Page 1
Conductivity and pH Monitor
User’s Guide
LT304013-02
www.gilson.com/ContactUs
Page 2
Page 3
Safety
Symbols and Signs..................................................................8
Safety Notices ......................................................................8
Solvents............................................................................8
Replacement Parts..................................................................8
Sécurité
Symboles et Signes ................................................................10
Notes de Sécurité..................................................................10
Solvants ...........................................................................10
Pièces détachées ..................................................................10
1 Introduction
TABLE OF CONTENTS
Description........................................................................12
Unpacking ........................................................................12
Standard Equipment...........................................................13
Accessories ....................................................................13
Technical Specications............................................................14
Customer Service ..................................................................16
2 Installation
Plumbing Connections ............................................................18
Rear Panel Connections............................................................19
pH Sensor .....................................................................19
Output Channels ..............................................................19
USB Device ....................................................................19
VERITY® 1810 Conductivity and pH Monitor | User’s Guide
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3 Operation
Front Panel ........................................................................22
Flow Path Diagram ................................................................22
Start Up ...........................................................................22
Table of Contents
Control from TRILUTION®LC........................................................23
Conguration..................................................................23
Control ........................................................................24
Analysis .......................................................................24
Application Run ...............................................................24
Results ........................................................................25
Temperature Compensation Coecient ............................................26
Conductivity Analog Endpoint .....................................................27
4 Maintenance
Cleaning ..........................................................................30
Exterior........................................................................30
Conductivity FlowCell .........................................................30
Conductivity Flow Cell .............................................................31
Calibration ....................................................................31
Replacement ..................................................................31
Inlet and/or Outlet Tubing Replacement........................................32
5 Troubleshooting
Troubleshooting...................................................................34
Solvent and Buer Compatibility ...................................................34
Chemical Resilience............................................................34
Repair and Return Policies .........................................................35
BeforeCallingUs...............................................................35
Warranty Repair ...............................................................35
Non-Warranty Repair...........................................................35
Return Procedure ..............................................................35
Unit End-of-Life................................................................35
Table of Contents | VERITY® 1810 Conductivity and pH Monitor
Page 5
A Replacement Parts and Accessories
VERITY® 1810 Conductivity and pH Monitor ........................................37
FlowCells .........................................................................37
Tubing and Fittings ............................................................37
pH Sensor .........................................................................38
Cables.............................................................................38
B pH Flow Cell
Technical Specications............................................................39
Setup .............................................................................40
Plumbing Connections ............................................................41
Connection to the VERITY® 1810 Conductivity and pH Monitor ......................42
pH Sensor .....................................................................42
Flow Path Diagram ................................................................42
Table of Contents
VERITY® 1810 Monitor pH Calibration Utility ........................................43
Install the VERITY® 1810 Monitor pH Calibration Utility ..........................43
Prepare to Run the VERITY® 1810 Monitor pH Calibration Utility .................43
Start the VERITY® 1810 Monitor pH Calibration Utility ...........................44
Use the VERITY® 1810 Monitor pH Calibration Utility ............................44
Close the VERITY® 1810 Monitor pH Calibration Utility...........................45
Maintenance ......................................................................46
pH Sensor Replacement........................................................46
VERITY® 1810 Conductivity and pH Monitor | User’s Guide
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Page 7
SAFETY
Read this chapter before installing and operating the instrument.
Only trained technical personnel in a laboratory environment may use the instrument for non-medical, liquid
handling purposes. For safe and correct use of the instrument, operating and service personnel must follow
all instructions contained in this guide when installing, cleaning, and maintaining the instrument. All safety
precautions must be observed during all phases of operation, service, and repair of the instrument.
Failure to comply with these precautions or with warnings described in the user’s guide violates safety
standards of design, manufacture, and intended use of the instrument. Gilson assumes no liability for
customers failing to comply with these requirements.
The instrument has been certified to safety standards required in Canada, Europe, and the United States.
Refer to the rear panel label on the instrument and the Declaration of Conformity document for the current
standards to which the instrument has been found compliant.
VERITY® 1810 Conductivity and pH Monitor | User’s Guide
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Page 8
Symbols and Signs
WARNING
CAUTION
NOTICE
Safety
The following table explains symbols and labels that are used on the instrument or in the user’s guide:
Symbol Meaning
Caution
Attention
Vorsicht
Safety Notices
The following safety notices may appear in this document:
WARNING indicates a potentially hazardous situation which, if not avoided, may result in
serious injury
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury
NOTICE indicates a potentially hazardous situation which, if not avoided, may result in
equipment damage
Solvents
Observe safe laboratory practices when handling solvents. If dangerous liquids are used, adequate
protection such as proper ventilation, safety glasses, etc., should be used.
Refer to the Material Safety Data Sheets for the solvents before use.
Replacement Parts
Be sure to use only replacement parts mentioned in this user’s guide.
8
Safety | VERITY® 1810 Conductivity and pH Monitor
Page 9
SÉCURITÉ
Merci de lire attentivement cette section avant toute installation ou utilisation de l’instrument.
Cet instrument est exclusivement destiné à être utilisé dans un environnement de laboratoire, par un
personnel qualifié, à des fins de manipulations de liquides non-médicales. Pour une utilisation correcte
et en toute sécurité de l’instrument, il est nécessaire que le personnel qui utilise et réalise la maintenance
de l’instrument, suive les instructions contenues dans ce guide lors de l’installation, du nettoyage et de la
maintenance de l’instrument. Toutes les consignes de sécurité doivent être respectées durant toutes les
phases de fonctionnement, d’entretien ou de réparation de l’instrument.
