Gilson Multi-channel microlitre pipette Pipetman® L 12-channel, 2 to 20 µl, P12x20L User guide

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VARIABLE

VOLUME

MODELS

User’s Guide

EN

TABLE OF CONTENTS

NOTICE

NOTICE

INTRODUCTION | 3

Parts Checklist | 3

TABLE OF CONTENTS

Good Laboratory Practice (GLP) Compliance | 3

Description | 4

Specifications | 5

SETTING THE VOLUME | 7

Lock System | 7

USER ADJUSTMENTS (FIXED VOLUME MODELS ONLY) | 7

PIPETTING | 8

Fitting the Tips | 8

Fitting the Tip Ejector Adapter and the Tip Ejector Extension | 9

Pre-Wet the Tips | 10

Aspirate | 10

Dispense | 10

Eject the Tips | 10

Guidelines for Good Pipetting | 10

ACCESSORIES | 11

TROUBLESHOOTING | 12

Troubleshooting Table | 12

Leak Test | 13

MAINTENANCE | 14

Maintenance for Single Channel, Variable, and Fixed Volume Models | 14

Maintenance for Multichannel Channels Models | 16

CLEANING AND DECONTAMINATION | 17

Cleaning for Single Channel and Fixed Volume Models | 17

Cleaning for Multichannel Models | 17

Autoclaving | 18

Chemical Decontamination | 18

SPARE PARTS | 19

Variable Volume, Single Channel Models | 19

Variable Volume, Multichannel Models | 20

Fixed Volume, Single Channel Models | 21

WARRANTY | 22

2

Decreased pipetting forces are due to the design of the piston assembly, which includes a
very high-quality lubricant (part number 5440011070). The use of any other lubricant voids
the warranty of this pipette.

Only current variable volume models are fully autoclavable. Autoclavable variable volume
models have a serial number that begins with NK or newer versions and a steam-sterilization
mark on the pipette.

TABLE OF CONTENTS | PIPETMAN® L

| PIPETMAN® L

121°C

MADE IN
FRANCE

Chapter 1

INTRODUCTION

PIPETMAN® L is an air displacement pipette that uses disposable tips. Designed to increase productivity
without sacrificing comfort—while still conforming to Gilson brand quality—PIPETMAN L offers a
comprehensive line with various models and features adapted to users’ needs:

● Light and comfortable body, for both right- and left-handed users

● Reduced pipetting forces* for improved ergonomics

● Full autoclavability for variable volume models

● Lockable volume for variable volume models

● 2D code for better traceability

● Name tag

● Plastic or stainless steel tip ejector options (only for single-channel, variable volume models up to
P1000L)**

*Implementation of features to improve pipetting comfort ha s no impact on th e legendary durab ility, accuracy, and precision of PIPET MAN L.

**P5000 L, P1 0mLL , and fi xed PIP ETMA N L models are only available with a plastic e jector.

Eight variable volume, single channel models cover a volume range from 0.2 µL to 10 mL.

Fifteen fixed volume, single channel models cover a volume range from 1 µL to 5000 µL.

Fourteen variable volume, multichannel models cover a volume range from 0. 5 μL to 1200 μL, including

four V-ring (VR) models, covering a volume range from 20 μL to 300 μL. PIPETMAN L V-ring (VR)
multichannel models are equipped with V-rings at the bottom of the tip holders. They create an airtight
seal between the tip and pipette making proper fit for most tip brands and low-force attachment with just
one gentle push.

Parts Checklist

After unpacking the pipette, verify that the following items were included and are undamaged:

INTRODUCTION

Single Channel Models

● PIPETMAN L

● Quick Guide

● Safety bag

● Certificate of Conformity

(including barcode sticker)

● Tip ejector adapter equipped
on P2L/P10L

● Bag of ten filters (only for
P5000L and P10mLL)

Multichannel Models

● PIPETMAN L

● Quick Guide

● Safety bag

● Certificate of Conformity

(including barcode sticker)

● Ejector spacer for D10 tips (only
for Px10L)

● V-rings (bag of four V-rings, only
for VR multichannel models)

● V-ring removal tool (only for VR
multichannel models)

Fixed Volume Models

● PIPETMAN L

● Quick Guide

● Safety bag

● Certificate of Conformity

(including bar code sticker)

● Adjustment key

Good Laboratory Practice (GLP) Compliance

The serial number is engraved on the body of the pipette. It provides unique identification of your pipette
and the date of manufacture.

Example: R H 71023

Yea r

Month Production

number

The barcode on the box and the Certificate of Conformity provides traceability
of your pipette.

In addition, a DataMatrix 2D code is engraved next to the serial number.
This code includes the ordering reference, manufacturing date, serial number,
and nominal volume.

Example: FA10039 202007 RH71023 1200

Ordering reference: 7 characters (FA10039) - Blank - Manufacturing date
(year and month): 6 characters (202007) - Blank - Serial number: 7 characters
(RH71023) - Blank - Nominal volume in µL: up to 5 characters (1200)

Using a professional 2D code reader, this information can be integrated into any
traceability or asset management system.

Figure 1

Serial number location

PIPETMAN® L | USER’S GUIDE

3

Description

Please refer to following chapters for a complete description of the different parts and functions of the

pipette.

Variable Volume Models

Fixed Volume Models

INTRODUCTION

Single Channel

1

1

Multichannel

1

1

12

2

3

4

5

7

5

7

2

3

4

5

2

3

4

6

9

3

4

5

7

8

8

8

10

7

8

11

11

11

13

1

Color-coded push button

2

Thumbwheel

3

Tip ejector button

4

Handle

Connecting nut

5

6

Ejector clip

7

Tip holder(s)

8

Tip ejector

Ejector support

9

Cover

10

PIPETMAN® DIAMOND Tip(s)

11

12

Graduated cover

V-rings (for VR multichannel models only)

13

Figure 2

PIPETMAN® L single channel, multichannel, VR multichannel, and fixed volume model components

Name Tag

You can identify your pipette with a name tag:

1. Remove the name tag and write on it in order to
identify the pipette.

2. Position the name tag in the slot.

3. Clip the window back into place.

11

INTRODUCTION | PIPETMAN® L

4

Figure 3

Name tag placement for pipette identification single
channel, multichannel, and fixed volume models

Specifications

PIPETMAN L is a high-quality pipette that offers excellent accuracy and precision. The figures given in the
“Gilson Maximum Permissible Errors” table were obtained using PIPETMAN® DIAMOND Tips.

These values are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.

Each pipette is inspected and validated by qualified technicians in accordance with the Gilson Quality
System. Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655
standard, by type testing.

The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6).

Maximum Permissible Errors

PIPETMAN® L SINGLE CHANNEL—VARIABLE VOLUME MODELS

Model

P2L

P10L

P20L D200 FA10003M FA10003P2 10

P100L D200 FA10004M FA10004P

PIPETMAN®
DIAMOND
Tips

D10
DL10

D10
DL10

With
Stainless
Steel
Ejector

FA10001M FA10001P

FA10002M FA10002P

With
Plastic
Ejector

Part Number

Vol.
(µL)

0.2

0.5
1
2

0.5
1
5
10

20

10
50
100

Systemat ic
Error
(µL)

± 0.024
± 0.025
± 0.027
± 0.030

± 0.040
± 0.025
± 0.075
± 0.100

± 0.10
± 0.10
± 0.20

± 0.35
± 0.40
± 0.80

Random
Error
(µL)

≤ 0.012
≤ 0.012
≤ 0.013
≤ 0.014

≤ 0.016
≤ 0.012
≤ 0.030
≤ 0.040

≤ 0.030
≤ 0.050
≤ 0.060

≤ 0.10
≤ 0.12
≤ 0.15

Maximum Permissible Errors

Gilson ISO 8655

Systemat ic
Error
(%)

± 12.0
± 5.0
± 2.7
± 1.5

± 8.0
± 2.5
± 1.5
± 1.0

± 5.0
± 1.0
± 1.0

± 3.5
± 0.8
± 0.8

Random
Error
(%CV*)

