Hamamatsu Photonics C11440-22CU, C11440-22CU01 Instruction Manual

•

Digital Camera

C11440-22CU/C11440-22CU01

Instruction manual

Thank you for your purchase

Follow the safety precautions in Chapter 1 in order to avoid pe rsonal injury and damage to property when using this camera. Be sure to read this Instruction manual beforehand in order to use the digital camera correctly. The manual describes the correct method of handing the camera and provides cautions in order to avoid accidents.

• After reading, keep the manual where it can be referred to at any time.

Ver.1.4

July 2014

HAMAMATSU PHOTONICS K.K.

95900301-04

~ Blank page ~

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Improper handling of the camera without obse rv i ng these warnings could lead to serious injury to the user and even death.

Improper handling of the camera without observing these cautions could lead to personal injury to the user or damage to property.

Note

This symbol indicates a note t o help you get the best performance from the camera. observe one of these notes might impair the perf ormance of the camera.

1. SAFETY PRECAUTIONS

1-1 INDICATION OF THE SYMBOLS

The following symbols can be found on this camera:

Direct current

Alternating current

1-2 CLASSIFICATION OF WARNING

We have classified the warnings symbols that appear i n this instruction manual and on t he camera as follows for your convenience. Make sure that you fully understand them and obey the instructions they contain.

Read the contents of the note carefully to ensure correct and safe use. Failure to

This symbol indicates a cautionary item that should be obeyed when handling the camera. Read the contents carefully to ens ure correct and safe use.

This symbol indicates an action that is forbidden. Read the contents carefully and be sure to obey them.

This symbol indicates a compulsory action or instruction. Read the contents carefully and be sure to obey them.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Power supply

Cables Power supply cord

Do not attempt to dismantle or modify the camera

Do not insert a foreign substance into the camera

If an abnormality occurs

Use the camera with the voltage indicated on the rating sticker. Using a different voltage can damage the camera and lead to fire or electric sh o ck.

Be careful not to place heavy objects on cables or bend it excessively. Doing so can damage the cable and lead to fire or electric shock.

Use the accessory of the AC adaptor whe n thi s camera is used.

Do not touch the plug with wet hand. Doing so can lead to electric shock.

Doing so can also lead to damage and even injury, as some internal components become very hot. Only touch parts as indicated in this manual.

Do not allow foreign objects such as combustible substances, metal objects or water to get inside the camera. They can damage the camera and lead to fire or electric shock.

Such as the image suddenly disappearing or a strange noise, smell or see smoke coming from the camera, stop the power supply immediately and contact Hamamatsu subsidiary or local distributor. Never attempt to repair the camera yourself.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

AC adaptor

Doing so can lead to fire or electric shock.

Connecting and disconnecting cables

supply of the peripheral device before

Fixed the camera Lenses (C11440-22CU) Shipping precautions

Strong impact

At the water cooling

Operating environment

When unplugging the AC adaptor, always pull by the plug, not the cord.

Remove the AC adaptor from the outlet when not using the camera for long periods of time. Doing so can damage the cable and lead to fire or electric shock.

Always turn off the power connecting and disconnecting cables.

When fitting the camera to a tripod or other fixture, use the optional base plate. Be careful that the fitting screw does not enter more than 8 mm from the surface of the base plate. Screwing it in excessively can impair normal operation.

Be careful not to screw the lens more than 7 mm onto the C-mount of the camera. Doing so can scratch the protective glass. (Some wide-angle lenses in particular can have a thread of 7 mm or more. )

When transporting the camera by truck, ship, airplane, etc., wrap it securely in packaging material or something si milar.

Do not subject the camera to strong shocks by dropping it, for example. Doing so can damage the camera.

Be careful water does not splash on the camera. Cut off the power supply of the circulating water cooler and the camera when you remov e and install the cooling water hoses.

This system is designed and tested for use in an industrial environment. If this system is used in residential areas, EMI (electro-magnetic interference) may occur. This system must not be used in residential areas.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Disposal

with applicable regulations regarding waste disposal and correctly

Cooling water

Condensation

f ambient temperature and ambient humidity

When disposing of the camera, take appropriate measures in compliance dispose of it yourself, or entrust disposal to a licensed industrial waste

disposal company. your country, state, region or province to ensure the camera is disposed of legally and correctly.

It is recommended to use soft water (except deionized water) for cooling water. Follow instruction manual which is attached to your circulating water cooler for an appropriate temperature range of cooling water. If you plan on using water other than soft water as recommended for example antifreeze etc, please refer to description of cooling water which is written in 12. [Maintenance] or contact Hamamatsu subsidiary or local distributor.

In any case, be sure to comply with the regulations in

At the Water-cooling, i become high, condensation will take place easily. Use the camera under the environment where condensation will not take place referring to the following graph.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Camera: C11440-22CU or C11440-22CU01

AC adaptor

Power supply cord for AC adaptor

Lens mount cap (attached to the camera)

C11440-22CU/C11440-22CU01 Before Use (Booklet)

C11440-22CU/C11440-22CU01 Instruction manual (CD-ROM)

QC sheet

Circulating water cooler

C3142-07

Cooling water hose (2 hoses)

A10788-04

SMA-BNC cable

A12106-05

SMA-SMA cable

A12107-05

Camera Link interface cable

A11255-05

USB 3.0 interface cable

A12467-03

USB 3.0 interface board

M9982-21

Adjuster pole for C11440-22C

A11185-01

Base plate for C11440-22C

A11186-01

Camera Link interface board

M9982-20

Note

• The cable listed in option is highly recommended for use with the c amera. The camera

Note

special software included.

Note

2. CHECK THE CONTENTS OF PACKAGE

When you open the package, check that the f ollowing items are incl uded before use. If the contents are incorrect, insufficient or damaged in any wa y, contact your local dealer wi thout attempting to operate the camera.

[Option]

system may not confirm to CE marking regulation if other type of cable is used with.

• The cooling is set as air cooling at factory setting. It is possible to change the cooling setting from Air cooling to Water cooling by the

• Handle the circulating water cooler and the cooling water according to an instruction manual of the circulating water cooler.

• If you use the adjuster pole and the base plate, see each installation manual.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

When the ambient temperature for using t his system might fall be low 0 °C or rise

• Where there is a lot of dust.

To prevent overheating in the camera’s interior, do not wrap the camera

exhaust vents when setting up.

3. INSTALLATION

Avoid using or storing this camera in the following places

•

above +40 °C.

How to place the camera (when the camera is placed on a table)

• When the ambient temperature for st oring this system might fall below -10 °C or

rise above +50 °C.

• Where the temperature varies extr em ely.

• In direct sunlight or near a heater.

• Where the humidity is 70 % or more or where there is dripping water.

• Close to a strong source of magnetism or ratio waves.

• Where there is vibration.

• Where it might come into contact with corrosive gases (such as chlorine or

fluorine).

Place the camera the water connectors to be lateral side.

Do not place the camera the rear panel of the camera, which connectors are located, to be at the bottom. (Do not block ventilation openings.)

Do not block ventilation openings

in cloth or other material, or in any way allow the camera’s ventilation ports to become blocked. If the cam era is being operated in a n enclosed environment, ensure clearance of at least 2 cm from both the intake and

Weight of the camera

Be careful not to drop off the camera or not drop underfoot when making it move because it is approx. 2 kg.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

CCoonntteennttss

1. SAFETY PRECAUTIONS ............................................................................ 1

1-1 INDICATION OF THE SYMBOLS ....................................................................... 1

1-2 CLASSIFICATION OF WARNING ...................................................................... 1

2. CHECK THE CONTENTS OF PACKAGE ................................................... 5

3. INSTALLATION ........................................................................................... 6

4. OVERVIEW .................................................................................................. 9

5. FEATURES .................................................................................................. 9

6. NAME AND FUNCTION OF THE PARTS ................................................. 12

7. CONNECTION ........................................................................................... 15

8. WATER COOLING .................................................................................... 18

8-1 CAUTIONS ........................................................................................................ 18

8-2 CONNECTION OF WATER COOLING HOSES................................................ 20

8-3 DISCONNECTION OF WATER COOLING HOSES .......................................... 21

9. OPERATION .............................................................................................. 22

9-1 PRECAUTIONS ................................................................................................. 22

9-2 PREPARATION FOR IMAGING........................................................................ 23

9-2-1 WHEN USING AIR-COOLING .............................................................................................. 23

9-2-2 WHEN USING WATER-COOLING ....................................................................................... 23

9-3 END OF IMAGING ............................................................................................. 23

10. DESCRIPTION OF VARIOUS FUNCTIONS ............................................. 24

10-1 THEORY OF CMOS IMAGE SENSOR ............................................................. 24

10-2 NORMAL AREA MODE .................................................................................... 26

10-2-1 READOUT METHOD (SCAN MODE) .................................................................................. 26

10-2-2 FRAME RATE CALCULATION ............................................................................................. 27

10-2-3 CONFIGURING EXPOSURE TIME ...................................................................................... 31

10-2-4 FREE RUNNING MODE ...................................................................................................... 32

10-2-5 EXTERNAL TRIGGER MODE .............................................................................................. 34

10-2-6 TRIGGER OUTPUT ............................................................................................................. 39

10-2-7 SLOW SCAN ........................................................................................................................ 41

10-2-8 GLOBAL RESET .................................................................................................................. 42

10-3 LIGHT SHEET MODE ....................................................................................... 45

10-3-1 READ-OUT DIRECTION ...................................................................................................... 45

10-3-2 FRAME RATE CALCULATION ............................................................................................. 46

10-3-3 READ-OUT TIME OF THE HORIZONTAL LINE ................................................................... 46

10-3-4 TIMING DIAGRAM ............................................................................................................... 47

10-3-5 PROGRAMMABLE TIMING OUTPUT .................................................................................. 49

10-4 REAL-TIME CORRECTION FUNCTIONS ........................................................ 50

11. PRECAUTION WHEN USING FL-400 ....................................................... 51

12. MAINTENANCE ........................................................................................ 52

12-1 CARE ................................................................................................................. 52

