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PCV-3000
User Manual
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Grant PCV-3000 User Manual

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Centrifuge/Vortexer
Multi-spin
PCV-3000
Operating instructions
For version V.2GW
Multi-spin PCV-3000
Operating Instructions
Contents
1 Safety ...........................................................................................4
2 General Information....................................................................6
3 Getting started ............................................................................8
4 Operation of PCV-3000.............................................................10
5 Specifications ...........................................................................12
6 Guarantee and service .............................................................13
7 Declaration of Conformity........................................................14
Multi-spin PCV-3000 Operating Instructions
Version 2.02 - August 2012

Page 3

The following symbol means:
1. Safety
Caution: Read these operating instructions fully before use and pay particular attention to
Always observe the following safety precautions.
Caution! Care must be taken before every operating session, please check that:
If you are using more than one type of Grant Bio product, be aware that the PSU connections are common throughout the range of units. Therefore, before connecting the unit to the PSU check Voltage and current ratings for the particular unit.
GENERAL SAFETY
Operation of the unit must be carried out according to the given operating manual.
The unit should be saved from shocks or drops.
The unit must be stored and transported only in a horizontal position (see marking on the package).
After transport or storage allow the unit to dry out (2-3 hrs) before connecting to the mains.
Before using any cleaning or decontamination method except those recommended by the manufacturer, check with the manufacturer that the proposed method will not damage the equipment.
Use only original accessories (rotors, pins, etc.) provided by the manufacturer and ordered specifically for this model.
ELECTRICAL SAFETY
Connect only to the external power supply with a voltage corresponding to that on the serial number label.
Use only the external power supply unit provided with this product.
Ensure that the external power supply is easily accessible during use.
Do not plug the unit into the mains outlet without grounding, and do not use extension lead without grounding.
Before moving the unit, disconnect it from the mains outlet.
sections containing this symbol.
The Spin-plate is secured tightly; That the micro tubes are sealed correctly;
Do not use if the Spin-plate cover has any cracks or chips; Do not open the lid until Spin-plate has stopped revolving.
Multi-spin PCV-3000
Operating Instructions
To turn off the unit, disconnect the external power supply from the mains outlet.
If liquid is spilt inside the unit, disconnect it from the external power supply and have it checked by a competent person.
DURING OPERATION
Although the PCV-3000 can be programmed for unattended use, we advise that the unit should be supervised during operation.
When siting the PCV-3000 in the laboratory ensure that the unit is level and will not slide once in operation.
Do not use rotors with visible signs of corrosion, wear or mechanical damage.
Do not fill in the tubes after they are inserted in the rotor.
Do not operate the unit in environments with aggressive or explosive chemical mixtures.
For indoor use only.
Do not place a load exceeding 0.2 kg on PCV-3000.
Do not switch on the unit with the upper lid of the body removed.
Do not use outside laboratory rooms.
Do not operate the unit if it is faulty or been incorrectly installed.
Do not make modifications to the design of the unit.
BIOLOGICAL SAFETY
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt on or inside the equipment.
Multi-spin PCV-3000 Operating Instructions
Version 2.02 - August 2012

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2. General Information
Multi-spin PCV-3000 is the next step of development of sms technology: it is a fully automated device utilising the sms algorithm with good repeatability. Productivity of sms algorithm using Multi-Spin is 10 times faster (up to 30 min per 1000 samples) than that for centrifuge-vortex Combi-Spin.
Combi-Spin (the previous name Micro-Spin) was invented and published for the first time by Biol. Dr. V.Bankovskis in 1989 and after the successful approbation and finishing patented in 1994 (V.K.Bankovskis et al., Riga, Latvia, Pat. No. P94-74).
Spin-mix-spin technology automates the repeated steps of spinning down of the sample followed by vortex mixing. This cycle of repeats significantly reduces the likelihood of contamination and therefore errors in PCR analysis as well as the overall time taken for sample preparation.
Table 1. Comparison of PCV-4200, PCV-3000 and PCV-6000
Multi-Spin
PCV -6000
6000 rpm
2350 x g
15 sec
1 min
Specifications
Speed range max. RCF max. Number of tubes vortexing
Time for completing “Spin-Mix-Spin” cycle:
for 2 microtubes for 12 microtubes for 100 microtubes
Combispin
PCV-2400
2800 rpm 3500 rpm
700 x g
1 individually
60 sec
5–6 min
60 min 15 min 10 min
Multi-Spin
PCV-3000
800 x g
12 simultaneously
25 sec
1 min 30 sec
PCR reaction preparation times have traditionally been lengthy, the Multi-Spin reduces this time. Trying to achieve the same sms algorithm manually is around ten times slower (up to 30 min for 100 samples vs 30 min for 1000 samples on the Multi-Spin) and the reproducibility of results may vary due to slight differences in the vortex and centrifugation stages, a normal consequence of manual operations.
Multi-spin PCV-3000
Operating Instructions
PCV-3000 Multi -Spin is four devices combined in one:
• multitube centrifuge.
• multitube vortex (3 modes - soft, medium, hard; regulated time; Vortexing regulation timer 1–20 sec).
• multitube centrifuge/vortexer.
• centrifuge/vortex/centrifuge cycler for realisation of the SMS algorithm.
Areas of application:
Reproducible Multi-tubes Vortexing ® Reproducible Spin-Mix-Spin cycling ® PCR samples premixing before thermo cycling ® Micro sampling before enzymatic reaction ® Cells permeabilization by chelating or hydrophobic agents for reaction in situ ® Low solubility Drug testing ® Cells washing from culture media after fermentation ® Preparing a sample before loading into a gel for Electrophoresis ® Magnetic beads
technology.
Multi-spin PCV-3000 Operating Instructions
Version 2.02 - August 2012