≤ 6.0
≤ 2.4
≤ 1.3
≤ 0.7

≤ 3.2
≤ 1.2
≤ 0.6
≤ 0.4

≤ 1.5
≤ 0.5
≤ 0.3

≤ 1.0
≤ 0.24
≤ 0.15

Systema tic
Error
(µL)

± 0.08
± 0.08
± 0.08
± 0.08

± 0.12
± 0.12
± 0.12
± 0.12

± 0.2
± 0.2
± 0.2

± 0.8
± 0.8
± 0.8

Random
Error
(µL)

≤ 0.04
≤ 0.04
≤ 0.04
≤ 0.04

≤ 0.08
≤ 0.08
≤ 0.08
≤ 0.08

≤ 0.1
≤ 0.1
≤ 0.1

≤ 0.3
≤ 0.3
≤ 0.3

Systemat ic
Error
(%)

± 40.0
± 16.0
± 8.0
± 4.0

± 24.0
± 12.0
± 2.4
± 1.2

± 10.0
± 2.0
± 1.0

± 8.0
± 1.6
± 0.8

Random
Error
(%CV*)

≤ 20.0
≤ 8.0
≤ 4.0
≤ 2.0

≤ 16.0
≤ 8.0
≤ 1.6
≤ 0.8

≤ 5.0
≤ 1.0
≤ 0.5

≤ 3.0
≤ 0.6
≤ 0.3

INTRODUCTION

P200L D200 FA10005M FA10005P

P1000L D1000 FA10006M FA10006P

P5000L D5000 FA10007

P10mLL D10mL FA10008

PIPETMAN® L MULTICHANNEL MODELS

Model

P8x10 L

P12x1 0L FA10 014

P8x20L

P12x20L FA10010

P8x200L

P12x200L FA10 012

P8 x20 0LVR

P12x200LVR FA10 036

P8x300L

P12x300L FA10 016

P8x300LVR

P12x300LVR FA10 038

P12x1200L

P12x1200L FA10040

PIPETMAN®

DIAMOND Tips

D10
DL10

DL10
D200

D200

D200

D300

D300

D1200

Part

Number

FA10 013

FA10009

FA10 011

FA10 035

FA10 015

FA10 037

FA10 039

20
100
200

100
500
1000

500
2500
5000

1000
5000
10000

Vol.

(µL)

0.5
1
5
10

2
10
20

20
100
200

20
100
200

20
30
150
300

20
30
150
300

100
120
600
1200

± 0.50
± 0.80
± 1.60

± 3.0
± 4.0
± 8.0

± 12
± 15
± 30

± 30
± 40
± 60

Systemat ic
error
(µL)

± 0.08
± 0.08
± 0.20
± 0.20

± 0.10
± 0.20
± 0.40

± 0.50
± 1.00
± 2.00

± 0.50
± 1.00
± 2.00

± 1.00
± 1.00
± 1.50
± 3.00

± 1.00
± 1.00
± 1.50
± 3.00

± 6.0
± 6.0
± 8.0
± 10.0

≤ 0.20

± 2.5

≤ 0.25

± 0.8

≤ 0.30

± 0.8

≤ 0.6

± 3.0

≤ 1.0

± 0.8

≤ 1.5

± 0.8

≤ 3

± 2.4

≤ 5

± 0.6

≤ 8

± 0.6

≤ 6

± 3.0

≤ 10

± 0.8

≤ 16

± 0.6

Gilson ISO 8655

Random
error
(µL)

≤ 0.04
≤ 0.05
≤ 0.10
≤ 0.10

≤ 0.08
≤ 0.10
≤ 0.15

≤ 0.25
≤ 0.40
≤ 0.50

≤ 0.25
≤ 0.40
≤ 0.50

≤ 0.35
≤ 0.35
≤ 0.60
≤ 1.00

≤ 0.35
≤ 0.35
≤ 0.60
≤ 1.00

≤ 0.9
≤ 0.9
≤ 1.2
≤ 1.5

≤ 1.0
≤ 0.25
≤ 0.15

≤ 0.6
≤ 0.2
≤ 0.15

≤ 0.6
≤ 0.2
≤ 0.16

≤ 0.6
≤ 0.2
≤ 0.16

Maximum Permissible Errors

Systemat ic
error
(%)

± 16.0
± 8.0
± 4.0
± 2.0

± 5.0
± 2.0
± 2.0

± 2.5
± 1.0
± 1.0

± 2.5
± 1.0
± 1.0

± 5.0
± 3.3
± 1.0
± 1.0

± 5.0
± 3.3
± 1.0
± 1.0

± 6.0
± 5.0
± 1.33
± 0.83

Random
error
(%CV*)

≤ 8.0
≤ 5.0
≤ 2.0
≤ 1.0

≤ 4.0
≤ 1.0
≤ 0.75

≤ 1.25
≤ 0.4
≤ 0.25

≤ 1.25
≤ 0.4
≤ 0.25

≤ 1.75
≤ 1.17
≤ 0.4
≤ 0.33

≤ 1.75
≤ 1.17
≤ 0.4
≤ 0.33

≤ 0.9
≤ 0.75
≤ 0.2
≤ 0.13

± 1.6
± 1.6
± 1.6

± 8.0
± 8.0
± 8.0

± 40
± 40
± 40

± 60
± 60
± 60

Systemat ic
error
(µL)

± 0.24
± 0.24
± 0.24
± 0.24

± 0.4
± 0.4
± 0.4

± 3.2
± 3.2
± 3.2

± 3.2
± 3.2
± 3.2

± 8.0
± 8.0
± 8.0
± 8.0

± 8.0
± 8.0
± 8.0
± 8.0

± 32
± 32
± 32
± 32

≤ 0.6
≤ 0.6
≤ 0.6

≤ 3.0
≤ 3.0
≤ 3.0

≤ 15.0
≤ 15.0
≤ 15.0

≤ 30.0
≤ 30.0
≤ 30.0

Random
error
(µL)

≤ 0.16
≤ 0.16
≤ 0.16
≤ 0.16

≤ 0.2
≤ 0.2
≤ 0.2

≤ 1.2
≤ 1.2
≤ 1.2

≤ 1.2
≤ 1.2
≤ 1.2

≤ 3.0
≤ 3.0
≤ 3.0
≤ 3.0

≤ 3.0
≤ 3.0
≤ 3.0
≤ 3.0

≤ 12.0
≤ 12.0
≤ 12.0
≤ 12.0

± 8.0
± 1.6
± 0.8

± 8.0
± 1.6
± 0.8

± 8.0
± 1.6
± 0.8

± 6.0
± 1.2
± 0.6

Systemat ic
error
(%)

± 48.0
± 24.0
± 4.8
± 2.4

± 20.0
± 4.0
± 2.0

± 16.0
± 3.2
± 1.6

± 16.0
± 3.2
± 1.6

± 40.0
± 26.67
± 5.33
± 2.67

± 40.0
± 26.67
± 5.33
± 2.67

± 32.00
± 26.67
± 5.33
± 2.67

≤ 3.0
≤ 0.6
≤ 0.3

≤ 3.0
≤ 0.6
≤ 0.3

≤ 3.0
≤ 0.6
≤ 0.3

≤ 3.0
≤ 0.6
≤ 0.3

Random
error
(%CV*)