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

12-2 INFORMATION ON COOLING WATER FOR T HE CIRCULATING WATER

COOLER ........................................................................................................... 53

12-2-1 WHEN USING COOLING WA TER OTHER THAN RECOMMENDED ................................. 53

13. TROUBLESHOOTING CHECKLIST ......................................................... 54

13-1 IMAGE IS NOT TRANSFERRED ...................................................................... 54

13-2 ALTHOUGH IMAGES ARE TRANSFFERED ................................................... 54

14. SPECIFICATIONS ..................................................................................... 55

14-1 CAMERA SPECIFICATIONS ............................................................................ 55

14-2 CONDENSATION .............................................................................................. 58

14-3 SPECTRAL RESPONSE CHARACTERISTICS ............................................... 59

14-4 INTERFACE SPECIFICATIONS ....................................................................... 60

14-4-1 CAMERA LINK INTERFACE ................................................................................................ 60

14-4-2 OUTPUT TIMING SPECIFICATIONS ................................................................................... 63

15. DIMENSIONAL OUTLINES ....................................................................... 67

15-1 C11440-22CU .................................................................................................... 67

15-2 C11440-22CU01 ................................................................................................ 68

16. WARRANTY .............................................................................................. 69

17. CONTACT INFORMATION ....................................................................... 70

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

4. OVERVIEW

C11440-22CU/C11440-22CU01 is equipped with the new scientific image sensor FL-400, an advanced CMOS device that realizes the multiple benefits of high resolution, high readout speed, and low noise all at once. The camera provides 4.0 megapixels resolution at 100 frame/s (and up to 25 655 frame/s by sub-array readout) while achieving 1.0 electrons (median) 1.6 electrons (r.m.s) readout noise performance. Moreover, the camera delivers high sensitivity through i ts on-chip micro lens, 37 000:1 high dynamic range that make the camera suitable f or almost any scientific application from bright field imaging to low-light fluorescence imaging across a wide spectral range. Various external trigger funct ions and timing output functions ensure proper timing control with peripheral equipment to cover a wide range of applications. The camera is the new scientific digital camera for life science microscopy, sem iconductor inspection, x-ray scintillator readout or i ndustrial imaging.

5. FEATURES

(1) Readout noise

In the camera, the pixel amplifier is optimized: it has high gain from optimizing the semiconductor process, and the difference among pixel amplifiers are greatly minimized. In addition, there is on-chip C DS (correlat ed doubl e sam pling) circuit , whi ch plays an important role in achieving low noise. Moreover, the sensor features a split readout scheme in which the top and bottom halves of the sensor are readout independently, and the data of each horizontal line is read by 2 lines of column amplifier and A/D in the top and the bottom in parallel and simult aneou sly. As a result, it achieves very fast readout speed while keeping very good l ow-noise performance. The camera has lower readout noise (1.0 electrons (median), 1.6 electrons (r.m.s)) than the conventional cooled CCD camera. Moreover, high-speed readout (100 frame/s with 2048 pixels × 2048 pixels) with very low readout noise, which was impossible, can now be achieved.

(2) Cooling structure

In the camera, the FL-400 sensor is cooled down by the peltier element t o suppress the dark current. If FL-400 is exposed to the atmosphere, condensation of the moisture from the air might occur. However the camera has a special hermetic chamber structure to isolate the sensor from the atmosphere, and the chamber is filled with nitrogen gas.

(3) Pixel number and pixel size

The FL-400 sensor has 6.5 µm x 6.5 µm pixel sizes that is equivalent to conventional CCD image sensor (2/3 inch, 1.3 megapixels). Also, the camera can observe a wider field of view because the pixel number is about 3 times that of the conventional CCD image sensor (2/3 inch, 1.3 megapixels)

(4) Readout method

The camera has a variety of readout modes. In addit ion to full resolution readout mode, sub-array readout and binning readout are supported.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

(5) Frame rate (readout speed)

The FL-400 realizes both low noise (1.0 electrons (median) 1.6 electrons (r.m.s)) and high speed readout (100 frame/s with 2048 pixels x 2048 pixels) simultaneously, by a split readout scheme in which the top and the bottom halves of the sensor are readout independently, and the data of each horizontal line is read by 2 lines of column amplifier and A/D in the top and the bottom in parallel and simultaneously.

(6) Real-time correction functions

When using the camera, there is a case that shading caused by uneven illumination or optics is not negligible in the image. Also, there are a few pixels in FL-400 that have slightly higher readout noise performance compared to surrounding pixels. For those cases, the camera has real-time offset level, shading and defective pixel correction features to further improve image quality. The correction is performed in real-time without sacrificing the readout speed at all.

(7) Interface

This camera has both Camera Link and USB 3.0 interface.

■ Camera Link Interface

Camera Link interface is able to transfer large volumes of data. It can transfer 4 megapixels image with 100 frame/s. In order to realize such large volume data transfer, the camera uses Camera Link "Full Configuration Deca Mode" which is an expanded version of Camera Link "Full Configuration". It enables to transfer maximum 85 MHz x 10 Taps (8 bit) image data to computer as fast as 100 frame/s. In order to use this interface, a Camera Link interface board which supports "Full Configuration Deca Mode" is required.

■ USB 3.0 Interface

USB 3.0 interface is able to transfer 4 megapixels image with 30 frame/s. It is versatile interface which is suitable to use when fast data transfer is not required. This interface does not require a Camera Link interface board. It transfers image with moderate transfer speed.

• Do not connect Camera Link and USB 3.0 interface simultaneously.

• When a connection interface is changed from Camera Link to USB 3.0, and vice versa, the

application software must be closed and the camera must be turned off.

(8) Camera readout modes

The camera has two scan speed in Normal area mode, and two readout direction in Light sheet mode.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

(9) Camera operation modes

The camera has three operation modes: 1) the free running mode, in which the exposure and readout timing are controlled by the internal microprocessor, and 2) the external trigger mode, in which the exposure and readout timing are decided by an external trigger. 3) the start trigger mode is used to start operating the camera by a trigger input for a continuous imaging.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

6. NAME AND FUNCTION OF THE PARTS

(1) C11440-22CU

②

① ④ ③ ⑤ ⑥

⑪ ⑦ ⑧ ⑨ ⑩

⑫

(2) C11440-22CU01

②

① ④ ③ ⑤ ⑥

⑪ ⑦ ⑧ ⑨ ⑩

⑫

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Place the camera the water connectors to be later al side. Do not place bottom (Do not block ventilation openings.).

Note

• The depth of the C-mount is 7 mm. Screwing in the mount too far

can scratch the glass surface.

Note

Lighting color

Status of power distribution

Turn off (no color)

Power off

Green (Blinking)

Initialization

Green (lighting)

Power on

Orange (lighting)

Data transfer

Red (lighting)

Heat up

When the camera heats up, stop operation and unplug the AC adaptor immediately.

•

the rear panel of the camera, which co nnectors are locate d, to be at the

① Lens mount

C11440-22CU can be attached to C-mount lens or an optics system. C11440-22CU01 can be attached to F-mount lens or an optics system.

② WATER connector [WATE R] (at Water-cooling)

It connects the camera and the circulating water cooler with the cooling water hoses. The insert position of WATER IN/OUT is not specified.

• Please see 8. [Water cooling] for details .

③ Camera Link interface connector [DIGITAL OUT 1]

This is connected to the Camera Link inter face connector 1 on the computer.

④ Camera Link interface connector [DIGITAL OUT 2]

This is connected to the Camera Link interface connector 2 on the computer.

⑤ Trigger input connector [EXT.TRIG]

This is used when the camera is being operated using external synchroniz ation. Input is 3.3 V LVCMOS level, and input impedance is 10 kΩ. When an external trigger is input, the trigger is activated at the falling or rising e dge of the signal. (You can choose external trigger polarity between Negative and Pos i tive.)

⑥ Timing out connector 1,2,3 [TIMING 1,2,3]

This is used when peripheral device(s) require synchronization with the camera. Output is 3.3 V LVCMOS level, and it is output though Output impedance is 33 Ω.

• Determine termination according to cable length and so on.

BUS TRANSCEIVER IC SN74AVC8T245.

⑦ STATUS lamp [STATUS]

The LED indicates status of camera.

•

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

⑧ Power switch [POWER]

The power is turned on/off. When the power switch is set to "ON", the camera turns on and starts initialization and the lamp blinks in green. When the initialization is completed , the lamp color stays in green. When the camera transfers data and the lamp c olor is orange. When the power switch is set to "OFF", the camera ret urns t o t he power off state and the lamp turns off.

⑨ DC power input connector [DC IN]

This is the power supply terminal. Us e t he ac ces sor y AC adaptor.

⑩ USB 3.0 interface connector [USB 3.0]

This is connected to the USB 3.0 interface connector on the computer.

• Do not connect Camera Link and USB 3.0 interface simultaneously.

• When a connection interface is changed from Camera Link to US B 3.0, and vice

versa, the application software must be closed and the camera must be turned off.

⑪ Air outlet

This is the outlet for the heat ventilation.

• To prevent overheating inside the camera, do not wrap the camera in cloth or other material, or block the camera’s ventilation.

• If the camera is being operated in an enclosed environment, ensure to keep clearance at least 2 cm from both intake and exhaust vents when setting up.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

bottom (Do not block ventilation openings.).

7. CONNECTION

• When you connect cables, turn off the power supply of the camera and the peripheral devices.

• If you use the adjuster pole and the base plate, see each installation manual.

• Place the camera the water connectors to be later al side. Do not place

the rear panel of the camera, which connec tors are located, to be at the

Refer to the figure when connecting the various cables .

