Hanna Instruments HI 4113 Instruction Manual
HI 4113
NITRATE
COMBINATION
HI
4000-50
SENSOR HANDLE
Instruction Manual
HI 4013HI 4013
HI 4013
HI 4013HI 4013
HI 4113HI 4113
HI 4113
HI 4113HI 4113
Nitrate Ion
Selective Electrode
Half-cell
Combination
1
HI 4013 Nitrate Half-cellHI 4013 Nitrate Half-cell
HI 4013 Nitrate Half-cell
HI 4013 Nitrate Half-cellHI 4013 Nitrate Half-cell
HI 4113 Nitrate Combination ElectrodeHI 4113 Nitrate Combination Electrode
HI 4113 Nitrate Combination Electrode
HI 4113 Nitrate Combination ElectrodeHI 4113 Nitrate Combination Electrode
I. I.
Introduction:Introduction:
I.
Introduction:
I. I.
Introduction:Introduction:
The Hanna HI 4013 and HI 4113 are ion selective electrodes designed for the measurement of nitrate ions in
aqueous solutions. They utilize a replaceable sensing module that contains an organic polymer membrane that is
sensitive to nitrate ions. The HI 4013 is a half-cell electrode
that requires a separate reference. The HI 4113 is a combination ion selective electrode.
IIII
SpecificationsSpecifications
II.
Specifications
IIII
SpecificationsSpecifications
Type: PVC membrane with or-
ganic ion exchanger
Ion measured: Nitrate (NO
Measurement range: 0.1 M to 1X 10
-
)
3
-5
M
6200 to 0.62 ppm
Interference:
Organic solvents and cationic detergents must be absent.
Ratio of interfering ion to NO
indicated below:
300 for F
-
must be less than the ratio
3
-
fluoride
100 for Cl-chloride
-
2-
3
-
2
-
4
carbonate
nitrite
iodide
perchlorate
4 for CO
2 for NO
0.01 for I
0.0045 for ClO
Operating Temperature: 0-40°C
Operating pH: 3 to 8 pH (see Section
XIII)
Dimensions: 12 mm (OD) X 120 mm
nominal insertion
(0.47” X 4.72”)
Connection: BNC
2
III. III.
Theory of OperationTheory of Operation
III.
Theory of Operation
III. III.
Theory of OperationTheory of Operation
::
:
::
The HI 4013 and HI 4113 nitrate electrodes are potentiometric devices used for the rapid determination of free nitrate ions in water, emulsified foods and plant samples.
The electrode functions as a sensor or ionic conductor. The
HI 4013 requires a separate reference electrode to complete
its electrolytic circuit. The HI 4113 is a combination electrode with a Ag/AgCl reference electrode with gel stabilized
Cl- electrolyte in it’s inner chamber. The external reference
chamber is refillable. The PVC membrane used on the
sensor is impregnated with the organic ion exchanger. This
organic ion exchanger is considered a carrier ionophore in
that it is capable of shielding and carrying the charged
nitrate ion in it’s polar cage freely through the apolar
regions of the membrane. A charge imbalance developes
between the test solution and internal cell of the sensor.
This voltage changes in response to the sample’s ion activ-
ity. When the ionic strength of the sample is fixed, the
voltage is proportional to the concentration of nitrate ions in
solution. The sensor follows the Nernst Equation:
E = E
+ 2.3 RT/nF log A
a
ion
E = observed potential
Ea = Reference and fixed internal voltages
R = gas constant (8.314 J/K Mol)
n = Charge on ion (-1)
A
= ion activity in sample
ion
T = absolute temperature in K
F = Faraday constant (9.648 x 104 C/equivalent)
3
IV. IV.
Design elements of the HI 4013 and HI 4113Design elements of the HI 4013 and HI 4113
IV.
Design elements of the HI 4013 and HI 4113
IV. IV.
Design elements of the HI 4013 and HI 4113Design elements of the HI 4013 and HI 4113
electrodeselectrodes
electrodes
electrodeselectrodes
Cap
HI
4000-50
HANDLE
R
SENSO
Sensor
Handle
O-Ring
Sensing
module
Upper Cap
Upper
Threads
O-Ring
Ceramic Junction
on Inner Stem
Liquid junction
Spring
Fill Hole
O-Ring and
Plug
Outer Sleeve
Sensing Module
4
V. V.
Equipment required:Equipment required:
V.
Equipment required:
V. V.
Equipment required:Equipment required:
• The HI 4013 requires the Hanna HI 5315 Double
junction reference electrode with HI 7078 as external
electrolyte.
• Hanna HI 4222 pH/ISE/mV meter or other suitable
ion or pH/mV meter (Note: log/linear graph paper is
useful if an ISE (ion) meter is not available).
• Hanna HI 180 magnetic stirrer or equivalent with
magnetic stirring bars (Note: Isolate beakers from stirrer motor heat by placing insulating material such as
foam or cork between them).
• Hanna HI 76404 electrode holder or equivalent.
• Plastic beakers (HI 740036P) or other suitable mea-
surement vessel.
VI. VI.
Solutions RequiredSolutions Required
VI.
Solutions Required
VI. VI.
Solutions RequiredSolutions Required
Standards for Nitrate Measurements
0.1 M sodium nitrate standard, 500 mL HI 4013-01
100 ppm nitrate standard, 500 mL HI 4013-02
1000 ppm nitrate standard, 500 mL HI 4013-03
Ionic Strength Adjuster
ISA, 500 mL HI 4013-00
Using volumetric pipettes and glassware make dilutions to
bracket the concentration of the samples. Store samples in
plastic bottles. Standards with concentrations < 10
-3
should be prepared daily.
Two mL of Hanna ISA HI 4013-00 should be added to 100
mL sample or standard.
ISISA is Interferent suppressent ISA. Dissolve 17.32g
Al2(S04)3.H20, 3.43.g Ag2SO4, 1.28g H3BO4, 2.52g
H2NSO3H in approximately 800 mL deionized water. Adjust
pH to 3 with 0.1 N NaOH. Dilute to 1 liter. Store in a dark
colored container.
50 mL of ISISA should be added to each 50 mL sample or
standard.
M
VIIVII
General GuidelinesGeneral Guidelines
VII.
General Guidelines
VIIVII
General GuidelinesGeneral Guidelines
• Ensure the o-ring is installed on modules before screw-
ing into the sensor handle or inner stem.
5
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