Grafco User Manual User Manual

Hematocrit Centrifuge

Model 410E

User Manual

Read this manual before operating the 410E Hematocrit
Centrifuge. Save this manual for future reference.

410E-INS-LAB-RevC15

CONTENTS

INTRODUCTION ................................................................................................... 2

PRODUCT OVERVIEW ................................................................................... 3

GLOSSARY ..................................................................................................... 3

INTENDED USE .............................................................................................. 4

IMPORTANT SAFETY GUIDELINES—PLEASE READ BEFORE USE .............. 4

SETUP ................................................................................................................... 5

UNPACKING ................................................................................................... 5

CONTENTS ..................................................................................................... 6

REPLACEMENT PARTS ................................................................................ 6

INSTALLATION ............................................................................................... 6

SPECIMEN COLLECTION ...................................................................................7

MATERIALS NEEDED: ................................................................................... 7

CAPILLARY TUBE SPECIFICATIONS ........................................................... 7

TUBE PREPARATION ....................................................................................7

OPERATION .........................................................................................................7

BALANCED LOADS ....................................................................................... 7

LOADING THE ROTOR .................................................................................. 8

SETTING THE DIGITAL TIMER ...................................................................... 8

OPERATING THE CENTRIFUGE ................................................................... 9

INTERPRETING RESULTS: USING THE READING SCALE .............................. 9

EXPECTED VALUE ...................................................................................... 10

LIMITATIONS AND FACTORS AFFECTING RESULTS............................... 10

DISASSEMBLY ................................................................................................... 11

TROUBLESHOOTING ........................................................................................ 11

MAINTENANCE .................................................................................................. 12

CLEANING .................................................................................................... 12

TRANSPORTATION ...................................................................................... 13

STORAGE ..................................................................................................... 13

SPECIFICATIONS ............................................................................................... 14

LIMITED WARRANTY ........................................................................................ 15

GF Health Products, Inc. is not responsible for typographical errors. For the most
updated and current information on packaging, warranties, products and specications,
including the most current version of these instructions, please visit our website
at www.grahameld.com.

GF Health Products, Inc.

Graham-Field and Grafco are registered trademarks of

INTRODUCTION

Your Grafco Hematocrit Centrifuge has been designed to
be reliable, safe, and easy to use. This manual contains
important operation and safety information. Carefully
read and understand the contents of this manual before
installing or using the Grafco Hematocrit Centrifuge.
Always install this centrifuge in accordance with local
electrical parameters.

410E-INS-LAB-RevC15 2

Product Overview

digital display

brake

switch

timer set button

(ten minute

increments)

spin start button

410E Hematocrit Centrifuge

lidtimer LED

locking
button

unlocking
button

ON/OFF
switch

timer set button
(one minute increments)

The Grafco Hematocrit Centrifuge, shown above, features a
digital timer (up to 99 minutes), xed speed, brake control,
and a reading scale. This unit spins loads at a xed speed
of 12,000 RPM and has a thermal cutout switch to prevent
overheated operating temperature. The Grafco Hematocrit
Centrifuge can hold up to 24 capillary tubes of size 1.2mm
(or 1.5mm) x 75mm.

Centrifugation of anticoagulated whole blood results in the
packing of red blood cells. The hematocrit reading is the
percentage of the volume of packed red cells in relation
to the volume of whole blood. The hematocrit tube is
lled with anticoagulated whole blood, one end sealed by
heat or insertion into a sealing clay, and then centrifuged.
Interpretation of the resulting Volume of Packed Red Cells
(VPRC) may be used to detect the presence of anemia or
polycythemia.

Glossary

Hematocrit Centrifuge: An instrument which uses
centrifugation to measure the ratio of the volume of red
blood cells to the total volume of blood.

G Force: The measurement for samples undergoing the
stress of acceleration in a centrifuge.

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RCF (Relative Centrifugal Force): The centrifugal force
that the samples undergo, which relies on the speed
of rotation (N) in RPM and the rotating radius (R). The
rotating radius is measured from the center axis of the
rotor to the extreme ends of the centrifuge tubes.
RCF Formula: RCF = 11.18 x R x (N/1000)²

Intended use

The Grafco Hematocrit Centrifuge is intended to provide
laboratory tests for hematocrit values (HCT) in the elds
of biochemistry, microbic immunity genetics, and blood
separation, as well as general clinic tests.

IMPORTANT SAFETY GUIDELINES—PLEASE
READ BEFORE USE

The safety statements presented in this chapter refer to the
basic safety information that the operator of the centrifuge
shall pay attention to and abide by. There are additional safety
statements in other chapters or sections, which may be the
same as or similar to the following, or specic to the operations.

