Gilson MyPipetman User Manual

Page 1
USER’S GUIDE
EN
Page 2
TABLE OF CONTENTS
TABLE OF CONTENTS
INTRODUCTION | 4
Parts Checklist | 4
GLP Features | 4
Description | 5
Specifications | 6
Simple Ratchet | 7
3-Position Locking System | 7
Secure Pipetting | 7
PIPETTING | 8
Fitting the Tip Ejector Adapter | 8
Fitting the Tips | 9
Pre-Rinse the Tips | 9
Aspirate | 10
Dispense | 10
Guidelines for Good Pipetting | 11
ACCESSORIES | 12
TROUBLESHOOTING | 13
Troubleshooting Table | 13
Leak Test | 14
CLEANING AND DECONTAMINATION | 14
Autoclaving | 14
Chemical Decontamination | 14
Lower Part (Volumetric Module) | 15
MAINTENANCE | 16
Changing the Tip Ejector for Single Channel | 16
Changing the Tip Holder for Single Channel | 16
Changing the Tip Ejector for Multichannel | 16
Servicing the Piston Assembly | 17
SPARE PARTS | 18
WARRANTY | 19
TABLE OF CONTENTS | MyPIPETMAN®
3
| MyPIPETMAN®
Page 3
Chapter 1
INTRODUCTION
Your personally designed MyPIPETMAN® pipette has an air-displacement mechanism and is used with disposable pipette tips.
This Gilson pipette line provides:
Light and comfortable body
Unique design and configuration
Low pipetting forces ensuring ergonomics for users’ well-being
Eight single channel models cover a volume range from 0.2 μL to 10 mL.
Eight multichannel models (4 models of 8 and 12 multichannel) cover a volume range from 0.5 μL to 300 μL.
Parts Checklist
Carefully unpack your pipette and take a moment to verify that the following items are included and undamaged:
SINGLE MODELS
Your personalized MyPIPETMAN
Quick Guide
Certificate of Conformity
Safety bag
Tip ejector adapter equipped on the P2 μL and P10 μL models (fully autoclavable)
Bag of ten filters channel models
(only for P10mL
)
single
MULTICHANNEL MODELS
Your personalized MyPIPETMAN
Quick Guide
Ejector spacer for short collar tips (only for P10 μL models)
Certificate of Conformity
Safety bag
INTRODUCTION
GLP Features
The serial number is engraved on the body of the pipette. It provides unique identification of your pipette and the date of manufacture. Example: MY524CQ
The barcode on the box and the Certificate of Conformity provide traceability of your pipette.
RANDOM NUMBER
231
354
324
522
457
120
876
489
542
MONTH CODE
January A
February B
March C
April D
May E
June G
July H
August J
September K
October L
November M
December N
YEAR CODE
2019 Q
2020 R
2021 S
2022 T
2023 U
2024 W
2025 X
2026 Y
2027 Z
MY 524 C Q
MODEL
RANDOM
MONTH YEAR
NUMBER
MYPIPETMAN 524 MARCH 2019
Figure 1
Serial number
MyPIPETMAN® | USER’S GUIDE
4
Page 4
Description
Please refer to the following chapters for a full description of the different parts and functions of the pipette.
INTRODUCTION
Color window
Single channel
Multichannel
Color-coded
push button
Locking system
Tip ejector
button
Personalized
handle
Color ring
Figure 2
MYPIPETMAN™ single channel and multichannel models
Connecting nut
Ejector clip
Tip holder
Ejector lock
Cover
Tip ejector
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INTRODUCTION | MyPIPETMAN®
Page 5
Specifications
NOTE
MyPIPETMAN is a high-quality pipette that offers excellent accuracy and precision especially with PIPETMAN DIAMOND Tips. The data given in the “Gilson Maximum Permissible Errors” table were obtained using PIPETMAN DIAMOND Tips. These values are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.