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3. Getting started
3.1 Unpacking
3.2 set includes:
3.3 Set up:
Remove packing materials carefully, and retain for future shipment or storage of the unit.
Complete set. The unit Standard set:
• Centrifuge/Vortexer Multi-spin PCV-3000 ....................................................... 1
•
External power supply ......................................................................................1 pce.
•
Rotor fixation pin...............................................................................................1 pce.
•
rotor 18091 u...................................................................................................1 pce.
•
rotor 18092 v...................................................................................................1 pce.
•
Operating Instructions; Declaration of Conformity ...........................................1 copy
Optional accessories:
•
rotor R2-05 w............................................................................................on request
•
rotor R2-05-02 x.......................................................................................on request
•
rotor PSR-16 y.........................................................................................on request
-place the unit on the horizontal even working surface;
-plug the external power supply unit into the 12 V socket at the rear side of the unit.
-it is necessary to observe the safety area of 300 mm around the unit in accordance
with EN-61010-2-20. Persons and hazardous materials must not be located in the safety area whilst the centrifuge is in operation.
pce.
Multi-spin PCV-3000
Operating Instructions
3.4 Rotor replacement:
To change a rotor (Fig.1/v): insert the provided pin into the opening in the fixation head (Fig.1/u). Hold the rotor with one hand and turn the fixation head (using the pin as a lever) counter clockwise to set rotor free. Change the rotor and fix it properly putting back the fixation head.
Fig.1 Rotor replacement
Multi-spin PCV-3000 Operating Instructions
Version 2.02 - August 2012

