Instruction Manual
HI 3873
Nitrite
Test Kit
SPECIFICATIONS
Range 0 to 1.0 mg/L (ppm) as NO
Smallest Increment 0.2 mg/L (ppm) NO
Analysis Method Colorimetric
Sample Size 10 mL
Number of Tests 100
Case Dimensions 230x59x70 mm (9.0x2.3x2.8")
Shipping Weight 169 g (6.0 oz.)
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INSTRUCTIONS
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
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• Fill the glass cuvet with 10 mL of the
sample, up to the mark.
• Add 1 packet of HI 3873-0 reagent.
• It is better to match the color with a white sheet at
about 10 cm behind the comparator.
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Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instructions carefully before using the
chemical test kit. It will provide you with the necessary
information for correct use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable damage, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
HI 3873-0 Reagent, packets (100 pcs);
•
• 1 glass cuvet;
1 color comparator cube.
•
Note: Any damaged or defective item must be returned in
its original packing materials.
SIGNIFICANCE AND USE
Nitrites are intermediate oxidation state of nitrogen (in the
oxidation of ammonia to nitrate or in the reduction of
nitrate). Such oxidation/reduction may occur in wastewater
of treatment plants and in natural waters during the
biological decomposition of nitrogen-compounds. In small
quantities it can cause methaemoglobinemia among infants. Conversely, high levels are used to inhibit corrosion in
cooling towers. Nitrosation reactions of nitrites can yield
organic nitrosamines, which are known to be carcinogenic.
Note: mg/L is equivalent to ppm (parts per million).
CHEMICAL REACTION
Nitrites react with chromotropic acid reagent to form a pink
tint in the sample. The amount of color developed is
proportional to the concentration of nitrite present in the
aqueous sample.
ISTR3873 12/99 PRINTED IN ITALY
• Replace the cap and shake
gently until the powder is
dissolved. A deposit may remain but it will not affect
the reading.
• Wait for 6 minutes to allow the color to develop. Remove
the cap and fill the color comparator cube with 5 mL of
the treated sample (to the mark).
• Determine which color matches the
solution in the cube and record the
result in mg/L (or ppm) of Nitritenitrogen.
• To convert the reading to mg/L of Nitrite (NO
multiply the reading by a factor of 3.29.
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REFERENCES
Adaptation of the EPA approved method 354.1.
HEALTH AND SAFETY
The chemicals contained in this kit may be hazardous if
improperly handled. Read Health and Safety Data Sheet
before performing this test.
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