Each pipette is inspected and validated by qualified technicians in accordance with the Gilson quality system. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard by type testing.
The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6).
INTRODUCTION
MODEL VOLUME (µL)
MyPIPETMAN SINGLE CHANNEL
0.2
MyPIPETMAN P2
MyPIPETMAN P10
MyPIPETMAN P20
MyPIPETMAN P100
MyPIPETMAN P200
MyPIPETMAN P1000
0.5 1 2
1 5 10
2 10 20
10 50 100
20 100 200
100 500 1000
GILSON MAXIMUM PERMISSIBLE ERROR
Systematic Error (µL)
± 0.024 ± 0.025 ± 0.027 ± 0.030
± 0.025 ± 0.075 ± 0.100
± 0.10 ± 0.10 ± 0.20
± 0.35 ± 0.40 ± 0.80
± 0.50 ± 0.80 ± 1.60
± 3 ± 4 ± 8
Random Error (µL)
0.012 0.012 0.013 0.014
0.012 0.030 0.040
0.03 0.05 0.06
0.10 0.12 0.15
0.20 0.25 0.30
0.6 1.0 1.5
ISO-8655 MAXIMUM PERMISSIBLE ERROR
Systematic Error (µL)
± 0.08 ± 0.08 ± 0.08 ± 0.08
± 0.12 ± 0.12 ± 0.12
± 0.2 ± 0.2 ± 0.2
± 0.8 ± 0.8 ± 0.8
± 1.6 ± 1.6 ± 1.6
± 8.0 ± 8.0 ± 8.0
Random Error (µL)
0.04 0.04 0.04 0.04
0.08 0.08 0.08
0.1 0.1 0.1
0.3 0.3 0.3
0.6 0.6 0.6
3.0 3.0 3.0
MyPIPETMAN P5000
MyPIPETMAN P10mL
MyPIPETMAN MULTICHANNEL
MyPIPETMAN P8x10
MyPIPETMAN P12x10
MyPIPETMAN P8x20
MyPIPETMAN P12x20
MyPIPETMAN P8x200
MyPIPETMAN P12x200
MyPIPETMAN P8x300
MyPIPETMAN P12x300
500 2500 5000
1000 5000 10000
0.5 1 5 10
2 10 20
20 100 200
20 30 150 300
± 12 ± 15 ± 30
± 30 ± 40 ± 60
± 0.08 ± 0.08 ± 0.20 ± 0.20
± 0.10 ± 0.20 ± 0.40
± 0.50 ± 1.00 ± 2.00
± 1.00 ± 1.00 ± 1.50 ± 3.00
3 5 8
6 10 16
0.04 0.05 0.10 0.10
0.08 0.10 0.15
0.25 0.40 0.50
0.35 0.35 0.60 1.00
± 40 ± 40 ± 40
± 60 ± 60 ± 60
± 0.24 ± 0.24 ± 0.24 ± 0.24
± 0.4 ± 0.4 ± 0.4
± 3.2 ± 3.2 ± 3.2
± 8.0 ± 8.0 ± 8.0 ± 8.0
15.0 15.0 15.0
30.0 30.0 30.0
0.16 0.16 0.16 0.16
0.20 0.20 0.20
1.2 1.2 1.2
3.0 3.0 3.0 3.0
The data given in this table conform to the ISO 8655-2 standard. With a precise pipetting technique, the P2 model can be used to aspirate volumes as low as 0.1 μL and the P10 model as low as 0.5 μL.
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Chapter 2
NOTE
SETTING THE VOLUME
The volume of liquid to be aspirated is set using the volumeter. The dials are colored either black or red to indicate the position of the decimal point, depending on the model (refer to Figure 3 below).
Volume selection differs according to your configuration. To obtain maximum accuracy when setting the volume, proceed as follows:
When decreasing the volume setting, slowly reach the required setting, making sure not to overshoot the volume mark.
When increasing the volume setting, pass the required value by 1/3 of a turn and then slowly decrease to reach the volume, making sure not to overshoot the volume mark.
