Gilson Pipetman P2G, Pipetman P200G, Pipetman P10G, Pipetman P20G, Pipetman P1000G User Manual

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Page 1

User’s Guide

Page 2

INTRODUCTION

TABLE OF CONTENTS

PIPETMAN G® | INTRODUCTION

TABLE OF CONTENTS | PIPETMAN G®

Chapter 1

INTRODUCTION

PIPETMAN G® is a fully adjustable air displacement pipette and is used with disposable tips. To answer the current needs of the loyal users of our pipette, and still conform to Gilson brand qualities, three improvements have been added to our mechanical series:

● pipetting and purge forces dramatically decreased*,

● large volumes, 5 mL and 10 mL very comfortable,

● choose a plastic ejector or a stainless steel ejector.

*Implementation of new features to improve dramatically

pipetting comfort has no impact on the legendary robustness, accuracy and precision of PIPETMAN.

Eight single channel models cover a volume range from 0.2 µL to 10 mL.

Parts Check List

Just take a moment to verify that the following items are present:

● PIPETMAN G,

● User’s Guide,

● Safety bag,

● Certiﬁcate of conformity (including barcode sticker).

Decreased pipetting forces are due to the new designed piston assembly including the use of a very high quality of lubricant. The use of other lubricant cancels the warranty of this pipette. This lubricant is available under the reference: 5440011070. Please contact your Gilson distributor.

NOTICE

TABLE OF CONTENTS

INTRODUCTION | 3

Parts CheckList | 3

GLP Features | 4

Description | 5

Speciﬁcations | 6

SETTING THE VOLUME | 8

PIPETTING | 9

Fit the Tips | 9

Pre-rinse the Tips | 10

Aspirate | 10

Dispense | 11

Guidelines for Good Pipetting | 12

ACCESSORIES | 14

TROUBLESHOOTING | 15

Leak Test | 16

MAINTENANCE | 17

CLEANING AND DECONTAMINATION | 21

SPARE PARTS | 24

WARRANTY | 26

Page 3

INTRODUCTION

PIPETMAN G® | USER’S GUIDE

INTRODUCTION | PIPETMAN G®

Description

Please refer to the following chapters for a full description of the different parts and functions of the pipette.

PIPETMAN

DIAMOND® tip

Tip ejector

Tip holder

Color-coded

push button

Blue ring

Thumbwheel

Tip ejector button

GLP Features

The Serial Number is engraved on the body of the pipette. It provides unique identification of your pipette and the date of manufacture.

Ex : AA10369

The Barcode on the box and the certiﬁcate of conformity provide traceability of your pipette.

Serial Number

Handle

Figure 2

PIPETMAN G

Figure 1

Serial Number Location

Connecting nut

Page 4

INTRODUCTION

PIPETMAN G® | USER’S GUIDE

INTRODUCTION | PIPETMAN G®

Speciﬁcations

PIPETMAN G is a high quality pipette that offers excellent accuracy and precision. The ﬁgures given in the Gilson Maximum Permissible Errors table (page 7) were obtained using PIPETMAN DIAMOND Tips. These ﬁgures are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.

Each pipette is inspected and validated by qualiﬁed technicians in accordance with the Gilson Quality System. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard, by type testing.

The adjustment is carried out under strictly deﬁned and monitored conditions (ISO 8655-6).

The data given in the tables conform to the ISO 8655-2 Standard. With a precise pipetting technique, see Guidelines for Good Pipetting on page 12, the P2G model may be used to aspirate volumes as low as

0.1 µL and the P10G model as low as 0.5 µL.

