm the calibration point.

• Repeat the previous three steps for 50°C and 100°C.

• Save the calibration.

• Press to return to measure mode.

The pH Setup menu allows the user to set the parameters associated with pH measurement and calibration.

Accessing pH Setup

• Press while in select pH range for the desired channel.

• Press and then to access pH Setup menu.

To access a pH Setup option:

• Use or to highlight the desired option.

• Press

The following is a detailed description of the pH Setup option screens.

Profile

Choosing this option the measuring and the calibration mode can be customized. Up to 10 profiles can be defined by the user.

The available options are:

Save Current Profile: Load Profile: Delete Profile:

load from available profiles.

delete a profile.

Measure

mode and then to

to access the selected option.

save the current profile.

Save Current Profile

To save the current profile:

• Use or to select the

Profile

option.

Note: Press

if you want to clear the temperature user calibration.

• Press and then select

Text Editor

box will be displayed on the LCD.

• Enter the desired profile name by using to highlight the desired character and then press to add it to

the text bar. It is also possible to delete the last character by positioning the cursor on the Backspace character (

• Press to return to the Profile options.

Save Current Profile

and

) and pressing .

option. The

Note: The saved profile will automatically become the current profile.

PRACTICAL SALINITY SCALE (UNESCO 1978)PRACTICAL SALINITY SCALE (UNESCO 1978)

PRACTICAL SALINITY SCALE (UNESCO 1978)

PRACTICAL SALINITY SCALE (UNESCO 1978)PRACTICAL SALINITY SCALE (UNESCO 1978)

Load Profile

To load one profile:

• Use or to select the

• Press

Load Profile

• Press

and use or to highlight the

option.

A list with all customised profiles will be displayed

Profile

option.

on the screen.

• Use or to select the desired profile and press

confirm or

to exit without selecting.

Delete Profile

To delete one of the existing profiles:

• Use or to select the

• Press

Delete Profile

• Press

and use or to highlight the

option.

A list with all customised profiles will appear on

Profile

option.

the screen.

• Use or to select the desired profile and press

• Press

to return to the previous menu.

According to the definition, salinity of a sample in psu (practical salinity units) is calculated using the following formula:

where:

RT - coefficient;

CT(sample) - uncompensated conductivity at T °C;

C(35,15)= 42914 µS/cm - the corresponding conductivity of KCl solution containing a mass of 32.4356 g KCl / 1 Kg solution; rT - temperature compensation polynom

a0=0.008 b0=0.0005 a1=-0.1692 b1=-0.0056 a2=25.3851 b2=-0.0066 a3=14.0941 b3=-0.0375 a4=-7.0261 b4=0.0636 a5=2.7081 b5=-0.0144 c0=0.008 c1=0.0005 X=400R Y=100R

f(T)=(T-15)/[1+0.0162(T-15)]

Note: The formula can be applied for salinity values between 0 and 42 psu.

The formula can be applied for temperatures between -2 °C and 35 °C.

SALINITY CALIBRATIONSALINITY CALIBRATION

SALINITY CALIBRATION

SALINITY CALIBRATIONSALINITY CALIBRATION

Salinity calibration is a one-point calibration procedure at 100.0% NaCl. Use the HI 7037L calibra- tion solution (sea water solution) as a 100% NaCl standard solution.

To enter salinity calibration:

• Set the meter for salinity range;

• Select the

• Rinse the probe with some of the calibration solution or deionized water;

• Immerse the probe into HI 7037L solution. The sleeve holes must be completely submerged. Tap

the probe repeatedly to remove any air bubbles that may be trapped inside the sleeve.

• Enter in calibration mode by pressing ;

• Wait to stabilize;

• Press

Three measurement scales are available for salinity (Natural Sea Water Scale, Practical Salinity Scale and Percent Scale).

NATURAL SEA WATER SCALE (UNESCO 1966)NATURAL SEA WATER SCALE (UNESCO 1966)

NATURAL SEA WATER SCALE (UNESCO 1966)

NATURAL SEA WATER SCALE (UNESCO 1966)NATURAL SEA WATER SCALE (UNESCO 1966)

According to the definition, salinity of a sample in ppt is calculated using the following formula:

Percent Scale

to finish salinity calibration or press to cancel calibration.

(see

Salinity Setup

SALINITY MEASURESALINITY MEASURE

SALINITY MEASURE

SALINITY MEASURESALINITY MEASURE

section);

MENTMENT

MENT

MENTMENT

Temperature

The temperature has a direct influence on pH. This option allows the user to choose the temperature source and units, as well as the desired manual temperature for manual temperature compensation mode.

Temperature Source

If using a temperature probe, Automatic Temperature Compensation will be performed relative to the displayed temperature, with the “ATC 1 / 2” indicator displayed on the LCD. The ATC option can be selected for Channel 1 or Channel 2, in accordance with the configurated channel utilizing a temperature probe. If no temperature probe is detected, Manual Temperature Compensation will be performed, with the “MTC” indicator on the LCD.

Temperature Unit

Accessing this option, the desired temperature unit can be chosen (Celsius, Fahrenheit or Kelvin degrees) and the meter will automatically make the conversion for the selected unit.

Manual Temperature

If no temperature probe is connected or the set manually.

To set one of the temperature options:

• Use or to select the

• Press and use or to highlight the desired temperature option you wish to modify.

• Press and use or to highlight the desired option (for

or to adjust the temperature value between

the displayed limits (for

• Press to confirm your selection (for

Temperature Source

Manual Temperature

Temperature Source

Temperature

Unit options

option.

) or use

option).

Temperature Source

is set as

Manual

, the desired temperature can be

where: RT - coefficient;

CT(sample) - uncompensated conductivity at T °C;

C(35,15)= 42914 µS/cm - the corresponding conductivity of KCl solution containing a mass of 32.4356 g KCl / 1 Kg solution;

rT - temperature compensation polynom.

Note: The formula can be applied for temperatures between 10 °C and 31 °C.

Unit

options) or press to save the current value (for

Temperature

Calibration

This option allows the user to set all the data regarding the pH calibration process.

option). Otherwise, press

to cancel operation.

Manual

Buffer Entry Type

Three entry modes for the pH buffers used for calibration are available: Automatic – the instrument automatically selects the closest buffer to the measured pH value from the edited

buffer group. Semiautomatic – the instrument automatically selects the closest buffers to the measured pH value from all

available buffers and you can choose the one used. Manual Selection – the desired pH buffer is manually selected from all available buffers, regardless of measured value.

To select the Buffer Entry Type:

• Use

or to select the Calibration option.

TDS MEASURETDS MEASURE

TDS MEASURE

TDS MEASURETDS MEASURE

Make sure the TDS factor has been set before taking TDS measurements (see

MENTMENT

MENT

MENTMENT

TDS Setup

section).

• Press and use or to highlight the

Buffer Entry Type

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

option.

1st Cal. Point

Two options are available for the 1st Cal.: Point and Offset. If Point option is selected, the slope values adjacent to the calibration points will be reevaluated (normal calibration). If at least a two-point calibration has been performed and an offset correction of the electrode is wanted

(maintaining the existing slope values), perform a one-point calibration using the Offset option. To set the 1st Cal. Point:

• Use or to select the

• Press and use or to highlight the

1st Cal. Point

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

option.

Calibration

option.

DIRECT MEASUREMENT

To measure the TDS of a sample using the

• Press and then to select TDS measure mode.

• Select the

• Proceed as for the conductivity measurement (see

Measurement

DIRECT/AUTOHOLD MEASUREMENT

To measure TDS of a sample using the

• Select the section).

• Proceed as for the conductivity measurement. (see

ity Measurement

Direct

reading mode (see

section).

Direct / AutoHold

section)

reading mode (see

Direct

TDS Setup

section).

Direct / AutoHold

reading mode:

Conductivity

reading mode:

TDS Setup

Conductiv-

Edit Custom Buffers

If you want to use other buffers than the ones already memorized (standard buffers), the Edit Custom Buffers option is available, allowing you to set the desired pH buffers. Up to five pH custom buffers can be set.

RESISTIVITY MEASURERESISTIVITY MEASURE

RESISTIVITY MEASURE

RESISTIVITY MEASURERESISTIVITY MEASURE

Make sure the instrument has been calibrated before taking resistivity measurements.

DIRECT MEASUREMENT

To measure the resistivity of a sample using the mode:

• Press and then to select resistivity measure mode.

• Select the

• Proceed as for the conductivity measurement (see

Measurement

Direct

reading mode (see

section).

Resistivity Setup

Direct

reading

section).

Conductivity

MENTMENT

MENT

MENTMENT

To edit the Custom Buffers:

• Use

• Press and use or to highlight the

Edit Custom Buffers

• For a previous set value, press to set the custom buffer value to “----” if desired and confirm the setting by pressing , otherwise press to edit the selected custom buffer.

• While in edit custom buffer menu press to set the custom buffer value to 7.000 pH and then use or

• Press to exit custom buffer edit menu. If the Saving Confirmation is enabled, press to

or to select the

option.

to set the desired custom buffer value.

Calibration

option.

DIRECT/AUTOHOLD MEASUREMENT

To measure resistivity of a sample using the mode:

• Select the section).

• Proceed as for the conductivity measurement. (see

Measurement

Direct / AutoHold

section)

reading mode (see

Direct / AutoHold

Resistivity Setup

reading

Conductivity

accept the modified option, Otherwise, the modified option is saved automatically.

• Use

key to select the next custom buffer to be set or press to return to Calibration options.

to escape without saving or to return to the editing mode.