SETTING THE VOLUME
MODEL
MyPIPETMAN SINGLE CHANNEL
MyPIPETMAN P2 µL 0.01 µL 0.002 µL
MyPIPETMAN P10 µL 0.1 µL 0.02 µL
MyPIPETMAN P20 µL 0.1 µL 0.02 µL
MyPIPETMAN P100 µL - 0.2 µL
MyPIPETMAN P200 µL - 0.2 µL
MyPIPETMAN P1000 0.01 mL mL 0.002 mL
MyPIPETMAN P5000 0.01 mL mL 0.002 mL
MyPIPETMAN P10mL mL 0.1 mL 0.02 mL
MyPIPETMAN MULTICHANNEL
MyPIPETMAN P8x10 µL 0.1 µL 0.02 µL
MyPIPETMAN P12x10 µL 0.1 µL 0.02 µL
MyPIPETMAN P8x200 µL - 0.2 µL
MyPIPETMAN P12x200 µL - 0.2 µL
MyPIPETMAN P8x300 µL - 0.2 µL
MyPIPETMAN P12x300 µL - 0.2 µL
COLOR OF VOLUMETER NUMBERS
BLACK RED INCREMENT
P2
1
2 5
1.25 µL
P200
1 2 5
125 µL
Figure 3
Dial colors by model
Simple Ratchet
For pipettes mounted with a simple ratchet, select the volume by turning the push button (refer to Figure 4).
3-Position Locking System
For pipettes equipped with a 3-position locking system, turn the selector to your desired position (refer to Figure 5).
Single channel
model
P10
7.5 µL
P1000
P20
0 7
5
1 2
5
12.5 µL
P5000
0
7 5
0.75 mL
1.25 mL
Multichannel
model
P100
x10
0 7 5
75 µL
1
2 5
P10mL
0 7
5
7.5 mL
7.5 µL
x200
125 µL
x20
0 7
5
1 2
5
12.5 µL
1 2 5
x300
1 2 5
125 µL
Free volume selection position
1. Turn the selector to the left until the cursor reaches the unlocking icon 1.
2. Select the volume by turning the push button.
Light click volume selection position
1. Turn the selector until the cursor reaches the middle icon 2.
2. Select the volume by turning the push button.
When selecting the volume, you will hear click sounds, allowing for more precise settings. Use these sounds for final fine volume selection.
Secure Pipetting
After selecting your volume, turn the selector to the right until the cursor reaches the locking icon for secure pipetting 3.
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SETTING THE VOLUME | MyPIPETMAN®
Figure 4
Simple ratchet
1
Figure 5
3-position locking system
2
3
Page 7
Chapter 3
NOTE
PIPETTING
Fitting the Tip Ejector Adapter
For P2 and P10 pipettes equipped with a stainless steel tip ejector, a dual-position adapter is required to fit and eject DL10 tips (long tips) and D10 tips (short tips).
To fit the adapter for short and long tips (refer to Figure 6):
1. Pull the adapter down from the stainless steel tip ejector.
2. Turn the adapter 180° 2.
3. Refit the adapter so that the end of the stainless steel tip ejector engages the shorter or longer slot of the adapter 1.
For P2 and P10 pipettes equipped with a plastic tip ejector, a dual-position adapter (refer to Figure 7) is required to fit and eject D10 tips (short tips).
To fit a tip ejector adapter:
1. Slide the adapter over the tip holder 1.
2. Push the adapter firmly onto the end of the tip ejector until it clicks into place.
To remove a tip ejector adapter:
1. Gently twist the adapter 2.
2. Pull it away from the pipette 3.
Fitting the Ejector Adapter for P8x10 and P12x10 Multichannel Models
According to the tip used, D10 or DL10, you might have to fit the ejector adapter; long collar tips can be inserted without it.