≤ 32.0
≤ 16.0
≤ 3.2
≤ 1.6

≤ 10.0
≤ 2.0
≤ 1.0

≤ 6.0
≤ 1.2
≤ 0.6

≤ 6.0
≤ 1.2
≤ 0.6

≤ 15.0
≤ 10.0
≤ 2.0
≤ 1.0

≤ 15.0
≤ 10.0
≤ 2.0
≤ 1.0

≤ 12.0
≤ 10.0
≤ 2.0
≤ 1.0

*CV means Coecient of Variation

PIPETMAN® L | USER’S GUIDE

5

NOTICE

NOTICE

PIPETMAN® L FIXED VOLUME MODELS

INTRODUCTION

Model

F1L

F2L

F5L

F10L

F20L D200 FA10021 20 ± 0.10 ≤ 0.05 ± 0.5 ≤ 0.25 ± 0.20 ≤ 0.10 ± 1.0 ≤ 0.5

F25L D200 FA10022 25 ± 0.20 ≤ 0.07 ± 0.8 ≤ 0.28 ± 0.50 ≤ 0.20 ± 2.0 ≤ 0.8

F50L D200 FA10023 50 ± 0.35 ≤ 0.12 ± 0.7 ≤ 0.24 ± 0.50 ≤ 0.20 ± 1.0 ≤ 0.4

F100L D200 FA10024 100 ± 0.55 ≤ 0 .15 ± 0.55 ≤ 0.15 ± 0.80 ≤ 0.30 ± 0.8 ≤ 0.3

F200L D200 FA10025 200 ± 1.2 ≤ 0.30 ± 0.6 ≤ 0.15 ± 1.60 ≤ 0.60 ± 0.8 ≤ 0.3

F250L D300 FA10026 250 ± 1.50 ≤ 0.75 ± 0.6 ≤ 0.3 ± 4.00 ≤ 1.50 ± 1.6 ≤ 0.6

F300L D1000 FA10027 300 ± 2.4 ≤ 0.50 ± 0.8 ≤ 0.17 ± 4.00 ≤ 1.50 ± 1.33 ≤ 0.5

F400L D1000 FA10028 400 ± 2.4 ≤ 0.80 ± 0.6 ≤ 0.2 ± 4.00 ≤ 1.50 ± 1.0 ≤ 0.4

F500L D1000 FA10029 500 ± 3.0 ≤ 0.80 ± 0.6 ≤ 0.16 ± 4.00 ≤ 1.50 ± 0.8 ≤ 0.3

F1000L D1000 FA10030 1000 ± 5.0 ≤ 1.3 ± 0.5 ≤ 0.13 ± 8.00 ≤ 3.00 ± 0.8 ≤ 0.3

F5000L D5000 FA10031 5000 ± 20.0 ≤ 7. 0 ± 0.4 ≤ 0.14 ± 40.00 ≤ 15.00 ± 0.8 ≤ 0.3

PIPETMAN®
DIAMOND Tips

D10
DL10

D10
DL10

D10
DL10

D10
DL10

Part
Number

FA10017 1 ± 0.02 ≤ 0.015 ± 2.0 ≤ 1.50 ± 0.05 ≤ 0.05 ± 5.0 ≤ 5.0

FA10018 2 ± 0.05 ≤ 0.02 ± 2.5 ≤ 1.0 ± 0.08 ≤ 0.04 ± 4.0 ≤ 2.0

FA10019 5 ± 0.050 ≤ 0.025 ± 1.0 ≤ 0.5 ± 0.125 ≤ 0.075 ± 2.5 ≤ 1.5

FA10020 10 ± 0.06 ≤ 0.03 ± 0.6 ≤ 0.3 ± 0.12 ≤ 0.08 ± 1.2 ≤ 0.8

Vol.
(µL)

Systema tic
Error
(µL)

Gilson ISO 8655

Random
Error
(µL)

Systema tic
Error
(%)

Random
Error
(%CV*)

Systema tic
Error
(µL)

Random
Error
(µL)

Systema tic
Error
(%)

Random
Error
(%CV*)

*CV means Coecient of Variation

The data given in the tables conform to the ISO 8655-2 standard.

With a precise pipetting technique (refer to Guidelines for Good Pipetting, page 10), the P2L may be
used to aspirate volumes as low as 0.1 µL.

For variable volume, single channel models from P2L to P1000L, each model has two dierent part
numbers to identify the kind of tip ejector installed.

Part numbers ending with M correspond to a pipette with a stainless steel ejector, and part numbers
ending with P correspond to pipettes with plastic ejector.

Example: For a P10L with the plastic tip ejector the part number is FA10002P. For the same pipette with
a stainless steel tip ejector, the part number is FA10002M.

P5000L and P10mLL are equipped with plastic tip ejectors.

6

INTRODUCTION | PIPETMAN® L

NOTICE

P2L

P10L

P20L

P100L

Chapter 2

NOTICE

0
7

5

Px10L

7.5 µL

1
2

5

Px20L

12.5 µL

Px200LVR

SETTING THE VOLUME

The volume of liquid to be aspirated is set using the volume display. The dial colors are either black or red
to indicate the position of the decimal point, depending on the model (refer to Figure 4).

SETTING THE VOLUME

Model

P2L µL 0.01 µL 0.002 µL

P10L to P20L – P8X10L ­P12X10L

P100L – P200L
P8X200L - P12X200L
P8X300L - P12X300L

P8X200LVR - P12X200LVR
P8X300LVR - P12X300LVR

P1000L 0.01 mL mL 0.002 mL

P8x1200L - P12x1200L 0.01 mL mL 0.002 mL

P5000L 0.01 mL mL 0.002 mL

P10mLL mL 0.1 mL 0.02 mL

Color of VolumeterN umbers

BLAC K RED INCREMENT

µL 0.1 µL 0.02 µL

µL - 0.2 µL

µL - 0.2 µL

Lock System

For additional safety, the volume selected is lockable.

1. Unlock the thumbwheel by pushing it up with your
thumb.

2. Set the volume by turning the thumbwheel.
The thumbwheel can be turned using one hand to
slowly reach the required setting.

3. Lock the newly set volume by pushing down on the
thumbwheel.

To obtain maximum accuracy when setting the volume:

● When decreasing the volume setting, slowly reach
the required setting, making sure not to overshoot
the mark.

● When increasing the volume setting, pass the required
value by 1/3 of a turn and then slowly decrease to reach
the volume, making sure not to overshoot the mark.

Single channel

models

1

0

1

2
5

1.25 µL

P200L

1
2
5

125 µL

7

5

7.5 µL

P1000L

0

7
5

0.75 mL

12.5 µL

P5000L

1.25 mL

0

2

7

5

5

75 µL

P10mLL

1

2
5

7.5 mL

Figure 4

Dial colors by model

1

2

Figure 5

Volume unlock and unlock

Protection of your pipette and
your work:

If step 3 is forgotten, the volume
selected will be automatically
locked during the next purge.

Multichannel

models

Px10L

Px20L

0
7

5

Px200L

1
2
5

125 µL

7.5 µL

0
7

5

Px300L

Px300LVR

125 µL

1
2

5

12.5 µL

1
2
5

Px1200L

0

7
5

0.75 µL

3

USER ADJUSTMENTS

(FIXED VOLUME MODELS ONLY)

PIPETMAN L fixed volume models are factory calibrated
using distilled water and very high precision balances.
The nominal volume may be adjusted slightly to
compensate for liquids of different density or viscosity.

To accommodate for density or viscosity, an adjustment
of one full turn of the adjustment key in either direction
equals:

± 0.05 μL (F1L to F2L)
± 0.2 μL (F5L to F10L)
± 0.5 μL (F20L to F25L)
± 2.0 μL (F50L to F100L)
± 5.1 μL (F200L to F250L)
± 20 μL (F300L to F1000L)
± 102 μL (F5000L)

The cover is graduated in tenths of the key revolution
(letters A, B,..., J) and is read from left to right. Each
small graduation mark represents 0.25 of the distance
between each letter.

Chapter 3

Adjustment of the factory
calibration must only be
performed using the supplied
adjustment key.

Figure 6

PIPETMAN ® L fixed volume model adjustment—hold
the key in the highest position to avoid banging it
into the hook

PIPETMAN® L | USER’S GUIDE

7

NOTICE

To adjust the nominal volume setting to compensate for a
specific density or viscosity, engage the two hooks of the
spanner tool inside the two small holes on the top of the
body, and then turn the key slowly:

Clockwise

PIPETTING

● Clockwise to decrease the volume; making sure not

to overshoot the mark.

● Counterclockwise to increase the volume; pass
the required value by 1/4 turn, and then slowly
decrease the volume to reach the required setting.