Edit Buffer Group

Accessing this option the user can edit the desired group of five pH buffers for automatic buffer recognition (Automatic Buffer Entry Type). If the Buffer Group already contains five pH buffers, at least one pH buffer has to be removed in order to add another buffer.

To set the Buffer Group:

• Use or to select the

• Press and use or to highlight the

Edit Buffer Group

• Press and use and to choose the pH buffer to be included in the buffer group.

• Press or to add / remove the selected pH buffer to / from the buffer group.

• Press

option.

to return to Calibration options and to save the changes.

Calibration

option.

Calibration Reminder

ytivitcudnoC

)mc/Sµ(

ytivitcudnoC

)mc/Sµ(

0.5 7.4 1.6 4.2

1.5 1.4 2.6 5.2

2.5 6.3 3.6 4.2

3.5 3.3 4.6 3.2

4.5 0.3 5.6 2.2

5.5 8.2 6.6 1.2

6.5 6.2 7.6 6.2

7.5 5.2 8.6 1.3

8.5 4.2 9.6 8.3

9.5 4.2 0.7 6.4

0.6 4.2

In order to have accurate readings, the instrument must be calibrated frecquently. Three options are available for the calibration reminder: Daily, Periodic or Disabled.

To set the Calibration Reminder:

• Use or to select the Calibration option.

• Press and use or to highlight the

Calibration Reminder

option.

• Press and use or to highlight the desired option.

• Press to confirm your selection or press

cancel operation.

Set Reminder Period

If choosing Daily or Periodic options for the Calibration Reminder, the Set Remind Period must be accessed in order to set the time interval until next calibration. The time interval between two calibrations can be set up to 1 day / 1 year for Daily / Periodic options.

Note: If Set Reminder Period parameter is accessed and the Calibration Reminder is disabled, a warning

message appears on the LCD informing the user that the reminder period can be set only if the Calibration Reminder is set as Daily or Periodic.

To set the Reminder Period:

• Use or to select the

Calibration

option.

• Press and use or to highlight the

Set Reminder Period

option.

• Press and use / to select next / previous entry to be edit.

• Press and use or to set the desired value, then press

• Press to return to the Calibration options. If the Saving Confirmation is enabled, press option, to escape without saving or to return to the editing mode. Otherwise, the modified

option is saved automatically.

to save the modified value.

to accept the modified

Stage 3 - this is an off-line test. To perform this test:

• Take the water sample from the previous test and increase its ionic strength for a pH measurement at 25 °C;

•

Record the pH and round it to the nearest 0.1 pH;

• Look up the corresponding conducivity value measured in Stage 2 above;

• If the conductivity is lower than the conductivity from the below table, then the sample has met the USP

requirements. Otherwise, the water didn’t meet the USP requirements.

To access the USP menu:

• Select the

Direct / USP

reading mode (see

Conductivity Setup

);

• Return to measure mode;

• Press (USP) and then select the desired USP stage.

DIRECT/USP MEASUREMENT

erutarepmeT

)C°(

ytivitcudnoC

)mc/Sµ(

erutarepmeT

)C°(

ytivitcudnoC

)mc/Sµ( 0 6.0 55 1.2 5 8.0 06 2.2

01 9.0 56 4.2 51 0.1 07 5.2 02 1.1 57 7.2 52 3.1 08 7.2 03 4.1 58 7.2 53 5.1 09 7.2 04 7.1 59 9.2 54 8.1 001 1.3 05 9.1

In this measure mode the user can check for ultra pure water using the United States Pharmacopeia standard (USP <645>).

This USP standard consists of three stages (one in-line and two off-line tests) as following: Stage 1 - this is an in-line test. To perform this test:

• Measure the temperature of the water and the uncompensated conductivity readings. The measurement may be performed in a suitable container or as in-line measurement.

• The temperature will be round down to the nearest 5 °C and look up the corresponding conductivity value in the below table.

• If the measured conductivity is lower than the conductivity in the table, then the water meets the USP requirements.

• Otherwise, proceed to Stage 2 testing.

Clear Calibration

Accessing this option, the existent pH calibration can be cleared. If the calibration is cleared, another calibration has to be performed.

To clear Calibration:

• Use or to select the Calibration option.

• Press and use or to highlight the

Clear Calibration

option.

• Press to clear calibration. A pop-up menu will be displayed asking for confirmation.

• Press to confirm or press to escape without saving and return to the Calibration options.

Sample ID

This option allows the user to give to the measured samples an identification number/name. Two Sample ID options are available:

ID Increment Mode

and

Edit Sample ID

ID Increment Mode

Two increment modes for the sample ID can be selected:

None – the sample ID will be fixed and it can be set alphanumerically. Automatic – the sample ID will be increased with 1 for each new log lot.

To set the ID Increment Mode:

• Use or to select the

Sample ID

option.

Stage 2 - this is an off-line test. To perform this test:

• Store the water sample in an enclosed clean container that has been rinsed previously with water of the same quality.

• Adjust the sample’s temperature to 25 °C and agitate the sample to ensure that it has equilibrated with ambient CO2.

• If the measured conductivity is less than 2.1 µS/cm, then the sample has met the USP requirements.

• Otherwise, proceed to Stage 3 testing.

• Press and use or to highlight the

ID Increment Mode

option.

• Press and use or to highlight the desired option.

• Press

to confirm your selection or press

cancel operation.

Edit Sample ID

This option allows the user to edit the sample ID. Note: If the ID Increment Mode is set to None, the sample ID can be set alphanumerically, otherwise it can be

set only numerically.

To edit the Sample ID:

• Use or to select the Sample ID option.

• Press and use or to highlight the

Edit Sample ID

• Press to confirm your selection.

• If the selected increment mode is None, the Text Editor menu will be displayed on the LCD, allowing you to enter the desired sample number/name by accepting the highlighted character which is added

in the text bar, using help the user to select the desired character. It is also possible to

delete the last character by positioning the cursor on the Backspace character and pressing .

• Press to return to Sample ID options. If the Saving Confirmation is enabled, press to accept the modified option, to escape without saving or to return

to the editing mode. Otherwise, the modified options are saved automatically.

• If the selected increment mode is Automatic, the desired sample ID value can be set using or .

• Press to save the current value or press cancel operation.

option.

. The and keys

CONDUCTIVITY MEASUREMENTCONDUCTIVITY MEASUREMENT

CONDUCTIVITY MEASUREMENT

CONDUCTIVITY MEASUREMENTCONDUCTIVITY MEASUREMENT

Make sure the instrument has been calibrated before taking conductivity measurements.

DIRECT MEASUREMENT

To measure the conductivity of a sample using the Direct reading mode:

• Press and then to select conductivity measure mode.

• Select the

• Submerge the conductivity probe and tap it repeatedly to remove any air bubbles that may be trapped inside the sleeve. Allow time for the reading to stabilize.

• The measured conductivity value will be displayed.

Note: If the reading is out of range, “-----” will be displayed.

DIRECT/AUTOHOLD MEASUREMENT

To measure conductivity of a sample using the Direct / AutoHold reading mode:

• Select the Direct / AutoHold reading mode (see

Setup

• If pressing , the “AutoHold” indicator will start blinking on the display until the stability criterion is reached. The

conductivity value will be frozen on the display, along with “AutoHold” indicator.

• To return to normal measure mode press .

Direct

reading mode (see

Conductivity Setup

Conductivity

Stability Criteria

This option allows the user to select the signal stability criterion for the measured parameter (pH, mV, ISE): Fast – this criterion will give faster results with less accuracy. Medium – this criterion will give medium speed results with medium accuracy. Accurate – this criterion will give slower results with high accuracy.

• Insert and rinse the probe in the first beaker in order to decontaminate it;

• Insert the probe in the second beaker;

• Tap the probe repeatedly to remove any air bubbles that may be trapped inside the sleeve.

• Enter in calibration mode by pressing

• Wait to stabilize;

• When the automatic standard recognition is selected, the calibration point will be automatically selected from the Hanna standard list (84 µS, 1413 µS, 5.0 mS,12.88 mS, 80.0 mS, 111.8 mS). The user can

also select the desired standard value by using and ;

• Otherwise (user standard), the pop-up will prompt for the custom standard value.

• Press to finish the calibration or to abort calibration.

;

To set the Stability Criteria:

• Use

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

or to select the

Stability Criteria

option.

Note: The calculated cell constant will be used for the whole range.

Multi Point Calibration

• Up to 4 calibration points can be performed in order to increase the measurement accuracy on the wide range;

• Select the multi point calibration (see

Calibration

• Repeat the steps from the single point calibration for each calibration point. The meter will calculate a cell constant corresponding to each calibration point;

• Press to exit calibration mode.

Note: For each range the corresponding cell constant will be displayed.

CELL CONSTANT CALIBRATION (edited by the user)

• A known value of the probe cell constant can be set by the user for the whole range (see

Setup

Note: When the user cell constant is used the old calibration (in solution) will be cleared.

);

Cell Constant

section)

Conductivity Setup

Conductivity

Reading Mode

This option allows the user to select between pH reading mode. If choosing the second option, the current reading can be frozen on the LCD when is pressed and if the stability criterion is reached. To set the Reading Mode:

• Use or to select the

• Press and use or to highlight the desired option.

• Press to confirm your selection or press to cancel operation.

Log

This option allows the user to edit the logging settings: and

New Lot.

Direct

and

Reading Mode

Direct / AutoHold

option.