1. Remove the tip ejector, keep both ejector locks depressed 1, and then pull the tip ejector down 2.
2. Fit the ejector adapter 3 and click it to the tip ejector.
3. To refit the tip ejector, gently re-insert the tip ejector vertically into the rails of the ejector support 4.
Figure 6
Adapter for short and long tips
Figure 7
Dual-position adapter
Short tips
1
Long tips
2
3
2
PIPETTING
1
Long tips can be used without tip ejector adapter.
1
2
3
4
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PIPETTING
Fitting the Tips
It is recommended to use PIPETMAN® DIAMOND Tips with MyPIPETMAN for optimum performance. These tips are made from pure polypropylene. Plastic tips are for a single application—they should not be cleaned for reuse.
Single Channel Models
To fit a new PIPETMAN DIAMOND Tip, push the tip holder into the tip using a slight twisting motion to ensure a firm and airtight seal.
PIPETMAN DIAMOND Tip Compatibility
for Single Channel Models
P2, P10 D10, DL10, DF10, DFL10
P20 D200, DF30
P100 D200, DF100
P200 D200, DF200, D300, DF300
P1000 D1000, D1200, DF1000, DF1200
P5000 D5000
P10mL D10mL
Multichannel Models
PIPETMAN DIAMOND Tips are best fitted onto multichannel pipettes from the patented ROCKY RACK available only in our TI PACK S and
TOWERPACKS.
PIPETMAN DIAMOND Tip Compatibility
for Multichannel Models
P8x10L, P12x10L D10*, DL10, DF10, DFL10
P8x20L, P12x20L DL10, DFL10, DF30, D200
P8x200L, P12x200L D200, D300, DF200, DF300
P8x300L, P12x300L D200, D300, DF200, DF300
*Using an ejector adapter, you can adapt a D10 tip (refer to page 8)
Figure 8
The patented ROCK Y RACK system available on TOWERPACK and TIPACK makes it extremely easy to fit tips on a multichannel pipette
Pre-Rinse the Tips
Some liquids (e.g., protein-containing solutions and organic solvents) can leave a film of liquid on the inside wall of the tip. Pre-rinse the tip to minimize any errors that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it to waste.
Tips will not fall off nor will they have to be manually positioned. Subsequent volumes that you pipette will have levels of accuracy and precision within specifications.
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PIPETTING | MyPIPETMAN®
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Aspirate
NOTE
1. Press the push button to the first stop (this corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the tip in the liquid (refer to Guidelines for Good Pipetting on page 11.
3. Release the push button slowly and smoothly (to top position) to aspirate the set volume of liquid.
4. Wait one second (refer to Guidelines for Good Pipetting on page 11) and then withdraw the pipette tip from the liquid. You may wipe any droplets away from the outside of the tip using a medical wipe; however, if you do so take care to avoid touching the tip’s orifice.
For the multichannel models, use a reagent reservoir.
Dispense
1. Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly to the first stop.
3. Wait for at least a second, then press the push button to the second stop to expel any residual liquid from the tip. Keep the push button pressed fully down and (while removing the pipette) draw the tip along the inside surface of the vessel.
4. Release the push button smoothly. Eject the tip by pressing firmly on the tip ejector button.
PIPETTING
Rest position
First stop
Second stop
Prepare
Aspirate Dispense
Purge Home
Figure 9
Pipetting motion - aspirate and dispense
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Page 10
Guidelines for Good Pipetting
Make sure that you operate the push button slowly and smoothly.
When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table).
PIPETTING
MODEL
P2, P10, P8x10, P12x10 1 1
P20, P8x20, P12x20, P8x200, P12x200 2-3 1
P8x300, P12x300 2-4 1
P1000 2-4 2-3
P5000 3-6 4-5
P10mL 5-7 4-5
Change the tip before aspirating a different liquid, sample, or reagent.
Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid to be pipetted.
Liquid should never enter the tip holder; to prevent this:
Press and release the push button slowly and smoothly.