 Overall connection

Camera (Rear)

② Cooling water hose (2 hoses)

Interface cable

Computer

Interface board

① AC adaptor

Circulating water cooler

Figure 7-1

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

■

Connection to Camera Link

Connection to USB 3.0

Camera (Rear)

③ ④ Camera Link Camera Link interface cable 1 interface cable 2

Computer

① AC adaptor

To connector 2

To connector 1

Camera Link interface board

Camera (Rear)

AC adaptor ①

Computer

USB 3.0 interface board

Figure 7-2 Figure 7-3

⑤ USB 3.0

interface cable

• Do not connect Camera Link and USB 3.0 interface simultaneously.

• When a connection interface is changed from Camera Link to US B 3.0, and vice

versa, the application software must be closed and the camera must be turned off.

① AC adaptor

This is the cord to supply a power supply. Use the accessory AC adaptor.

② Cooling water hose (at Water-cooling: Option)

It connects the camera and circulating water cooler. The insert position of WATER IN/OUT on the camera WATER connector is not specified.

• Please see 8. [Water cooling] for details .

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

• Hamamatsu recommends A11255-05 optional Camera Link interface cable for

Note

• Hamamatsu recommends A12467-03 optional USB 3.0 interface cabl e for this

cable may not fulfill the EMC directive requirements.

③ Camera Link interface cable 1 (Optio n)

This is the cable to connect the Camera Link interface connector 1 of the camera and the Camera Link interface connector 1 on the computer.

④ Camera Link interface cable 2 (Optio n)

This is the cable to connect the Camera Link interface connector 2 of the camera and the Camera Link interface connector 2 on the computer.

this camera. The camera complies with EMC direction with using A11255-05 Camera Link interface cable. Be careful that the camera with other interface cable may not fulfill the EMC directive requirements.

⑤ USB 3.0 interface cable (Option)

This is the cable to connect the USB 3.0 interface c onnector of the camera and the US B 3.0 interface connector on the computer.

camera. The camera complies with EMC direction with using A12467-03 Camera Link interface cable. Be careful that the camera with other interface

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Improper handling of the camera without observing these cautions could lead to personal injury to the user or damage to property.

8. WATER COOLING

•

8-1 CAUTIONS

(1) Cooling water

It is recommended to use soft water (exc ept deionized water) for cooling water. If you plan on using water other than soft water as recommended for example antifreeze etc, please refer to description of cooling water which is written in 12. [Maintenance] or contact Hamamatsu subsidiary or local distributor.

(2) Recommendation ambient temperature

Hamamatsu recommends 20 °C for Circ ul ating water temperature. For the appropriate temperature range o f the cooling water, confirm with the instruction manual of your circulating water cooler.

(3) Condensation

Use the camera under the environment where condensation will not take place referring to the following graph.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

• Follow the instruction in Section 8-2 [CONNECTION OF WATER COOLING HOSES] to connect / disconnect the hose.

Blue hose

Gray hose

Joint

Button

(4) Handling of the circulating water cooler

Handle the circulating water cooler and the cooling water according to an instruction manual of the circulating water cooler. Proper performance may not be achievable if a non-recommended circulating water cooler is used.

(5) Start water cooling and water cooping in operation

• Confirm the water is flowing before starting the camera cooling and that the camera

does cool.

• Keep 1.0 L/min flow rate for water circulation.

• Do not stop coolant while the camera is working.

(6) Cooling water hose

The hose has a blue hose (Internal diameter: 4 mm / External diameter: 6 mm) and a gray hose (Internal diameter: 8 mm / External diam eter: 13.5 mm). (Figure 8-1) If the hose size on circulating water cooler is the same as blue hose, remove gray hose from the joint part. The gray hose can be removed when blue hose is pulled with pushing the button of the joint on gray hose. (Figure 8-2)

Figure 8-1 Figure 8-2

(7) Connection of the cooling water hose

COOLING HOSES] and Section 8-3 [DISCONNECTION OF WATER

• Stop water circulation when connecting / disconnecting the hose, and turn off the

power of the camera and the circulating water cooler.

• Confirm that cooling water stops.

• Prepare water absorption sheet (such as W aste, Towel or so) and catch pan in order

to avoid water drop or water splash.

(8) Deterioration of the cooling water hose

Replace the water hose with a new one whenever it cannot keep the Keep 1.0 L/min flow rate for water circulation due to the hose deteri oration.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Stop the circulating water cooler whe n the water flo w i s abnorm al or water drop or splash is found.

Water cooling hose

WATER connector

8-2 CONNECTION OF WATER COOLING HOSES

Figure 8-3

(1) Place the camera on the stable table. (2) Connect water cooli ng hose into the WATER connector on the camera.

• Insert the hose fully into the WATER connector on the camera. (as shown in Figure

8-3)

• Confirm the hose stops at it.

(3) Set the camera onto a microscope (If the camera is used on the microscope).

If it is easy to connect the hose onto the camera after the camera is set onto the microscope then it is OK to connect the hose after the camera is set on the microscope.

(4) Connect the hose onto the circulating water cooler.

Follow the instruction on the circulating water cooler when you connect the hose onto the circulating water cooler.

(5) Turn on the circulating water cooler and confirm the cooling water is flowing normally.

•

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Cooling water may be lef t insid e the cam era even af ter hos es are rem oved. not to splash water onto the camera.

Button

8-3 DISCONNECTION OF WATER COOLING HOSES

• Remove the water cooling hoses only when it is necessary to remove.

(1) Turn off the camera power and all peripheral devices including circulati ng wat er cooler. (2) Remove the hose on circ ulating water cooler side.

Follow the instruction on the circulating water cooler when you disconnect the hose from the circulating water cooler.

(3) Remove water or water drop inside the hose and the camera by air.

• Blow Air from one side of hose. Prepare water absorption sheet (such as Waste,

Towel or so) and catch pan on another side of hose in order to avoid water drop or water splash.

• Blow Air until no water drop come out.

(4) Remove the camera fro m t he mi croscope (if the camera is used on the microscope).

It is not necessary to remove the camera from the microscope if it is possible to remove the hoses from the camera as it is.

(5) Place the camera on the stable table.

Put the lens cap on to protect the sensor.

(6) Change the WATER connector direction to be downward.

Prepare water absorption sheet (such as Waste, Towel or so) and catch pan.

(7) Remove hoses one by one, and wipe water.

Disconnect hoses with pushing button while being careful not to splash water.

•

In such case, remove water inside by blow ing air from connec tors. Be careful

Figure 8-4

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

Cooling method

Detail

Air-cooling mode (Default)

The heated side of a peltier element is cooled by a fan inside the cooling is started.

Circulating water cooler (Optiona l) is used for cooling the heated side

(Please refer to 8 [WA TER COOLING] for instruction of water-cooling.)

9. OPERATION

9-1 PRECAUTIONS

Be careful of the following when you operate the camera.

(1) Cooling method

Cooling of this equipment is done using a Peltier elem ent. With a P eltier element , when current is supplied, one surface is cooled, and the other surface is heated. The FL-400 sensor is positioned on the cooled side, and cooling is done by discharging the heat from the heated surface. The camera has two cooling modes, Air-cooling mode and Water-cooling mode. Cooling mode can be changed by software which is called, “DCAM Configurator”. After cooling mode was changed, the camera memorizes the last setting as the default setting for cooling. The present cooling mode set-up of this camera can be checked using “DCAM Configurator”.

• A software “DCAM Configurator " is in a CD-ROM of DCAM software.

(Forced air-cooled)

Water-cooling mode

camera. When the camera is turned on, the fan starts rotating and

of a peltier element. Cooling does not start just turning on the camera. Cooling water circulation must be started before start operating the camera in water-cooling. A fan inside the camera does not rotate.

• Do not switch to water-cooling mode when water-cooling is unnecessary.

(2) Ambient temperature

The recommended ambient temperature for cam era operation is 20 °C. Both water-cooling or air-cooling are available as cooling method, FL-400 sensor cooling temperature is more stable under water cooling operation.

(3) Protection circuit

A double protection circuit protects this camera’s thermoelectric cooling device. If the heat dissipater becomes abnormally hot, the protection circuit sets off a buzzer alarm and stopping current supply to Peltier element simultaneously. When the protection circuit is activated immediately turn off the power switch. Then investigate cause and remove the cause of the overheating and restart the camera.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

• Please refer to the manual of application software for ON/ OFF of th e cooling switch of

a camera.

9-2 PREPARATION FOR IMAGING

Use the following procedure when start operating the camera.

• When you connect cables, turn off the power supply of the camera and the peripheral devices.

• Please change cooling method (cooling mode) if needed. T he present c ooling mode set-up o f

this camera can be checked using DCAM Configurator.

9-2-1 WHEN USING AIR-COOLING

(1) Connect the equipment as shown in Figure 7-1 before operating of the camera. (2) Turn on the camera. (3) Check cooling fan is operating properly and air is circulating.

9-2-2 WHEN USING WATER-COOLING

(1) Connect the equipment as shown in Figure 7-1 before operating of the camera. (2) Turn on the camera. (3) Turn on the circulati ng water cooler. (4) Check cooling water is circulating suitably. (5) Turn on the cooling switch of the camera from application software.

9-3 END OF IMAGING

Follow the procedure below when imaging is finished.

(1) End the imaging or transmission of image data with the application softwa re. (2) Turn off the circulating water cooler. (In the case of water-cooling) (3) Turn off the camera and peripheral devices.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

10. DE S CRIPTION OF VARIOUS FUNCTIONS

10-1 THEORY OF CMOS IMAGE SENSOR

The pixel of a CMOS image sensor is composed of the photodiode and the amplifi er that converts the charge into voltage. Entered light is converted to charge and converted to voltage in the pixel. The voltage of ea ch pixel is out put by switching t he switch one by o ne. (Figure 10-1) The FL-400 scientific CMOS image sensor used in this camera has an on-chip CDS (correlated double sampling) circuit, which plays an important role in achieving low noise. In addition, the FL-400 realizes both low noise and high speed readout simultaneous ly, by a split readout scheme in which the top and the bottom halves of the sensor are readout independently, and the data of each horizontal line is read by 2 lines of column amplifier and A/D in the top and the bottom in parallel and simultaneously.