Example: When using an F100L to aspirate a particularly
viscous solution, you may determine gravimetrically that
the volume delivered is 98.8 μL, and the display reads
“H”. Therefore, you wish to increase the volume dispensed
by 1.2 μL. As the interval between each letter on the
calibration display represents 0.2 μL for the F100L (1/10 of
2 μL), the calibration tool must be turned three units until
the display reads “B”.

It is possible to alter the volume by more than one full turn
in the counterclockwise direction. In this case, remember
to readjust the same number of turns when it is necessary
to return to the original setting.

Check the new volume gravimetrically. If the volumes
delivered are still not acceptably close to the desired
value, make another slight adjustment in the relevant
direction. Be sure to change tips between volume setting

adjustments and to pre-wet each new tip.

After adjusting the nominal volume to compensate for a
particular solution, the nature of the liquid can be noted
on the name tag. For further information about how to
identify your pipette, please refer to INTRODUCTION, part
Personal Label, on page 4.

Chapter 4

Graduations

Counterclockwise

Figure 7

Adjust PIPETMAN® L fixed volume setting to
compensate for a specific density or viscosity

Figure 8

Identify the liquid used with the name tag

PIPETTING

It is recommended to use PIPETMAN® DIAMOND Tips for optimum performance. These tips are
made from pure polypropylene. Plastic tips are for a single application—they must not be cleaned
for reuse.

Fitting the Tips

Single Channel Models

To fit a new PIPETMAN DIAMOND tip, push the tip holder into the tip using a slight twisting motion
to ensure a firm and airtight seal.

PIPETMAN DIAMOND Tip

Compatibility for single channel

variable volume models

P2L, P10L D10, DL10, DF10ST, DFL10ST

P20L D200, DF30ST

P100L D200, DF100ST

P200L D200, D300, DF200ST, DF300ST

P1000L D1000, D1200, DF1000ST, DF1200ST

P5000L D5000

P10mLL D10mL

F1L, F2L, F5L, F10L D10, DL10, DF10ST, DFL10ST

F20L, F25L D200, DF30ST

F50L, F100L D200, DF100ST

F200L D200, DF200ST

F250L D300, DF300ST

F300L, F400L, F500L, F1000L D1000, DF1000ST

F5000 D5000

Figure 9

PIPETMAN® DIAMOND Tip compatibility charts for single channel models

D5000 and D10mL PIPETMAN Diamond tips do not have any filter. P5000L and P10mLL models are sold
with a bag of 10 filters. Bags of filters are also available as accessories. Refer to Accessories on page 11.

The filters, made of polyethylene, oer the same properties as the filters which are inside the Diamond
filter tips. They maintain the pipette’s original accuracy and precision by creating an ecient barrier to
aerosols or corrosive vapours such as acids which could damage mechanical parts.

Insert the filter directly in the pipette tip holder.

Change the filter every day or more frequently depending on the intensity of use and/or concentration
of the solution.

Filters are not autoclavable and must be disposed of after use.

PIPETMAN DIAMOND Tip

Compatibility for fixed volume models

8

PIPETTING | PIPETMAN® L

Multichannel Models

NOTE

NOTICE

PIPETMAN DIAMOND Tips: TIPACK AND TOWERPACK

PIPETMAN DIAMOND tips are best fitted with the ROCKY RACK™ technique, invented by Gilson, available
only in our TIPACK and TOWER PAC K .

PIPETMAN DIAMOND Tip

Compatibility for multichannel models

P8x10L, P12x10L D10*, DL10, DF10ST, DFL10ST

P8x20L, P12x20L DL10, D200, DFL10ST, DF30ST

P8x200L, P12x200L D200, D300, DF200ST, DF300ST

P8x200LVR, P12x200LVR D200, D300, DF200ST, DF300ST

P8x300L, P12x300L D200, D300, DF200ST, DF300ST

P8x300LVR, P12x300LVR D200, D300, DF200ST, DF300ST

P8x1200L, P12x1200L D1200, DF1200ST

* Using a new ejector spacer, you can fit a D10 tip (see below)

Figure 10

PIPETMAN ® DIAMOND Tip compatibility charts for
multichannel models

Figure 11

ROCKY RACK™ technique

No need to fit the
tips with ROCKY
RACK technique
for the PIPETMAN L
multichannel V-ring
models.

V-Ring Multichannel Models

V-ring multichannel models complete the PIPETMAN L range of multichannel pipettes with four models of
8 and 12 channels covering a volume range from 20 μL to 300 μL.

PIPETMAN L multichannel V-ring models have a leak-free tip holder design. Each VR multichannel model
has V-rings at the bottom of the tip holders that create an airtight seal between the tip and pipette to get
a proper fit for most tip brands and low force attachment with just one gentle push.

PIPETTING

Fitting the Tip Ejector Adapter and the Tip Ejector Extension

Single Channel Models

For P2L and P10L pipettes equipped with a stainless steel tip ejector, a dual-position adapter is required
to fit and eject DL10 tips (long collar tips) and D10 tips (short collar tips).

A

To fit the adapter for short and long collar tips (refer to Figure 12):

1. Pull the adapter down from the stainless steel tip ejector.

2. Turn the adapter 180°.

3. Refit the adapter so that the end of the stainless steel tip ejector
engages the shorter or longer slot of the adapter.

For the F1L, F2L, F5L, F10L, P2L, and P10L equipped with a plastic
tip ejector, a tip ejector extension is supplied to fit with D10 tips (short
collar tips). Refer to Figure 12.

B

To fit a tip ejector extension:

1. Slide the extension over the tip holder.

2. Push the extension firmly onto the end of the tip ejector
until it clicks into place.

To remove a tip ejector extension:

C

1. Gently twist the adapter.

2. Pull it away from the pipette.

Multichannel Models

Fitting the Ejector Adapter for P8x10L and
P12x10L Multichannel Models

Depending on the tip used, either D10
or DL10, you may have to fit the ejector
adapter. Long collar tips can be inserted
without it.

1. Remove the tip ejector: keep both
ejector locks depressed 1, and then
pull the tip ejector down 2.

2. Fit the ejector adapter 3 and click it to
the tip ejector.

3. Gently re-insert the tip ejector
vertically into the rails of the ejector
support 4.

Figure 13

Fitting ejector adapter on multichannel models

1

2

180°

Short collar

tips

A

Long collar

tips

B

To fit

To remove

Figure 12

Tip ejector dual position adapter
and extension for P2L and P10L

3

4

C

Long collar tips (DL10) can
be used without a tip ejector
adapter.

PIPETMAN® L | USER’S GUIDE

9

PIPETTING

NOTE

NOTE

Pre-Wet the Tips

Pre-wetting the tips before pipetting helps prepare the tips for the best pipetting performance. Ideally, the
pre-wet includes both immersing the tip in the liquid and performing one pipetting step.

Pre-wetting the tips helps ensure that volumes that you pipette will achieve accuracy and precision within
specifications.

Aspirate

1. Press the push button to the first stop (this
corresponds to the set volume of liquid).

2. Hold the pipette vertically and immerse
the tip in the liquid (see immersion depth
table below).

3. Release the push button slowly and
smoothly to top position to aspirate the set
volume of liquid.

4. Wait one second (time depends on model,
see table below),and then withdraw the
pipette tip from the liquid. You may wipe
any droplets away from the outside of the
tip using a medical wipe; however, if you
do so, take care to avoid touching the tip’s
orifice.

For the multichannel models,
use a reagent reservoir (refer to

ACCESSORIES on page 11).

Prepare Aspirate Purge H ome

Rest position

First stop

Second stop

Figure 14

Pipetting motion - Aspirate and Dispense

Dispense

Dispense

1. Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).

2. Press the push button slowly and smoothly to the first stop.

3. Wait for at least a second, then press the push button to the second stop to expel any residual liquid
from the tip. Keep the push button pressed fully and while removing the pipette, draw the tip along
the inside surface of the vessel.

4. Release the push button smoothly. Eject the tip by pressing firmly on the tip ejector button.

For the multichannel models, use a reagent reservoir (refer to ACCESSORIES on page 11).