Never turn the pipette upside down.
Never lay the pipette on its side when there is liquid in the tip.
If you use the same tip with a larger volume, pre-rinse the tip.
For volatile solvents, you should saturate the air cushion in your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample.
When the temperature of the liquid is different from the ambient temperature, pre-rinse the tip several times before use.
You may remove the tip ejector (refer to Fitting the Tip Ejector Adapter on page 8) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids that emit vapors, remove the tip ejector and tip holder, rinse with distilled water, dry, and lubricate the piston (refer to MAINTENANCE on page 16). For the P1000L model, use a specific tip holder equipped with a filter to increase the lifetime of the piston (refer to ACCESSORIES on page 12).
Do not pipette liquids with temperatures above 70°C or below 4°C. The pipette can be used between +4°C and +40°C, but the gravimetric performance may vary according to the temperature (refer to the ISO 8655-2 standard for conditions of use).
IMMERSION DEPTH (MILLIMETERS)
WAIT TI ME (SECONDS)
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PIPETTING | MyPIPETMAN®
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Chapter 4
ACCESSORIES
To make pipetting more comfortable and more secure, Gilson has developed several accessories.
Pipette stands allow users to store pipettes vertically to avoid the possibility of liquid running back into the pipette.
DESCRIPTION PART NUMBER
CARROUSEL Pipette Stand (7 pipettes) F161401
TRIO Pipette Stand (3 pipettes) F161405
Universal Multichannel Stand* F161417
SINGLE Pipette Holder F161406
* Universal Multichannel Stand is for multichannel models only.
COLORIS™ Identification Clips identify or personalize your pipette. Write your name or application to avoid mix-ups
and then snap into place.
DESCRIPTION PART NUMBER
COLORIS clips (mixed colors set of 10) F161301
COLORIS clips (red, set of 10) F161302
COLORIS clips (yellow, set of 10) F161303
COLORIS clips (green, set of 10) F161304
COLORIS clips (blue, set of 10) F161305
COLORIS clips (white, set of 10) F161306
Universal Multichannel
Stand
COLORIS™
Identification Clip
ACCESSORIES
CARROUSEL™
TRIO™
Pipette Stand
SINGLE™
Pipette Holder
With the hands-free JIMMY™ Microtube Opener, you can open both snap-cap and screw-cap microtubes to increase pipetting efficiency and reduce the fatigue associated with manual tube opening.
DESCRIPTION PART NUMBER
JIMMY Microtube Opener (set of 3) F144983
To protect the piston when pipetting corrosive liquids, you can use a specific tip holder and filter for the model P1000L.
DESCRIPTION PART NUMBER
Corrosion Protection Kit (tip holder + a bag of 10 filters) F144570
Corrosion Protection Kit Filter (Bag of 10) F144571
Corrosion Protection Kit Filter (Bag of 50) F144572
Disposable reagent reservoirs are ideal for dispensing reagent with multichannel pipettes.
DESCRIPTION PART NUMBER
Reagent reservoir 25 mL (x100) F267660
Reagent reservoir 50 mL (x100) F267670
Corrosion Protection Kit
Reagent Reservoir
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Chapter 5
NOTE
WARNING
TROUBLESHOOTING
A quick inspection of the pipette may help you to detect a potential problem.
You may download the Two Minute Inspection Poster from the Gilson website (www.gilson.com), which shows how to perform a quick diagnosis of your pipette.
Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of chemical, biological, or radioactive contamination. Refer to
CLEANING AND DECONTAMINATION on page 14. Please use the included safety bag
to return the pipette to your local Gilson Service Center.
Troubleshooting Table
The following table may help you to identify and correct the potential problem you might encounter.