Le non-respect de ces précautions ou des avertissements spécifiques mentionnés dans ce guide compromet
les normes de sécurité de conception, de fabrication et d’utilisation prévue de l’instrument. Gilson décline
toute responsabilité en cas d’incapacité du client à se conformer à ces exigences.
L’instrument a été certifié conformément aux normes de sécurité en vigueur au Canada, en Europe et aux
Etats-Unis. Merci de vous reporter aux indications mentionnées sur le panneau arrière de l’instrument
ainsi qu’au document de Déclaration de Conformité aux normes pour lesquelles l’instrument a été déclaré
conforme.
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Symboles et Signes
WARNING
CAUTION
NOTICE
Sécurité
Le tableau suivant détaille les symboles et signes utilisés sur l’instrument ou dans le guide de l’utilisateur:
Symbole Signification
Caution
Attention
Vorsicht
Notes de Sécurité
Les notes de sécurité suivantes peuvent apparaitre dans ce document:
AVERTISSEMENT: indique une situation potentiellement dangereuse qui, si elle n’est pas
évitée, peut entraîner des blessures graves
ATTENTION: indique une situation potentiellement dangereuse qui, si elle n’est pas évitée,
peut entraîner des blessures mineures à modérées
AVIS: indique une situation potentiellement dangereuse qui, si elle n’est pas évitée, peut
entraîner des dégâts matériels
Solvants
Respectez les Bonnes Pratiques de Laboratoire lors de la manipulation de solvants. Si des liquides dangereux
sont utilisés, assurez-vous que la ventilation est adéquate et portez en permanence un équipement de
protection individuelle (EPI), tel que: lunettes, gants et vêtements de protection.
Reportez-vous aux Fiches de Données de Sécurité pour les solvants avant toute utilisation.
Pièces détachées
Assurez-vous de n‘utiliser exclusivement que les pièces détachées mentionnées dans ce guide.
10
Sécurité | VERITY® 1810 Conductivity and pH Monitor
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1 INTRODUCTION
Chapter One
This chapter provides information on the following topics:
● Description on page 12
● Unpacking on page 12
● Technical Specifications on page 14
● Customer Service on page 16
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Description
The VERITY® 1810 Conductivity and pH Monitor is a state-of-the-art detection instrument for biological
purifications with a large dynamic range and fast response time for real-time gradient monitoring. It is a
Description
compact, integral component for systems performing oligonucleotide, monoclonal antibody (mAb), and
other biological separations.
The VERITY® 1810 Conductivity and pH Monitor is a USB powered instrument with an integrated conductivity
sensor, an external connection for an optional pH sensor, and analog output connections for conductivity
and pH. Real-time conductivity and pH data can be viewed on an LCD display or monitored either through
the USB connection or analog outputs.
Figure 1
VERITY® 1810 Conductivity and pH Monitor with Optional pH Flow Cell
Unpacking
The instrument is delivered with most of the major components already assembled. Retain all packing
material so the instrument may be shipped safely in the future.
To unpack the instrument:
1. Open the box.
2. Remove the foam insert on the top of the instrument.
3. Grip the instrument and lift it out of the packaging.
4. Remove the instrument from the bag.
5. Remove the bag of accessories from the box.
12
Introduction | VERITY® 1810 Conductivity and pH Monitor
Page 13
Standard Equipment
● VERITY® 1810 Conductivity and pH Monitor with Installed Conductivity Flow Cell
● USB Cable
● Two Analog Cables
● Inlet and Outlet Fittings
● Gilson Ethernet Utility
Documentation
The following documentation is provided:
● VERITY® 1810 Conductivity and pH Monitor Documentation CD, which includes:
○ VERITY® 1810 Conductivity and pH Monitor User’s Guide
○ VERITY® 1810 Conductivity and pH Monitor Installation Qualication (IQ) Procedures
● Declaration of Conformity
Description
● Hazardous Materials Declaration (China RoHS)
● Conductivity Flow Cell Calibration Certificate
● Items Included Checklist
● Quality Control (QC) Checklist
Accessories
Required
Some accessories are required, but are ordered separately:
● Inlet and Outlet Tubing
● TRILUTION® LC Software v3.0 with Service Pack 6 (or higher)
Optional
● pH Flow Cell, which includes:
○ pH Sensor
○ pH Flow Cell Base
○ pH Calibration Utility CD
For more information, refer to the pH Flow Cell appendix in this user’s guide.
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Technical Specifications
NOTICE
Technical Specifications
Please be aware of the following before operating the instrument.
Changes or modications to the instrument not expressly approved by Gilson could void
the factory-authorized warranty.
The instrument complies with part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1) This instrument may not cause harmful interference, and (2) this instrument must accept any interference
received, including interference that may cause undesired operation.
Shielded cables must be used with the instrument to ensure compliance with the FCCClass A limits.
VERITY® 1810 Conductivity and pH Monitor
Technical
Specification
Analog Outputs Two 0–1V dedicated analog outputs: one for streaming conductivity data and the other for
Communication USB
Conductivity
FlowCell
Definition
streaming pH data
Accuracy
Part Number
Pressure
Readable
Range
Specification
Range
Wetted
Materials
± 4% or ± 20 µS/cm,whichever is greater,within 1 µS/cm–300 mS/cm at
25°C
11000002
psi bar MPa
200 13.79 1.38
1 µS/cm–999.99 mS/cm
1 µS/cm–300 mS/cm
PEEK, Titanium
14
Volume
Data Acquisition 2, 5, or 10 points/sec with TRILUTION® LC Software
Dimensions
(W x D x H)
Environmental
Conditions
Front Panel Three line display
Introduction | VERITY® 1810 Conductivity and pH Monitor
13.4 x 10.3 x 7.1 cm (5.3 x 4.1 x 2.8 in.)