NOTICE

MAXIMUM PERMISSIBLE ERRORS

MODEL

VOLUM E (µL)

GILSON ISO 8655

SYSTEMATIC ERROR

(µL)

RANDOM ERROR (µL)

SYSTEMATIC ERROR (µL)

RANDOM ERROR (µL)

P2G

(F144054P) (F144054M)

Min. 0.2 ± 0.024 ≤0.012 ±0.08 ≤0.04

0.5

± 0.025 ≤0.012

±0.08

≤0.04

Max. 2 ± 0.030 ≤0.014 ±0.08 ≤0.04

P10G

(F144055P) (F144055M)

Min. 1 ± 0.025 ≤0.012 ± 0.12 ≤0.08

± 0.075 ≤0.030

± 0.12

≤0.08

Max. 10 ± 0.100 ≤0.040 ± 0.12 ≤0.08

P20G

(F144056P) (F144056M)

Min. 2 ± 0.10 ≤0.03 ± 0.20 ≤0.10

10 ± 0.10 ≤0.05 ± 0.20 ≤0.10

Max. 20 ± 0.20 ≤0.06 ± 0.20 ≤0.10

P100G

(F144057P) (F144057M)

Min. 10 ± 0.35 ≤0.10 ± 0.80 ≤0.30

50 ± 0.40 ≤0.12 ± 0. 80 ≤0.30

Max. 100 ± 0.80 ≤0.15 ± 0. 80 ≤0.30

P200G

(F144058P) (F144058M)

Min. 20 ± 0.50 ≤0.20 ± 1.60 ≤0.60

100 ± 0.80 ≤0.25 ± 1.60 ≤0.60

Max. 200 ± 1.60 ≤0.30 ± 1.60 ≤0.60

P1000G

(F144059P)

(F144059M)

Min. 100 ± 3 ≤0.6 ± 8 ≤3.0

500 ± 4 ≤1.0 ± 8 ≤3.0

Max. 1000 ± 8 ≤1.5 ± 8 ≤3.0

P5000G

(F144066)

Min. 500 ± 12 ≤3 ± 40 ≤15

2500 ± 15 ≤5 ± 40 ≤15

Max. 5000 ± 30 ≤8 ± 40 ≤15

P10mLG

(F1440 67)

Min. 1000 ± 30 ≤6 ± 60 ≤30

5000 ± 40 ≤10 ± 60 ≤30

Max. 10000 ± 60 ≤16 ± 60 ≤30

Gilson Maximum Permissible Errors

Each pipette model (except P5000G and P10 mLG) has two different ordering references to identify the kind of tip ejector required. For a pipette with a plastic tip ejector, the ordering reference is ended by the letter P, for a pipette with a stainless steel tip ejector, the ordering reference is ended by the letter M. Ex: For a P10G model with the plastic tip ejector the ordering reference is F144055P. For the same pipette with a stainless steel tip ejector, the ordering reference is F144055P.

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PIPETTING

SETTING THE VOLUME

SETTING THE VOLUME | PIPETMAN G®

Chapter 3

PIPETTING

It is recommended to use PIPETMAN® DIAMOND Tips with the PIPETMAN G for optimum performance. These tips are made from pure polypropylene. Plastic tips are for a single application—they must not be cleaned for reuse.

Fit the Tips

To fit a new PIPETMAN DIAMOND Tip, push the tip holder into the tip using a slight twisting motion to ensure a ﬁrm, airtight seal.

For the P2G and P10G Models Equipped with Stainless Steel Tip Ejector,

A dual-position adapter (plastic) is required to fit DL10 tips (long tips) or D10 tips (short ti ps).

P2G and P10G models are delivered with the adapter in place, ready to use DL10 tips. If D10 tips are used, the adapter must be repositioned in the shorter slot as follows:

1. Pull the adapter down from the metallic rod.

2. Turn the adapter through 180°C.

3. Reﬁt the adapter so that the end of the metallic rod engages the shorter slot of the adapter.

Short tips

Long tips

Figure 5

Dual-Position Adapter for P2G and P10G with stainless steel tip ejector

Chapter 2

SETTING THE VOLUME

The volume of liquid to be aspirated is set using the volumeter. The dials are colored either black or red to indicate the position of the decimal point, depending on the model (see examples).

The volume is set by turning the thumbwheel or the push button. The push button makes it easier and quicker to set volumes, especially when wearing gloves. The thumbwheel may be turned using only one hand to slowly reach the required setting.

To obtain maximum accuracy when setting the volume, proceed as follows:

● when decreasing the volume setting, slowly reach the required setting, making sure not to overshoot the mark.

● when increasing the volume setting, pass the required value by 1/3 of a turn and then slowly decrease to reach the volume, making sure not to overshoot the mark.

