Gilson MyPipetman User Manual

USER’S GUIDE
EN
TABLE OF CONTENTS
TABLE OF CONTENTS
INTRODUCTION | 4
Parts Checklist | 4
GLP Features | 4
Description | 5
Specifications | 6
Simple Ratchet | 7
3-Position Locking System | 7
Secure Pipetting | 7
PIPETTING | 8
Fitting the Tip Ejector Adapter | 8
Fitting the Tips | 9
Pre-Rinse the Tips | 9
Aspirate | 10
Dispense | 10
Guidelines for Good Pipetting | 11
ACCESSORIES | 12
TROUBLESHOOTING | 13
Troubleshooting Table | 13
Leak Test | 14
CLEANING AND DECONTAMINATION | 14
Autoclaving | 14
Chemical Decontamination | 14
Lower Part (Volumetric Module) | 15
MAINTENANCE | 16
Changing the Tip Ejector for Single Channel | 16
Changing the Tip Holder for Single Channel | 16
Changing the Tip Ejector for Multichannel | 16
Servicing the Piston Assembly | 17
SPARE PARTS | 18
WARRANTY | 19
TABLE OF CONTENTS | MyPIPETMAN®
3
| MyPIPETMAN®
Chapter 1
INTRODUCTION
Your personally designed MyPIPETMAN® pipette has an air-displacement mechanism and is used with disposable pipette tips.
This Gilson pipette line provides:
Light and comfortable body
Unique design and configuration
Low pipetting forces ensuring ergonomics for users’ well-being
Eight single channel models cover a volume range from 0.2 μL to 10 mL.
Eight multichannel models (4 models of 8 and 12 multichannel) cover a volume range from 0.5 μL to 300 μL.
Parts Checklist
Carefully unpack your pipette and take a moment to verify that the following items are included and undamaged:
SINGLE MODELS
Your personalized MyPIPETMAN
Quick Guide
Certificate of Conformity
Safety bag
Tip ejector adapter equipped on the P2 μL and P10 μL models (fully autoclavable)
Bag of ten filters channel models
(only for P10mL
)
single
MULTICHANNEL MODELS
Your personalized MyPIPETMAN
Quick Guide
Ejector spacer for short collar tips (only for P10 μL models)
Certificate of Conformity
Safety bag
INTRODUCTION
GLP Features
The serial number is engraved on the body of the pipette. It provides unique identification of your pipette and the date of manufacture. Example: MY524CQ
The barcode on the box and the Certificate of Conformity provide traceability of your pipette.
RANDOM NUMBER
231
354
324
522
457
120
876
489
542
MONTH CODE
January A
February B
March C
April D
May E
June G
July H
August J
September K
October L
November M
December N
YEAR CODE
2019 Q
2020 R
2021 S
2022 T
2023 U
2024 W
2025 X
2026 Y
2027 Z
MY 524 C Q
MODEL
RANDOM
MONTH YEAR
NUMBER
MYPIPETMAN 524 MARCH 2019
Figure 1
Serial number
MyPIPETMAN® | USER’S GUIDE
4
Description
Please refer to the following chapters for a full description of the different parts and functions of the pipette.
INTRODUCTION
Color window
Single channel
Multichannel
Color-coded
push button
Locking system
Tip ejector
button
Personalized
handle
Color ring
Figure 2
MYPIPETMAN™ single channel and multichannel models
Connecting nut
Ejector clip
Tip holder
Ejector lock
Cover
Tip ejector
5
INTRODUCTION | MyPIPETMAN®
Specifications
NOTE
MyPIPETMAN is a high-quality pipette that offers excellent accuracy and precision especially with PIPETMAN DIAMOND Tips. The data given in the “Gilson Maximum Permissible Errors” table were obtained using PIPETMAN DIAMOND Tips. These values are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.
Each pipette is inspected and validated by qualified technicians in accordance with the Gilson quality system. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard by type testing.
The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6).