Indoor use
Altitude: up to 2000 m
Temperature range: 4°C–40°C
Humidity: Maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly
to 50%relativehumidity at 40°C
54.1 µL
Page 15
VERITY® 1810 Conductivity and pH Monitor
Technical Specifications
Technical
Specification
Monitor Channels Three: Conductivity, pH, and Temperature
pH Flow Cell
Definition
Accuracy
Maximum
Drift
Part Number
Pressure
Readable
Range
Specification
Range
Wetted
Materials
±0.1 units at 25°C
0.1/10 hours at constant conditions
11100001
psi bar MPa
100 6.89 0.69
0–14 pH
2–12 pH
Acrylic, PVDF, pH Glass, epoxy, BUNA-N
Volume
Power
Requirements
Recommended
Flow Rate Range
Safety and
Compliance
Software Control PC control via USB and TRILUTION® LC Software
Weight 0.34 kg (0.75 lbs.)
USB powered instrument
Instrument Power Requirement: 5V DC (1W)
0.5–150 mL/min
The instrument has been certied to safety standards specied for Canada, Europe, and the
United States. Referto the instrument rear panel label and the Declaration of Conformity
document for the current standards to which the instrument has been found compliant.
110 µL
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Customer Service
Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following
types of assistance: sales, technical support, applications, and instrument repair.
If you need assistance, please contact your local Gilson representative. Specific contact information can be
found at www.gilson.com. To help us serve you quickly and efficiently, please refer to BeforeCallingUs on
Customer Service
page 35.
16
Introduction | VERITY® 1810 Conductivity and pH Monitor
Page 17
2 INSTALLATION
Chapter Two
This chapter explains the steps for setting up the instrument, which includes:
● Plumbing Connections on page 18
● Rear Panel Connections on page 19
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Plumbing Connections
NOTE
Inlet and outlet tubing are ordered separately so as to be appropriate for the application flow rate. Refer to
the Replacement Parts and Accessories appendix for part numbers. Fittings are supplied.
Plumbing Connections
To make the plumbing connections:
1. On each end of the tubing, slide the nut,
then the steel compression ring, and then
the ferrule. The attened end of the ring
should face towards the nut with the narrow
end of the ferrule towards the ring.
2. Insert the nut at each end into its port. (Ports
are not labeled as the direction of the liquid
flow is not specific.) Hold the tubing to the
bottom of the fitting while tightening the
nut to fingertight.
If the system includes a pH ow cell, one connection goes to the pH ow cell inlet. Refer to the
pH Flow Cell appendix for instructions and more information.
The flattened end of
the ring should face
towards the nut
The narrow
end of the
ferrule is to
touch the ring
Figure 2
Plumbing Connections
18
Figure 3
Plumbing Connections (Exploded View)
Installation | VERITY® 1810 Conductivity and pH Monitor
Page 19
Rear Panel Connections
NOTE
Refer to the diagram when making the connections
described in this section.
pH Sensor input
Analog pH output
Analog Conductivity output
USB Device port
pH Sensor
The pH sensor cable has a BNC connector.
After assembling the optional pH flow cell, connect the pH sensor
cable to the pH Sensor input, and then twist clockwise 1/4 turn to
lock. For pH flow cell assembly instructions, refer to the pH Flow Cell
appendix.
Rear Panel Connections
Figure 4
Rear Panel Diagram
Figure 5
BNC Connector on pH Sensor Cable
Output Channels
If the VERITY® 1810 Conductivity and pH Monitor is part of a Gilson system, it is not necessary
to make analog output connections. In a Gilson system, make the USB connection between the
VERITY® 1810 Conductivity and pH Monitor and the PC.
The monitor has two output channels: Analog pH and Analog Conductivity. Optionally, direct output from
the monitor to an external device.
To connect an output channel, use one of the supplied cables (part number 11000023). Connect the cable to
the corresponding output port and then twist the nut to secure the connection.
To set the analog output range, refer to Conductivity Analog Endpoint on page 27.
USB Device
The monitor communicates with a standard PC and is powered via
USB.
To make the USB connection between the
VERITY® 1810 Conductivity and pH Monitor and the controlling
device (PC) or power source (USBhub), use the USB cable
(partnumber 32000012) supplied in the accessory kit. Use the
end with the “A-type” (flat) connector to connect to the controlling
device or power source and use the end with the “B-type” (square)
connector to connect to the monitor.
Figure 6
USB Cable with “A-Type” Connector
Figure 7
USB Cable with “B-Type” Connector
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3 OPERATION
Chapter Three
Install TRILUTION®LC Software according to the installation instructions provided with the software. This
software provides control of the VERITY® 1810 Conductivity and pH Monitor. For more information about
TRILUTION®LC Software, refer to its user’s guide, on-line help, and documentation supplied with the
software.
For use in any HPLC system, use the analog outputs. Refer to Output Channels on page 19.
This chapter provides the following information:
● A description of the monitor Front Panel on page 22
● Flow Path Diagram on page 22
● Start Up on page 22
● Instructions for Control from TRILUTION®LC on page 23
● Instructions for viewing Results on page 25
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Front Panel
The front panel of the monitor contains a:
Front Panel
Three Line Display
○ The rst line displays the conductivity
channel reading.
