Gilson PIPETMAN M P300M, PIPETMAN M P100M, PIPETMAN M P20M, PIPETMAN M P200M, PIPETMAN M P1200M User Manual

User’s Guide

EN

M

connected

M

TABLE OF CONTENTS

TABLE OF CONTENTS | PIPETMAN® M CONNECTED

TABLE OF CONTENTS

INTRODUCTION | 4

PARTS CHECKLIST AND ACCESSORIES | 5

Parts Checklist | 5
Accessories | 5

GETTING STARTED | 5

DESCRIPTION | 6

Reset | 6
Switch O | 6
Display | 6

PIPETTING JUST LIKE WITH PIPETMAN | 7

Switch On | 7
Adjust The Volume Setting | 7
Purge Volume | 7

PIPETTING MODES | 7

PIPET Mode | 7
REPETITIVE Mode | 8
MIX Mode | 9
REVERSE Mode | 11
CUSTOM Mode | 12

BENEFIT FROM THE BLUETOOTH CONNECTION | 13

Connect via Bluetooth | 13
Connection with the PIPETMAN M Software | 14

PIPETTING SPEED CONTROL | 15

PIPETMAN® DIAMOND TIPS | 15

GUIDELINES FOR GOOD PIPETTING | 17

Aspirate and Dispense | 17
General Guidelines for Good Pipetting | 18

PERSONALIZE YOUR PIPETMAN® M CONNECTED | 18

Tip Ejector Extension for Use with P10M | 18
Personal Label – Name and/or Application Tag | 19

GOOD LABORATORY PRACTICE FEATURES | 19

General | 19
Cycle Counters | 19
Alarms | 20

TABLE OF CONTENTS

PIPETMAN® M CONNECTED | USER’S GUIDE

POWER MANAGEMENT | 20

Battery Charging | 20
Working with Charger Connected to the Pipette | 21
Low Battery Warning | 21
Changing the Battery | 21

CONFIGURATION | 22

MAINTENANCE | 22

Service Information | 23
Adjustment | 24
Standard Readjustment | 24
Maintenance Operations | 24
Maintenance Warning | 25
Maintenance Operation for Single Channel Models | 25
Maintenance Operation for Multichannel Models | 27

CLEANING AND DECONTAMINATION | 28

Cleaning | 28
Single Channel Models | 28
Multichannel Models | 29
Decontamination | 29

Chemical Decontamination – Single and Multichannel Models | 29

LEAK TEST | 30

TROUBLESHOOTING | 31

SPECIFICATIONS | 31

SAFETY PRECAUTIONS AND LIMITATIONS OF USE | 33

REPLACEMENT PARTS | 34

Single Channel Models | 34
Multichannel Models | 34

APPENDICES | 35

Appendix A – Example of a Performance Check | 35
Appendix B – Z Factor | 36
Appendix C – Evaporation Loss | 37

REGULATORY COMPLIANCE | 38

WARRANTY | 39

INTRODUCTION

4

INTRODUCTION | PIPETMAN® M CONNECTED

Chapter 1

INTRODUCTION

Congratulations on the purchase of your PIPETMAN® M Connected, the electronic
pipette with guaranteed performance in both standard and repetitive pipetting
modes. For more information visit www.gilson.com/en/guaranteedperformance.

PIPETMAN M Connected is a Bluetooth® enabled motorized member of the
PIPETMAN® family of pipettes from Gilson, requiring virtually no pipetting forces to
aspirate and dispense samples. Combining PIPETMAN’s renowned accuracy, precision,
and robustness with user-friendly functions, PIPETMAN M Connected will reduce
pipetting fatigue, and increase pipetting efficiency.

PIPETMAN M Connected is a unique pipette with only two
buttons to operate the pipette and reach all menu options.

PIPETMAN M Connected features the classic PIPETMAN look
with additional functionality:

● Intuitive interface with five pipetting modes for a large
number of applications: forward pipetting, repetitive, mix,
reverse, and custom mode.

● Minimal effort: aspirate and dispense with one click of the
push button and eject tips with ease to help reduce the
risk of repetitive strain injuries (RSI).

● Maximum comfort: the lightweight and balanced design of PIPETMAN M Connected
offers an ergonomic design that rests comfortably in your hand as you pipette.

● Minimized pipetting variability: the motorized piston allows for high reproducibility
and accuracy while pipetting.

● Increased performance for all day pipetting comfort.

PIPETMAN M Connected is available in 20 models covering a range from 0.5 µL to 10 mL
in single channel and 0.5 µL to 1200 µL in multichannel:

Menu
button

Push
button

PIPETMAN M CONNECTED SINGLE CHANNEL

Model

Part Number

Volume Range

P10M F81040 0.5–10 µL

P20M F81041 2–20 µL

P100M F81042 5–100 µL

P200M F81043 20–200 µL

P300M F81044 20–300 µL

P1200M F81045 100–1200 µL

P5000M F81046 500–5000 µL

P10mLM F81047 1–10 mL

Chapter 1

PIPETMAN M CONNECTED MULTICHANNEL

Model Part Number

Volume Range

P8x10M F81048

0.5–10 µL

P12x10M F81049

P8x20M F81050

1–20 µL

P12x20M F81051

P8x100M F81052

10–100 µL

P12x100M F81053

P8x200M F81054

20–200 µL

P12x200M F81055

P8x300M F81056

10–300 µL

P12x300M F81057

P8x1200M F81058

50–1200 µL

P12x1200M F81059

PARTS CHECKLIST AND ACCESSORIES

5

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 2

PARTS CHECKLIST AND ACCESSORIES

Parts Checklist

Take a moment to verify that the following items are in the box:

• PIPETMAN M Connected pipette

• Power Supply 5V with AC adapter and cable

• 4 Battery side tags

• 4 Ejector side tags

• Tip ejector extension (P10M model only)

• Quick Guide

Accessories

ACCESSORIES PART NUMBER

POWER CARROUSEL: 5–position charging carrousel for single and
multichannel models FB1001

Battery side tags (set of 4) F807013

Ejector side tags (set of 4) F807014

USB Dongle F807027

Chapter 3

GETTING STARTED

Switch on your PIPETMAN M Connected. Press the push button to activate
the pipette. The start-up screen appears:

The pipette will do a self calibration test and the firmware
will reset the piston.

During initialization, the volume range, version of the
firmware, and the number of readjustments is displayed on
the screen (refer to Standard Readjustment on page 24).

Your PIPETMAN M Connected is preset on PIPET
mode and is now ready to pipette (Refer to Chapter 5

Pipetting Just Like with PIPETMAN on page 7).

PIPETMAN M Connected goes into sleep mode after three minutes of inactivity.
The display will shut off. Just press the push button to reactivate the pipette. Your last
setting and pipetting mode will appear on the screen.

• Safety bag

• Certificate of conformity
(including bar-code sticker

• Lubricant (except for P10M, and
the multichannel models)

Volume
range

Version of
firmware

Number of
readjustments

Please contact your Gilson representative to order additionnal accessories.

NOTE

PIPETMAN M Connected is provided with a minimal battery charge. Before
using your new pipette, we strongly recommend that you to fully charge the
battery. PIPETMAN M Connected charges 80% of its full battery capacity in
less than an hour, but it takes three hours to fully charge the battery. Please
refer to Chapter 12 Power Management on page 20 for charging procedure.

DESCRIPTION

6

DESCRIPTION | PIPETMAN® M CONNECTED

Figure 1

PIPETMAN® M Connected single and multichannel models

Chapter 4

DESCRIPTION

1

2

3

4

5
6

7

8

9

10

11

12

13

14

15

16

17

18

19

Upper part

Push button and volume
adjustment knob

Menu button: direct access to all
menu functions

Tip ejector button
Display
Elastomer plug to cover the battery

charging port
Identity-tag clip
Identity-tag window
Connecting nut — attaches handle

to lower part

Lower part

Tip ejector: removable to access tip
holder

Optimized tip holder to reduce tip
fitting and ejection forces, removable
for cleaning and servicing

Ejector clip
Ejector support
Cover

Ejector spacer

Screen

Pipetting Mode
Aspirate and dispense indicator

Battery status
Purge indicator

Volume

1

2

3

4

5

6

7
8

9

10

11
12
13
14

15
16
17
18
19

Reset

To reset your pipette, simultaneously press on the push and menu button for at
least five seconds.

Switch O

To switch off your PIPETMAN M Connected, press the push button for at least five
seconds.

Display

The PIPETMAN M display is an Organic Light-Emitting Diod (OLED) screen. It shows
the current mode and operation step, pipetting volume, battery indicator and piston
status (aspirated and dispensed volume) in real time.
Warning messages appear in the place of volume.

1

2

1

PIPETTING JUST LIKE WITH PIPETMAN

7

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 6

PIPETTING MODES

PIPETMAN M Connected offers more pipette modes for a large number of applications:
you will find all pipetting modes in the menu (for system settings refer to Chapter 8

Pipetting Speed Control on page 15 and Chapter 14 Configuration on page 22). Access

the menu by pushing the menu button. To choose one of the pipetting modes, turn
the push button and then click to confirm your selection.

PIPET Mode

This is the classic pipette mode (forward pipetting) for simple
aspirating and dispensing.

•

Chapter 6

Chapter 5

PIPETTING JUST LIKE WITH PIPETMAN

Using PIPETMAN M Connected is as easy as using a mechanical
PIPETMAN. Your PIPETMAN M Connected is set by default to

“PIPET

Mode”,

which means forward pipetting. Select a volume and then start

to pipette.

Switch On

Press push button.

Adjust The Volume Setting

1. Hold your PIPETMAN M Connected in a nearly vertical position.

2. Turn the push button half way. The screen will display:
“

Click to change volume.

”

3. Press the push button; the volume on the display will start blinking,
you can now adjust the volume:

● Turn the push button either clockwise to decrease volume or counter
clockwise to increase volume, as indicated on the button.

● Press the push button one time when finished. The volume is locked.

Chapter 5

NOTE

You can use the PIPET Mode for all standard applications like DNA
extraction, plasmid isolation, cloning, dilution, PCR, qPCR and many others.
You can easily pipette aqueous liquids like buer, chemical solutions
(MgCl2, KCl …), and biological samples such as blood, DNA, and RNA.