INTRODUCTION
MODEL VOLUME (µL)
MyPIPETMAN SINGLE CHANNEL
0.2
MyPIPETMAN P2
MyPIPETMAN P10
MyPIPETMAN P20
MyPIPETMAN P100
MyPIPETMAN P200
MyPIPETMAN P1000
0.5 1 2
1 5 10
2 10 20
10 50 100
20 100 200
100 500 1000
GILSON MAXIMUM PERMISSIBLE ERROR
Systematic Error (µL)
± 0.024 ± 0.025 ± 0.027 ± 0.030
± 0.025 ± 0.075 ± 0.100
± 0.10 ± 0.10 ± 0.20
± 0.35 ± 0.40 ± 0.80
± 0.50 ± 0.80 ± 1.60
± 3 ± 4 ± 8
Random Error (µL)
0.012 0.012 0.013 0.014
0.012 0.030 0.040
0.03 0.05 0.06
0.10 0.12 0.15
0.20 0.25 0.30
0.6 1.0 1.5
ISO-8655 MAXIMUM PERMISSIBLE ERROR
Systematic Error (µL)
± 0.08 ± 0.08 ± 0.08 ± 0.08
± 0.12 ± 0.12 ± 0.12
± 0.2 ± 0.2 ± 0.2
± 0.8 ± 0.8 ± 0.8
± 1.6 ± 1.6 ± 1.6
± 8.0 ± 8.0 ± 8.0
Random Error (µL)
0.04 0.04 0.04 0.04
0.08 0.08 0.08
0.1 0.1 0.1
0.3 0.3 0.3
0.6 0.6 0.6
3.0 3.0 3.0
MyPIPETMAN P5000
MyPIPETMAN P10mL
MyPIPETMAN MULTICHANNEL
MyPIPETMAN P8x10
MyPIPETMAN P12x10
MyPIPETMAN P8x20
MyPIPETMAN P12x20
MyPIPETMAN P8x200
MyPIPETMAN P12x200
MyPIPETMAN P8x300
MyPIPETMAN P12x300
500 2500 5000
1000 5000 10000
0.5 1 5 10
2 10 20
20 100 200
20 30 150 300
± 12 ± 15 ± 30
± 30 ± 40 ± 60
± 0.08 ± 0.08 ± 0.20 ± 0.20
± 0.10 ± 0.20 ± 0.40
± 0.50 ± 1.00 ± 2.00
± 1.00 ± 1.00 ± 1.50 ± 3.00
3 5 8
6 10 16
0.04 0.05 0.10 0.10
0.08 0.10 0.15
0.25 0.40 0.50
0.35 0.35 0.60 1.00
± 40 ± 40 ± 40
± 60 ± 60 ± 60
± 0.24 ± 0.24 ± 0.24 ± 0.24
± 0.4 ± 0.4 ± 0.4
± 3.2 ± 3.2 ± 3.2
± 8.0 ± 8.0 ± 8.0 ± 8.0
15.0 15.0 15.0
30.0 30.0 30.0
0.16 0.16 0.16 0.16
0.20 0.20 0.20
1.2 1.2 1.2
3.0 3.0 3.0 3.0
The data given in this table conform to the ISO 8655-2 standard. With a precise pipetting technique, the P2 model can be used to aspirate volumes as low as 0.1 μL and the P10 model as low as 0.5 μL.
MyPIPETMAN® | USER’S GUIDE
6
Chapter 2
NOTE
SETTING THE VOLUME
The volume of liquid to be aspirated is set using the volumeter. The dials are colored either black or red to indicate the position of the decimal point, depending on the model (refer to Figure 3 below).
Volume selection differs according to your configuration. To obtain maximum accuracy when setting the volume, proceed as follows:
When decreasing the volume setting, slowly reach the required setting, making sure not to overshoot the volume mark.
When increasing the volume setting, pass the required value by 1/3 of a turn and then slowly decrease to reach the volume, making sure not to overshoot the volume mark.
SETTING THE VOLUME
MODEL
MyPIPETMAN SINGLE CHANNEL
MyPIPETMAN P2 µL 0.01 µL 0.002 µL
MyPIPETMAN P10 µL 0.1 µL 0.02 µL
MyPIPETMAN P20 µL 0.1 µL 0.02 µL
MyPIPETMAN P100 µL - 0.2 µL
MyPIPETMAN P200 µL - 0.2 µL
MyPIPETMAN P1000 0.01 mL mL 0.002 mL
MyPIPETMAN P5000 0.01 mL mL 0.002 mL
MyPIPETMAN P10mL mL 0.1 mL 0.02 mL
MyPIPETMAN MULTICHANNEL
MyPIPETMAN P8x10 µL 0.1 µL 0.02 µL
MyPIPETMAN P12x10 µL 0.1 µL 0.02 µL
MyPIPETMAN P8x200 µL - 0.2 µL
MyPIPETMAN P12x200 µL - 0.2 µL
MyPIPETMAN P8x300 µL - 0.2 µL
MyPIPETMAN P12x300 µL - 0.2 µL
COLOR OF VOLUMETER NUMBERS
BLACK RED INCREMENT
P2
1
2 5
1.25 µL
P200
1 2 5
125 µL
Figure 3
Dial colors by model
Simple Ratchet
For pipettes mounted with a simple ratchet, select the volume by turning the push button (refer to Figure 4).
3-Position Locking System
For pipettes equipped with a 3-position locking system, turn the selector to your desired position (refer to Figure 5).
Single channel
model
P10
7.5 µL
P1000
P20
0 7
5
1 2
5
12.5 µL
P5000
0
7 5
0.75 mL
1.25 mL
Multichannel
model
P100
x10
0 7 5
75 µL
1
2 5
P10mL
0 7
5
7.5 mL
7.5 µL
x200
125 µL
x20
0 7
5
1 2
5
12.5 µL
1 2 5
x300
1 2 5
125 µL
Free volume selection position
1. Turn the selector to the left until the cursor reaches the unlocking icon 1.
2. Select the volume by turning the push button.
Light click volume selection position
1. Turn the selector until the cursor reaches the middle icon 2.
2. Select the volume by turning the push button.
When selecting the volume, you will hear click sounds, allowing for more precise settings. Use these sounds for final fine volume selection.
Secure Pipetting
After selecting your volume, turn the selector to the right until the cursor reaches the locking icon for secure pipetting 3.
7
SETTING THE VOLUME | MyPIPETMAN®
Figure 4
Simple ratchet
1
Figure 5
3-position locking system
2
3
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