○ The second line displays the pH channel
reading.
○ The third line displays the temperature
reading.
Figure 8
VERITY® 1810 Conductivity and pH Monitor
Front Panel Display
Flow Path Diagram
The flow to the VERITY® 1810 Conductivity and pH Monitor should be post column and post UV detector in
an HPLC system.
to waste or
fraction collector
from
column
Figure 9
Flow Path Diagram -
VERITY® 1810 Conductivity and pH Monitor with Conductivity Flow Cell, Optional pH Flow Cell, and
159 UV-VIS Detector
Start Up
Follow the instructions in the Installation chapter to make all rear panel and plumbing connections and then
start TRILUTION® LC software.
22
Operation | VERITY® 1810 Conductivity and pH Monitor
Page 23
Control from TRILUTION®LC
The following pages describe how to set conditions for control via TRILUTIONLC. For additional information
about method setup and the chromatography run in TRILUTIONLC, refer to the software on-line help or
user’s guide.
Configuration
The VERITY 1810 Conductivity and pH Monitor is added to TRILUTION® LC v3.0 after installation of
Service Pack 6.
The VERITY® 1810 Conductivity and pH Monitor will be
detected in a scan, which is the recommended way to add
an instrument to the configuration.
When adding the monitor to the configuration:
1. Optionally, type a unique description for each
datachannel. This description and the datachannel’s
signal will be displayed in the status box while data is
being collected during an Application Run if the Use
check box is selected for that datachannel. The Use
check box for 1810 Conductivity Channel defaults
selected. There are three data channels available:
Conductivity, pH, and Temperature.
Control from TRILUTION®LC
2. Right-click on a datachannel marked for use and then
select Primary Channel. One channel per conguration
must be specied as the primary channel. Fraction
collection and sample re-injection are based on the
primary channel.
3. Select the data rate, which is the number of data
points collected per second for all channels. One rate is
selected for all channels.
4. On the General tab, optionally enter a different name
that will be used to identify the monitor. (The default
name is Detector.)
5. Scanning for the monitor automatically populates the
serial number eld and is recommended, but typing
the serial number is also an option. The serial number is
located on the underside of the instrument.
Figure 10
Configuration Property Page (CPP) Setup Tab
Figure 11
Configuration Property Page (CPP) General Tab
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Control
The Control tabwindow in the MethodBuilder is a graphical interface used to organize the tasks used in a
method.
Tasks
The table below lists the tasks for the VERITY 1810 Conductivity and pH Monitor.
VERITY 1810 Conductivity and pH Monitor Tasks
Control from TRILUTION®LC
Task Icon Task Name and Description
Start Data Collection
This task tells TRILUTION LC to begin collecting data. This task has no properties to set.
Stop Data Collection
This task tells TRILUTION LC to stop collecting data. This task has no properties to set.
Error Handling
Optionally, use error handling to check the status of the instrument. Run a stop method to shut down the
system if an error is encountered.
Error Description
Instrument Error This error results when any scheduled command fails to execute or if the software loses
communication with an instrument.
Analysis
24
Using the options in the MethodBuilder - Analysiswindow, indicate how collected data is analyzed and
reported. TRILUTIONLC uses the information in the analysis to report on peaks detected in samples.
To create an analysis, right-click on a datachannel in the ConfiguredInstruments Panel and then select
NewAnalysis.
Application Run
In the Application Run window, specify the list of steps, called a sample list, to execute during a run. A step in
the sample list identifies the method to run. Access the Application Run window by:
● right-clicking an application in the ProjectLibrary and then selecting Run or
● selecting an application in the ProjectLibrary and then clicking Run or
● opening a saved method in the Method Builder and then clicking Run.
After setting up the sample list, click Run in the Application Run window to begin executing the steps in the
run.
Operation | VERITY® 1810 Conductivity and pH Monitor
Page 25
Results
From a run, the software produces results containing the channel data collected and stored for all injected
samples. Results contain time and signal information for each sample.
Results are accessed in the Project Library. Click on an application and double-click the results icon( ). The
Resultswindow is displayed, listing all runs for the selected application.
Double-click on a Run Name to display the Run Results window.
Run Results Window
The Run Results window enables viewing chromatogram plots and analysis information for collected data. To
view chromatogram results, preview the report.
Control from TRILUTION®LC
Figure 12
Run Results Window
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Temperature Compensation Coecient
For increased conductivity accuracy, the VERITY® 1810 Conductivity and pH Monitor includes the ability to
set a conductivity temperature compensation coefficient. Use the Set Temperature Compensation command
in Gilson Ethernet Utility. The temperature compensation coefficient varies by solution and by temperature.
By default, a 2% temperature compensation coefficient (Temperature Compensation = 0.02) is used by the
VERITY® 1810 Conductivity and pH Monitor.
To set a temperature compensation coefficient, use of the Gilson Ethernet Utility and the GEARS Application
is required. The Gilson VERITY 1810 Conductivity and pH Monitor GEARS Plugins must be installed.
To set a temperature compensation coefficient:
1. Install the Gilson Ethernet Utility. Refer to its documentation, if necessary.
2. Start the Gilson Ethernet Utility.
Temperature Compensation Coecient
a. To start the Gilson Ethernet Utility, click the Start button and then select
All Programs > GilsonApplications > Utilities > GilsonEthernet Utility.
b. When starting the Gilson Ethernet Utility, the Windows Firewall may block some features of the
program. Click Allow access to use the Gilson Ethernet Utility.