Purge Volume

The tip can be emptied at any time during a pipetting cycle.

1. Turn the push button quickly: the message

“Click to abort”

will appear on the

screen.

2. Click on the push button to validate: the liquid will be dispensed and an automatic
purge followed by piston reset to zero will occur.

PIPETTING MODES

8

PIPETTING MODES | PIPETMAN ® M CONNECTED

Fit a tip suitable for the model of PIPETMAN M Connected that you are using
(preferably use PIPETMAN Tips for assurance of accurate and precise results, refer to
Chapter 9 PIPETMAN DIAMOND Tips on page 15).

1. Press the push button to aspirate the selected volume.

2. To dispense: click (press and release) the push button.
Three things happen: sample dispense, automatic
purge, and piston reset to zero. Or, keep your thumb
pressing the push button until the end of the dispense
cycle: the piston reset takes place after releasing the
push button again. This feature enables you to remove
the tip from the liquid, without aspirating anything.

3. Eject tip by pressing the ejection button.

Your PIPETMAN M Connected is ready for the next pipetting
cycle while maintaining the volume and the last settings.

Continue pipetting as you would with your mechanical PIPETMAN Connected; it’s as
simple as that to use your new, electronic pipette!

REPETITIVE Mode

The REPETITIVE Mode allows you to distribute the same volume repeatedly in a
predefined number (N) of equal aliquots. You supply the aliquot volume
(AV) and the pipette automatically calculates the number of aliquots
possible from the nominal (maximum) volume (NV) of the pipette as
follows:

N = NV/AV (e.g., 120 μL x 10 for P1200M).

You also can decrease the number of repetitions (e.g., 120 μL x 10-(n ≥1) for P1200M).

NOTICE

Always fit a tip before using any pipette.

START button
Optional STOP

0

V

Purge

Figure 2

PIPET Mode

NOTE

The REPETITIVE Mode is ideal for dispensing aliquots, e.g., dispensing a
PCR master mix into PCR tubes or 96-well, pipetting elution buer for DNA
extraction, preparing a target for spectrometric analyses, distributing loading
buer into samples, etc.

1. Press menu button to access system menu, where you can choose between
different pipette modes. Select REPETITIVE Mode by turning the push button.
To confirm your selection click on the push button.

2. Turn the push button half way, the screen will display:

“Click to change volume.”

3. Press the push button; the volume on the display will start to blink,
you can now adjust the volume.

Set the aliquot volume

1. Cick the push button, set the volume by turning the push button and click again
to confirm your settings.

2. Set the aliquot number: the maximal number of aliquots has been calculated
by your PIPETMAN M Connected. The number of aliquots flashes and you can
decrease this number (N – (n ≥1)) by turning the push button.

3. Press again to confirm your settings.

PIPETTING MODES

9

PIPETMAN® M CONNECTED | USER’S GUIDE

4. Fit a tip suitable for the model of
PIPETMAN M Connected that
you are using (preferably use
PIPETMAN Tips for assurance
of accurate and precise
results; refer to Chapter 9

PIPETMAN DIAMOND Tips on

page 15).

5. Press push button to aspirate
total volume. The volume
aspirated will be a little bit more
than required (extra volume).
The extra volume is necessary to
ensure equal operating conditions
for each dispensed aliquot.

Dispense

Press the push button. DISCARD appears on the screen. Discard the extra volume
andcontinue dispensing the aliquots. PIPETMAN M Connected distributes the aliquot
volume each time you click the push button. The number of aliquots left to dispense is
displayed on the screen. A warning beep indicates the last aliquot.

Purge

Press the push button, PURGE appears on the screen, click again to purge and reset
the piston. Keep your thumb pressing the push button until the end of the purge: the
piston reset takes place after you release the push button again. This feature enables
you to remove the tip from the liquid, without aspirating anything.

Eject Tip

Pressing the ejection button.

MIX Mode

This is the classic pipette mode (forward pipetting) followed by a mixing phase,
composed of repeatedly aspirating and dispensing, as well as an optional forward
pipetting step.

1. Press menu button to access menu, where you can choose between different
pipette modes. Select MIX Mode by turning the push button, then to confirm your
selection click on the push button.

2. Set the first volume, named VOLUME 1: click the push button, set the volume by
turning the push button and click to confirm your selection. This is the first volume
you will aspirate and dispense. Set the second volume, named VOLUME 2: click the
push button, you can now set the second volume by turning the push button.
Click again to confirm your settings.

NOTICE

Always fit a tip
before using any
pipette.

START button Optional STOP

V (+ extra vol)*

Purge

Start Purge

V (vol)

0

DISCARD

ALIQUOTS

PURGE

Click to Purge

Discard

Figure 3

REPETITIVE Mode

NOTE

The MIX Mode can be used to prepare a PCR master mix, enzyme restriction mix, protein
solution, oligonucleotide dilution and to mix samples with gel loading buffer and so on…
you can mix two different solutions or homogenize one solution. If you work with higher
viscosity than water (e.g., restriction enzyme), you may change the aspiration speed
(refer to Chapter 8 Pipetting Speed Control on page 15). If you work with genomic DNA ,
then you should always pipette very carefully to avoid shearing and nicking.

PIPETTING MODES

10

PIPETTING MODES | PIPETMAN ® M CONNECTED

3. Set the MIX volume: click the push button, set the volume by turning the push
button. Confirm your settings with a simple click. This is the volume which will be
aspirated and dispensed repeatedly.

“MIX”

as long as you press the push button.

4. Set the additional pipetting volume, named VOLUME 3: You can choose a volume to be
aspirated and dispensed after the mixing step, so you can continue routine pipetting.
Click the push button, and then set the volume by turning the push button.
Confirm your settings by a simple click.

5. Fit a tip suitable for the model of PIPETMAN M Connected that you are using
(preferably use PIPETMAN DIAMOND Tips for assurance of accurate and precise
results; refer to Chapter 9 PIPETMAN DIAMOND Tips on page 15).

Aspirate VOLUME 1

Press the push button.

Dispense

Press and release the push button:
three things happen: sample
dispense, automatic purge and
piston reset to zero. Or, keep your
thumb pressing the push button
until the end of the dispense cycle:
the piston reset takes place after you
release the push button again.
This feature enables you to remove
the tip from the liquid, without
aspirating anything.

Aspirate and Dispense the
Second Volume

“VOLUME 2”, repeat steps Aspirate Volume 1 and To dispense.

Mix

Press push button; as long as you keep your thumb pressing the button, the pipette
continues mixing. Release the push button to complete the current mixing cycle.

Purge

Press push button, PURGE appears on the screen, click again to purge and reset the
piston.

Pipette

If you have chosen a VOLUME 3 > 0, you can now aspirate and dispense this volume
by repeating steps 2 and 3.

If you set the VOLUME 3 = 0 µL, then the cycle will stop after the MIX step.

NOTICE

Always fit a tip before using any pipette!

NOTE

NOTE

If you set VOLUME 1 or VOLUME 2= 0 µL, then the step will not appear.

If you set VOLUME 1 and 2 = 0 µL, then the cycle will start directly with the MIX step.

NOTE

If you choose VOLUME 1 = 0, then
step 2 won’t occur, if you choose
VOLUME 2 = 0 µL, then step 4 won’t
occur, as well if you set VOLUME
1 and 2 = 0 µL, then step 1–4 will
disappear. If you set VOLUME 3 =
0 µL step 7, then will not occur.

Figure 4

MIX Mode

START button Optional STOP

0

V

Purge

Start Purge

Push button pressed

V1

V2

V3

PIPETTING MODES

11

PIPETMAN® M CONNECTED | USER’S GUIDE

REVERSE Mode

The REVERSE Mode is reverse pipetting with a manual pipette. During
aspiration, additional liquid is added. After delivery, the excess volume
remains in the tip and is discarded.

The REVERSE Mode is ideal for pipetting viscous liquids; liquids with high vapor
pressure or those that tend to foam. Your PIPETMAN M Connected aspirates a
selected volume and an excess. This excess compensates for the liquid that remains
as a film inside the tip during dispensing. For example: protein extraction, cell lysis,
plasmid isolation, cell culture, buffer preparation and, many others.

Electrophoresis Gels Loading Protocol

1. Electrophoresis gels loading protocol: first change the pipetting speed of your
pipette (refer to Chapter 8 Pipetting Speed Control on page 15). Aspiration can be
done by using the standard speed (by default speed 6). Dispensing should be done
very slowly and carefully, to prevent swirling and spilling of the samples, so select
the lowest speed 1.

1. Press menu button to access system menu, where you can choose between
different pipette modes. Select REVERSE Mode by turning the push button.
To confirm your selection click, the push button.

Set the volume

1. Press the push button, set the volume by turning the push button and press again
to confirm your settings.

2. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use
PIPETMAN DIAMOND Tips for assurance of accurate and precise results; refer to

Chapter 9

PIPETMAN DIAMOND Tips

on page 15).

Aspirate

Press push button to aspirate the selected volume.
An amount of liquid equal to the amount of purged
air is added. The volume aspirated will be a little bit
more than the set volume.

Dispense

Press push button to dispense the volume, the
additional amount of liquid remains in the tip.

Purge

Press push button, PURGE appears on the screen,
press again to purge and reset the piston. Keep
your thumb pressing the push button until the end
of the purge: the piston reset takes place after
you release the push button again.
This feature enables you to remove the tip from
the liquid, without aspirating anything.

Eject tip

Press the ejection button.

NOTICE

Always fit a tip before using any pipette.

0

Purge

START button

Optional STOP

Start Purge

V (+ extra vol)*

Click to Purge

Figure 5

REVERSE Mode

NOTICE

If you purge without pulling out the tip air bubbles could get into your gel!

PIPETTING MODES

12

PIPETTING MODES | PIPETMAN ® M CONNECTED

CUSTOM Mode

The CUSTOM Mode enables personalized pipetting protocol creation on a PC or tablet
for transfer to PIPETMAN M Connected through PIPETMAN M Software, an easy-to-use
software. To use this mode on a PC, the software has to be installed on it.

