Hanna Instruments HI 3897 User Manual

Instruction Manual

HI 3897

Extra VirginExtra Virgin

Extra Virgin

Extra VirginExtra Virgin

Olive Oil Acidity Olive Oil Acidity

Olive Oil Acidity

Olive Oil Acidity Olive Oil Acidity

est Kitest Kit

est Kit

est Kitest Kit

www.hannainst.com

Dear Customer, Thank you for choosing a Hanna Product. Please read the

instructions carefully before using the chemical test kit. It will provide you with the necessary information for correct use of the kit. Remove the chemical test kit from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any noticeable damage, notify your Dealer or the nearest Hanna office immediately. Each kit is supplied with:

• 1 HI 180IMD magnetic stirrer;

• HI 3897-0 Titrant Solution, 1 bottle (20 mL);

• Organic Solvent, 6 bottles (40 mL each) with one magnetic bar each;

• 1 calibrated syringe of 5.0 ml;

• 1 calibrated syringe of 1.0 ml with tip.

Note:Note:

Note:Any damaged or defective item must be returned in

Note:Note:

Specifications

Range 0 to 1% acidity Smallest Increment 0.01 ml = 0.01 % Analysis Method Titration Sample Size 4.6 mL (or 4.0 g) Number of Tests 6 Case Dimensions 112 x 390 x 318 mm Shipping Weight 3000 g

SPECIFICATIONSSPECIFICATIONS

SPECIFICATIONS

SPECIFICATIONSSPECIFICATIONS

its original packing materials.

Significance and Use

Acidity defined as oleic acid, is a parameter that indicates the oil freshness: a high acidity value shows the oil is becoming rancid, thus the oil quality is decreasing. According

CEE 2568/91CEE 2568/91

CEE 2568/91 regulation, olive oil is called extra virgin

CEE 2568/91CEE 2568/91 when its acidity is below 1%: see table 1. Acidity expressed in oleic acid is used to discriminate extra virgin olive oil from other olive oils. It indicates an extraction process made soon after the olive harvesting and with natural, non chemical methods.

Table 1

Chemical Reaction

The oil sample is firstly dissolved in an organic solvent reagent (ethanol/ether); then it is titrated with a basic solution (OH-). The final point of the titration is indicated by the change in color of the solution, which turns from yellow/green of the oil to pink.

Instructions

READ ALL INSTRUCTIONS BEFORE USING THE TEST KIT

CAUTION! Carefully open the bottle of the Organic Solvent

reagent because it may be under pressure!

ISTR3897R2 07/04

• Remove the cap from 1 bottle of Organic Solvent reagent. Place the bottle on the magnetic stirrer and start a gentle stirring.

• Using the 1.0 ml syringe with tip, add the HI 3897-0 Titrant Solution reagent dropwise. Continue adding the Titratant Solution reagent until the solution in the bottle changes from colorless to a persistent Replace the cap.

NoteNote

Note: stop adding the HI 3897-0 Titrant Solution

NoteNote reagent to the bottle light pink colored.

• Take 4.6 ml of oil sample by use of the 5.0 ml syringe. Alternatively, weight about 4.0 g (“w” grams) of oil by use of an analytical balance.

NoteNote

Note: to dose exactly 4.6 ml of the oil sample:

NoteNote (a) push the plunger completely into the syringe, (b) insert the tip into the oil sample, (c) pull the plunger up until the lower edge of the seal

is exactly on the 5 mL mark,

(d) take out the syringe and clean the outside of the

syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them,

(e) then, keeping the syringe in vertical position above

the oil sample, push the plunger down into the syringe, till the 4.6 ml sign,

(e) be sure that no drops are hanging on the tip of

the syringe, if so eliminate them,

(g) then, keeping the syringe in vertical position above

the (open) bottle of Organic Solvent, push the plunger completely down into the syringe. Now the exact amount of 4.6 mL has been added to the bottle.

as soon as the solution is persistently

light pink.

• Remove the cap and add the oil sample to the Organic Solvent bottle. Replace the cap.

• Place the sample on the magnetic stirrer and stir gently until all the oil sample is dissolved in the Organic Solvent reagent.

• Using the 1.0 ml graduated syringe with tip, take

1.0 ml of HI 3897-0 Titrant Solution reagent. NoteNote

Note: to dose exactly 1.0 ml of the Titrant Solution

NoteNote reagent:

(a) push the plunger completely into the syringe, (b) insert tip into HI 3897-0 Titrant Solution reagent, (c) pull the plunger out until the lower edge of the

plunger seal is on the 0 mL mark of the syringe,

(d) take out the syringe and clean the outside of the

syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them.

• Remove the cap.

• Hold the syringe in vertical position above the bottle. Slowly add the titration solution dropwise, waiting for the solution to be stirred after each drop.

• Continue adding titration solution until the solution in the bottle changes from yellow-green to pink.

NoteNote

Note: stop adding the HI 3897-0 Titrant Solution

NoteNote reagent to the bottle pink.

• Read the milliliters of titration solution from the syringe scale and obtain the acidity of the extra virgin olive oil sample as follow:

- If 4.6 ml of oil sample was dosed to the bottle, then the acidity is :

ml of titrant = % oil acidity

[i.e.: 0.5 ml = 0.5% oil acidity]

as soon as the solution is persistently

CAUTION! Carefully open the bottle of

HI 3897-010 Organic Solvent Replacement Kit , because the bottle may be under pressure!

Store the bottle tightly closed in a cool, ventilated place.

• Transfer 40 ml of reagent from the Replacement Kit bottle to an empty Organic Solvent bottle, to the 40 ml sign.

NotaNota

Nota: the empty Organic Solvent bottle must be clean

NotaNota and dry.

• Add a magnetic bar and cap the bottle tightly.

• To perform a new test, proceed as described in the “Instructions”.

AccessoriesReplacement Kit Instructions

HI 3897-010 replacement kit (10 tests) HI 740053 100 ml graduated glass bottle (10 pcs) C215-00300 5 ml graduated syringe HI 740142 1 ml graduated syringe HI 740143 1 ml graduated syringe (6 pcs) HI 740144 tips for 1 ml graduated syringe (6 pcs) HI 180IMD magnetic stirrer HI 731319 magnetic bar (10 pcs)

Bibliography

Commission Regulation (EEC) No 2568/91 and following updates.

Health and Safety data Sheets

The chemicals contained in this test kit may be hazardous if improperly handled. Read Health and Safety Data Sheets before performing the test.

- If “w” grams of oil sample was weighted and added to the bottle, then the acidity is :

% oil acidity