3. Check that the Gilson VERITY 1810 Conductivity and pH Monitor GEARS Plugins is installed
(Control Panel > Programs > Programs and Features). It is installed with TRILUTION® LC v3.0 with ServicePack 6
(or higher) and is required to communicate with the monitor.
4. Start the GEARS Application. The GEARS Application allows the Gilson Ethernet Utility to
communicate with some Gilson USB instruments, and must be running to communicate with the
VERITY® 1810 Conductivity and pH Monitor. When running, the GEARS Application appears as an
icon ( ) in the Windows system tray. To start the GEARS Application, do either of the following:
● Start TRILUTION software.
● Click the Start button, and then select AllPrograms > GilsonApplications > Utilities > GEARS > GEARSApplication.
Wait briefly for GEARS to detect the instrument.
5. In the Gilson Ethernet Utility, select VERITY 1810 Conductivity and pH Monitor from the list, and then click
Connect.
6. Select the Set Temperature Compensation command.
26
7. Enter a coefficient value for the Temperature Compensation property.
8. Click Go.
Operation | VERITY® 1810 Conductivity and pH Monitor
Page 27
Conductivity Analog Endpoint
When using the analog output to monitor conductivity, the VERITY® 1810 Conductivity and pH Monitor
includes the ability to set the analog output range, which allows adjustment of the output sensitivity.
Use the Set Analog EP command in Gilson Ethernet Utility. By default, the output range is 0–1000 mS/cm
(the full conductivity range of the monitor). The range can be reduced (0–100 mS/cm, for example) to enable
increased sensitivity for lower conductivity samples. The analog output is 0–1000 mV; therefore, by setting
the output range to 0–100 mS/cm, 1000 mV output equals 100 mS/cm, which provides the increase in
sensitivity.
To set the analog output range, use of the Gilson Ethernet Utility and the GEARS Application is required. The
Gilson VERITY 1810 Conductivity and pH Monitor GEARS Plugins must be installed.
To set the analog output range:
1. Install the Gilson Ethernet Utility. Refer to its documentation, if necessary.
2. Start the Gilson Ethernet Utility.
a. To start the Gilson Ethernet Utility, click the Start button and then select
All Programs > GilsonApplications > Utilities > GilsonEthernet Utility.
b. When starting the Gilson Ethernet Utility, the Windows Firewall may block some features of the
program. Click Allow access to use the Gilson Ethernet Utility.
Conductivity Analog Endpoint
3. Check that the Gilson VERITY 1810 Conductivity and pH Monitor GEARS Plugins is installed
(Control Panel > Programs > Programs and Features). It is installed with TRILUTION® LC v3.0 with ServicePack 6
(or higher) and is required to communicate with the monitor.
4. Start the GEARS Application. The GEARS Application allows the Gilson Ethernet Utility to
communicate with some Gilson USB instruments, and must be running to communicate with the
VERITY® 1810 Conductivity and pH Monitor. When running, the GEARS Application appears as an
icon ( ) in the Windows system tray. To start the GEARS Application, do either of the following:
● Start TRILUTION software.
● Click the Start button, and then select AllPrograms > GilsonApplications > Utilities > GEARS > GEARSApplication.
Wait briefly for GEARS to detect the instrument.
5. In the Gilson Ethernet Utility, select VERITY 1810 Conductivity and pH Monitor from the list, and then click
Connect.
6. Select the Set Analog EP command.
7. Enter a value for the Lower and Upper properties to set the range.
8. Click Go.
VERITY® 1810 Conductivity and pH Monitor | User’s Guide
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Page 29
4 MAINTENANCE
Chapter Four
When performing the maintenance described in this chapter, use good laboratory practice, including,
but not limited to, wearing protective clothing and preparing the maintenance space for service. After
completing the maintenance operation, verify the safe and good working order of the part and instrument.
This chapter contains the following information to help maintain the instrument:
● Cleaning on page 30
● Conductivity Flow Cell on page 31
You are only permitted to perform the maintenance tasks described in this user’s guide. If additional
maintenance is required, contact your local Gilson representative.
All smooth surfaces of the instrument can be cleaned with a mild, commercially available cleaning solution,
or with isopropanol on a moistened cloth.
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Cleaning
NOTICE
NOTICE
CAUTION
Cleaning
Exterior
Clean the external surfaces by applying one of the following solvents to a clean cloth and wiping.
The external surfaces of the housing may be cleaned with the following cleaning agents:
Conductivity FlowCell
Follow these instructions if you need to clean the flowcell, unclog the flowcell, replace the inlet or outlet
tubing, or replace the flowcell.
Do not maintain contact between the solvent and the surface for more than 15 seconds.
● Isopropanol
● Methanol
● Ethanol
● Sodium Hypochlorite (15% Bleach)
Be extremely careful when working with the owcell and its ttings. Flowcells are
considered expendable and are not covered by warranty if damaged or broken during any
maintenance procedure.
CLEAN THE INSIDE OF THE CONDUCTIVITY FLOWCELL
Increased noise and reduced sensitivity can be a result of a dirty flowcell.
It is often sufficient to clean the flowcell to restore optimal performance.
To prevent injury, observe good laboratory practices when handling solvents. Know the
physical and chemical properties. Refer to the Material Safety Data Sheets for the solvents
used.
To clean the inside of the flowcell:
1. Pump deionized water through the ow cell at 2–5 mL/min for 10 minutes.
2. Check monitor performance by running a sample through the system.
3. If the cleaning does not have the desired eect, repeat cleaning procedure, but substitute methanol for
deionized water.