MODEL

COLOR OF VOLUMETER NUMBERS

BLAC K

RED

INCREMENT

P2G µL 0.01 µL 0.002 µL

P10G to P20G µL 0.1 µL 0.02 µL

P100GP200G µL - 0. 2 µL

P1000G 0.01 mL mL 0.002 m L

P5000G 0 .01 mL

mL 0.002 mL

P10mLG mL 0.01 m L 0.02 mL

P2G

1.25 µL

0 7

P10G

7.5 µL

1 2

P20G

12.5 µL

P100G

0 7 5

75 µL

P200G

1 2 5

125 µL

P1000G

7 5

0.75 mL

P5000G

2 5

1.25 mL

P10mlG

0 7

7.5 mL

Push button

Thumbwheel

Figure 3

Dial Colors by Model

Figure 4

Location of Push Button and Thumbwheel

PIPETMAN G® | USER’S GUIDE

Page 6

PIPETTING

PIPETMAN G® | USER’S GUIDE

PIPETTING | PIPETMAN G®

1 2

First stop Second

stop

Dispense

1. Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).

2. Press the push button slowly and smoothly to the first stop.

3. Wait for at least a second, then press the push button to the second stop to expel any residual liquid from the tip. Keep the push button pressed fully down and (while removing the pipette) draw the tip along the inside surface of the vessel.

4. Release the push button, smoothly. Eject the tip by pressing firmly on the tip ejector button.

Figure 6

Pipetting Motion - Aspirate and Dispense

For the P2G and P10G Models Equipped with Plastic Tip Ejector,

A tip ejector extension is supplied to fit with D10 tips (short tips).

To fit a tip ejector extension:

1. Slide the extension over the tip holder.

2. Push the extension firmly onto the end of the tip ejector until it clicks into place.

To remove a tip ejector extension:

1. Gently twist the extension.

2. Pull it away from the pipette.

Both dual-position adapter and tip ejector extension are autoclavable .

Pre-rinse the Tips

Some liquids (e.g.,protein-containing solutions and organic solvents) can leave a film of liquid on the inside wall of the tip; pre-rinse the tip to minimize any errors that may be related to this phenomenon.

Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel (or to waste). Subsequent volumes that you pipette will have levels of accuracy and precision within specifications.

Aspirate

1. Press the push button to the ﬁrst stop (this corresponds to the set volume of liquid).

2. Hold the pipette vertically and immerse the tip in the liquid (see immersion depth table, page 12). Release the push button slowly and smoothly (to top position) to aspirate the set volume of liquid. Wait one second (time depends on model, see table); then withdraw the pipette-tip from the liquid. You may wipe any droplets away from the outside of the tip using a medical wipe, however if you do so take care to avoid touching the tip’s oriﬁce.

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PIPETTING

PIPETMAN G® | USER’S GUIDE

PIPETTING

PIPETMAN G® | USER’S GUIDE

PIPETTING | PIPETMAN G®

10. You may remove the tip ejector, see

CHAPTER 6 - MAINTENANCE to aspirate

from very narrow tubes.

11. After pipetting acids or other corrosive liquids that emit vapors, remove the tip ejector, the tip holder, rinse, dry and lubricate the piston, see

CHAPTER 6 - MAINTENANCE. For the model

P1000G, by using a specific tip holder equipped with a filter, you can increase the lifetime of the piston, see CHAPTER 4 - ACCESSORIES.

12. Do not pipette liquids having temperatures above 70°C or below 4°C. The pipette can be used between + 4°C and + 40°C but the speciﬁcations may vary according to the temperature (refer to the ISO 8655-2 standard for conditions of use).

Guidelines for Good Pipetting

1. Make sure that you operate the push button slowly and smoothly.

2. When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table).

3. Change the tip before aspirating a different liquid, sample, or reagent.

4. Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.

5. Each new tip should be pre-rinsed with the liquid to be pipetted.

6. Liquid should never enter the tip holder; to prevent this:

● press and release the push button slowly and smoothly,

● never turn the pipette upside down,

● never lay the pipette on its side when there is liquid in the tip.

7. If you use the same tip with a higher volume, pre-rinse the tip.

8. For volatile solvents you should saturate the air-cushion of your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample.