TROUBLESHOOTING
For single channel and multichannel
SYM PTOM POSSIBLE CAUSE ACTION MODEL*
Pipette is leaking sample
Pipette won’t aspirate
Pipette is inaccurate
Pipette is not precise
Tips fall o or do not fit correctly
Pipette seizes up Piston needs lubricant Lubricate piston assembly S
Damaged tip holder Replace the tip holder S
Worn O-ring or seal Replace both parts and lubricate S
Worn O-ring Replace O-Ring and seal and lubricate S
Damaged tip holder Replace the tip holder S
Connecting nut is loose Tighten connecting nut S+M
Damaged or corroded piston
Improper repair or assembly Refer to page 16 - MAINTENANCE S
Improper repair or assembly Refer to page 16 - MAINTENANCE S
Unscrew tip holder Tighten connecting nut S
Connecting nut is loose Tighten connecting nut S+M
Tip holder is loose Tighten connecting nut S
Connecting nut is loose Tighten connecting nut S+M
Incorrect operator technique
Damaged or corroded piston(s)
Damaged tip holder(s) Replace the tip holder(s) S
Worn O-ring or seal Replace both parts and lubricate S
Low quality tips Use PIPETMAN DIAMOND Tips S+M
Damaged tip holder(s)
Damaged tip ejector adapter
Damaged ejector Replace the tip ejector S+M
Dirty tip holder
The tip ejector is loose Assemble the tip ejector properly S
The ejector lock is misaligned
Return pipette to supplier S
Operator training S+M
Return pipette to supplier S
Replace the tip holder(s) for single channel model Contact your local Gilson Service Center for multichannel model
Replace the tip ejector adapter S+M
Clean the tip holder with isopropanol or ethanol
Align the ejector lock S
S+M
S+M
*S = Sing le Chann el
M = Multichannel
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Page 13
Leak Test
NOTE
This test may be performed at any time to check that the pipette does not leak, especially after performing a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After making sure that the pipette is correctly reassembled, repeat this test.
1. Fit a PIPETMAN DIAMOND Tip.
2. For the variable volume models, set the pipette to the maximum volume given in the specifications, and pre-rinse.
For P2 to P200, pre-rinse the tip.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds.
If a water droplet appears at the end of the tip, there is a leak.
If you see no droplets, re-immerse the tip below the surface of water.
The water level inside the tip should remain constant; if the level goes down there is a leak.
no leak
Figure 10
MyPIPETMAN® multichannel leak test
Chapter 6
CLEANING AND DECONTAMINATION
MyPIPETMAN pipettes are fully autoclavable without disassembly for maximum convenience as well as protection from contamination.
CLEANING AND DECONTAMINATION
Autoclaving
1. Before autoclaving
Set the pipette volume to the lowest designated usable volume of the model before placing it in the autoclave.
2. Procedure
MyPIPETMAN can be sterilized by steam autoclaving at 121°C (252°F), 1 bar relative pressure for 20 minutes without disassembly.
When autoclaving, the pipette will dry better and faster without using a bag.
3. After autoclaving
Following the autoclaving cycle, let the pipette cool down to room temperature and dry completely before use.
Verify pipette’s functionality according to your procedure.
Chemical Decontamination
You may choose to decontaminate your pipette chemically, in accordance with your own procedures. Whatever decontaminant you use, check with the supplier to verify that the decontaminant is compatible with stainless steel and the plastics used in the construction of the pipette: PA (Polyamide), PBT (Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephtalate), POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), PP (Polypropylene), PPA (Polyphthalamide), or PPS (Polyphenylene Sulfide).
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CLEANING AND DECONTAMINATION
NOTE
Lower Part (Volumetric Module)
For Single Channel Models
The following components only can be immersed in a decontaminant solution: connecting nut, tip ejector, and tip holder.
The piston assembly and seals must be degreased with isopropanol or ethanol in a separate vessel before being immersed in decontamination solution.