PIPETMAN M Software is downloadable on www.gilson.com/pipetmanmconnected.

You can also directly download the PIPETMAN M Software Android® app on your tablet.

To install PIPETMAN M software on a PC:

1. Download PIPETMAN M software zip file and extract the installation files.
Then choose the program corresponding to your operating system (Windows® 32-bit
or 64-bit).

2. Double-click the installer and follow the instructions on the screen to complete the
installation.

3. When completed, the PIPETMAN M Software icon will appear on desktop of the PC if
“

Create desktop shortcut

” was selected.

4. If PIPETMAN M Software is not launched automatically, then double-click the
PIPETMAN M icon on the desktop.

Once installed, the PIPETMAN M Software can be run by any user on the installed PC or
tablet.

You can then create, edit, import, or export custom up to 10 personalized pipetting
protocols, easily and rapidly.

To create a protocol, choose tasks in PIPETMAN M software:

● Aspirate: aspirates specified volume into the tip.

● Beep: makes pipette produce a beep sound while selected tasks are executed.

● Dispense: dispenses specified volume from the tip.

● Dispense all: dispenses all remaining volume from the tip followed by a purge action.

● Loop: enables repetition of any task once or more. Tasks between Loop (start) and

Loop (end) will be executed in sequence for the specified number of iterations.

● Mix: aspirates then dispenses a specified volume one or more times.

● Purge: dispenses all remaining volume from the tip followed by purging extra volume.

● Repetitive: dispense liquid into a number of aliquot, where during aspiration, additional

liquid is added and after delivery, the excess volume remains in the tip and is discarded.

● Reverse: handle liquid using reverse pipetting.

● Wait: introduces a wait for a specified number of seconds.

● Wait for click: pauses the protocol until Push button is clicked.

In this mode, the protocols can be automatically executed, avoiding repetitive pushes on
the button, helping to decrease risk of RSI.

To transfer a protocol to your PIPETMAN M Connected pipette, click

Transfer custom

protocols to pipette

and follow the instructions on the screen.

For more information on PIPETMAN M Software, refer to the

User's Guide PIPETMAN M

Software

P/N LT801562/B.

BENEFIT FROM THE BLUETOOTH CONNECTION

13

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 7

BENEFIT FROM THE BLUETOOTH

CONNECTION

PIPETMAN M Connected is a smart Bluetooth connected pipette that stores your
data in the cloud for you to recall. It is compatible with the Gilson Connect platform
of apps and has the ability to be connected to a Bluetooth 4.0 smart-ready tab
or phone or any PC equiped with a Bluetooth key. You can work with your smart
pipette on any of the Gilson apps, according to your daily goals.

PIPETMAN M Connected can be connected to the PIPETMAN M Software and the
Gilson apps either with the USB cable or using the Bluetooth® connection.

Connect via Bluetooth

1. Connect the Bluetooth key (part number F807027) to a USB port. (PC only, not
required when using a tablet).

2. Press the push button on the pipette to switch it on and begin initialization.

3. When initialization is complete, press the menu button on the pipette.

4. Press and hold the push button for 2–3 seconds until the Bluetooth indicator lights
flash and then release the push button.

Associated with TRACKMAN® Connected and its PipettePilot application,
PIPETMAN M Connected will reduce risks of human errors by transferring verifiable
pipetting parameters and commands in the pipette.

Please visit www.gilsonconnect.com to learn more about Gilson applications.

PIPETMAN M Connected has is
own software:
PIPETMAN M Software, and
can be used with TRACKMAN
Connected to reduce risks of
human errors by transferring
verifiable pipetting parameters
and commands in the pipette.

BENEFIT FROM THE BLUETOOTH CONNECTION

14

BENEFIT FROM THE BLUETOOTH CONNECTION | PIPETMAN® M CONNECTED

Connection with the PIPETMAN M Software

When it is necessary to have the pipette connected, the software will prompt you.
If the pipette has been registered previously, it is only necessary to activate the
Bluetooth on the pipette to make the connection; however, if this is the first time the
pipette is being used in the software, it is necessary to register the pipette.

1. From the main menu, select Other administration and then Pipette management.

2. Select next to Registered pipettes.

3. Connect the pipette.

If connecting via Bluetooth:

• On the tablet, select The software searches for pipettes with Bluetooth
activated (indicator lights flashing).

• On the PC, select Connect via BLE. The software searches for pipettes with
Bluetooth activated (indicator lights flashing).

Many devices may be detected, but only one can be connected.

If connecting via USB:

• Use a USB cable to connect the pipette to the PC.

• Select Connect via USB. The connected pipette appears in the Devices list

4. Select the pipette in the Devices list.

5. Select Next.

6. When prompted, enter a name for the pipette. If the pipette has already been
registered, then an informational message will appear.

Current connectivity status is always shown at the bottom left part of the screen where
it is clear if the pipette is:

● USB connected

● Bluetooth connected

If you want to connect to another pipette within the same channel as the first
connected pipette, it will get disconnected and the new one will get connected while
the sign below left remains the same.

For more information about all features PIPETMAN M Software offers you, please read
the help section of PIPETMAN M Software.

● There is no connected
pipette

NOTE

PIPETTING SPEED CONTROL

15

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 8

PIPETTING SPEED CONTROL

PIPETMAN M Connected is set by default on speed 6 (maximum speed). You may need
to change the speed of aspiration or dispensing depending on your application. You
can change aspiration speed and dispensing speed independently of one other: from
very slow to very fast (speed 1 — speed 6). Your speed selection is memorized for each
pipetting mode until you change it again.

1. Press menu button to access system menu, where you can find the different
pipette modes, the speed menu and configuration menu.

2. Select SPEED by turning the push button, to confirm your selection click on
the push button.

3. Set ASPIRATION SPEED: aspiration speed is blinking, set the speed by turning the
push button and click to confirm your selection.

4. Set DISPENSING SPEED: dispensing speed is blinking, set the speed by turning
the push button and click to confirm your selection.

After selecting the speed you go automatically back to pipetting mode.

Chapter 9

PIPETMAN® DIAMOND TIPS

PIPETMAN DIAMOND Tips are made to the highest specifications;
strict quality control is maintained throughout the manufacturing
process. These tips are used to calibrate PIPETMAN M Connected,
therefore for optimum performance we recommend using
PIPETMAN Tips with your PIPETMAN M Connected.

However, PIPETMAN M Connected also offers you a high
compatibility with a large number of other tips. For more
information, please contact your Gilson distributor.

Every PIPETMAN DIAMOND Tip is individually marked with an
identification number (ID). To ensure accuracy and precision,
Gilson’s quality assurance system focuses on the following critical
parameters:

● PIPETMAN Tips are made from pure polypropylene (virgin, metal-free, to avoid
the possibility of contamination). They are available sterilized and with filters.

● Sterilized PIPETMAN Tips are certified free of detectable RNases, DNases, DNA,
RNA, and proteases.

● Optimized shape (revised collar for optimum sealing, thin walls, and fine point),
making them easier to mount, more flexible, with no vortexing, and improved
precision.

● PIPETMAN DIAMOND Tips are free from even microscopic defects, especially
at the orifice. All surfaces are smooth and hydrophobic, thereby avoiding the
excessive retention of liquids that causes poor accuracy and a lack of precision.

ID Number

Figure 6

ID number

BENEFIT FROM THE BLUETOOTH CONNECTION

16

BENEFIT FROM THE BLUETOOTH CONNECTION | PIPETMAN® M CONNECTED

● Mold and cavity references are marked on the collar, ensuring the traceability.
For quality assurance purposes batch numbers appear on all packages (bags and
boxes).

● They form an air-tight seal with the tip holder, preventing the leaks that cause
poor accuracy and a lack of precision.

● PIPETMAN DIAMOND Tips (except filter tips) may be autoclaved at 121°C for 20
minutes at 0.1 MPa.

NOTICE

Do not autoclave PIPETMAN Filter Tips as the filter will be damaged.

NOTE

To ensure the best performance from your Gilson pipette, you shoud
ALWAYS use PIPETMAN DIAMOND Tips (in accordance with ISO8655)
system, because PIPETMAN Tips were used to establish the specifications.

Figure 7

Plastic adapter

Figure 8

P8x10M and P12x10M

Long tips

Short tips

Ejector spacer

Ejector spacer D10

* A plastic adapter is required to eject D10 and
DF10 tips (short tips). The adapter is supplied
with P10M . No adapter is required when using
DL10 and DFL10 tips (long tips).

PIPETMAN M CONNECTED MULTICHANNEL

P8x10M

D10
DL10

DF10
DFL10

0.5–10 µL

P12x10M

P8x20M

DL10
D200

DFL10
DF30

1–20 µL

P12x20M

P8x100M

D200 DF100

10–100 µL

P12x100M

P8x200M

D200
D300

DF100
DF200
DF300

20–200 µL

P12x200M

P8x300M

D200
D300

DF200
DF300

10–300 µL

P12x300M

P8x1200M

D1200 DF1200

50–1200 µL

P12x1200M

PIPETMAN M CONNECTED SINGLE CHANNEL

Model

PIPETMAN
DIAMOND Tips

Volume Range

P10M

D10*
DL10*

DF10
DFL10

0.5–10 µL

P20M

D200 DF30

2–20 µL

P100M

D200 D100

5–100 µL

P200M

D200
D300

DF200
DF300

20–200 µL

P300M

D200
D300

DF200
DF300

20–300 µL

P1200M

D1000
D1200

DF1000
DF1200

100–1200 µL

P5000M

D5000

500–5000 µL

P10mLM

D10mL

1–10 mL

GUIDELINES FOR GOOD PIPETTING

17

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 10

GUIDELINES FOR GOOD PIPETTING

Aspirate and Dispense

Fit new PIPETMAN DIAMOND Tip for the best results (refer to Chapter 19

Specifications on page 31).

Single Channel Model

For single channel models, push the tip holder into the tip
using a slight twisting motion to ensure a firm and airtight
seal.