WARNING: Indicates a potential hazard situation or

unsafe practice that, if not avoided, could result in

death or serious personal injury.

CAUTION: Indicates a potential hazard or unsafe

practice that, if not avoided, could result in moderate

or minor personal injury.

s NOTICE: Indicates a potential hazard or unsafe

practice that, if not avoided, could result in product or

property damage.

Info: Provides application recommendations or other useful
information to ensure that you get the most from your product.

WARNING: Important! Read and understand these

instructions before installing or using the Grafco

410E Hematocrit Centrifuge. If you do not understand

any part of these instructions, contact your medical

professional or GF authorized distributor for direction

in the use of this product.

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WARNING: For the safety of both the operator

and service personnel, take care when handling

substances that are known to be toxic, radioactive or

contaminated with pathogenic microorganisms when

using this centrifuge.

WARNING: If components are damaged or missing,

contact your GF authorized distributor immediately.

DO NOT use substitute parts.

WARNING: Notice for California Customers- California

Proposition 65 WARNING: This product contains a

chemical known to the State of California to cause

cancer and reproductive or developmental harm.

WARNING: GF Health Products, Inc. assumes no

responsibility for any damage or injury caused by

improper installation or use of this product.

SETUP

Unpacking

1. Check for obvious damage to the carton or its contents.

If damage is evident, please notify the carrier and GF

Health Products, Inc.

2. Open the product box carefully. This may include

cutting external packaging strips which surround the

product shipping box. Also, for added safety when

shipping, the centrifuge may sometimes be packaged

inside the outer shipping box, meaning two boxes must

be opened.

3. Carefully remove the Hematocrit Centrifuge from the

packaging, including removing the plastic bag in which

the centrifuge is placed for shipping purposes. Do not

drop the centrifuge. Ensure that all of the equipment

listed in Contents, following, is present.

4. Keep the packing materials for transportation and/or

storage.

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Contents

Your Grafco Hematocrit Centrifuge contains:

Equipment
supplied
with 410E

Quantity Description

1 Rotor with lid

1 Detachable power cord

1 Reader scale

Replacement parts

Replacement parts available for the Grafco 410E
Hematocrit Centrifuge (from your GF authorized
distributor): Grafco Item 410E-1, Replacement Carbon
Brushes, package of two.

Installation

s NOTICE: The operation of AC motor is affected by

the surrounding power consumption, which will

result in a uctuation of speed. To assure an accurate

RPM and its corresponding RCF, do not place this

equipment in draft, sunlight or near a piece of

equipment that emits heat and/or electromagnetic

conduction emission.

1. Place the centrifuge in a large, open space with

unencumbered air ow on a sturdy, at, level countertop

with sufcient clearance to open the lid completely.

2. Attach the product's electrical cord to the cord port

located on the back of the centrifuge.

3. Plug the centrifuge's power cord into a properly

grounded (110V / 60Hz) electrical wall outlet.

s NOTICE: Locate the unit so that the centrifuge will

not be accidentally pulled or dislodged from the

countertop; and so the cord will not be stepped on,

tripped over, or otherwise subjected to damage or

stress.

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SPECIMEN COLLECTION

Materials Needed:

Capillary tubes If non-anticoagulated blood is used for the procedure, we

recommend the use of heparinized capillary tubes
If blood is already anticoagulated, we recommend the use of

plain capillary tubes

Clay sealant

Centrifuge with rotor which will accept capillary tubes and attain at least
11,500 RPM

Chart for determining hematocrit values

Capillary Tube Specications

Capillary Tubes Heparinized:

Plain

I.D. 1.1mm – 1.2mm, wall 0.2mm + 0.02mm,
length 75mm

Precalibrated:
Heparinized

Calibration line 60mm from one end

I.D. 0.5mm – 0.6mm, O.D. 1.4mm – 1.7mm,
length 75mm

Tube Preparation

1. Using 2 capillary tubes, insert an end of each into the

blood, tilt slightly and allow the tubes to ll by capillary

attraction to 2/3 – 3/4 full.

2.

Create a sealing plug: Insert the dry ends of the tubes into

the sealing clay and withdraw them with a twisting motion.

OPERATION

Balanced Loads

s NOTICE: Your centrifuge must contain a balanced

load in order to work properly. Spinning unbalanced

loads may shorten the life of the device and produce

unpredictable results. Observe the following rules

when loading the rotor.

• Opposingrotorgroovesmustbeemptyorloadedwith

equally weighted samples.