Eject the Tips

Before you start to pipette, you can adjust the tip ejector button according to your preferences.

1. Position the tip ejector button by
rotating the tip ejector button to the
most comfortable position: left, right,
or middle.

2. Activate the tip ejector. You can either
push the tip ejector button with the tip
of the thumb as usual, or with the base

Left

Middle Right

of your thumb for more comfort.

Figure 15

Adjustable tip ejector button positions

Guidelines for Good Pipetting

● Make sure that you operate the push button slowly and smoothly.

● When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table below).

10

Model

P2L, P10L, P8x10L, P12x10L, F1L, F2L, F5L, F10L 1 1

P20L, P8x20L, P12x20L, P8x200L, P12x200L, P8x200LVR, P12x200LVR, F20L,
F25L

P100L, P200L, P8x300L, P12x300L, P8x300LVR, P12x300LVR, F50L, F100L,
F200L, F250L

P1000L, P8x1200L, P12x1200L, F300L, F400L, F500L, F1000L 2–4 2–3

P5000L 3–6 4–5

P10mLL 5–7 4–5

F5000L 3-6 1

Immersion Depth
(Millimeters)

2–3 1

2–4 1

PIPETTING | PIPETMAN® L

Wait Ti me
(Seconds)

NOTICE

NOTICE

● Change the tip before aspirating a different liquid, sample, or reagent

● Change the tip if a droplet remains at the end of the tip from the previous pipetting operation

● Pre-wet each new tip with the liquid to be pipetted

● Liquid should never enter the tip holder; to prevent this:
○ Press and release the push button slowly and smoothly
○ Never turn the pipette upside down
○ Never lay the pipette on its side when there is liquid in the tip

● If you use the same tip with a higher volume, pre-wet the tip

● For volatile solvents, saturate the air cushion in your pipette by aspirating and dispensing the solvent

repeatedly before aspirating the sample

● When the temperature of the liquid is different from the ambient temperature, pre-wet the tip several

times before use

● You may remove the tip ejector (refer to Changing the Tip Ejector on page 14) to aspirate from very

narrow tubes

● After pipetting acids or other corrosive liquids that emit vapors, remove the tip ejector and tip holder,

rinse with distilled water, dry, and lubricate the piston (refer to MAINTENANCE on page 14). For the
P1000L model, use a specific tip holder equipped with a filter to increase the lifetime of the piston
(refer to ACCESSORIES on page 11)

● Do not pipette liquids with temperatures above 70°C or below 4°C. The pipette can be used between

4°C and 40°C but the specifications may vary according to the temperature (refer to the ISO 8655-2
standard for conditions of use)

PIPETMAN L should be held in the vertical position.

For multichannel P8x1200L and P12 x1200 L, reverse pipetting technique is not recommended at the
nominal volume of the pipette when used with filter tips.

For detailed information about pipetting techniques, refer to the Gilson Guide to Pipetting, which is
available on the Gilson website (www.gilson.com).

ACCESSORIES

Chapter 5

ACCESSORIES

To make pipetting more comfortable and more convenient, Gilson has developed several accessories:

Description Part Number

Plastic ejector P2/P10 with adapter F107027

Plastic ejector P20 F107028

Plastic ejector P100 F107029

Plastic ejector P200 F107030

Plastic ejector P1000 F107031

Pipette stands allow users to store pipettes
vertically to avoid the possibility of liquid running
back into the pipette.

Description Part Number

CARROUSEL pipette Stand (7 pipettes) F161401

TRIO Pipette Stand (3 pipettes) F161405

Universal Multichannel Stand* F161417

SINGLE Pipette Holder F161406

* Universal multichannel stand is for multichannel models only.

To identify or personalize your pipette, COLORIS™
Identification Clips are available.

Description Part Number

COLORIS Identification Clips (mixed colors set
of 10)

COLORIS Identification Clips (red, set of 10) F161302

COLORIS Identification Clips (yellow, set of 10) F161303

COLORIS Identification Clips (green, set of 10) F161304

COLORIS Identification Clips (blue, set of 10) F161305

COLORIS Identification Clips (white, set of 10) F161306

F161301

With the JIMMY ™ Microtube Opener, a hands-free
microtube opener, you can open both snap-cap
and screw-cap microtubes.

Description Part Number

JIMMY Microtube Opener (set of 3) F144983

Disposable Reagent Reservoirs are ideal for
dispensing reagent with multichannel pipettes.

Description Part Number

Reagent reservoir 25 mL, x100 F267660

Reagent reservoir 50 mL, x100 F267670

For P500L and P10mLL models, disposable
filters maintain the pipette’s original accuracy
and precision by creating an efficient barrier
to aerosols or corrosive vapours such as acids
which could damage mechanical parts.

Description Part Number

Bag of 10 Filters for P5000L/P10mLL F161280

Bag of 100 Filters for P5000L/P10mLL F161241

To protect the piston when pipetting corrosive
liquids, you can use a specific tip holder and
filter for the model P1000L.

Description Part Number

Corrosion Protection Kit
(tip holder + a bag of 10 filters)

Corrosion Protection Kit Filter (Bag of 10)* F144571

Corrosion Protection Kit Filter (Bag of 50)* F144572

*Filter s can be inserte d in the specific tip holder provided in t he Corros ion
Protect ion Kit (pa rt Number F144570)

CARROUSEL™

SINGLE™

Universal

Multichannel

Stand

Identification Clips

TRIO™

PIPETMAN® L | USER’S GUIDE

F144570

Corrosion

protection

COLORIS™

kit

JIMMY™

Microtube

Opener

11

TROUBLESHOOTING

NOTE

WARNING

NOTE

NOTE

Chapter 6

TROUBLESHOOTING

A quick inspection of the pipette may help you to detect a potential problem.

You may download the Two Minute Inspection Poster LT800520 from the Gilson website
(www.gilson.com), which shows how to perform a quick diagnosis of your pipette.

Before returning any pipette to your local Gilson Service Center, ensure that it is completely
free of chemical, biological, or radioactive contamination. Refer to CLEANING AND

DECONTAMINATION on page 17. Please use the included safety bag to return the pipette to your

local Gilson-authorized service center (Click here to find your Gilson Service Center).

Troubleshooting Table

The following table may help you to identify and correct a potential problem you might encounter.

Please make sure the tips are fitted properly. Refer to Fitting the Tips on page 8.

For Single Channel Variable, Fixed Volume, and Multichannel Models

Symptom Possible Cause Action Model*

Pipette is leaking
sample

Pipette will not
aspirate

Pipetted volume is
inaccurate

Tips fall o or do not
fit correctly

Pipetting seize up Piston needs lubricant Lubricate piston assembly S

Damaged tip holder Replace the tip holder S

Worn O-ring or seal Replace both parts and lubricate S

Damaged or upside-down V-ring Replace the V-ring(s) and perform a leak test M VR

Worn O-ring Replace both parts and lubricate S

Damaged tip holder Replace the tip holder S

Connecting nut is loose Tighten connecting nut S+M

Damaged or corroded piston Return pipette to supplier S

Improper repair or assembly Refer to page 14 - MAINTENANCE S

Improper repair or assembly Refer to page 14 - MAINTENANCE S

Connecting nut is loose Tighten connecting nut S+M

Tip holder is loose Tighten connecting nut S

Incorrect operator technique Operator training S+M

Damaged or corroded piston(s) Return pipette to supplier S

Damaged tip holder(s) Replace the tip holder(s) S

Worn O-ring or seal Replace both parts and lubricate S

Low quality tips Use PIPETMAN DIAMOND Tips S+M

Damaged tip holder(s) Replace the tip holder(s) S+M

Damaged tip ejector Replace the tip ejector S+M

Damaged or upside-down V-Ring Replace the V-ring(s) with the removal tool M VR

Dirty tip holder Clean the tip holder with isopropanol or ethanol S+M

Tip ejector is loose Assemble the tip ejector properly S

Ejector lock is misaligned Align the ejector lock S

12

*S: Sin gle cha nnel pipette s / M: Multichan nel pipettes / M V R: Multichan nel with V-rings p ipettes

If you cannot solve the problem, contact your local Gilson-authorized service center.