1. Disassemble the pipette
2. Immerse tip ejector, tip holder, and connecting nut in the decontamination solution.
3. Disassemble and degrease the piston assembly (Refer to Servicing Piston Assembly, on page 17)
4. Rinse the connecting nut, tip ejector, and tip holder with distilled water.
5. Let the parts dry by evaporation (or wipe the tip ejector, the tip holder, and connecting nut with a soft cloth).
6. Lubricate the piston assembly and the seals (Refer to Servicing Piston Assembly, on page 17).
7. Reassemble the piston assembly, the tip holder, and the tip ejector.
For Multichannel Models
The following components only can be immersed in a decontamination solution: tip ejector, ejector locks, and ejector spacer.
1. Remove the tip ejector and the ejector adapter.
2. Immerse the tip ejector, ejector locks, and ejector adapter in the decontamination solution or wipe them with a soft cloth or lint-free tissue soaked with the decontamination solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the decontamination solution.
5. Wipe it with distilled water.
6. Let the parts dry by evaporation or wipe them with a clean soft cloth or lint-free tissue.
7. Refit the tip ejector (refer to Fitting the Tip Ejector Adapter on page 8).
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CLEANING AND DECONTAMINATION | MyPIPETMAN®
Page 15
Chapter 7
MAINTENANCE
Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance.
Visit the Gilson website at www.gilson.com for:
Complete technical service contact information
Access to the documentation and information about accessories and related products
For information or technical assistance contact techsupport@pipetman.com
MAINTENANCE
Changing the Tip Ejector for Single Channel
To remove
1. Push the ejection button 1.
2. Turn the tip ejector clockwise 2.
3. Slide and remove the tip ejector 3.
To refit
1. Push the ejection button.
2. Slide the tip ejector along the tip holder.
3. Clip the tip ejector on the body of the pipette.
Changing the Tip Holder for Single Channel
1. Push the ejection button.
2. Turn the tip ejector clockwise.
3. Slide and remove the tip ejector.
4. Unscrew the connecting nut.
Changing the Tip Ejector for Multichannel
To remove
1. Keep both ejector locks depressed 1.
2. Pull the tip ejector down 2.
1
2
3
To refit
1. Gently re-insert the tip ejector vertically into the rails of the ejector support 3.
2. Pull lightly on the tip ejector to check the position.
1
2
3
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NOTE
NOTE
Color-coded push buton
Locking system
1
Tip ejector button
P10, P20, P100, P200
Connecting nut
MAINTENANCE
P1000
2
4
P5000 P10mL
Piston assembly
2
3
Tip holder
Tip ejector
Figure 11
MyPIPETMAN® piston assembly (disassembled)
Servicing the Piston Assembly
You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the pipette must be adjusted and calibrated at an authorized Gilson Service Center. As the models P2 and P10 contain miniaturized parts, it is best not to disassemble these pipettes yourself.
Separate the upper part and the lower part
1. Push the ejection button 1.
2. Turn the tip ejector clockwise 2.
3. Slide and remove the tip ejector 3.
4. Unscrew the connecting nut 4.
Carefully separate the tip holder from the upper part (refer to Figure 11)
1. Remove the piston assembly, O-ring, and the seals (refer to Changing the Tip Ejector for Single Channel on page 16).
2. Leave the piston exposed, clean it with isopropanol or ethanol, and lubricate lightly.
3. Clean, autoclave, or replace the tip holder. If necessary, lightly lubricate the piston and its seals.
For P20, P100, P200, P20 models: lubricate only the useful part of the piston (20 ±5 mm length) and the O-ring (refer to Figure 10).
For P1000 model: lubricate the piston.
For P5000, P10000 models: disassemble the seals, lubricate the internal part of the seal, and then lubricate the useful part of the the piston. Do not lubricate the O-ring.
For P2 and P10 models, contain miniaturized parts. It is best not to disassemble these pipettes yourself.
4. Reassemble the pipette.
5. Tighten the connecting nut (turn counterclockwise).
6. Refit the tip ejector.
If the piston assembly is changed, the pipette must be adjusted and calibrated in at an authorized Gilson Service Center.