• Ifanoddnumberofsamplesistobespun,llatube

with water to match the weight of the unpaired sample

and place it across from the unpaired sample.

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Loading the Rotor

The Grafco Hematocrit Centrifuge can hold up to 24
capillary tubes of size 1.2mm (or 1.5mm) x 75mm.

1. Open the centrifuge lid (if centrifuge lid is locked, press

the unlocking button on front of centrifuge to unlock it).

2. Turn the rotor lid knob counter-clockwise to unlock the

it. Open the rotor lid.

3. Carefully place the test tubes in the rotor grooves,

ensuring the capillary tubes touch the rotor's outer

rubber gasket.

4. Make sure the load is balanced by placing similar tubes

across from one another. This will keep the rotor in

balance (see previous section, Balanced Loads).

5. Close the rotor lid gently. Turn the knob clockwise to

lock it.

WARNING: Closing the rotor lid improperly may result

in broken capillary tubes, which could cause injury.

6. Close the centrifuge lid completely. Press the locking

button on the side of the centrifuge to lock the lid.

7. Turn the ON/OFF switch to ON position.

Setting the Digital Timer

• TheGrafcoHematocritCentrifugeisequippedwithan

LED digital display timer which allows the user to spin

the load from 1 to 99 minutes.

• Pressthetriangledirectlybelowtheleftsideofthe

timer to change the spin time in increments of ten (10)

minutes.

• Pressthetriangledirectlybelowtherightsideofthe

timer to change the spin time in single minutes.

• Forexample,ifthedesiredspintimeistwentyve

minutes, press the left arrow twice to display a 2 on the

timer and press the right arrow ve times to display a

ve on the timer.

410E-INS-LAB-RevC15 8

Operating the Centrifuge

WARNING: As a safety feature, the centrifuge

has a power shut-off device to turn the power off

automatically when the lid is opened during spinning.

This is a safety feature of the centrifuge. DO NOT

open the lid when the rotor is spinning.

1. After the desired spin time is displayed on the timer,

press the START button to initiate rotor spinning.

2. The timer will display the spin time remaining during

operation. When the spin cycle is complete, the unit will

slowly stop spinning.

3.

To accelerate the deceleration, press the BRAKE SWITCH.

4. Once the rotor has completely stopped spinning, turn

the ON/OFF switch to OFF position.

5. Press the unlocking button located on the front of the

centrifuge to unlock the lid. Open the centrifuge lid.

6. Carefully open the rotor lid and remove the specimens.

WARNING: DO NOT open the centrifuge lid until the

rotor has completely stopped spinning. Opening the

lid before the rotor has stopped spinning could result

in personal injury.

INTERPRETING RESULTS: USING THE READING
SCALE

100%

90

80

tube

holder

capillary

tube

410E-INS-LAB-RevC15 9

diagonal

line

70

60

50

40

30

20

10

0

centrifuge

adjustablebar

capillary tube

extremeend

leucocyte

demarcation

redbloodcells

scale

1. Remove the capillary tubes from the rotor. Take care

not to let the blood drain from the tubes.

2. Place the capillary tubes on the grooves of the tube

holder carefully. Line up the bottom red blood cell

portion of the tube with the straight line at the bottom

of the reading scale so that the start of the red blood

cell portion lines up with this horizontal xed line.

3. Move the tube holder from left to right on the reader

until the top of the leucocyte line matches up with the

xed diagonal line on the reader platform.

4. Move the adjustable bar to meet the demarcation (place

where the leucocyte and red blood cells separate). The

diagonal line on the adjustable bar should be directly on

top of the demarcation.

5. The percentage of HCT can then be read directly from

the scale by reading the number the adjustable diagonal

bar crosses at the right side of the reader.

Expected Value

Normal hematocrit values will vary somewhat depending
upon such factors as age, sex, size of red cells, and
altitude. Generally accepted normal values with range of
variation are:

 • Women:36%–46%

• Men:42%–52%

Limitations and Factors Affecting Results

1. Inadequate centrifugation, either time or RPM, will

result in erroneously high reading.

2. Ensure that the capillary tube is sealed properly, to

prevent red cell loss during centrifugation. The rubber

gasket encircling the interior of the rotor must be

smooth and pliable. This will aid in clay sealing plug

retention and reduce breakage during centrifugation.

3. Take the reading from the top of the clay sealing plug to

the top of the column of VPRC.

410E-INS-LAB-RevC15 10

4. Hemolized blood, due to over-anticoagulation or

improper nger stick, can produce erroneous results.

5. Hematocrit determinations are not accurate

immediately following blood transfusions or blood loss.