9. When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip several times before use.

TABLE - IMMERSION DEPTH AND WAIT TIME

MODEL

IMMERSION DEPTH (MM)

WAIT TIME (SECONDS)

P2G 1 1

P10G 1 1

P20G 2-3 1

P100G 2-4 1

P200G 2-4

P1000G 2-4

2-3

P5000G 3-6

4-5

P10mLG 5 -7 4-5

Page 8

Chapter 5

TROUBLESHOOTING

A quick inspection of the pipette may help you to detect a problem.

The following table may help you to identify and correct the problem you might encounter.

TROUBLESHOOTING

ACCESSORIES

PIPETMAN G® | USER’S GUIDE

ACCESSORIES | PIPETMAN G®

SYM PTOM POSSIBLE CAUSE ACTION

Pipette i s leaking sample

Damaged tip holder Worn O-r ing or seal

Replac e the tip hol der Replac e both par ts and lubrica te

Pipette w on’t asp irate

Worn O-ring Damaged tip holder Connecting nut is loose Damag ed or corroded pi ston Improper repair or assembly

Replac e both parts an d lubricate Replac e the tip holder Tighten connecting nut Return pi pette to supplie r See CHAPTER 6 - MAINTENANCE

Pipette i s inaccurate

Improper repair or assembly Unscrewe d tip holder Connecting nut is loose

See CHA PTER 6 -

MAINTENANCE

Tighten connecting nut Tighten connecting nut

Pipette i s not precise

Tip hold er is loose Connecting nut is loose Incorrect operator technique Damag ed or corroded pi ston(s) Damaged tip holder(s) Worn O-r ing or seal

Tighten connecting nut Tighten connecting nut Operator training Return pi pette to supplie r Replac e the tip holder Replace both parts and lubrica te

Tips fal l o or do not ﬁt co rrectly

Low quali ty tips Dirty t ip holde r Damaged tip holder(s) Damaged tip ejector Ejector s pacer is missing The ejec tor spacer is dam aged The tip ej ector is lo ose The ejec tor lock is misal igned

Use PIP ETMAN D IAMOND tips Clean th e tip holder with al cohol Replac e the tip holder Replac e tip ejec tor Mount th e spacer on the ti p ejector Replac e the ejec tor space r Assem ble the tip ejec tor properly Align the e jector lock

Pipett ing seize up Pi ston nee d lubric ant Lubricate piston assembly

If you cannot solve the problem, contact your local Gilson representative.

NOTE

You may download from the Gilson website (www.gilson.com) the “2 minute inspection poster”, which shows how to perform a quick diagnosis of your pipette.

Chapter 4

ACCESSORIES

To make pipetting more comfortable and more secure, Gilson has developed several accessories:

Pipette stands allow users to store pipettes vertically to avoid the possibility of liquid running back into the pipette.

DESCRIPTION P/N

CARROUSEL™ pipette stand (7 pipettes) F161401

TRIO™ stand (3 pipettes) F161405

SINGLE™ PIPETTE HOLDER F161406

To identify or personalize your pipette, COLORIS™ clips are available:

DESCRIPTION P/N

COLORIS™ clips (mixed colors set of 10) F161301

COLORIS clips (red, set of 10) F161302

COLORIS clips (yellow, set of 10) F161303

COLORIS clips (green, set of 10) F161304

COLORIS clips (blue, set of 10) F161305

COLORIS CLIPS (WHITE, SET OF 10) F161306

With The JIMMY™, a hands-free microtube opener, you can open both snap-cap and screw-cap microtubes.

DESCRIPTION P/N

The Jimmy™ (set of 3) F144983

To protect the piston when pipetting corrosive liquids, you can use a specific tip holder and filter for the model P1000N:

DESCRIPTION P/N

CORROSION PROTECTION KIT (TIP HOLDER + A BAG OF 10 FILTERS) F144570

Page 9

MAINTENANCE

PIPETMAN G® | USER’S GUIDE

MAINTENANCE

TROUBLESHOOTING

TROUBLESHOOTING | PIPETMAN G®

Chapter 6

MAINTENANCE

Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance.