30
Maintenance | VERITY® 1810 Conductivity and pH Monitor
Page 31
Conductivity Flow Cell
Calibration
It is recommended to calibrate the conductivity flow cell annually. Contact your local Gilson representative
for more information and assistance.
Replacement
Refer to the instructions in this section when replacing the
conductivity flowcell.
REMOVE INSTALLED FLOWCELL
1. Disconnect the USB cable from the back of the
instrument.
2. Disconnect the inlet and outlet tubing from the
flowcell.
3. Hold the installed flowcell securely with your hand,
and then lift the flowcell up and out. The flow cell is
secured with magnetic force.
Conductivity Flow Cell
INSTALL NEW FLOWCELL
1. Insert the new owcell into its holder on top of the
monitor. It will only t one way.
2. Connect the inlet and outlet tubing to the new
owcell.
Figure 13
Conductivity Flow Cell Removed
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Inlet and/or Outlet Tubing Replacement
When replacing the inlet or outlet tubing, refer to the table below for part numbers for replacement tubing
and fittings. When replacing the tubing, always replace the fittings too.
Tubing and Fittings
Flow Rate (mL/min) Part Number Description
Conductivity Flow Cell
0.5–5 49951059 TUBING,PEEK .010IDx.063OD 5FT
5–20 49952059 TUBING,.020"X 1/16 PEEK 5 FT. LONG,ORA
20–50 49953059 TUBING,.030" X 5FT, PEEK
>50 49954059 TUBING,.040" X 5FT, PEEK
All Flow Rates 49041034 FERRULE, 1/16" ANTI-TWIST, PEEK (P-250)
All Flow Rates 490410255 NUT, 1/16" ANTI TWIST PEEK (P-255)
To replace the tubing:
1. Disconnect the tubing and ttings.
1. On each end of the tubing, slide the nut,
The flattened end of the
ring should face towards
the nut
then the steel compression ring, and then
the ferrule. The attened end of the ring
should face towards the nut with the
narrow end of the ferrule towards the ring.
The narrow
end of the
ferrule is to
touch the ring
2. Insert the nut at each end into its port.
(Ports are not labeled as the direction of
the liquid flow is not specific.) Hold the
Figure 14
Plumbing Connections
tubing to the bottom of the fitting while
tightening the nut to finger tight.
32
Figure 15
Plumbing Connections (Exploded View)
Maintenance | VERITY® 1810 Conductivity and pH Monitor
Page 33
5 TROUBLESHOOTING
Chapter Five
This chapter provides information on the following topics:
● Troubleshooting on page 34
● Solvent and Buffer Compatibility on page 34
● Repair and Return Policies on page 35
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Troubleshooting
When troubleshooting a problem, first do the following:
1. Check all cabling.
Troubleshooting
2. Check all fittings.
3. Check for air in the tubing.
4. Check for leaks.
Solvent and Buer Compatibility
Chemical Resilience
All surfaces of the VERITY® 1810 Conductivity and pH Monitor housing resist limited exposure (less than
30minutes) to the following solvents and buffers, in addition to any vapors that the chemicals may emit:
● Acetonitrile
● Methanol
● Isopropyl Alcohol
● Ethanol
● Hexane
● Ethyl Acetate
● Dichloromethane
● Water
● 1M Sodium Hydroxide
● 1M Sodium Chloride
● 1M Phosphate Buffer Saline
● 1M Citrate Buffer
● 1M HEPES Buffer
● 1M Tris Buffer
● 0.1% Formic Acid
● 0.1% Triuoroacetic Acid
34
Troubleshooting | VERITY® 1810 Conductivity and pH Monitor
Page 35
Repair and Return Policies
BeforeCallingUs
Your local Gilson representative will be able to serve you more efficiently if you have the following
information:
● Serial number and model number of the instruments involved
○ The serial number is located on the underside of the instrument.
○ The serial number for the conductivity ow cell is located on the rear of the ow cell.
● Installation procedure you used
● List of concise symptoms
● List of operating procedures and conditions you were using when the problem arose
● List of other devices connected to the instrument and a description of those connections
● List of other electrical connections in the room
Warranty Repair
Repair and Return Policies
Units covered under warranty will be repaired and returned to you at no charge. If you have any questions
about applicability, please contact your local Gilson representative.
Non-Warranty Repair
For out-of-warranty repairs, contact your local Gilson representative who will discuss service options with
you and can assist in making arrangements to return the equipment, if necessary.
Return Procedure
Contact your local Gilson representative to obtain authorization before returning any Gilson equipment. To
return a piece of equipment:
● Carefully pack the unit to prevent damage in transit. Check with your local Gilson representative
regarding proper method of shipment. No responsibility is assumed by Gilson or your local Gilson
representative for damage caused by improperly packaged instruments. Indicate the authorization on
the carton and on the packing slip.
● Always insure for the replacement value of the unit.
● Include a description of symptoms, your name, address, phone number, and purchase order to cover
repair costs, return and shipping charges, if your institution requires it.
Unit End-of-Life
When a unit reaches the end of its useful life, refer to www.gilson.com for directions and
information on the end-of-life policy. This is in accordance with the European Union Directive
2002/96/EC on Waste Electrical and Electronic Equipment (WEEE).