Logging Type, Logging Data Configuration, Sampling Period

Logging Type

Three logging types are available: logged automatically at constant time intervals while with the Manual type the user has the capability to log a

measured value by pressing . With the AutoHold type the stored values are only the ones frozen on the LCD

Automatic, Manual

and

AutoHold

. With the Automatic type the values are

after the logging session was started. To set the Logging Type:

• Use

• Press and use or to highlight the

Logging Type

• Press and use or to highlight the desired option.

• Press to confirm your selection or press to cancel operation.

or to select the

option.

Log

option.

Logging Data Configuration

This option allows the user to select the parameters that accompany a logged value:

and

Sample ID, Instrument ID, Operator ID, Company Name, Aditional Info 1

To set the Logging Data Configuration:

• Use or to select the Log option.

• Press and use or to highlight the

Logging Data Configuration

• Press and use or to highlight the desired parameter to be logged in file.

• Press to confirm your selection and use or

to enable the parameter by selecting Yes or to disable

it by selecting No.

• Press to confirm your selection or press cancel operation.

option.

Aditional Info 2.

Date / Time, Calibration Data,

CONDUCTIVITY CALIBRATIONCONDUCTIVITY CALIBRATION

CONDUCTIVITY CALIBRATION

CONDUCTIVITY CALIBRATIONCONDUCTIVITY CALIBRATION

It is recommended to calibrate the instrument frequently, especially if high accuracy is required. The conductivity range should be recalibrated:

• Whenever the conductivity probe is replaced.

• At least once a week.

• Before USP measurements.

• After testing aggressive chemicals.

• When calibration reminder is activated (“Conductivity Cal Expired”).

• If the readings are far from the calibration point.

Note:TDS and Resistivity readings are automatically derived from the conductivity readings and no specific

calibration is needed.

OFFSET CALIBRATION

The meter allows the user to calibrate the probe for the offset.

• Set the meter for conductivity range;

• Select the automatic standard recognition (see

• Leave the dry probe in the air;

• Enter in calibration mode by pressing ;

• Wait to stabilize. The 0.000 µS calibration point will appear on the screen;

• Press to finish the probe offset calibration.

• Press to exit calibration mode or continue calibration in the other standard solutions. Note: The offset calibration can be performed only if it is perfomed first (no other calibration points present).

Clear the old calibration if it is present.

CELL CONSTANT CALIBRATION (in solution)

Conductivity Setup

Calibration

);

Single Point Calibration

• Select the single point calibration (see

• Pour a small quantity of the standard solution into a clean beaker. If possible, use plastic beakers to

minimize any EMC interferences.

• For accurate calibration and to minimize cross-contamination, use two beakers for each standard solution.

One for rinsing the probe and one for calibration.

Conductivity Setup

Calibration

);

Clear Calibration

Accessing this option, the existent salinity calibration ( To clear calibration:

• Use or to highlight the

• Press to clear calibration. A pop-up menu will be displayed to ask for confirmation.

• Press to confirm or press to cancel operation.

Salinity Scale

The meter uses three salinity scales: Natural Sea Water 1966, Practical Scale 1978, Percent Scale [%].

To select the salinity measurement scale:

• Press

• Press .

• Use or to select the

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

while in salinity measure mode.

Salinity Scale

Percent Scale

Clear Calibration

option.

) can be cleared.

option.

Sampling Period

This option allows the user to select the desired sampling period for automatic logging type.

To set the Sampling Period:

• Use or to select the

• Press and use or to highlight the

Sampling Period

• Press and use or to select the desired logging time interval.

• Press to confirm your selection or press to cancel operation.

option.

Log

option.

New Lot

Accessing this option, a new manual lot can be generated next time you want to manually log a measured value. Note: If New Lot option is accessed and the Logging Type is Automatic,

a warning message appears on the LCD informing the user that a new lot can be created only if the Logging Type is set as Manual.

To generate a New Lot:

• Use or to select the

Log

option.

Sample ID - see

Log - see

Alarm - see

three

Conductivity Setup

section.

• Press and use or to highlight the

New Lot

option.

• Press to generate a new manual lot. A pop-up menu will be displayed asking for confirmation.

• Press to confirm or press to escape without saving and return to the Log options.

Alarm

This option allows the user to edit the alarm settings: a continuous double beep will be heard, along with the “Alarm” indicator blinking on the LCD, each time the set limits in

Measure

mode are exceeded.

Alarm State

and

Alarm Limits

. If the

Alarm

option is enabled,

SALINITYSALINITY

SALINITY

SALINITYSALINITY

The Salinity Setup menu allows the user to set the parameters related to Salinity measurement and calibration.

SETUP SETUP

SETUP

SETUP SETUP

Alarm State

Three modes are available for the

Disabled

– the alarm will be disabled.

Inside Limts Outside Limits

To set the Alarm State:

• Use or to select the

• Press and use or to highlight the

• Press to confirm your selection or press

– the alarm will notify the user when the measured value is inside the set limits.

– the alarm will notify the user when the measured value is outside the set limits.

Alarm State

desired option.

cancel operation.

option.

Alarm State

option:

Alarm

option.

Alarm Limits

This option allows the user to set the alarm limits for the measured value. Note: The Alarm High value can not be lower than the Alarm Low value.

To set the Alarm Limits:

• Use or to select the

• Press and use or to highlight the

Alarm Limits

• Press and use / to select next / previous entry to be edit.

• Press

option.

and use or to set the desired

Alarm

option.

Accessing Salinity Setup

• Press select

• Press and then menu.

To access an

• Use or to highlight the desired option.

• Press to access the selected option.

The following is a description of the

Profile - see

Reading Mode - see

Temperature

This option allows the user to choose the temperature source and units. To access a Temperature options:

• Use or to highlight the Temperature option.

• Press to access Temperature option.

• Press to confirm your selection and use or to enable the parameter by selecting Yes or to disable it by selecting No.

while in

Salinity

range.

Salinity Setup

Conductivity Setup

Temperature Source, Temperature Unit

Measure

option:

Salinity Setup

section.

Resistivity Setup

mode and then to

to access

section.

- see

Salinity Setup

option screens.

Conductivity Setup

section.

value, then press

• Press to return to the Alarm options. If the Saving

to save the modified value.

TDS factor

Confirmation is enabled, press to accept the modified option, to escape without saving

With this option the user can set the TDS factor.

• Use or to highlight the TDS options.

• Press to confirm your selection and use or to increase / decrease the value.

• Press to confirm your selection or press to

cancel operation.

Sample ID - see

Log - see

Alarm - see

Conductivity Setup

section.

or to return to the editing mode. Otherwise, the modified option is saved automatically.

Isopotential Point

This option allows the user to edit the isopotential point of the electrode used for pH measurements. The isopotential point is the mV reading for an electrode at which temperature has no effect on the measurement. The ideal electrode has an isopotential point of 0.0 mV and 7.00 pH, while an actual electrode tipically deviates slightly from the ideal values.

If the actual isopotential pH for an electrode is known, it can be set by accessing this option. The isopotential pH limits (low and high) will be displayed on the LCD.

Note: If the isopotential point has been modified, recalibration must be performed for the pH channel. To set the Isopotential Point:

• Use or to select the

• Press

• Press to save the current value or press cancel operation.

pH Resolution

Accessing this option, the desired pH resolution can be set, with one (x.x), two (x.xx) or three (x.xxx) decimals. To set the pH Resolution:

• Use or to select the

and set the desired isopotential pH value using

or .

Isopotential Point

pH Resolution

option.

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

mVmV

SETUP SETUP

SETUP

mVmV

SETUP SETUP

The mV Setup menu allows the user to set the parameters associated with mV and Relative mV measurements.

TDSTDS

SETUP SETUP

TDS

SETUP

TDSTDS

SETUP SETUP

The TDS Setup menu allows the user to set the parameters related to the TDS measurement.

Accessing mV Setup

• Press

• Press and then to access mV Setup menu.

To access a mV Setup option:

• Use or to highlight the desired option.

• Press The following is a detailed description of the mV Setup option

screens.

Profile – See

Temperature – See

Sample ID – See

Stability Criteria – See

Reading Mode – See

Log – See

Alarm – See

Note: The Alarm Limits (Low and High) are set in mV units.

while in

to select mV / mV Rel range.

pH Setup

Measure

mode and then or

to access the selected option.

section.

pH Setup

section.

pH Setup

section.

Accessing TDS Setup

• Press while in select TDS range.

• Press and then

To access a TDS Setup option:

• Use or to highlight the desired option.

• Press to access the selected option.

The following is a description of the TDS Setup option screens.

Profile - see

Reading Mode - see

Temperature - see

Units

This option allows the user to set the TDS measuring range ppm(mg/L), ppt(g/L), AutoRanging

To select the suitable unit:

• Use or to highlight the

Conductivity Setup

Measure

mode and then to

section.

Resistivity Setup

Conductivity Setup

to access TDS Setup menu.

section.

TDS Units

• Press to confirm and then use or to highlight the convenient measuring range option.

• Press

cancel operation.

to confirm your selection or press to

Temperature - see

Units

The user can choose between Ohm, KOhm, MOhm, AutoRanging measuring modes.

To select the units:

• Use

• Press to confirm and then use or to highlight the desired unit.

• Press

Conductivity Setup

or to highlight the

to confirm or press to cancel operation.

section.

Units

option.