Maintenance is limited to:

● Cleaning or decontamination, see CHAPTER 7 -

CLEANING AND DECONTAMINATION.

● Replacing spare parts

● Greasing the piston assembly.

PIPETMAN P2G and P10G should not be disassembled, so you may only replace the push

button, tip ejector, dual position tip ejector and its adapter. With these pipettes if the tip holder is damaged, the piston may also be damaged.

After replacing any parts you should verify the performance of your pipette following the veriﬁcation procedure available on the Gilson website (www.gilson.com). If the pipette needs to be readjusted, please contact your local Gilson Service Center.

Changing the Tip Ejector

To remove

Push the ejection button.

Push laterally the tip ejector.

Slide and remove the tip ejector.

To r e f i t

Push the ejection button.

Slide the tip ejector along the tip holder.

Clip the tip ejector on the body of the pipette.

NOTICE

Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of chemical, biological, or radioactive contamination. Refer to CHAPTER 7 - CLEANING AND

DECONTAMINATION. Please use

the included safety bag to return the pipette to your local Gilson Service Center.

CAUTION

Leak Test

This test may be performed at any time to check that the pipette does not leak, especially after performing a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test.

For the P2G to P200G Models:

1. Fit a PIPETMAN DIAMOND Tip.

2. Set the pipette to the maximum volume given in the speciﬁcations, and pre-rinse.

3. Aspirate the set volume from a beaker of distilled water.

4. Maintain the pipette in the vertical position and wait for 20 seconds.

5. If a water droplet appears at the end of the tip there is a leak.

6. If you see no droplet, re-immerse the tip below the surface of water.

7. The water level inside the tip should remain constant; if the level goes down there is a leak.

For the P1000G, P5000G, and P10mLG Models:

1. Fit a PIPETMAN DIAMOND Tip.

2. Set the pipette to the maximum volume given in the speciﬁcations.

3. Aspirate the set volume from a beaker of distilled water.

4. Maintain the pipette in the vertical position and wait for 20 seconds.

5. If a water droplet appears at the end of the tip, there is a leak.

Page 10

MAINTENANCE

PIPETMAN G® | USER’S GUIDE

MAINTENANCE | PIPETMAN G®

Changing the Tip Holder – No Tools Required

1. Remove the tip ejector (see page 17).

2. Unscrew the connecting nut by turning it counter-clockwise.

3. Carefully separate the lower and upper parts.

4. Remove the piston assembly and the seals.

5. Clean, autoclave, or replace the tip holder.

6. If necessary, lubricate lightly the piston and its seals (see below).

7. Reassemble the pipette (refer to the ﬁgure, p 18).

8. Tighten the connecting nut (turn clockwise)

9. Reﬁt the tip ejector (see page 17).

Servicing the Piston Assembly

You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the pipette must be adjusted and calibrated in a local Gilson Service Center. As the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself.

1. Remove the tip ejector (see page 17).

2. Unscrew the connecting nut by turning it counter-clockwise.

3. Carefully separate the lower and upper parts.

4. Remove the piston assembly and the seals.

5. Leave exposed the piston, clean it with isopropanol or ethanol and lubricate lightly.

For P100G, P200G, and P1000G models, lubricate

only the useful part of the piston and the seals. For P5000G and P10 mLG, lubricate the tip of the piston, disassemble the seals and lubricate their internal part.

The piston assembly must not be autoclaved.

NOTICE

P10G, P20G

P100G, P200G

P5000G P10mLG

P1000G

Tip holder

Tip ejector

Stainless Steel

Plastic

Connecting nut

Color coded push button

Piston

assembly

Blue ring

Figure 7

Piston Assembly (Disassembled)

Page 11

CLEANING AND DECONTAMINATION

MAINTENANCE

PIPETMAN G® | USER’S GUIDE

MAINTENANCE | PIPETMAN G®

Chapter 7

CLEANING AND

DECONTAMINATION

PIPETMAN G is designed so that the parts normally in contact with liquid contaminants, can easily be cleaned and decontaminated. However, because the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself; please contact your local Gilson Service Center.

Cleaning

The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is recommended for cleaning PIPETMAN G.