TROUBLESHOOTING | PIPETMAN® L

Leak Test

This test may be performed at any time to check that the pipette does not leak, especially after performing
a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal, and
the V-ring for VR multichannel models.

For the P2L to P200L single channel models, and the F1L to F250L fixed volume models:

1. Fit a PIPETMAN DIAMOND Tip.

2. For the variable volume models, set the pipette to the maximum volume given in the specifications,
and pre-rinse.

3. Aspirate the set volume from a beaker of distilled water.

4. Maintain the pipette in the vertical position and wait for 20 seconds :

● If a water droplet appears at the end of the tip, there is a leak.

● If you see no droplets, re-immerse the tip below the surface of water. The water level inside the
tip should remain constant; if the level goes down there is a leak.

For the P1000L, P5000L, and P10mLL single channel models, and the F300L to F5000L fixed volume models:

1. Fit a PIPETMAN DIAMOND tip.

2. For the variable volume models, set the pipette to the maximum volume given in the specifications.

3. Aspirate the set volume from a beaker of distilled water.

4. Maintain the pipette in the vertical position and wait for 20 seconds:

● If a water droplet appears at the end of the tip, there is a leak.

For the multichannel models:

1. Fit the PIPETMAN DIAMOND tip.

2. Set the pipette to the maximum volume given in the
specifications, and pre-wet the tips.

3. Aspirate the set volume from a reagent reservoir of distilled
water.

4. Maintain the pipette in the vertical position and wait for
20 seconds:

● If a water droplet appears at the end of the tip, there is
a leak.

● If you see no droplets, for volumes below 200 µL,
re-immerse the tip below the surface of water.

● The water level inside the tip should remain constant; if the
level goes down there is a leak.

Figure 16

PIPETMAN® L multichannel leak test

no leak

TROUBLESHOOTING

PIPETMAN® L | USER’S GUIDE

13

Chapter 7

NOTICE

NOTICE

MAINTENANCE

Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of
performance.

Gilson recommends maintenance and calibration at least annually, more frequently as needed,
depending on use. The instructions provided below detail how to perform some basic maintenance
yourself. Please contact your local Gilson service provider (Click here to find your local Gilson

representative) to have Gilson provide maintenance and calibration for you or to ask any service

related questions.

Maintenance is limited to:

● Cleaning or decontamination (refer to CLEANING AND DECONTAMINATION on page 17)

● Replacing spare parts

● Greasing the piston assembly

MAINTENANCE

Maintenance for Single Channel, Variable, and Fixed Volume Models

PIPETMAN P2L and P10L, F1L , F2L , F5L and F10L should not be disassembled, so you may only replace
the push button, tip ejector, dual position tip ejector and its adapter. With these pipettes if the tip holder is
damaged, the piston may also be damaged.

Changing the Tip Ejector

To remove:

1. The tip ejector is atached to pipette with a clip.
Push the ejection button to disengage
the ejector from the clip.

2. Push laterally the tip ejector.

3. Slide and remove the tip ejector.

To r efit:

1. Push the ejection button.

2. Slide the tip ejector along the tip holder.

3. Push laterally to clip the tip ejector on
the ejector clip of the pipette.

Single channel models Fixed volume models

Changing the Tip Holder
(No Tools Required)

1. Remove the tip ejector (refer to

Changing the Tip Ejector).

2. Unscrew the connecting nut by turning it
clockwise.

3. Carefully separate the lower and upper parts.

4. Remove the piston assembly and the seals.

5. Clean, autoclave, or replace the tip holder.

6. If necessary, lightly lubricate the piston and its seals.

7. Reassemble the pipette (refer to Figure 18 on page 15).

8. Tighten the connecting nut (turn counterclockwise).

9. Refit the tip ejector (see above).

Figure 17

Removing the tip ejector

1

2

1

2

14

Removing the tip holder could impact pipette per formance. Performance should be checked upon
refitting the new tip holder. Extra care should be taken with the piston for the P2/P 10 volumes,
improper installation can cause critical damage to the piston. Users should also keep track of
seals/O-rings and make sure they are reinstalled properly. Failure to reinstall properly can result in a
leaking pipette.

MAINTENANCE | PIPETMAN® L

Variable volume, single channel models

Color-coded
push button

Thumbwheel

Fixed volume models

Color-coded
push button

Graduated
cover

MAINTENANCE

P10L, P20L

P100L, P200L

Tip holder

P1000L

Connecting

nut

Piston

assembly

Plastic tip

ejector

P5000L
P10mLL

Stainless

steel tip

ejector

Plastic tip

ejector

P5000L
P10mLL

F10L, F20L

F100L, F200L

Tip holder

Connecting

F5000L

nut

F1000L

Piston

assembly

Tip ejector

Figure 18

Piston assembly (disassembled)

Service the Piston Assembly

You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the
pipette must be adjusted and calibrated by trained technician in a Gilson-authorized service center. As
the models P2L, P10L, F1L, F2L, F5L, and F10L contain very small parts, it is best not to disassemble these
pipettes yourself.

1. Remove the tip ejector (refer to Changing the Tip Ejector on page 14).

2. Unscrew the connecting nut by turning it clockwise.

3. Carefully separate the lower and upper parts.

4. Remove the piston assembly, O-ring, and seal.

5. Leave exposed the piston, clean it with isopropanol or ethanol and lubricate
lightly (lubricant P/N: 5440011070).

For P20L, P100L , P200L, F20L , F25L, F50L, F100L, F200L , F250L: lubricate only the
functional part of the piston (25mm ±/-5 mm length) and the O-ring.

For P1000L, F300L, F400L, F500L, and F1000L: lubricate the piston.
For P5000L, P10mLL, and F5000L: disassemble the seals, lubricate the seals internal

part, and lubricate the piston. Do not lubricate the O-ring.

6. Reassemble the pipette (refer to Figure 18).

Figure 19

Hold the piston
assembly

7. Tighten the connecting nut (turn counter-clockwise).

8. Refit the tip ejector (refer to Changing the Tip Ejector on page 14).

PIPETMAN® L | USER’S GUIDE

15

Replacing the Seal and O-ring

NOTE

NOTE

The O-ring and seal are on the piston; if worn or damaged in any way (chemical or mechanical), they must
be replaced. As the models P2L and P10L, F1L, F2L, F5L and F10L contain very small parts, it is best not to
disassemble these pipettes yourself. Please contact your local Gilson-authorized service center.

Gilson recommends replacement annually, but this can vary depending on use or if the seal/O-ring
is contaminated or damaged.

The dimensions of the O-ring vary depending on the model of pipette.

1. Remove the tip ejector (refer to Changing the Tip Ejector on page 14).

2. Unscrew the connecting nut by turning it clockwise.

3. Carefully separate the lower and upper parts.

4. Remove the piston assembly, O-ring, and seal.

5. If necessary, clean the piston and replace the seal and O-ring; lubricate them lightly.
Please place them in the correct order.

6. Reassemble the pipette (refer to the Figure 18 on page 15).

MAINTENANCE

7. Tighten the connecting nut (turn counter-clockwise).

8. Refit the tip ejector (refer to Changing the Tip Ejector on page 14).

Maintenance For Multichannel Models

Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of
performance.

Maintenance is limited to:

● Cleaning or decontamination (refer to CLEANING AND DECONTAMINATION on page 17)

● Replacing spare parts

● Greasing the piston assembly

Changing the Tip Ejector for Multichannel Models

1. To remove the tip ejector, keep both ejector locks depressed. Pull the tip ejector down.

2. To refit the tip ejector, gently re-insert the tip ejector vertically into the rails of the ejector support.
Pull lightly on the tip ejector to check the position.