ISEISE

SETUP (HI 4522 SETUP (HI 4522

ISE

SETUP (HI 4522

ISEISE

SETUP (HI 4522 SETUP (HI 4522

The ISE Setup menu allows the user to set the parameters regarding ISE measurement and calibration. These parameters can be set specifically for each channel. The settings will be applied only to the focused channel.

Accessing ISE Setup

• Press select ISE range for the desired channel.

• Press and then

To access an

• Use

while in

ISE Setup

or to highlight the desired option.

option:

Measure

mode and then to

to access

ISE Setup

menu.

onlyonly

only

onlyonly

))

)

))

Sample ID - see

Log - see

Alarm - see

Conductivity Setup

section.

• Press

The following is a detailed description of the ISE Setup option screens.

Reading Mode

This option allows the user to select the desired reading mode:

Subtraction, Analyte Addition

are incremental (see ISE Theory section for details).

to access the selected option.

and

Analyte Subtraction

Direct, Direct / AutoHold, Known Addition, Known

. Except Direct and Direct / AutoHold, all the other methods

Direct

If using Direct reading mode, the ion concentration can be read directly from the instrument. Make sure the instrument was calibrated before taking measurements.

Direct / AutoHold

If using Direct/AutoHold reading mode, the ion concentration can be frozen on the LCD after starting the AutoHold mode.

Known Addition

In the Known Addition method a standard with a known concentration of the measured ion is added to the sample. The difference in mV potential is then used to calculate the concentration of the ion in the sample.

Known Subtraction

In the Known Subtraction method a standard with a known concentration is added to the sample, reacting with the ion to be measured. The stoichiometric ratio between standard and sample must be known. The ion concentration is then calculated using the difference in mV potential.

The method is especially usefull when there is no available ISE for the measured ion.

Analyte Addition

This method is similar to the Known Addition method, with the difference that an aliquot of sample is added to a standard of known concentration. The sample and standard contain the same ion to be measured. The ion concentration is then calculated using the difference in mV potential.

Analyte Subtraction

In the Analyte Subtraction method an aliquot of sample is added to a standard of known concentration, reacting with the ion to be measured. The stoichiometric ratio between standard and sample must be known. The ion concentration is then calculated using the difference in mV potential. The method is especially usefull when there is no available ISE for the measured ion.

To set the Reading Mode:

• Use or to select the

• Press and use or to highlight the desired option.

• Press cancel operation.

to confirm your selection or press

Reading Mode

option.

RESISTIVITYRESISTIVITY

RESISTIVITY

RESISTIVITYRESISTIVITY

The Resistivity Setup menu allows the user to set the parameters related with the resistivity measurements.

Accessing Resistivity Setup

• Press while in select resistivity range.

• Press and then to access menu.

To access a

• Use or to select the desired option.

• Press to confirm your selection.

The following is a description of the Resistivity Setup option screens.

Resistivity Setup

Measure

mode and then to

option:

SETUP SETUP

SETUP

SETUP SETUP

Resistivity Setup

Temperature – See Note: The Temperature Compensation option is also included in the Temperature parameter.

pH Setup

section.

Temperature Compensation

This option allows the user to enable / disable the temperature compensation for the measurement of Ion concentration.

To set the Temperature Compensation:

• Use or to select the

• Press and use or to highlight the

Temperature Compensation

• Press the Temperature Compensation.

and use or to enable / disable

option.

Temperature

option.

Profile - see

Reading Mode

This option allows the user to select between resistivity reading modes.

To set the

• Use or to select the

• Press and use or to highlight the desired option.

• Press cancel operation.

Conductivity Setup

Reading Mode:

to confirm your selection or press to

section.

Direct

and

Reading Mode

Direct/AutoHold

option.

Alarm

This option allows the user to define two alarm limits.

Alarm State

The following options are available:

Disabled

– the alarm will be disabled.

Inside Limits

value is inside the preset limits.

Outside Limits

value is outside the preset limits.

To set the alarm state:

• Use or to highlight the

• Press and use or to highlight the desired option.

• Press

– the alarm will notify the user when the measured

Alarm State

to confirm your selection or press

option.

to cancel operation.

Alarm Limits

This option allows the user to set the alarm limits for the measured value.

Note: The alarm high value cannot be lower than the alarm low

value.

To set the alarm limits:

• Hightlight the

Alarm Limits

option and then press

• Press

Note: If an ISE calibration was performed and the Temperature Compensation option is changed, a warning

message appears on the LCD informing the user to perform a new calibration or to set the previous option in order to perform accurate measurements.

Calibration

This option allows the user to set all the data regarding the Ion calibration process.

to confirm your selection or press

to cancel operation.

Standard Entry Type

Two entry modes for the standard solutions used for calibration are available: Manual Selection – when the calibration is started, the desired standard solution can be manually selected from

a standard solutions list (0.1 ppm, 1 ppm, 10 ppm, 100 ppm and 1000 ppm). Custom Standard – when the calibration is started, each standard solution can be manually set (in ppm units)

in a displayed pop-up menu. To set the Standard Entry Type:

• Use

• Press and use or to highlight the

Standard Entry Type

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

Calibration Reminder Set Reminder Period Clear Calibration

or to select the Calibration option.

option.

– See

pH Setup

section.

– See

pH Setup

section.

• Use or to select the low /high alarm limit and then press (Edit) .

• Use alarm value.

• Press

or to increase / decrease the selected

to return to the Alarm options.

Electrode Type

This option allows the user to select the desired Ion Selective Electrode used for measurements from a list: Ammonia, Bromide, Cadmium, Calcium, Carbon Dioxide, Chloride, Chlorine, Cupric, Cyanide, Fluoride, Iodide, Lead, Nitrate, Potassium, Silver, Sodium, Sulfate, Sulfide and five custom ISE. For the standard ISE it is possible to view the Ion constants (Name, Molar Weight and Electric Charge/Slope), while for the custom ISE all these constants can be manually set.

To set the Electrode Type:

• Use or to select the Electrode Type option.

• Press and use or to select the desired standard ISE or a custom one from the list. For standard ISE:

• Press to visualize the Ion constants and then press at any time to exit Ion Constants view mode.

• Press to confirm your selection and return to

options.

Setup

For custom ISE:

• Press to edit the Ion constants for the selected

ISE

Sampling Period

This option allows the user to select the desired sampling period for automatic logging.

To set the sampling period:

• Use or to highlight the

• Press and use or to select the desired option from

• Press to confirm your selection or press to cancel operation.

1, 2, 5, 10, 30

Sampling Period

seconds.

option.

custom ISE. Use desired Ion constant and press to enter Ion constant edit mode or to cancel operation.

• For the Ion name the Text Editor menu will be displayed on the LCD. Enter the desired information by accepting the highlighted character which is added in the text bar,

using . The and keys help the user to select the desired character. It is also possible

to delete the last character by positioning the cursor on the Backspace character and pressing . Press

to return to the Ion Constants menu. If the Saving Confirmation is enabled, press to accept the modified option, to escape without saving or to return to the editing mode. Otherwise, the

modified option is saved automatically.

• To set the appropriate Ion molar weight (in g/mol units) use or and then press to save

the current value or press

• To select the appropriate Electric Charge/Slope use or and then press . If the

Ion electric charge is None, its slope can be manually set

or to select the

to cancel operation.

New Lot

Accessing this option, the new manually logged readings will be put in a new log lot. To generate a new lot:

• Use or to highlight the

• Press to generate a new manual lot. A pop-up menu will be displayed to ask for confirmation.

• Press to confirm or press to escape without saving and return to the Log options.

Note: If

New Lot

option is accessed and the

informing the user that a new lot can be created only if the Logging Type is set on

New Lot

Logging Type

option.

Automatic

, a warning message appears on the LCD

Manual

Log

This option allows the user to edit the settings related to the logging feature, as following:

Logging Type

Three logging types are available:

Automatic logging

time intervals (see

Manual logging

AutoHold logging

auto-hold event occured. To set the sample logging type:

• Use

• Press and use or to choose from

Automatic, Manual

• Press to confirm your selection or press to cancel operation.

- the readings are logged automatically at constant

Sampling Period

( log on demand)- the readings are logged each time

is pressed.

- the readings are logged automatically at each

or to highlight the

Auto Hold

Automatic, Manual

option).

Logging Type

and

AutoHold.

option.

Logging Data Configuration

This option allows the user to select the parameters that accompany a logged value:

Operator ID, Company Name, Aditional Info 1

To set the logging data configuration:

• Use or to highlight the

Configuration

• Press and then use or to enable the parameter by selecting

each option.

• Press

Date/Time, Calibration Data, Sample ID, Instrument

and

Aditional Info 2

Logging Data

option.

Yes

or to disable it by selecting

to return to the previous menu.

for

by pressing on the LCD, in which the slope value can be set using

or . Press to save the

modified value or press Constants menu.

Note: If an ISE calibration was performed and a different Ion

Selective Electrode is selected (standard or custom), a warning message appears on the LCD informing the user to perform a new calibration or to select the previous ISE in order to perform accurate measurements.

Concentration Unit

Accessing this option, the user can select the desired concentration unit for the measured ion or chemical compound. The available concentration units are: ppt (g/L), ppm (mg/L), ppb (µg/L), mg/mL, M (mol/L), mmol/L and %w/v.

To set the Concentration Unit:

• Use option.

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

Sample ID – See

Stability Criteria – See

Log – See Note: The Logging Data Configuration option includes also the Ion Constants parameter. If you want it to appear

in the log reports, it must be enabled.