External

1. Remove the tip ejector (see page 17).

2. Wipe the tip ejector with a soft-cloth or lint-free tissue impregnated with soap solution.

3. Wipe the entire pipette with a soft-cloth or lint-free tissue impregnated with soap solution, to remove all dirty marks. If the pipette is very dirty, a brush with soft plastic bristles may be used.

4. Wipe the entire pipette and the tip ejector with a soft cloth or lint-free tissue soaked with distilled water.

5. Reﬁt the tip ejector and allow the pipette to dry.

You may refer to the decontamination procedure available on the Gilson website (www.gilson.com). Liquid must

never enter the upper part (handle) of any pipette.

NOTICE

The use of other lubricant cancels the warranty of this pipette.

NOTICE

6. Reassemble the pipette (refer to ﬁgure 7, page 18).

7. Tighten the connecting nut (turn clockwise).

8. Reﬁt the tip ejector (see page 17).

Changing the Seals

The O-ring and seal are on the piston; they must not be autoclaved, if worn or damaged in any

way (chemical or mechanical), they must be replaced. As the models P2G and P10G contain miniaturized parts, it is best not to disassemble these pipettes yourself, please contact your local Gilson Service Center.

The dimensions of the O-ring vary depending on the model of pipette.

1. Remove the tip ejector (see page 17).

2. Unscrew the connecting nut by turning it counter-clockwise.

3. Carefully separate the lower and upper parts.

4. Remove the piston assembly, O-ring and seal.

5. If necessary clean the piston and replace the seal; lubricate them lightly. Please place them in the correct order.

6. Reassemble the pipette (refer to the ﬁgure 7, page 18).

7. Tighten the connecting nut (turn clockwise).

8. Reﬁt the tip ejector (see page 17).

Page 12

CLEANING AND DECONTAMINATION

PIPETMAN NEO® | USER’S GUIDE

CLEANING AND DECONTAMINATION | PIPETMAN NEO®

Chemical Decontamination

You may choose to decontaminate your pipette chemically, in accordance with your own procedures. Whatever decontaminant you use, check with the supplier of the decontaminant that it is compatible with stainless steel and the plastics used in the construction of the pipette: PA (Polyamide), PBT (Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephtalate), POM (Polyoxymethylene), or PVDF (Polyvinylidene Fluoride).

Upper Part (handle)

1. Wipe the upper part (handle) of the pipette with a soft-cloth or lint-free tissue impregnated with the chosen decontaminant.

2. Wipe the upper part of the pipette with a soft-cloth or lint-free tissue soaked with distilled water or sterile water.

Lower Part (volumetric module)

The following components only can be immersed in a decontaminant solution: connecting nut, tip ejector, tip holder.

Piston assembly and seals must be degreased with isopropanol or ethanol before being immersed in decontamination solution in separate vessel.

1. Remove the tip ejector, the tip holder and the connecting nut.

2. Immerse them in the cleaning solution.

3. Degrease the piston assembly, the seals and immerse them in another vessel.

4. Rinse each component with distilled water.

5. Leave the parts to dry by evaporation (or wipe with a soft cloth the tip ejector, the tip holder and connecting nut).

6. Lubricate the piston assembly and the seals.

7. Reassemble the piston assembly, the tip holder and the tip ejector.

Internal

The following components only can be immersed in a cleaning solution: connecting nut, tip ejector, tip holder, piston assembly, seal and O-ring.

1. Disassemble the pipette as described in the

CHAPTER 6 - MAINTENANCE.

2. Set aside the upper part in a clean, dry place.

3. Clean the individual components of the lower part of the pipette using an ultrasonic bath (20 minutes at 50°C) or with a soft-cloth and brushes. Note that the piston assembly and seals must be degreased with isopropanol or ethanol before being immersed in the ultra sonic bath. Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder.

4. Rinse the individual components with distilled wate r.

5. Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue.

6. Reassemble the pipette as described in the

CHAPTER 6 - MAINTENANCE.

Autoclaving

The upper part (body) and the piston assembly of the pipette are not autoclavable. Only the following parts may be autoclaved: tip ejector, tip holder and connecting nut. The O-ring and seal are not autoclavable; they may be cleaned or replaced with the one speciﬁed in CHAPTER 8 - SPARE

PARTS.