Figure 10-1 Structure of the FL-400

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

The exposure and the readout method of FL-400 is rolling shutter. In the rolling shutter, the exposure and readout are done line by line. Therefore, the exposure timing is different on one screen. (Figure 10-2) But even if the object moves during the exposure, the affect of rolling shutter is very small.

Figure 10-2 Readout timing of Rolling shutter

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Settings

Horizontal

Vertical

Size

Position

Size

Position

Note

• Please refer to 10-2-22-2 [FRAME RATE CALCULATION] about the frame rate of each

readout mode.

10-2 NORMAL AREA MODE

10-2-1 READOUT METHOD (SCAN MODE)

(1) Readout direction

The camera readout the image sensor from the center line to the top and from the center line to the bottom simultaneously (center line is depicted in red line in the diagram).

Figure 10-3 Normal area mode readout direction

(2) Normal readout

Perform charge readout from camera individually for all pixels.

(3) Binning readout

With this camera, 2×2 binning readout and 4×4 binning are available by adding the signal of adjacent pixels in the digital domain, Binning readout is a method for achieving high sensitivity in exchange for losing resolution.

(4) Sub-array readout

Sub-array readout is a procedure only a region of interest is scanned. It is possible to increase the frame rate by reducing the number of vertical lines scanned. When a target area is placed in the center of the screen, sub-array readout can perform the fastest readout. In sub-array readout, binning configuration is enabled.

Size and a position of the readout area can be confi gured a ccordi ng to t he table below.

Interface Binning

Camera Link

Regardless of binning ― ― 4 lines steps 1×1 (Normal readout) 512 pixels 32 pixels 8 lines 4 lines

USB 3.0

2×2 binning readout 256 pixels 16 pixels 4 lines 2 lines 4×4 binning readout 128 pixels 8 pixels 2 lines 1 lines

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Horizontal

Vertical

Frame rate

(frame/s)

Free running mode

1/(Vn/2×1H)

2048

100

1024

200

512

400

256

801

128

1603

3206

25 655

External trigger mode

1/(Vn/2×1H+Exp1+1H×9)

2048

1024

164

512

278

256

427

128

582

712

884

External trigger mode

1/(Vn/2×1H+1H×18)

2048

1024

193

512

374

256

702

128

1251

2052

4664

External trigger mode

1/(Vn/2×1H+1H×5)

2048

1024

198

512

393

256

771

128

1487

2773

11 402

10-2-2 FRAME RATE CALCULATION

10-2-2-1 At Standard Scan

(1) Camera Link

Vn = Number of vertical line (The center of the set area is the middle of the sen s or.) Exp1 = 1.004 ms to 10 s

1H = 9.74436 × 10

• The Exp1 value must be input to the calculation formula below in units of seconds.

[Calculation formula, Value of Frame rate ]

-6

Calculation formula

(Edge trigger / Level trigger)

(Synchronous trigger readout)

(Fast Synchronous trigger readout)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Horizontal

Vertical

Free running mode

1024

1536

2048

1×1

512 ―

2×2 ― ―

4×4 ― ―

External trigger mode

1024

1536

2048

1024

1536

2048

1×1

512 ―

2×2 ― ―

4×4 ― ―

External trigger mode

1024

1536

2048

1024

1536

2048

1×1

512 ―

2×2 ― ―

4×4 ― ―

External trigger mode

1024

1536

2048

1024

1536

2048

1×1

512 ―

2×2 ― ―

4×4 ― ―

(2) USB 3.0

Vn = Number of vertical line (The center of the set area is the middle of the sen s or.) Exp1 = 1.004 ms to 10 s Exp2 = 1.05 ms to 10 s 1H = 9.74436 × 10 int () = The decimal p oi nt is rounded down.

• The Exp1 and Exp2 values must be input to the calculation formula below in units of seconds.

[Calculation formula]

-6

Binning

1×1

(Edge trigger)

(Level trigger)

1×1

Calculation formula

― 1/ (int(Vn/2048/30/1H)×1H)

1/ (int(Vn/2048/100/1H)×1H)

112≦Vn≦2048 1/ (int(Vn/2048/30/1H)×1H)

8≦Vn≦104

1/ (Vn/2×1H+1H×9+Exp1)

112≦Vn≦2048 1/ (int(Vn/2048/30/1H)×1H)

8≦Vn≦104

(Synchronous trigger readout)

1×1

24≦Vn≦2048 1/ (int(Vn/2048/30/1H)×1H)

8≦Vn≦16

1/ (Vn/2×1H+1H×4+Exp2)

1/(Vn/2×1H+1H×18)

Binning

1×1 (Normal readout)

2×2 / 4×4

Horizontal

Vertical

Free running mode

2048

100

1024

200

512

120

400

256

240

801

128

481

1603

968

3206

7894

25 655

External trigger mode

2048

1024

164

512

120

278

256

240

427

128

481

583

712

884

External trigger mode

2048

1024

164

512

120

278

256

240

427

128

481

583

712

884

External trigger mode

2048

1024

193

512

120

374

256

240

702

128

481

1251

968

2052

4664

Note

• The calculation formula and the frame rate value of Start trigger mode (External

[Start trigger mode].

[Value of frame rate (unit: frame/s)]

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

(Edge trigger)

(Level trigger)

1024 / 1536 /

2048

512 ―

(Synchronous trigger readout)

trigger mode) are same as the free running mode. About this mode, refer to 10-2-5-4

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Horizontal

Vertical

Frame rate

(frame/s)

Free running mode

1/(Vn/2×1H)

2048

1024

512

120

256

240

128

481

962

7696

External trigger mode

1/(Vn/2×1H+Exp1+1H×9)

2048

1024

512

256

133

128

185

229

290

External trigger mode

1/(Vn/2×1H+1H×18)

2048

1024

512

112

256

210

128

375

615

1399

External trigger mode

1/(Vn/2×1H+1H×5)

2048

1024

512

117

256

231

128

446

832

3420

10-2-2-2 At Slow Scan

(1) Camera Link, USB 3.0

Vn = Number of vertical line (The center of the set area is the middle of the sensor .) Exp1 = 3.021 ms to 10 s 1H = 32.4812 × 10

• The Exp1 value must be input to the calculation formula below in units of seconds.

[Calculation formula, Value of Frame rate ]

-6

Calculation formula

(Edge trigger / Level trigger)

(Synchronous trigger readout)

(Fast Synchronous trigger readout) (Camera Link)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

9.744 36 µs × Exp2

32.4812 µs × Exp2

Standard scan

Slow scan

Free running mode

1 ms to 10 s

3 ms to 10 s

Free running mode (at Sub-array)

38.96 µs* to 10 s

129.99 µs to 10 s

External trigger mode

1 ms to 10 s

3 ms to 10 s

Note

* 38.96 µs (Standard sc an) and 129.99 µs (Slow scan) is the minimum exposure time

10-2-3 CONFIGURING EXPOSURE TIME

The exposure time setting can be done by absolute value. The actual exposure time setting is defined by the following formula, and the camera automatically calculates a larger and closest value from the specified exposure time sett i ng.

• The Exp1 values must be input to the calculation formula below in units of seconds.

(1) Standard scan

Exp1 = 1 ms to 10 s (at sub-array 38.96 µs to 10 s) Exp2 = Exp1 × 10

(2) Slow scan

Exp1 = 3 ms to 10 s (at sub-array 129.99 µs to 10 s) Exp2 = Exp1 × 10

÷ 9.74436 µs (round up at decimal point)

÷ 32.4812 µs (round up at decimal point)

Available setting range of the exposure time is t he following.

when sub-array is set to 8 lines vertically symmetric (4 lines in top half and 4 lines in bottom half) with respect to the horizontally center axis. The minimum exposure time vary depend on vertical line number of sub-array setting.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

10-2-4 FREE RUNNING MODE

The camera has the free running mode which the exposure and read out timing can be set by software command and controlled by an internal microprocessor. T he free run ning m od e has normal readout mode (in whic h the exposure time is longer than the 1 frame readout time) and elect rical shutter mode (in which the exposure time is shorter than the 1 frame readout time). These readout modes are automatically switched depending on the exposure time setting.

• Please contact to Hamamatsu subsidiary or local distributor for the detail of the timing information.

10-2-4-1 Normal readout mode

The normal readout mode is suitable for observation, monitoring, field of view and focus adjustment, and animation because it can operate with full resolution, which is faster than the video rate*. (* at Camera Link = 100 frame/s, at USB 3.0 = 30 frame/s) In addition, the exposure time can be extended to collect more signals and increase the signal-to-noise ratio if the object is dark. In the normal readout mode, the exposure time is the same or longer than the 1 frame readout time. In this mode, the frame rate depends on the exposure time, and it becomes frame rate = 1/exposure time. The maximum exposure time is 10 s.

Figure 10-4

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

10-2-4-2 Electrical shutter mode

The electrical shutter mode is used to get a proper signal level when signal overflow happens due to too much input photons in normal readout mode. In this mode, the fastest frame rate is 100 Hz (via Camer a Link) or 30 Hz (via USB 3.0) at full resoluti on even when the exposure time is short.

[Camera Link]

[USB 3.0]

Figure 10-5

Figure 10-6

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

10-2-5 EXTERNAL TRIGGER MODE

The camera has various external trigger functions to synchronize the camera with the external equipment. In the external trigger mode, the external equipment becomes a master and the camera becomes a slave.

• Please contact to Hamamatsu subsidiary or local distributor for the detail of the timing information.

10-2-5-1 Edge trigger mode

The edge trigger mode is used so that the exposure starts according to an external signal. Exposure time is set by software command. In this mode, the exposure of the first line begins on the edge (rising/falling) timing of the input trigger signal into the camera. (1023H and 1024H in the following figure) The exposure of the second line is begun after the readout time of one line passes (1022H and 1025H in the following figure), and the exposure is begun one by one for each line.