Alarm – See Note: The Alarm Limits (Low and High) are set in the selected concentration unit of the measured ion or chemical

compound and are displayed in a scientific format (value and value exponent).

or to select the Concentration Unit

pH Setup

section.

pH Setup

. A pop-up menu will be displayed

to return to the Ion

section.

pH Setup

section.

Isopotential Point

This option allows the user to edit the isopotential point of the electrode used for ion measurements. The ion selective electrodes have different isopotential points. If temperature compensation is desired for the ion measurements, the isopotential point value is absolutely necessary.

The isopotential point is always edited in ppm (mg/L) units. To set the Isopotential Point:

• Use or to select the Isopotential Point option.

• Press and use or to increase / decrease the isopotential point value.

• Press key to save the modified value.

Sample ID

This option allows the user to give to the measured samples an identification number/name. Two options are available:

ID Increment

and

Edit Sample ID

ID Increment

– the sample ID will be edited alphanumerically by the user.

None Automatic

every new log lot notification. To select the ID increment mode:

• Use

– the sample ID will be automatically incremented at

or to highlight the

ID Increment

option.

Sample ID

• Press Confirmation is enabled, press option,

the editing mode. Otherwise, the modified option is saved automatically.

Note: If an ISE calibration was performed and the Isopotential Point option is changed, a warning message

appears on the LCD informing the user to perform a new calibration or to set the previous option in order to perform accurate measurements.

ISE Significant Digits

Accessing this option, the number of ISE significant digits can be set, with one (x), two (xx) or three (xxx) significant digits.

To set the ISE Significant Digits:

• Use or to select the option.

• Press and use or to highlight the desired option.

• Press cancel operation.

to return to the Ion Setup options. If the Saving

to accept the modified

to escape without saving or to return to

ISE Significant Digits

to confirm your selection or press to

• Press the desired option.

• Press cancel operation.

and then use or to highlight

to confirm your selection or press

Edit Sample ID

This option allows the user to edit the sample ID (numeric - autoincrement mode, alphanumeric - user editable). To edit the Sample ID:

• Use or to select the Sample ID option.

• Press and use or to highlight the

• Edit numerically / alphanumerically the sample ID.

• Press to save the current sample ID or press

Edit Sample ID

to cancel operation.

option and then press .

Clear Calibration

Accessing this option, the existent conductivity calibration can be cleared. If the calibration is cleared, another calibration has to be performed.

To clear calibration:

• Use or to highlight the

• Press to clear calibration. A pop-up menu will be displayed asking for confirmation.

• Press to confirm or press to escape without saving and return to the Calibration options.

Probe Type

This option allows the user to obtain some information about the connected conductivity probe: name, default cell constant, range and rings number.

Units

The user can select the desired measurement unit. The available options are: µS, mS or

• Use or to highlight the

• Press to confirm your selection.

• Use or to select

• Press to confirm your selection or press to cancel operation.

µS, mS

Clear Calibration

Units

option.

AutoRanging.

option.

AutoRanging

pH CALIBRATIONpH CALIBRATION

pH CALIBRATION

pH CALIBRATIONpH CALIBRATION

Calibrate the instrument often, especially if high accuracy is required. The instrument should be recalibrated:

• Whenever the pH electrode is replaced.

• At least once a week.

• After testing aggressive chemicals.

• When “No pH Calibration” or “pH Calibration Expired” message appears on the LCD, in the Reminder messages area.

PREPARATION

Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences.

For accurate calibration and to minimize cross-contamination, use two beakers for each buffer solution. One for rinsing the electrode and one for calibration.

If you are measuring in the acidic range, use pH 7.01 or 6.86 as first buffer and pH 4.01/3.00 or 1.68 as second buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as first buffer and pH 10.01/

9.18 or 12.45 as second buffer. For extended range measurements (acidic and alkaline), perform a five-point calibration by selecting five of

the available buffers.

CALIBRATION PROCEDURE

Calibration has a choice of 8 memorized buffers: pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45 and up to 5 custom buffers.

For accurate measurements it is recommended to perform a five-point calibration. However, at least a two-point calibration is suggested.

pH Calibration screen description

Three buffer entry types are available: Automatic, Semiautomatic and Manual Selection. The default option is Manual Selection.

To calibrate the instrument using Manual Selection buffer entry type:

• Press . If the instrument was calibrated before and calibration was not cleared, the old calibration can be cleared by pressing . After 10 seconds, will no longer be available

from calibration screen. Note: It is very important to clear calibration history when a new electrode is used

because most errors and warning messages that appear during calibration depend on calibration history.

• Immerse the pH electrode and the temperature probe approximately 4 cm (1½”) into a buffer solution of your choice (pH 1.68, 3.00, 4.01, 6.86, 7.01,

9.18, 10.01, 12.45 or a custom buffer) and stir gently. The temperature probe should be close to the pH electrode.

• Select the used pH calibration buffer using wait...” message will appear on the LCD until the reading is stable or the buffer is validated.

• If the pH buffer is validated, will appear on the LCD. Press to update calibration. The calibration buffer will be added to the Calibrated Buffers list.

• Immerse the pH electrode and the temperature probe into the next buffer solution and follow the above procedure or press to exit calibration.

Notes: • The new added calibration point will replace an old one

if the difference between them is ±0.2 pH.

• If the existing stored calibration is full (five calibration points), a pop-up menu will be displayed on the LCD in

which you can select with or keys the buffer you want to replace with the current buffer. Press to delete the selected buffer and then

press to update calibration with the new buffer.

• When in MTC mode, if pressing after entering pH calibration and while a HANNA pH calibration buffer

with a x.xxx resolution is selected, a pop-up menu will be displayed on the LCD in which the temperature value can be adjusted using

or . The “Please

Calibration Reminder

This option allows the user to set the calibration reminder as

Periodic

Disabled

To set the calibration reminder:

• Use or to highlight the option.

minder

• Press to confirm your selection and then use

or to choose the desired option

• Press to confirm your selection or press to

cancel operation.

Daily

Calibration re-

Set Reminder Period

reminder - the user can set the time of day when the reminder is to appear.

Daily Periodic

reminder - the user can set the time from the last calibration (days, hours and minutes) after which the

reminder appears.

To set the reminder period:

• Use or to highlight the

option.

• Press and use / to select next /

previous entry to be edited.

• Press and use or to set the desired

value, then press to save the modified value.

• Press to return to the previous menu.

Set Reminder Period

. Press to save the new temperature value.

Calibration Points

The user can choose between To set the calibration points:

• Use or to highlight the option.

• Press to confirm your selection and then use or to choose the desired option

• Press to confirm your selection or press to cancel operation.

Single Point

and

Multi Points

Calibration Points

calibration.

Cell constant manual editing:

The conductivity probe can also be calibrated by entering the cell constant value.

To edit the cell constant value:

• Use or to highlight the

Cell Constant

option.

• When in MTC mode, if pressing calibration and while a custom pH calibration buffer is

selected, a pop-up menu will be displayed on the LCD in which the custom buffer and the temperature value can

be ajusted by pressing and then or

keys. Press to save the modified

value and then / to select next / previous value to be adjusted.

• When in ATC mode, if pressing after entering calibration and while a standard pH buffer (with a

x.xxx resolution) or a custom pH buffer is selected, a pop-up menu will be displayed on the LCD in which

the buffer value can be adjusted using buffer value.

• If the Automatic buffer entry type has been selected for the calibration procedure, the instrument will automatically select the closest buffer to the measured pH value from the edit buffer group (see

pH Setup

for details).

• If the Semiautomatic buffer entry type has been selected for the calibration procedure, the instrument will automatically select the closest buffers to the measured pH value from all the available buffers

and the used buffer value can be selected with or .

after entering

or . Press to save the new

• Press and then use or to increase / decrease the value.

• Press to preset the cell constant value to the probe auto-recognizable default cell constant.

• Press to confirm the new value or press to exit without modifying.

CALIBRATION MESSAGES

• Wrong buffer. Please check the buffer: this message appears when the difference between the pH

reading and the value of the selected calibration buffer is significant. If this message is displayed, check if you have selected the appropriate calibration buffer.

• Wrong buffer temperature: this message appears if the buffer temperature is out of the defined buffer

temperature range.

• Clean the electrode or check the buffer. Press <Accept> to update calibration: this message alerts the

user that some dirt or deposits could be on the electrode. Refer to the electrode Cleaning Procedure (see page 65).

• Slope too low. Please check the buffer / Slope too high. Please check the buffer: these messages appear

if the current slope is under 80% or over 110% of default slope. Recalibrate the instrument using fresh buffers.

• Slope too low. Press <Clear Cal> to clear old calibration / Slope too high. Press <Clear Cal> to clear old calibration: these messages appear as a result of an erroneous slope condition. Follow displayed instructions.

• Unrecognized buffer. Please check the buffer or the buffer list (for Semiautomatic and Automatic buffer entry

type): this message appears if the current buffer value is not close to any of the buffers from the buffer list/group. Check if the current buffer is present in the buffer list or the appropriate buffer group was selected.

• The current buffer was already calibrated. Press <Accept> to recalibrate in this point, or change the buffer: this message appears when calibration is performed in one of the previous calibrated buffers. Follow displayed instructions.

Compensation Coefficient

To set the compensation coefficient:

• Use or to highlight the

Coefficient

• Press using or to increase/decrease the value.

option.

(only for

Linear

temperature compensation)

Compensation

and set the desired compensation coefficient

• Press to save the current value or press cancel operation.

Calibration Cell Constant

The conductivity probe can be calibrated using the conductivity standards or by entering the cell constant of the probe by the user.