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4. Operation of PCV-3000
3000 HARD OFF
0:00 OFF STOP
Fig.1 Control panel
Recommendation during operation
Always load the rotor evenly. To keep the rotor balanced insert EVEN number of equal volume tubes in rotor sockets facing one another.
2 TUBE
ARRANGEMENT
4.1. Connect the external power supply to the mains.
4.2. Open the lid and place EVEN number of tubes in rotor sockets facing one another. Close the lid.
4.3. Parameter setting: Press Select key (Fig.2/w) to choose the parameter to change (each press
of the Select key will sequentially activate the parameters in the cycle, the active parameter is blinking).
4.4. Use “▲” and “▼“ keys (Fig.2/z) to set the necessary value (note: if the key is pressed for more
4 TUBE
ARRANGEMENT
6 TUBE
ARRANGEMENT
FULL
ARRANGEMENT
Multi-spin PCV-3000
Operating Instructions
that 2 sec the number changes quickly).
4.5. The program can also be changed during the operation - microprocessor automatically enters the last changes into the memory as the working program when the new cycle begins.
4.6. SMS - algorithm
4.6.4. Set the necessary speed of spin (increment 100 RPM, Fig.2/u).
4.6.5. Set the time of spin (time less than 1 min - with 1 sec increment, more than 1 min - with 1 min increment, Fig.2/v).
4.6.6. Set the vortexing strength (select from soft, medium and hard, Fig.2/x).
4.6.7. Set the time of vortexing, from 1 to 20 sec (increment 1 sec, Fig.2/y).
4.6.8. Set how many times the set sms - algorithm shall be repeated (from 1 till 999 times,
Fig.2/{).
4.7. Multitube centrifugation
4.7.1. Set the necessary speed of spin (increment 100 RPM, Fig.2/u).
4.7.2. Set the time of spin (time less than 1 min - with 1 sec step, more than 1 min - with 1
min increment, Fig.2/v).
4.7.3. Turn off the Vortex type motion by setting the time of Vortex type motion to zero
(OFF, Fig. 2/y). Note that the cycle counter turns off.
4.8. Multitube vortexing
4.8.1. Turn off the Spin motion by setting the time of Spin motion to zero (OFF, Fig. 2/v).
Note that the cycle counter turns off.
4.8.3. Set the vortexing strength (select from soft, medium and hard, Fig.2/x).
4.8.4. Set the time of vortexing (from 1 till 20 sec, increment 1 sec, Fig.2/y).
4.9. Press Run/Stop key (Fig.2/}) to start the program.
4.10. The rotor motion begins and the corresponding indication (RUN (Fig.2/|), cycle countdown (Fig.2/{), the changing time values (Fig.2/v or Fig.2/y)) is shown on the display.
4.11. Multi-Spin automatically stops after the set number of cycles is performed (blinking indication STOP (fig.2/|) on the display) and gives a sound signal about the end of operation (press Run\Stop key to stop the signal).
4.12. For the repeated operation according to the set program press Run\Stop key.
4.13. If necessary Multi-Spin can be stopped at any time during operation before the set number of cycles is performed by pressing Run/Stop key. Pressing Run/Stop key again will start the program from the beginning (cycle countdown will be restarted).
4.14. At the and of operation unplug the external power supply from the mains.
4.15. Post session cleaning
It is important to thoroughly clean all parts of the PCV-3000 that may have been contaminated by
the previous operation. This can be done by using isopropyl alcohol (up to 75%) or hot soapy water.
Multi-spin PCV-3000 Operating Instructions
Version 2.02 - August 2012

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5. Specifications
The product is designed for operation indoors in a laboratory at altitudes up to 2000m, with ambient temperature from +4°C to +40°C and maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.
• Spin rotation ...........................................................................
• Max. G-force ..............................................................................................................................800
• Spin timer .................................................................................................................1 sec - 99 min
• Vortexing strength ............................................................................................soft, medium, hard
• Vortexing time .......................................................................................0-20 sec (1 second steps)
• Cycle regulation .........................................................................................................1-999 cycles
Safety ..............................................................................................................Autostop at open lid
•
Input current/power consumption ..................................................................12V, 900 mA / 11W
•
External power supply ............................................input AC 100-240 V 50/60Hz, output DC 12V
•
• Dimensions ........................................................................................................190х235х125 mm
• Weight with power supply, not more ..................................................................................2,3 kg
Optional accessories
for 8 x 2.0 ml + 8 x 0.5 ml microtest tubesrotor R2-05
for 6x2,0 ml + 6x0.5 ml + 6x0.2 ml microtest tubesrotor R2-05-02
for 2 strips 8 x 0,2 ml microtubesrotor PSR16
for 12 x 1.5 ml microtest tubesrotor 18091
for 12 x 0.5 + 12 x 0.2 ml microtest tubesrotor 18092
Grant is committed to a continuous programme of improvement, specifications may be changed without notice.
1000 - 3500 RPM (100 RPM steps)
Description
DescriptionReplacement parts
Multi-spin PCV-3000
Operating Instructions
6. Guarantee and service
6.1. Guarantee
6.2. Service & Maintenance
6.3. Cleaning & Disinfection
When used in laboratory conditions and according to these working instructions, this product is guaranteed for TWO YEARS against faulty materials or workmanship.
There are no user-serviceable parts inside the unit. For all maintenance and repairs
return to our service department in the UK or in other countries,our distributor.
Standard ethanol (75%) or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and disinfection of the unit.
Multi-spin PCV-3000 Operating Instructions
Version 2.02 - August 2012

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Multi-spin PCV-3000
Operating Instructions
Multi-spin PCV-3000 Operating Instructions
Version 2.02 - August 2012

Page 15

Grant Instruments (Cambridge) Ltd
Shepreth Cambridgeshire SG8 6GB UK Tel: +44 (0) 1763 260811 Fax: +44 (0) 1763 262410 Email: scientificsales@grantinstruments.com www.grantinstruments.com
Multi-spin/PCV-3000/17995/2.02
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