MultiChannel Model

For multichannel models, PIPETMAN DIAMOND Tips are
best fitted from the patented ROCKY RACK available
only in our TIPACKs and TOWERPACKs. ROCKY RACK
is the dome-shaped part of the pack that contains the
tips. ROCKY RACK makes it easy to securely fit the tips
to a multichannel pipette, ensuring an airtight seal on all
channels without the need to use undue pressure or to
touch the tips.

All Models

Pre-rinse the tip. Pre-rinsing consists of aspirating the first
volume of liquid and then dispensing it back into the same
vessel (or to waste). Subsequent volumes that you pipette will have levels of accuracy
and precision within specifications. Some liquids (e.g., protein-containing solutions
and organic solvents) can leave a film of
liquid on the inside the wall of the tip;
pre-rinsing the tip minimizes any errors
that may be related to this phenomenon.

Hold the pipette vertically and immerse
the tip in the liquid (refer to page 16).
Press the push button to aspirate the set
volume of liquid. Wait a few seconds (time
depends on model, refer to page 16); then
withdraw the pipette tip from the liquid.
You may wipe any droplets away from
the outside of the tip using a medical
wipe; however if you do so, take care to
avoid touching the orifice of the tip.

Place the end of the tip against the inside
wall of the recipient vessel (at an angle of
10° to 40°). Press the push button. Wait
for at least a few seconds before releasing
the push button to expel any residual liquid
from the tip. While removing the pipette
draw the tip along the inside surface of the
vessel.

MODEL

IMMERSION
DEPTH (MM)

WAIT TIME
(SECONDS)

P10M 1

1

P20M 2-3

1

P100M 2-4

1

P200M 2-4 1

P300M 2-4

1

P1200M 2-4

2-3

P5000M 3-6

4-5

P10mLM 5-7

4-5

P8x10M/P12x10M 1

1

P8x20M/P12x20M 2-3

1

P8x100M/P12x100M 2-4

1

P8x200M/P12x200M 2-3

1

P8x300M/P12x300M 2-3

1

P8x1200M/P12x1200M 2-4

2-3

Table 1

Immersion depth and wait time

PERSONALIZE YOUR PIPETMAN® M CONNECTED

18

PERSONALIZE YOUR PIPETMAN® M CONNECTED | PIPETMAN® M CONNECTED

General Guidelines for Good Pipetting

● Make sure that you fit new tips.

● Each new tip should be pre-rinsed with the liquid to be pipetted.

● When aspirating, keep the tip at a constant depth below the surface of the liquid
(refer to table 1 on page 17).

● Change the tip before aspirating a different liquid, sample, or reagent.

● Change the tip if a droplet remains at the end of the tip from the previous
pipetting operation.

● Liquid should never enter the tip holder. To prevent this:

○ Never turn the pipette upside down,
○ Never lay the pipette on its side when there is liquid in the tip(s),

The Gilson Stand Adapter (refer to Parts Checklist and Accessories on page 5) is
recommended for use with the CARROUSELTMand SingleTM Pipette Holder to store
your PIPETMAN M Connected pipette in the vertical position. Alternatively the
POWER CARROUSEL can be used to store and charge up to five PIPETMAN M
pipettes.

● When pipetting liquids with temperatures different to the ambient temperature,
pre-rinse the tip several times before use in order to reach equilibrium between
the temperatures of the liquid and the pipette’s dead-volume.

● For volatile liquids you should saturate the dead-volume by aspirating and
dispensing the liquid repeatedly before aspirating the sample.

After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as
described in Chapter 15 Cleaning and Decontamination on page 28.

The pipette can be used between +4°C and +40°C, but the specifications may vary (refer
to Chapter 19 Specifications on page 31).

● Do not pipette liquids having temperatures above 50°C or below 4°C.

Chapter 11

PERSONALIZE YOUR PIPETMAN® M

CONNECTED

Tip Ejector Extension for Use with P10M

Tip ejector extensions are required to eject D10 tip and are
supplied with P10M pipettes.

The tip ejector extension which is made of PVDF
(polyvinylidene Fluoride), is autoclavable.

To fit a tip ejector extension:

1. Hold the pipette with the push button upright.

2. Hold the extension with the slot upright.

3. Slide the extension over the tip holder.

4. Push the extension firmly onto the end of the tip
ejector until it clicks into place (see opposite).

To fit

To remove

Slot

NOTE

Extreme temperatures can aect accuracy and precision!

Figure 9

Fit a tip ejector

GOOD LABORATORY PRACTICE FEATURES

19

PIPETMAN® M CONNECTED | USER’S GUIDE

To remove a tip ejector extension (see opposite):

1. Hold the pipette in one hand and grip the extension with the other.

2. Gently twist the extension (either direction) and pull it away
from the pipette.

Personal Label – Name and/or
Application Tag

Your PIPETMAN M Connected offers you two options to mark your pipette.
You can personalize your pipette with a name tag and/or an application tag:

Window-tag:

1. Remove the window by inserting a small
screwdriver in the access slot.

2. Position the name tag on the pipette.

3. Clip the window back in place.

Clip tag:

1. Pull off the clip.

2. Position the name tag into the clip.

3. Clamp the clip back in place.

Short
tips

Long
tips

Chapter 12

GOOD LABORATORY PRACTICE FEATURES

Your PIPETMAN M Connected is fully compliant to ISO8655 standard and is CE marked
(for EMC and LDV directives).

PIPETMAN M Connected includes the following good laboratory practice (GLP)
features.

General

● Lockable volume.

● Volume-range is printed on the push button.

● Volume range is displayed on the screen.

● Serial number is engraved on the body (handle) of the pipette and encoded in the
firmware.

● Bar code: on the box and with the certificate (can be transferred).

Personalization by nametags (for marking application and/or user name), refer to
Chapter 11 Personalize your PIPETMAN M Connected on page 18.

Cycle Counters

● From last volume setting (to count the number of cycles in the current “run”).

● From manufacture and since the last service (refer to
Chapter 15 Maintenance on page 22).

● By indicators (R1, R2, R3 … Rx) each time pipette is readjusted.

Maintenance intervals can be specified by weeks or number of cycles elapsed.

Figure 10

Remove a tip ejector

Window-tag

Clip tag

Figure 11

Name and application tag

POWER MANAGEMENT

20

POWER MANAGEMENT | PIPETMAN ® M CONNECTED

Alarms

● Low battery warning.

● Service is overdue warning (refer to Chapter 15 Maintenance on page 22).

● Service alert can be set

“On”

or

“Off ”.

Chapter 13

POWER MANAGEMENT

PIPETMAN M Connected battery has been
designed to ensure 900 pipetting cycles
(750 for PIPETMAN M Connected Multi
200 µL and 300 µL) at maximum speed (up
to nine 96–well plates) between two charge
cycles. For service continuity when needing
more capacity, your PIPETMAN M Connected
can also be used while charging with the same
performance as a fully charged pipette.

Battery Charging

PIPETMAN M Connected charges 80% of its full
battery capacity in less than an hour, and it takes
three hours to fully charge the battery.

Charge your pipette by using the power supply:

1. Connect the AC adapter to a suitable
AC power supply.

2. Plug the adapter into the USB port
of the pipette.

The pipette starts charging.
Charge your pipette by using stand adapter (refer to

Chapter 2 Parts Checklist and Accessories on page

5):

1. First place the stand adapter on your Gilson Single
Pipette Holder or Carrousel.

2. Remove the plug. Now place your pipette on the
Stand Adapter and plug the power transformer into
the stand adapter.

The pipette starts charging.

Battery charging of PIPETMAN M Connected using
the POWER CARROUSEL:

1. First install the POWER CARROUSEL as described
above.

2. Remove the plug. Now place your pipette on the
charging position, please ensure
that the pipette is properly fitted to the contacts on
the top of the carrousel
(a proper fitting will trigger a beep sound).

The pipette starts charging.

Stand adapter

Plug

USB port

Figure 12

PIPETMAN® M Connected Power supply

Figure 13

POWER CARROUSEL for
PIPETMAN® M Connected

POWER MANAGEMENT

21

PIPETMAN® M CONNECTED | USER’S GUIDE

Working with Charger Connected to the Pipette

PIPETMAN M Connected can also be used for pipetting while charging.

1. Plug the power supply into your pipette.

2. Press the push button to start your pipette.

PIPETMAN M Connected is ready to pipette.

Low Battery Warning

PIPETMAN M Connected has a low battery alert. As the battery runs
down, the low battery message appears. The warning is replaced by a
blinking symbol of an empty battery. If it is not recharged, the pipette will switch off
after a while. Settings will not be lost.

If the battery is completely discharged (= black screen, no operation) connect the
pipette to the charger for at least five minutes, and then a “batt. too low” message
will appear. The pipette will display the last settings used a few minutes later and will
continue charging.

Changing the Battery

If the following symptoms occur, please contact your Gilson Service Center:

● PIPETMAN M Connected battery won’t charge or will not operate even if
connected to the charger at least for 10 minutes.

● PIPETMAN M Connected battery provides you very short cycle time or
permanently needs to be used in connected mode.

NOTE

You can also recharge PIPETMAN M Connected by USB with your PC; however it
will take much longer than by power supply.

NOTICE

Use only the original power transformer supplied by Gilson. Use of an
incompatible power supply can damage your PIPETMAN M Connected!

Do not operate PIPETMAN while connected to a PC.

CAUTION

PIPETMAN M Connected uses a Lithium-Ion battery. Operation on the battery is
done at user’s risk only. Opening the pipette voids the warranty.

CONFIGURATION

22

CONFIGURATION | PIPETMAN® M CONNECTED

Chapter 14

CONFIGURATION

Enter the menu using the menu button. You will find not
only the pipetting programs and the speed control, but also
the menu

“CONFIGURATION

”, including “

SERVICE”

and

“

ADJUSTMENT”

(refer to Chapter 15 Maintenance below).

The navigation of the menus is always done in the same way
—make your selection by rotating the push button and then
enter and confirm with a click.

The Configuration menu allows you to set up the following items:

● Volume limit: Here you can fix the maximal volume of the
pipette (by default = nominal volume). For example, you
have a P200M pipette, but you would like to use it with
your PIPETMAN Diamond Filter Tips DF100, you can set
the maximal volume at 100 µL and you won’t risk contamination of your pipette.

● Beeper: You can switch the tone of your pipette on or off.

● Contrast: You can choose a value of 1 to 5 to set up the contrast

of the screen.

● Service: refer to Chapter 15 Maintenance below

Adjustment: refer to Chapter 15 Maintenance below.

● Language: Available from the firmware version 1.20.

Languages available are English, Chinese and Japanese.
To change the language, enter in the Configuration menu:

1. Select your language by turning the push button,

2. To confirm your selection, click on the push button,

3. The language selected is blinking,

● To confirm your selection, click 2 seconds on the menu button.

Menu button

Push button

Chapter 15

MAINTENANCE

PIPETMAN M Connected requires very little maintenance. However, to ensure pipette
accuracy, precision and robustness please proceed periodically with a two-minute
inspection as recommended by Gilson.

Your PIPETMAN M Connected allows you to:

● Get service information.

● Readjust the pipette to user settings.

● Return to factory settings.

CONFIGURATION

23

PIPETMAN® M CONNECTED | USER’S GUIDE

Service Information

PIPETMAN M Connected provides you with all required service information. It will help
you establish an easy diagnosis and plan any service operation with your accredited
Gilson service center.

You will find in the Service menu following sub menus:

● Disassembly

● Service info

● Service settings

● Pipette info

The navigation of the menu is always done in the same way. Make your selection by
rotating the push button, enter and confirm with a simple click.

● Disassembly:

○ The multichannel models should not be disassembled: only the push button

and the tip ejector can be replaced.

○ Only the lower part of the single models can be disassembled, the push button,

the connecting nut, and the tip ejector can be replaced.

○ This option allows you to disassemble the lower part safely (protection of the

piston and the actuator). If you select

Disassembly,

the following screen appears:

“Now, you can remove the lower part of your pipette with complete security”.

● Service info provides you with maintenance information; you cannot modify any of
the items:

○ Number of pipetting cycles since last maintenance.
○ Number of weeks since last maintenance.

● Service settings gives you the option to organize your maintenance, you can set
following service points:

○ Number of cycles until next maintenance.
○ Number of weeks until next service.
○ Service date.
○ Activate or deactivate the Service alert.

● Pipette info provides information about your pipette, you cannot modify any of
the items:

○ Version of firmware.
○ Serial number.
○ Total number of cycles (one cycle: up and down of the piston).
○ Number of readjustments carried out by this pipette.

NOTE

Changes of service settings will reset all the service info to zero.

● Replace spare parts (for detailed information refer to Chapter 15 Maintenance on
page 22 and Safety Precautions and Limitation of Use on page 33).

● Prepare the pipette for cleaning or autoclaving by “disassembly” of the parts
specified (refer to Chapter 16 Cleaning and Decontamination on page 28).

CONFIGURATION

24

CONFIGURATION | PIPETMAN® M CONNECTED

Adjustment

Press the menu button to enter the menu. Select

“Configuration”

by rotating the push
button and click to confirm your selection. Enter the sub-menu in the same way and
select the

“Ad j ustment

” menu.

The adjustment menu allows you to access the following items:

● Standard readjustment

● Reset settings

Standard Readjustment

This menu allows you to adjust the pipette using three calibration points: 10%, 50%,
and 100% of nominal volume (in according with ISO8655 recommendations).

You may want to calibrate your pipette for solutions with a density, viscosity, surface
tension or vapour pressure that are different than that of water. To return to the
factory settings, choose

Reset settings.

Your PIPETMAN M Connected will be reset
to the three factory calibration values that are permanently stored in the pipette’s
firmware.

In accordance with ISO 8655 Gilson recommends a gravimetric procedure for
pipette calibration. This gravimetric method is used to establish the mean mass
of a given volume of water (taking into account evaporation losses, where
necessary). After converting the mean mass to a volume (using the Z factor, refer to

Appendix B - Z Factor on page 36), enter the measured volumes(s) into the pipette’s

memory and the software readjusts the pipette accordingly. This method requires
the strict monitoring of environmental conditions and the use of routinely controlled
equipment that is adapted to the volume being measured.

Conversion to volume must take into account the density of the liquid as well as
evaporation during the cycle time. For each measurement, the corresponding volume
(Vi) can be calculated as follows:

Wi is the weight as read on the balance

e is the mean evaporation loss during the cycle

time

Z expressed in L/mg, is a conversion factor incorporating density of water buoyed in
air, at test temperature and barometric pressure (refer to Appendix B - Z Factor on
page 36)

For volumes greater than 20 μL, the evaporation factor can be disregarded.

For more information please refer to

“Verification procedure for accuracy and

precision”

which you can download from the Gilson website (www.gilson.com).

Maintenance Operations

Disassembly of your PIPETMAN M to change and clean parts

It is best to inspect your pipette regularly and to routinely clean and change parts
as required. To help you to keep up a regular schedule and in the interests of good
laboratory practices (GLP), you can configure your pipette to display an alarm before
servicing is due (refer to page 25).

Vi = (Wi + e) Z

NOTE

The following maintenance operation should only be done when the pipette is in
Service menu (refer to page 23).

CONFIGURATION

25

PIPETMAN® M CONNECTED | USER’S GUIDE

Maintenance Warning

PIPETMAN M Connected notifies you when
maintenance is due (Service alert is set by
default to

“On”

). The following message 1

will appear:

After validation by a click, the following message 2 appears:

1. If you click to confirm within 10 seconds. The pipette will automatically switch to
Disassembly mode so you can carry out your maintenance safely.

2. Wait longer than 10 seconds and the option to have a reminder in one week will
appear 3.

1

2

3

3. Click to confirm. Your pipette will be operational again.

Maintenance Operation for Single Channel Models

Tip Holder and Tip Ejector

These parts must be changed if they are damaged. You may also
remove these parts for cleaning or decontamination purposes.

Changing the Tip Ejector

1. Keep the tip ejector button depressed and grip the top of the tip
ejector with the other hand.

2. Gently rotate the tip ejector counterclockwise and separate its
connector from the operating rod.

3. Pull the tip ejector away from the body of the pipette.

4. Clean or autoclave the tip ejector and refit or replace it by reversing the procedure.

Changing the Tip Holder (Lower Part)

After removing the tip ejector, you may remove the lower
part of the tip holder, which is more likely to become
contaminated or damaged than the upper part. Removal
of the lower part is shown below; for the upper part refer
to Removing the Piston on page 24 (special precautions
are necessary).

1. Gently rotate the lower part of the tip holder
counterclockwise to unscrew it from the upper part.

2. Separate the parts and remove the O-ring (refer to

Changing the Seal and/or O-ring on page 26)

3. Clean, and if required, autoclave the lower part of the
tip holder (autoclaving: 20 min. at 121°C and 0.1MPa)

1. If required, lubricate the piston (refer to

How to Lubricate the Piston on page 27) and fit a

new O-ring.

2. Screw the two parts together, making sure that the two parts are fully tightened, by hand.

3. Refit the tip ejector.

Piston

Tip holder
(lower)

Tip holder
(upper)

Figure 14

Ejector clip

Figure 15

Changing the tip holder

NOTICE

After autoclaving the tip holder and tip ejector may change color, however this
has no impact on the performance.

CONFIGURATION

26

CONFIGURATION | PIPETMAN® M CONNECTED

Changing the Seal and/or O-ring

The O-ring is positioned on the piston; it should not be
autoclaved, if worn or damaged in any way, it must be replaced
(refer to Replacement Parts on page 34).

To access the O-ring, remove the tip ejector and unscrew the lower
part of the tip holder. You should now be able to remove the O-ring
from the piston. Sometimes, the O-ring may be found in the recess
at the top end of the lower part of the tip holder. If required, lubricate the piston (refer
to How to Lubricate the Piston on page 27) then fit a new O-ring by sliding it onto the
piston. Reassemble the pipette. Depending on the pipette model the dimensions of the
O-ring will vary (refer to Chapter 21 Replacement Parts on page 34).

Servicing the Piston

You may remove the piston assembly to clean, lubricate, or change the piston.

Removing the Piston

1. Remove the tip ejector and (optionally)
the lower part of the tip holder. If you
remove the lower part, take care to
remove the O-ring as described in

Changing the seal and/or O-ring above.

2. Unscrew the connecting nut (turn by hand,
counterclockwise).

3. Gently remove the connecting nut and upper
tip holder.

4. Pull off the piston assembly from the body of the pipette — separate the parts
(see below).

5. Clean and autoclave (if required) the piston and holder, together with any
other parts that may need to be treated in the same way (refer to Chapter 16

Cleaning and Decontamination on page 28).

6. Lubricate the piston, see Figure 19 on page 27.

7. Reassemble the piston and guide; then carefully insert the assembly into the
body of the pipette. The guide should hold the piston assembly inside the body of
the pipette. You can hear a

“click”

when the piston is back in place.

O-ring

NOTE

Take care not to touch the piston, and that it is dust-free. If needed, clean the
assembly with a dust-free cloth.

Figure 16

O-ring

Figure 17

Piston

NOTE

The specifications of the pipette should be checked after changing any part!

In the case of P1200M, the piston holder, piston, and guide are a unit — do
not try to separate them.

.

NOTICE

Don't pull on the piston to remove the assembly.

CONFIGURATION

27

PIPETMAN® M CONNECTED | USER’S GUIDE

8. Reassemble the upper part of the tip holder and the connecting nut, and then
refit to the body of the pipette by rotating the connecting nut clockwise until it is
finger tight. Fit the O-ring (for P10M, the seal) and reassemble the lower part of
the tip holder. Refit the tip ejector.

The seals for P10M are fragile and can be used only once; therefore, after unscrewing
the lower part of the tip holder you must fit a new seal.

How to Lubricate the Piston (except P10M)

Use only Gilson lubricant (P/N: F2070902, as supplied).

Squeeze a small quantity from the tube onto a clean, lintless cloth. Use the cloth to
transfer the lubricant to the piston. Ensure that the piston is evenly lubricated and that
you wipe away any excess — remember only a fine film of lubricant is required (over
the entire piston).

Maintenance Operation for Multichannel Models

For multichannel models, the lower part should not be disassembled: only the push
button, the connecting nut, and the tip ejector can be replaced.

Changing the Tip Ejector

To remove the tip ejector, keep both
ejector locks depressed. Pull the tip ejector
down.

To refit the tip ejector, gently
re-insert the tip ejector vertically
into the rails of the ejector
support. Pull lightly on the tip
ejector to check the position.

Replace the Ejector Spacer

1. Gently press the tabs from
the ejector spacer and
remove it from the tip
ejector.

2. Insert the ejector spacer and
click it to the tip ejector.

Tip holder (lower) Tip holder (upper)

Connecting
nut

Piston
holder

Piston

Guide

Figure 18

Tip holder upper part

Figure 19

Tip ejector and tip ejector spacer

CLEANING AND DECONTAMINATION

28

CLEANING AND DECONTAMINATION | PIPETMAN® M CONNECTED

Chapter 16

CLEANING AND DECONTAMINATION

PIPETMAN M Connected is designed so that the parts normally in contact with
contaminants can easily be cleaned and decontaminated.

As part of your quality system, you may have procedures for pipette decontamination.
We recommend that you refer to Decontamination Procedures for Gilson Pipettes,
which you can download from Gilson’s website.

If you use chemical solutions for decontamination or detergents for cleaning, other
than those specified below, you should check with your supplier that the solution or
detergent is safe for use with one of the following materials: PP (Polypropylene), PBT
(Polybutylene Terephthalate), PC (Polycarbonate), POM (Polyoxymethylene), PVDF
(Polyvinylidene Fluoride), PEI (Polyetherimide) and stainless steel.

Cleaning

The pipette must be cleaned, as described below, before it is decontaminated. For
cleaning your PIPETMAN M Connected you may use a simple soap solution or any of
the solutions mentioned in Decontamination Procedures for Gilson Pipettes.

Single Channel Models

External

1. Remove the tip ejector for cleaning (refer to Chapter 15 Maintenance on page 22).

2. Replace the elastomer plug to protect battery charging port.

3. Wipe the entire pipette with a soft-cloth or lint-free tissue impregnated with soap
solution, to remove all dirty marks. If the pipette is very dirty, a brush with soft
plastic bristles may be used.

4. To rinse, wipe the entire pipette with a soft-cloth or lint-free tissue impregnated
with distilled water.

5. Leave to air dry.

Internal

The following components only can be immersed in a cleaning solution: tip ejector,
tip holder, connecting nut and piston.

1. Disassemble the pipette as described in Chapter 15 Maintenance on page 22).

2. Set aside the handle in a dry and secure location.

3. Clean the individual components using an ultrasonic bath (20 minutes at 50°C) or
with a soft cloth and brushes.

NOTICE

Use alcohol (ethanol or isopropanol) to clean up the dirt and dust on the tip
holder.

Liquid must not enter the handle of the pipette.

CLEANING AND DECONTAMINATION

29

PIPETMAN® M CONNECTED | USER’S GUIDE

4. Rinse the individual components with distilled water.

5. Leave the parts to dry by evaporation or wipe them with a clean soft cloth or
lint-free tissue.

6. Lubricate the piston and reassemble the pipette according to the instructions
given in Chapter 15 Maintenance on page 22).

Multichannel Models

The following components only can be immersed in a cleaning solution: tip ejector,
ejector locks, and ejector spacer.

1. Remove the tip ejector and the ejector spacer (refer to Chapter 15 Maintenance on
page 22).

2. Immerse the tip ejector, ejector locks, and ejector spacer in the cleaning solution
or wipe them with a soft cloth or lint-free tissue impregnated with the cleaning
solution.

3. Rinse the components with distilled water.

4. Leave the parts to dry by evaporation or wipe them with a clean, soft cloth or
lint-free tissue.

5. Refit the tip ejector.

Decontamination

Autoclaving – Single Channel Models

However, after separation from the body, any of the following components of the
volumetric module may be autoclaved individually: tip ejector, connecting nut, tip
holders, pistons, and seals (except O-rings).

1. Clean the parts to be autoclaved, especially the tip holders.

2. Put the parts in an autoclaving bag.

3. Autoclave for 20 minutes at 121°C and 0.1 MPa.

4. Check that the parts are dry before reassembling the pipette.

Set the pipette aside to stabilize at room temperature for at least 6 hours.

Chemical Decontamination – Single and
Multichannel Models

You may choose to decontaminate your pipette chemically, in accordance with your
own procedures. Whatever decontaminant you use, check that it is compatible with
the plastics used in the construction of the pipette (refer to page 24).

NOTICE

The body (handle) of the pipette is not autoclavable.

The specifications of the pipette should be checked after autoclaving/
disassembling.

NOTE

Please note that although the lower part of PIPETMAN M Connected multichannel
can withstand a few autoclaving cycles, we do not recommend autoclaving it.

LEAK TEST

30

LEAK TEST | PIPETMAN® M CONNECTED

Non-Immersible Parts

1. Wipe the handle of the pipette with a soft cloth or lint-free tissue covered with
the chosen decontaminant.

2. Wipe the handle of the pipette with a soft cloth or lint-free tissue covered with
distilled water.

Immersible Parts

Only the following components can be immersed in a decontaminant solution:

● Single channel models a tip ejector, tip holder (both parts), connecting nut,
piston (including holder), and guide.

● Multichannel models a tip ejector, ejector locks, and ejector spacer.

Please note that although the lower part of PIPETMAN M Connected multichannel can
withstand a few number of autoclaving cycles, we do not recommend autoclaving it.

1. Disassemble the pipette as described in Chapter 15 Maintenance on page 22).

2. Immerse the components in the decontaminant solution or wipe them according
the intructions given by the manufacturer or supplier of the decontaminant.

3. Rinse the individual components with distilled or sterile water.

4. Leave the parts to dry by evaporation or wipe them with a clean, lint-free tissue
or soft cloth.

5. Lubricate the piston and reassemble the pipette according to the instructions
given in this chapter.

Chapter 17

LEAK TEST

This test may be performed at any time to check that the pipette does not leak,
especially after performing a maintenance or decontamination procedure. If a pipette
fails this test, you should replace the faulty part (e.g., O-ring, tip holder) and repeat this
test, after making sure that the pipette is correctly reassembled.

1. Fit a PIPETMAN DIAMOND Tip.

2. Set the pipette to the nominal volume.

3. Aspirate the nominal volume from a beaker of distilled water.

4. Hold the pipette in the vertical position and wait for 20 seconds.

If a water droplet appears at the end of the tip, there is a leak (refer to Chapter 18

Troubleshooting on page 31)

If you see no droplet, re-immerse the tip below the surface of water.

The water level inside the tip should remain constant; if the level goes down then there is a
leak (refer to Chapter 18 Troubleshooting on page 31)

For multichannel models, check if the water level between each tip is the same.

TROUBLESHOOTING

31

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 18

TROUBLESHOOTING

In case of malfunction, first reset the pipette by pressing on the push button and the
menu button simultaneously for at least eight seconds.

If the problem persists, you may consult the following table that identifies potential
problems and their solutions.

WARNING

Before returning any pipette to your local Gilson service center, ensure that
it is completely free of chemical, biological, or radioactive contamination.
Please use the included safety bag.

Chapter 19

SPECIFICATIONS

PIPETMAN M is a high quality pipette that offers excellent accuracy and precision; it is
fully compliant with ISO 8655 and is CE marked.

The figures given in the Gilson Maximum Permissible Errors Table 2 on page 32 were
obtained using “PIPETMAN DIAMOND Tips”. These figures are only guaranteed when
using genuine PIPETMAN DIAMOND Tips.

Each pipette is inspected and validated by qualified technicians according to the Gilson
quality system. Gilson declares that its manufactured pipettes comply with the
requirements of the ISO 8655 standard, by type testing.

PROBLEM POSSIBLE CAUSE PAGE

Pipette is leaking
sample

Worn O-ring

26

Pipette won’t
aspirate

Unscrewed lower part
of tip holder

26

Damaged or corroded piston

26

Damaged tip holder

25

Improper repair or assembly

24–27

Connecting nut is loose.

26–27

Software needs to be reset

6

Noisy operation Piston needs lubricating

27

Pipette is
inaccurate

Improper repair or assembly

24–27

Unscrewed lower part
of tip holder

26

Pipette is out of adjustment

24

Connecting nut is loose

26–27

PROBLEM POSSIBLE CAUSE PAGE

Pipette is not precise

Unscrewed lower part of tip holder

26

Incorrect operator technique

17

Worn O-ring

26

Connecting nut is loose

26–27

Damaged or corroded piston

26

Damaged tip holder

25

Tips fall or don’t fit

Low quality tips

15–16

Damaged tip holder

25

Damaged tip ejector

25–27

Ejector spacer is damaged

27

Tip ejector is loose

25–27

Dirty tip holder

25

No OLED display

Pipette is in sleep mode

5

Battery needs recharging

15

Software needs to be reset.

6

No operation possible

Battery needs recharging.

15

Software needs to be reset 6

Calibration impossible Software needs to be reset

6

TROUBLESHOOTING

32

TROUBLESHOOTING | PIPETMAN® M CONNECTED

The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6):

● Basis of adjustment, Ex.

● Reference temperature, 20°C

● Relative humidity, 50%

● Barometric pressure, 101 kPa

● Use of distilled water grade 3 (ISO 3696)

● Ten measurements for each test
volume, which are nominal volume,
50% of nominal volume and 10%
of nominal volume. (tested mode:
PIPET, speed 6 with
PIPETMAN DIAMOND Tips)

Table 2

PIPETMAN® M Connected maximum permissible errors

Gilson maximum permissible errors are guaranteed only when PIPETMAN pipettes are used with the recommended
PIPETMAN DIAMOND Tips. Visit www.gilson.com/en/guaranteedperformance.

Under these conditions, Gilson volumetric specifications in standard pipetting (PIPET mode) are guaranteed with a performance
exceeding ISO 8655-2 recommendations for this mode.

*These PIP ETMAN DIAMON D Tips can be used with the indicated pipettes for th e REPETITIVE mode but not until the maximal volume of
the pipette in PIPET mode . Please refer to the volume ran ge of your tips.

Recommended tips to get best results in REPETITIVE mode.

PIPETMAN M CONNECTED SINGLE CHANNEL — VARIABLE VOLUME MODELS

Model

PIPETMAN
DIAMOND Tips

Part
Number

Gilson Specifications

ISO 8655-2

Standard PIPET mode

REPETITIVE
mode
volume

Systematic
error (µL)

Random
error (µL)

Volume Range

Volume
(μL)

Systematic
error (μL)

Random
error (μL)

P10M

D10
DL10

DF10
DFL10

F81022

0.5–10 µL

0.5
1
5
10

± 0.040
± 0.025
± 0.060
± 0.080

≤ 0.013
≤ 0.012
≤ 0.020
≤ 0.025

0.5–10 µL

± 0.12
± 0.12
± 0.12
± 0.12

≤ 0.08
≤ 0.08
≤ 0.08
≤ 0.08

P20M

D200 DF30

F81023

2–20 µL

2
10
20

± 0.075
± 0.100
± 0.150

≤ 0.025
≤ 0.035
≤ 0.050

2–20 µL

± 0.2
± 0.2
± 0.2
± 0.2

≤ 0.1
≤ 0.1
≤ 0.1
≤ 0.1

P100M

D200 D100

F81013

5–100 µL

5
10
50
100

± 0.35
± 0.30
± 0.38
± 0.40

≤ 0.10
≤ 0.10
≤ 0.12
≤ 0.15

5–100 µL

± 0.8
± 0.8
± 0.8
± 0.8

≤ 0.3
≤ 0.3
≤ 0.3
≤ 0.3

P200M

D200

D300

DF200
DF300

F81024

20–200 µL20100

200

± 0.40
± 0.80
± 1.00

≤ 0.15
≤ 0.22
≤ 0.26

5 – 200 µL

± 1.6
± 1.6
± 1.6

≤ 0.6
≤ 0.6
≤ 0.6

P300M

D200

D300

DF200
DF300

F81014

20–300 µL

20
30
150
300

± 0.80
± 0.70
± 0.90
± 1.05

≤ 0.16
≤ 0.20
≤ 0.23
≤ 0.30

10–300 µL

± 4.0
± 4.0
± 4.0
± 4.0

≤ 1.5
≤ 1.5
≤ 1.5
≤ 1.5

P1200M

D1000

D1200

DF1000
DF1200

F81015

100–1200 µL

100
120
600
1200

± 2.5
± 2.4
± 3.6
± 6.0

≤ 0.4
≤ 0.4
≤ 0.8
≤ 1.2

20–1200 µL

± 16
± 16
± 16
± 16

≤ 6.0
≤ 6.0
≤ 6.0
≤ 6.0

P5000M

D5000

F81016

500–5000 µL

500
2500
5000

± 10
± 15
± 25

≤ 2
≤ 4
≤ 7

100–5000 µL

± 40
± 40
± 40

≤ 15.0
≤ 15.0
≤ 15.0

P10mLM

D10mL

F81017

1–10 mL

1 mL
5 mL
10 mL

± 25
± 30
± 50

≤ 4
≤ 8
≤ 12

200 µL–10 mL

± 60
± 60
± 60

≤ 30.0
≤ 30.0
≤ 30.0

PIPETMAN M CONNECTED MULTICHANNEL

P8x10M

D10
DL10

DF10
DFL10

F81025

0.5–10 µL

0.5
1
5
10

± 0.05
± 0.04
± 0.08
± 0.10

≤ 0.02
≤ 0.02
≤ 0.04
≤ 0.06

0.5–10 µL

± 0.24
± 0.24
± 0.24
± 0.24

≤ 0.16
≤ 0.16
≤ 0.16
≤ 0.16

P12x10M F81026

P8x20M

DL10

D200

DFL10
DF30

F81027

1–20 µL

1
2
10
20

± 0.08
± 0.09
± 0.15
± 0.25

≤ 0.05
≤ 0.06
≤ 0.10
≤ 0.12

1–20 µL

± 0.4
± 0.4
± 0.4
± 0.4

≤ 0.2
≤ 0.2
≤ 0.2
≤ 0.2

P12x20M F81028

P8x100M

D200 DF100

F81018

10–100 µL

10
50
100

± 0.25
± 0.50
± 0.80

≤ 0.14
≤ 0.20
≤ 0.25

5–100 µL

± 1.6
± 1.6
± 1.6

≤ 0.6
≤ 0.6
≤ 0.6

P12x100M F81019

P8x200M

D200

D300

DF100
DF200
DF300

F81029

20–200 µL

20
100
200

± 0.50
± 1.00
± 2.00

≤ 0.16
≤ 0.30
≤ 0.50

5–200 µL

± 3.2
± 3.2
± 3.2

≤ 1.2
≤ 1.2
≤ 1.2

P12x200M F81030

P8x300M

D200

D300

DF200
DF300

F81031

10–300 µL

10
30
150
300

± 1.00
± 1.00
± 1.50
± 2.40

≤ 0.18
≤ 0.18
≤ 0.375
≤ 0.45

10–300 µL

± 8.0
± 8.0
± 8.0
± 8.0

≤ 3.0
≤ 3.0
≤ 3.0
≤ 3.0

P12x300M F81032

P8x1200M

D1200 DF1200

F81020

50–1200 µL

50
120
600
1200

± 4.0
± 4.0
± 6.0
± 9.6

≤ 0.7
≤ 0.7
≤ 1.5
≤ 1.8

50–1200 µL

± 32
± 32
± 32
± 32

≤ 12
≤ 12
≤ 12
≤ 12

P12x1200M F81021

SAFETY PRECAUTIONS AND LIMITATIONS OF USE

33

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 20

SAFETY PRECAUTIONS AND

LIMITATIONS OF USE

For safety reasons, it is important to observe the following instructions:

● Battery and electrical specifications:

•

•

Use AC adaptor and stand adapter indoors.

When pipetting infectious, radioactive, toxic and other hazardous solutions, please
observe all the safety precautions (e.g. wear protective clothing, goggles and gloves) and
regulations appropriate for your country.

● Storage conditions
Temperature: -20°C to 50°C - Humidity max: 80%

●

Temperature of use

Between 4°C and 40°C (specifications may vary).

●

Use only genuine PIPETMAN DIAMOND Tips and original Gilson’s accessories

and spare parts. If the equipment is used in a manner not specified by Gilson in
the user’s guide, the protection provided in the equipment may be impaired

.

● Equipment disposal
This equipment must not be disposed of with unsorted municipal waste. Instead, it is
your responsibility to correctly dispose of your waste equipment by handing it over to
an authorized facility for separate collection and recycling. It is also your responsibility
to decontaminate the equipment in case of biological, chemical, and/or radiological
contamination so as to protect from health hazards the persons involved in the
disposal and recycling of equipment. For more information about where you can drop
off your waste equipment for recycling, please contact your local dealer from whom
you originally purchased the product or your local council. By doing so, you will help
conserve natural resources and you will ensure that your waste equipment is recycled
in a manner that protects human health and the environment. Thank you.

● PIPETMAN M Connected can be used indoor and outdoor,if precautions are respected
as described above in this handbook and in the good laboratory practices (GLP).

NOTICE

We strongly recommend you fully charge the battery before using the pipette.
PIPETMAN M Connected is supplied with an AC adaptor that is suitable for
your country. You must only use an original Gilson AC adaptor specific to this
product. Charge the battery in the pipette, using the AC-adapter or the stand
adapter.

WARNING

Do not use PIPETMAN M Connected in a potentially explosive environment or
with potentially explosive chemicals.

NOTICE

Do not allow the liquid to enter the body of the pipette

CAUTION

PIPETMAN M Connected uses a Lithium-Ion battery. Operation on the
battery is done at user’s risk only. Opening the pipette voids the warranty.
Dispose of used batteries in accordance with legal regulations. Batteries
may not be disposed of with household waste and may explode if
disposed in fire.

● Li-ion battery pack; 1 Ah / 3.6 V. Charging time: approx. 3 hours for a
fully discharged battery (80% in one hour).

● AC adaptor : Input voltage, country-specific: 100-240 V, 50/60 Hz
(0.5A max.) Output voltage: +5 V DC, 3.5A (17.5W max.)

● Class II this device is double insulated.

REPLACEMENT PARTS

34

REPLACEMENT PARTS | PIPETMAN® M CONNECTED

DESCRIPTION P10M P20M P100M P200M P300M P1200M P5000M P10mLM

Connecting nut F807012 F807012 F807012 F807012 F807012 F807012 F807012 F807012

Battery tag, 1x4 F807013 F807013 F807013 F807013 F807013 F807013 F807013 F807013

Ejector tag, 1x4 F807014 F807014 F807014 F807014 F807014 F807014 F807014 F807014

Power supply USB F807015 F807015 F807015 F807015 F807015 F807015 F807015 F807015

Power tub cover F807022 F807022 F807022 F807022 F807022 F807022 F807022 F807022

Battery window F807005 F807005 F807005 F807005 F807005 F807005 F807005 F807005

Ejector window F807006 F807006 F807006 F807006 F807006 F807006 F807006 F807006

Lubrican tube 3.5g - F3070902 F3070902 F3070902 F3070902 F3070902 F3070902 F3070902

Seal, 1x5 F161902

- - - - - - -

Seal & O-Ring, 5 sets - F144863

- - - - - -

O-ring, 1x5

- -

F807146 F2070501 F807134 F807152 F807148 F807149

Tip holder, upper part F2070117 F2070117 F807135 F2070517 F2070517 F2070617

- -

Tip holder, lower part F2070218 F2070318 F807136 F2070518 F807153 F2070618 F2070719 F807147

Button assembly F807141 F807142 F807119 F807143 F807120 F807121 F807122 F807123

Tip ejector assembly F807008 F807009 F807130 F807010 F807010 F807011 F807131 F807132

Piston assembly F807017 F807018 F807126 F807019 F807127 F807020 F807128 F807129

Chapter 21

REPLACEMENT PARTS

DESCRIPTION P8X10M P12X10M P8X20M P12X20M P8X100M P12X100M P8X200M P12X200M P8X300M P12X300M P8X1200M 12X1200M

Battery tag, 1x4 F807013 F807013 F807013 F807013 F807013 F807013 F807013 F807013 F807013 F807013 F807013 F807013

Ejector tag, 1x4 F807014 F807014 F807014 F807014 F807014 F807014 F807014 F807014 F807014 F807014 F807014 F807014

Power supply USB F807015 F807015 F807015 F807015 F807015 F807015 F807015 F807015 F807015 F807015 F807015 F807015

Power tub cover F807022 F807022 F807022 F807022 F807022 F807022 F807022 F807022 F807022 F807022 F807022 F807022

Battery window F807005 F807005 F807005 F807005 F807005 F807005 F807005 F807005 F807005 F807005 F807005 F807005

Ejector window F807006 F807006 F807006 F807006 F807006 F807006 F807006 F807006 F807006 F807006 F807006 F807006

Button assembly F807141 F807141 F807144 F807144 F807124 F807124 F807143 F807143 F807145 F807145 F807125 F807125

Ejector spacer F507001 F507003 F507001 F507003 F507001 F507003 F507001 F507003 F507001 F507003 F507139 F507140

Ejector spacer D10 F807117 F807118 - - - - - - - - - -

Ejector lock F507008 F507008 F507008 F507008 F507008 F507008 F507008 F507008 F507008 F507008 F507008 F507008

Tip ejector assembly F507005 F507006 F507005 F507006 F507005 F507006 F507005 F507006 F507005 F507006 F507137 F507138

Ejector
spacer

Ejector lock

Tip ejector
assembly

Multichannel Models

Figure 20

Multichannel ejector

Single Channel Models

APPENDICES

35

PIPETMAN® M CONNECTED | USER’S GUIDE

APPENDICES

Appendix A – Example of a Performance Check

Below is an example of how to evaluate the performance of PIPETMAN M Connected
P10M at 1 µL.

1. Determine the mean value e
of the evaporation loss ei that

occurs during your pipetting
cycles. Proceed as described in

Appendix C - Evaporation Loss on

page 35 to determine ei.

2. Change the pipette tip and perform
the first weighing. Then, keep a

regular cycle and perform the ten
following measurements.

3. Calculate the mean weight

wr = 0.957 mg
w1 = 0.968 mg

w6 = 0.966 mg
w7 = 0.955 mgw2 = 0.960 mg

w8 = 0.972 mgw3 = 0.984 mg
w4 = 0.942 mg
w5 = 0.969 mg

w9 = 0.958 mg

wr rinsing measurement which is

disregarded for the calculation

w10 = 0.967 mg

W =

_

W = (0.968+0.960+0.984+0.942

+ 0.969+0.966+0.955+0.972
+ 0.958+0.967) / 10

_

W =

0.964 mg

_

n number of weighings
W

i

weighing results

Wi

1
n

n

i=1

4. Calculate the mean volume.
For a temperature of 21.5°C and an air
pressure of 1013 hPa, the Z factor is
equal to 1.0032 µL/mg (see table 3 in

Appendix B - Z factor on page 34).

5. Evaluate accuracy

6. Evaluate precision (repeatability)

Standard Deviation (SDW)

V = ( W+e ) x Z

V = (0.964+0.018) x 1.0032

_

V =

0.985 µL

_

E = V - V0

V0 true value set on the instrument
E = 0.985 - 1 = - 0.015 µL

E% = (V - V0) x 100 / V

0

E% = (-0.015 x 100) / 1 = - 1.50 %

Systematic
error (E):

Relative error

(E%):

(

W

i - W

)

n - 1

SDw =

2

n

i=1

n

i=1

SD

w

2

= ( Wi - W )

2

1

n - 1

1

9

SD

w

2

=

SD

w

= 0.011 mg

(0.968-0.964)2+ (0.960-0.964)2+ (0.984-0.964)2+
(0.942-0.964)

2

+ (0.969-0.964)2+ (0.966-0.964)2+

(0.955-0.964)

2

+ (0.972-0.964)2+ (0.958-0.964)2+

(0.967-0.964)

2

SDv = SD

w

x Z

SDv = 0.011 x 1.0032 = 0.011 µL

Random error (SDv):

APPENDICES

36

APPENDICES | PIPETMAN® M CONNECTED

Appendix B – Z Factor

The reference calculation equation is :

Z = [1/(PW-PA)] [1-(PA/PB)]

Where: PA = density of air at t°C.

PW = density of the test liquid at t°C.
PB = density of the balance weights.
Use 8 g/cc for P

B

NOTE

Weights conforming to International recommendation N°33 of OIML have been
adjusted to give results when weighing in air as if the density of the weights were

8.0 g/mL.

Values of the conversion factor Z (µL/mg) as a function of temperature and pressure for
distilled water.

TEMPERATURE AIR PRESSURE (HPA)

(°C) 800 853 907 960 1013 1067

15 1.0018 1.0018 1.0019 1.0019 1.0020 1.0020

15.5 1.0018 1.0019 1.0019 1.0020 1.0020 1.0021

16 1.0019 1.0020 1.0020 1.0021 1.0021 1.0022

16.5 1.0020 1.0020 1.0021 1.0022 1.0022 1.0023

17 1.0021 1.0021 1.0022 1.0022 1.0023 1.0023

17.5 1.0022 1.0022 1.0023 1.0023 1.0024 1.0024

18 1.0022 1.0023 1.0024 1.0024 1.0025 1.0025

18.5 1.0023 1.0024 1.0025 1.0025 1.0026 1.0026

19 1.0024 1.0025 1.0025 1.0026 1.0027 1.0027

19.5 1.0025 1.0026 1.0026 1.0027 1.0028 1.0028

20 1.0026 1.0027 1.0027 1.0028 1.0029 1.0029

20.5 1.0027 1.0028 1.0028 1.0029 1.0030 1.0030

21 1.0028 1.0029 1.0030 1.0030 1.0031 1.0031

21.5 1.0030 1.0030 1.0031 1.0031 1.0032 1.0032

22 1.0031 1.0031 1.0032 1.0032 1.0033 1.0033

22.5 1.0032 1.0032 1.0033 1.0033 1.0034 1.0035

23 1.0033 1.0033 1.0034 1.0035 1.0035 1.0036

23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0037

24 1.0035 1.0036 1.0036 1.0037 1.0038 1.0038

24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039

25 1.0038 1.0038 1.0039 1.0039 1.0040 1.0041

25.5 1.0039 1.0040 1.0040 1.0041 1.0041 1.0042

26 1.0040 1.0041 1.0042 1.0042 1.0043 1.0043

26.5 1.0042 1.0042 1.0043 1.0043 1.0044 1.0045

27 1.0043 1.0044 1.0044 1.0045 1.0045 1.0046

27.5 1.0044 1.0045 1.0046 1.0046 1.0047 1.0047

28 1.0046 1.0046 1.0047 1.0048 1.0048 1.0049

28.5 1.0047 1.0048 1.0048 1.0049 1.0050 1.0050

29 1.0049 1.0049 1.0050 1.0050 1.0051 1.0052

29.5 1.0050 1.0051 1.0051 1.0052 1.0052 1.0053

30 1.0052 1.0052 1.0053 1.0053 1.0054 1.0055

Table 3

Z Factor

APPENDICES

37

PIPETMAN® M CONNECTED | USER’S GUIDE

Appendix C – Evaporation Loss

Procedure for the Determination of Evaporation Loss

Use the same distilled water, weighing vessel and balance as you will be using for the
gravimetric check.

Half fill the weighing vessel with distilled water.

1. Cover the weighing vessel with its lid and place it on the balance using a pair of
tweezers.

2. Aspirate a sample.

3. Tare the balance and take the weighing vessel out of the balance.

4. Take off the lid with tweezers.

5. Dispense the sample into a dummy vessel.

6. Replace the lid on the weighing vessel and, using tweezers, replace the vessel on
the balance.

7. Read the negative result e1 (record the absolute value).

8. Repeat steps 3 to 8, three times to obtain e2, e3, and e4.

9. Calculate the evaporation loss e using the formula:

NOTE

In normal conditions, this value is usually between 0.01 mg and 0.03 mg.

e = (e1 + e2 + e3 + e4)

1
4

REGULATORY COMPLIANCE

38

REGULATORY COMPLIANCE | PIPETMAN® M CONNECTED

Chapter 22

REGULATORY COMPLIANCE

Gilson certifies on its sole responsibility that PIPETMAN M Connected complies
with the requirements of the following European Directives:

2014/30/EU Electromagnetic compatibility, EMC
2014/35/EU Low Voltage Directive, LVD
2014/53/EU Radio Equipment Directive

This Bluetooth enabled device also complies with the following requirements:

USA, User information: Contains FCC ID: 2AAQS-ISP1507
Canada, User information: Contains IC: 11306A-ISP1507
Japan, TELEC certification n°207-16ISP5

The WEEE symbol (crossed-out wheeled bin), according to the
European Directive 2012/19/EU, indicates separate collection for
WEEE – Waste of Electrical and Electronic Equipment.

Do not dispose electronic devices and their batteries in a household
bin, use the recycling path in place in your country.

WARRANTY

39

PIPETMAN® M CONNECTED | USER’S GUIDE

Chapter 23

WARRANTY

Gilson warrants this pipette against defects in material under normal use and
service for a period of 12 months from the date of purchase.

This warranty shall not apply to pipettes which are subject to abnormal use
and/or improper or inadequate maintenance (contrary to the recommendations
given in the user’s guide), including, but not limited to pipettes which have been
subjected to physical damage, improper handling, or spillage or exposure to any
corrosive environment. This warranty shall also be void in the event pipettes are
altered or modified by any party other than Gilson or its designates. Gilson’s sole
liability under this warranty shall be limited to, at Gilson’s sole option, repair or
replacement of any defective components of pipettes or refund of the purchase
price paid for such pipettes.

THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS
ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY
WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS
FOR A PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON
BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL
DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED
WARRANTY.

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LT801594/A | © 2018 Gilson, Inc. All rights reserved.