Using standard solutions:Using standard solutions:

Using standard solutions:

Using standard solutions:Using standard solutions:

The meter can be calibrated in a single or multi-points (up to four points), using 6 Hanna standards (84 µS, 1413

µS, 5.0 mS,12.88 mS, 80.0 mS, 111.8 mS) or using the custom standards.

The following options are available for calibration:

Standard Recognition

The user can choose between standards available) or used for calibration).

• Use or to highlight the

option.

tion

• Use or to choose from

option

Standard

• Press to confirm your selection or press to cancel operation.

Automatic

User Standard

recognition (from 6 Hanna

(when custom standards are

Standard Recogni-

Automatic

User

Temperature Unit

The user can choose from the units.

To set the temperature unit:

• Use or to highlight the option.

• Press and then use or to select

Celsius, Fahrenheit

• Press to confirm your selection or press to cancel operation.

Celsius, Fahrenheit

Kelvin

degrees unit

Kelvin

temperature

Temperature Unit

pH MEASUREMENTpH MEASUREMENT

pH MEASUREMENT

pH MEASUREMENTpH MEASUREMENT

Make sure the instrument has been calibrated before taking pH measurements.

DIRECT MEASUREMENT

To measure the pH of a sample using the Direct reading mode:

• Press

• Select the Direct reading mode (see pH Setup for details).

• Submerge the electrode tip and the temperature probe approximately 4 cm (1½”) into the sample to be tested. Allow time for the electrode to stabilize.

• The measured pH value will be displayed on the LCD, together with a short GLP information or “Default Calibration” message if no pH calibration was performed.

Note: If the reading is out of range, “-----” will be displayed on

the LCD.

and then to select

pH Measure

mode.

Reference Temperature

To set the reference temperature:

• Use or to highlight the option.

ture

• Press

/ decrease the value.

• Press to save or press

(only for

and then use

Linear

or to increase

Non-Linear

temperature compensation)

Reference Tempera-

to cancel operation.

DIRECT / AUTOHOLD MEASUREMENT

To measure pH of a sample using the Direct/AutoHold reading mode:

• Press

• Select the Direct/AutoHold reading mode (see details).

• Submerge the electrode tip and the temperature probe approximately 4 cm (1½”) into the sample to be tested. Allow time for the electrode to stabilize.

• The measured pH value will be displayed on the LCD. If pressing

LCD until the stability criterion is reached. The pH value will be frozen on the LCD, along with “AutoHold” indicator.

• To return to normal

Note: If the reading is out of range, “-----” will be displayed on

the LCD.

and then to select

, the “AutoHold” indicator will start blinking on the

Measure

mode press .

pH Measure

pH Setup

mode.

for

Outside Cal Range feature warns the user if the current reading is

0 1 2 3 4 5 6 7 8 9

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72 959.0 759.0 559.0 359.0 259.0 059.0 849.0 649.0 449.0 249.0

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53 228.0 028.0 918.0 718.0 618.0 418.0 318.0 118.0 018.0 808.0

out of the calibrated area. The calibrated area is that part of the pH range in which the calibration point assures an accurate reading. If the reading is taken out of the calibration area, the “Outside Cal Range” message will start blinking on the LCD. The calibrated area is calculated in according with the pH resolution used during the reading. To avoid having this message, the calibration points have to be well distributed in the desired measurement range.

If measurements are taken successively in different samples, it is recommended to rinse the electrode thoroughly with deionized water or tap water and then with some of the next sample to prevent cross-contamination and to condition the electrode before immersing it into the sample solution.

The pH reading is affected by temperature. In order to measure the pH accurately, the temperature effect must be compensated. To use the Automatic Temperature Compensation (ATC) feature, connect and submerge the temperature probe into the sample as close as possible to the electrode and wait for a few seconds.

If the temperature of the sample is known, Manual Temperature Compensation (MTC) can be performed by disconnecting the temperature probe.

Notes: • When in MTC mode, the temperature can be modified by pressing

for mV/

• When in ATC mode for pH, respectively TEMP for mV/Rel mV, “-----” will be displayed on the LCD if the measured temperature is under or over temperature range (–20.0 ºC to 120.0 ºC).

Rel mV Measure

from –20.0 ºC to 120.0 ºC. Press to save the new temperature value or press to return to

Measure

mode. The temperature value can be ajusted with or

mode.

for

pH Measure

Table for non-linear temperature compensation:

mode and

Temperature Compensation

The user can choose from the following options:

Linear -

the meter will compensate automatically the conductivity using the following formula:

mV & Relative mV MEASUREmV & Relative mV MEASURE

mV & Relative mV MEASURE

mV & Relative mV MEASUREmV & Relative mV MEASURE

mV/ORPmV/ORP

mV/ORP

mV/ORPmV/ORP

Oxidation-reduction potential (ORP) measurements provide the quantification of the oxidizing or reducing power of the tested sample.

To correctly perform a redox measurement, the surface of the ORP electrode must be clean and smooth.

MEASUREMENTS MEASUREMENTS

MEASUREMENTS

MEASUREMENTS MEASUREMENTS

MENTMENT

MENT

MENTMENT

where:

- conductivity at reference temperature

ref

- compensation coefficient

T - temperature

ref - reference temperature

Non-Linear -

Disabled -

To set the temperature compensation mode:

• Use or to highlight the

• Press and then use or to select

• Press to confirm your selection or press to cancel operation.

Whatever form of compensation is used, the reading will not be as accurate as taking a reading of the sample’s conductivity at the reference temperature.

recommended for measuring the conductivity of the natural water in accordance with the compensation

table on the next page.

the meter will display the conductivity with no temperature compensation.

Temperature Com-

pensation

Linear, Non-Linear

option.

Disabled

option.

DIRECT MEASUREMENT

To measure the mV of a sample using the Direct reading mode:

• Press and then to enter

• Select the Direct reading mode (see

• Submerge the tip of the ORP electrode (4 cm/1½”) into the sample to be tested and allow a few seconds for the reading to stabilize.

• The instrument will display the measured mV value on the LCD.

Note: If the reading is out of range, “-----” will be displayed

on the LCD.

DIRECT/AUTOHOLD MEASUREMENT

To measure mV of a sample using the Direct/AutoHold reading mode:

• Press

• Select the Direct/AutoHold reading mode (see details).

• Submerge the tip of the ORP electrode approximately 4 cm (1½”) into the sample to be tested and allow a few seconds for the reading to stabilize.

• The measured mV value will be displayed on the LCD. If pressing , the “AutoHold” indicator will start blinking on the LCD until the stability criterion is reached. The mV value

will be frozen on the LCD, along with “AutoHold” indicator.

• To return to normal

Note: If the reading is out of range, “-----” will be displayed

on the LCD.

and then to select

Measure

mode press .

mV Setup

mV Measure

for details).

mV Measure

mV Setup

mode.

for

Relative mVRelative mV

Relative mV

Relative mVRelative mV

To measure the Relative mV of a sample:

• Press

Measure

• The instrument will display the measured Relative mV value on the LCD, together with a short GLP information about the last calibration or “Not Calibrated” message if no Rel mV calibration was performed (no Rel mV offset set).

Notes: • If the measured mV potential is out of range, “-----”

• If pressing while in

• Before performing a Rel mV calibration, make sure

MEASUREMENTS MEASUREMENTS

MEASUREMENTS

MEASUREMENTS MEASUREMENTS

and then to enter

mode.

will be displayed on the LCD.

Relative mV

Relative mV Measure

mode, the Relative mV offset screen will be displayed on the LCD. Press

Relative mV or use or to adjust the Relative mV value and then press the current Relative mV. Press at any time

to return to

that the electrode is immersed into the sample to be measured.

Relative mV Measure

to clear the existing

mode.

to save

Temperature

From the source and units, as well as the temperature compensation mode, reference temperature and compensation coefficient.

To access a

• Use or to highlight the

• Press

Temperature

tion from the

menu the user can choose the temperature

option:

Temperature

Conductivity Setup

to access the

menu.

Temperature

option.

op-

Temperature Source

To set the temperature source:

• Use or to highlight the option.

• Press

Automatic

• Press cancel operation.

and then use or to select

Manual

temperature source.

to confirm your selection or press to

Temperature Source

Delete Profile

To delete one of the existing profiles:

• Use or to select the

• Press

Delete Profile

• Press the screen.

• Use or to select the desired profile and press

and use or to highlight the

option.

A list with all customised profiles will appear on

Profile

option.

• Press

Reading Mode

This option allows the user to select between

Direct/USP

To set the reading mode:

• Use or to select the

to return to the previous menu.

conductivity reading modes.

Direct, Direct / AutoHold,

Reading Mode

option.

ISE CALIBRATIONISE CALIBRATION

ISE CALIBRATION

ISE CALIBRATIONISE CALIBRATION

(HI 4522 only)(HI 4522 only)

(HI 4522 only)

(HI 4522 only)(HI 4522 only)

For greatest accuracy, it is recommended to calibrate the instrument frequently. The instrument should also be recalibrated whenever “No ISE Calibration” or “ISE Calibration Expired” message appears on the LCD, in the Reminder messages area.

Due to electrode conditioning time, the electrode must be kept immersed a few seconds to stabilize. The user will be guided step by step during calibration with easy to follow messages on the display. This will make the calibration a simple and error-free procedure.

PREPARATION

Pour small quantities of the standard solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences.

For accurate calibration and to minimize cross-contamination, use two beakers for each standard solution. One for rinsing the electrode and one for calibration.