Tip ejector for all models Tip ejector for P8x1200L

and P12x1200L

Figure 20

PIPETMAN® L multichannel tip ejector and ejector spacers

Changing the V-ring for VR Multichannel Models

P8x200LVR, P12x200LVR, P8x300LVR and P12x300LVR, are equipped with V-rings at the bottom of the
tip holders. V-rings could be in direct contact with the liquids and must be replaced if worn or damaged in
any way. Defective V-rings would result in the incorrect positioning of the tips and in dispensing errors.

Each box of VR multichannel pipette contains one V-ring removal tool and four extra V-rings.

V-rings are proposed for a quick repair but they must be changed regularly during maintenance
and service procedures. V-rings and removal tools are also available as spare parts. Refer to

Variable Volume Multichannel Pipettes Spare Parts, on page 20.

REMOVING THE V-RING FROM THE TIP HOLDER

1. Remove the plastic tip ejector from the pipette.

2. Insert the V-ring removal tool (included in the pipette
box) at the bottom end of the tip holder A.

3. Push the tool firmly against the tip holder to release the
V-ring.

4. With your thumb, press the V-ring against the tool B
and push it back down C.

A

B

C

INSERTING THE V-RING ON THE TIP HOLDER

1. Hold the V-ring on both sides with your fingers and slide it on the
tip holder, side B of the V-ring facing the tip holder A.

2. Turn the V-ring using your finger or a tip to turn and place the
V-ring B.

3. Check that the pipette tips are correctly positioned.

4. Ensure that the tips are tightly sealed and properly aligned.

MAINTENANCE | PIPETMAN® L

16

A B

Side B

Side A

Correct

Placement

Incorrect

Placement

Chapter 8

NOTICE

NOTICE

CLEANING AND DECONTAMINATION

PIPETMAN L is designed so that the parts normally in contact with liquid contaminants can easily be
cleaned and decontaminated, and the variable volume models are autoclavable without disassembly.
However, because the P2L, P10L, F1L, F2L, F5L, and F10L contain very small parts, it is best not to
disassemble these pipettes yourself. Please contact your local Gilson-authorized Service Center.

You may refer to the decontamination procedure available on the Gilson website (click here to fi nd

Gilson resource center).

Liquid must never enter the upper part (handle) of any pipette.

Cleaning for Single Channel and Fixed Volume Models

The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is
recommended for cleaning PIPETMAN L.

External

1. Remove the tip ejector (refer to changing the tip ejector for single channel and fixed volume models).

2. Wipe the entire pipette and the tip ejector with a soft-cloth or lint-free tissue soaked with soap solution to
remove all dirty spots or marks. If the pipette is very dirty, a brush with sof t, plastic bristles may be used.

3. Wipe the entire pipette and the tip ejector with a soft cloth or lint-free tissue soaked with distilled water.

4. Refit the tip ejector and allow the pipette to dry.

Internal

Only the following components can be immersed in a cleaning solution: connecting nut, tip ejector, tip
holder, piston assembly, seal, and O-ring.

1. Disassemble the pipette (refer to Maintenance for Single Channel, Variable, and Fixed Volume Models
on page 14).

2. Set aside the upper part in a clean, dry place.

3. Clean the individual components of the lower part of the pipette using an ultrasonic bath (20 minutes
at 50°C) or with a soft-cloth and brushes. Note that the piston assembly and seals must be degreased
with isopropanol or ethanol before being immersed in another ultra sonic bath. Small round brushes
with soft plastic bristles may be used to clean the interior of the tip holder.

4. Rinse the individual components with distilled water.

5. Leave the parts to dry by evaporation or wipe them with a clean, soft cloth or lint-free tissue.

6. Reassemble the pipette (refer to Maintenance for Single and Fixed Volume Models on page 14).

CLEANING AND DECONTAMINATION

Cleaning for Multichannel Models

Only the following components can be immersed in a cleaning solution: tip ejector, ejector locks, and

ejector spacer.

1. Remove the tip ejector and the ejector spacer.

2. Immerse the tip ejector, ejector locks and ejector spacer in the cleaning solution or wipe them with a
soft cloth or lint-free tissue soaked with the cleaning solution.

3. Rinse the components with distilled water.

4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the cleaning solution.

5. Wipe it with distilled water.

6. Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue.

7. Refit the tip ejector (refer to Changing the Tip Ejector on page 14).

Ejector locks

Tip ejector

Ejector locks

Tip ejector

Ejector spacer

Ejector spacer

Ejector locks

Tip ejector

Ejector spacer

PIPETMAN® L | USER’S GUIDE

17

CLEANING AND DECONTAMINATION

NOTE

Autoclaving

For Variable Volume (Single and Multichannel Models)

PIPETMAN L variable volume models with serial number starting from NK or newer versions featuring
steam–sterilization logo are fully autoclavable without disassembly for maximum convenience as well as

protection from contamination.

1. Before autoclaving

It is possible to clean PIPETMAN L pipettes and grease the piston prior to autoclaving; however, if you
remove existing grease, lightly lubricate the piston seal using only the grease specified in this User’s Guide.
Set the pipette volume to the nominal volume before placing it in the autoclave.

2. Procedure

PIPETMAN L variable volume models with a serial number that begins with NK or newer versions featuring
steam–sterilization logo can be sterilized by steam autoclaving at 121°C (252°F), 1 bar relative pressure, for
20 minutes without disassembly.

When autoclaving, the pipette will dry better and faster without using a bag.

3. After autoclaving

Following the autoclaving cycle, leave the pipette to cool down overnight to room temperature and dry
completely before use.

Checking should be carried out according to your standard operating procedure. Leak test may also
be performed to ensure that the pipette does not leak, as described in TROUBLESHOOTING, part Leak
Test on page 13.

For Fixed Volume Models

The upper part (body) and the piston assembly of the pipette are not autoclavable. Only the following
parts may be autoclaved: tip ejector, tip holder, and connecting nut.

Chemical Decontamination

You may choose to decontaminate your pipette chemically, in accordance with your own procedures.
Whatever decontaminant you use, check with the supplier of the decontaminant that it is compatible
with stainless steel and the plastics used in the construction of the pipette: PA (Polyamide), PBT
(Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephtalate),
POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), PP (Polypropylene), PPA (Polyphthalamide), or
PPS (Polyphenylene Sulfide).

Chemical Decontamination for Variable Volume Single and Fixed Models

Upper Part (Handle)

1. Wipe the upper part (handle) of the pipette with a soft cloth or lint-free tissue soaked with the chosen
decontaminant.

2. Wipe the upper part of the pipette with a soft cloth or lint-free tissue soaked with distilled water or
sterile water.

Lower Part (Volumetric module)

Only the following components can be immersed in a decontaminant solution: connecting nut, tip ejector,
and tip holder.

Piston assembly and seals must be degreased with isopropanol or ethanol in a separate vessel
before being immersed in decontamination solution.

1. Disassemble the pipette (refer to MAINTENANCE on page 14).

2. Immerse tip ejector, tip holder and connecting nut in the cleaning solution.

3. Degrease the piston assembly and the seals and then immerse them in another vessel.

4. Rinse each component with distilled water.

5. Leave the parts to dry by evaporation (or wipe with a soft cloth the tip ejector, the tip holder, and
connecting nut).

6. Lubricate the piston assembly and the seals.

7. Reassemble the piston assembly, the tip holder, and the tip ejector.

For Multichannel Models

Only the following components can be immersed in a decontamination solution: tip ejector, ejector locks,
and ejector spacer.

1. Remove the tip ejector and the ejector spacer.

2. Immerse the tip ejector, ejector locks, and ejector spacer in the decontamination solution or wipe
them with a soft cloth or lint-free tissue soaked with the decontamination solution.

3. Rinse the components with distilled water.

4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the decontamination solution.