Figure 10-7 (Ex. rising edge)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

10-2-5-2 Level trigger mode

The level trigger mode is used to control both exposure start timing and exposure time length by inputting external trigger pulses. In the mode, the cam era st art s exposure at the start of high or low period of the input trigger pulse and stops exposure at the end of high or low period of the input trigger pulse. The example below is for the trigger level High. The exposure of the f irst line begins when the trigger signal becomes High, and the exposure of the second line begins after the readout time of line one passes. Each exposure begins one by one for each line. The exposure of the first line is finished when the trigger signal becomes low, and signal readout is begun. The exposure time of each line is defined by the time that the input trigger is high. The minimum trigger pulse width is 1 ms + 50 µs.

Figure 10-8 (Ex. rising edge)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

10-2-5-3 Synchronous readout trigger mode

The synchronous readout trigger mode is used for continuous imaging when it is necessary to control the exposure start timing of each frame f rom an ext ernal so urce. It is usef ul f or confoc al microscopy. For example, when the camera is used with a spinning disk confocal microscope and the camera exposure time is synchronized to the spinning disk's rotation speed, it is possible to eliminate uneven illumination (called banding noise) caused by variation of the spinning disk rotation speed. Also, it is useful for securing as long exposure time as possible while controlling the exposure start t i m i ngs by external trigger signals. It is possible to work with shorter interval time if you set f ast synchronous readout trigger mode. Fast synchronous readout trigger mode works with only Camera Link interface operation.

(1) Normal operation (when the pulse count is set as 1.)

The synchronous readout trigger mode is used for continuous imaging when it is necessary to control the exposure start timing of each frame from an outside source and also when it is necessary to secure as long exposure time as possible. In the synchronous readout trigger mode, the camera ends eac h exposur e, st arts the readout and also, at the same time, starts the next exposure at the edge of the input trigger signal (rising / falling edge). That is, the interval between the same edges of the input trigger becomes the exposure time.

Figure 10-9 (Ex. rising edge)

(2) Pulse count

Also in the synchronous readout trigger mode, synchronous readout can be controlled by specifying, set by command, the number of timing puls es to det ermine the ex posure time. The following figure shows the exposure timing when the pulse count is set as 3.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Figure 10-10 (Pulse count)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

10-2-5-4 Start trigger mode

The start trigger mode is to start operating the camera by a trigger input for a continuous imaging. It is useful to secure the frame rate as fast as possible when continuous image acquisition and not to sacrifice the exposure time. For example, when it is necessary to measure the phenomenon after stimulation, it is possible to start continuous image acquisition at the stimulation timing.

The start trigger mode is to start operating the camera by a trigger input for continuous imaging, and it works at the highest frame rate because it is operated in inter nal trigger m ode. In the st art trigger mode, the camera starts exposure and switches to internal trigger mode by the edge of an external trigger signal (rising / falling edge).

Figure 10-11 (Ex. rising edge)

10-2-5-5 External trigger delay function

In most cases when a delay is ne eded between th e laser pulse em ission and th e exposure st art is needed, a delay unit is set between the laser and camera to control trigger timing. In each external trigger mode of the camera, the delay can be set to the trigger signal input to the camera by command. With this setting, a range of trigger can be arranged without a delay unit. The range for delay time is 0 µs to 10 s (10 µs steps).

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

Reference

signal

Camera outputs a pulse after certain delay, from the end of sensor readout. Also the pulse width can be set.

Camera outputs a pulse after certain delay, from the beginning of readout. Also the pulse width can be set.

10-2-6 TRIGGER OUTPUT

The camera provides a ra nge of trigge r output sig nals t o synchr onize with an external instrument and the camera becomes the master and the external instrument becomes the slave. There are three different trigger output functions as follows. Also, it can output continuous High output (High output fixed) or continuous Low output (Low output fixed).

These three different trigger output functions can be sel ected by software command, and they are output from Timing out connector.

• Please refer to Figure 10-4 to Figure 10-11 about details of each trigger output functions.

10-2-6-1 Global exposure timing output

It shows the global exposure timing where all lines expose at the same time. There is a case that one event is divided into two frames because the timing of the exposure in each line is different for the rolling shutter. However, by using the Global exposure timing output the global exposure becomes possible for the phenomenon that happens for t his peri od. Global expo sur e timing output shows the period where all lines expose at the same time.

• There is no output signal when the exposure time is less than the frame rate.

10-2-6-2 Programmable timing output

By using the programmable timing output, synchronizing external devices is simple. A system that needs simple timing signal does not require a delay unit or pulse generator. It is possible to program and output a pulse that has an optional pulse width and an optional delay time to the end of readout timing or Vsync. The setting range for delay time is 0 µs to 10 s, and the setting range for pulse width is 10 µs to 10 s.

The relation between the param eter which can be set with each ref erence signal , and an out put signal becomes below .

Output signal

Read End

Vsync

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Figure 10-12 Programmable timing output

10-2-6-3 Trigger ready output

The trigger ready output is useful to make the frame intervals as short as possible in external trigger mode. For example, when the camera is working in the edge trigger mode, the next frame can start after the previous frame exposure is done. Thus, the camera cannot accept a trigger for the next frame during the exposure period. To reduce useless time to be as short as possible, it is necessary to know the period when the camera can accept a trigger for the next frame. The trigger ready output shows the trigge r ready period w hen the came ra can acce pt an external trigger in the external trigger mode.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Note

• Please refer to Figure 10-2-2 FRAME RATE CALCULATION about the frame rate value and

10-2-7 SLOW SCAN

It can change from the standard scan (Default) to the slow scan with a command. Read-out noise is reduced with slow scan.

calculation formula with slow scan.

10-2-7-1 Timing Diagram

The read-out time is 33 ms at full frame and the frame rate is 30 frames/s at fastest. The timing diagram for slow scan is similar to the timing diagram for the standard scan except readout time. Please refer to the timing diagram of standard scan for each trigger mode.

Figure 10-13

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

10-2-8 GLOBAL RESET

10-2-8-1 Timing diagram

Global reset function enables to reset the electric charge of all pixels at the same time. Then all pixels can start exposure at the same time. Global reset can work with Edge trigger mode and Level trigger mode.

[Edge trigger mode]

Figure 10-14

[Level trigger mode]

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Figure 10-15

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

10-3 LIGHT SHEET MODE

10-3-1 READ-OUT DIRECTION

With normal area mode, the camera readout from the center line to the top line and to the bottom line simultaneously. With light sheet mode, the camera readout from the top to the bottom line or from the bottom to the top line. Light sheet mode works with only Camera Link interface operation. And the fastest frame rate is 49 frames /s.

Figure 10-16 Normal area mode Figure 10-17 Light sheet mode

(1) Top to bottom read-out：The data is read-out from the top to the bottom line. (2) Bottom to top read-out：The data is read-out from the bottom to the top line.

Figure 10-18 Top to bottom read-out Figure 10-19 Bot tom to top read-out

Light sheet mode supports three operation modes. Also light sheet mode supports sub-array read-out.

(1) Free running mode (2) Edge trigger mode (3) Start trigger mode

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

1/(Exp1+(Vn+10)×1H)

Horizontal width × Vertical width

Frame rate (frame/s)

2048

1024

512

196

256

385

128

743

1386

5701

7330

Settings

Size

Position

Readout time = Vn × 1H

10-3-2 FRAME RATE CALCULATION

[Calculation formula]

Vn = Number of vertical line (the number of lin es from the middle of the sensor.) Exp1 = 9.7 µs to 10 s

1H = HLN / (266 × 10

• The Exp1 value must be input to the calculation formula below in units of seconds.

[Value of frame rate]

) (HLN = 2592 to 26600000)

Size and position for sub-array read-out can be configured according to the table below.

Vertical 4 lines steps

10-3-3 READ-OUT TIME OF THE HORIZONTAL LINE

Read-out time and exposure time can be varied with light sheet mode.

The range of exposure time with light sheet mode is as shown below. The maximum exposure time can be decided according to the readout time of a frame, which is longer than 10 s or not.

1H to 1H × Vn (1H × Vn < 10 s) 1H to 10 s (1H × Vn ≧10 s)

Vn = Number of vertical line 1H = HLN / (266 × 10 (HLN = 2592 to 26 600 000)

): 9.7436 µs to 100 ms

10-3-4 TIMING DIAGRAM

[Free running mode]

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

[Edge trigger mode]

Figure 10-20 Free running mode

Figure 10-21 (Ex. rising edge)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

[Start trigger mode]

Figure 10-22 (Ex. rising edge)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Camera outputs a pulse after certain delay from the end of sensor readout. Also the pulse width can be set.

Camera outputs a pulse after certain delay from the beginning of readout. Also the pulse width can be set.

Camera outputs a pulse after certain delay from the ending of readout. Also the pulse width can be set.

10-3-5 PROGRAMMABLE TIMING OUTPUT

By using the programmable timing output, synchronizing with external devices is simple. A system which needs simple timing signal does not require a delay unit or pulse generator. It is possible to program and output a pulse that has an optional pulse width and an optional delay time to the end of readout timing, Vsync or Hsync. The range of delay is 0 µs to 10 s, and the range of pulse width is 10 µs to 10 s.

The relation between the parameter which can be set with each reference signal, and an output signal becomes as shown below.

Reference signal Output signal

Read End

Vsync

Hsync

Figure 10-23 Programmable timing output

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

0H 1H

・・

1023H 1024H . . 2046H 2047H

Hsync

Expos ure

External trigger

Delay

Puls e Width

Figure 10-24 Programmable timing output referenced with Hsync

When you choose Hsync for the reference of programmable timing output, camera can output some pulses before start the exposure. It is called as Pre-Hsync. You can set the number of Pre-Hsync with a software command.