VERITY® 1810 Conductivity and pH Monitor | User’s Guide
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Page 37
A REPLACEMENT PARTS AND ACCESSORIES
Appendix A
VERITY® 1810 Conductivity and pH Monitor
Part
Number
11100000 VERITY® 1810 Conductivity and pH Monitor with Installed Conductivity Flow Cell
Description
FlowCells
Part
Number
11100001 pH Flow Cell Assembly
11000002 Conductivity Flow Cell Assembly
Tubing and Fittings
Flow Rate (mL/min) Part Number Description
0.5–5 49951059 TUBING,PEEK .010IDx.063OD 5FT
5–20 49952059 TUBING,.020"X 1/16 PEEK 5 FT. LONG,ORA
Description
20–50 49953059 TUBING,.030" X 5FT, PEEK
>50 49954059 TUBING,.040" X 5FT, PEEK
All Flow Rates 49041034 FERRULE, 1/16" ANTI-TWIST, PEEK (P-250)
All Flow Rates 490410255 NUT, 1/16" ANTI TWIST PEEK (P-255)
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pH Sensor
Replacement Parts and A ccessories
Part
Number
11000025 pH Sensor (Replacement)
5464549103 O-Ring
Description
Cables
Part
Number
32000012 USB Cable
11000023 Analog Output Cable
Description
38
Replacement Parts and Accessories | VERITY® 1810 Conductivity and pH Monitor
Page 39
B PH FLOW CELL
Appendix B
Figure 16
VERITY® 1810 Conductivity and pH Monitor with
Optional pH Flow Cell
Technical Specifications
pH Flow Cell
Technical
Specification
pH Flow Cell
Definition
Accuracy
Maximum Drift
Part Number
Pressure
Readable Range
Specification Range
Wetted Materials
Volume
±0.1 units at 25°C
0.1/10 hours at constant conditions
11100001
0–14 pH
2–12 pH
Acrylic, PVDF, pH Glass, epoxy, BUNA-N
110 µL
psi bar MPa
100 6.89 0.69
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Setup
NOTE
NOTICE
The pH Flow Cell requires some assembly.
pH Flow Cell
There are four parts: the base, the O-ring, the pH sensor, and the securing nut.
When unpacking and storing the pH sensor, refer to the manufacturer’s instructions provided with the pH
sensor.
The O-ring provided in the soaker bottle with the pH sensor is not required for
this setup and should remain in the soaker bottle cap for future storage of the pH
sensor.
After unpacking the items, do the following:
1. Place the O-ring on the black (thinner) end of the pHsensor.
2. Insert the black end of the pH sensor with O-ring into the base.
3. Pass the end of the pH sensor cable through the securing nut.
4. Hand-tighten the securing nut to the base.
Do not use tools.
Do not overtighten.
Figure 18
Soaker Bottle
O-ring
O-ring
(part number
5464549103)
Figure 17
pH Flow Cell (Exploded View)
pH
sensor
base
securing
nut
40
pH Flow Cell | VERITY® 1810 Conductivity and pH Monitor
Page 41
Plumbing Connections
Tubing and fittings for inlet and outlet tubing connections are ordered separately so as to be appropriate for
the application flow rate. The arrows on the base indicate the flow path.
Tubing and Fittings
Flow Rate (mL/min) Part Number Description
0.5–5 49951059 TUBING,PEEK .010IDx.063OD 5FT
5–20 49952059 TUBING,.020"X 1/16 PEEK 5 FT. LONG,ORA
20–50 49953059 TUBING,.030" X 5FT, PEEK
>50 49954059 TUBING,.040" X 5FT, PEEK
All Flow Rates 49041034 FERRULE, 1/16" ANTI-TWIST, PEEK (P-250)
All Flow Rates 490410255 NUT, 1/16" ANTI TWIST PEEK (P-255)
pH Flow Cell
To make the plumbing connections:
1. On each end of the tubing, slide the
nut, then the steel compression
ring, and then the ferrule. The
attened end of the ring should face
towards the nut with the narrow
end of the ferrule towards the ring.
2. Insert the nut at each end into its
port. Hold the tubing to the bottom
of the fitting while tightening the
nut to finger tight.
The flattened end of the
ring should face towards
the nut
The narrow
end of the
ferrule is to
touch the ring
Figure 19
Plumbing Connections
outlet
inlet
Figure 20
Plumbing Connections
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Connection to the VERITY® 1810 Conductivity and pH Monitor
pH Flow Cell
Refer to the diagram when making the
pHsensor cable connection.
pH Sensor input
pH Sensor
The pH sensor cable has a BNC connector.
Connect the pH sensor cable to the pH
Sensor input, and then twist clockwise 1/4
turn to lock.
Figure 22
BNC Connector on pH Sensor Cable
Figure 21
Connection to VERITY® 1810 Conductivity and pH Monitor
Flow Path Diagram
The flow to the VERITY® 1810 Conductivity and pH Monitor should be post column and post UV detector in
an HPLC system.
to waste or
fraction collector
from
column
Figure 23
Flow Path Diagram -
VERITY® 1810 Conductivity and pH Monitor with Conductivity Flow Cell, Optional pH Flow Cell, and
159 UV-VIS Detector
42
pH Flow Cell | VERITY® 1810 Conductivity and pH Monitor
Page 43
VERITY® 1810 Monitor pH Calibration Utility
Before running samples for the first time, calibrate the pH sensor/flow cell using the
VERITY® 1810 Monitor pH Calibration Utility.
The VERITY® 1810 Monitor pH Calibration Utility is supplied on the Gilson Software USB, which is provided
with the pH flow cell.