5. Wipe it with distilled water.

6. Leave the parts to dry by evaporation or wipe them with a clean, soft cloth or lint-free tissue.

7. Refit the tip ejector (refer to Changing the Tip Ejector for Multichannel on page 16).

CLEANING AND DECONTAMINATION | PIPETMAN® L

18

Chapter 9

NOTE

NOTE

SPARE PARTS

Variable Volume, Single Channel Models

► Service Kit 1st level includes:

○ Three piston seals or seal guides*
○ Three O-rings*
○ One tip holder

D

E

► Service Kit 2nd level includes:

○ One push button
○ One connecting nut
○ One tip ejector

A

B

F1 F2

► Or only for P2L and P10L

○ One adapter for stainless steel tip ejector
○ One extension for plastic tip ejector

P2L (FA10001P OR M) and
P10L(FA10002P OR M)

Label Description P2L P10L

C+D+E Service Kit 1stlevel F144501 FA07001

A+B+F1
to F4

C+D

F3 Tip ejector adapter F144879 F144879

F4

Service Kit 2ndlevel FA07301 FA07302

Seal + O-Ring
(5sets)

Tip ejector
extension

F144861 FA07012

F2070903 F2070903

C

F3

F4

SPARE PARTS

A

B

C

D

P20L (FA10003P OR M) and
P100L(FA10004P OR M)

Label Description P20L P100L

C+D+E Service Kit 1st level FA07002 FA07003

A+B+
F1+F2

C+D

Service Kit 2ndlevel FA07303 FA07304

Seal guide + O-ring
(5sets)

FA07013 FA07014

P200L (FA10005P OR M) and
P1000L(FA10006P OR M)

Label Description P200L P1000L

C+D+E Service Kit 1st level FA07004 FA07005

A+B+
F1+F2

C+D

Service Kit 2ndlevel FA07305 FA07306

Seal guide + O-ring
(5sets)

FA07015 FA07016

P5000L (FA10007) and P10MLL (FA10008)

Label Description P5000L P10MLL

C+D+E Service Kit 1st level FA07311 FA07312

A Push button F107009 F107010

F5 Tip ejector FA07377 FA07378

C+D

Seal guide + O-ring
(5sets)

FA07307 FA07308

All Models

Description Part Number

Lubricant 5440011070

F1

F4

*For P5000L, two piston seals and O-rings are provided on
the service Kit 1st level.

F2

F3

F5

Each single channel pipette model
(except P5000L and P 10m LL) has
two dierent ordering references
to identif y the kind of tip ejector
required. For a pipette with a plastic
tip ejector, the ordering reference
ends with the letter P, for a pipette
with a stainless steel tip ejector, the
ordering reference ends with the
letter M .

Ex: For a P 10L with the plastic tip
ejector the ordering reference is
FA10002P. For the same pipette with a
stainless steel tip ejector, the ordering
reference is FA10002M.

P5000L and P10mLL are equipped
with a plastic tip ejector.

F1

E

F2

PIPETMAN® L | USER’S GUIDE

19

SPARE PARTS

Variable Volume, Multichannel Models

F3

Tip ejector (8 channels)

H3

Plastic ejector spacer D10 (8 channels)

P8X10L (FA10013) AND P12X10L(FA10014)

Label Description 8X10 12X10

F3 - F4 Tip ejector FA0 73 75 FA07376

H3 - H4

Plastic ejector spacer
D10

FA07360 FA07361

P8X20L (FA10009) AND P12X20L(FA10010)

Label Description 8X20 12X20

F3 - F4 Tip ejector FA0 73 75 FA07376

H4

Plastic ejector spacer D10 (12 channels)

F4

Tip ejector (12 channels)

V-ring for VR
multichannel
models only

F6

V-ring removal

F5

tool

P8X200L (FA10011), P8X200LVR (FA10035),
AND P12X200L (FA10012), P12X200LVR (FA10036)

Label D escription 8X200 12X200

F3 - F4 Tip ejector FA0 73 75 FA07376

F5 V-ring (24 units) FA07381 FA07381

F6 V-ring removal tool FA07382 FA07382

F5+F6

V-ring (24 units) +
V-ring removal tool

FA07384 FA07384

P8X300L (FA10015), P8X300LVR (FA10037),
AND P12X300L (FA10016), P12X300LVR (FA10038)

Label Description 8X300 12X300

F3 - F4 Tip ejector FA07375 FA07376

F5 V-ring (24 units) FA07381 FA07381

F6 V-ring removal tool FA07382 FA07382

F5+F6

V-ring (24 units) +
V-ring removal tool

FA07384 FA07384

P8X1200L (FA10039) AND P12X1200L (FA10040)

Label Description 8X1200 12X31200

G Ejector lock F507008 F507008

H1-H2 Ejector spacer F807139 F807140

F7-F8 Tip ejector FA07099 FA07100

H1

Ejector spacer
(8 channels)

G

F7

Ejector lock

Tip ejector
(8 channels)

Tip ejector

F8

(12 channels)

Ejector lock

G

SPARE PARTS | PIPETMAN® L

20

H2

Ejector spacer
(12 channels)

Fixed Volume, Single Channel Models

► Service Kit 1st level includes:

○ Three piston seals or seal guides*
○ Three O-rings*
○ One tip holder

D

E

► Service Kit 2nd level includes:

○ One push button
○ One connecting nut
○ One tip ejector

A

B

F1

► Or only for the F1L, F2L, F5L, and F10L

○ One extension for plastic tip ejector

F1L (FA10017), F2L (FA10018), F5L (FA10019),
AND F10L (FA10020)

Label Description F1L/F2L F5L/F10L

C+D+E Service Kit 1

A+B+
F1+F4

C+D

F4

Service Kit 2nd level FA07067 FA07068

Seal Guide + O-ring
(5 sets)

Tip Ejector
Extension

st

level F144501 FA07001

F144861 FA07012

F2070903 F2070903

C

F4

A

SPARE PARTS

B

C

D

F1

F20L (FA10021), F25L (FA10022),
F50L (FA10023), AND F100L (FA10024)

Label Description F20L/F25L

C+D+E Service Kit 1st level FA07002 FA07003

A+B+F1 Service Kit 2nd level FA07069 FA07070

C+D

Piston Seal+ O-ring
(5 sets)

FA07013 FA07014

F50L/
F100L

F4

*For F5000L, two piston seals and O-rings
are provided on the Service Kit 1

F200L (FA10025), F250L (FA10026)

Label Description F200L F250L

C+D+E Service Kit 1st level FA07004 FA07004

A+B+F1 Service Kit 2nd level FA07071 FA07073

C+D

Piston Seal + O-ring
(5 sets)

FA07015 FA07015

F300L (FA10027), F400L (FA10028), F500L (FA10029),
F1000L (FA10030), AND F5000L (FA10031)

F300L

Label Description

C+D+E Service Kit 1st level FA07005 FA07021

F400L
F500L
F1000L

F5000L

F1

E

st

level

A+B+F1 Service Kit 2nd level FA07072 NA

C+D

Piston Seal + O-ring
(5 sets)

FA07016 FA07017

All Models

Description Part Number

Lubricant 5440011070

PIPETMAN® L | USER’S GUIDE

21

NOTICE

WARRANTY

Chapter 10

WARRANTY

Gilson warrants this pipette against defects in material under normal use and service for a period of

three years from the date of purchase.

This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or
inadequate maintenance (contrary to the recommendations given in the user’s guide), including, but
not limited to pipettes which have been subjected to physical damage, improper handling, or spillage
or exposure to any corrosive environment. This warranty shall also be void in the event pipettes are
altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this
warranty shall be limited to, at Gilson’s sole option , repair or replacement of any defective components
of pipettes or refund of the purchase price paid for such pipettes. Routine cleaning, control, and

recalibration are not covered under the warranty. The replacement of wearing parts such as seals,
O-rings, broken pistons assembly, and broken tip holders are not covered under the warranty.

Yearly routine maintenance is highly recommended to keep your pipette in good condition, ensuring
a continued high level of performance.

THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER
WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF
MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE,
UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE,
INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR
IMPLIED WARRANTY

22

WARRANTY | PIPETMAN® L

NOTES

NOTES

PIPETMAN® L | USER’S GUIDE

23

Specifications subject to change without notification —
errors excepted.

gilson.com/contactus

LT8 0 157 5/G | ©2022 Gilson, Inc. All rights reserved.