10-4 REAL-TIME CORRECTI ON FUNCTIONS

There are a few pixels in FL-400 that have slightly higher readout noise performance compared to surrounding pixels. The camera has real-time variant pixel correcti on features to improve image quality. The correction is performed in real-time without sacrificing the readout speed at all. This function can be turned O N and O F F . (Default is ON)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

• Be careful not to input too strong light such as high-energy las er into FL-400 because FL-400 may be damaged by over light.

11. P RE CAUTION WHEN USING FL-400

This camera uses FL-400 (scientific CMOS image sensor). Careful attention must be paid to the following points when using FL-400:

(1) White spot

Subjecting FL-400 to exten ded exp osures may cause f ailure i n p art of the sili con wafe r, resulting in white spots. Currently this phenomenon is not currently preventable. If FL-400 is at a fixed temperature, recurrence of the white spot increases proportionally with the exposure time, so this can be rectified with dark subtraction*. Cosmic ray may generate white spot.

* After acquiring an image using a certain exposure time is loaded, the FL-400 is exposed to darkness for the same

amount of time, and another image is obtained. After this, the difference between the images is determined, and the data for the dark portion of the original image is nullified.

(2) Folding distortion

A rough-edged flicker may be visible when imaging striped patterns, lines, and similar subject matter.

(3) Over light

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

• Please use a plastic tweezers and take extra care not to scratch the glass window with the

12. MAINTENANCE

12-1 CARE

Perform cleaning of this equipment with the dry soft cloth.

• Do not wipe with a damp cloth or unclean cloth.

Then, the glass window on the image sensor should be c leaned according to the following.

(1) Blow the dust from the glass window with an air duster. (2) Moisten a lens cleaning paper with a little ethanol, and wipe over center area of the

window, gently.

• Use Lens Cleaning Paper for cleaning of glass window in front of the image sensor.

tweezers. Even with plastic tweezers, there is possibility to make scratch on the glass window in case tweezers touch it.

• Please avoid touching the surrounding parts of image area when wiping the glass window.

(3) Confirm whether dust is not left.

Attach the camera to an optics, and check if there is dust or not under the uniform light condition. If there is dust on the image, please clean the glass window again.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

please conduct a process to soften water before use it.

Note

Pure water

Note

clean cooling water path.

Note

Soft water from tap

• Clean cooling water path every 6 months.

Note

Bottled water

manufacturer.

12-2 INFORMATION ON CO OLING WATER

FOR THE CIRCULATING WATER COOLER

• Regarding handling cooling water and circulating water cooler, please refer to instruction

manual attached to the circulating water cooler.

• It is recommended to use soft water (except deionized water) for cooling water.

• Do not use hard water for cooling. It cause inside of cooling water circulating path to be

12-2-1 WHEN USING COOLING WATER OTHER THAN RECOMMENDED

calcified or corroded and it result lower flow rate or water flow stop. When using hard water,

• Deionized water is not appropriate for cooling water. There is possibility that deionized water absorb component of cooling water path and it may cause corrosion. In ad dition deionized water is easy to be polluted and cause impurity, sliminess or forming foreign subs tances. It cause lower flow rate or water flow stop.

Distilled water / Deionized water

• When using the camera inside clean room, it is possi ble to use distilled water or deionized

water by conducting periodical check. However please notice it increases possibility of corrosion inside cooling water path, lowering flow rate or water flow stop.

• Monthly check : Check water impurity, non-existence of sliminess, foreign particle is

not mixed with water or not adhered inside water path and no unusual odor. If you find any of the issues, please exchange cooling water and

• It is possible to use soft water from tap with conducting periodical change of cooling water and check up. However please notice it increases possibility of corrosion inside cooling water path, lowering flow rate or water flow stop.

• Monthly check : Check water impurity, non-existence of sliminess, foreign particle is

not mixed with water or not adhered inside water path and no unusual odor. If you find any of the issues, please exchange cooling water and clean cooling water path.

• Exchange cooling water every 3 months.

• One example of soft water which is commonly available is mineral water (Hardnes s less

than 70). Please check hardness of water by referring product information of bottled water

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Cause

Measures

Chapter

AC adaptor or other cable is loose

Reconnect the cable

AC adaptor or other cable is broken

Replace the cable

The correct command has not been sent to the camera

Cause

Measures

Chapter

Cause

Measures

Chapter

Lens is not focused

Contact Hamamatsu subsidiary or local distributor

Condensation appear

Confirm the operating environmental conditions

Cause

Measures

Chapter

Lens mount cap has been left on

Remove the cap

Amount of light is too much or too low

Reduce amount of light

Cause

Measures

Chapter

Too much amount of light

Reduce amount of light

Contrast enhancement is too high

Reduce gain

Cause

Measures

Chapter

Exogenous noise

Find and remove cause

Poor connection of internal connect or

local distributor

Defective circuit system

13. TROUBLESHOOTING CHECKLIST

If an abnormality occurs, look up the possible causes in the following tables and, if necessary, report the details to Hamamats u subsidiary or local distributor.

13-1 IMAGE IS NOT TRANSFERRED

Recheck command

13-2 ALTHOUGH IMAGES ARE TRANSFFERED

(1) Scratches or discoloration visible on the screen

Lens is dirty Wipe the lens 12

(2) Image is blurred

(3) Only shadowed images are output

(4) All screens overflow

(5) Noise appears on the screen

Contact Hamamatsu subsidiary or

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Camera Link

USB 3.0

Imaging device

Scientific CMOS image sensor FL-400

Effective number of pixels

2048 (H) × 2048 (V)

Cell size

6.5 µm (H) × 6.5 µm (V)

Effective area

13.312 mm (H) × 13.312 mm (V)

Full well capacity (typ.)

30 000 electrons

Dynamic range *1

37 000 : 1

Cooling method

Peltier device + Forced air-cooled, Water-cooled

at Forced air-cooled

- 10 °C (Ambient temperature: + 20 ºC)

at Water-cooled

- 20 °C (Water temperature: + 20 ºC)

- 30 °C (typ.)

Water temp. 15 °C, Ambient temp. 20 °C)

100 frame/s 30 frame/s

at 1024 lines at center position

200 frame/s 60 frame/s

at 8 lines at center position

25 655 frame/s 7 696 frame/s

at Horizontal 512 pixels at center position

Normal readout mode

1×1

Binning readout mode

2×2,4×4 (Digital binning) *2

Configurable for each

horizontal 512 pixels.

Readout time

20 ms to 204.8 s

Exposure time

9.7 µs to 10 s

Readout mode

Full/Sub-array

at - 10 °C

0.06 electron/pixel/s

at - 20 °C

0.02 electron/pixel/s

at - 30 °C

0.006 electron/pixel/s

A/D bit

16 bit

Linearity

Less than 3 %

Free running mode

1 ms to 10 s

Free running mode / Sub-array mode

External trigger mode

1 ms to 10 s

14. SPECIFICATIONS

14-1 CAMERA SPECIFICATIONS

(1) Electric specifications

Readout noise (typ.)

Cooling temperature

Frame rate

Top: standard scan Bottom: slow scan

Readout mode

Light sheet mode

at Maximum cooling

at Full resolution

at 8 lines

Sub-array readout mode

1.0 electrons (median) 1.6 electrons (r.m.s) at standard scan

0.8 electrons (median) 1.4 electrons (r.m.s) at slow scan

(at Water-cooled;

30 frame/s

60 frame/s

7894 frame/s

－ 25 655 frame/s

Upper and lower

symmetric area

vertical 8 pixels and

― ― ―

Dark current

Exposure time (Standard scan)

38.96 µs to 10 s

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Camera Link

USB 3.0

Free running mode

3 ms to 10 s

Free running mode / Sub-array mode

External trigger mode

3 ms to 10 s

Conversion factor

0.46 electrons/count (typ.)

Dark offset

100 counts (at Normal readout)

Edge trigger,

Start trigger

External input

(Camera Link)

External trigger input level

3.3 V LVCMOS level

External trigger output level

3.3 V LVCMOS level

External trigger delay function

0 µs to 10 s (10 µs steps)

Global exposure timing output,

(Continuous High or Low output)

Real-time offset correction,

Real-time defect pixel correction (Default)

Camera Link Full

configuration*3

Lens mount

C-mount (C11440-22CU) / F-mount (C11440-22CU01)

Input power supply

DC 12 V

Typical output

Power consumption

55 W

Input power supply

AC 100 V to AC 240 V 50 Hz / 60 Hz 2.5 A

Typical output

DC 12 V 8.34 A

Power consumption

70 VA

Note

• Fluctuations of input power supply voltages are not to exceed ± 10 % of

Exposure time (Slow scan)

External trigger Function

External signal input

External signal output

Image processing function

Interface

129.99 µs to 10 s

Level trigger,

Synchronous readout trigger,

(SMA connector)

Trigger ready output, Programmable timing output 1, Programmable timing output 2,

Programmable timing output 3

Real-time gain correction,

External input

(SMA connector)

USB 3.0 Super Speed

* 1 Calculated from the ratio of the full well capacity and the readout noise. * 2 Digital binning processing in the camera. * 3 Original mode based on 80 bit mode

(2) Power supply specifications

Camera

AC adaptor

the nominal voltage.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Ambient operating temperature

0 ºC to + 40 ºC

Ambient storage temperature

-10 ºC to + 50 ºC

Ambient operating humidity

at Forced air-cooled

Less than 70 %, no condensation

Less than 50 % (at ambient temperature 20 °C)

Less than 40 % (at ambient temperature 20 °C)

Ambient storage humidity

Less than 90 %, no condensation

Operating space

Indoor, altitude up to 2000 m

Note

(3) Operating environment

at Water-cooled

at Maximum cooling

(4) Dimensional outline and weight

Dimensional outline 85 mm (W) × 85.5 mm (H) × 125 mm (D) Weight Approx. 2.0 kg (Camera only)

• Please see Chapter 15 [DIMENSIONAL OUTLINES] for detail of dimensions.

• Be careful not to drop off the camera or not drop underfoot whe n making it

move because it is approx. 2.0 kg.