17
MAINTENANCE | MyPIPETMAN®
Figure 12
Lubric ate the internal part of the piston
Page 17
Chapter 8
SPARE PARTS
MyPIPETMAN SINGLE CHANNEL SPARE PARTS
MODEL
P2 -
P10 FA07012
P20 FA07013 - - FP070122 FP070121 FP070172 FP070171 FP070252 FP070251
P100 FA07014 - - FP070132 FP070131 FP070182 FP070181 FP070262 FP070261
P200 FA07015 - - FP070142 FP070141 FP070192 FP070191 FP070272 FP070271
P1000 FA07016 - - FP070152 FP070151 FP070202 FP070201 FP070282 FP070281
P5000 FA073 07 - - - - - - FP070292 FP070291
P10mL FA07308 - - - - - - FP070302 FP070301
*Translucent Adapter
SEAL+ O-RING (5 SETS)
STAINLESS STEEL EJECTOR ADAPTER
FP070212 FP070210*
FP070212 FP070210*
PLASTIC EJECTOR ADAPTER
FP070222 FP070112 FP070111 FP070162 FP070161 FP070232 FP070231
FP070222 FP070112 FP070111 FP070162 FP070161 FP070242 FP070241
WHITE TIP EJECTOR STAINLESS STEEL
DARK BLUE TIP EJECTOR STAINLESS STEEL
WHITE PLASTIC TIP EJECTOR
DARK BLUE PLASTIC TIP EJECTOR
WHITE TIP HOLDER
DARK BLUE TIP HOLDER
MyPIPETMAN MULTICHANNEL SPARE PARTS
MODEL
MyPIPETMAN P8x10 FP070311 FP070312 FG07064
MyPIPETMAN P12x10 FP070321 FP070322 FG07065
MyPIPETMAN P8x20 FP070311 FP070312 -
MyPIPETMAN P12x20 FP070321 FP070322 -
MyPIPETMAN P8x200 FP070311 FP070312 -
MyPIPETMAN P12x200 FP070321 FP070322 -
MyPIPETMAN P8x300 FP070311 FP070312 -
MyPIPETMAN P12x300 FP070321 FP070322 -
DARK BLUE PLASTIC TIP EJECTOR
WHITE PLASTIC TIP EJECTOR
EJECTOR ADAPTER
SPARE PARTS
SPARE PARTS FOR ALL MODELS
MODEL PART NUMBER
WINDOW 3DIGIT DBLUE MyPIPETMAN FP070101
WINDOW 3DIGIT WHITE MyPIPETMAN FP070102
WINDOW 3DIGIT BLUE MyPIPETMAN FP070103
WINDOW 3DIGIT RED MyPIPETMAN FP070104
WINDOW 3DIGIT DARK YELLOW MyPIPETMAN FP070105
WINDOW 3DIGIT GREEN MyPIPETMAN FP070106
FILTER FOR 5000 µL/ 10mL (x10) FE07058
LUBRICANT TUBE FOR PIPETMAN (1g) 5440011070
LUBRICANT TUBE 3.5G, CONCEPT/PM F3070902
MyPIPETMAN® | USER’S GUIDE
18
Page 18
WARRANTY
Chapter 9
WARRANTY
Gilson warrants this pipette against defects in material under normal use and service for a period of three years from the date of purchase.
This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or inadequate maintenance (contrary to the recommendations given in the user’s guide), including, but not limited to pipettes which have been subjected to physical damage, improper handling, spillage or exposure to any corrosive environment, abuse misuse or misapplication, or from ordinary wear and tear (including seal and O-rings). This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes.
THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.
19
WARRANTY | MyPIPETMAN®
Page 19
NOTES
NOTES
MyPIPETMAN® | USER’S GUIDE
20
Page 20
Specifications subject to change without notification — errors excepted.
www.gilson.com/contactus
LT801622/A | ©2019 Gilson, Inc . All rights reserved.
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