Note: For accurate measurements, add the appropriate ISAB (Ionic Strength Adjustment Buffer) to the

calibration standards.

CALIBRATION PROCEDURE

Up to five-point calibration is possible, using five memorized standard solutions: 0.1, 1, 10, 100, 1000 ppm and 5 custom solutions.

The ISE calibration and measurement can be performed with or without temperature compensation. If the temperature compensation option is enabled, the isopotential point of the electrode must be set in ISE Setup in order to perform correct concentration measurements.

Before calibrating, make sure that the appropriate Electrode Type has been selected in ISE Setup according to the measured Ion/compound.

ISE Calibration screen description

• Press and use or to highlight the desired option.

• Press to confirm your selection or press cancel operation.

Two standard entry types are available: ManualSelection and Custom Standard. The default option is Manual Selection.

A. To calibrate the instrument using

• Press . If the instrument was calibrated before and calibration was not cleared, the old calibration can be cleared by pressing . After 10 seconds, will no longer be available.

Note: It is very important to clear calibration history when a new electrode is used

because most errors and warning messages that appear during calibration depend on calibration history.

• Add ISAB to all standard solutions.

• Immerse the Ion Selective Electrode and the temperature probe approximately 4 cm (1½”) into the less concentrated standard solution and stir gently.

• Select the used standard solution concentration with or . The “Please wait...” message will appear on the LCD until the reading is stable or the concentration is validated.

Note: When in ATC mode, if pressing

concentration value can be adjusted using or . Press to save the new concentration value.

• If the standard solution concentration is validated, will appear on the LCD. Press to update calibration. The calibration point value will be added to the Calibrated Standards list.

• The “Please wait...” message will appear on the LCD for 10 seconds and until the reading is stable, time in which immerse the ion selective electrode and the temperature probe into the next standard solution and

follow the above procedure or press to exit calibration.

B. To calibrate the instrument using

• Press . If the instrument was calibrated before and calibration was not cleared, the old calibration can be cleared by pressing . After 10 seconds, will no longer be available.

Note: It is very important to clear calibration history when a new electrode is

used because most errors and warning messages that appear during calibration depend on calibration history.

• Add ISAB to all standard solutions.

• Immerse the Ion Selective Electrode and the temperature probe approximately 4 cm (1½”) into the less concentrated standard solution and stir gently.

• When the stability condition is reached and 10 seconds elapsed, a pop-up menu will appear on the LCD in which the standard concentration value can be set.

• Press

/ to select next/previous entry to be edit. Press and use or

Manual Selection

a pop-up menu will be displayed on the LCD in which the

Custom Standard

standard entry type:

entry type:

Save Current Profile

To save the current profile:

• Use or to select the

• Press and then select

Text Editor

box will be displayed on the LCD.

• Enter the desired profile name by using and to highlight the desired character and then press to add it to

the text bar. It is also possible to delete the last character by positioning the cursor on the Backspace character ( ) and pressing .

• Press to return to the Profile options.

Note: The saved profile will automatically become the current profile.

Profile

option.

Save Current Profile

option. The

Load Profile

To load one profile:

• Use or to select the

• Press

Load Profile

• Press on the screen.

• Use or to select the desired profile and press

and use or to highlight the

option.

A list with all customised profiles will be displayed

confirm or

Profile

option.

to exit without selecting.

to set the desired value. Press to save the modified value and then press

to store the set concentration value.

ConductivityConductivity

Conductivity

ConductivityConductivity

The Conductivity Setup menu allows the user to set the parameters related to the conductivity measurement.

Accessing Conductivity Setup

• Press while in select the

• Press and then to access

Setup

To access a conductivity setup options:

• Use or to highlight the desired option.

• Press exit setup.

The following is a detailed description of the option screens.

Profile

Choosing this option the measuring and the calibration mode can be customized. Up to 10 profiles can be defined by the user.

The available options are:

Conductivity

menu.

Measure

mode and then to

measure mode.

to access the selected option or to

Conductivity Setup

SETUP SETUP

SETUP

SETUP SETUP

Conductivity

• If the standard solution concentration is validated, calibration. The calibration point value will be added to the Calibrated Standards section.

• The “Please wait...” message will appear on the LCD until the reading is stable and 10 seconds elapsed, time in which immerse the Ion Selective Electrode and the temperature probe into the next standard solution

and follow the above procedure or press to exit calibration.

Notes: • The new added calibration point will replace an old

one if the difference between them is less than 20% of the standard solution.

• If the existing stored calibration is full (five calibration points), a pop-up menu will be displayed on the

LCD in which you can select with the standard solution you want to replace with the current one. Press

calibrated point and then press calibration with the new standard solution.

• If the isopotential point of the electrode is unknown, the ion calibration and measurements can be performed only without temperature compensation (see ISE Setup, Temperature option for details, page 33). When the temperature compensation option is disabled, at least a two-point calibration must be performed in order to perform ISE measurements. Otherwise, “-----” will appear on the LCD.

to delete the selected

will appear on the LCD. Press to update

to update

Save Current Profile:

Load Profile:

Delete Profile:

load from available profiles.

delete a profile.

save the current profile.

• When in MTC mode, if pressing after entering ISE calibration and while a standard solution is

selected, a pop-up menu will be displayed on the LCD in which the concentration and the temperature value

can be ajusted by pressing and then or keys. Press to save the modified value and then / to select

next /previous value to be adjusted.

CALIBRATION MESSAGES

• Wrong standard solution. Please check the standard solution: this message appears when the

difference between the ppm reading and the value of the selected standard solution concentration (ppm)

is significant. If this message is displayed, check if you have selected the appropriate calibration standard.to be tested. Allow time for the electrode to stabilize.

• Wrong new slope. Please check the standard solution: this message appears if the current slope

exceeds slope window (50% to 120% of default slope for the corresponding ion charge - see ISE Theorysection for details). Recalibrate the instrument using fresh standards.

• Wrong old slope. Press <Clear Cal> to clear old calibration: this message appears as a result of

an erroneous slope condition. Press

to clear old calibration and restart calibration.

• Difference between standards temperature is too high. Press <Accept> to update the calibration or clear old calibration: this message appears when the difference between two standards temperature is greater than 5 ºC. Recalibrate the instrument while paying attention to the standards temperature.

where: C

- the sample concentration;

SAMPLE

- the standard concentration;

STD

V V

and VT = V

- the sample volume;

SAMPLE

- the standard volume;

STD

SAMPLE

+ V

STD

∆E - the difference of potential from the electrode;

S - the electrode slope, determined in a

previous calibration;

f - the stoichiometric ratio between sample

and standard;

where: C

C V V

and VT = V

- the sample concentration;

SAMPLE

- the standard concentration;

STD

- the sample volume;

SAMPLE

- the standard volume;

STD

+ V

SAMPLE

STD

∆E - the difference of potential from the electrode;

S - the electrode slope, determined in a

previous calibration;

f - the stoichiometric ratio between sample

and standard;

Example 1

You have sulfide samples and you are adding One mole sulfide sample reacts with 2 moles silver standard (

. The reaction is:

= ½).

Example 2

You have sulfide samples and you are adding One mole sulfide sample reacts with 1 mole lead standard (

. The reaction is:

= 1).

Analyte Addition and Analyte Subtraction

Analyte Addition and Subtraction are variations of the previous two methods.

ISE MEASUREMENTISE MEASUREMENT

ISE MEASUREMENT

ISE MEASUREMENTISE MEASUREMENT

(HI 4522 only)(HI 4522 only)

(HI 4522 only)

(HI 4522 only)(HI 4522 only)

Make sure the instrument has been calibrated before taking ISE measurements. When using one of the incremental methods for measurement and no or only a one-point ISE calibration was

performed, a warning message will be displayed on the LCD, notifying the user that at least a two-point ISE calibration must be performed.

For accurate measurements, add the appropriate ISAB (Ionic Strength Adjustment Buffer) to both samples and standards, if necessary. If ISAB is added to the calibration standards, it is important to add ISAB also to the samples to be measured.

DIRECT MEASUREMENT

To measure the concentration of a sample using the Direct reading mode:

• Press

and then to select

• Select the Direct reading mode (see

ISE Setup

ISE Measure

for details).

mode.

• Add ISAB to the sample solution.

• Submerge the Ion Selective Electrode tip and the temperature probe approximately 4 cm (1½”) into the sample to be tested. Allow time for the electrode to stabilize.

• The measured concentration value will be displayed on the LCD in the selected units.

Note: If the reading is out of range, “-----” will be dis-

played on the LCD.

With Analyte Addition,

sample (analyte) is added

to an ion standard being measured. The standard and sample contain the same ion. mV are taken before and after the sample addition. From the mV the analyte concentration is determined.

With

Analyte Subtraction,

sample (analyte) is added to an ion standard

being measured. The analyte reacts with the measured ion in a known manner thus removing measured ions from the solution. From the change in mV the concentration of the analyte is determined.

DIRECT/AUTOHOLD MEASUREMENT

To measure the concentration of a sample using the Direct / AutoHold reading mode:

• Press and then to select

ISE Measure

mode

for the selected channel.

• Select the Direct/AutoHold reading mode (see

ISE Setup

for

details).

• Add ISAB to the sample solution.

• Submerge the Ion Selective Electrode tip and the temperature probe approximately 4 cm (1½”) into the sample to be tested. Allow time for the electrode to stabilize.