(5) Applicable standards

EMC EN61326-1: 2006 Class A

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

14-2 CONDENSATION

At the Water-cooling, if ambient temperature and ambient humidity become high, condensation will take place easily. Use the camera under the environment where condensation will not take place referring to the following graph.

Figure 14-1

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

400 500

600

700 800 900 1000

Wavelength [nm]

Q.E. [%]

14-3 SPECTRAL RESPONSE CHARACTERISTICS

Figure 14-2

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Camera connector

Frame grabber connector

Channel Link signal

Inner Shield

X0-

X1-

X2-

Xclk-

X3-

SerTC+

SerTFG-

CC1-

CC2+

CC3-

CC4+

Inner Shield

X0+

X1+

X2+

18 9 Xclk+

19 8 X3+

SerTC-

SerTFG+

22 5 CC1+

23 4 CC2-

24 3 CC3+

25 2 CC4-

Inner Shield

14-4 INTERFACE SPECIFIC ATIONS

14-4-1 CAMERA LINK INTERFACE

The camera is based on Camera Link interface [Camera Link full configuration Deca mode], that transfers 80 bit data in parallel (8 bit x 10 port). It is an extended interf ace of Camera Link full configuration Standard.

(1) Pin assignments of Camera Link connector 1 (SDR-26)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Camera connector

Frame grabber connector

Channel Link signal

Inner Shield

Y0-

Y1-

Y2-

Yclk-

Y3-

Terminated

Z0-

Z1-

Z2-

Zclk-

Z3-

Inner Shield

Y0+

Y1+

Y2+

18 9 Yclk+

19 8 Y3+

Terminated

21 6 Z0+

22 5 Z1+

23 4 Z2+

24 3 Zclk+

25 2 Z3+

Inner Shield

(2) Pin assignments of Camera Link connector 2 (SDR-26)

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

28 bit solution

pin name

Plug No.1,

Channel Link X

Plug No.2,

Channel Link Y

Plug No.3,

Channel Link Z

TxIN0

Port A0

D0_0

Port D2

D1_10

Port G5

D3_5

TxIN1

Port A1

D0_1

Port D3

D1_11

Port G6

D3_6

TxIN2

Port A2

D0_2

Port D4

D1_12

Port G7

D3_7

TxIN3

Port A3

D0_3

Port D5

D1_13

Port H0

D3_8

TxIN4

Port A4

D0_4

Port D6

D1_14

Port H1

D3_9

TxIN5

Port A5

D0_5

Port D7

D1_15 (MSB)

Port H2

D3_10

TxIN6

Port A6

D0_6

Port E0

D2_0

Port H3

D3_11

TxIN7

Port A7

D0_7

Port E1

D2_1

Port H4

D3_12

TxIN8

Port B0

D0_8

Port E2

D2_2

Port H5

D3_13

TxIN9

Port B1

D0_9

Port E3

D2_3

Port H6

D3_14

TxIN10

Port B2

D0_10

Port E4

D2_4

Port H7

D3_15 (MSB)

TxIN11

Port B3

D0_11

Port E5

D2_5

Port I0

D4_0

TxIN12

Port B4

D0_12

Port E6

D2_6

Port I1

D4_1

TxIN13

Port B5

D0_13

Port E7

D2_7

Port I2

D4_2

TxIN14

Port B6

D0_14

Port F0

D2_8

Port I3

D4_3

TxIN15

Port B7

D0_15 (MSB)

Port F1

D2_9

Port I4

D4_4

TxIN16

Port C0

D1_0

Port F2

D2_10

Port I5

D4_5

TxIN17

Port C1

D1_1

Port F3

D2_11

Port I6

D4_6

TxIN18

Port C2

D1_2

Port F4

D2_12

Port I7

D4_7

TxIN19

Port C3

D1_3

Port F5

D2_13

Port J0

D4_8

TxIN20

Port C4

D1_4

Port F6

D2_14

Port J1

D4_9

TxIN21

Port C5

D1_5

Port F7

D2_15 (MSB)

Port J2

D4_10

TxIN22

Port C6

D1_6

Port G0

D3_0

Port J3

D4_11

TxIN23

Port C7

D1_7

Port G1

D3_1

Port J4

D4_12

TxIN24

LVAL

Port G2

D3_2

Port J5

D4_13

TxIN25

FVAL

Port G3

D3_3

Port J6

D4_14

TxIN26

Port D0

D1_8

Port G4

D3_4

Port J7

D4_15 (MSB)

TxIN27

Port D1

D1_9

LVAL

Pixel Clock

A,B,C

Pixel Clock

G,H,I,J

This signal show the period during which the line part of the image data from This is “ON” when during the period the line is active.

FVAL

(Frame Valid signal)

This signal shows the period during which the vertical part of the image data from FL-400 is in effect. This is “ON” during the period the frame is active.

This is the image signal data from FL-400 converted A/D. Please see 14-4-2 [OUTPUT TIMING SPECIFICATIONS] for details.

(3) Camera Link bit assignments

Port

TxCLKIn PClk

LVAL

(Line Valid signal)

D0_0 to D4_15

(Digital image data)

FL-400 is in effect.

”D0 to D4” has 16 bit data in each. MSB shows the most significant bit.

PClk Pixel Clock D,E,F PClk

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

CL Port F Data

Tx Clock

C.L Fvalid

CL Port A Data

CL Port E Data

CL Port D Data

CL Port C Data

CL Port B Data

C.L Lvalid

CL Port I Data

CL Port J Data

CL Port H Data

CL Port G Data

C.L Lvalid

CL Fvalid

CL Lvalid

85 MHz

V1023

V1024

V1022

V2045V1V2046F1V2047

F2F1F3

V1023

V1024

410 cloc k

4 cloc k

H0 L

H5 L

H10 L

H15 L

H20 L

H2035 L

H2040 L

H2045 L

H0 L

H5 L

H10 L

H15 L

H20 L

H0 U

H5 U

H10 U

H15 U

H20 U

H2035 U

H2040 U

H2045 U

H0 U

H5 U

H10 U

H15 U

H20 U

L: Lower byte

U: Upper byte

H1 L

H6 L

H11 L

H16 L

H21 L

H2036 L

H2041 L

H2046 L

H1 L

H6 L

H11 L

H16 L

H21 L

H1 U

H6 U

H11 U

H16 U

H21 U

H2036 U

H2041 U

H2046 U

H1 U

H6 U

H11 U

H16 U

H21 U

H2 L

H7 L

H12 L

H17 L

H22 L

H2037 L

H2042 L

H2047 L

H2 L

H7 L

H12 L

H17 L

H22 L

H2 U

H7 U

H12 U

H17 U

H22 U

H2037 U

H2042 U

H2047 U

H2 U

H7 U

H12 U

H17 U

H22 U

H3 L

H8 L

H13 L

H18 L

H23 L

H2038 L

H2043 L

0xFF

H3 L

H8 L

H13 L

H18 L

H23 L

H3 U

H8 U

H13 U

H18 U

H23 U

H2038 U

H2043 U

0xFF

H3 U

H8 U

H13 U

H18 U

H23 U

H4 L

H9 L

H14 L

H19 L

H24 L

H2039 L

H2044 L

Line Info

H4 L

H9 L

H14 L

H19 L

H24 L

H4 U

H9 U

H14 U

H19 U

H24 U

H2039 U

H2044 U

Line Info

H4 U

H9 U

H14 U

H19 U

H24 U

Camera Link Timing (Normal)

14-4-2 OUTPUT TIMING SPECIFICATIONS

(1) Normal readout

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

CL Port F Data

CL Port G Data

CL Port H Data

CL Port I Data

CL Port C Data

CL Port D Data

85 MHz

CL Port E Data

CL Port J Data

C.L Lvalid

C.L Fvalid

CL Fvalid

CL Lvalid

Tx Clock

C.L Lvalid

C.L Fvalid

CL Port A Data

CL Port B Data

V511

V512

V510

V1021

V1022

V1023

F2F1F3

V511

V512

205 cloc k

209 cloc k

H0 L

H5 L

H10 L

H15 L

H20 L

H1010 L

H1015 L

H1020 L

H0 L

H5 L

H10 L

H15 L

H20 L

H0 U

H5 U

H10 U

H15 U

H20 U

H1010 U

H1015 U

H1020 U

H0 U

H5 U

H10 U

H15 U

H20 U

L: Lower byte

U: Upper byte

H1 L

H6 L

H11 L

H16 L

H21 L

H1011 L

H1016 L

H1021 L

H1 L

H6 L

H11 L

H16 L

H21 L

H1 U

H6 U

H11 U

H16 U

H21 U

H1011 U

H1016 U

H1021 U

H1 U

H6 U

H11 U

H16 U

H21 U

H2 L

H7 L

H12 L

H17 L

H22 L

H1012 L

H1017 L

H2 L

H7 L

H12 L

H17 L

H22 L

H2 U

H7 U

H12 U

H17 U

H22 U

H1012U

H1017 U

H2 U

H7 U

H12 U

H17 U

H22 U

H3 L

H8 L

H13 L

H18 L

H23 L

H1013 L

H1018 L

H3 L

H8 L

H13 L

H18 L

H23 L

H3 U

H8 U

H13 U

H18 U

H23 U

H1013 U

H1018 U

H3 U

H8 U

H13 U

H18 U

H23 U

H4 L

H9 L

H14 L

H19 L

H24 L

H1014 L

H1019 L

Line Info

H4 L

H9 L

H14 L

H19 L

H24 L

H4 U

H9 U

H14 U

H19 U

H24 U

H1014 U

H1019 U

Line Info

H4 U

H9 U

H14 U

H19 U

H24 U

H1022 L

H1022 U

H1023 L

H1023 U

Camera Link Timing (2x2 Binning)