DISASSEMBLY

WARNING: Disassembly or dismantling of this

centrifuge is limited to authorized service personnel

only. Dismantling or disassembly by non-qualied

personnel will result in the voiding of the unit’s

warranty. Please contact your GF authorized

distributor or customer service representative to

identify a qualied technician.

TROUBLESHOOTING

WARNING: Unplug the centrifuge before performing

troubleshooting corrective actions.

WARNING: Do not disassemble or dismantle the

centrifuge.

Problem Possible cause Corrective action

Can’t turn
centrifuge
ON

Erratic
speed

Vibration Unbalanced rotor Balance rotor load

Imbalance 1. Tubes are improperly

Broken test
tubes

1. Blown fuse

2. Disconnected electrical
cord

3. Worn carbon brushes

Worn carbon brushes Contact GF authorized distributor

positioned (unbalanced load)

2. Rotor lid is not locked

1. Tubes are improperly
positioned (unbalanced load)

2. Tubes are not placed all the
way down

3. Rotor lid is tightened
incorrectly

1. Contact GF authorized
distributor to replace fuse (10A)

2. Connect electrical cord

3. Contact GF authorized distributor
to replace carbon brushes

to replace carbon brushes

1. Balance load

2. Screw rotor knob to lock lid

1. Balance load

2. Place tubes against rubber
gasket on rotor

3. Ensure rotor lid is placed at

against rotor

410E-INS-LAB-RevC15 11

MAINTENANCE

WARNING: Centrifuge disassembly or dismantling is

limited to authorized service personnel only.

WARNING: Always use a properly grounded electrical

outlet of correct voltage and current handling capacity.

WARNING: Do not immerse the centrifuge in or

saturate it with water.

s NOTICE: Do not lubricate the drive motor or linkage

assembly.

s NOTICE: Do not drop the centrifuge, turn it upside

down, or subject it to vibration.

s NOTICE: Clean the chamber and rotor as described

below after each use.

s NOTICE:

In order to spin balanced loads, the

centrifuge must be level. To this end, conrm the

rubber supports are in good condition before each use.

s NOTICE: Contact your GF authorized distributor to

replace the carbon brushes yearly or as needed.

Cleaning

Clean the equipment as described below after each use.

1. Unplug the unit and disconnect the power cord from the
unit.

2. Clean the outer case, rotor lid, and rotor tube holder
grooves with a moist, clean cloth.

3. Ensure the equipment is thoroughly dry. Attach the
product's electrical cord to the cord port located on the
back of the centrifuge.

4. Plug the centrifuge's power cord into a properly
grounded (110V / 60Hz) electrical wall outlet.

s NOTICE: DO NOT use benzine or paint thinner for

cleaning.

410E-INS-LAB-RevC15 12

Transportation

s NOTICE: When transporting the unit, place in secure

packaging (preferably the original packaging material)
which will prevent the unit from tipping over.

s NOTICE: Do not drop the centrifuge, turn it upside

down, or subject it to vibration.

s NOTICE: Transport within temperature limitations

listed in following SPECIFICATIONS section.

Storage

s NOTICE: Do not place this equipment in a draft,

sunlight or near a piece of equipment which emits
heat or electromagnetic conduction emission.

s NOTICE: Disconnect power supply when the

centrifuge is not in use.

s NOTICE: DO NOT place other equipment or objects on

top of the centrifuge.

s NOTICE: Store within temperature limitations listed in

following SPECIFICATIONS section.

410E-INS-LAB-RevC15 13

SPECIFICATIONS

Model Grafco 410E Hematocrit Centrifuge

CE marking Conforms to LVD 73/23 EEC safety standards.

Standard Complies with EC Electromagnetic Compatibility requirement

of EN60601-1-2.

EMI lter is designed in conformity with 89/336 EEC-EMC Directive.

Complies with IEC801 series regulations.

Complies with EN standard

ISO9001, ISO13485, GMP

N.W. 25.35 lb (11.5 kg)

Timer Digital, 1 to 99 minutes

Motor input Brush 80W

Brake Manual

Rotor F-2415

Capacity 24 capillary tubes

Max RCF 14490 x g

Speed RPM Fixed 12,000 (though xed at 12,000 RPM, motor is capable of

14,000 RPM)

Capillary tube size 1.1 – 1.2mm x 75 mm (length)

Conditions for
Transportation
and Storage

410E-INS-LAB-RevC15 14

Temperature: 50ºF – 104ºF (10ºC – 40ºC)