Install the VERITY® 1810 Monitor pH Calibration Utility
PRE-INSTALLATION CHECKLIST
Before beginning the installation:
q Log on as a Windows® Administrator
q Close all running applications
q Temporarily disable antivirus software
q Temporarily disable firewall
INSTALLATION
pH Flow Cell
The installation of the VERITY® 1810 Monitor pH Calibration Utility proceeds as follows:
1. Insert the USB into the drive and then run SETUP.EXE.
2. Install the VERITY® 1810 Monitor pH Calibration Utility. Follow the on-screen instructions.
● If a User Account Control window appears, click Yes.
● If prompted with a warning about installing driver software, select to “Install this driver software
a ny w a y ”.
The installation path on a Windows® 7 (32-bit) system is:
C:\Program Files\Gilson\Utilities\VERITY 1810 Conductivity and pH Monitor\pH Calibration Utility.
The installation path on a Windows® 10 or Windows® 7 (64-bit) system is:
C:\Program Files (x86)\Gilson\Utilities\VERITY 1810 Conductivity and pH Monitor\pH Calibration Utility.
Prepare to Run the VERITY® 1810 Monitor pH Calibration Utility
1. Ensure that plumbing and electrical connections have been made as described in the Installation
chapter.
For communication to occur, the instrument must be connected via a USB connection to the PC.
2. Obtain pH 7, pH 4, and pH 10 buer solutions.
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Start the VERITY® 1810 Monitor pH Calibration Utility
NOTICE
To start the VERITY® 1810 Monitor pH Calibration Utility, click the Start button and then select
All Programs > GilsonApplications > VERITY 1810 Conductivity and pH Monitor > VERITY 1810 Monitor pH Calibration Utility.
pH Flow Cell
● If prompted to exit the GEARS Application, right-click on the icon ( ) in the Windows system tray, and
then select Exit.
● If any Windows Security Alerts appear with Gilson, Inc. as the Publisher, click Allow access.
The VERITY® 1810 Monitor pH Calibration Utility window will appear, and if communicating with the
instrument, the Serial Number and FW Version will be displayed
Use the VERITY® 1810 Monitor pH Calibration Utility
Remove the pH sensor from the pH flow cell (if installed) or
from the storage solution.
To remove the pH sensor from the pH flow cell:
1. Remove the securing nut.
2. Remove the pH sensor.
Be careful with the end of the pH
sensor. The time the end of the pH
sensor is not submerged should be
minimized.
PH 7 CALIBRATION
1. Click pH 7.
2. Use a transfer pipet to rinse the outside of
the pH sensor with deionized water and
then blot the pH sensor dry with a lint-free
cloth.
3. Use a transfer pipet to rinse the outside of
the pH sensor with pH 7 solution.
4. Submerge the pH sensor in the pH 7
solution.
5. Click OK.
6. Wait 2 minutes.
7. To repeat this calibration, click Retry or Reset.
Figure 24
VERITY 1810 Monitor pH Calibration Utility
Figure 25
On-screen Instructions for pH 7 Calibration
44
pH Flow Cell | VERITY® 1810 Conductivity and pH Monitor
Page 45
pH 4 Calibration
1. Click pH 4.
2. Wipe the outside of the pH sensor with a lint-free cloth.
3. Use a transfer pipet to rinse the outside of the pH sensor with pH 4 solution.
4. Submerge the pH sensor in the pH 4 solution.
5. Click OK.
6. Wait 2 minutes.
7. To repeat this calibration, click Retry. To repeat all calibrations, click Reset.
PH 10 CALIBRATION
1. Click pH 10.
2. Wipe the outside of the pH sensor with a lint-free cloth.
3. Use a transfer pipet to rinse the outside of the pH sensor with pH 10 solution.
4. Submerge the pH sensor in the pH 10 solution.
pH Flow Cell
5. Click OK.
6. Wait 2 minutes.
7. To repeat this calibration, click Retry. To repeat all calibrations, click Reset.
8. Use a transfer pipet to rinse the outside of the pH sensor with deionized water and then blot the
pH sensor dry with a lint-free cloth.
9. Return the pH sensor to storage solution or install in the pH ow cell (refer to the diagram on page 46,
if necessary).
Close the VERITY® 1810 Monitor pH Calibration Utility
Close the software.
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pH Flow Cell
NOTICE
Maintenance
pH Sensor Replacement
If after running the
VERITY® 1810 Monitor pH Calibration Utility,
it is determined that the pH sensor
is out of calibration, the pH sensor
(part number 11000025) and optionally,
the O-ring (part number 5464549103)
should be replaced.
O-ring
(part number
5464549103)
Refer to the instructions below and
the instructions supplied with the
replacement pH sensor.
To replace the pH sensor:
1. Disconnect the pH sensor cable from
the rear panel of the monitor by
twisting counterclockwise 1/4 turn
and then pulling.
2. Remove the securing nut.
3. Remove the pH sensor.
4. Remove the O-ring from the assembly.
Figure 26
5. Locate the new pH sensor.
6. Place the O-ring on the black (thinner)
end of the pHsensor.
7. Insert the black end of the pH sensor with O-ring into the base.
8. Pass the end of the pH sensor cable through the securing nut.
pH Flow Cell (Exploded View)
pH
sensor
base
securing
nut
46
9. Hand-tighten the securing nut to the base.
10. Connect the pH sensor cable to its port on the monitor rear panel and then twist clockwise 1/4 turn to
lock.
Do not use tools.
Do not overtighten.
pH Flow Cell | VERITY® 1810 Conductivity and pH Monitor
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