(2) 2x2 binning readout

CL Port D Data

CL Port E Data

CL Port F Data

CL Lvalid

CL Port A Data

CL Port B Data

CL Port C Data

CL Port G Data

CL Port H Data

CL Port I Data

CL Port J Data

C.L Fvalid

C.L Lvalid

C.L Fvalid

CL Fvalid

Tx Clock

C.L Lvalid

85 MHz

0xFF

V255

V256

V254

V510

V511

F1F3F4

V255

V256

103 cloc k

311 cloc k

H0 L

H5 L

H10 L

H15 L

H20 L

H500 L

H505 L

H510 L

H0 L

H5 L

H10 L

H15 L

H20 L

H0 U

H5 U

H10 U

H15 U

H20 U

H500 U

H505 U

H510 U

H0 U

H5 U

H10 U

H15 U

H20 U

L: Lower byte

U: Upper byte

H1 L

H6 L

H11 L

H16 L

H21 L

H501 L

H506 L

H511 L

H1 L

H6 L

H11 L

H16 L

H21 L

H1 U

H6 U

H11 U

H16 U

H21 U

H501 U

H506 U

H511 U

H1 U

H6 U

H11 U

H16 U

H21 U

H2 L

H7 L

H12 L

H17 L

H22 L

H502 L

H507 L

H2 L

H7 L

H12 L

H17 L

H22 L

H2 U

H7 U

H12 U

H17 U

H22 U

H502U

H507 U

H2 U

H7 U

H12 U

H17 U

H22 U

H3 L

H8 L

H13 L

H18 L

H23 L

H503 L

H508 L

H3 L

H8 L

H13 L

H18 L

H23 L

H3 U

H8 U

H13 U

H18 U

H23 U

H503 U

H508 U

H3 U

H8 U

H13 U

H18 U

H23 U

H4 L

H9 L

H14 L

H19 L

H24 L

H504 L

H509 L

Line Info

H4 L

H9 L

H14 L

H19 L

H24 L

H4 U

H9 U

H14 U

H19 U

H24 U

H504 U

H509 U

Line Info

H4 U

H9 U

H14 U

H19 U

H24 U

Camera Link Timing (4x4 Binning)

V509

(3) 4x4 binning readout

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

CL Port F Data

CL Port G Data

CL Port D Data

85 MHz

CL Port E Data

CL Port B Data

CL Port C Data

CL Port J Data

C.L Lvalid

C.L Fvalid

CL Port H Data

CL Port I Data

Tx Clock

CL Port A Data

C.L Lvalid

C.L Fvalid

CL Lvalid

CL Fvalid

V(o1+w)

F2F1F3

V offset1 + width (Top region)

410 cloc k

4 cloc k

H0 L

H5 L

H10 L

H15 L

H20 L

H2035 L

H2040 L

H2045 L

H0 L

H5 L

H10 L

H15 L

H20 L

H0 U

H5 U

H10 U

H15 U

H20 U

H2035 U

H2040 U

H2045 U

H0 U

H5 U

H10 U

H15 U

H20 U

L: Lower byte

U: Upper byte

H1 L

H6 L

H11 L

H16 L

H21 L

H2036 L

H2041 L

H2046 L

H1 L

H6 L

H11 L

H16 L

H21 L

H1 U

H6 U

H11 U

H16 U

H21 U

H2036 U

H2041 U

H2046 U

H1 U

H6 U

H11 U

H16 U

H21 U

H2 L

H7 L

H12 L

H17 L

H22 L

H2037 L

H2042 L

H2047 L

H2 L

H7 L

H12 L

H17 L

H22 L

H2 U

H7 U

H12 U

H17 U

H22 U

H2037 U

H2042 U

H2047 U

H2 U

H7 U

H12 U

H17 U

H22 U

H3 L

H8 L

H13 L

H18 L

H23 L

H2038 L

H2043 L

0xFF

H3 L

H8 L

H13 L

H18 L

H23 L

H3 U

H8 U

H13 U

H18 U

H23 U

H2038 U

H2043 U

0xFF

H3 U

H8 U

H13 U

H18 U

H23 U

H4 L

H9 L

H14 L

H19 L

H24 L

H2039 L

H2044 L

Line Info

H4 L

H9 L

H14 L

H19 L

H24 L

H4 U

H9 U

H14 U

H19 U

H24 U

H2039 U

H2044 U

Line Info

H4 U

H9 U

H14 U

H19 U

H24 U

Camera Link Timing (Vertical Sub Array 2 same size regions readout)

V offset2 (Bottom region )

V(o2)

V(o1+w-1)

V(o2+w-2)

V(o1-1)

V(o2 + w- 1)

V(o1)

V(o2+w)

V(o1-2)

(4) Sub-array readout

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

(Unit: mm)

15. DI ME NSI O NAL OUTLINES

15-1 C11440-22CU

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

(Unit: mm)

15-2 C11440-22CU01

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

(1) Unless otherwise stated by Hamamatsu subsidiary or local distributor, this system

under warranty for 24 months from the delivery date.

- Degradation with cosmic rays, the radiation (X-rays, gamma ray

s, UV light, etc.) of

FL-400 is excepted.

(2) The warrant

y only covers defects in the materials and manufacturing of the system. You may be liable for repairs during the warranty period in the event of a natural disaster or if you handle the system contrary to the instructions in this manual, use it without due caution, or try to modify it.

(3) We will repair the system or replace it, subject to availability, free of charge within the

terms of the warranty.

REPAIRS

(1)

If you no tice anything wrong wi th the ca mera, confirm w hether or not i t is malfunctioning

by referring to the troubleshooting checklist in this instruction manual.

You must first

clarify the symptoms in order to avoid any misunderstanding or error.

(2) If you have any trouble or are unclear about anything, contact Hamamatsu subsidiary

or local distributor giving the product name, serial num ber and details of the problem. If Hamamatsu Photonics consider the problem to be a malfunction, we will decide whether dispatch an engineer or have the camera ret urned to us for repairs.

16. WARRANTY

Hamamatsu Photonics have fully inspected t his system and checked that its performance conforms to specifications. I n the unlikely event of breakdow n or other malfuncti on, contact Hamamatsu subsidiary or local distributor.

C11440-22CU/C11440-22CU01 Instruction manual_Ver.1.4

Manufacturer

HAMAMATSU PHOTONICS K. K., Systems Division

812 Joko-cho, Higashi-ku, Hamamatsu City, 431-3196, Japan Telephone (81) 53-431-

0124, Fax: (81) 53-435-1574

E-mail: export@sys.hpk.co.jp

Local contact information worldwide could be found under:

www.hamamatsu.com

 The contents of this manual are subject to change without notice.  The un autho rized repr oducti on or distri bution of pa rts or all of t his manua l is prohibit ed.  If one of the following problems occurs, please contact Hamamatsu Photonics.

(See the CONTACT INFORMATION.) We will deal with the problem immediately.

 Some contents of the manual are dubious, incorrect or missing.  Some pages of the manual are missing or in the wr ong order.  The manual is missing or dirty.

Hamamatsu Photonics C11440-22CU, C11440-22CU01 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

1. SAFETY PRECAUTIONS

1-1 INDICATION OF THE SYMBOLS

1-2 CLASSIFICATION OF WARNING

2. CHECK THE CONTENTS OF PACKAGE

3. INSTALLATION

4. OVERVIEW

5. FEATURES

(1) Readout noise

(2) Cooling structure

(3) Pixel number and pixel size

(4) Readout method

(5) Frame rate (readout speed)

(6) Real-time correction functions

(7) Interface

(8) Camera readout modes

(9) Camera operation modes

6. NAME AND FUNCTION OF THE PARTS

(1) C11440-22CU

(2) C11440-22CU01

① Lens mount

② WATER connector [WATE R] (at Water-cooling)

③ Camera Link interface connector [DIGITAL OUT 1]

④ Camera Link interface connector [DIGITAL OUT 2]

⑤ Trigger input connector [EXT.TRIG]

⑥ Timing out connector 1,2,3 [TIMING 1,2,3]

⑦ STATUS lamp [STATUS]

⑧ Power switch [POWER]

⑨ DC power input connector [DC IN]

⑩ USB 3.0 interface connector [USB 3.0]

⑪ Air outlet

7. CONNECTION

 Overall connection

① AC adaptor

② Cooling water hose (at Water-cooling: Option)

③ Camera Link interface cable 1 (Optio n)

④ Camera Link interface cable 2 (Optio n)

⑤ USB 3.0 interface cable (Option)

8. WATER COOLING

8-1 CAUTIONS

(1) Cooling water

(2) Recommendation ambient temperature

(3) Condensation

(4) Handling of the circulating water cooler

(5) Start water cooling and water cooping in operation

(6) Cooling water hose

(7) Connection of the cooling water hose

(8) Deterioration of the cooling water hose

8-2 CONNECTION OF WATER COOLING HOSES

(3) Set the camera onto a microscope (If the camera is used on the microscope).

(4) Connect the hose onto the circulating water cooler.

(5) Turn on the circulating water cooler and confirm the cooling water is flowing normally.

8-3 DISCONNECTION OF WATER COOLING HOSES

(3) Remove water or water drop inside the hose and the camera by air.

(4) Remove the camera fro m t he mi croscope (if the camera is used on the microscope).

(5) Place the camera on the stable table.

(6) Change the WATER connector direction to be downward.

(7) Remove hoses one by one, and wipe water.

9. OPERATION

9-1 PRECAUTIONS

(1) Cooling method

(2) Ambient temperature

(3) Protection circuit

9-2 PREPARATION FOR IMAGING

9-2-1 WHEN USING AIR-COOLING

9-2-2 WHEN USING WATER-COOLING

9-3 END OF IMAGING

10. DE S CRIPTION OF VARIOUS FUNCTIONS

10-1 THEORY OF CMOS IMAGE SENSOR

10-2 NORMAL AREA MODE

10-2-1 READOUT METHOD (SCAN MODE)

(1) Readout direction

(2) Normal readout

(3) Binning readout

(4) Sub-array readout

10-2-2 FRAME RATE CALCULATION

10-2-2-1 At Standard Scan