1. Clean the parts to be autoclaved, especially the tip holder.

2. Put the parts in an autoclaving sack.

3. Autoclave for 20 minutes at 121°C, 0.1 MPa.

4. Check that the parts are dry before re-assembling the pipette.

5. Set the pipette aside to stabilize at room temperature.

6. Reassemble the pipette as described in the

CHAPTER 6 - MAINTENANCE.

Page 13

SPARE PARTS

PIPETMAN G® | USER’S GUIDE

SPARE PARTS | PIPETMAN G®

Chapter 8

SPARE PARTS

Service Kit 1

level includes:

● 3 piston seals or seal guides

● 3 O-rings

● 1 tip holder

Service Kit 2nd level includes:

● 1 push button

● 1 connecting nut

● 2 tip ejectors

for the P2G and P10G models only:

● 1 adapter for stainless steel tip ejector

● 1 extension for plastic tip ejector

P2G (F 144054P OR M) AND P10G (F14 4055P OR M)*

DESCRIPTION

P/N F OR P2G

P/N F OR P10G

C+D+E Service Kit 1st level F144501 FA070 01

A+B+F1 to F4 Servi ce Kit 2nd level F1619702 F1619712

C+D Seal + O-ring ( 5 se ts) F 144861 FA 07012

F3 Tip ejector adap ter F 144879 F144879

F4 Tip ejector extension F2070 903

F2070903

P20G (F144056P OR M) AND P10 0G (F 144057P OR M)*

DESCRIPTION

P/N F OR P20G

P/N F OR P100G

C+D+E Service Kit 1st level FA070 02 FA070 03

A+B+F Ser vice Kit 2nd level F1 61972 2 F1619732

C+D Seal + O-ring ( 5 se ts) FA 07013 FA07014

P200G (F144 058P OR M) AND P100 0G (F 144059P OR M)*

DESCRIPTION

P/N F OR P200G

P/N F OR P1000G

C+D+E Service Kit 1st level FA070 04 FA070 05

A+B+F Ser vice Kit 2nd level F 161 9742 F 1619752

C+D Seal + O-ring ( 5 se ts) FA 0701 5 FA07016

P5000G (F14 4066) AN D P10MLG (F14 4067)*

DESCRIPTION

P/N F OR P5000G

P/N F OR P10MLG

C+D+E Service Kit 1st level FA070 21 FA0702 2

A Service Kit 2nd level FA0701 9 FA0 702 0

C+D Seal + O-ring ( 5 se ts) FA 0701 7 FA0701 8

E Tip holder F123608 F 161263

Each pipette model (except P5000G and P10mLG) has two different ordering references to identify the kind of tip ejector required. For a pipette with a plastic tip ejector, the ordering reference is ended by the letter P, for a pipette with a stainless steel tip ejector, the ordering reference is ended by the letter M. Ex: For a P10G model with the plastic tip ejector the ordering reference is F144055P. For the same pipette with a stainless steel tip ejector, the ordering reference is F144055M.

DESCRIPTION PART NUMBER

Lubricant* 5440011 070

Figure 8

Spare Parts Identified

Page 14

NOTES

WARRANTY

PIPETMAN G® | USER’S GUIDE

WARRANTY | PIPETMAN G®

Chapter 9

WARRANTY

Gilson warrants this pipette against defects in material under normal use and service for a period of 12 months from the date of purchase. This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or inadequate maintenance (contrary to the recommendations given in the User’s Guide), including, but not limited to pipettes which have been subjected to physical damage, improper handling, or spillage or exposure to any corrosive environment. This warranty shall also be void in the event pipettes are altered or modiﬁed by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes.

THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.

NOTES

Page 15

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3000 Parmenter Street • PO Box 620027 Middleton, WI 53562 USA T: 608-836-1551 or 800-445-7661 • F 608-831-4451

GilsonS.A.S.

19, avenue des Entrepreneurs BP 145 • F-95400 Villiers-le-Bel, France T +33 (0) 1 34 29 50 00 • F +33 (0) 1 34 29 50 20

Gilson Pipetman P2G, Pipetman P200G, Pipetman P10G, Pipetman P20G, Pipetman P1000G User Manual

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