9
Release the push-button, smoothly. Eject the tip by
pressing rmly on the tip-ejector button.
6 GENERAL GUIDELINES FOR GOOD PIPETTING
Make sure that you operate the push-button
slowly and smoothly.
When aspirating, keep
the tip at a constant
depth below the surface of the liquid (refer
to the table).
Change the tip before
aspirating a dierent
liquid, sample, or reagent.
Change the tip if a droplet remains at the end of the
tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid
to be pipetted.
Liquid should never enter the tip-holder; to prevent
this:
- press and release the push-button slowly and
smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is liquid
in the tip.
If you use the same tip with a higher volume, prerinse the tip.
For volatile solvents you should saturate the aircushion of your pipette by aspirating and dispensing
the solvent repeatedly before aspirating the sample.
When the temperature of the liquid is dierent from
the ambient temperature, pre-rinse the tip several
times before use.
You may remove the tip-ejector (see Chapter 11 -
Maintenance) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids
that emit vapors, remove the tip-holder, rinse the
Table - Immersion Depth
and Wait Time
Model Immersion Wait Time
Depth (mm) (seconds)
P2N 1 1
P10N 1 1
P20N 2-3 1
P100N 2-4 1
P200N 2-4 1
P1000N 2-4 2-3
8x20, 12x20 2-3 1
8x200, 12x200 2-3 1