Humidity: 40% – 90%

LIMITED WARRANTY

SCOPE OF WARRANTY

GF Health Products, Inc. (“GF”) warrants to the original purchaser only that it will replace or
repair components, at GF’s sole discretion, that are defective in material or workmanship under
normal use and service. All warranties are conditioned upon the proper use of the products
strictly in accordance with good commercial practice and applicable GF instructions and
manuals, including proper use and maintenance. To the extent that a component is warranted
by a third party, GF conveys all of its rights under that warranty to the original purchaser, to
the extent permitted. This limited warranty shall only apply to defects that are reported to GF’s
customer service team within the applicable warranty period and which, upon examination by
GF or its authorized representative, prove to be a warranty item. This limited warranty is not
transferable. Within the guidelines set forth in this document, this product is warranted for one
(1) year. The applicable warranty period shall commence from date of shipment to the original
customer, unless there is an expiration date on the component in which case the warranty shall
expire on the earlier of warranty period or the expiration date.

OBTAINING WARRANTY SERVICE

This limited warranty shall only apply to defects that are reported to the Distributor from whom the
Customer purchased the product within the applicable warranty period. If there is not a Distributor,
you must contact GF directly by calling 770-368-4700, sending a fax request to 770-368-2386, or by
e-mailing a request to cs@grahameld.com. Specic directions will be provided by the Customer
Service Representative. Failure to abide by the specic directions will result in denial of the
warranty claim.

EXCLUSIONS

The warranty does not cover and GF shall not be liable for the following:

1) Defects, damage, or other conditions caused, in whole or in part, by misuse, abuse,

negligence, alteration, accident, freight damage, tampering or failure to seek and obtain repair
or replacement in a timely manner;

2) Products which are not installed, used, or properly cleaned and maintained as required in the

ofcial manual for the applicable product;

3) Products considered to be of a non-durable nature including, but not limited to: casters,

lters, fuses, gaskets, lubricants, and charts;

4) Accessories or parts not provided by GF;

5) Charges by anyone for adjustments, repairs, replacement parts, installation or other work

performed upon or in connection with such products which are not expressly authorized in
writing, in advance, by GF;

6) Any labor or shipping charges incurred in the replacement part installation or repair;

7) Costs and expenses of regular maintenance and cleaning; and

8) Representations and warranties made by any person or entity other than GF.

ENTIRE WARRANTY, EXCLUSIVE REMEDY AND CONSEQUENTIAL DAMAGES
DISCLAIMER

THIS WARRANTY IS GF’S ONLY WARRANTY AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESS
OR IMPLIED. GF MAKES NO IMPLIED WARRANTIES OF ANY KIND INCLUDING ANY IMPLIED
WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IF ANY MODEL
OR SAMPLE WAS SHOWN TO THE CUSTOMER, SUCH MODEL OR SAMPLE WAS USED MERELY TO
ILLUSTRATE THE GENERAL TYPE AND QUALITY OF THE PRODUCT AND NOT TO REPRESENT
THAT THE PRODUCT WOULD NECESSARILY CONFORM TO THE MODEL OR SAMPLE IN ALL
RESPECTS. THIS WARRANTY IS LIMITED TO THE REPAIR OR REPLACEMENT OF THE DEFECTIVE
PARTS. GF SHALL NOT BE LIABLE FOR AND HEREBY DISCLAIMS ANY DIRECT, SPECIAL, INDIRECT,
INCIDENTAL, EXEMPLARY OR CONSEQUENTIAL DAMAGES, INCLUDING, BUT NOT LIMITED TO:
DAMAGES FOR LOSS OF PROFITS OR INCOME, LOSS OF USE, DOWNTIME, COVER, OR EMPLOYEE
OR INDEPENDENT CONTRACTOR WAGES, PAYMENTS AND BENEFITS.

The warranties contained herein contain all the representations and warranties with respect
to the subject matter of this document, and supersede all prior negotiations, agreements and
understandings with respect thereto. The recipient of this document hereby acknowledges and
represents that it has not relied on any representation, assertion, guarantee, warranty, collateral
contract or other assurance, except those set out in this document.

For additional information on this product or this warranty, please contact a GF Customer Service
Representative.

NOTES:

1) Additional terms and conditions may apply.

2) Freight claims must be notated on the appropriate shipping documents and must be made

with immediacy. International, federal and state regulations govern specic requirements for
freight claims. Failure to abide by those regulations may result in a denial of the freight claim.
GF will assist you in ling the freight claim.

3) Claims for any short shipment must be made within three (3) days of the invoice date.

410E-INS-LAB-RevC15 15

Made in Taiwan

© 2008 GF Health Products, Inc.