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INTRODUCTION OF THE INHALATION EXPOSURE SYSTEM (IES):
The Inhalation Exposure System (IES) is an instrument manufactured by Glas-Col that provides
reproducible animal exposure to droplet-borne contaminates and infectious agents. It produces
deep-lung infections that more closely simulate real-world circumstances.
The system allows you to vary the concentration of airborne pathogens, the rate of airflow and
the length of exposure, so experiments can be reproduced accurately. The system is a wholebody expose chamber for the quantitative infection of animals by inhalation of air bearing the
pathogenic agent.
The heart of the system is the nebulizer-venturi unit into which the suspension is introduced.
Air under pressure atomizes the suspension, which is mixed with filtered room air drawn into
the system by a suction beyond the decontaminate system. By varying the number of microorganisms in the suspension, the proportion of bacteria-bearing nuclei can be varied. The
system permits control of the volume of air moving through the chamber per unit of time.
The animals are placed in a compartment mesh basket within the chamber. Compressed air and
vacuum pumps, along with the necessary controls and flow indicators, are built into the system.
The entire unit is always operated under negative pressure (vacuum). A micro controller is
used to control the preheat, nebulizing, cloud decay, and decontamination periods. A Hepa
filter and ultraviolet lamps are provided for cleaning the system.
Below in Figure 1, is the Inhalation Exposure System diagramed in the present state.
Figure 1. Diagram of Inhalation Exposure System
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UNPACKING:
The Inhalation Exposure System is normally shipped via truck freight or by movers
experienced in handling delicate equipment.
1. Open the door and carefully remove the contents of the chamber. Remove and
discard empty cartons used as spacers and packing material wedged around the
basket. Carefully lift the basket out of the chamber
2. The NEBULIZER AND ULTRAVIOLENT LAMP is secured inside the basket
compartments.
3. The power cable is coiled in user instruction bag.
INSTALLATION:
The Inhalation Exposure System is shipped with all of the components mounted in place
and ready to operate with the exception of the nebulizer-venturi unit and the ultra-violet
lamp.
The U.V. lamp will mount in the trough of the aluminum reflector on the underside of the
lid.
With the lid fully open, remove the nut and plastic strip on the threaded mounting studs
that protrude on one end of each aluminum reflector. Insert the pins on the U.V. lamp
into the socket that is mounted to the opposite end of the reflector. Slip the U-shaped
ultraviolet lamp over the stud and then the nut. Tighten gently so that the plastic strip
holds the lamp firmly but without excessive pressure. Use caution not to touch the UV
bulb unless wearing gloves.
NEBULIZER-VENTURE UNIT:
The nebulizer-venturi unit comes ready to mount to the front panel. Three stainless steel
sockets are located at the bottom edge of the front panel. In order to insure good
alignment it may be necessary to move the sockets right or left or up or down. Place the
nebulizer so the appropriate ports align with the appropriate sockets. Once satisfied that
the fit is good, place a pinch clamp over each of the ball and socket connections. Tighten
the knurled nut on each pinch clamp to ensure a leak-free seal. Refer to figure 2.
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Figure 2. Nebulizer Connection
EXTERNAL CONNECTIONS:
Connect the power cable to a 3-prong, grounded 120v, 60Hz, or 240v, 50/60Hz
(depending on model) AC voltage source.
SPECIFICATIONS FOR EACH COMPONENT:
LID:
A 5/8" thick acrylic plastic lid, impervious to ultraviolet rays covers the chamber. Three
latches provide sufficient pressure on the gasket to seal the lid to the tank.
To open, pull out on each latch. To close, press firmly on the red handle until it snaps.
ULTRAVIOLET LAMPS:
One U-shaped ultraviolet lamp, with an intensity of 4 watts at 254 Nanometers; has a
rated life of 1000 hours.
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