• The measured concentration value will be displayed on the LCD. If pressing will start blinking on the LCD until the stability criterion is reached. The concentration value will be frozen on the

LCD, along with “AutoHold” indicator.

• To return to normal

Note: If the reading is out of range, “-----” will be displayed on the LCD. Note: Press at any time to stop the measurement and return to

KNOWN ADDITION

To measure the concentration of a sample using the Known Addition incremental method:

• Press and then to select mode for the selected channel.

• Select the Known Addition method (see

• Press to start the measurement. The first step of the method will be displayed on the LCD in which the user is notified

to add the appropriate ISA Buffer Volume (if different from 0 mL) to the sample and to immerse the electrode into the Sample Volume. The method parameters are also displayed on the LCD.

• Press to set the method parameters. Press / to select next/previous parameter to be edit, then press and use or to set the desired parameter value. Press to save the modified value and then press to exit method parameters edit menu.

• Make sure that the set parameters values are the used ones and submerge the Ion Selective Electrode tip approximately 4 cm (1½”) into the sample to be tested.

• Press to take the first mV reading.

Measure

mode press .

ISE Setup

ISE Measure

for details).

, the “AutoHold” indicator

ISE Measure

mode.

Actual samples that are more concentrated have much smaller activity coefficients ( inert background salt to standards and samples stabilizes the activity coefficient so that concentration measurements may be made directly. Hanna’s Ionic Strength Adjustment Buffer (ISAB) formulations also may optimize pH, and complex interferences in addition to standardizing the ionic strength.

The Nernst equation can be rewritten:

ION SELECTIVE ANALYSIS METHODS

Direct AnalysisDirect Analysis

Direct Analysis

Direct AnalysisDirect Analysis

This method is a simple procedure for measuring multiple samples. It should only be used in the linear working regions of the sensor. A direct reading instrument such as the HI 4522 determines concentration of the unknown by a direct reading after calibrating the instrument with the standards. The instrument is calibrated as described in “ISE CALIBRATION” section, with two or more freshly made standards that are in the measurement range of the unknowns. Ionic strength adjustment is made to samples and standards. Unknowns are measured directly by the instrument. At lower concentrations, in non-linear regions of the electrode response, multiple calibration points will extend measurements to a practical detection limit. Calibrations must be performed more frequently in these cases.

Incremental MethodsIncremental Methods

Incremental Methods

Incremental MethodsIncremental Methods

Incremental methods are useful for the measurement of samples whose constituents are variable or concentrated. Incremental techniques can reduce errors from such variables as temperature, viscosity, or pH extremes and will provide indirect analysis of ions for which there is no ISE sensor for a direct measurement. There are four different incremental methods for sample measurement. They are Known Addition, Known Subtraction, Analyte Addition and Analyte Subtraction. HI 4522 allows the analyst to use these techniques as a simple routine procedure thus eliminating calculations or tables. The method once set up can be used for repetitive measurements on multiple samples.

Known Addition and Known Subtraction

Known addition,

With same ion. mV are taken before and after the standard addition. From the mV the sample concentration is determined.

standard is added

to a sample being measured. The standard and sample contain the

<1). The addition of an

• When the reading is stable, press mV reading. The second step of the method will be displayed

on the LCD in which the user is notified to add the Standard Volume of set concentration to the sample. The method parameters are also displayed on the LCD.

to store the first

With

Known subtraction, with the measured ion in the sample in a known manner thus removing measured ions from the solution. From the change in mV the concentration of the sample is determined.

a known standard is added to an ionic sample

being measured. The standard reacts

ISE THEORYISE THEORY

SEICEPS

EPOLS

)edaced/Vm(

noitactnelavonoM 61.95+

noinatnelavonoM 61.95–

noitactnelaviD 85.92+

noinatnelaviD 85.92–

ISE THEORY

ISE THEORYISE THEORY

An Ion Selective Electrode (ISE) is an electrochemical sensor that changes voltage with the activity or concentration of ions in solutions. The change in voltage is a logarithmic relationship with concentration and is expressed by the Nernst equation:

• Press

to change the desired method parameters as indicated in the first step of the method.

• Press to take the second mV reading.

• When the reading is stable, press to store the second mV reading. The ISE measurement

results will be displayed on the LCD.

• Press to log the current results into a ISE Method Report or press to return to

ISE Measure

mode.

where: E - the measured voltage;

- standard voltage and other standard system voltages;

E a - the activity of the ion being measured; S - the Nernst slope factor and is derived from thermodynamic principles:

R - the universal gas constant (8.314 J*K-1*mol-1); T - the temperature in degrees Kelvin; F - the Faraday’s constant (96,485 C*mol-1); n - the ion charge.

The slope may be positive or negative depending upon the ion charge (n).

Activity and concentration are related by an “activity coefficient”, expressed as:

where: a - the activity of the ion being measured;

- the activity coefficient;

C - the concentration of the ion being measured.

In very dilute solutions

approaches 1 so activity and concentration are the same.

• If pressing , the method parameters can be changed and the sample concentration will be recalculated.

• Press to start another measurement.

Note: Press at any time to stop the measurement and return to

ISE Measure

mode.

KNOWN SUBTRACTION

To measure the concentration of a sample using the Known Subtraction incremental method:

• Press

and then to select

• Select the Known Subtraction method (see

ISE Measure

ISE Setup

mode for the selected channel.

for details).

• Press to start the measurement. The first step of the method will be displayed on the LCD in which the

user is notified to add the appropriate ISA Buffer Volume (if different from 0 mL) to the sample and to immerse the electrode into the Sample Volume. The method parameters are also displayed on the LCD.

• Press to set the method parameters. Press / to select next / previous

parameter to be edit, then press and use or to set the desired parameter value. Press to save the modified value and then press to exit method parameters edit

menu.

Note: For the Known Subtraction method, the stoichiometric ratio (factor) between standard and sample

must also be set in order to take accurate measurements.

• Make sure that the set parameters values are the used ones and submerge the Ion Selective Electrode tip

approximately 4 cm (1½”) into the sample to be tested.

• Press to take the first mV reading.

• When the reading is stable, press to store the first mV reading. The second step of the method

will be displayed on the LCD in which the user is notified to add the Standard Volume of set concentration to the sample. The method parameters are also displayed on the LCD.

• Press

to change the desired method parameters as indicated in the first step of the method.

• Press

• When the reading is stable, press to store the second mV reading. The ISE measurement

to take the second mV reading.

results will be displayed on the LCD.

• Press Report or press to return to

• If pressing , the method parameters can be changed and the sample concentration will be recalculated.

• Press to start another measurement.

Note: Press at any time to stop the measurement and

return to

ANALYTE ADDITION

To measure the concentration of a sample using the Analyte Addition incremental method:

• Press

• Select the Analyte Addition method (see

• Press to start the measurement. The first step of the method will be displayed on the LCD in which the user is notified to add the appropriate ISA Buffer Volume (if different from 0 mL) to the standard

and to immerse the electrode into the Standard Volume of set concentration. The method parameters are also displayed on the LCD.

• Press to set the method parameters. Press / to select next / previous parameter to be edit, then press and use or to set the desired parameter value. Press

• Make sure that the set parameters values are the used ones and submerge the Ion Selective Electrode tip approximately 4 cm (1½”) into the standard solution.

• Press to take the first mV reading.

• When the reading is stable, press to store the first mV reading. The second step of the method will be displayed on the LCD in which the user is notified to add the Sample Volume to the standard

solution. The method parameters are also displayed on the LCD.

• Press to change the desired method parameters as indicated in the first step of the method.

• Press

• When the reading is stable, press results will be displayed on the LCD.

• Press to log the current results into a ISE Method Report or press to return to

to log the current results into a ISE Method

ISE Measure

and then to select

mode.

ISE Setup

to save the modified value and then press to exit method parameters edit menu.

to take the second mV reading.

mode.

ISE Measure

to store the second mV reading. The ISE measurement

mode.

for details).

ISE Measure

mode.

• If pressing

• Press to start another measurement.

Note: Press at any time to stop the measurement and return to

ANALYTE SUBTRACTION

To measure the concentration of a sample using the Analyte Subtraction incremental method:

• Press and then to select

• Select the Analyte Subtraction method (see

and to immerse the electrode into the Standard Volume of set concentration. The method parameters are also displayed on the LCD.

• Press to be edit, then press and use or to set the desired parameter value. Press

to save the modified value and then press to exit method parameters edit menu.

Note: For the Analyte Subtraction method, the stoichiometric ratio (factor) between standard and sample

must also be set in order to take accurate measurements.

• Make sure that the set parameters values are the used ones and submerge the Ion Selective Electrode tip approximately 4 cm (1½”) into the standard solution.

• Press to take the first mV reading.

• When the reading is stable, press to store the first mV reading. The second step of the method will be displayed on the LCD in which the user is notified to add the Sample Volume to the standard

solution. The method parameters are also displayed on the LCD.

• Press to change the desired method parameters as indicated in the first step of the method.

• Press to take the second mV reading.

• When the reading is stable, press to store the second mV reading. The ISE measurement results will be displayed on the LCD.

• Press

• If pressing , the method parameters can be changed and the sample concentration will be recalculated.

• Press

Note: Press at any time to stop the measurement and return to

, the method parameters can be changed and the sample concentration will be recalculated.

ISE Measure

ISE Setup

to set the method parameters. Press / to select next/previous parameter

to log the current results into a ISE Method Report or press to return to

to start another measurement.

mode.

for details).

ISE Measure

mode.

ISE Measure

mode.

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Hanna Instruments HI 4522 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual