The electronic pipette with
guaranteed performance
in both standard and
repetitive pipetting.
USER'S GUIDE
EN
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USER’S GUIDE
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PIPETMAN M
USER’S GUIDE
EN
PIPETMAN M
1 INTRODUCTION
Congratulations on the purchase of your PIPETMAN® M, the electronic pipette with guaranteed
performance in both standard and repetitive pipetting modes.
PIPETMAN M is a motorized member of the PIPETMAN® family of pipettes from Gilson, requiring
virtually no pipetting forces to aspirate and dispense samples. Combining PIPETMAN’s renowned
accuracy, precision and robustness with user-friendly functions, PIPETMAN M will reduce pipetting
fatigue and increase pipetting efficiency.
PIPETMAN M is a unique pipette with only two buttons to operate the pipette and reach all menu options:
• Push-button: set the volume by turning the button left or right and pipette with a simple push.
• Mode-button: access additional features like repetitive mode, mix or maintenance functions.
Only two buttons are needed to perform your routine pipetting tasks.
PIPETMAN M features the classic PIPETMAN look with additional functionality:
• Intuitive interface with five pipetting modes for a large number of applications:
standard pipet, repetitive, mix, reverse and custom mode.
•
Minimal effort: aspirate and dispense with one click of the push-button and
eject tips with ease to help reduce the risk of repetitive strain injuries (RSI).
• Maximum comfort: the lightweight and balanced design of PIPETMAN
M offers an ergonomic design that rests comfortably in your hand as
you pipette.
• Minimized pipetting variability: the motorized piston allows for high
reproducibility and accuracy while pipetting.
• Increased performance for all day pipetting comfort.
PIPETMAN M is available in 20 models covering a range from 0.5 µL
to 10 mL in single channel and 0.5 µL to 1200 µL in multichannel:
Page
1 INTRODUCTION 3
2 PARTS CHECK LIST AND ACCESSORIES 4
3 GETTING STARTED 4
4 DESCRIPTION 5
5 PIPETTING JUST LIKE WITH PIPETMAN 6
6 PIPETTING MODES 6
7 PIPETTING SPEED CONTROL 12
8 PIPETMAN TIPS 12
9 GUIDELINES FOR GOOD PIPETTING 14
10 PERSONALIZE YOUR PIPETMAN M 15
11 GOOD LABORATORY PRACTICE FEATURES 16
12 PIPETMAN M POWER MANAGEMENT 17
13 CONFIGURATION 18
14 MAINTENANCE OF YOUR PIPETMAN M 19
15 CLEANING AND DECONTAMINATION 24
16 LEAK TEST 25
17 TROUBLESHOOTING 27
18 SPECIFICATIONS 27
19 SAFETY PRECAUTIONS AND LIMITATIONS OF USE 29
20 REPLACEMENT PARTS 30
APPENDICES 31
EC DECLARATION OF CONFORMITY 52
WARRANTY 54
Mode-button
Push-button
Model Reference
Range (PIPET mode)
Color code
P10M F81022 0.5 µL to 10 µL Red
P20M F81023 2 µL to 20 µL Pale yellow
P100M F81013 5 µL to 100 µL Salmon
P200M F81024 20 µL to 200 µL Yellow
P300M F81014 20 µL to 300 µL Green
P1200M F81015 100 µL to 1200 µL Blue
P5000M F81016 500 µL to 5000 µL Purple
P10mLM F81017 1 µL to 10 mL Pale blue
Model Reference
Range (PIPET mode)
Color code
P8x10M F81025 0.5 µL to 10 µL Red
P12x10M F81026 0.5 µL to 10 µL Red
P8x20M F81027 1 µL to 20 µL Pale yellow
P12x20M F81028 1 µL to 20 µL Pale yellow
P8x100M F81018 10 µL to 100 µL Salmon
P12x100M F81019 10 µL to 100 µL Salmon
P8x200M F81029 20 µL to 200 µL Yellow
P12x200M F81030 20 µL to 200 µL Yellow
P8x300M F81031 10 µL to 300 µL Green
P12x300M F81032 10 µL to 300 µL Green
P8x1200M F81020 50 µL to 1200 µL Blue
P12x1200M F81021 50 µL to 1200 µL B lue
Contents
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USER’S GUIDE
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PIPETMAN M
2 PARTS CHECK LIST AND ACCESSORIES
Contents:
Take a moment to verify that the following items
are in the box:
• PIPETMAN M pipette
• Power Supply 5V with AC adapter and cable
• 4 Battery side tags
• 4 Ejector side tags
• Tip-ejector extension (P10M model only)
• Quick Guide
• Safety bag
• Certificate of conformity (including bar-code
sticker)
• 2-year warranty
• Lubricant (except for P10M, and the
multichannel models)
3 GETTING STARTED
Switch on your PIPETMAN M:
Press the push-button to activate the pipette. The start-up screen appears:
The pipette will do a self calibration test, the firmware resets the piston.
During initialization, displayed on the screen, you will see the volume range,
version of firmware and the number of readjustments.
Your PIPETMAN M is preset on PIPET mode and is now ready to pipette (see
Chapter 5).
PIPETMAN M goes into sleep mode after 3 minutes of inactivity. The display will
shut off. Just press the push-button to reactivate the pipette, your last setting
and pipetting mode will appear on the screen.
4 DESCRIPTION
PIPETMAN M Single and Multichannel
models
Upper part
Push-button and volume adjustment knob
Mode-button: direct access to all menu
functions
Tip-ejector button
Display
Elastomer plug to cover the battery charging
port
Identity-tag clip
Identity-tag window
Connecting nut – attaches handle to lower part
Lower part
Tip-ejector: removable to access tip-holder
Optimized tip-holder to reduce tip tting
and ejection forces, removable for cleaning
and servicing
Ejector clip
Ejector support
Cover
Ejector spacer
Screen
Pipetting Mode
Aspirate and dispense indicator
Battery status
Purge indicator
Volume
Reset
To reset your pipette, simultaneously press on
the push- and mode- button for at least
5 seconds.
Switch off
To switch off your PIPETMAN M, press the pushbutton for at least 5 sec.
Accessories:
The following items are sold separately:
• POWER CARROUSEL: 5-position charging
carrousel for single and multichannel models
FB1001
• Battery side tags (set of 4)
F807013
• Ejector side tags (set of 4)
F807014
Please contact your Gilson Distributor for
these accessories.
Volume
range
Version of
firmware
Number of readjustments
Display
The PIPETMAN M display is an OLED screen.
It shows the current mode and operation step,
pipetting
volume, battery indicator and piston
status (aspirated and dispensed volume) in real
time.
Warning messages appear in the place of volume.
PIPETMAN M is provided with a minimal battery charge. Before using your new
PIPETMAN M, we strongly recommend you to fully charge the battery. PIPETMAN M charges
80 % of its full battery capacity in less than an hour, it takes three hours to fully charge the battery.
Please refer to PIPETMANM Power Management (Chapter 12) for charging procedure.
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• Press the push-button to aspirate the selected volume.
• To dispense:
Click (press and release) the push-button. Three things happen: sample
dispense, automatic purge and piston reset to zero.
or
Keep your thumb pressing the push-button until the end of the dispense
cycle: The piston reset takes place after releasing the push-button again.
This feature enables you to remove the tip from the liquid, without
aspir
ating anything.
• Eject tip by pressing the ejection button.
Your PIPETMAN M is ready again for next pipetting cycle while maintaining
the volume and the last settings!
Continue pipetting as you would with your mechanical PIPETMAN;
it’s as simple as that to use your new, electronic pipette!
REPE
TITIVE Mode
The REPETITIVE Mode allows you to distribute the same volume repeatedly in predefined number (N) of equal aliquots. You supply the aliquot volume (AV) and the
pipett
e automatically calculates the number of aliquots possible from the nominal
(maximum) volume (NV) of the pipette as follows:
N = NV/AV (e.g. 120 L x 10 for P1200M).
You also can decrease the number of repetitions (e.g. 120 L x 10-(n ≥1) for P1200M).
Application note: The REPETITIVE Mode is ideal for dispensing aliquots, e.g.; dispensing a PCR master mix into PCR
tubes or 96 plates, pipetting elution buer for DNA extraction, preparing a target for spectrometric analyses, distributing
loading buer into samples, etc.
•
Press Mode-button to access system menu, where you can choose between
different pipette modes.
Select REPETITIVE Mode by turning the push-button. To confirm your selection click on the push-button.
• Turn the push-button half way, the screen will display: “click to change vol.”
• Press the push-button; the volume on the display will start to blink, you can now
adjust the volume:
• Set the aliquot volume: click the push-button, set the volume by turning the push-button and click
again to confirm your settings.
• Set the aliquot number: The maximal number of aliquots has been calculated by your PIPETMAN
M. The number of aliquots flashes and you can decrease this number (N – (n ≥1)) by turning the
push-button. Click again to confirm your settings.
You are ready to pipette!
1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN TIPS for assurance of
accurate and precise results; refer to chapter 8).
5 PIPETTING JUST LIKE WITH PIPETMAN
Using PIPETMAN M is as easy as using a mechanical PIPETMAN. Your PIPETMAN M
is set by default on “PIPET Mode”, that means forward pipetting. You can select a
volume and start to pipette.
Switch on
Press push-button.
Adjust the volume setting
• Hold your PIPETMAN M in pipette position.
• Turn the push-button half way, the screen will display: “click to change vol.”
• Now press the push-button; the volume on the display will start blinking, you can now adjust the
volume:
• Turn the push-button either clockwise to decrease volume or counter clockwise to increase volume,
like indicated on the button.
• Press the push-button one time when finished. The volume is locked.
You are ready to pipette!
6 PIPETTING MODES
PIPETMAN M offers more pipette modes for a large number of applications: you will find all pipetting
modes in the menu (for system settings refer to the Chapters 7 and 13). Access the menu by pushing
the Mode-button. To choose one of the pipetting modes, turn the push-button and confirm your
selection with a simple click!
PIPET Mode
This is the classic pipette mode (forward pipetting), your pipette is simply aspirating
and dispensing.
Application note: You can use the PIPET Mode for all standard applications like DNA extraction, Plasmid Isolation,
Cloning, Dilution, PCR, qPCR and many others. You can easily pipette aqueous liquids like buer, chemical solutions
(MgCl2, KCl …) and biological samples like blood, DNA and RNA.
• Fit a tip suitable for the model of PIPETMAN M that you are
using (preferably use PIPETMAN TIPS for assurance of accurate
and precise results; refer to Chapter 8).
START button
Optional STOP
0
V
Purge
You must always t a tip
before using any pipette!
The tip can be emptied at any time during a pipetting cycle. To do so, turn
the push-button quickly: the message “click to abort” will appear on the
screen. Click on the push-button to validate: the liquid will be dispensed
and an automatic purge followed by piston reset to zero will occur.
You must always t a tip
before using any pipette!
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2. Click push-button to aspirate total
volume. The volume aspirated will be
a little bit more than required (extra
volume). The extra volume is necessary
to ensure equal operating conditions
for each dispensed aliquot.
3. To dispense: click the push-button.
DISCARD appears on the screen. Discard the extra volume and continue
dispensing the aliquots
. PIPETMAN
M distributes the aliquot volume each
time you click the push-button. The
number of aliquots left to dispense is
displayed on the screen.
A warning beep indicates the last aliquot!
4. To purge: Press the push-button, PURGE appears on the screen, click again to purge and reset the
piston. Keep your thumb pressing the push-button until the end of the purge: the piston reset
takes place after you release the push-button again. This feature enables you to remove the tip
from the liquid, without aspirating anything.
5. Eject tip by pressing the ejection button.
MIX Mode
This is the classic pipette mode (forward pipetting) followed by a mixing phase,
composed of repeatedly aspirating and dispensing, as well as an optional forward
pipetting step.
Application note: The Mode MIX can be used to prepare a PCR master mix, enzyme restriction mix, protein solution, oligo-
nucleotide dilution and to mix samples with gel loading buer and so on…you can mix two dierent solutions or homogenize
one solution. If you work with higher viscosity than water (e.g. restriction enzyme) you may change the aspiration speed
(refer to chapter 7). If you work with genomic DNA you should always pipette very carefully to avoid shearing and nicking.
• Press Mode-button to access menu, where you can choose between different pipette modes. Select
MIX Mode by turning the push-button, to confirm your selection click on the push-button.
• Set the first volume, named VOLUME 1: click the push-button, set the volume by turning the pushbutton and click to confirm your selection. This is the first volume you will aspirate and dispense.
Set the second volume, named VOLUME 2: click the push-button, you can now set the second
volume by turning the push-button. Click again to confirm your settings.
If you set VOLUME 1 or VOLUME 2= 0 µL, the step will not appear. If you set VOLUME 1 and 2 = 0 µL the cycle will
start directly with the MIX step.
• Set the MIX volume: click the push-button, set the volume by turning the push-button. Confirm
your settings with a simple click. This is the volume which will be aspirated and dispensed repeatedly. “MIX” as long as you press the push-button.
• Set the additional pipetting volume, named VOLUME 3: You can choose a volume to be aspirated
and dispensed after the mixing step, so you can continue routine pipetting.
START button Optional STOP
V (+ extra vol)*
Purge
Start Purge
V (vol)
0
DISCARD
ALIQUOTES
PURGE
Click to Purge
Discard
Click the push-button, set the volume by turning the push-button. Confirm your settings by a
simple click.
If you set the VOLUME 3 = 0 µL the cycle will stop after the MIX step.
You are ready to mix!
1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN Tips for assurance of
accurate and precise results; refer to chapter 8).
2. To aspirate VOLUME 1 press the push-button
3. To dispense:
Click (press and release) the push-button: Three things happen: sample dispense, automatic
purge and piston reset to zero
or
Keep your thumb pressing the push-button until the end of the dispense cycle: The piston reset
takes place after you release the push-button again. This feature enables you to remove the tip
from the liquid, without aspirating anything.
4. To aspirate and dispense the second volume “VOLUME 2”, repeat steps 2 and 3.
5. To mix: Press the push-button; as long as you keep your thumb pressing the button, the pipette
continues mixing. Release the push-button to complete the current mixing cycle.
6. To purge: press push-button, PURGE appears on the screen, click again to purge and reset the
piston.
7. To pipette: If you have chosen a VOLUME 3 > 0 you can now aspirate and dispense this volume by
repeating steps 2 and 3.
If you choose VOLUME1 = 0, step 2 won’t occur, if you choose VOLUME 2 = 0 µL, step 4 won’t occur, as well if you set
VOLUME 1 and 2 = 0 µL, step 1-4 will disappear. If you set VOLUME 3 = 0 µL step 7 will not occur.
You must always t a tip
before using any pipette!
START button Optional STOP
0
V
Purge
Start Purge
Push-button pressed
V1
V2
V3
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REVERSE Mode
The REVERSE Mode is reverse pipetting with a manual pipette. During aspiration,
additional liquid is added. After delivery, the excess volume remains in the tip and
is discarded.
Application note: The REVERSE Mode is ideal for pipetting viscous liquids; liquids with high vapour pressure or those
which tend to foam. Your PIPETMAN M aspirates a selected volume and an excess. This excess compensates for the liquid
that remains as a lm inside the tip during dispensing. For example: protein extraction, cell lysis, plasmid isolation, cell
culture, buer preparation and many others…
Electrophoresis gels loading protocol: rst change the pipetting speed of your pipette (see chapter 7). Aspiration can
be done by using the standard speed (by default speed 6). Dispensing should be done very slowly and carefully, to prevent
swirling and spilling of the samples, so select the lowest speed 1. Your pipette is now ready to load your gel! Please be
aware that if you purge without pulling out the tip air bubbles could get into your gel!
• Press Mode-button to access system menu, where you can choose between different pipette modes.
Select REVERSE Mode by turning the push-button, to confirm your selection click the push-button.
• Set the volume: click the push-button, set the volume by turning the push-button and click again
to confirm your settings.
You are ready to do reverse pipetting!
1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN TIPS for assurance
of accurate and precise results; refer to Chapter 8).
2. Click push-button to aspirate the selected volume. An
amount of liquid equal to the amount of purged air is
added, the volume aspirated will be a little bit more
than the set volume.
3. Click push-button to dispense the volume, the addition
-
ally amount of liquid remains in the tip.
4.
To purge: press push-button, PURGE appears on the
screen, click again to purge and reset the piston. Keep
your thumb pressing the push-button until the end
of the purge: the piston reset takes place after you
release the push-button again. This feature enables you
to remove the tip from the liquid, without aspirating
anything.
5. Eject tip by pressing the ejection button.
CUSTOM Mode
The CUSTOM Mode enables personalized pipetting protocol creation on a computer for transfer
to PIPETMAN M through PIPETMAN M programmable Software, an easy-to-use software.
T
o use this mode, the software has to be installed on the computer (file downloadable on
www.gilson.com/pipetmanm).
To install PIPETMAN M programmable software
1. Download the appropriate PIPETMAN M programmable software installation file for your
operating system to the computer.
2.
Double-click the installer and follow the instructions on the screen to complete the installation.
3. When completed, the PIPETMAN M software icon will appear on desktop of the computer if Create
Desktop Shortcut is checked.
4. If PIPETMAN M software is not launched automatically double click the PIPETMAN M icon on the
Desktop.
5. Once installed, the PIPETMAN M software can be run by any user on the installed computer.
You can then create, edit, import or export custom protocols easily and rapidly.
To create a protocol, choose among the available tasks in PIPETMAN M programmable software:
• Aspirate: aspirates specified volume into the tip
• Dispense: dispenses specified volume from the tip
• Dispense all: dispenses all remaining volume from the tip followed by a purge action
• Repeat: enables repetition of any other tasks once or more
• Mix: mixes the specific volume in one or more cycles
• Purge: dispenses all remaining volume from the tip followed by purging extra volume
• Wait: introduces a wait for a specified number of seconds
• Wait for click: pauses the protocol until Push-button is clicked
• Beep: makes pipette produce a beep sound while selected tasks are executed
In this mode, the protocols can be automatically executed, avoiding repetitive pushes on the button,
helping to decrease risk or RSI.
To transfer a protocol to your PIPETMAN M pipette, click the “Transfer Custom Protocols to Pipette”
button and follow the instructions on the screen.
The custom mode is available in pipettes from firmware version V2.02.
For more information on PIPETMAN M software, see p34.
0
Purge
START button
Optional STOP
Start Purge
V (+ extra vol)*
Click to Purge
You must always t a tip before
using any pipette!
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7 PIPETTING SPEED CONTROL
PIPETMAN M is set by default on speed 6 (maximum speed). You may need to change the speed of
aspiration or dispensing depending on your application. You can change aspiration speed and dispensing speed independently of one other: from very slow to very fast (speed 1 – speed 6). Your speed
selection is memorized for each pipetting mode until you change it again.
• Press Mode-button to access system menu, where you can find the different pipette modes, the
speed menu and configuration menu.
• Select SPEED by turning the push-button, to confirm your selection click on the push-button.
• Set ASPIRATION SPEED: aspiration speed is blinking, set the speed by turning the push-button and
click to confirm your selection.
• Set DISPENSING SPEED: dispensing speed is blinking, set the speed by turning the push-button
and click to confirm your selection.
• After selecting the speed you go automatically back to pipetting mode.
8 PIPETMAN TIPS
PIPETMAN TIPS are made to the highest specifications; strict quality control
is maintained throughout the manufacturing process. These tips are used to
calibrate PIPETMAN M, therefore for optimum performance we recommend
using PIPETMAN TIPS with your PIPETMAN M.
However, PIPETMAN M also offers you a high compatibility with a large
number of other tips. For more information please feel free to contact your
Gilson Distributor.
PIPETMAN TIPS have the Gilson logo engraved on their collar, ensuring that
you have a genuine Gilson product. To ensure accuracy and precision, Gilson’s Quality Assurance System focuses on the following critical parameters.
• PIPETMAN TIPS are made from pure polypropylene (virgin, metal-free, to avoid the possibility of
contamination). They are available sterilized and with filters.
• Sterilized PIPETMAN TIPS are certified free of detectable RNases, DNases, DNA, RNA, and proteases.
• Optimized shape (revised collar for optimum sealing, thin walls, and fine point), making them easier
to mount, more flexible, with no vortexing, and improved precision.
• PIPETMAN TIPS are free from even microscopic defects, especially at the orifice. All surfaces are
smooth and hydrophobic, thereby avoiding the excessive retention of liquids that causes poor ac
-
curacy and a lack of precision.
•
Mold and cavity references are marked on the collar, ensuring the traceability. For quality assurance
purposes batch numbers appear on all packages (bags and boxes).
• They form an air-tight seal with the tip-holder, preventing the leaks that cause poor accuracy and a
lack of precision.
Gilson logo ID Number
• PIPETMAN TIPS (except filter tips) may be autoclaved at 121°C for 20 minutes at 0.1 MPa.
Do not autoclave PIPETMAN Filter Tips,
the lter will be damaged.
To ensure the best performance from
your Gilson pipette, you shoud ALWAYS
use PIPETMAN Tips (in accordance with
ISO8655) system, because PIPETMAN
Tips were used to establish the
specications.
*
A plastic adapter is required to eject D10 and DF10 tips (short
tips). The adapter is supplied with P10M. No adapter is required
when using DL10 and DFL10 tips (long tips).
Model Tips model
Range
Color code
(PIPET mode)
P8x10M D10* - DL10
0.5 µL to 10 µL Red
P12x10M DF10* - DFL10
P8x20M DL10 - D200
1 µL to 20 µL Pale yellow
P12x20M DFL10 - DF30
P8x100M D200
10 µL to 100 µL Salmon
P12x100M DF100
P8x200M D200 - D300
20 µL to 200 µL Yellow
P12x200M
DF100 - DF200 - DF300
P8x300M D200 - D300
10 µL to 300 µL Green
P12x300M DF200 - DF300
P8x1200M D1200
50 µL to 1200 µL Blue
P12x1200M DF1200
Model Tips model Range Color code
(PIPET mode)
P10M
D10* - DL10
0.5 µL to 10 µL Red
DF10* - DFL10
P20M
D200
2 µL to 20 µL Pale yellow
DF30
P100M
D200
5 µL to 100 µL Salmon
DF100
P200M
D200 - D300
20 µL to 200 µL Yellow
DF200 - DF300
P300M
D200 - D300
20 µL to 300 µL Green
DF200 - DF300
P1200M
D1000 - D1200
100 µL to 1200 µL Blue
DF1000 - DF1200
P5000M D5000 500 µL to 5000 µL Purple
P10mLM D10 mL 1 µL to 10 mL Pale blue
Ejector spacer D10
Ejector spacer
Short tips
Long tips
Short tips Long tips
Plastic adapter P8x10M and P12x10M
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9 GUIDELINES FOR GOOD PIPETTING
Aspirate and dispense
1) Fit new PIPETMAN Tip for the best results (see Chapter 18).
For single channel models, push the tip-holder into the tip
using a slight twisting motion to ensure a firm and airtight
seal.
For multichannel models,PIPETMAN Tips are best fitted from
the patented ROCKY RACK available only in our TIPACKs and
TOWERPACKs. ROCKY RACK is the dome-shaped part of the
pack that contains the tips. ROCKY RACK makes it easy to
securely fit the tips to a multichannel pipette, ensuring an
airtight seal on all channels without the need to use undue
pressure or to touch the tips.
2) Pre-rinse the tip. Pre-rinsing consists of aspirating the first
volume of liquid and then dispensing it back into the same
vessel (or to waste). Subsequent volumes that you pipette
will have levels of accuracy and precision within specifications. Some liquids (e.g. protein-containing solutions and
or
ganic solvents) can leave a film of liquid on the inside the
wall of the tip; pre-rinsing the tip minimizes any errors that
may be related to this phenomenon.
3) Hold the pipette vertically and immerse the tip in the liquid
(see page 13). Press the push-button to aspirate the set volume of liquid. Wait a few seconds (time depends on model,
see page 13); then withdr
aw the pipette-tip from the liquid.
You may wipe any droplets away from the outside of the
tip using a medical wipe, however if you do so take care to
avoid touching the tip’s orifice.
4) Place the end of the tip against the inside wall of the recipient
vessel (at an angle of 10° to 40°). Press the push-button. Wait
for at least a few seconds before releasing the push-button
to expel any residual liquid from the tip. While removing the
pipette draw the tip along the inside surface of the vessel.
5) Eject the tip by pressing firmly on the tip-ejector button.
General guidelines for good pipetting
a. Make sure that you fit new tips.
b. Each new tip should be pre-rinsed with the liquid to be pipetted.
c. When aspirating, keep the tip at a constant depth below the
surface of the liquid (refer to Table).
Immersion Depth and Wait Time
Model Immersion Depth Wait Time
(mm) (seconds)
P10M 1 1
P20M 2-3 1
P100M 2-4 1
P200M 2-4 1
P300M 2-4 1
P1200M 2-4 2-3
P5000M 3-6 4-5
P10 mLM 5-7 4-5
P8x10M, P12x10M 1 1
P8x20M, P12x20M 2-3 1
P8x100M, P12x100M 2-4 1
P8x200M, P12x200M 2-3 1
P8x300M, P12x300M 2-3 1
P8x1200M, P12x1200M 2-4 2-3
d. Change the tip before aspirating a different liquid, sample, or reagent.
e. Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
f. Liquid should never enter the tip holder. To prevent this:
• Never turn the pipette upside down,
• Never lay the pipette on its side when there is liquid in the tip(s),
g. The Gilson Stand Adapter (see Chapter 2) is recommended for use with the CARROUSEL and SINGLE
Pipette Holder to store your PIPETMAN M pipette in the vertical position. Alternatively the POWER
CARROUSEL can be used to store and charge up to 5 PIPETMAN M pipettes.
h. When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip
several times before use in order to reach equilibrium between the temperatures of the liquid and
the pipette’s dead-volume.
i. For volatile liquids you should saturate the dead-volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
j. After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as described in
Chapter 15.
k. The pipette can be used between + 4 °C and + 40 °C, but the
specifications may vary (see Chapter 18).
l. Do not pipette liquids having temperatures above 50 °C or
below 4 °C.
10 PERSONALIZE YOUR PIPETMAN M
Tip-ejector extension for use with P10M
In order to eject D10 tips, tip-ejector extensions are supplied with P10M
pipettes.
The tip-ejector extension which is made of PVDF (polyvinylidene Fluoride),
is autoclavable.
To t a tip-ejector extension:
• Hold the pipette with the push-button upright.
• Hold the extension with the slot upright.
• Slide the extension over the tip-holder.
• Push the extension firmly onto the end of the tip-ejector until it clicks
into place (see opposite).
To remove a tip-ejector extension (see opposite):
• Hold the pipette in one hand and grip the extension with the other.
• Gently twist the extension (either direction) and pull it away from the
pipette.
Short tips Long tips
To fit To remove
Slot
Extreme temperatures
can aect accuracy and
precision!
- 17 -- 16 -
USER’S GUIDE
EN
PIPETMAN M
USER’S GUIDE
EN
PIPETMAN M
Personal label – Name and/or application tag:
Your PIPETMAN M offers you two options to mark your pipette. You can
personalize your pipette with a name tag and/or an application tag:
Window-tag:
•
Remove the window by inserting a small screwdriver in the access slot.
•
Position the name tag on the pipette
•
Clip the window back in place.
Clip-tag:
•
Pull off the clip.
•
Position the name tag into the clip
•
Clamp the clip back in place.
11 GOOD LABORATORY PRACTICE FEATURES
Your PIPETMAN M is fully compliant to ISO8655 standard and is CE marked (for IVD and EMC directives).
PIPETMAN M includes the following GLP features.
General
• Lockable volume.
• Useful volume-range is printed on the push-button.
• Volume range is displayed on the screen.
• Serial number is engraved on the body (handle) of the pipette and encoded in the firmware.
• Bar code: on the box and with the certificate (can be transferred).
• Personalization by nametags (for marking application and/or user name), see Chapter 10.
Cycle counters
• From last volume setting (to count the number of cycles in the current “run”).
• From manufacture and since the last service (see Chapter 14).
• By indicators (R1, R2, R3 … Rx) each time pipette is readjusted.
• Maintenance intervals can be specified by weeks or number of cycles elapsed.
Alarms
• Low battery warning.
• Service is overdue warning (see Chapter 14).
• Service Alert can be set “On” or “Off”.
Window-tag
Clip-tag
12 PIPETMAN M POWER MANAGEMENT
The PIPE TMAN M battery has been designed to
ensure 900 pipetting cycles (750 for PIPETMAN
M Multi 200 µL and 300 µL) at maximum speed
(up to nine 96 well plates) between two charge
cycles. For service continuity when needing more
capacity, your PIPETMAN M can also be used while
charging with the same performance as a fully
charged pipette!
Battery charging
PIPETMAN M charges 80 % of its full battery capacity in less than an hour, it takes three hours to fully
char
ge the battery.
Charge your pipette by using the power supply:
- Connect the AC adapter to a suitable AC
power supply
- Plug the adapter into the USB port of the
pipette.
- The pipette starts charging.
Charge your pipette by using stand adapter
(see Chapter 2):
- First place the stand adapter on your Gilson
SINGLE pipette holder or CARROUSEL.
- Remove the plug. Now place your pipette
on the stand adapter and plug the power
transformer into the stand adapter.
- The pipette starts charging.
Battery charging of PIPETMAN M using the
POWER CARROUSEL:
- First install the POWER CARROUSEL as
described above.
-
Remove the plug. Now place your pipette
on the charging position, please ensure that
the pipette is properly fitted to the contacts
ont the top of the carrousel (a proper fitting
will trigger a beep sound).
- The pipette starts charging.
Stand adapter
Plug
USB port
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